Publications by authors named "Yawen Wang"

212 Publications

miR-198 inhibits the progression of renal cell carcinoma by targeting BIRC5.

Cancer Cell Int 2021 Jul 21;21(1):390. Epub 2021 Jul 21.

Hubei Key Laboratory for Kidney Disease Pathogenesis and Intervention, Hubei Polytechnic University School of Medicine, Xialu District guilin north, road no. 16, Huangshi, 435003, Hubei, China.

Background: miR-198 is involved in the formation, migration, invasion, and metastasis of various malignant cancers. However, the function and mechanism of action of miR-198 in the tumorigenesis of renal cell carcinoma (RCC) remain elusive. Here, we aimed to explore the role of miR198 in RCC.

Methods: Immunohistochemistry was performed to estimate the level of survivin in RCC sections. Quantitative real-time polymerase chain reaction was performed to determine the expression level of miR-198 in fresh RCC tissues. Furthermore, the target relationship between miR-198 and BIRC5 was predicted using the TargetScanHuman 7.2 database and verified via dual-luciferase reporter assay and western blotting. The effects of miR-198 on the viability, apoptosis, invasion, and migration of A498 and ACHN cells were studied using Cell Counting Kit-8, flow cytometry, transwell migration assay, and wound healing assay, respectively. Additionally, a xenograft nude mouse model was established to evaluate the effect of miR-198 on RCC tumorigenesis.

Results: The expression levels of BIRC5 and miR-198 were respectively higher and lower in RCC tissues than those in normal adjacent tissues. Furthermore, miR-198 could inhibit luciferase activity and reduce the protein level of survivin without affecting the BIRC5 mRNA levels. miR-198 inhibited cell viability, migration, and invasion and promoted cell apoptosis; co-transfection with BIRC5 could rescue these effects. Moreover, miR-198 could repress tumor growth in the xenograft nude mouse model of RCC.

Conclusions: Our study demonstrates that miR-198 suppresses RCC progression by targeting BIRC5.
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http://dx.doi.org/10.1186/s12935-021-02092-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296723PMC
July 2021

Handgrip strength as a predictor of incident hypertension in the middle-aged and older population: The TCLSIH cohort study.

Maturitas 2021 Aug 11;150:7-13. Epub 2021 Jun 11.

Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China; Nutritional Epidemiology Institute and School of Public Health, Tianjin Medical University, Tianjin, China; Health Management Centre, Tianjin Medical University General Hospital, Tianjin, China; Tianjin Key Laboratory of Environment, Nutrition and Public Health, Tianjin, China; Center for International Collaborative Research on Environment, Nutrition and Public Health, Tianjin, China. Electronic address:

Objectives: The independent role of muscular strength in the prevention of chronic disease is increasingly being recognized. However, no cohort study has assessed the relationship between handgrip strength and the incidence of hypertension among the middle-aged and older population. The aim of this prospective cohort study was to investigate whether handgrip strength is related to incident hypertension among people aged 40 years and over.

Study Design: This prospective cohort study (n = 8,480) was performed between 2013 and 2019 as part of the Tianjin Chronic Low-grade Systemic Inflammation and Health (TCLSIH) Cohort Study, Tianjin, China.

Main Outcome Measures: Participants without baseline hypertension were followed up for ~6 years (median 4.0 years). Hypertension was defined according to the JNC7 criteria. Handgrip strength was measured using a hydraulic handheld dynamometer. Adjusted Cox proportional hazards regression models were used to assess the relationships between weight-adjusted handgrip strength and the risk of incident hypertension.

Results: The incidence rate of hypertension per 1000 person-years was 70. The fully adjusted hazards ratios (95% confidence interval) of the incidence of hypertension for increasing quartiles of weight-adjusted handgrip strength were: 1.00(reference), 0.84 (0.75-0.95), 0.78 (0.69-0.88), and 0.66 (0.58-0.75) (P for trend<0.0001). Moreover, the adjusted hazards ratio (95% confidence interval) of incident hypertension for per unit increase in weight-adjusted handgrip strength was 0.17 (0.10-0.27) (P<0.0001). Similar results were observed in males and females.

Conclusions: The present cohort study is the first to find that high weight-adjusted handgrip strength, but not absolute handgrip strength, is significantly and independently related to low risk of incident hypertension among the middle-aged and older population.
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http://dx.doi.org/10.1016/j.maturitas.2021.06.002DOI Listing
August 2021

Identification of C-glycosyl flavones and O-glycosyl flavones in five Dendrobium species by HPLC coupled with electrospray ionization multi-stage tandem MS.

Rapid Commun Mass Spectrom 2021 Jul 5:e9158. Epub 2021 Jul 5.

The First College of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China.

Rationale: Flavones are widely used in traditional Chinese medicine (TCM) and are the pharmacologically active ingredients of many medicinal plants, such as Dendrobium. With the increasing demand for medicinal Dendrobium, the identification of characteristic flavones that can serve as chemical markers for quality control is critical for quality assurance and safety in the TCM industry.

Methods: High-performance liquid chromatography coupled with electrospray ionisation multistage tandem mass spectrometry (HPLC-ESI-MS/MS) was used to identify the chemical constituents in five types of Dendrobium: D. crystallinum, D. falconeri, D. strongylanthum, D. moniliforme, and D. gratiosissimum.

Results: A total of seventy-six C-glycosyl flavones and three O-glycosyl flavones were identified, of which fifteen C-glycosyl flavones were found in D. crystallinum, twenty four were found in D. falconeri, thirty were found in D. strongylanthum, seven were found in D. moniliforme (also called "Huangtongpi", from Anhui, China), fifteen were found in D. moniliforme (also called "Zitongpi", from Yunnan, China) and seventeen were found in D. gratiosissimum. Additionally, three flavone O-glycosides were found in D. strongylanthum.

Conclusions: The results of this study may be useful for quality assessment and for the application of D. crystallinum, D. falconeri, D. strongylanthum, D. moniliforme, D. gratiosissimum. This study provides comprehensive information for the identification of flavones from other Chinese herbs.
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http://dx.doi.org/10.1002/rcm.9158DOI Listing
July 2021

Association of Appendicular Skeletal Muscle to Trunk Fat Ratio with Type 2 Diabetes Mellitus in Older Adults.

Gerontology 2021 Jun 30:1-8. Epub 2021 Jun 30.

Nutritional Epidemiology Institute and School of Public Health, Tianjin Medical University, Tianjin, China.

Introduction: Mounting evidence has demonstrated that skeletal muscle and visceral adiposity play crucial roles in glucose metabolism. The purpose of this study was to investigate whether the appendicular skeletal muscle mass index (ASMI) to trunk fat mass (TFM) ratio (ASMI/TFM) is a more specific and identifiable factor for type 2 diabetes mellitus (T2DM) in older adults than conventional anthropometric measures.

Methods: This cross-sectional study included 1,370 older adults from the Tianjin Chronic Low-Grade Systemic Inflammation and Health (TCLSIH) cohort. ASMI and TFM were measured by using a bioelectrical impedance analyzer, and T2DM was defined with the criteria of the American Diabetes Association. Odds ratios (ORs) were evaluated using multivariable logistic analysis.

Results: The prevalence of T2DM is 20.0% in this study. The multivariable-adjusted ORs (95% confidence interval) of T2DM for increasing categories of ASMI/TFM, BMI, and waist circumference (WC) were 1.00 (reference), 0.70 (0.49, 1.02), 0.61 (0.42, 0.89), and 0.45 (0.30, 0.67; p for trend <0.0001); 1.00 (reference), 1.15 (0.83, 1.60), and 1.37 (0.94, 2.01; p for trend = 0.10); and 1.00 (reference) and 1.78 (1.19, 2.74; p < 0.01), respectively.

Conclusions: Higher ASMI/TFM was associated with a lower prevalence of T2DM in this study of older adults. The T2DM predictive value of ASMI/TFM may be stronger than BMI and WC in this population.
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http://dx.doi.org/10.1159/000516076DOI Listing
June 2021

Atomically dispersed Palladium-Ethylene Glycol- Bismuth oxybromide for photocatalytic nitrogen fixation: Insight of molecular bridge mechanism.

J Colloid Interface Sci 2021 Jun 22;603:17-24. Epub 2021 Jun 22.

Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences 030032 PR China.

Performance of single-atom catalysis largely depends on the interaction between the metal and the supporter. Herein, ethylene glycol (EG) was used as a molecular bridge connecting Palladium (Pd) and bismuth oxybromide (BiOBr) to form atomically dispersed Pd catalyst (Pd-EG-BiOBr) for photocatalytic nitrogen fixation under ambient conditions. Compared with 0.20 wt% Pd-BiOBr, 0.20 wt% Pd-EG-BiOBr greatly promoted the photocatalytic nitrogen fixation activity, affording an ammonia formation rate of 124.63 μmol·h. The molecular bridge mechanism during catalyst formation and photocatalysis is speculated based on Transmission electron microscopy, In-situ Fourier transform infrared spectra, Electron spin resonance spectra, UV-vis diffuse reflectance spectra, Photoluminescence spectra and Density Functional Theory calculations. The results show that EG not only induces the formation of atomically dispersed Pd, but also enhances the electron density of Pd and activation capacity of nitrogen molecules. This work opens a new door to applications of atomically dispersed Pd supported catalysts for high efficiency photocatalytic nitrogen fixation.
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http://dx.doi.org/10.1016/j.jcis.2021.06.108DOI Listing
June 2021

Efficient removal of emerging contaminant sulfamethoxazole in water by ozone coupled with calcium peroxide: Mechanism and toxicity assessment.

Chemosphere 2021 Jun 11;283:131156. Epub 2021 Jun 11.

College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.

Sulfamethoxazole (SMX) is a widely distributed emerging contaminant, which will bring serious harm to ecology and human health. Herein, evaluation of ozone (O) coupled with calcium peroxide (CaO) for SMX elimination was carried out. The results showed that CaO could promote SMX elimination in O system. The removal efficiency was improved from 65.6% to 73.9% when the CaO dosage was 0.06 g L. O dosage of 0.55 g h was beneficial to SMX degradation. With decrease of initial SMX concentration, the removal of SMX firstly enhanced and then declined. Compared with alkaline, acidic and neutral conditions were favorable for SMX degradation. ROS including ·OH, ·O and O play critical role for SMX degradation. Synergetic effect could be established between O and CaO, which encouraged formation of ·OH and accelerated SXM decomposition. The total organic carbon (TOC) and chemical oxygen demand (COD) were all declined after O/CaO treatment. According to results of liquid chromatography-mass spectrometry (LC-MS) and references, four major pathways were proposed. The O/CaO technology was also suitable for practical wastewater treatment. QSAR calculation and seed germination experiment showed that toxicity of the treatment solution was alleviated after O/CaO treatment.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131156DOI Listing
June 2021

A Bacteriophage DNA Mimic Protein Employs a Non-specific Strategy to Inhibit the Bacterial RNA Polymerase.

Front Microbiol 2021 2;12:692512. Epub 2021 Jun 2.

BioBank, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

DNA mimicry by proteins is a strategy that employed by some proteins to occupy the binding sites of the DNA-binding proteins and deny further access to these sites by DNA. Such proteins have been found in bacteriophage, eukaryotic virus, prokaryotic, and eukaryotic cells to imitate non-coding functions of DNA. Here, we report another phage protein Gp44 from bacteriophage SPO1 of , employing mimicry as part of unusual strategy to inhibit host RNA polymerase. Consisting of three simple domains, Gp44 contains a DNA binding motif, a flexible DNA mimic domain and a random-coiled domain. Gp44 is able to anchor to host genome and interact bacterial RNA polymerase the β and β' subunit, resulting in bacterial growth inhibition. Our findings represent a non-specific strategy that SPO1 phage uses to target different bacterial transcription machinery regardless of the structural variations of RNA polymerases. This feature may have potential applications like generation of genetic engineered phages with Gp44 gene incorporated used in phage therapy to target a range of bacterial hosts.
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http://dx.doi.org/10.3389/fmicb.2021.692512DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208478PMC
June 2021

Transumbilical laparoendoscopic single-site surgery (TU-LESS) extraperitoneal approach for lymphadenectomy: an innovative method.

J Gynecol Oncol 2021 May 11. Epub 2021 May 11.

Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, China.

Objective: In gynecological oncology surgery, pelvic lymphadenectomy and para-aortic lymphadenectomy is a critical surgical staging procedure. Multiple methods have been used to perform lymphadenectomy. Compared with multiport laparoscopy, the central incision of transumbilical laparoendoscopic single-site surgery (TU-LESS) provides equal access to the para-aortic and bilateral obturator region [1]. And it is accepted that extraperitoneal approach is ideal for lymphadenectomy, which avoids intestinal disturbance and reduce intra-abdominal adhesion [2]. Therefore, we developed TU-LESS extraperitoneal approach for lymphadenectomy, which combined the benefits of these aforementioned methods.

Methods: This study was approved by the Ethics Committee of West China Second University Hospital of Sichuan University (No. 150). A video is applied to demonstrate each steps for this specific technique. Step 1: Create TU-LESS intraperitoneal approach. Step 2: Pinpoint the peritoneal position. Step 3: Insert the port and create retroperitoneal pneumonia. Step 4: Remove lymph nodes through TU-LESS extraperitoneal approach. Step 5: Operate intraperitoneal surgery.

Results: This micro-invasive approach reduces the risk of direct bowel injury, adhesion formation, wound complications. For gynecologic cancer patients, especially for the advanced cervical cancer, this micro-invasive approach not only provide accurate staging but also achieve enhanced recovery following surgery, thus patients could receive subsequent adjuvant radio/chemotherapy on time.

Conclusion: TU-LESS extraperitoneal approach is an innovative method for lymphadenectomy.
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http://dx.doi.org/10.3802/jgo.2021.32.e69DOI Listing
May 2021

Association between consumption of ultra-processed foods and hyperuricemia: TCLSIH prospective cohort study.

Nutr Metab Cardiovasc Dis 2021 Jun 7;31(7):1993-2003. Epub 2021 May 7.

Nutritional Epidemiology Institute and School of Public Health, Tianjin Medical University, Tianjin, China; Health Management Centre, Tianjin Medical University General Hospital, Tianjin, China; Tianjin Key Laboratory of Environment, Nutrition and Public Health, Tianjin, China; Center for International Collaborative Research on Environment, Nutrition and Public Health, Tianjin, China. Electronic address:

Background And Aims: Emerging evidence suggests that consumption of ultra-processed foods (UPF) plays a role in the development of chronic diseases, but evidence of their influence on hyperuricemia is limited. We therefore designed a cohort study to examine whether UPF consumption increase the risk of hyperuricemia in adults.

Methods And Results: This was a prospective study (n = 18,444) performed in Tianjin, China from 2013 to 2019. Participants that were aged 18 years and over and with no history of hyperuricemia, were followed up for 1-6 years (median follow-up duration = 4.2 years). UPF consumption was assessed by a validated semi-quantitative food frequency questionnaire. Hyperuricemia was defined as serum uric acid levels ≥7.0 mg/dL in males and ≥ 6 mg/dL in females. Multivariable Cox proportional hazards regression models were used to assess the association between UPF consumption and the risk of hyperuricemia. Restricted cubic spline regression was used to estimate the dose-response association between UPF consumption and risk of hyperuricemia. During follow-up period, the incidence of hyperuricemia was 20.3% in general population (27.7% in males and 13.2% in females). In the final multivariate models, the hazard ratios (95% confidence interval) for hyperuricemia across energy adjusted UPF consumption quartiles were 1.00 (reference), 1.04 (0.94, 1.14), 1.11 (1.01, 1.23), 1.16 (1.05, 1.28) (p for trend = 0.02) in general population.

Conclusions: This population-based prospective cohort study suggests that increased consumption of UPF is independently associated the risk of hyperuricemia.
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http://dx.doi.org/10.1016/j.numecd.2021.04.001DOI Listing
June 2021

3D Anisotropic [email protected] Hemispherical Nanostructures as Efficient Electrocatalysts for Methanol, Ethanol, and Formic Acid Oxidation Reaction.

Adv Mater 2021 Jul 10;33(30):e2100713. Epub 2021 Jun 10.

Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu, 215123, P. R. China.

Anisotropic 3D nanostructures exhibit excellent electrocatalytic activity and stability due to their heterogeneous elemental distribution and unsymmetrical configuration. However, it is still a huge challenge to combine anisotropically distributed elements and anisotropic morphologies within one 3D nanostructure. Herein, 3D [email protected] hemispherical nanostructures ([email protected] H-Ss) are fabricated as highly efficient electrocatalysts for oxidation reaction, which present heterogenous element distribution and anisotropic morphology. It is demonstrated that the non-uniform adsorption of BO on Au-CTA surface, as well as the simulated lower formation energy of Pt-Pd atoms for Au-CTA -BO , basically contribute to the eventual formation of [email protected] H-Ss. Impressively, the unique anisotropic [email protected] H-Ss exhibit superior electrocatalytic activity and durability for methanol, ethanol, and formic acid oxidation reaction compared with commercial Pt/C and previously reported noble-metal based electrocatalysts. Especially, the mass activity of [email protected] H-Ss for MOR is 4.38 A mg , which is about 2.0 and 4.7 times that of [email protected] spherical nanostructures ([email protected] Ss) and commercial Pt/C catalyst, respectively. This work provides an important reference for the design and preparation of 3D anisotropic and high-efficiency electrocatalysts.
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http://dx.doi.org/10.1002/adma.202100713DOI Listing
July 2021

TLR7 Signaling Drives the Development of Sjögren's Syndrome.

Front Immunol 2021 24;12:676010. Epub 2021 May 24.

Aix Marseille Univ, CNRS, INSERM, CIML, Marseille, France.

Sjögren's syndrome (SS) is a chronic systemic autoimmune disease that affects predominately salivary and lacrimal glands. SS can occur alone or in combination with another autoimmune disease like systemic lupus erythematosus (SLE). Here we report that TLR7 signaling drives the development of SS since TLR8-deficient (TLR8ko) mice that develop lupus due to increased TLR7 signaling by dendritic cells, also develop an age-dependent secondary pathology similar to associated SS. The SS phenotype in TLR8ko mice is manifested by sialadenitis, increased anti-SSA and anti-SSB autoantibody production, immune complex deposition and increased cytokine production in salivary glands, as well as lung inflammation. Moreover, ectopic lymphoid structures characterized by B/T aggregates, formation of high endothelial venules and the presence of dendritic cells are formed in the salivary glands of TLR8ko mice. Interestingly, all these phenotypes are abrogated in double TLR7/8-deficient mice, suggesting that the SS phenotype in TLR8-deficient mice is TLR7-dependent. In addition, evaluation of TLR7 and inflammatory markers in the salivary glands of primary SS patients revealed significantly increased expression levels compared to healthy individuals, that were positively correlated to , , and expression. These findings establish an important role of TLR7 signaling for local and systemic SS disease manifestations, and inhibition of such will likely have therapeutic value.
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http://dx.doi.org/10.3389/fimmu.2021.676010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183380PMC
May 2021

Saltwater fish but not freshwater fish consumption is positively related to handgrip strength: The TCLSIH Cohort Study.

Nutr Res 2021 06 25;90:46-54. Epub 2021 Apr 25.

Nutritional Epidemiology Institute and School of Public Health, Tianjin Medical University, Tianjin, China; Tianjin Key Laboratory of Environment, Nutrition and Public Health, Tianjin, China; Center for International Collaborative Research on Environment, Nutrition and Public Health, Tianjin, China; Health Management Centre, Tianjin Medical University General Hospital, Tianjin, China. Electronic address:

Fish contain many important nutrients and are primarily known for high n-3 polyunsaturated fatty acids (n-3 PUFA) content. Studies have shown that supplementation of fish oil-derived n-3 PUFA improves muscle mass and strength. Here, we hypothesized that fish consumption might improve muscle strength. To test this hypothesis, we performed this cross-sectional study (n = 29,084) in Tianjin, China. The frequency of fish consumption was assessed using a valid self-administered food frequency questionnaire. Handgrip strength (HGS) was used as the indicator of muscle strength, and was measured using a handheld digital dynamometer. Analysis of covariance was used to examine the relationship between fish consumption and HGS. In men, after adjusted potential confounding factors, the least square means (95% confidence intervals) of HGS across saltwater fish consumption categories were 41.5 (41.1, 43.7) kg for <1 time/week, 44.6 (43.2, 45.8) kg for 1 time/week, and 44.7 (43.3, 46.1) kg for ≥2 to 3 times/week (P for trend <0.001). In men, the least square means (95% confidence intervals) of HGS across the ascending quartiles of dietary n-3 PUFA intake were 43.6 (43.2, 44.4) kg, 43.7 (43.2, 44.6) kg, 44.4 (43.0, 45.8) kg, and 44.6 (43.1, 46.0) kg (P for trend <0.01). The results showed that saltwater fish consumption was positively related to HGS in men, but not in women, suggesting that saltwater fish contain nutrients that may be used to improve HGS.
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http://dx.doi.org/10.1016/j.nutres.2021.04.002DOI Listing
June 2021

Printed Honeycomb-Structured Reduced Graphene Oxide Film for Efficient and Continuous Evaporation-Driven Electricity Generation from Salt Solution.

ACS Appl Mater Interfaces 2021 Jun 4;13(23):26989-26997. Epub 2021 Jun 4.

Institute of Functional Nano and Soft Materials (FUNSOM) and Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou, Jiangsu 215123, China.

Water-evaporation-induced electricity generation provides an ideal strategy to solve growing energy demand and supply power for self-powered systems because of its advantages of a highly spontaneous process, continuous power generation, and low cost. However, the reported evaporation-induced generators are limited to working only in deionized (DI) water, leading to a low output power. Herein, we utilize a modified multiple ion mode to demonstrate that the streaming potential can be optimized in microchannels filled with salt solution and achieve an enhanced evaporation-induced output power in salt solution by a generator based on honeycomb-structured reduced graphene oxide (rGO) film with abundant interconnected microchannels. This generator enables an around 2-fold open-circuit voltage () and a 3.3-fold power density of 0.91 μW cm in 0.6 M NaCl solution compared to that in DI water. Experiments evidence that the honeycomb structure with abundant interconnected microchannels plays a key role in achieving high and stable output power in salt solution because of its large specific surface area and excellent ion-exchange capacity. Notably, it can work at all times of day and night for more than 240 h in natural seawater, delivering a stable of ∼0.83 V with a power density of 0.79 μW cm. This study expands a working solution for water-evaporation-induced electricity generation from DI water to natural seawater, advancing a great step toward practical applications.
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http://dx.doi.org/10.1021/acsami.1c04508DOI Listing
June 2021

Consumption of Preserved Egg Is Associated with Modestly Increased Risk of Nonalcoholic Fatty Liver Disease in Chinese Adults.

J Nutr 2021 May 28. Epub 2021 May 28.

Nutritional Epidemiology Institute and School of Public Health, Tianjin Medical University, Tianjin, China.

Background: Although preserved egg is a traditional Chinese delicacy widely consumed in China and Southeast Asian countries, whether habitual preserved egg consumption is associated with nonalcoholic fatty liver disease (NAFLD) remains unknown.

Objectives: This study aimed to examine the association between preserved egg consumption and risk of NAFLD in a cohort of Chinese adults.

Methods: This prospective cohort study included 15,883 participants aged 19-88 y (58% women) from the TCLSIH (Tianjin Chronic Low-grade Systemic Inflammation and Health) cohort study who were free of liver diseases, cancer, and cardiovascular disease at baseline. Preserved egg consumption was assessed using an FFQ at baseline. NAFLD was diagnosed by transabdominal sonography during an annual health examination. Multivariable Cox proportional hazards regression models were used to calculate HRs and 95% CIs across categories of preserved egg consumption.

Results: During 56,002 person-years of follow-up, 3683 first incident cases of NAFLD were recorded. After adjustment for sociodemographic characteristics, lifestyle risk factors, total energy intake, egg intake, and eating patterns, the multivariable HRs (95% CIs) of incident NAFLD according to categories of preserved egg consumption were 1.00 (reference) for never, 1.05 (0.98, 1.14) for <1 time/wk, 1.09 (0.96, 1.24) for 1 time/wk, and 1.26 (1.09, 1.46) for ≥2 times/wk (P-trend < 0.01). The results were robust to a series of sensitivity analyses.

Conclusions: Habitual preserved egg consumption is associated with a modestly higher risk of NAFLD among the Chinese adult population. The mechanism underlying this association warrants further research.This trial was registered at www.umin.ac.jp/ctr/ as UMIN000027174.
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http://dx.doi.org/10.1093/jn/nxab163DOI Listing
May 2021

The unique structure of the zebrafish TNF-α homotrimer.

Dev Comp Immunol 2021 Sep 11;122:104129. Epub 2021 May 11.

Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China. Electronic address:

In the current study, zebrafish TNF-α1 (zTNF-α1) was crystallized, and the structure was analyzed. The zTNF-α1 trimer is composed of three monomers whose height and width are 50 Å and 60 Å, respectively. Compared with human TNF-α, zTNF-α1 shows only ~30% amino acid identity, the EF loop of each monomer lacks three amino acids, the CD loop is increased by four amino acids, and the AA'' loop is increased by one amino acid. In addition, an A″-β-chain is added to the zTNF-α1 monomer, forming two β-sheet layers with 6:5 β-chains. The top of the trimer is missing three amino acids and the inner coil because the EF loop seals the central hole at the top, forming a unique structure. In conclusion, the results elucidated the structure of the zTNF-α1 trimer, providing immunological knowledge for studying TNF-α function in the zebrafish animal model and structural information for exploring TNF-α family evolution.
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http://dx.doi.org/10.1016/j.dci.2021.104129DOI Listing
September 2021

The consumption of wholegrain is related to depressive symptoms among Chinese adults: a cross-sectional study.

Eur J Clin Nutr 2021 Apr 30. Epub 2021 Apr 30.

Nutritional Epidemiology Institute and School of Public Health, Tianjin Medical University, Tianjin, China.

Background: Wholegrains contribute a range of beneficial nutrients, such as dietary fiber and several minerals and vitamins, that are beneficial to depressive symptoms. However, there are a few studies aimed at exploring whether a wholegrain diet is related to depressive symptoms. We conducted this study to investigate the relationship between wholegrains consumption and depressive symptoms.

Methods: This cross-sectional study included a total of 24,776 (mean age: 39.9 years, age range: 18.1-91.3 years; males, 54.1%) inhabitants living in Tianjin, China. Wholegrains consumption was assessed using a valid self-administered food frequency questionnaire. Depressive symptoms were evaluated using the Chinese version of Zung Self-Rating Depression Scale (SDS) and the cutoff point was set at 45. Multiple logistic regression analysis was used to estimate the relationship between wholegrains consumption and depressive symptoms.

Results: The prevalence of depressive symptoms was 19.1% and 22.4% in males and females, respectively. After adjustments for potential confounding factors, the odds ratios (95% confidence intervals) of depressive symptoms across wholegrains consumption were 0.77 (0.65-0.91) for <1 time/week, 0.73 (0.62-0.86) for 1 time/week and 0.68 (0.59-0.79) for ≥2 time/week in males compared with the control group (almost never). In females, the odds ratios (95% confidence intervals) were 0.86 (0.71-1.04) for <1 time/week, 0.94 (0.78-1.13) for 1 time/week, and 0.76 (0.65-0.91) for ≥2 time/week. Similar results were observed when we use other cut-offs (SDS ≥ 40 and 50) to define depressive symptoms.

Conclusion: This study first demonstrated that the higher consumption of wholegrains might have effects on the prevention and improvement of depressive symptoms. Prospective or intervention studies are needed to confirm these findings.
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http://dx.doi.org/10.1038/s41430-021-00917-2DOI Listing
April 2021

Systemic Expression, Purification, and Initial Structural Characterization of Bacteriophage T4 Proteins Without Known Structure Homologs.

Front Microbiol 2021 13;12:674415. Epub 2021 Apr 13.

BioBank, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Bacteriophage T4 of is one of the most studied phages. Research into it has led to numerous contributions to phage biology and biochemistry. Coding about 300 gene products, this double-stranded DNA virus is the best-understood model in phage study and modern genomics and proteomics. Ranging from viral RNA polymerase, commonly found in phages, to thymidylate synthase, whose mRNA requires eukaryotic-like self-splicing, its gene products provide a pool of fine examples for phage research. However, there are still up to 130 gene products that remain poorly characterized despite being one of the most-studied model phages. With the recent advancement of cryo-electron microscopy, we have a glimpse of the virion and the structural proteins that present in the final assembly. Unfortunately, proteins participating in other stages of phage development are absent. Here, we report our systemic analysis on 22 of these structurally uncharacterized proteins, of which none has a known homologous structure due to the low sequence homology to published structures and does not belong to the category of viral structural protein. Using NMR spectroscopy and cryo-EM, we provided a set of preliminary structural information for some of these proteins including NMR backbone assignment for Cef. Our findings pave the way for structural determination for the phage proteins, whose sequences are mainly conserved among phages. While this work provides the foundation for structural determinations of proteins like Gp57B, Cef, Y04L, and Mrh, other studies would also benefit from the high yield expression of these proteins.
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http://dx.doi.org/10.3389/fmicb.2021.674415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076793PMC
April 2021

Change in eating habits and physical activities before and during the COVID-19 pandemic in Hong Kong: a cross-sectional study via random telephone survey.

J Int Soc Sports Nutr 2021 Apr 28;18(1):33. Epub 2021 Apr 28.

School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, Hong Kong, China.

Background: Hong Kong is a densely populated city with a low incidence and mortality of coronavirus disease 2019 (COVID-19). The city imposed different levels of social distancing including, the closure of sports venues and restrictions on eateries. This inevitably affects the eating behaviour and physical activities of the population. We examined the changes in eating behavior and physical activities before and during the COVID-19 pandemic, and identified sociodemographic factors associated with the behavioral changes.

Methods: This was a cross-sectional study via a random telephone survey of Chinese adults conducted in Hong Kong from May to June, 2020 - a period in which social distancing measures were being imposed. We measured the physical activity habits from four aspects and dietary consumption patterns from seven aspects before and during the pandemic based on the World Health Organization's guidelines and previous publications.

Results: In total, 724 participants were recruited. Individuals were found to cook more frequently at home (p < 0.001) and order take-out (p < 0.001) during the COVID-19 pandemic. While no significant change in the frequency of fast food consumption was observed, we found significant increases in the frequency of eating fruits (p < 0.001) and vegetables (p = 0.004). The frequencies of walking, moderate-intensive sports, and high-intensity sports were significantly reduced (p < 0.001). We found that healthy lifestyle behaviors during the pandemic were negatively associated with participants' economic status.

Conclusions: Social distancing measures likely provided an opportunity for individuals to stay home and thus eat healthier. However, in a prolonged period of social restrictions, a lower physical activity level poses a risk to public health. Public health officials are thus advised to monitor physical health on a population-wide basis. The findings highlighted the importance of interventions tailored to individuals who have prolonged home stays - particularly for individuals in the low economic group.
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http://dx.doi.org/10.1186/s12970-021-00431-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080997PMC
April 2021

Does a high intake of green leafy vegetables protect from NAFLD? Evidence from a large population study.

Nutr Metab Cardiovasc Dis 2021 06 2;31(6):1691-1701. Epub 2021 Feb 2.

Nutritional Epidemiology Institute and School of Public Health, Tianjin Medical University, Tianjin, China; Health Management Centre, Tianjin Medical University General Hospital, Tianjin, China; Tianjin Key Laboratory of Environment, Nutrition and Public Health, Tianjin, China; Center for International Collaborative Research on Environment, Nutrition and Public Health, Tianjin, China. Electronic address:

Background And Aims: Results of in vitro and in vivo studies showed that green leafy vegetables (GLV) could attenuate liver steatosis. However, little is known regarding the association between GLV intake and nonalcoholic fatty liver disease (NAFLD) in human. We examined the association of GLV intake with NAFLD in a large-scale adult population.

Methods And Results: This cross-sectional study investigated 26,891 adults in China who participated in health examinations from 2013 to 2017. Newly diagnosed NAFLD was detected by liver ultrasonography. Dietary intake was assessed by using a validated and standardized food frequency questionnaire. Multivariable logistic regression models were used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) across categories of GLV intake. After adjustment for sociodemographic characteristics, lifestyle factors, and other dietary intakes, the OR (95% CI) for comparing the highest vs. lowest GLV intake categories (≥7 times/week vs. almost never) was 0.72 (0.59, 0.90) (P < 0.0001). In addition, a linear inverse association was demonstrated between GLV intake and NAFLD in women (P for trend = 0.04), but ORs for any intake category did not reach significance. Stratified analyses suggested a potential effect modification by obesity status; the ORs (95% CIs) for comparing the highest vs. lowest GLV intake categories was 0.72 (0.54, 0.97) in normal/overweight individuals and 1.04 (0.65, 1.65) in obese individuals (P-interaction < 0.0001).

Conclusion: This large population-based study shows that high GLV intake is inversely associated with NAFLD, particularly in women and non-obese participants.
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http://dx.doi.org/10.1016/j.numecd.2021.01.009DOI Listing
June 2021

Higher plain water intake is related to lower newly diagnosed nonalcoholic fatty liver disease risk: a population-based study.

Eur J Clin Nutr 2021 Apr 9. Epub 2021 Apr 9.

Health Management Centre, Tianjin Medical University General Hospital, Tianjin, China.

Background/objectives: High plain water intake (PWI) lowered body weight, reduced total energy intake, and increased fat oxidation and energy consumption. Because such factors are closely linked to metabolic disorders, which are the main risk factors for nonalcoholic fatty liver disease (NAFLD) onset, it was speculated that higher PWI was associated with a lower risk of NAFLD. However, no prior human studies have examined such relationship. Therefore, the purpose of this study was to evaluate the relationship between PWI and newly diagnosed NAFLD in a large-scale adult population.

Subjects/methods: A total of 16,434 participants from 2010 to 2019 in Tianjin, China, were included in this cross-sectional study. PWI was assessed by using a validated self-administered food frequency questionnaire, and it was categorized into three subgroups for analysis: ≤3 cups/day, 4-7 cups/day, and >7 cups/day. NAFLD was diagnosed by abdominal ultrasound. Logistic regression analysis was used to calculate odds ratios (ORs) and 95% confidence intervals (CIs) for the relationship between PWI and NAFLD.

Results: Among 16,434 participants, 20.5% (3,364) had newly diagnosed NAFLD. After controlling for demographic characteristics, lifestyle risk factors, and dietary intake, the adjusted ORs (95% CIs) for having NAFLD across PWI categories were 1.00 (reference) for ≤3 cups/day, 0.84 (0.72, 0.97) for 4-7 cups/day, and 0.77 (0.63, 0.94) for >7 cups/day in males and 1.00 (reference) for ≤3 cups/day, 1.02 (0.81, 1.27) for 4-7 cups/day, and 1.08 (0.78, 1.49) for >7 cups/day in females, respectively.

Conclusions: This study is the first to show that higher PWI is independently related to lower newly diagnosed NAFLD among males, but not females. Further studies are needed to explore the causal relationship.
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http://dx.doi.org/10.1038/s41430-021-00891-9DOI Listing
April 2021

Consumption of chilies and sweet peppers is associated with lower risk of sarcopenia in older adults.

Aging (Albany NY) 2021 03 26;13(6):9135-9142. Epub 2021 Mar 26.

Nutritional Epidemiology Institute and School of Public Health, Tianjin Medical University, Tianjin, China.

Background: Sarcopenia is an aging-related loss of muscle mass and function, which induces numerous adverse outcomes. Capsaicin and capsiate, separately extracted from chilies and sweet peppers, have the potential to induce muscle hypertrophy via activation of transient receptor potential vanilloid 1. The present study aimed to investigate whether chili and sweet pepper consumption are related to sarcopenia in the elderly general population.

Methods: A cross-sectional study with 2,451 participants was performed. Dietary chili and sweet pepper consumption were assessed using a validated self-administered food frequency questionnaire. Sarcopenia was defined according to the consensus of the Asian Working Group for Sarcopenia. Logistic regressions were performed to measure the effect of chili and sweet pepper consumption on sarcopenia.

Results: The prevalence of sarcopenia was 16.1%. After adjustment for potential confounding variables, the odds ratios (95% confidence intervals) for sarcopenia across chili and sweet pepper consumption categories were 1.00 (reference) for almost never, 0.73 (0.55, 0.97) and 0.73 (0.56, 0.96) for ≤1 time/week, 0.60 (0.39, 0.90) and 0.66 (0.45, 0.95) for ≥2-3 times/week (both for trend <0.01), respectively.

Conclusion: The present study showed that higher consumption of chilies and sweet peppers was related to a lower risk of sarcopenia in older adults.
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http://dx.doi.org/10.18632/aging.104168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034967PMC
March 2021

Relationship between seaweeds consumption and hyperuricaemia in general adults: a Population-based study from the Tianjin Chronic Low-grade Systemic Inflammation and Health (TCLSIH) cohort study.

Br J Nutr 2021 Mar 15:1-8. Epub 2021 Mar 15.

Nutritional Epidemiology Institute and School of Public Health, Tianjin Medical University, 22 Qixiangtai Road, Heping District, Tianjin300070, People's Republic of China.

Seaweeds have numerous biologically active ingredients, such as polysaccharides, polyphenols and carotenoids, that are beneficial to human health. Although these benefits might be related to the synthesis, secretion or reabsorption of uric acid, no studies have explored the relationship between seaweeds consumption and hyperuricaemia (HUA) in the general population. The aim of this study was to investigate whether seaweeds consumption is related to HUA in a large-scale adult population. A cross-sectional study was conducted with 32 365 adults (17 328 men and 15 037 women) in Tianjin, People's Republic of China. Frequency of seaweeds consumption was assessed by a validated self-administered FFQ. HUA was defined as serum uric acid levels >420 μmol/L in men and >350 μmol/L in women. The association between seaweeds consumption and HUA was assessed by multiple logistic regression analysis. Restricted cubic spline functions were used for non-linearity tests. The prevalence of HUA in men and women was 21·17 % and 5·93 %, respectively. After adjustments for potential confounding factors, the OR (95 % CI) for HUA across seaweed consumption (g/1000 kcal per d) were 1·00 (reference) for level 1, 0·91 (95 % CI 0·81, 1·02) for level 2; 0·90 (95 % CI 0·81, 1·01) for level 3; 0·86 (95 % CI 0·78, 0·97) for level 4 in men and 0·90 (95 % CI 0·73, 1·10) for level 2; 0·82 (95 % CI 0·67, 1·00) for level 3; 0·84 (95 % CI 0·68, 1·03) for level 4 in women, respectively. A negative correlation between seaweeds consumption and HUA in males but not in females was observed. Further studies are needed to explore the causal relationship.
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http://dx.doi.org/10.1017/S0007114521000891DOI Listing
March 2021

Rescue Protocol to Improve the Image Quality of 18F-FDG PET/CT Myocardial Metabolic Imaging.

Clin Nucl Med 2021 May;46(5):369-374

From the Department of Nuclear Medicine, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing.

Purpose: 18F-FDG PET myocardial metabolic imaging is used to estimate myocardial viability. However, poor image quality can affect the accurate quantification of viable myocardium. We assessed the feasibility of a rescue protocol that reinjected low-dose 18F-FDG with simultaneous 1 to 2 U of insulin injection and oral administration of 10 g of glucose to improve the image quality of 18F-FDG PET myocardial metabolic imaging.

Patients And Methods: Fifty-one consecutive patients with poor quality to uninterpretable 18F-FDG PET/CT myocardial metabolic images received the rescue protocol immediately after the initial image acquisition. The postrescue image acquisition was performed 1 hour later. The rescue image quality was compared with the initial image. The qualitative visual estimation of the images was graded as follows: grade 0, homogeneous, minimal uptake; grade 1, predominantly minimal or mild uptake; grade 2, moderate uptake; and grade 3, good uptake. The myocardium-to-blood pool activity ratio (M/B) was measured to assess the image quality quantitatively.

Results: The grades of 0 to 3 were observed in 24 (47%), 27 (53%), 0 (0%), and 0 (0%) patients, respectively, for the initial imaging, and in 0 (0%), 3 (5.9%), 4 (7.8%), and 44 (86.3%) patients for the rescue imaging (P < 0.001). The rescue M/B was significantly higher than the initial M/B (3.4 ± 1.4 vs 1.6 ± 0.6, respectively; P < 0.001).

Conclusions: The rescue protocol successfully and rapidly improved the quality of myocardial 18F-FDG metabolic imaging.
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http://dx.doi.org/10.1097/RLU.0000000000003572DOI Listing
May 2021

The Crystal Structure of the MHC Class I (MHC-I) Molecule in the Green Anole Lizard Demonstrates the Unique MHC-I System in Reptiles.

J Immunol 2021 Apr 26;206(7):1653-1667. Epub 2021 Feb 26.

Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China

The reptile MHC class I (MCH-I) and MHC class II proteins are the key molecules in the immune system; however, their structure has not been investigated. The crystal structure of green anole lizard peptide-MHC-I-β2m (pMHC-I or p-UA*0101) was determined in the current study. Subsequently, the features of p-UA*0101 were analyzed and compared with the characteristics of pMHC-I of four classes of vertebrates. The amino acid sequence identities between -UA*0101 and MHC-I from other species are <50%; however, the differences between the species were reflected in the topological structure. Significant characteristics of p-UA*0101 include a specific flip of ∼88° and an upward shift adjacent to the C terminus of the α1- and α2-helical regions, respectively. Additionally, the lizard MHC-I molecule has an insertion of 2 aa (VE) at positions 55 and 56. The pushing force from 55-56VE triggers the flip of the α1 helix. Mutagenesis experiments confirmed that the 55-56VE insertion in the α1 helix enhances the stability of p-UA*0101. The peptide presentation profile and motif of p-UA*0101 were confirmed. Based on these results, the proteins of three reptile lizard viruses were used for the screening and confirmation of the candidate epitopes. These data enhance our understanding of the systematic differences between five classes of vertebrates at the gene and protein levels, the formation of the pMHC-I complex, and the evolution of the MHC-I system.
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http://dx.doi.org/10.4049/jimmunol.2000992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7980529PMC
April 2021

Milk fat globule EGF factor 8 restores mitochondrial function via integrin-medicated activation of the FAK-STAT3 signaling pathway in acute pancreatitis.

Clin Transl Med 2021 02;11(2):e295

National Local Joint Engineering Research Center for Precision Surgery & Regenerative Medicine, Shaanxi Provincial Center for Regenerative Medicine and Surgical Engineering, First Affiliated Hospital of Xi'an Jiaotong University., Xi'an, Shaanxi Province, China.

Acute pancreatitis (AP) remains a significant clinical challenge. Mitochondrial dysfunction contributes significantly to the pathogenesis of AP. Milk fat globule EGF factor 8 (MFG-E8) is an opsonizing protein, which has many biological functions via binding to αvβ3/5 integrins. Ligand-dependent integrin-FAK activation of STAT3 was reported to be of great importance for maintaining a normal mitochondrial function. However, MFG-E8's role in AP has not been evaluated.

Methods: Blood samples were acquired from 69 healthy controls and 134 AP patients. Serum MFG-E8 levels were measured by ELISA. The relationship between serum concentrations of MFG-E8 and disease severity were analyzed. The role of MFG-E8 was evaluated in experimental models of AP.

Results: Serum concentrations of MFG-E8 were lower in AP patients than healthy controls. And serum MFG-E8 concentrations were negatively correlated with disease severity in AP patients. In mice, MFG-E8 administration decreased L-arginine-induced pancreatic injury and mortality. MFG-E8's protective effects in experimental AP were associated with improvement in mitochondrial function and reduction in oxidative stress. MFG-E8 knockout mice suffered more severe pancreatic injury and greater mitochondrial damage after l-arginine administration. Mechanistically, MFG-E8 activated the FAK-STAT3 pathway in AP mice. Cilengitide, a specific αvβ3/5 integrin inhibitor, abolished MFG-E8's beneficial effects in AP. PF00562271, a specific FAK inhibitor, blocked MFG-E8-induced STAT3 phosphorylation. APTSTAT3-9R, a specific STAT3 antagonist, also eliminated MFG-E8's beneficial effects under such a condition.

Conclusions: MFG-E8 acts as an endogenous protective mediator in the pathogenesis of AP. MFG-E8 administration protects against AP possibly by restoring mitochondrial function via activation of the integrin-FAK-STAT3 signaling pathway. Targeting the action of MFG-E8 may present a potential therapeutic option for AP.
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http://dx.doi.org/10.1002/ctm2.295DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828261PMC
February 2021

Limited role for meteorological factors on the variability in COVID-19 incidence: A retrospective study of 102 Chinese cities.

PLoS Negl Trop Dis 2021 02 24;15(2):e0009056. Epub 2021 Feb 24.

Jockey Club School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China.

While many studies have focused on identifying the association between meteorological factors and the activity of COVID-19, we argue that the contribution of meteorological factors to a reduction of the risk of COVID-19 was minimal when the effects of control measures were taken into account. In this study, we assessed how much variability in COVID-19 activity is attributable to city-level socio-demographic characteristics, meteorological factors, and the control measures imposed. We obtained the daily incidence of COVID-19, city-level characteristics, and meteorological data from a total of 102 cities situated in 27 provinces/municipalities outside Hubei province in China from 1 January 2020 to 8 March 2020, which largely covers almost the first wave of the epidemic. Generalized linear mixed effect models were employed to examine the variance in the incidence of COVID-19 explained by different combinations of variables. According to the results, including the control measure effects in a model substantially raised the explained variance to 45%, which increased by >40% compared to the null model that did not include any covariates. On top of that, including temperature and relative humidity in the model could only result in < 1% increase in the explained variance even though the meteorological factors showed a statistically significant association with the incidence rate of COVID-19. In conclusion, we showed that very limited variability of the COVID-19 incidence was attributable to meteorological factors. Instead, the control measures could explain a larger proportion of variance.
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http://dx.doi.org/10.1371/journal.pntd.0009056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904227PMC
February 2021

Mink SLAM V-Region V74I Substitutions Contribute to the Formation of Syncytia Induced by Canine Distemper Virus.

Front Vet Sci 2020 21;7:570283. Epub 2021 Jan 21.

College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China.

The Signal lymphatic activation molecule (SLAM, also known as CD150) as the cellular receptor of canine distemper virus (CDV) plays an important role in the virus-host interaction. However, it is still unknown whether amino acid differences in the SLAM variable (V) region affect the formation of syncytia. Here, using raccoon dog SLAM (rSLAM) and mink SLAM (mSLAM), we performed SLAM-V homologous modeling, site-directed mutagenesis, and surface expression analysis, as well as a cell fusion assay, to study the interaction between SLAM and CDV. More specifically, our investigation focused on two amino acid residues (74 and 129) of SLAM, previously predicted to play a relevant role in receptor-ligand interaction. Our results indicated that only residues at position 60, 74, and 129 were different between rSLAM and mSLAM among the 29 amino acids that might interact with CDV H, and residues 74 and 129 were located in the interface region interacting with CDV H. The amino acid substitution at the positions of 74 have a significant effect on the expression of mSLAM. The SLAM-V74I mutation in mink significantly improved the cell fusion efficiency of CDV. In contrast, the SLAM-I74V mutation in the raccoon dog significantly decreased cell fusion efficiency. We conclude that residue 74 of SLAM plays an important role during the the formation of syncytia. Only when implementing CDV infection analysis, the rSLAM-Q129R can significantly decreased the mean number of syncytia, but the mSLAM-R129Q can't. Additionally, residue 60 show variability between rSLAM and mSLAM. We believe that our study makes a significant contribution to the literature because we provide molecular data, partially accounting for the differences in host membrane and virus interaction laying the foundation for further molecular work.
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http://dx.doi.org/10.3389/fvets.2020.570283DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874165PMC
January 2021

Soft drink consumption and risk of nonalcoholic fatty liver disease: results from the Tianjin Chronic Low-Grade Systemic Inflammation and Health (TCLSIH) cohort study.

Am J Clin Nutr 2021 05;113(5):1265-1274

Nutritional Epidemiology Institute and School of Public Health, Tianjin Medical University, Tianjin, China.

Background: Epidemiological evidence for the association of soft drink consumption with nonalcoholic fatty liver disease (NAFLD) is inconsistent, and such association has not been prospectively examined in the general adult population.

Objectives: We aimed to investigate the prospective association between soft drink consumption and the risk of NAFLD in a Chinese adult population.

Methods: This prospective cohort study investigated 14,845 participants [mean age: 39.3 y; 6203 (41.8%) men] who were free of liver disease, cardiovascular disease, and cancer at baseline. Soft drink consumption (mainly sugar-containing carbonated beverages) was measured at baseline using a validated FFQ. NAFLD was diagnosed based on abdominal ultrasound without significant alcohol consumption and other causes of liver disease. Hepatic steatosis index (HSI) was calculated based on sex, BMI, and blood transaminase levels. Cox proportional hazards regression models were used to examine the association of soft drink consumption with incident NAFLD.

Results: A total of 2888 first-incident cases of NAFLD occurred during 42,048 person-years of follow-up (median follow-up: 4.2 y). After adjusting for demographic characteristics, lifestyle factors, dietary intake, and inflammatory markers, the multivariable HRs (95% CIs) for incident NAFLD were 1.00 (reference) for <1 serving/wk, 1.18 (1.03, 1.34) for 1 serving/wk, 1.23 (1.08, 1.40) for 2-3 servings/wk, and 1.47 (1.25, 1.73) for ≥4 servings/wk, respectively (P for trend < 0.0001). Further sensitivity analysis showed that the corresponding multivariable HRs (95% CIs) for incident HSI-defined NAFLD were 1.00 (reference), 0.96 (0.70, 1.31), 1.16 (0.83, 1.62), and 1.59 (1.07, 2.37), respectively (P for trend < 0.0001).

Conclusions: The results from our prospective study indicate that soft drink consumption is associated with an increased risk of NAFLD in Chinese adults. This study was registered at UMIN Clinical Trials Registry as UMIN000027174.
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http://dx.doi.org/10.1093/ajcn/nqaa380DOI Listing
May 2021

High-normal thyroid function predicts incident non-alcoholic fatty liver disease among middle-aged and elderly euthyroid subjects.

J Gerontol A Biol Sci Med Sci 2021 Feb 3. Epub 2021 Feb 3.

Nutritional Epidemiology Institute and School of Public Health, Tianjin Medical University, Tianjin, China.

Background: Thyroid hormones (THs) influence hepatic lipid homeostasis through multiple pathways, suggesting that THs may predict the risk of non-alcoholic fatty liver disease (NAFLD). However, prospective studies on the association between THs levels and incident NAFLD in euthyroid subjects are limited. This prospective cohort study aimed to explore whether THs were associated with the development of NAFLD in middle-aged and elderly euthyroid subjects.

Methods: A total of 6,462 subjects without baseline NAFLD were included in the cohort study (~6-year follow-up period, median: 4.2 years). Chemiluminescence immunoassay was used to measure serum free triiodothyronine (FT3), free thyroxine (FT4), and thyroid-stimulating hormone (TSH), and NAFLD was diagnosed by abdominal ultrasonography. Multivariable Cox proportional hazards regression models were used to assess the association between baseline THs, TSH, and the risk of NAFLD.

Results: During the follow-up period, 1,675 subjects developed NAFLD. The incidence rate of NAFLD was 85.0 per 1000 person-years. Compared with the lowest FT3, FT4, and TSH quartiles, the multivariable-adjusted hazard ratios (95% confidence interval) of incident NAFLD for highest quartiles were 1.30 (1.12, 1.51), 1.07 (0.93, 1.23), 0.82 (0.71, 0.95) (P <0.001, =0.56, =0.01, respectively), respectively.

Conclusions: In middle-aged and elderly euthyroid subjects, high-normal FT3 and low-normal TSH are independently associated with a higher incidence of NAFLD.
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http://dx.doi.org/10.1093/gerona/glab037DOI Listing
February 2021

Predictive Value of Thyroid Hormones for Incident Hyperuricemia in Euthyroid Subjects: The Tianjin Chronic Low-Grade Systemic Inflammation and Health Cohort Study.

Endocr Pract 2021 Apr 30;27(4):291-297. Epub 2021 Jan 30.

Nutritional Epidemiology Institute and School of Public Health, Tianjin Medical University, Tianjin, China; Health Management Centre, Tianjin Medical University General Hospital, Tianjin, China; Tianjin Key Laboratory of Environment, Nutrition and Public Health, Tianjin, China; Center for International Collaborative Research on Environment, Nutrition and Public Health, Tianjin, China. Electronic address:

Objective: Thyroid hormones (THs) play an important role in both serum uric acid (SUA) excretion and purine nucleotide metabolism. Past research mainly focused on the relationships between thyroid dysfunction and hyperuricemia. Although most subjects at risk for hyperuricemia are euthyroid, few studies have investigated the predictive values of THs on incident hyperuricemia in euthyroid adults. This study aimed to examine how free triiodothyronine, thyroxine, and thyroid-stimulating hormone are related to incident hyperuricemia in euthyroid subjects.

Methods: Participants without baseline hyperuricemia were recruited from Tianjin Chronic Low-grade Systemic Inflammation and Health Cohort Study and followed up for ∼6 years. Thyroid function was determined by chemiluminescence immunoassay methods. Hyperuricemia was defined as SUA ≥416.5 μmol/L for males and ≥357.0 μmol/L for females. Thyroid function and SUA were assessed yearly during follow-up. Adjusted Cox proportional hazards regression models were used to assess the relationship between thyroid function and hyperuricemia.

Results: The incidence rates of hyperuricemia were 109 and 50 per 1000 person-years in males and females, respectively. In males, compared with the lowest quartile, the multivariable-adjusted hazards ratios for hyperuricemia in the highest quartiles of triiodothyronine, thyroxine, and thyroid-stimulating hormone were 0.57 (0.50-0.66), 0.63 (0.54-0.73), and 1.03 (0.90-1.19) (P for trend < .0001, < .0001, and .51), respectively. However, no statistically significant correlations between thyroid function and incident hyperuricemia in females were found.

Conclusion: This cohort study is the first to demonstrate that higher THs are related to lower risk of incident hyperuricemia in a male population with euthyroid status.
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http://dx.doi.org/10.1016/j.eprac.2020.10.009DOI Listing
April 2021
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