Yashas Rajendra

Yashas Rajendra

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Yashas Rajendra

Yashas Rajendra

Publications by authors named "Yashas Rajendra"

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PEI-Mediated Transient Gene Expression in CHO Cells.

Authors:
Yashas Rajendra

Methods Mol Biol 2018 ;1850:33-42

Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

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http://link.springer.com/10.1007/978-1-4939-8730-6_3
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http://dx.doi.org/10.1007/978-1-4939-8730-6_3DOI Listing
May 2019

Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension.

Methods Mol Biol 2017 ;1603:45-55

Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.

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http://dx.doi.org/10.1007/978-1-4939-6972-2_3DOI Listing
February 2018

Evaluation of piggyBac-mediated CHO pools to enable material generation to support GLP toxicology studies.

Biotechnol Prog 2017 11 29;33(6):1436-1448. Epub 2017 Jun 29.

Biotechnology Discovery Research, Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN, 46225.

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http://doi.wiley.com/10.1002/btpr.2495
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http://dx.doi.org/10.1002/btpr.2495DOI Listing
November 2017

Polymer-mediated flocculation of transient CHO cultures as a simple, high throughput method to facilitate antibody discovery.

Biotechnol Prog 2017 Sep 7;33(5):1393-1400. Epub 2017 Aug 7.

Biotechnology Discovery Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285.

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http://dx.doi.org/10.1002/btpr.2527DOI Listing
September 2017

Transient and stable CHO expression, purification and characterization of novel hetero-dimeric bispecific IgG antibodies.

Biotechnol Prog 2017 03 3;33(2):469-477. Epub 2017 Feb 3.

Biotechnology Discovery Research, Lilly Biotechnology Center, San Diego, CA, 92121.

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http://dx.doi.org/10.1002/btpr.2414DOI Listing
March 2017

Bioreactor scale up and protein product quality characterization of piggyBac transposon derived CHO pools.

Biotechnol Prog 2017 03 7;33(2):534-540. Epub 2017 Mar 7.

Biotechnology Discovery Research, Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN, 46225.

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http://dx.doi.org/10.1002/btpr.2447DOI Listing
March 2017

Enhanced plasmid DNA utilization in transiently transfected CHO-DG44 cells in the presence of polar solvents.

Biotechnol Prog 2015 Nov-Dec;31(6):1571-8. Epub 2015 Aug 25.

Laboratory for Cellular Biotechnology (LBTC) and Protein Expression Core Facility (PECF), École Polytechnique Fédérale De Lausanne (EPFL), Lausanne, 1015, Switzerland.

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http://dx.doi.org/10.1002/btpr.2152DOI Listing
October 2016

Generation of stable Chinese hamster ovary pools yielding antibody titers of up to 7.6 g/L using the piggyBac transposon system.

Biotechnol Prog 2016 09 29;32(5):1301-1307. Epub 2016 Jun 29.

Biotechnology Discovery Research, Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN, 46285.

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http://doi.wiley.com/10.1002/btpr.2307
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http://dx.doi.org/10.1002/btpr.2307DOI Listing
September 2016

Comparison of three transposons for the generation of highly productive recombinant CHO cell pools and cell lines.

Biotechnol Bioeng 2016 06 9;113(6):1234-43. Epub 2015 Dec 9.

Laboratory of Cellular Biotechnology, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1002/bit.25888DOI Listing
June 2016

Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells.

Biotechnol Prog 2015 Mar-Apr;31(2):541-9. Epub 2015 Feb 26.

Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.

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http://dx.doi.org/10.1002/btpr.2064DOI Listing
January 2016

High-throughput mAb expression and purification platform based on transient CHO.

Biotechnol Prog 2015 Jan-Feb;31(1):239-47. Epub 2014 Nov 28.

Biotechnology Discovery Research, Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN, 46285.

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http://dx.doi.org/10.1002/btpr.2012DOI Listing
December 2015

Transcriptional and post-transcriptional targeting for enhanced transient gene expression in CHO cells.

Biotechnol Lett 2015 Dec 23;37(12):2379-86. Epub 2015 Aug 23.

Biotechnology Discovery Research, Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN, 46285, USA.

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http://link.springer.com/content/pdf/10.1007/s10529-015-1938
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http://link.springer.com/10.1007/s10529-015-1938-6
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http://dx.doi.org/10.1007/s10529-015-1938-6DOI Listing
December 2015

A high cell density transient transfection system for therapeutic protein expression based on a CHO GS-knockout cell line: process development and product quality assessment.

Biotechnol Bioeng 2015 May 16;112(5):977-86. Epub 2015 Mar 16.

Biotechnology Discovery Research, Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, 46285, Indiana.

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http://dx.doi.org/10.1002/bit.25514DOI Listing
May 2015

Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells.

BMC Proc 2011 22;5 Suppl 8:P35. Epub 2011 Nov 22.

Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédéral de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1186/1753-6561-5-S8-P35DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284955PMC
April 2015

Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin(®) bioreactors.

BMC Proc 2011 22;5 Suppl 8:P38. Epub 2011 Nov 22.

Laboratory of Cellular Biotechnology, Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1186/1753-6561-5-S8-P38DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3284980PMC
April 2015

The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells.

BMC Proc 2011 22;5 Suppl 8:P33. Epub 2011 Nov 22.

Laboratory for Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédéral de Lausanne, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1186/1753-6561-5-S8-P33DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285026PMC
April 2015

Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells.

Int J Mol Cell Med 2014 ;3(3):146-56

Laboratory of Cellular Biotechnology, Ècole Polytechnique Fédérale de Lausanne, Switzerland.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4170488PMC
October 2014

Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells.

Protein Expr Purif 2013 Nov 8;92(1):67-76. Epub 2013 Sep 8.

Protein Expression Core Facility, EPFL, CH-1015 Lausanne, Switzerland; Laboratory of Cellular Biotechnology, EPFL, CH-1015 Lausanne, Switzerland. Electronic address:

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http://dx.doi.org/10.1016/j.pep.2013.09.001DOI Listing
November 2013

DNA delivery with hyperbranched polylysine: a comparative study with linear and dendritic polylysine.

J Control Release 2013 Aug 1;169(3):276-88. Epub 2013 Feb 1.

Ecole Polytechnique Fédérale de Lausanne (EPFL), Institut des Matériaux et Institut des Sciences et Ingénierie Chimiques, Laboratoire des Polymères, Bâtiment MXD, Station 12, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1016/j.jconrel.2013.01.019DOI Listing
August 2013

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells.

Biotechnol Bioeng 2012 Sep 30;109(9):2271-8. Epub 2012 Mar 30.

Laboratory for Cellular Biotechnology, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

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http://dx.doi.org/10.1002/bit.24494DOI Listing
September 2012

Hyperbranched polylysine: a versatile, biodegradable transfection agent for the production of recombinant proteins by transient gene expression and the transfection of primary cells.

Macromol Biosci 2012 Jun 11;12(6):794-804. Epub 2012 Apr 11.

Institut des Matériaux et Institut des Sciences et Ingénierie Chimiques, École Polytechnique Fédérale de Lausanne-EPFL, Bâtiment MXD, Station 12, CH-1015 Lausanne, Switzerland.

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http://dx.doi.org/10.1002/mabi.201100519DOI Listing
June 2012

Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells.

Biotechnol Lett 2012 Apr 30;34(4):619-26. Epub 2011 Nov 30.

Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédéral de Lausanne (EPFL), 1015, Lausanne, Switzerland.

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http://dx.doi.org/10.1007/s10529-011-0809-zDOI Listing
April 2012

A simple high-yielding process for transient gene expression in CHO cells.

J Biotechnol 2011 Apr 15;153(1-2):22-6. Epub 2011 Mar 15.

Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédéral de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

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http://ac.els-cdn.com/S016816561100112X/1-s2.0-S016816561100
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http://linkinghub.elsevier.com/retrieve/pii/S016816561100112
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http://dx.doi.org/10.1016/j.jbiotec.2011.03.001DOI Listing
April 2011

Molecular epidemiology of metallo-beta-lactamase-producing Pseudomonas aeruginosa isolates from Norway and Sweden shows import of international clones and local clonal expansion.

Antimicrob Agents Chemother 2010 Jan 2;54(1):346-52. Epub 2009 Nov 2.

Reference Centre for Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, N-9038 Tromsø, Norway.

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http://dx.doi.org/10.1128/AAC.00824-09DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2798561PMC
January 2010