Publications by authors named "Yaqin Zhang"

144 Publications

Glycosylation in Cervical Cancer: New Insights and Clinical Implications.

Front Oncol 2021 16;11:706862. Epub 2021 Aug 16.

Key Laboratory of Medical Science and Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University, Zhenjiang, China.

Cervical cancer has become the most frequent female malignancy and presents as a general health challenge in many countries undergoing economic development. Various human papillomaviruses (HPV) types have appeared as one of the most critically identifiable causes of widespread cervical cancers. Conventional cervical cytological inspection has limitations of variable sensitivity according to cervical cytology. Glycobiology has been fundamental in related exploration in various gynecologic and reproductive fields and has contributed to our understanding of cervical cancer. It is associated with altered expression of N-linked glycan as well as abnormal expression of terminal glycan structures. The analytical approaches available to determine serum and tissue glycosylation, as well as potential underlying molecular mechanisms involved in the cellular glycosylation alterations, are monitored. Moreover, cellular glycosylation influences various aspects of cervical cancer biology, ranging from cell surface expressions, cell-cell adhesion, cancer signaling, cancer diagnosis, and management. In general, discoveries in glycan profiling make it technically reproducible and affordable to perform serum glycoproteomic analyses and build on previous work exploring an expanded variety of glycosylation markers in the majority of cervical cancer patients.
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http://dx.doi.org/10.3389/fonc.2021.706862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415776PMC
August 2021

Calculation of Toric Intraocular Lens Power with the Barrett Calculator and Data from Three Keratometers.

J Trop Med 2021 20;2021:7712345. Epub 2021 Aug 20.

Shanxi Eye Hospital Affiliated to Shanxi Medical University, Taiyuan, Shanxi, China.

Aim: To investigate the interdevice agreement for differences in toric power calculated using data on anterior corneal astigmatism obtained with corneal topography/ray-tracing aberrometry (iTrace), partial coherence interferometry (IOLMaster 500), and Scheimpflug imaging (Pentacam).

Methods: The analysis included 101 eyes (101 subjects) with regular astigmatism. The main outcome measures were corneal cylinder power, axis of astigmatism, and keratometry values. Toricity and toric IOL power were calculated using the online Barrett toric calculator. Interdevice agreement for measurement and calculation was assessed using a paired sample -test and a nonparametric test.

Results: Significant interdevice differences were noted in the magnitude of astigmatism and flat, steep, and mean keratometry values between iTrace and IOLMaster (all < 0.01); in flat, steep, and mean keratometry values (all < 0.001) but not in the magnitude of astigmatism (=0.325) between iTrace and Pentacam; and in the magnitude of astigmatism and steep and mean keratometry values (all < 0.01) but not in flat keratometry values (=0.310) between IOLMaster and Pentacam. The toric IOL power calculated using data from the three devices showed the following trend: iTrace > IOLMaster (0.49 ± 0.36, < 0.001) and Pentacam (0.39 ± 0.42, < 0.001) and Pentacam was
Conclusions: Differences in toric IOL power and toricity calculated using anterior keratometry data from iTrace, IOLMaster 500, and Pentacam should be noted in clinical practice.
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http://dx.doi.org/10.1155/2021/7712345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405298PMC
August 2021

Effects of Exenatide on Coagulation and Platelet Aggregation in Patients with Type 2 Diabetes.

Drug Des Devel Ther 2021 12;15:3027-3040. Epub 2021 Jul 12.

Department of Rheumatology, The First Affiliated Hospital of Anhui Medical University, Hefei, 230022, People's Republic of China.

Objective: To explore the effect of the glucagon-like peptide-1 receptor agonist exenatide on coagulation function and platelet aggregation in patients with type 2 diabetes mellitus (T2DM).

Methods: Thirty patients with newly diagnosed T2DM were enrolled as the case group, and 30 healthy people with matching age and sex were selected as the control group. Patients in the case group received exenatide treatment for 8 weeks. The general clinical data and biochemical indicators of all subjects were collected; and their peripheral blood platelet count, coagulation index, nitric oxide (NO), platelet membrane glycoprotein (CD62p), platelet activation complex-1 (PAC-1) and platelet aggregation induced by collagen, epinephrine (EPI), arachidonic acid (AA), and adenosine diphosphate (ADP) were detected.

Results: The fibrinogen, CD62p, PAC-1, and platelet aggregation rates of the case group (pretreatment) are higher than those in the control group (EPI 77.90±6.31 vs 60.15±5.37, ADP 52.89±9.36 vs 47.90±6.16, and AA 76.09±3.14 vs.55.18±3.55); and the NO level is lower in the case group than in the control group (<0.05, respectively). After 8 weeks of exenatide treatment in the case group, the CD62p, PAC-1, and platelet aggregation rates were lower than before the treatment (EPI: 61.96±8.94 vs 77.90±6.31 and AA: 50.98±6.73 vs 76.09±3.14); and the NO level was higher than before the treatment (<0.05, respectively). Pearson correlation analysis showed that the changes in platelet aggregation rates (Δ EPI and ΔAA) of the patients in the case group after 8 weeks of exenatide treatment were positively correlated with the changes in body mass index, waist circumference, weight, blood lipids, fasting plasma glucose, haemoglobin A1c, fibrinogen, CD62p, and PAC-1 and negatively correlated with the changes in high-density lipoprotein and NO (<0.05). Multiple linear regression analysis showed that the changes in NO, CD62p and PAC-1 were independent risk factors affecting the changes in platelet aggregation rates.

Conclusion: The GLP-1R agonist exenatide can inhibit the activation state of platelets in patients with T2DM and inhibit thrombosis, which is beneficial to reduce the risk of cardiovascular events.
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http://dx.doi.org/10.2147/DDDT.S312347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285923PMC
July 2021

Clinical analysis of 10 cases of pituitary stalk interruption syndrome and literature review.

Neuro Endocrinol Lett 2021 Jul;42(3):150-156

Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China.

Objective: To analyze the clinical characteristics and MRI imaging of the patients with pituitary stalk interruption syndrome (PSIS) in the First Affiliated Hospital of Anhui Medical University in the past four years, and to achieve better comprehension of this disease.

Methods: Ten patients with PSIS (9 males, 1 female) in our hospital were retrospectively analyzed, regarding clinical manifestation, laboratory data and MRI imaging.

Results: The clinical features of 10 cases of PSIS were as follows: growth retardation, 55% of patients with hypogonadism, 45% of patients with short stature; the dystocia rate at birth is as high as 90%, of which 61% are breech presentation and 27% are foot presentation; 10 patients with PSIS, the height was between 135 cm and 180 cm, the weight was between 31 kg and 93 kg, the lower part was larger than the upper part, and the finger distance was smaller than the height; bone age is behind 3~7 years old; normal intelligence; 10 patients have clinical manifestations of hypopituitary hypofunction; no manifestations of diabetes insipidus; MRI imaging of pituitary showed that the pituitary stalks were not shown, atrophy or aplasia of anterior pituitary, posterior pituitary ectopic.

Conclusion: The incidence of PSIS is low, and the etiology and pathogenesis are unknown. Appropriate hormonal replacement therapy is the only effective way but the timing of treatment is very important. Therefore, clinical doctors should strengthen the awareness of the disease, and master the clinical characteristics of the disease to grasp the timing of treatment.
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July 2021

Sensitive and rapid on-site detection of SARS-CoV-2 using a gold nanoparticle-based high-throughput platform coupled with CRISPR/Cas12-assisted RT-LAMP.

Sens Actuators B Chem 2021 Oct 6;345:130411. Epub 2021 Jul 6.

School of Biomedical Engineering, Sun Yat-sen University, Shenzhen, 518107, China.

The outbreak of corona virus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic. The high infectivity of SARS-CoV-2 highlights the need for sensitive, rapid and on-site diagnostic assays of SARS-CoV-2 with high-throughput testing capability for large-scale population screening. The current detection methods in clinical application need to operate in centralized labs. Though some on-site detection methods have been developed, few tests could be performed for high-throughput analysis. We here developed a gold nanoparticle-based visual assay that combines with CRISPR/Cas12a-assisted RT-LAMP, which is called Cas12a-assisted RT-LAMP/AuNP (CLAP) assay for rapid and sensitive detection of SARS-CoV-2. In optimal condition, we could detect down to 4 copies/μL of SARS-CoV-2 RNA in 40 min. by naked eye. The sequence-specific recognition character of CRISPR/Cas12a enables CLAP a superior specificity. More importantly, the CLAP is easy for operation that can be extended to high-throughput test by using a common microplate reader. The CLAP assay holds a great potential to be applied in airports, railway stations, or low-resource settings for screening of suspected people. To the best of our knowledge, this is the first AuNP-based colorimetric assay coupled with Cas12 and RT-LAMP for on-site diagnosis of COVID-19. We expect CLAP assay will improve the current COVID-19 screening efforts, and make contribution for control and mitigation of the pandemic.
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http://dx.doi.org/10.1016/j.snb.2021.130411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257267PMC
October 2021

Tracking the Micro-Heterogeneity and Hydrogen-Bonding Interactions in Hydroxyl-Functionalized Ionic Liquid Solutions: A Combined Experimental and Computational Study.

Chemphyschem 2021 Sep 4;22(18):1891-1899. Epub 2021 Aug 4.

MOE Key Laboratory of Bioorganic Phosphorous Chemistry and Chemical Biology, Department of Chemistry, Tsinghua University, Beijing, 100084, P. R. China.

Ionic liquids (ILs) are an important class of media that are usually used in combination with polar solvents to reduce costs and tune their physicochemical properties. In this regard, it is essential to understand the influence of adding solvents on the properties of ILs. In this work, the micro-heterogeneity and H-bonding interactions between a hydroxyl-functionalized IL, [HOEmim][TFSI], and acetonitrile (ACN) were investigated by attenuated total reflection Fourier transform infrared spectroscopy and molecular simulations. All studied IL-ACN mixtures were found to deviate from the ideal mixtures. The degree of deviations reaches the maximum at about x(ACN)=0.7 with the presence of both homogeneous clusters of pure IL/ACN and heterogeneous clusters of IL-ACN. With the addition of ACN to IL, the mixtures undergo the transformation from "ACN solvated in [HOEmim][TFSI]" to "[HOEmim][TFSI] solvated in ACN". It is found that the newly formed H-bonding interactions between the IL and ACN is the main factor that contributes to the red shifts of O-H, C -H, C -H, and C -H of [HOEmim] cation, and the blue shifts of C-D, C≡N of ACN, and C-F, S=O of [TFSI] anion. These in-depth studies on the mixtures of hydroxyl-functionalized IL and acetonitrile would help to understand the micro-heterogeneity and H-bonding interactions of miscible solutions and shed light on exploring their applications.
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http://dx.doi.org/10.1002/cphc.202100395DOI Listing
September 2021

M1 macrophage-derived exosomes impair beta cell insulin secretion via miR-212-5p by targeting SIRT2 and inhibiting Akt/GSK-3β/β-catenin pathway in mice.

Diabetologia 2021 Sep 11;64(9):2037-2051. Epub 2021 Jun 11.

Key Laboratory of Human Functional Genomics of Jiangsu Province, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, China.

Aims/hypothesis: Macrophage levels are elevated in pancreatic islets, and the resulting inflammatory response is a major contributor to beta cell failure during obesity and type 2 diabetes mellitus. Previous studies by us and others have reported that exosomes released by macrophages play important roles in mediating cell-to-cell communication, and represent a class of inflammatory factors involved in the inflammatory process associated with type 2 diabetes mellitus. However, to date, no reports have demonstrated the effect of macrophage-derived exosomes on beta cells, and little is known regarding their underlying mechanisms in beta cell injury. Thus, we aimed to study the impact of macrophage-derived exosomes on islet beta cell injury in vitro and in vivo.

Methods: The phenotypic profiles of islet-resident macrophages were analysed in C57BL/6J mice fed a high-fat diet (HFD). Exosomes were collected from the medium of cultured bone marrow-derived macrophages (BMDMs) and from isolated islet-resident macrophages of HFD-fed mice (HFD-Exos). The role of exosomes secreted by inflammatory M1 phenotype BMDMs (M1-Exos) and HFD-Exos on beta cell function was assessed. An miRNA microarray and quantitative real-time PCR (qPCR) were conducted to test the level of M1-Exos-derived miR-212-5p in beta cells. Then, miR-212-5p was overexpressed or inhibited in M1-Exos or beta cells to determine its molecular and functional impact.

Results: M1-polarised macrophages were enriched in the islets of obese mice. M1 macrophages and islet-resident macrophages of HFD-fed mice impaired beta cell insulin secretion in an exosome-dependent manner. miR-212-5p was notably upregulated in M1-Exos and HFD-Exos. Enhancing the expression of miR-212-5p impaired beta cell insulin secretion. Blocking miR-212-5p elicited a significant improvement in M1-Exos-mediated beta cell insulin secretion during injury. Mechanistically, M1-Exos mediated an intercellular transfer of the miR-212-5p, targeting the sirtuin 2 gene and regulating the Akt/GSK-3β/β-catenin pathway in recipient beta cells to restrict insulin secretion.

Conclusions/interpretation: A novel exosome-modulated mechanism was delineated for macrophage-beta cell crosstalk that drove beta cell dysfunction and should be explored for its therapeutic utility.
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http://dx.doi.org/10.1007/s00125-021-05489-1DOI Listing
September 2021

The expression and diagnostic value of serum levels of EphA2 and VEGF-A in patients with colorectal cancer.

Cancer Biomark 2021 ;31(4):399-408

Department of General Surgery, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.

Background: Several molecules are highly expressed in the serum of cancer patients, and can be used as serological markers. This approach has become one of the important auxiliary diagnostic methods for cancer.

Aim: To investigate the correlation between the serum levels of EphA2 and VEGF-A and the pathogenesis of colorectal cancer (CRC) as well as the potential value of these molecules in the diagnosis of CRC.

Methods: ELISA was used to detect the levels of EphA2 and VEGF-A in the peripheral venous serum of 106 newly diagnosed patients with CRC and 69 normal controls. The relationship between the serum EphA2 and VEGF-A levels and the clinicopathological characteristics of CRC patients was analyzed. ROC analysis was used to investigate the diagnostic value of the serum EphA2 and VEGF-A levels in CRC, and the optimal cutoff value was calculated.

Results: The serum levels of EphA2 and VEGF-A in the CRC group were higher than those in the control as well as CEA, the serum level of EphA2 was positively correlated with the VEGF-A levels, but neither was significantly associated with the clinicopathological parameters of CRC. The ROC curve showed that the single index AUC was < 0.7 except for VEGF-A, and the accuracy of the combined diagnosis was higher than that of any other single index. The diagnosis scheme involving all three markers was the best (the sensitivity was 60.40%, the specificity was 92.8%, and the accuracy was 53.1%). The best critical values calculated were EphA2 > 297.92 ng/ml, EphA2 > 183.92 pg/ml and CEA > 5.19 ng/ml.

Conclusion: The serum levels of EphA2 and VEGF-A are high in CRC patients, and the combine detection of CEA, EphA2 and VEGF-A can significantly improve the diagnostic accuracy of CRC.
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http://dx.doi.org/10.3233/CBM-201745DOI Listing
January 2021

Macrophage-Derived Exosomal miR-31-5p Promotes Oral Squamous Cell Carcinoma Tumourigenesis Through the Large Tumor Suppressor 2-Mediated Hippo Signalling Pathway.

J Biomed Nanotechnol 2021 May;17(5):822-837

Key Laboratory of Human Functional Genomics of Jiangsu Province, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 211166, Jiangsu, PR China.

Tumour-associated macrophages (TAMs) are thought to contribute to oral squamous cell carcinoma (OSCC) initiation and progression. However, the underlying mechanism through which TAMs foster OSCC progression is still unclear. This study intended to determine whether there are exclusively exosomal miRNAs-derived macrophages that are functionally necessary for OSCC progression. The phenotype of TAM recruitment in OSCC tissue samples was assessed, subsequently identifying the influence of M2 macrophages and exosomes derived from M2 macrophages on OSCC proliferation and tumorigenesis and CD68 and CD163, the specific markers of M2 type macrophages, were upregulated in TAMs presented in intra-cancer tissues. M2 macrophages and M2 macrophage-derived exosomes (M2 exos) both can promote OSCC growth and tumorigenicity. An exosomal RNA-seq analysis was conducted to predict regulatory exosomal miRNAs related to OSCC growth, which determined miR-31-5p and LATS2 for subsequent experiments. Mechanistically, miR-31-5p was delivered to recipient OSCC cells through M2 exos and complementary pairing with the large tumor suppressor 2 (LATS2) coding sequence, thus suppressing the expression of LATS2 and inactivation the Hippo signaling pathway to support OSCC growth. Collectively, our findings demonstrate that M2 macrophage-derived exosomal miR- 31-5p can make tumor suppressor LATS2 gene inhibited and facilitate the progression of OSCC via inhibiting the Hippo signaling pathway, which possibly provides new targets for the molecular therapy of OSCC.
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http://dx.doi.org/10.1166/jbn.2021.3066DOI Listing
May 2021

MiRNA-202-5p promotes Colorectal Carcinogenesis through suppression of PTEN.

J Cancer 2021 31;12(11):3154-3163. Epub 2021 Mar 31.

Department of Immunology, the school of Basic Medical Sciences, Anhui Medical University, Hefei, China, 230032.

Colorectal cancer (CRC) is still one of the leading causes of cancer-associated death in the modern society. The biological function of miR-202-5p for CRC development remains controversial, largely due to the fact that miR-202-5p can be tumor-suppressive or oncogenic in different contexts. Obtained results indicated that aberrant expression of miR-202-5p was observed in majority of human CRC samples and miR-202-5p was transcriptionally up-regulated by c-Myc. In addition, miR-202-5p functions to promote the activation of PI3K/Akt signaling pathway by directly suppressing PTEN. Silencing or enforced expression of miR-202-5p resulted in CRC cell proliferation inhibition and enhancement, respectively. Importantly, decreased PTEN level and increased phosphorylation of Akt were frequently associated with elevated miR-202-5p expression in colorectal cancer tissues. Increased miR-202-5p expression may serve as a tumor promoter by directly targeting PTEN in colorectal cancer.
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http://dx.doi.org/10.7150/jca.56186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100819PMC
March 2021

The Protection of Crocin Against Ulcerative Colitis and Colorectal Cancer via Suppression of NF-κB-Mediated Inflammation.

Front Pharmacol 2021 18;12:639458. Epub 2021 Mar 18.

School of Life Sciences, Jilin University, Changchun, China.

In China, the incidence of ulcerative colitis (UC) is increasing every year, but the etiology of UC remains unclear. UC is known to increase the risk of colorectal cancer (CRC). The aim of this study was to investigate the protective effects of crocin against UC and CRC in mouse models. Crocin was used to treat the dextran sodium sulfate (DSS)-induced UC mice for 3 weeks, and Apc/Gpt mice with colorectal cancer for 8 weeks. Proteomics screening was used to detect changes in the protein profiles of colon tissues of UC mice. Enzyme-linked immunosorbent assays and western blot were used to verify these changes. Crocin strongly reduced the disease activity index scores of UC mice, and improved the pathological symptoms of the colonic epithelium. The anti-inflammatory effects of crocin were indicated by its regulation of the activity of various cytokines, such as interleukins, via the modulation of nuclear factor kappa-B (NF-κB) signaling. Crocin significantly suppressed tumor growth in Apc/Gpt mice and ameliorated pathological alterations in the colon and liver, but had no effects on spleen and kidney. Additionally, crocin significantly decreased the concentrations of interleukins and tumor necrosis factor-α in the sera and colon tissues, suggesting its anti-inflammatory effects related to NF-κB signaling. Finally, 12-h incubation of SW480 cells with crocin caused cell cycle arrest, enhanced the apoptotic rate, promoted the dissipation of mitochondrial membrane potential, and the over-accumulation of reactive oxygen species. From the theoretical analyses, phosphorylated residues on S536 may enhance the protein-protein interactions which may influence the conformational changes in the secondary structure of NF-κB. The protective effects of crocin on UC and CRC were due to its suppression of NF-κB-mediated inflammation.
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http://dx.doi.org/10.3389/fphar.2021.639458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025585PMC
March 2021

Association between the omentin-1 gene rs2274907 A>T polymorphism and colorectal cancer in the Chinese Han population: a case-control study.

J Int Med Res 2021 Apr;49(4):3000605211006522

Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, Hefei, Peoples Republic of China.

Objective: To explore the relationship between the omentin-1 gene rs2274907 A>T polymorphism and colorectal cancer (CRC) in the Chinese Han population.

Methods: rs2274907 A>T was assessed by PCR-restriction fragment length polymorphism analysis. Plasma omentin-1 expression from 358 patients with CRC and 286 healthy controls was analyzed by enzyme-linked immunosorbent assay. CRC and control groups were divided into subgroups according to the body mass index (BMI) threshold of 25 kg/m.

Results: No significant differences were observed between CRC and control groups in terms of genotype or allele frequencies of rs2274907 A>T. Compared with individuals with BMI <25 kg/m and the rs2274907 TT genotype, those with AA+AT genotypes and BMI ≥25 kg/m had a 3.027-fold increased risk of CRC. A significant tendency toward a higher stage of colorectal adenocarcinomas and depth of invasion was observed in individuals with the rs2274907 AA genotype compared with other genotypes.

Conclusions: The omentin-1 gene rs2274907 A>T polymorphism does not seem to play a critical role in the development of CRC in the Chinese Han population, but an interaction between the rs2274907 A allele and BMI may increase the CRC risk. The rs2274907 AA genotype is a potential biomarker for CRC stage progression.
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http://dx.doi.org/10.1177/03000605211006522DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033482PMC
April 2021

The impact of long- and short-term exposure to different ambient air pollutants on cognitive function in China.

Environ Int 2021 06 2;151:106416. Epub 2021 Mar 2.

Beijing Normal University, School of Environment, China. Electronic address:

In the field of environmental health, the impact of air pollution on people's cognitive function is receiving increasing attention. Various air pollution exposures and different exposure periods result in different degrees of damage to cognition. This paper first used CFPS cognitive tests to evaluate the cognitive function of 15,163 adults in 25 provinces of China. Next, based on the geographical location of the population, the kriging interpolation method was applied to evaluate the different exposure periods for various air pollutants (PM, NO and O). Air pollution exposures lasting 3 years and more were referred to in this paper as long-term exposures, while those lasting<3 years were short-term exposures. This paper used an ordinary least squares (OLS) regression model to explore the differential effects of various air pollutant exposures and discussed the impact of long- and short-term exposure to pollutants. Subsequently, Moran's index was used to test the spatial connection for cognitive function, and the spatial error model was used for analysis in the spatial autoregressive model. This research also conducted a heterogeneity study on the justice of air pollutant exposure among people with different characteristics. The population was classified according to cognitive function and geographic location using OLS regression and quantile regression, and a propensity score matching (PSM) model was used for cross-validation to explore whether people with different characteristics and attributes were differentially exposed to air pollution. We found that there were significant negative relationships between air pollutant exposure and cognitive function, especially PM exposure and long-term exposure. In addition, air pollution had significantly different impacts on cognition based on the different characteristics and attributes of the person exposed. This study helps by analyzing the socioeconomic factors that affect the level of exposure and suggests that groups who are vulnerable to environmental pollution should be protected and the occurrence of injustice reduced. The study also provides a reference for the distribution of pollution sources and the allocation of health resources, which can be useful for population distribution planning.
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http://dx.doi.org/10.1016/j.envint.2021.106416DOI Listing
June 2021

ADH2 Is Downregulated by Methylation and Acts as a Novel Biomarker for Breast Carcinoma Prognosis.

Ann Clin Lab Sci 2021 Jan;51(1):12-21

Department of Laboratory Medicine, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou, China

Breast carcinoma (BC) ranks the second leading cause of cancer death in females. Alcohol is consistent risk factor for BC. The alcohol dehydrogenases (ADHs) family is associated with alcohol metabolism in vivo. However, whether ADHs can act as biomarkers for BC and the underlying mechanism of them affecting BC are unclear. In the present study, the expression levels, prognostic values, epigenetic and genetic alterations, and regulatory networks of ADHs were explored in BC using public online database. Among ADHs family, the expression level of ADH2 is remarkably decreased in the BC and high expression level of ADH2 is significantly associated with better overall survival in BC. Decreasing mRNA expression level of ADH2 is due to DNA hypermethylation in the promoter rather than genetic alterations. ADH2 strongly correlates with pathways in glycolysis/gluconeogenesis, fatty acid metabolism, and cytochrome P450 pathways in BC. Our finding provided novel insights into ADH2 in BC and implied that ADH2 could act as a novel biomarker for BC prognosis.
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January 2021

The Association between Purine-Rich Food Intake and Hyperuricemia: A Cross-Sectional Study in Chinese Adult Residents.

Nutrients 2020 Dec 15;12(12). Epub 2020 Dec 15.

Department of Nutrition & Food Hygiene, School of Public Health, Peking University Health Science Center, Haidian District, Beijing 100191, China.

Objective: To explore the correlation between purine-rich food intake and hyperuricemia in Chinese adult residents.

Method: A cross-sectional study was conducted on the purine-rich food intake of Chinese adult residents based on the China Health and Nutrition Survey (CHNS) in 2009. The subjects were divided into hyperuricemia group and nonhyperuricemia group according to serum uric acid level, and the differences of the sociodemographic information (age, gender, and region), health status (weight status, blood pressure, blood sugar status), living habits (alcohol consumption, smoking status) and food intake (purine-rich food, other food) were compared between the two groups. Logistic regressions investigated the associations between the daily intake of purine-rich food (animal-derived food and legumes) and hyperuricemia.

Results: Eventually, 6813 subjects were included in our study, 1111 of them had hyperuricemia. The intake of seafood, legumes, red meat, and poultry all increased the risk of hyperuricemia ( < 0.05), while the intake of purine-rich fungi and purine-rich vegetables did not affect the occurrence of hyperuricemia. Animal-derived food was the main source of purine-rich food consumed by Chinese adult residents (140.67g/day), which had a great impact on hyperuricemia. Finally, after adjusting for gender, age, region, body mass index (BMI), alcohol consumption, hypertension, and refined grains intake, the risk of hyperuricemia increased by 2.40% and 1.10% for each increase of 10 g in animal-derived food intake (OR = 1.024, 95% CI: 1.018-1.030) and legumes intake (OR = 1.011, 95% CI: 1.003-1.019), respectively.

Conclusion: The intake of animal-derived food and legumes were positively correlated with the occurrence of hyperuricemia. Controlling the intake of animal-derived food and legumes would be more beneficial to controlling the risk of hyperuricemia.
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http://dx.doi.org/10.3390/nu12123835DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765492PMC
December 2020

Two cases of autoimmune pancreatitis with diabetes and literature review.

Neuro Endocrinol Lett 2020 Sep;41(3):113-117

Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230032, China.

Objective: To investigate the clinical features of autoimmune pancreatitis (AIP) with diabetes as the first manifestation, improve our understanding of the disease and highlight the recognition of special types of diabetes.

Methods: A retrospective analysis was performed on 2 AIP patients diagnosed with diabetes at the First Affiliated Hospital of Anhui Medical University.

Results: Two elderly patients with new-onset diabetes mellitus were admitted to the hospital with weight loss and yellowing of the skin. Imaging showed pancreatic enlargement, bile duct dilatation, and cholestasis. Auxiliary examination followed by histopathology or experimental hormone therapy revealed elevated IgG4 levels, and the patients were eventually diagnosed with AIP.

Conclusion: For elderly diabetic patients with atypical clinical characteristics, such as unexplained gastrointestinal symptoms or weight loss, IgG4 levels should be examined to rule out diabetes secondary to AIP.
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September 2020

Clinical evaluation of toric intraocular lens implantation based on iTrace wavefront keratometric astigmatism.

BMC Ophthalmol 2020 Nov 16;20(1):450. Epub 2020 Nov 16.

Shanxi Eye Hospital, No. 100 Fudong Street, Taiyuan, Shanxi, 030001, People's Republic of China.

Background: Currently, there is no standard technique for determining corneal astigmatism. The iTrace wavefront aberrometry of cornea calculated steep power and axis based on the best Zernike mathematical fit from all topo data within 4 mm circle. It was supposed to be more accurate than iTrace simulated keratometry which was calculated based on only 4 points on the circle of 3 mm. This aim of this study was to evaluate visual outcomes and rotational stability after toric intraocular lens (IOL) implantation using the wavefront aberrometry of the cornea with iTrace.

Setting: Single site in China, Shanxi Eye Hospital, Shanxi, China.

Design: Prospective case series.

Methods: The study included 85 eyes of 63 patients undergoing phacoemulsification and toric IOL implantation. The IOL power and cylinders were chosen with the help of the iTrace toric planning program using wavefront keratometric astigmatism. Astigmatic changes were assessed using Alpins vector method over a 3-month follow-up period.

Results: Preoperative mean corneal topographic astigmatism was 1.91 diopters (D) ± 0.69 (standard deviation). Postoperative mean refractive astigmatism decreased significantly to 0.48 D ± 0.34. Surgical induced astigmatism was 1.73 D ± 0.77 and the mean correction index was 0.89 ± 0.22, showing a slight undercorrection. The proportion of astigmatism ≤0.50 D increased from 0 to 71.8% postoperatively.

Conclusions: This is the first study on evaluation of clinical outcomes of toric IOL implantation in corneal astigmatism patients using iTrace wavefront keratometric readings. The findings show that use of iTrace built-in toric calculator is safe and effective for planning toric IOL surgery for wavefront keratometric astigmatism.

Trial Registration: Current Controlled Trials ISRCTN94956424 , Retrospectively registered (Date of registration: 05 February 2020).
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http://dx.doi.org/10.1186/s12886-020-01726-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7670723PMC
November 2020

Protective effect of Gloeostereum incarnatum on ulcerative colitis via modulation of Nrf2/NF‑κB signaling in C57BL/6 mice.

Mol Med Rep 2020 Oct 5;22(4):3418-3428. Epub 2020 Aug 5.

Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin 130118, P.R. China.

Chronic non‑specific inflammatory cell infiltration of the colon is generally considered to be the cause of ulcerative colitis (UC). Gloeostereum incarnatum (GI), a fungus rich in amino acids and fatty acids, exhibits a variety of biological functions. In the present study, GI was identified to contain 15 fatty acids, 17 amino acids and 11 metallic elements. The protective effect of GI against UC was investigated in C57BL/6 mice with UC induced by free drinking 3.5% dextran sulfate sodium (DSS). After a 21‑day oral administration, GI prevented weight loss, enhancement of the disease activity index and colonic pathological alterations in mice with UC. GI reduced the levels of pro‑inflammatory factors including interleukin (IL)‑1β, IL‑2, IL‑6 and IL‑12, tumor necrosis factor α and ‑β, interferon α and ‑γ, and pro‑oxidative factors including reactive oxygen species and nitric oxide. In addition, it enhanced the levels of immunological factors including immunoglobulin (Ig)A, IgM and IgG, and antioxidative factors including superoxide dismutase and catalase in the serum and/or colon tissues. GI enhanced the expression levels of nuclear factor erythroid 2‑related factor 2 (Nrf2) and its downstream proteins and suppressed the phosphorylation of NF‑κB signaling in colon tissues. Together, GI was shown to alleviate the physiological and pathological state of DSS‑induced UC in mice via its antioxidant and anti‑inflammatory functions, which may be associated with its modulation of the activation of Nrf2/NF‑κB signaling.
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http://dx.doi.org/10.3892/mmr.2020.11420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7453623PMC
October 2020

Retraction Note to: Attenuation of aortic injury by ursolic acid through RAGE-Nox-NFκB pathway in streptozocin-induced diabetic rats.

Arch Pharm Res 2020 Sep;43(9):983

Pharmacology Department, Henan Provincial Institute of Food and Drug Control, Zhengzhou, 450003, China.

The authors have retracted this article [1] because Fig. 4A and Fig. 4B are same as the left-hand panel and the middle panel respectively in Fig. 1 in an article published in Chinese by another author group [2]. In addition, Associate Professor Xiaoning Li did not give permission to be acknowledged for pathology work. All authors agree with this retraction.
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http://dx.doi.org/10.1007/s12272-020-01262-xDOI Listing
September 2020

Long non-coding RNA MILNR1 retards colorectal cancer growth by inhibiting c-Myc.

Cancer Commun (Lond) 2020 09 22;40(9):456-460. Epub 2020 Jul 22.

Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui, 230032, P. R. China.

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http://dx.doi.org/10.1002/cac2.12079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494064PMC
September 2020

Computed tomography of covid-19 pneumonia.

Authors:
Han Ma Yaqin Zhang

BMJ 2020 07 9;370:m1807. Epub 2020 Jul 9.

Department of Radiology, Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China

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http://dx.doi.org/10.1136/bmj.m1807DOI Listing
July 2020

M2 Macrophage-Derived Exosomes Facilitate HCC Metastasis by Transferring α β Integrin to Tumor Cells.

Hepatology 2021 Apr;73(4):1365-1380

Hepatobiliary Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Background And Aims: The development and progression of hepatocellular carcinoma (HCC) is dependent on its local microenvironment. Tumor-associated macrophages (TAMs) are deemed a key factor for the tumor microenvironment and attribute to contribute to tumor aggressiveness. However, the detailed mechanism underlying the pro-metastatic effect of TAMs on HCC remains undefined.

Approach And Results: The present study proved that TAMs were enriched in HCC. TAMs were characterized by an M2-polarized phenotype and accelerated the migratory potential of HCC cells in vitro and in vivo. Furthermore, we found that M2-derived exosomes induced TAM-mediated pro-migratory activity. With the use of mass spectrometry, we identified that integrin, α β (CD11b/CD18), was notably specific and efficient in M2 macrophage-derived exosomes (M2 exos). Blocking either CD11b and/or CD18 elicited a significant decrease in M2 exos-mediated HCC cell metastasis. Mechanistically, M2 exos mediated an intercellular transfer of the CD11b/CD18, activating the matrix metalloproteinase-9 signaling pathway in recipient HCC cells to support tumor migration.

Conclusions: Collectively, the exosome-mediated transfer of functional CD11b/CD18 protein from TAMs to tumor cells may have the potency to boost the migratory potential of HCC cells, thus providing insights into the mechanism of tumor metastasis.
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http://dx.doi.org/10.1002/hep.31432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360085PMC
April 2021

Radiotherapy Enhancement for Human Pancreatic Carcinoma Using a Peptide-Gold Nanoparticle Hybrid.

J Biomed Nanotechnol 2020 Mar;16(3):352-363

Pancreatic ductal adenocarcinoma (PDAC) is radioresistant. Due to their strong X-ray absorption capacity, gold nanoparticles (AuNPs) have been used as radiosensitizers for cancer therapeutics. Herein, we describe a novel conjugate complex consisting of a peptide for targeting plectin-1 (PTP) specifically expressed on the PDAC cell membrane and AuNPs, termed AuNP-PTP, to be used for PDAC radiotherapy and . Previous studies revealed that compared with unmodified AuNPs, AuNP-PTP along with relevant low-energy X-ray irradiation of 6 MV at a dose of 2 Gy (RF) increased the targeting efficiency and induced apoptosis in treated PANC-1 cells and tumours. Importantly, extensive histopathological examination did not reveal evidence of acute or chronic injury in mice due to AuNPs or AuNP-PTP for up to six weeks despite the presence of X-ray exposure. The delicate AuNP-PTP hybrid provides a novel strategy to enhance radiotherapy efficiency in PDAC treatment.
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http://dx.doi.org/10.1166/jbn.2020.2898DOI Listing
March 2020

Corneal Astigmatism Measurements Comparison among Ray-Tracing Aberrometry, Partial Coherence Interferometry, and Scheimpflug Imaging System.

J Ophthalmol 2020 1;2020:3012748. Epub 2020 Apr 1.

Shanxi Eye Hospital, Taiyuan, Shanxi, China.

Purpose: To investigate interdevice agreement among corneal topography/ray-tracing aberrometry (iTrace), partial coherence interferometry (IOLMaster), and Scheimpflug imaging (Pentacam) for the measurement of corneal astigmatism.

Methods: The analysis included 90 eyes of 90 subjects without ocular disease. The main outcome measures were corneal cylinder power and axis of astigmatism. All corneal astigmatism measurements were converted to two perpendicular components by using vector analysis. Interdevice agreement was assessed using Bland-Altman analysis, paired sample -test, and one-way analysis of variance.

Results: No significant interdevice difference existed in the astigmatism magnitude, cardinal component, and oblique component (all > 0.05). On comparing iTrace wavefront and simulated keratometry (SimK) astigmatism, significant differences were observed in the astigmatism magnitude and oblique component (both < 0.01), but not in the cardinal component (=0.687). On comparing Pentacam pupil 3 mm and corneal vertex 3 mm axial astigmatism, significant difference was observed in the astigmatism magnitude ( < 0.001), but not in the cardinal and oblique components (both > 0.05).

Conclusions: The iTrace, IOLMaster, and Pentacam devices could be used interchangeably for corneal astigmatism measurement. However, the measurement difference in iTrace wavefront and SimK astigmatism and Pentacam pupil 3 mm and vertex 3 mm axial astigmatism should be considered in clinic practice.
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http://dx.doi.org/10.1155/2020/3012748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7152950PMC
April 2020

Automated fibroglandular tissue segmentation in breast MRI using generative adversarial networks.

Phys Med Biol 2020 05 19;65(10):105006. Epub 2020 May 19.

School of Data and Computer Science, Sun Yat-Sen University, Guangzhou, People's Republic of China. Guangdong Province Key Laboratory Computational Science, Sun Yat-Sen University, Guangzhou, People's Republic of China.

Fibroglandular tissue (FGT) segmentation is a crucial step for quantitative analysis of background parenchymal enhancement (BPE) in magnetic resonance imaging (MRI), which is useful for breast cancer risk assessment. In this study, we develop an automated deep learning method based on a generative adversarial network (GAN) to identify the FGT region in MRI volumes and evaluate its impact on a specific clinical application. The GAN consists of an improved U-Net as a generator to generate FGT candidate areas and a patch deep convolutional neural network (DCNN) as a discriminator to evaluate the authenticity of the synthetic FGT region. The proposed method has two improvements compared to the classical U-Net: (1) the improved U-Net is designed to extract more features of the FGT region for a more accurate description of the FGT region; (2) a patch DCNN is designed for discriminating the authenticity of the FGT region generated by the improved U-Net, which makes the segmentation result more stable and accurate. A dataset of 100 three-dimensional (3D) bilateral breast MRI scans from 100 patients (aged 22-78 years) was used in this study with Institutional Review Board (IRB) approval. 3D hand-segmented FGT areas for all breasts were provided as a reference standard. Five-fold cross-validation was used in training and testing of the models. The Dice similarity coefficient (DSC) and Jaccard index (JI) values were evaluated to measure the segmentation accuracy. The previous method using classical U-Net was used as a baseline in this study. In the five partitions of the cross-validation set, the GAN achieved DSC and JI values of 87.0 ± 7.0% and 77.6 ± 10.1%, respectively, while the corresponding values obtained through by the baseline method were 81.1 ± 8.7% and 69.0 ± 11.3%, respectively. The proposed method is significantly superior to the previous method using U-Net. The FGT segmentation impacted the BPE quantification application in the following manner: the correlation coefficients between the quantified BPE value and BI-RADS BPE categories provided by the radiologist were 0.46 ± 0.15 (best: 0.63) based on GAN segmented FGT areas, while the corresponding correlation coefficients were 0.41 ± 0.16 (best: 0.60) based on baseline U-Net segmented FGT areas. BPE can be quantified better using the FGT areas segmented by the proposed GAN model than using the FGT areas segmented by the baseline U-Net.
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http://dx.doi.org/10.1088/1361-6560/ab7e7fDOI Listing
May 2020

HRD1, an Important Player in Pancreatic β-Cell Failure and Therapeutic Target for Type 2 Diabetic Mice.

Diabetes 2020 05 21;69(5):940-953. Epub 2020 Feb 21.

Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing, Jiangsu, China

Inadequate insulin secretion in response to glucose is an important factor for β-cell failure in type 2 diabetes (T2D). Although HMG-CoA reductase degradation 1 (HRD1), a subunit of the endoplasmic reticulum-associated degradation complex, plays a pivotal role in β-cell function, HRD1 elevation in a diabetic setting contributes to β-cell dysfunction. We report in this study the excessive HRD1 expression in islets from humans with T2D and T2D mice. Functional studies reveal that β-cell-specific HRD1 overexpression triggers impaired insulin secretion that will ultimately lead to severe hyperglycemia; by contrast, HRD1 knockdown improves glucose control and response in diabetic models. Proteomic analysis results reveal a large HRD1 interactome, which includes v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (MafA), a master regulator of genes implicated in the maintenance of β-cell function. Furthermore, mechanistic assay results indicate that HRD1 is a novel E3 ubiquitin ligase that targets MafA for ubiquitination and degradation in diabetic β-cells, resulting in cytoplasmic accumulation of MafA and in the reduction of its biological function in the nucleus. Our results not only reveal the pathological importance of excessive HRD1 in β-cell dysfunction but also establish the therapeutic importance of targeting HRD1 in order to prevent MafA loss and suppress the development of T2D.
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http://dx.doi.org/10.2337/db19-1060DOI Listing
May 2020

Cytotoxicity of amide-linked local anesthetics on melanoma cells via inhibition of Ras and RhoA signaling independent of sodium channel blockade.

BMC Anesthesiol 2020 02 21;20(1):43. Epub 2020 Feb 21.

Department of Anesthesia, Wuhan Fourth Hospital; Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, 473 Hanzheng Street, Qiaokou District, Wuhan, 430033, Hubei, China.

Background: Substantial clinical and preclinical evidence have indicated the association between amide-linked local anesthesia and the long-term outcomes of cancer patients. However, the potential effects of local anesthesia on cancer recurrence are inconclusive and the underlying mechanisms remain poorly understood.

Methods: We systematically examined the effects of three commonly used local anesthetics in melanoma cells and analyzed the underlying mechanisms focusing on small GTPases.

Results: Ropivacaine and lidocaine but not bupivacaine inhibited migration and proliferation, and induced apoptosis in melanoma cells. In addition, ropivacaine and lidocaine but not bupivacaine significantly augmented the in vitro efficacy of vemurafenib (a B-Raf inhibitor for melanoma with BRAF V600E mutation) and dacarbazine (a chemotherapeutic drug). Mechanistically, ropivacaine but not bupivacaine decreased the activities of Ras superfamily members with the dominant inhibitory effects on RhoA and Ras, independent of sodium channel blockade. Rescue studies using constitutively active Ras and Rho activator calpeptin demonstrated that ropivacaine inhibited migration mainly through RhoA whereas growth and survival were mainly inhibited through Ras in melanoma cells. We further detected a global reduction of downstream signaling of Ras and RhoA in ropivacaine-treated melanoma cells.

Conclusion: Our study is the first to demonstrate the anti-melanoma activity of ropivacaine and lidocaine but not bupivacaine, via targeting small GTPases. Our findings provide preclinical evidence on how amide-linked local anesthetics could affect melanoma patients.
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http://dx.doi.org/10.1186/s12871-020-00957-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033929PMC
February 2020

The association between Chinese patients' elevated omentin-1 levels, their clinicopathological features, and the risk of colorectal cancer.

Int J Clin Exp Pathol 2019 1;12(6):2264-2274. Epub 2019 Jun 1.

Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University Hefei, Anhui, People's Republic of China.

To investigate the association between plasma omentin-1 levels and the risk of colorectal cancer (CRC) and the pathological changes of CRC, a total of 358 colorectal adenocarcinoma patients were included in the experience group, and 286 people were included in the control group. Their levels of omentin-1, adiponectin, visfatin, leptin, and their anthropometric and metabolic parameters were determined, and we analyzed the tertile distributions in the control group, according to the different levels: low, medium, and high. The results showed that the omentin-1 levels in patients with CRC were higher than the levels in the controls [(67.28 ± 32.25) vs (33.16 ± 19.93) ng/mL, P = 0.005]. The patients with the highest concentration of omentin-1 presented significantly higher odds for CRC, adjusted for potential confounding factors for CRC (odds ratio: 5.76; 95% CI 1.81-8.95; P = 0.001). The plasma omentin-1 level in CRC yielded a receiver operating characteristic curve area of 88.4%. The optimal sensitivity and specificity were 81.2% and 69.8% in discriminating CRC from the normal control. A high omentin-1 level was significantly associated the increasing stage of colorectal adenocarcinomas and the depth of invasion (P = 0.005, 0.026, respectively). The present study suggests an increased level of omentin-1 not only was a strong risk factor for CRC but could also represent a potential biomarker for CRC stage progression and CRC diagnosis in Chinese patients.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949648PMC
June 2019

Autocrine action of adipokine omentin-1 in the SW480 colon cancer cell line.

Oncol Lett 2020 Jan 21;19(1):892-898. Epub 2019 Nov 21.

Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China.

Omentin-1, a 34-kDa protein, has been demonstrated to be associated with colorectal cancer (CRC). Epidemiological and clinical studies have indicated that the levels of circulating omentin-1 are significantly increased in patients with CRC, but the cause of the high omentin-1 levels and whether CRC cells express this adipokine have not been determined. In the present study, human colorectal carcinoma and para-carcinoma tissues were collected from 24 patients with CRC. In addition, SW480 and HCT116 colon cancer cells were cultured . The expression and localization of omentin-1 protein in human CRC and para-carcinoma tissues were determined by immunohistochemistry. The mRNA and protein expression levels of omentin-1 in human CRC and para-carcinoma tissues were detected by reverse transcription-quantitative PCR (RT-qPCR) and western blotting, respectively. In addition, omentin-1 mRNA expression levels in SW480 and HCT116 cell lines were detected by RT-qPCR. Since SW480 cells exhibited higher omentin-1 mRNA levels compared with those of HCT116 cells, SW480 cells were selected for further experiments. The expression of omentin-1 protein in the supernatant and lysate of SW480 cells obtained at 6, 12, 24 and 48 h was determined by ELISA. The immunohistochemistry results demonstrated that the positive expression of omentin-1 protein was mainly located in the cytoplasm of cancer cells in human CRC tissues. The mRNA and protein expression levels of omentin-1 in the CRC tissues were higher compared with those in para-carcinoma tissues. The expression levels of omentin-1 were detected in the cell lysate and supernatant of SW480 cells; the expression level of omentin-1 protein in the cell lysate was higher compared with that in the supernatant. These results indicated that SW480 cells secret and express the adipokine omentin-1 endogenously. Omentin-1 may serve its potential carcinogenetic role in CRC through endocrine, autocrine and paracrine pathways.
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http://dx.doi.org/10.3892/ol.2019.11131DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6924134PMC
January 2020

Automatic segmentation of the pectoral muscle based on boundary identification and shape prediction.

Phys Med Biol 2020 02 19;65(4):045016. Epub 2020 Feb 19.

School of Data and Computer Science, Sun Yat-sen University, No. 135 Xin Gang Road West, Guangzhou, People's Republic of China.

The purpose of this work is to identify the pectoral muscle region in mediolateral oblique (MLO) view mammograms even when the boundary is blurred or obscured. The problem is decoupled into two subproblems in our study: identifying parts of boundaries with high confidence and predicting the overall shape of the pectoral muscle. Due to the similarity in intensity and texture between pectoral muscle and gland tissue, we trained a deep neural network to distinguish them in the first subproblem. The boundary with high confidence can be obtained according to the consistency of predictions from multiple converged models. For the shape prediction problem, a generative adversarial network (GAN) is used to learn mapping from a given identified region and the breast shape to the overall pectoral muscle shape. Our method is evaluated on a mammogram dataset including 633 MLO view mammograms collected from three different datacenters. We take U-Net as our baseline model and the dataset is divided into three groups according to the performance of U-Net for evaluation. In all three groups, U-Net achieves 80.1%, 92.9%, and 98.3% in the Dice similarity coefficient, respectively, and our method achieves 85.2%, 94.8%, and 98.1% in the Dice similarity coefficient, respectively. The experiment shows that our method effectively estimates the pectoral muscle boundary, even parts of boundaries that are difficult to detect, and greatly improves the performance of segmentation in this case.
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http://dx.doi.org/10.1088/1361-6560/ab652bDOI Listing
February 2020
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