Publications by authors named "Yaqin Hou"

20 Publications

  • Page 1 of 1

[F]FDG PET/MRI and magnetoencephalography may improve presurgical localization of temporal lobe epilepsy.

Eur Radiol 2021 Oct 14. Epub 2021 Oct 14.

Department of Radiology and Nuclear Medicine, Xuanwu Hospital Capital Medical University, Beijing, 100053, China.

Objectives: To evaluate the clinical value of the combination of [F]FDG PET/MRI and magnetoencephalography (MEG) ([F]FDG PET/MRI/MEG) in localizing the epileptogenic zone (EZ) in temporal lobe epilepsy (TLE) patients.

Methods: Seventy-three patients with localization-related TLE who underwent [F]FDG PET/MRI and MEG were enrolled retrospectively. PET/MRI images were interpreted by two radiologists; the focal hypometabolism on PET was identified using statistical parametric mapping (SPM). MEG spike sources were co-registered onto T1-weighted sequence and analyzed by Neuromag software. The clinical value of [F]FDG PET/MRI, MEG, and PET/MRI/MEG in locating the EZ was assessed using cortical resection and surgical outcomes as criteria. The correlations between surgical outcomes and modalities concordant or non-concordant with cortical resection were analyzed.

Results: For 46.6% (34/73) of patients, MRI showed definitely structural abnormality concordant with surgical resection. SPM results of [F]FDG PET showed focal temporal lobe hypometabolism concordant with surgical resection in 67.1% (49/73) of patients, while the concordant cases increased to 82.2% (60/73) patients with simultaneous MRI co-registration. MEG was concordant with surgical resection in 71.2% (52/73) of patients. The lobar localization was defined in 94.5% (69/73) of patients by the [F]FDG PET/MRI/MEG. The results of PET/MRI/MEG concordance with surgical resection were significantly higher than that of PET/MRI or MEG (χ = 13.948, p < 0.001; χ = 5.393, p = 0.020). The results of PET/MRI/MEG cortical resection concordance with surgical outcome were shown to be better than PET/MRI or MEG (χ = 6.695, p = 0.012; χ = 16.991, p < 0.0001).

Conclusions: Presurgical evaluation by [F]FDG PET/MRI/MEG could improve the identification of the EZ in TLE and may further guide surgical decision-making.

Key Points: • Lobar localization was defined in 94.5% of patients by the [F]FDG PET/MRI/MEG. • The results of PET/MRI/MEG concordance with surgical resection were significantly higher than that of PET/MRI or MEG alone. • The results of PET/MRI/MEG cortical resection concordance with surgical outcome were shown to be better than that of PET/MRI or MEG alone.
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http://dx.doi.org/10.1007/s00330-021-08336-4DOI Listing
October 2021

Insight into the enhancing activity and stability of Ce modified VO/AC during cyclic desulfurization-regeneration-denitrification.

J Hazard Mater 2021 Sep 30;424(Pt B):127397. Epub 2021 Sep 30.

State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan 030001, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Cyclic desulfurization-regeneration-denitrification over carbon-based catalysts is a promising technology for SO and NOx simultaneous elimination in steel industry. Regeneration is imperative to the long-term operation of the process, while the research is limited. In this work, Ce modified VO/AC catalyst (CeVOx/AC) with higher desulfurization and denitrification activity was prepared and the effect of cyclic regeneration was investigated. Results illustrated that the desulfurization and denitrification activity of CeVOx/AC gradually improved with increasing the regeneration cycles at the optimum regeneration temperature of 470 °C in N. The increasing Ce, V and oxygen vacancies, enhanced surface acidity and improved redox ability contributed to the catalytic activity of regenerated catalysts. For desulfurization, more SO transformed into HSO rather than to metal sulfates after cyclic regeneration. For denitrification, the improved redox ability accelerated the oxidation of NO to active NO, bridged nitrites and nitrates, and the enhanced acidity facilitated the NH adsorption, further generating more -NH and promoting the SCR activity of regenerated samples. The CeVOx/AC with good activity and regenerative stability shows great application potential in steel industry for the simultaneous SO and NOx removal.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127397DOI Listing
September 2021

[Prenatal cytogenetic and molecular genetic analysis of a fetus with confined placenta mosaicism for trisomy 16].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Aug;38(8):771-774

Genetics and Prenatal Diagnosis Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.

Objective: To review the clinical data of a fetus with false positive result of non-invasive prenatal testing (NIPT) due to confined placental mosaicism (CPM).

Methods: Amniotic fluid sample was taken from a pregnant women with high risk for chromosome 16 aneuploidy for karyotyping analysis, single nucleotide polymorphism array (SNP array) and interphase fluorescence in situ hybridization (FISH). Genetic testing was also conducted on the fetal and maternal surface of the placenta, root of umbilical cord and fetal skin tissue after induced abortion.

Results: Cytogenetic analysis of the amniotic fluid sample yielded a normal karyotype. SNP array revealed mosaicism (20%) of trisomy 16 in the fetus. FISH confirmed the presence of mosaicism (25%) for trisomy 16. After induced labor, all sampled sites of placenta were confirmed to contain trisomy 16 by SNP array, while the analysis of fetal skin tissue yielded a negative result.

Conclusion: CPM is an important factor for false positive NIPT result. Prenatal identification of CPM and strengthened pregnancy management are important to reduce adverse pregnancy outcomes.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200609-00425DOI Listing
August 2021

[Prenatal diagnosis and family analysis of 22q11.2 microdeletion syndrome].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Jul;38(7):659-662

Genetic and Prenatal Diagnosis Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, China.

Objective: To analyze the prenatal diagnosis, parental verification and pregnancy outcome of 6 fetuses with 22q11.2 microdeletion syndrome.

Methods: Copy number variation sequencing (CNV-seq)and chromosomal microarray analysis (CMA) were carried out for the fetuses.

Results: The fetuses were found to harbor 2.54-3.2 Mb microdeletions of the 22q11.2 region, among which one was maternally inherited and one was paternally inherited. Two parents opted to continue with the pregnancy, and 4 chose induced labor. One fetus was found to have tetralogy of Fallot, while two carrier parents and one fetus appeared to have normal phenotype.

Conclusion: 22q11.2 microdeletions identified upon prenatal diagnosis should be treated carefully, with ultrasonic scan and parental verification taken into account.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200402-00234DOI Listing
July 2021

The potential of expanded noninvasive prenatal screening for detection of microdeletion and microduplication syndromes.

Prenat Diagn 2021 Sep 3;41(10):1332-1342. Epub 2021 Jul 3.

Genetic and Prenatal Diagnosis Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Objectives: To evaluate the clinical potential of a higher resolution noninvasive prenatal screening (NIPS-Plus) test for detection of microdeletion/microduplication syndromes (MMS) in addition to common aneuploidies.

Methods: In a multicenter prospective study, 37,002 pregnant women with unremarkable first-trimester ultrasound scans had a NIPS-Plus test. Ultrasound screen positive women were not included in this study.

Results: Of 36,970 ultrasound negative women there were 291 NIPS-Plus screen positive results indicating 237 aneuploidies and 54 MMS. Following amniocentesis, 171 (72%) were confirmed as genuine, comprising 3 T13s, 10 T18s, 61 T21s, 70 SCAs and 27 MMS. The PPV for MMS with unremarkable ultrasound findings was 50%. Routine clinical examination of children born from NIPS-Plus negative pregnancies revealed no obvious signs of chromosome disease syndromes at one year of age.

Conclusions: NIPS-Plus has the potential for clinical utility not only for routine aneuploid screening but also for MMS that do not show overt signs during early pregnancy ultrasound screening. We suggest that ultrasound with NIPS-Plus in combination with appropriate counselling could be considered as a comprehensive first-tier prenatal screening approach for all pregnant women.
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http://dx.doi.org/10.1002/pd.6002DOI Listing
September 2021

Crossed cerebellar diaschisis: risk factors and prognostic value in focal cortical dysplasia by F-FDG PET/CT.

Ann Nucl Med 2021 Jun 11;35(6):719-727. Epub 2021 Apr 11.

Department of Nuclear Medicine, Xuanwu Hospital, Capital Medical University, No. 45 Changchun Street, Xicheng District, Beijing, 100053, China.

Purpose: Crossed cerebellar diaschisis (CCD) has been widely studied in hemispheric stroke but is less characterized in epilepsy. In this study, we used F-FDG PET/CT to investigate the risk factors for CCD and its prognostic value for intractable epilepsy caused by focal cortical dysplasia (FCD).

Methods: One hundred medically intractable epilepsy patients pathologically diagnosed with FCD postoperatively were included and classified into two groups: CCD+ and CCD-. PET/CT images were analyzed qualitatively by visual assessment and semi-quantitatively using the absolute asymmetry index (|AI|). Clinical factors, including age, sex, body mass index (BMI), age at seizure onset, epilepsy duration, seizure type, seizure frequency, electroencephalography (EEG) and brain magnetic resonance imaging (MRI), were retrospectively assessed from medical records. Follow-up outcomes were evaluated according to the Engel classification at 3, 6, 12, 24 and 36 months postoperatively.

Results: Of the 100 patients, 77 (77.0%) were classified as CCD-, and 23 (23.0%) were classified as CCD + . CCD+ patients had a higher number of lobes involved on PET (3.61 ± 2.16 vs 2.26 ± 1.01, P < 0.001) than CCD- patients. CCD- patients showed more negative MRI results than CCD + patients (P = 0.02). At 12 months postoperatively, 70.7% (29/41) of CCD- patients and 30.8% (4/13) of CCD + patients presented a favourable prognosis (P = 0.02). Significant differences in the average |AI| values in the posterior frontal and anterior temporal lobes were found between CCD+ and CCD- patients (P < 0.05).

Conclusion: The number of lobes involved on PET, structural anomalies on MRI, the |AI| values in the posterior frontal and anterior temporal lobes may be predisposing factors for CCD. CCD occurrence may help predict the prognosis of FCD patients at 12 months postoperatively.
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http://dx.doi.org/10.1007/s12149-021-01613-8DOI Listing
June 2021

[Analysis of genetic variants in five pedigrees affected with Dysferlinopathy].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Mar;38(3):205-209

Prenatal Diagnosis Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.

Objective: To analyze the clinical phenotype and genetic variants in five Chinese pedigrees affected with Dysferlinopathy.

Methods: Next generation sequencing (NGS) was carried out for the probands from the five pedigrees. Suspected variants were validated by Sanger sequencing. Pathogenicity of the variants was assessed based on the standards and guidelines by the American College of Medical Genetics and Genomics (ACMG).

Results: Ten DYSF gene variants (including 5 frameshift variants, 3 splicing variants, 1 missense variant and 1 nonsense variant) were detected. Among these, c.1375dupA (p.Met459Asnfs*15), c.610C>T (p.Arg204X), c.1180+5G>A and c.1284+2T>C were known to be pathogenic, while c.4008_4010delCCTinsAC (p.Leu1337Argfs*8), c.1137_1169del (p.379_390del), c.754A>G(p.Thr252Ala), c.1175_1176insGCAGAGTG (p.Met394Serfs*7), c.3114_3115insCGGC (p.Arg1040Profs*74) and c.1053+3G>C were unreported previously. Of the six novel variants, c.1137_1169del, c.1175_1176insGCAGAGTG and c.3114_3115insCGGC were predicted as pathogenic (PVS1+PM2+PM3), c.4008_4010delCCTinsAC as likely pathogenic (PVS1+PM2), c.754A>G and c.1053+3G>C as variants of uncertain significance based on the ACMG standards and guidelines.

Conclusion: Variants of the DYSF gene probably underlay Dysferlinopathy in the patients among the five pedigrees. Above finding has enriched the spectrum of DYSF gene variants.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200228-00112DOI Listing
March 2021

Assessment of localization accuracy and postsurgical prediction of simultaneous F-FDG PET/MRI in refractory epilepsy patients.

Eur Radiol 2021 Sep 27;31(9):6974-6982. Epub 2021 Feb 27.

Department of Nuclear Medicine, Xuanwu Hospital, Capital Medical University, Beijing, China.

Objectives: To evaluate the accuracies of simultaneous F-fluorodeoxyglucose positron emission tomography/magnetic resonance imaging ([F]-FDG PET/MRI) in preoperative localization and the postsurgical prediction.

Methods: This retrospective study was performed on ninety-eight patients diagnosed with refractory epilepsy whose presurgical evaluation included [F]-FDG PET/MRI, with 1-year post-surgery follow-up between August 2016 and December 2018. PET/MRI images were interpreted by two radiologists and a nuclear medicine physician to localize the EOZ using standard visual analysis and asymmetry index based on standard uptake value (SUV). The localization accuracy and predictive performance of simultaneous F-FDG PET/MRI based on the surgial pathology and postsurgical outcome were evaluated.

Results: A total of 41.8% (41/98) patients were found to have a definitely structural abnormality on the MR portion of PET/MRI; 93.9% (92/98) were shown hypometabolism on the PET portion of the hybrid PET/MRI. PET/MRI identified 18 cases with subtle structural abnormalities on MRI re-read. Six percent (6/98) of patients PET/MRI were negative. A total of 65.3% (64/98) patients showed seizure-free at 1-year follow-up after epilepsy surgery. The sensitivity, specificity, and accuracy of [F]-FDG PET/MRI was 95.3%, 8.8%, and 65.3% for seizure onset localization based on surgical pathology and postsurgical outcome, respectively. Multivariate regression analysis indicated that concordant of EOZ localization between PET/MRI and surgical resection range, which was a good positive predictor of seizure freedom (Engel I) (OR = 14.741, 95% CI 3.934-55.033, p < 0.001).

Conclusions: [F]-FDG PET/MRI used as two combined modalities providing additional sensitivity when detecting possible epileptic foci and will probably improve the surgical outcome.

Key Points: • Sensitivity, specificity, and accuracy of [F]-FDG PET/MRI were 95.3%, 8.8%, and 65.3% for seizure onset localization based on surgical pathology and postsurgical outcome, respectively. • Concordance of EOZ localization between PET/MRI and surgical resection range was a good positive predictor of seizure freedom; presurgical [F]-FDG PET/MRI will probably improve the surgical outcome.
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http://dx.doi.org/10.1007/s00330-021-07738-8DOI Listing
September 2021

[Detection of genomic copy number variations in patients with unexplained mental retardation/developmental delay by low coverage whole-genome sequencing].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2020 Sep;37(9):953-957

Prenatal Diagnosis Center, General Hospital of Wanbei Coal and Power Group, Suzhou, Anhui 234000, China.

Objective: To detect genomic copy number variations (CNVs) among 145 children with unexplained mental retardation/developmental delay (MR/DD) by using low-depth whole-genome copy number variation sequencing (CNV-seq).

Methods: Peripheral blood samples were collected from the patients and subjected to DNA extraction and CNV-seq. The results were analyzed by a combination of bioinformatic tools.

Results: Forty-nine patients were found to carry a total of 67 CNVs with an average size of 5.27 Mb. Among these, 22 patients were assessed to carry MR/DD-related CNVs involving 21 syndromes. This gave a diagnostic rate of 15.17%(22/145) for CNVs associated with unexplained MR/DD. The corresponding regions of the 22 MR/DD-related CNVs in the human genome covered 174 MR/DD-related pathogenic genes, which have mapped to 18 sections on 10 chromosomes.

Conclusion: Genomic CNVs-related microdeletions/duplications account for a significant proportion of unexplained MR/DD, for which CNV-seq can provide an accurate diagnosis.
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http://dx.doi.org/10.3760/cma.j.cn511374-20190922-00488DOI Listing
September 2020

[Phenotypic and genetic characteristics of a child with 7p15 deletion syndrome].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2020 Aug;37(8):855-858

Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.

Objective: To explore the genetic basis for a child with multiple malformation and growth retardation.

Methods: The child was subjected to low-coverage massively parallel copy number variation sequencing (CNV-seq) based on next generation sequencing (NGS) technique.

Results: G-banding karyotyping analysis has found no abnormality in the boy and his parents. CNV-seq analysis discovered that the child has carried a heterozygous 4.36 Mb deletion (24 020 000-28 380 000) at 7p15.3p15.1. The same deletion was not found in either parent. The deletion has encompassed 28 OMIM genes including HOXA13, CYCS, DFNA5, HOXA11 and HOXA2. Among these, HOXA13 has been associated with distal limb deformity, hypospadias and cryptorchidism. HOXA1, HOXA3 and HOXA4 are involved in the formation of cardiac primordia and primordial tube, and HOXA2 is involved in the development of auditory system. The clinical phenotype of the child was consistent with that of 7p15 deletion syndrome.

Conclusion: Haploinsufficiency of HOXA1, HOXA2, HOXA3, HOXA4 and HOXA13 genes may underlie the clinical phenotype of the child, which is comparable to 7p15 deletion syndrome.
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http://dx.doi.org/10.3760/cma.j.issn.1003-9406.2020.08.012DOI Listing
August 2020

Arterial spin labeling-MR may be an alternative to SPECT for evaluating cerebral perfusion in patients with unilateral middle cerebral artery stenosis.

Neurol Res 2020 Aug 13;42(8):621-629. Epub 2020 Jul 13.

Department of Neurology, Xuanwu Hospital, Capital Medical University , Beijing, China.

Objective: Cerebral blood flow (CBF) mapping of single-photon emission tomography (SPECT) is considered a gold standard for evaluating cerebral perfusion. However, invasiveness, high costs and strict technical requirements can limit its clinical use. We aimed to evaluate the concordance of CBF maps obtained from SPECT and pseudo-continuous arterial spin labeling magnetic resonance (PCASL-MR) imaging for evaluating cerebral perfusion.

Methods: PCASL-MR/SPECT-CBF maps were obtained from 16 eligible patients with unilateral middle cerebral artery stenosis (MCAS). Three slices (basal ganglia, semi-oval center and cerebellum) on both PCASL-MR and SPECT maps were divided into different regions of interest (ROIs) according to the ASPECT criterion, arterial territories, and cerebral hemispheres, respectively. The concordance of the two types of CBF maps and the specificity and sensitivity of PCASL-MR imaging on predicting regional hypoperfusion were calculated.

Results: A total of 448 ROIs were divided according to the ASPECT criterion, 192 ROIs partitioned in accordance with arterial territories, and 96 ROIs delineated based on cerebral hemispheres were analyzed. PCASL-MR imaging exhibited 83.78% to 100% sensitivity, 90.19% to 95.83% specificity for detection of hypoperfusion. Qualitative analyses revealed a strong concordance between PCASL-MR and SPECT on reflecting regional cerebral hypoperfusion (Kappa coefficient = 0.662-0.920, p < 0.01). Semi-quantitative analysis by ΔCBF revealed moderate consistency (Spearman correlation coefficient = 0.610-0.571).

Conclusions: Our findings suggest that PCASL-MR may be a promising non-invasive, inexpensive alternative to SPECT for evaluating cerebral perfusion accurately in patients with symptomatic MCAS.
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http://dx.doi.org/10.1080/01616412.2020.1782080DOI Listing
August 2020

A new insight into the promotional effect of nitrogen-doping in activated carbon for selective catalytic reduction of NO with NH.

Sci Total Environ 2020 Oct 12;740:140158. Epub 2020 Jun 12.

State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan 030001, PR China. Electronic address:

A series of N-doped carbons were prepared to investigate the effect of different N-containing groups on selective catalytic reduction (SCR) of NOx with NH. Combined the SCR activity with the results of porosity analysis and X-ray photoelectron spectroscopy, it's deduced that the pyridinic N (N-6) rather than the surface area or doped total N was mainly responsible for the promoted SCR activity. The electron paramagnetic resonance and O-temperature programmed desorption (O-TPD) experiments indicated that N-6 created numerous of oxygen vacancy. The NO+O-TPD and transient response of NH further demonstrated that the increased oxygen vacancy enhanced the absorbability and reactivity of NOx, therefore the SCR reaction was elevated by accelerating the reaction in the Langmuir-Hinshelwood (L-H) mechanism. Furthermore, the NH-TPD suggested that N-6 was conductive to the NH adsorption. In situ DRIFTs of NH adsorption and reaction illustrated that the increased NH mainly existed as NH species, which were quickly consumed by NO+O, further elevated the reaction between gaseous NO and adsorbed NH in the Eley-Rideal (E-R) mechanism. The N-6 groups doped in the activated carbons facilitated the L-H and E-R reactions and thus promoted the SCR activity.
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http://dx.doi.org/10.1016/j.scitotenv.2020.140158DOI Listing
October 2020

Effect of triglyceride on complexation between starch and fatty acid.

Int J Biol Macromol 2020 Jul 8;155:1069-1074. Epub 2019 Nov 8.

The State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, China. Electronic address:

This study aimed to reveal the effect of corn oil on formation of complex between rice starch and fatty acids, including lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0) and stearic acid (C18:0). The RVA, XRD, DSC and CLSM analysis confirmed formation of starch-fatty acid complex but the starch-triglyceride complex was not detected. The addition of triglyceride promoted formation of starch-C16:0 and starch-C18:0 complexes, which might be due to more leached amylose induced by oil. However, triglyceride inhibited formation of starch-C12:0 and starch-C14:0 complexes, which might be due to high solubility of C12:0 and C14:0 in oil. In conclusion, the effect of triglyceride on formation of starch-fatty acid complex depended on the chain length of fatty acids.
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http://dx.doi.org/10.1016/j.ijbiomac.2019.11.072DOI Listing
July 2020

Dopaminergic pathway and primary visual cortex are involved in the freezing of gait in Parkinson's disease: a PET-CT study.

Neuropsychiatr Dis Treat 2019 10;15:1905-1914. Epub 2019 Jul 10.

The Department of Neurology, Xuanwu Hospital of Capital Medical University, Beijing, People's Republic of China.

Freezing of gait (FOG) could be partly alleviated by dopaminergic drugs but the mechanism still needs to be elucidated. The purpose of this study is to explore the mechanisms of FOG by vesicular monoamine transporter VMAT2 distribution with the F-AV133 tracer and 18-fludeoxyglucose positron emission tomography-computed tomography (F-FDG PET-CT). Clinical material and PET-CT data were collected from 20 patients with FOG and 147 patients without FOG from November 1, 2017 to October 31, 2018. Brain scans of all participants were acquired over an approximately 20-min period, 120 min after injection of approximately 250 MBq F-AV133. The mean uptake ratios of different regions were identified by NeuroQ software of F-FDG PET-CT. Data analysis included variance, chi-square analysis, covariance analysis, and logistic regression. Our data showed that patients with FOG were provided with greater doses of dopaminergic drugs (<0.05). The frequency of FOG was 11.98% and increased as Parkinson's disease progressed. FOG was more common in the elderly and strongly associated with the duration. Cognitive impairments were obvious, assessed by Mini-Mental State Examination and Montreal Cognitive Assessment (<0.05). The VMAT2 distribution with F-AV133 was decreased significantly in the caudate nucleus and lentiform nucleus while the metabolism of these areas was elevated, determined by F-FDG PET-CT (<0.05). The metabolism of the primary visual cortex decreased obviously in patients with FOG compared with those without FOG (<0.05). FOG mainly occurred in the advanced stage, and was strongly associated with the duration and larger dose of dopaminergic drugs. The dopamine level of the nigrostriatal system decreased significantly and the uptake ratios of the primary visual cortex dropped obviously in the FOG group compared with the non-FOG group. Our study suggests that both the dopaminergic pathway and the primary visual cortex are involved in the pathogenesis of FOG.
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http://dx.doi.org/10.2147/NDT.S197879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6645693PMC
July 2019

Effect of SCR Atmosphere on the Removal of Hg by a VO-CeO/AC Catalyst at Low Temperature.

Environ Sci Technol 2019 05 15;53(9):5521-5527. Epub 2019 Apr 15.

State Key Laboratory of Coal Conversion , Institute of Coal Chemistry Chinese Academy of Sciences , Taiyuan , Shanxi 030001 , P. R. China.

A series of VO-xCeO/AC (noted as V-Ce/AC) catalysts were synthesized by the impregnation method, which combined the advantage of AC and V-Ce. The effects of SCR atmosphere on Hg removal were systematically investigated at low temperature. The experimental results indicated that NO had a positive effect on Hg removal. In addition, an interesting experimental phenomenon was found that NH also showed a positive effect on Hg removal, which is different from many studies that have reported a negative effect of NH on Hg removal by other catalysts. NH-TPD experiment showed that there was no apparent competition between NH and Hg. An FT-IR gas analyzer and in situ DRIFTS were used to study the mechanism for the effect of NH on the catalyst surface and found that a small part of NH was overoxidized to NO in this catalytic system. O acted as a promoter in the whole process of NO and Hg removal. However, HO showed an inhibiting effect on Hg and NO removal over V-Ce/AC catalysts, which may be caused by the competitive adsorption of HO and pollutants (NO and Hg). Additionally, 1 V-8Ce/AC catalyst exhibited high stability ( E = 87.6%, E = 82.84%) after 72 h reaction in SCR atmosphere at 150 °C.
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http://dx.doi.org/10.1021/acs.est.8b07122DOI Listing
May 2019

[Analysis of cytogenetic characteristics and fertility of carriers of complex chromosomal rearrangements from Henan region].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2019 Feb;36(2):161-164

Genetic and Prenatal Diagnosis Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.

Objective: To explore cytogenetic characteristics and fertility of carriers of complex chromosome rearrangements (CCR) from Henan region.

Methods: G-banded karyotyping analysis was carried out on peripheral blood lymphocytes derived from 160 601 patients with reproductive abnormalities. Relevant literature was retrieved from domestic and overseas databases. Cytogenetic characteristics and clinical data of CCR carriers were analyzed.

Results: Twenty-seven CCR carriers were identified among the 160 601 patients. In addition, 6 cases were identified from the database research. Among the 33 CCR carriers, there were 17 three- and four-way exchange cases (51.5%), 10 double two-way exchange cases (30.3%), and 6 unusual CCRs (18.2%). Infertility was noted in 14 (42.4%) of the CCR carries. A total of 38 pregnancies were achieved in the remaining 19 cases (57.6%), among which spontaneous abortions or embryonic losses have occurred in 89.5% (34/38), multiple congenital abnormalities have occurred in 7.9% (3/38), while phenotypically normal offspring have occurred in 2.6% (1/38). Chromosomes 1, 11, 2, 4, 5 and 12 were more frequently involved, with their breakpoints occurred more than 3 times at 1p22, 11q23, 12p13 and 22q11.

Conclusion: CCR carriers are at a higher risk for abnormal pregnancies. Even for those with normal pregnancy, prenatal diagnosis should be provided. Chromosomes and their breakpoints involved in CCR may affect the fertility of CCR carriers. Analyzing the types of CCR and involved chromosomes and breakpoints can facilitate genetic counseling for CCR carriers.
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http://dx.doi.org/10.3760/cma.j.issn.1003-9406.2019.02.017DOI Listing
February 2019

Oligoethyleneoxy-Modified Tc-Labeled β-Amyloid Imaging Probes with Improved Brain Pharmacokinetics for Single-Photon Emission Computed Tomography.

J Med Chem 2018 02 18;61(3):1330-1339. Epub 2018 Jan 18.

Key Laboratory of Radiopharmaceuticals, Ministry of Education, Beijing Normal University , Beijing 100875, China.

An oligoethyleneoxy linker was introduced for conjugation between Tc/Re-bis(aminoethanethiol) (BAT) and β-amyloid (Aβ) binding scaffolds. Rhenium complexes exhibited high to moderate binding affinity to Aβ aggregates and efficient fluorescent staining to Aβ plaques in brain tissue. After radiolabeling, the Tc-labeled probes revealed improved brain pharmacokinetics in normal ICR mice. Probe [Tc]15 with potent binding affinity (K = 13.4 nM) and the highest initial brain uptake (2.10% ID/g at 2 min) in normal ICR mice was evaluated further. In vitro autoradiography showed specific labeling of Aβ plaques by [Tc]15 in transgenic (Tg) mouse brain tissue. Ex vivo autoradiography further demonstrated its efficient labeling of Aβ plaques in a living Tg mouse. In vivo single photon emission computed tomography (SPECT)/CT imaging in six rhesus monkeys revealed remarkably improved brain uptakes (1.94-2.63% ID within 20 min) of [Tc]15, making it highly potential to be used in humans for Aβ plaques imaging in the brain.
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http://dx.doi.org/10.1021/acs.jmedchem.7b01834DOI Listing
February 2018

Tc-Labeled 2-Arylbenzothiazoles: Aβ Imaging Probes with Favorable Brain Pharmacokinetics for Single-Photon Emission Computed Tomography.

Bioconjug Chem 2016 Oct 7;27(10):2493-2504. Epub 2016 Oct 7.

Key Laboratory of Radiopharmaceuticals, Ministry of Education, Beijing Normal University , Beijing 100875, China.

A series of 2-arylbenzothiazole derivatives conjugated with bis(aminoethanethiol) (BAT) chelating groups were designed and synthesized. A competitive binding assay-based screening was used to select seven rhenium complexes with potent binding affinity toward Aβ aggregates (K < 50 nM) for Tc labeling and further evaluation. The Tc-labeled probes showed good affinity and specificity to Aβ plaques in Tg mouse brain tissue in in vitro autoradiography studies. Moreover, [Tc]14b exhibited favorable brain pharmacokinetics in normal mice (2.11% ID/g at 2 min and 0.62% ID/g at 60 min). Ex vivo autoradiography revealed extensive labeling of Aβ plaques by [Tc]14b in the brain of Tg mice. Furthermore, we performed the first single-photon emission computed tomography (SPECT) imaging study in nonhuman primates with Tc-labeled Aβ probes. The semiquantitative data showed that [Tc]14b penetrated the brains of rhesus monkeys. These results indicate that [Tc]14b could be utilized as a SPECT imaging probe for Aβ plaques.
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http://dx.doi.org/10.1021/acs.bioconjchem.6b00444DOI Listing
October 2016

Influence of calcination temperature on the properties of titanium oxide sulfur recovery catalysts.

J Nanosci Nanotechnol 2014 Sep;14(9):7181-8

To study the effect of calcination temperatures on the sulfur recovery catalysts, titanium oxide (TiO2), as sulfur recovery catalysts, were treated at four calcination temperatures of 300 degrees C, 500 degrees C, 700 degrees C and 900 degrees C. The structure of the catalysts were characterized by X-ray powder diffraction (XRD), Raman, transmission electron microscopy (TEM), Scanning electron microscopy (SEM), temperature thermogravimetry (TG) and differential scanning calorimetry (DSC). The results showed that with the calcination temperature increasing, the particle size of the TiO2 catalysts increases but the surface area and total pore volume decrease. The extent of reduction was more serious after calcination at 500 degrees C. Rutile phase were formed at calcination temperature about 700 degrees C. On the basis of these results, a scheme for the change of TiO2 with increasing calcination temperatures was proposed. The Claus catalytic activity of the TiO2 catalysts was evaluated in the traditional conditions. It was found that the Claus catalytic activity, which decreased a little when the calcination temperature was no more than 500 degrees C but much once the calcination temperature was more than 500 degrees C, was not only related to the surface area and pore volume, but also the phase of the TiO2. The activity of rutile was less than the anatase and a possible reaction pathway to reveal this mechanism was proposed.
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http://dx.doi.org/10.1166/jnn.2014.8953DOI Listing
September 2014

A compound-based computational approach for the accurate determination of hot spots.

Protein Sci 2013 Aug 3;22(8):1060-70. Epub 2013 Jul 3.

The College of Computer Science and Technology, Jilin University, Changchun, Jilin, China.

A plethora of both experimental and computational methods have been proposed in the past 20 years for the identification of hot spots at a protein-protein interface. The experimental determination of a protein-protein complex followed by alanine scanning mutagenesis, though able to determine hot spots with much precision, is expensive and has no guarantee of success while the accuracy of the current computational methods for hot-spot identification remains low. Here, we present a novel structure-based computational approach that accurately determines hot spots through docking into a set of proteins homologous to only one of the two interacting partners of a compound capable of disrupting the protein-protein interaction (PPI). This approach has been applied to identify the hot spots of human activin receptor type II (ActRII) critical for its binding toward Cripto-I. The subsequent experimental confirmation of the computationally identified hot spots portends a potentially accurate method for hot-spot determination in silico given a compound capable of disrupting the PPI in question. The hot spots of human ActRII first reported here may well become the focal points for the design of small molecule drugs that target the PPI. The determination of their interface may have significant biological implications in that it suggests that Cripto-I plays an important role in both activin and nodal signal pathways.
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http://dx.doi.org/10.1002/pro.2296DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3832042PMC
August 2013
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