Publications by authors named "Yao Zhao"

548 Publications

Polystyrene microplastics exacerbate experimental colitis in mice tightly associated with the occurrence of hepatic inflammation.

Sci Total Environ 2022 Jun 22:156884. Epub 2022 Jun 22.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China.

The potential health effects of microplastics (MPs) have become a public concern due to their ubiquitous in the environment and life. Numerous studies have demonstrated that a high dose of MPs can adversely affect gastrointestinal health. However, few studies have focused on the impact of microplastics on patients' health with respect to gastrointestinal diseases. Inflammatory bowel disease (IBD) has emerged as a global disease with a rapidly increasing incidence. IBD, a specific gastrointestinal illness characterized by acute, chronic inflammation and intestinal barrier dysfunction, might increase sensitivity to MPs exposure. Herein, we investigated the impact and mechanism of PS-MPs on dextran sodium sulfate (DSS)-induced colitis. The results demonstrated that gavage with PS-MPs alone caused minimal effects on the intestinal barrier and liver status of mice. For mice with colitis, additional PS-MPs exposure caused a shorter colon length, aggravated histopathological damage and inflammation, reduced mucus secretion, and increased the colon permeability. Furthermore, PS-MPs exposure also increased the risk of secondary liver injury associated with inflammatory cell infiltration. These findings provide more histopathological evidence and suggest a need for more research on the health risk of MPs for sensitive individuals.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156884DOI Listing
June 2022

L-Glutamine alleviates osteoarthritis by regulating lncRNA-NKILA expression through the TGF-β1/SMAD2/3 signalling pathway.

Clin Sci (Lond) 2022 Jun 22. Epub 2022 Jun 22.

Second Affiliated Hospital of Anhui Medical University, Hefei, China.

Osteoarthritis (OA) is a heterogeneous condition characterised by cartilage degradation, subchondral sclerosis, and osteophyte formation, and accompanied by the generation of pro-inflammatory mediators and degradation of extracellular matrix. The current treatment for early OA is focused on the relief of symptoms, such as pain, but this treatment cannot delay the pathological process. L-Glutamine (L-Gln), which has anti-inflammatory and anti-apoptotic effects, is the most abundant amino acid in human blood. However, its role in OA has not been systematically studied. Therefore, the objective of this work was to explore the therapeutic effect and molecular mechanism of L-Gln on OA. In vitro, we found that L-Gln could upregulate the expression of the long non-coding RNA NKILA, which is regulated by the transforming growth factor-β1/SMAD2/3 pathway, and inhibit the activity of nuclear factor-κB, thereby decreasing the expression of nitric oxide synthase, cyclooxygenase-2, and matrix metalloproteinase-13 (MMP-13). This led to a reduction in the generation of nitrous oxide, prostaglandin E-2, tumour necrosis factor-α, and degradation of the extracellular matrix (i.e. aggrecan and collagen II) in rat OA chondrocytes. Moreover, intragastric administration of L-Gln reduced the degradation of cartilage tissue and expression of MMP-13 in a rat OA model. L-Gln also relieved the clinical symptoms in some patients with early knee joint OA. These findings highlight that L-Gln is a potential therapeutic drug to delay the occurrence and development of OA.
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http://dx.doi.org/10.1042/CS20220082DOI Listing
June 2022

15-Hydroxyprostaglandin Dehydrogenase Is a Predictor of Stroke-Associated Pneumonia.

Front Neurol 2022 26;13:893624. Epub 2022 May 26.

Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, China.

Background And Purpose: Stroke is a serious fatal and disabling disease. Stroke-associated pneumonia (SAP) is the most common complication of stroke, which may further aggravate the stroke. The prevention and early prediction of SAP is a key clinical strategy. 15-hydroxyprostaglandin dehydrogenase (15-PGDH) is involved in pneumonia, while its relationship with SAP has yet to be determined. Therefore, we investigated the predictive value of 15-PGDH for SAP and visualized their relationship.

Methods: Stroke patients were recruited and divided into SAP group and Non-SAP group. Baseline demographic and clinical data were obtained from the medical record system, blood samples were collected to detect relevant variables and 15-PGDH levels. Patient characteristics were compared with a t-test. Binary logistic regression analysis was performed to determine the predictive value of 15-PGDH for SAP. Restricted cubic splines (RCS) were performed to visualize the relationship between 15-PGDH and SAP risk. Finally, the SAP patient characteristics between the severe group and mild group were compared.

Results: 50 patients were enrolled and divided into SAP group ( = 26) and Non-SAP group ( = 24). 15-PGDH in the SAP group was lower than that in the Non-SAP group (0.258 ± 0.275 vs. 0.784 ± 0.615, = 0.025). Binary logistic regression analysis revealed that the lower 15-PGDH, the higher the risk of SAP (OR = 0.04, 95%CI, 0.010-0.157, < 0.001). The RCS model showed the L-shaped relationship between 15-PGDH and SAP.

Conclusions: In stroke patients, serum 15-PGDH is a valuable biomarker for predicting SAP. There is an L-shaped relationship between the level of 15-PGDH and the risk of SAP.
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http://dx.doi.org/10.3389/fneur.2022.893624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202497PMC
May 2022

[Bland-Altman consistency evaluation of blood lipid test results under different storage conditions].

Wei Sheng Yan Jiu 2022 May;51(3):490-496

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Objective: To evaluate the consistency of blood lipid test result under different storage conditions.

Methods: Blood samples from 140 subjects were collected, centrifuged and were divided into five aliquots. One aliquot was used to determine serum total cholesterol(TC), triglyceride(TG), high density lipoprotein cholesterol(HDL-C) and low density lipoprotein cholesterol(LDL-C) immediately, and the other four parts were stored at 4 ℃ for 4 h, 24 h, and frozen at-20 ℃ and-80 ℃ for 30 d, respectively, for the further measurement.

Results: Immediate 0 h detection was compared with 4 ℃ for 4 h and 24 h, -20 ℃and-80 ℃ for 30 d: The difference Bland-Altman method was used to analyze TC and LDL-C data, and the mean TC difference values was 0.00, 0.00, 0.07 and 0.03 mmol/L, respectively. LDL-C difference value was-0.02, -0.04, -0.02 and-0.07 mmol/L, respectively, and 95% of the points were within the clinically acceptable conformance threshold. Ratio Bland-Altman method was used to analyze TG and HDL-C data. The mean TG ratios was 1.01, 1.00, 1.05, 1.05, and the mean HDL-C was 1.01, 1.03, 1.05 and 1.03, respectively. Ninety-five percent of the points were within the clinically acceptable consistency boundary. According to the 2021 Interlaboratory Quality Evaluation Standard of Clinical Laboratory Center of National Health Commission, the Bland-Altman analysis showed good consistency between the test result of different blood samples under different storage conditions and the immediate test result.

Conclusion: In large population epidemiological studies, the results of TC, LDL-C, HDL-C and TG in sera being refrigerated at 4 ℃ for 4 h and 24 h, and frozen at-20 ℃ and-80 ℃ for 30 d, were consistent with the result of immediate detection.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2022.03.024DOI Listing
May 2022

[Determination of organophosphorus pesticide residues in milk of cows and sheep by ultra-high-performance liquid chromatography- tandem mass spectrometry combined with passing type purification method].

Wei Sheng Yan Jiu 2022 May;51(3):483-489

Beijing Center for Disease Control and Prevention, Beijing Key Laboratory for Tracing Technology of Food Poisoning Diagnosis, Beijing 100013, China.

Objective: A method for the rapid determination of dichlorvos, trichlorfon, fenthion, fenthion-sulfone, fenthion-sulfoxide, fenthion-oxon, fenthion-oxon-sulfone, fenthion-oxon-sulfoxide, phoxim, propetamphos, malathion, diazinon and coumaphos 13 common organophosphorus pesticides and their metabolites poison residues in milk of cows and sheep by ultra-high-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) with passing type solid phase extraction(SPE) purification was developed.

Methods: After centrifugation at 4 ℃, the milk was purified by passing type SPE with acetonitrile precipitating protein and determined by UPLC-MS/MS in electrospray positive ion mode(ESI+) and multi-reaction monitoring scanning(MRM), external standard method for quantitative analysis with matrix matching standard curve.

Results: The recoveries of 13 target compounds were between 81.5% and 107.5% and relative standard deviation was between 1.24% and 6.23% at three spiked levels of 5, 10, 20 μg/L. The detection limits of 13 target compounds were between 0.015 and 0.15 μg/L, and the quantitative limits were between 0.05 and 0.50 μg/L. No organophosphorus pesticide residues were detected in 20 samples of cows and sheep milk.

Conclusion: The method has the advantages of good linear independence, low detection limit, high precision and accuracy, and can be used for daily monitoring of milk and related products.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2022.03.023DOI Listing
May 2022

First report of Alfalfa root rot caused by in China.

Plant Dis 2022 Jun 13. Epub 2022 Jun 13.

Nanjing Agricultural University, 70578, College of Grassland Science, Nanjing, Jiangsu, China;

Alfalfa () is the most important perennial forage legume worldwide, with high yields and nutrient quality. Diseases are significant threats to alfalfa and cause substantial yield losses (Fang et al. 2021). In March 2022, a disease with symptoms similar to fusarium wilt was observed in an alfalfa (cultivar Aurora) field in Baima teaching and research base (119°18'07″E, 31°61'47″N) in Nanjing, China. Reddish-brown discoloration of the roots, stele, basal stems and the withering symptoms on the aerial portions are the specific symptoms of fusarium wilt. The disease incidence varied from 3 to 6% in around 0.3 hectares of alfalfa fields surveyed. Fresh tissues from symptomatic alfalfa were cut into pieces (3-5 mm) and surface sterilized with 75% ethanol for 30 seconds, followed by 1% sodium hypochlorite (NaClO) for 120 seconds, and three rinses with sterile distilled water. Tissue pieces were placed on selective potato dextrose agar (PDA) containing 50 mg/L rifampicin and ampicillin. Plates were sealed and incubated at 25°C for 48 to 72 hours. Over 70 tissue fragments plated, 20 isolates from different fragments showed similar colonies. These isolates were purified by single spore isolation and grown on PDA and mung bean medium for morphology identification, molecular identification and pathogenicity test. The colony showed white to pink, abundant, densely aerial mycelium, while its backside was light violet. Macroconidia were hyaline, falcate with septa, and ranged in size from 25.5 to 61.5 × 3.8 to 6.2 μm (n=50). Microconidia were non-septate, hyaline, oval, straight to slightly curved, and were 5.6 to 10.7 × 2.4 to 3.2 μm (n=50). Spherical chlamydospores were 10.6 ± 1.3 μm in size. The rDNA internal transcribed spacers (ITS), RNA polymerase II subunit 2 (RPB2) and translation elongation factor 1-α (TEF) genes were amplified and sequenced (White et al. 1990; Carbone et al. 1999; Wang et al. 2019). Blast analysis showed 99.41% to 100% identity to sequences in the GenBank database (KU341324.1, MN892350.1, MH341219.1). The pathogenicity test was conducted using the dipping method in roots. Fresh hyphae were cut into 3 x 3mm agar plugs from a 7 cm PDA plate and inoculated in 200 mL mung bean medium on a shaker at 160 rpm, 25°C for 5 days. Spores were filtered through a cheese cloth, adjusted to 1 × 106 spores/mL with distilled water and used immediately. Roots in two-week-old alfalfa seedlings were soaked in spore suspension for 30 mins before being transplanted to sterile vermiculate with four repeats, while those in sterile water were used as non-inoculated control. The plants were placed in the green house at 25°C with 16 h of light and 8 h of darkness. The symptoms were observed two weeks following the inoculation. 30% of the seedlings withered, and reddish-brown discoloration symptoms were visible on the roots and stem of the unwithered plant. was isolated from these lesions again, and no disease symptoms were observed in the control plant. has been reported to cause root and stem rot in many plants worldwide, including tobacco (Zhong et al. 2021), maize (Mezzalama et al. 2021; Xi et al. 2019), soybean (Detranaltes et al. 2022) and sugarcane (Wang et al. 2018). Our results show that was the causative agent of alfalfa root rot. To our knowledge, this is the first report of causing root rot in alfalfa. The finding provides insights for disease diagnosis of alfalfa root rot disease and management in the region.
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http://dx.doi.org/10.1094/PDIS-06-22-1335-PDNDOI Listing
June 2022

Nickel-Catalyzed Chemo- and Regioselective Arylcyanation of β,γ-Unsaturated Amides.

Org Lett 2022 Jun 10;24(24):4328-4332. Epub 2022 Jun 10.

Institute of Advanced Synthesis, School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China.

A nickel-catalyzed intermolecular arylcyanation of 8-aminoquinolinyl β,γ-unsaturated amides is reported. The three-component reaction directly afforded diverse β-cyano γ-aryl amides with exclusive chemo- and regioselectivity. The synthetic practicality of this approach is further demonstrated through multigram scale reaction, expanded transformations of the nitrile product, late-stage modification of complex molecules, and direct drug synthesis.
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http://dx.doi.org/10.1021/acs.orglett.2c01360DOI Listing
June 2022

Low-rank fusion convolutional neural network for prediction of remission after stereotactic radiosurgery in patients with acromegaly: a proof-of-concept study.

J Pathol 2022 Jun 3. Epub 2022 Jun 3.

Neurosurgical Institute of Fudan University, Shanghai, PR China.

Artificial intelligence approaches to analyze pathological images (pathomic) for outcome prediction have not been sufficiently considered in the field of pituitary research. A total of 5,504 Hematoxylin & Eosin-stained pathology image tiles from 58 acromegalic patients with a good or poor outcome were integrated with other clinical and genetic information to train a low-rank fusion convolutional neural network (LFCNN). The model was externally validated in 1,536 patches from an external cohort. The primary outcome was the time to the first endocrine remission after SRS. The median time of initial endocrine remission was 43 months [IQR: 13-60 months] after SRS, and the 24-month initial cumulative remission rate was 57.9% [IQR: 46.4-72.3%]. The patient-wise accuracy of the LFCNN model in predicting the primary outcome was 92.9% in the internal test dataset, and the sensitivity and specificity were 87.5% and 100.0%, respectively. The LFCNN model was a strong predictor of initial cumulative remission in the training cohort (HR 9.58, 95% CI 3.89-23.59; p < 0.001) and was higher than that of established prognostic markers. The predictive value of LFCNN model was further validated in an external cohort (HR 9.06, 95% CI 1.14-72.25; p = 0.012). In this proof-of-concept study, clinically and genetically useful prognostic markers were integrated with digital images to predict endocrine outcomes after SRS in patients with active acromegaly. The model considerably outperforms established prognostic markers and can potentially be used by clinicians to improve decision-making regarding adjuvant treatment choices. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/path.5974DOI Listing
June 2022

Honokiol Induces Ferroptosis by Upregulating HMOX1 in Acute Myeloid Leukemia Cells.

Front Pharmacol 2022 11;13:897791. Epub 2022 May 11.

Laboratory for Stem Cell and Regenerative Medicine, Affiliated Hospital of Weifang Medical University, Weifang, China.

Acute myeloid leukemia (AML) is one of the malignant hematological cancers with high mortality. Finding a more effective and readily available treatment is of the utmost importance. Here, we aimed to identify the anti-leukemia effect of a natural small molecule compound honokiol on a panel of AML cell lines, including THP-1, U-937, and SKM-1, and explored honokiol's potential biological pathways and mechanisms. The results showed that honokiol decreased the viability of the targeted AML cells, induced their cell cycle arrest at G0/G1 phase, and inhibited their colony-formation capacity. Honokiol also triggers a noncanonical ferroptosis pathway in THP-1 and U-937 cells by upregulating the level of intracellular lipid peroxide and HMOX1 significantly. Subsequent studies verified that HMOX1 was a critical target in honokiol-induced ferroptosis. These results reveal that honokiol is an effective anti-leukemia agent in AML cell lines and may be a potential ferroptosis activator in AML.
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http://dx.doi.org/10.3389/fphar.2022.897791DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132251PMC
May 2022

The hypothalamic-pituitary-gonad axis in male Cushing's disease before and after curative surgery.

Endocrine 2022 May 31. Epub 2022 May 31.

Department of Endocrinology and Metabolism, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200040, China.

Objective: Gonadal and sexual disturbances are commonly encountered in patients with Cushing's disease. Nevertheless, the prevalence of hypogonadism in male Cushing's disease, the risk factors as well as the recovery time have been scarcely reported. Therefore, we aimed to explore the prevalence of hypogonadism at baseline and its determinants. In addition, the recovery time of hypogonadism and risk factors for unrecovered gonadal axis in male Cushing's disease with biochemical remission were investigated.

Methods: We reviewed medical records of males with Cushing's disease managed between 2010 and 2020. Fifty-two male patients were enrolled according to the criteria. Each case attained biochemical remission after transsphenoidal surgery. Demographic details, clinical features, 24-hour UFC, hormonal profile [serum PRL, FSH, LH, TT, ACTH, cortisol, TT4/FT4, TT3/ FT3, TSH and IGF-1] were measured at baseline and during follow-up. The maximal tumor diameter on MRI was recorded at diagnosis.

Results: Hypogonadotropic hypogonadism was observed in thirty-nine patients (75%) at diagnosis. Total testosterone was negatively correlated with ACTH and 24-hour UFC. Midnight serum ACTH level at diagnosis was significantly associated with hypogonadism after adjusting for confounding factors. Thirty-two (80%) patients achieved eugonadism within 12 months after the surgery, of which twenty-eight (87.5%) achieved eugonadism within 3 months. Seven patients were persistently hypogonadal during the follow-up (≥1 year), mainly due to the hypopituitarism as a complication of the therapies such as surgery.

Conclusion: Hypogonadotropic hypogonadism is frequent in male Cushing's disease, but it is reversible in most cases within one-year follow-up after remission.
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http://dx.doi.org/10.1007/s12020-022-03083-yDOI Listing
May 2022

How I do it? Resection of residual petrous apex chordoma with combined endoscopic endonasal and contralateral transmaxillary approaches.

Acta Neurochir (Wien) 2022 Jul 26;164(7):1967-1972. Epub 2022 May 26.

Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200040, China.

Background: The petrous apex is one of the most challenging areas of the skull base to access.

Method: We present a case of residual petrous apex chordoma posterolateral to the paraclival segment of the internal carotid artery (ICA) resected with combined endoscopic endonasal and contralateral transmaxillary (CTM) approaches, without lateralization of the ICA.

Conclusion: This case demonstrates the value of the CTM corridor in resecting petrous apex lesions that are posterolateral to the paraclival segment of the ICA.
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http://dx.doi.org/10.1007/s00701-022-05243-5DOI Listing
July 2022

Twisting for soft intelligent autonomous robot in unstructured environments.

Proc Natl Acad Sci U S A 2022 May 23;119(22):e2200265119. Epub 2022 May 23.

Department of Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC 27695.

SignificanceAutonomy is crucial for soft robotics that are constructed of soft materials. It remains challenging to create autonomous soft robots that can intelligently interact with and adapt to changing environments without external controls. To do so, it often requires an analogical soft "brain" that integrates on-board sensing, control, computation, and decision-making. Here, we report an autonomous soft robot embodied with physical intelligence for decision-making via adaptive soft body-environment interactions and snap-through instability, without integrated sensing and external controls. This study harnesses physical intelligence as a new paradigm for designing autonomous soft robots that can interact intelligently with their environments, thus potentially reducing the burdens on the conventional integrated sensing, control, computations, and decision-making systems in designing intelligent soft robots.
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http://dx.doi.org/10.1073/pnas.2200265119DOI Listing
May 2022

Family Food Environments and Their Association with Primary and Secondary Students' Food Consumption in Beijing, China: A Cross-Sectional Study.

Nutrients 2022 May 8;14(9). Epub 2022 May 8.

Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.

Family is the most fundamental and proximal context for children, and children's eating behavior occurs mostly in the home or together with family members. With this study, we aimed to evaluate the distribution of family food environment dimensions and their relationship with healthy and unhealthy food consumption in primary and secondary students in order to provide evidence in the Chinese context and explore environmental solutions for improved child nutrition. Multi-stage stratified cluster sampling was used to conduct a cross-sectional survey among students in Beijing, China, from September 2020 to June 2021. Family food environment (FFE) was measured by the validated Family Food Environment Questionnaire for Chinese School-age Children, which was self-administered by the children's caregivers. The students were asked to answer questions about food consumption frequencies in the past 7 days. Binary logistic regression models were used to investigate the relationships between food consumption frequency and FFE, and odds ratios () with 95% confidence intervals () were computed for independent variables. Among the study population, 9686 students in grades 3-12 and their caregivers completed the survey. The mean score of FFE was 65.7 (±8.4) out of a total possible score of 100, with 76.6% of students categorized as relatively healthier according to their FFE score (≥ 60). Compared with the reference group, students in primary schools and those cared for principally by parents were more likely to be in a healthy FFE category ( < 0.05). Daily consumption of fruits and vegetables was reported by 62.6% and 71.6% of students, respectively, and weekly sugared soft drink consumption was reported by 70.9% of the students. Students with a healthier FFE score (≥60) were more likely to consume fruits ( = 1.578, 95% : 1.428~1.744) and vegetables ( = 1.402, 95% : 1.263~1.556) but less likely to consume sugared soft drinks ( = 0.592, 95% : 0.526~0.667). Family food availability ( = 0.137), caregivers' nutritional literacy ( = 0.093), meal practices ( = 0.079) and food rules ( = 0.050) were positively correlated with food consumption behavior ( < 0.05). The students with healthier FFE scores ( = 1.130, 95% : 1.014~1.258) and whose caregiver was obese ( = 2.278, 95% : 1.973~2.631) were more likely to be overweight. The family food environment plays an important role in shaping food consumption in children. Provision of healthy foods instead of unhealthy foods, positive meal practices and food rules, and nutrition education for parents can promote healthy eating in children.
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http://dx.doi.org/10.3390/nu14091970DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105134PMC
May 2022

Self-Reported Sedentary Behavior and Metabolic Syndrome among Children Aged 6-14 Years in Beijing, China.

Nutrients 2022 Apr 29;14(9). Epub 2022 Apr 29.

Beijing Center for Disease Prevention and Control, Beijing 100013, China.

(1) Objective: This study aimed to examine the prevalence of metabolic syndrome (MetS) in children aged 6-14 years in Beijing, and to determine whether sedentary behavior is a risk factor. (2) Methods: Using a multistage stratified cluster random sampling method, 3460 students were selected for the Nutrition and Health Surveillance in Schoolchildren of Beijing (NHSSB). Data on children's sedentary behavior time and MetS indicators were collected using the questionnaires, physical measurements, and laboratory tests. MetS was defined according to the CHN2012 criteria, and logistic regression analysis was used to compare the effects of different sedentary time on MetS and its components. (3) Results: The overall prevalence of MetS among children aged 6-14 in Beijing was 2.4%, and boys, suburban children, and older age were associated with a higher prevalence (χ values were 3.947, 9.982, and 27.463, respectively; < 0.05). In boys, the prevalence rates of abdominal obesity, hyperglycemia, high triglycerides (TG), and low high-density lipoprotein cholesterol (HDL-C) were higher in the high-level sedentary behavior group than those in the low-level sedentary behavior group ( < 0.05); and in girls, the prevalence rates of high TG, low HDL-C, and MetS were higher in the high-level sedentary behavior group than those in the low-level sedentary behavior group ( < 0.05). After adjusting for confounding factors, the multivariate logistic regression results showed that compared with children with low-level sedentary behavior, the risks of abdominal obesity and low HDL-C were higher in boys with high-level sedentary behavior (odds ratio (OR) 1.51, 95% confidence interval (CI) 1.10-2.07, = 0.011; OR 2.25, 95% CI 1.06-4.76, = 0.034, respectively); while the risk of abdominal obesity was higher in girls with medium and high-level sedentary behavior (OR 1.52, 95% CI 1.01-2.27, = 0.043; OR 1.59, 95% CI 1.04-2.43, = 0.032, respectively). (4) Conclusions: Higher sedentary behavior time was related to the higher risk of MetS components among children aged 6-14 in Beijing. Reducing sedentary behavior may be an important method for preventing metabolic diseases.
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http://dx.doi.org/10.3390/nu14091869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103420PMC
April 2022

Recommendation to improve the WHO classification of posterior pituitary tumors as a unique entity: evidence from a large case series.

Endocr Connect 2022 Jun 17;11(6). Epub 2022 Jun 17.

Department of Neurosurgery, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai, China.

Introduction: Most studies reporting posterior pituitary tumors (PPTs) are small case series or single cases.

Methods: Patients with a histological diagnosis of PPT from January 2010 to December 2021 in a tertiary center were identified. We reported clinical symptoms, endocrine assessments, radiological and pathological features, and surgical outcomes of PPTs.

Results: A total of 51 patients (23 males, 51.3 ± 10.3 years old) with PPT were included in this study. Major symptoms were visual defects, headache, and hypopituitarism, while diabetes insipidus was uncommon (9.8%). The typical radiological feature was homogeneous enhancement (84.3%) of a regular-shaped mass on T1 contrast imaging without cystic change, calcification, or cavernous sinus invasion. We achieved gross total resection in 38/51 patients (74.5%). Pathologically, all tumors showed thyroid transcription factor 1 immunoreactivity. Among 29 patients with suprasellar PPTs, postoperative hemorrhage due to tumor residue was encountered in 2/15 cases in the transcranial group and 0/14 in the endoscopy group. Patients with spindle cell oncocytoma (SCO) were more likely to be surgically treated (25% vs 0%, P = 0.018), harbor a higher Ki-67 index (16.7% vs 0% > 5% P = 0.050), and present a lower 2-year recurrence-free survival rate (67.5% vs 90.9%) compared with patients with pituicytoma or granular cell tumor.

Conclusion: PPTs should be considered in the differential diagnosis of patients with sellar and suprasellar masses with a regular lesion with homogeneous enhancement. SCOs had high proliferation activity and risk of recurrence.
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http://dx.doi.org/10.1530/EC-22-0188DOI Listing
June 2022

Corrigendum to "Antiliver Fibrosis Screening of Active Ingredients from L. Seeds via GC-TOF-MS and UHPLC-MS/MS".

Evid Based Complement Alternat Med 2022 30;2022:9818920. Epub 2022 Apr 30.

College of Pharmacy, Xinjiang Medical University, Urumqi 830011, China.

[This corrects the article DOI: 10.1155/2020/8321732.].
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http://dx.doi.org/10.1155/2022/9818920DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078777PMC
April 2022

Transcription factor ASCL1 acts as a novel potential therapeutic target for the treatment of the Cushing's disease.

J Clin Endocrinol Metab 2022 May 6. Epub 2022 May 6.

Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.

Background: The pathogenesis of Cushing's disease (CD) is still not adequately understood despite the identification of somatic driver mutations in USP8, BRAF and USP48. In this multiomics study, we combined RNA sequencing (RNA-seq) with Sanger sequencing to depict transcriptional dysregulation under different gene mutation backgrounds. Furthermore, we evaluated the potential of achaete-scute complex homolog 1 (ASCL1), a pioneer transcription factor, as a novel therapeutic target for treatment of CD and its possible downstream pathway.

Methods: RNA-seq was adopted to investigate the gene expression profile of CD, and Sanger sequencing was adopted to detect gene mutations. Bioinformatics analysis was used to depict transcriptional dysregulation under different gene mutation backgrounds. The function of ASCL1 in hormone secretion, cell proliferation and apoptosis were studied in vitro. The effectiveness of a ASCL1 inhibitor was evaluated in primary CD cells, and the clinical relevance of ASCL1 was examined in 68 patients with CD. RNA-seq in AtT-20 cells upon Ascl1 knockdown combined with published ChIp-seq data and dual luciferase assays were used to explore downstream pathways.

Results: ASCL1 was exclusively overexpressed in USP8-mutant and wild type tumors. Ascl1 promoted adrenocorticotrophin hormone overproduction and tumorigenesis and directly regulated Pomc in AtT-20 cells. A ASCL1 inhibitor presented promising efficacy in both AtT-20 and primary CD cells. ASCL1 overexpression was associated with a larger tumor volume and higher adrenocorticotrophin secretion in patients with CD.

Conclusion: Our findings help to clarify the pathogenesis of CD and suggest that ASCL1 is a potential therapeutic target for treatment of CD.
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http://dx.doi.org/10.1210/clinem/dgac280DOI Listing
May 2022

Latent Low-Rank Representation With Weighted Distance Penalty for Clustering.

IEEE Trans Cybern 2022 May 4;PP. Epub 2022 May 4.

Latent low-rank representation (LatLRR) is a critical self-representation technique that improves low-rank representation (LRR) by using observed and unobserved samples. It can simultaneously learn the low-dimensional structure embedded in the data space and capture the salient features. However, LatLRR ignores the local geometry structure and can be affected by the noise and redundancy in the original data space. To solve the above problems, we propose a latent LRR with weighted distance penalty (LLRRWD) for clustering in this article. First, a weighted distance is proposed to enhance the original Euclidean distance by enlarging the distance among the unconnected samples, which can enhance the discriminitation of the distance among the samples. By leveraging on the weighted distance, a weighted distance penalty is introduced to the LatLRR model to enable the method to preserve both the local geometric information and global information, improving discrimination of the learned affinity matrix. Moreover, a weight matrix is imposed on the sparse error norm to reduce the effect of noise and redundancy. Experimental results based on several benchmark databases show the effectiveness of our method in clustering.
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http://dx.doi.org/10.1109/TCYB.2022.3166545DOI Listing
May 2022

Correction: Nanoarchitectonics of p-type BiSbTe with improved figure of merit introducing PbTe nanoparticles.

RSC Adv 2021 Nov 2;11(61):38717. Epub 2021 Dec 2.

College of Electronic and Information, Qingdao University Qingdao 266071 China

[This corrects the article DOI: 10.1039/D1RA07138F.].
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http://dx.doi.org/10.1039/d1ra90170bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044182PMC
November 2021

Nanoarchitectonics of p-type BiSbTe with improved figure of merit introducing PbTe nanoparticles.

RSC Adv 2021 Nov 12;11(58):36636-36643. Epub 2021 Nov 12.

College of Electronic and Information Engineering, Qingdao University Qingdao 266071 China

BiSbTe (BST) is known to be a unique p-type commercial thermoelectric (TE) alloy used at room temperatures, but its figure of merit () is relatively low for wide industrial applications. To improve its value is vitally important. Here, we show that the incorporation of 0.5 wt% PbTe nanoparticles into BST concurrently causes a large enhancement of power factor (PF) and a significant reduction of lattice thermal conductivity . The increase in PF mainly benefits from the optimization of carrier concentration, maintenance of high carrier mobility and constant rise in Seebeck coefficient. The decrease in can be attributed to the enhanced phonon scattering by the dispersed PbTe nanoparticles and the interfaces between PbTe and the BST matrix by using the Callaway model. Specifically, an ultralow of 0.26 W m K at 429 K is achieved for the composites incorporating 0.5 wt% PbTe nanoinclusions. Consequently, an excellent = 1.6 at 482 K and a high average = 1.38 at 300-500 K are achieved, indicating that incorporation of PbTe in BST is an effective approach to improve its thermoelectric performance.
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http://dx.doi.org/10.1039/d1ra07138fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043475PMC
November 2021

Risk factors of epistaxis after endoscopic endonasal skull base surgeries.

Clin Neurol Neurosurg 2022 06 7;217:107243. Epub 2022 Apr 7.

Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China; Neurosurgical Institute of Fudan University, Shanghai 200040, China; Shanghai Clinical Medical Center of Neurosurgery, Shanghai 200040, China; Shanghai Key Laboratory of Brain Function Restoration and Neural Regeneration, Shanghai 200040, China; Shanghai Pituitary Tumor Center, Shanghai 200040, China; National Center for Neurological Disorders, China. Electronic address:

Background: Epistaxis after endoscopic endonasal skull base surgeries does not typically occur as an accidental circumstance but often results from multiple factors. We aimed to assess the possible risk factors associated with postoperative epistaxis.

Methods: Patients who underwent endoscopic endonasal skull base surgery at Huashan hospital from August 2018 to November 2019 were enrolled in the study. Postoperative epistaxis was defined as severe, persistent, or recurrent arterial nosebleed, which required therapeutic intervention. Patients were divided into the epistaxis and no epistaxis groups. The incidence, clinical characteristics, management, and intraoperative findings were recorded.

Results: A total of 762 patients were included in the study. 20 (2.6%) patients experienced postoperative epistaxis, which happened in a delayed fashion between 6 and 30 postoperative days. Multivariate logistic regression analysis showed that arterial hypertension (OR=3.394, 95%CI: 1.094-10.531, P = 0.034) and preoperative systolic blood pressure (SBP) (OR=1.035, 95%CI: 1.002-1.068) were identified as predictors for postoperative epistaxis. The bleeding sites were identified at the left posterior septal artery (PSA) in 10 (62.5%) cases, the right PSA in 4 (25%) cases, the left palatovaginal artery in 1 (6.25%) case, and the right inferior turbinate branch of the posterior lateral nasal artery in 1 (6.25%) case, respectively. Direct bipolar cauterization was used to stop the bleeding. After definitive treatment, no patients had recurrent epistaxis.

Conclusions: Arterial hypertension and preoperative SBP were associated with postoperative epistaxis after endoscopic endonasal skull base surgery, and the left PSA contributed to the dominant site of bleeding during surgical re-exploration.
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http://dx.doi.org/10.1016/j.clineuro.2022.107243DOI Listing
June 2022

Structural Insight into Molecular Inhibitory Mechanism of InsP on African Swine Fever Virus mRNA-Decapping Enzyme g5Rp.

J Virol 2022 05 28;96(10):e0190521. Epub 2022 Apr 28.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan Universitygrid.13291.38 and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, People's Republic of China.

Removal of 5' cap on cellular mRNAs by the African swine fever virus (ASFV) decapping enzyme g5R protein (g5Rp) is beneficial to viral gene expression during the early stages of infection. As the only nucleoside diphosphate-linked moiety X (Nudix) decapping enzyme encoded in the ASFV genome, g5Rp works in both the degradation of cellular mRNA and the hydrolyzation of the diphosphoinositol polyphosphates. Here, we report the structures of dimeric g5Rp and its complex with inositol hexakisphosphate (InsP). The two g5Rp protomers interact head to head to form a dimer, and the dimeric interface is formed by extensive polar and nonpolar interactions. Each protomer is composed of a unique N-terminal helical domain and a C-terminal classic Nudix domain. As g5Rp is an mRNA-decapping enzyme, we identified key residues, including K, K, K, K, K, R, and K located on the substrate RNA binding interfaces of g5Rp which are important to RNA binding and decapping enzyme activity. Furthermore, the g5Rp-mediated mRNA decapping was inhibited by InsP. The g5Rp-InsP complex structure showed that the InsP molecules occupy the same regions that primarily mediate g5Rp-RNA interaction, elucidating the roles of InsP in the regulation of the viral decapping activity of g5Rp in mRNA degradation. Collectively, these results provide the structural basis of interaction between RNA and g5Rp and highlight the inhibitory mechanism of InsP on mRNA decapping by g5Rp. ASF is a highly contagious hemorrhagic viral disease in domestic pigs which causes high mortality. Currently, there are still no effective vaccines or specific drugs available against this particular virus. The protein g5Rp is the only viral mRNA-decapping enzyme, playing an essential role in the machinery assembly of mRNA regulation and translation initiation. In this study, we solved the crystal structures of g5Rp dimer and complex with InsP. Structure-based mutagenesis studies revealed critical residues involved in a candidate RNA binding region, which also play pivotal roles in complex with InsP. Notably, InsP can inhibit g5Rp activity by competitively blocking the binding of substrate mRNA to the enzyme. Our structure-function studies provide the basis for potential anti-ASFV inhibitor designs targeting the critical enzyme.
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http://dx.doi.org/10.1128/jvi.01905-21DOI Listing
May 2022

Heat Shock Proteins and Ferroptosis.

Front Cell Dev Biol 2022 11;10:864635. Epub 2022 Apr 11.

Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China.

Ferroptosis is a new form of regulatory cell death named by Dixon in 2012, which is characterized by the accumulation of lipid peroxides and iron ions. Molecular chaperones are a class of evolutionarily conserved proteins in the cytoplasm. They recognize and bind incompletely folded or assembled proteins to help them fold, transport or prevent their aggregation, but they themselves do not participate in the formation of final products. As the largest number of molecular chaperones, heat shock proteins can be divided into five families: HSP110 (HSPH), HSP90 (HSPC), HSP70 (HSPA), HSP40 (DNAJ) and small heat shock proteins (HSPB). Different heat shock proteins play different roles in promoting or inhibiting ferroptosis in different diseases. It is known that ferroptosis is participated in tumors, nervous system diseases, renal injury and ischemia-reperfusion injury. However, there are few reviews about the relationship of heat shock proteins and ferroptosis. In this study, we systematically summarize the roles of heat shock proteins in the occurrence of ferroptosis, and predict the possible mechanisms of different families of heat shock proteins in the development of ferroptosis.
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http://dx.doi.org/10.3389/fcell.2022.864635DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035830PMC
April 2022

Male spiders avoid sexual cannibalism with a catapult mechanism.

Curr Biol 2022 04;32(8):R354-R355

Department of Biological Sciences, National University of Singapore, Singapore 117543, Republic of Singapore. Electronic address:

In the animal world, numerous mechanisms have been described that allow for extremely fast actions or reactions via the slow storage of energy, typically in elastic structures, which is then nearly instantly released, similar to the operation of a catapult. Many of these mechanisms are employed for prey capture or for predator avoidance; however, such superfast actions have not yet been reported as a means to dodge sexual cannibalism. Here, we unveil a novel mechanism in a communal orb-weaving spider Philoponella prominens (Uloboridae) (Figure S1), whereby males undertake a split-second catapult action immediately after mating, thereby fleeing their partner (Video S1). We demonstrate that males achieve their superfast action (up to 88.2 cm/s) by extending the tibia-metatarsus joint of their first leg pair via hydraulic pressure in a joint that is known to lack extensor muscles in spiders. This rapid expansion greatly reduces the likelihood of the male being sexually cannibalized.
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http://dx.doi.org/10.1016/j.cub.2022.03.051DOI Listing
April 2022

JMJD1C-regulated lipid synthesis contributes to the maintenance of -rearranged acute myeloid leukemia.

Leuk Lymphoma 2022 Apr 25:1-12. Epub 2022 Apr 25.

Laboratory for Stem Cell and Regenerative Medicine, Affiliated Hospital of Weifang Medical University, Weifang, China.

rearranged acute myeloid leukemia (r AML) predicts a poor prognosis. Histone demethylase JMJD1C is a potential druggable target of r AML. However, little is known about how JMJD1C contributes to r AML. Here we found that JMJD1C regulates lipid synthesis-associated genes including FADS2, SCD in r AML cells. Lipid synthesis-associated protein FABP5 was identified as a specific interacting protein of JMJD1C and binds to the jumonji domain of JMJD1C. FABP5 also regulates JMJD1C mRNA and protein expression. JDI-10, a small molecular inhibitor of JMJD1C identified by us, represses r AML cells, induces apoptosis, and decreases JMJD1C-regulated lipid synthesis genes. Moreover, JDI-10 mediated suppression of r AML cells can be rescued by palmitic acid, oleic acid, or recombinant FABP5. In summary, we identified that JMJD1C-regulated lipid synthesis contributes to the maintenance of r AML. Lipid synthesis repression may represent a new direction for the treatment of r AML.
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http://dx.doi.org/10.1080/10428194.2022.2068004DOI Listing
April 2022

A nitric oxide burst at the shoot apex triggers a heat-responsive pathway in Arabidopsis.

Nat Plants 2022 04 18;8(4):434-450. Epub 2022 Apr 18.

National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai, China.

When confronted with heat stress, plants depend on the timely activation of cellular defences to survive by perceiving the rising temperature. However, how plants sense heat at the whole-plant level has remained unanswered. Here we demonstrate that shoot apical nitric oxide (NO) bursting under heat stress as a signal triggers cellular heat responses at the whole-plant level on the basis of our studies mainly using live-imaging of transgenic plants harbouring pHsfA2::LUC, micrografting, NO accumulation mutants and liquid chromatography-tandem mass spectrometry analysis in Arabidopsis. Furthermore, we validate that S-nitrosylation of the trihelix transcription factor GT-1 by S-nitrosoglutathione promotes its binding to NO-responsive elements in the HsfA2 promoter and that loss of function of GT-1 disrupts the activation of HsfA2 and heat tolerance, revealing that GT-1 is the long-sought mediator linking signal perception to the activation of cellular heat responses. These findings uncover a heat-responsive mechanism that determines the timing and execution of cellular heat responses at the whole-plant level.
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http://dx.doi.org/10.1038/s41477-022-01135-9DOI Listing
April 2022

Serum Phosphopeptides Profiling for Colorectal Cancer Diagnosis Using Liquid Chromatography-Mass Spectrometry.

Rapid Commun Mass Spectrom 2022 Apr 13:e9316. Epub 2022 Apr 13.

Beijing National Laboratory for Molecular Sciences; National Centre for Mass spectrometry in Beijing; CAS Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, PR China.

Rationale: The identification and evaluation of novel biomarkers is essential to clinical diagnosis and prognosis of colorectal cancer (CRC). Serum phosphopeptides have been recognized as potential signature pool for cancers, therefore, we aim to profile the expression of serum phosphopeptides and to evaluate their feasibility in CRC diagnosis.

Methods: We conducted the characterization and absolute quantification of endogenous phosphopeptides in sera by liquid chromatography-mass spectrometry (LC-MS) analysis in combination with enrichment of phosphopeptides by ZrAs-Fe O @SiO nanoparticles and use of deuterium-labeled standards. Differentially expressed analysis of four phosphopeptides was performed and generated a two phosphopeptide-based biomarker L by logistic regression analysis, where L is equal to (5.85-5.13×[F3] - 3.57×[F4]), and [F3] and [F4] are the concentration in sera of phosphopeptidies DpSGEGDFLAEGGGVR and ADpSGEGDFLAEGGGVR, respectively.

Results: The L values showed significantly difference in CRC cases compared with controls, and yielded a specificity of 100%, leading to correct classification of 56 (93%) out of 60 CRC patients, including 12 (92.3%) of 13 CRC cases in stage I. Double-blind validation allowed 97.5% CRC cases discriminated accurately.

Conclusions: The L value was firstly verified to be a potential biomarker for CRC diagnosis, and may expand our view in underlying mechanisms for CRC.
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http://dx.doi.org/10.1002/rcm.9316DOI Listing
April 2022

Decreasing arsenic accumulation but promoting arsenate biotransformation in Microcystis aeruginosa regulated by nano-FeO.

Environ Sci Pollut Res Int 2022 Apr 9. Epub 2022 Apr 9.

Xiamen Mata Ecology Co., Ltd, Xiamen, 361021, China.

Iron oxide nanoparticles (nano-FeO) widely distribute in waters with low toxicity to aquatic organisms. But it is unclear for nano-FeO to affect the fate of coexisting arsenic (As) with its bioaccumulation and biotransformation. In this study, we thus mainly investigated arsenate (As(V)) toxicity, uptake kinetics, biotransformation and subcellular distribution in Microcystis aeruginosa influenced by nano-FeO. The results showed that M. aeruginosa was more sensitive to As(V) associated with nano-FeO. Due to the exaggerated increase of efflux rate constants of As compared with the uptake rate constants in algal cells affected by different levels of nano-FeO, the As(V) bioconcentration factor decreased with nano-FeO increasing correspondingly, indicating that As bioaccumulation was diminished by nano-FeO The decreased As accumulation in M. aeruginosa could be supported by the evidential As(V) sequestration through high adsorption of nano-FeO, which resulted in decreasing free As level for algae uptake in media. Meanwhile, As subcellular distribution was adjusted by nano-FeO with decreasing in cell walls and rising in cytoplasmic organelles compared with nano-FeO free. As(V) reduction and methylation were enhanced with increasing nano-FeO, stimulating by its sensitivity to the interaction of nano-FeO and As(V) as well as the rising level of As in cytoplasmic organelles of this algae. It is confirmed by the higher relative gene expression levels of arsC and arsM in elevated nano-FeO. Accordingly, it is highlighted to be deserved more attention that the changing behavior of As(V) by nano-FeO that reduce As bioaccumulation and accelerate its biotransformation in algae in As contaminated water.
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http://dx.doi.org/10.1007/s11356-022-20042-2DOI Listing
April 2022

Network pharmacology-based strategy to investigate pharmacological mechanisms of Andrographolide for treatment of vascular cognitive impairment.

Int Immunopharmacol 2022 Jul 6;108:108756. Epub 2022 Apr 6.

Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. Electronic address:

Vascular cognitive impairment (VCI) is the second most common form of dementia. Andrographolide (Andro) shows potential effects in anti-inflammation, anti-oxidative stress, and anti-apoptosis. We have obtained 48 potential genes related to the effect of Andro on VCI through network pharmacology analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were used to reveal significant enriched pathway of potential genes, and the mitogen-activated protein kinase (MAPK) pathway was screened out. To verify the results of network pharmacology, we tested the effects of Andro in VCI model induced by bilateral common carotid artery occlusion (BCCAO) surgery. The results showed that Andro treatment ameliorated the cognitive impairment induced by BCCAO. Immunohistochemistry study revealed that Andro could reduce neuronal damage and activation of microglia in the cortex and hippocampus in BCCAO rats. To test the MAPK pathway changes, we analyzed the expression of JNK, p38 and ERK and found that Andro reduced the levels of phosphorylated-ERK (p-ERK) and phosphorylated-p38 (p-p38) in BCCAO rats. In conclusion, Andro could improve neuronal survival, reduce neuroinflammation and ameliorate cognitive impairment in VCI. The underlying mechanisms of Andro treatment may be through the inhibition of MAPK pathway.
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http://dx.doi.org/10.1016/j.intimp.2022.108756DOI Listing
July 2022

Exemplar-Based, Semantic Guided Zero-Shot Visual Recognition.

IEEE Trans Image Process 2022 11;31:3056-3065. Epub 2022 Apr 11.

Zero-shot recognition has been a hot topic in recent years. Since no direct supervision is available, researchers use semantic information as the bridge instead. However, most zero-shot recognition methods jointly model images on the class level without considering the distinctive character of each image. To solve this problem, in this paper, we propose a novel exemplar-based, semantic guided zero-shot recognition method (EBSG). Both visual and semantic information of each image is used. We train visual sub-model to separate each image from the other images of different classes. We also train semantic sub-model to separate this image from the other images described with different semantics. We concatenate the outputs of visual and semantic sub-models to represent images. Image classification model is then learned by measuring visual similarity and semantic consistency of both source and target images. We conduct zero-shot recognition experiments on four widely used datasets. Experimental results show the effectiveness of the proposed EBSG method.
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http://dx.doi.org/10.1109/TIP.2021.3120319DOI Listing
April 2022
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