Publications by authors named "Yao Zhang"

1,424 Publications

  • Page 1 of 1

Facile synthesis of core\shell [email protected](Hb) and its application for organic wastewater treatment.

Environ Res 2021 Jul 30:111796. Epub 2021 Jul 30.

College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China; College of Life Sciences, Northeast Agricultural University, Harbin, China. Electronic address:

Treatment of organic wastewater is a challenging task. Biological techniques using biocatalysts have shown their benefits in organic wastewater treatment. In this research, a novel biocatalyst was developed by encapsulation of Fe3O4 microspheres and haemoglobin (Hb) with mesoporous silica, named [email protected](Hb). [email protected](Hb) exhibited typical mesoporous characteristics (mesoporous silica), magnetic feature (Fe3O4) and peroxidase activity (Hb). The results showed that the immobilization of Hb into [email protected] did not affect its activity. In addition, [email protected](Hb) exhibited a higher efficiency in the peroxidation of aromatic compounds than free Hb. The peroxidase activity of the synthesized biocatalyst was estimated to be 120 Ug, which was almost four times greater than that of previously reported immobilized Hb. Also, the Km of [email protected](Hb) was similar to that of the free Hb and it was estimated to be 4.3 × 10 μM, indicating that the activity of the Hb in the immobilized enzyme was not affected after immobilization. The immobilized enzyme was also found to be stable, recyclable and reusable. Taken together, these results indicate that the [email protected](Hb) has good potential to be used for treating organic wastewater containing aromatic compounds. The magnetically separable novel biocatalyst developed in this study provided not only a more suitable microenvironment for retaining the activity of Hb, but also demonstrated enhanced stability and activity under unfavorable conditions.
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http://dx.doi.org/10.1016/j.envres.2021.111796DOI Listing
July 2021

The regulatory pathways of distinct flowering characteristics in Chinese jujube.

Hortic Res 2020 Aug 1;7(1):123. Epub 2020 Aug 1.

Research Center of Chinese Jujube, Hebei Agricultural University, Baoding, 071000, China.

Flowering is the most important event in higher plants. Compared to most fruit tree species, Chinese jujube (Ziziphus jujuba Mill.), the most important member of the large, diverse Rhamnaceae family and a leading dry fruit-producing species, has unique characteristics that include a short juvenile phase and extremely fast flower bud differentiation. However, the distinct mechanism of flowering regulation in Chinese jujube is still unclear. The morphological and cytological development period of jujube flowering was first investigated, and the crucial developmental stages were defined. Flower bud differentiation in Chinese jujube took only approximately 11-13 days, which is a distinct characteristic of perennial fruit trees. Afterward, 44 genes related to six flowering pathways were identified in the jujube genome and were found to be randomly distributed among 11 of the 12 chromosomes. Tissue-specific and spatiotemporal expression patterns showed that all these genes were expressed in the flowers. Overall, photoperiod-related genes were highly expressed during flower bud differentiation. These genes were also positively responsive to photoperiod regulation and phase change processes, indicating that photoperiod- related genes play crucial roles in jujube flower bud differentiation. Under protected cultivation, ZjPIF4, a temperature-related gene, was expressed in the early stages of flowering and responded to increasing temperatures. Moreover, STRING analysis and yeast two-hybrid screening indicated that photoperiod-related (ZjCO) and temperature-related (ZjPIF4) proteins could interact with ZjFT, the key protein involved in the determination of flowering time, indicating crosstalk between photoperiod-related pathways and ambient temperature-related pathways in jujube. This study is the first report to comprehensively analyze the flowering pathways in Chinese jujube and revealed that photoperiod-related and ambient temperature-related pathways are the main mechanisms regulating the distinct flowering process and that members of the ZjPHY family (ZjPIF4, ZjFT, and ZjCO5) are the key factors involved in the regulatory network. These results will increase our understanding of the molecular and genetic mechanisms of flowering in Chinese jujube and provide meaningful clues for the flowering regulation of other fruit tree species.
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http://dx.doi.org/10.1038/s41438-020-00344-7DOI Listing
August 2020

Publisher Correction: Silica nanoparticles as pesticide against insects of different feeding types and their non-target attraction of predators.

Sci Rep 2021 Jul 30;11(1):15899. Epub 2021 Jul 30.

Entomological Laboratory, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.

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http://dx.doi.org/10.1038/s41598-021-95389-6DOI Listing
July 2021

Molecular-Scale Chemical Imaging of the Orientation of an On-Surface Coordination Complex by Tip-Enhanced Raman Spectroscopy.

J Am Chem Soc 2021 Jul 30. Epub 2021 Jul 30.

Department of Chemistry and Applied Biosciences, ETH Zurich, Vladimir-Prelog-Weg 3, Zurich CH-8093, Switzerland.

Metal-organic coordination structures at interfaces play an essential role in many biological and chemical systems. Understanding the molecular specificity, orientation, and spatial distribution of the coordination complexes at the nanometer scale is of great importance for effective molecular engineering of nanostructures and fabrication of functional devices with controllable properties. However, fundamental properties of such coordination systems are still rarely studied directly. In this work, we present a spectroscopic approach on the basis of tip-enhanced Raman spectroscopy (TERS) to investigate cobalt(II) tetraphenyl-porphyrine coordination species on the scale of a single molecule under ambient conditions. Coordination species anchored on gold surfaces modified with pyridine thiol self-assembled monolayers can be spectroscopically distinguished and mapped with . 2 nm resolution. In addition, in combination with density functional theory simulations, the adsorption configuration and molecular orientation of the coordination complexes are also revealed using TERS imaging.
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http://dx.doi.org/10.1021/jacs.1c06366DOI Listing
July 2021

In-situ nanospectroscopic imaging of plasmon-induced two-dimensional [4+4]-cycloaddition polymerization on Au(111).

Nat Commun 2021 Jul 27;12(1):4557. Epub 2021 Jul 27.

Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland.

Plasmon-induced chemical reactions (PICRs) have recently become promising approaches for highly efficient light-chemical energy conversion. However, an in-depth understanding of their mechanisms at the nanoscale still remains challenging. Here, we present an in-situ investigation by tip-enhanced Raman spectroscopy (TERS) imaging of the plasmon-induced [4+4]-cycloaddition polymerization within anthracene-based monomer monolayers physisorbed on Au(111), and complement the experimental results with density functional theory (DFT) calculations. This two-dimensional (2D) polymerization can be flexibly triggered and manipulated by the hot carriers, and be monitored simultaneously by TERS in real time and space. TERS imaging provides direct evidence for covalent bond formation with ca. 3.7 nm spatial resolution under ambient conditions. Combined with DFT calculations, the TERS results demonstrate that the lateral polymerization on Au(111) occurs by a hot electron tunneling mechanism, and crosslinks form via a self-stimulating growth mechanism. We show that TERS is promising to be plasmon-induced nanolithography for organic 2D materials.
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http://dx.doi.org/10.1038/s41467-021-24856-5DOI Listing
July 2021

AbdomenCT-1K: Is Abdominal Organ Segmentation A Solved Problem.

IEEE Trans Pattern Anal Mach Intell 2021 Jul 27;PP. Epub 2021 Jul 27.

With the unprecedented developments in deep learning, automatic segmentation of main abdominal organs seems to be a solved problem as state-of-the-art (SOTA) methods have achieved comparable results with inter-rater variability on many benchmark datasets. However, most of the existing abdominal datasets only contain single-center, single-phase, single-vendor, or single-disease cases, and it is unclear whether the excellent performance can generalize on diverse datasets. This paper presents a large and diverse abdominal CT organ segmentation dataset, termed AbdomenCT-1K, with more than 1000 (1K) CT scans from 12 medical centers, including multi-phase, multi-vendor, and multi-disease cases. Furthermore, we conduct a large-scale study for liver, kidney, spleen, and pancreas segmentation and reveal the unsolved segmentation problems of the SOTA methods, such as the limited generalization ability on distinct medical centers, phases, and unseen diseases. To advance the unsolved problems, we further build four organ segmentation benchmarks for fully supervised, semi-supervised, weakly supervised, and continual learning, which are currently challenging and active research topics. Accordingly, we develop a simple and effective method for each benchmark, which can be used as out-of-the-box methods and strong baselines. We believe the AbdomenCT-1K dataset will promote future in-depth research towards clinical applicable abdominal organ segmentation methods.
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http://dx.doi.org/10.1109/TPAMI.2021.3100536DOI Listing
July 2021

Nitrifiers drive successions of particulate organic matter and microbial community composition in a starved macrocosm.

Environ Int 2021 Jul 23;157:106776. Epub 2021 Jul 23.

State Key Laboratory of Marine Environmental Science and College of Ocean and Earth Sciences, Xiamen University, Xiamen 361101, China. Electronic address:

Organic carbon produced by nitrifiers plays an important role in maintaining the microbial metabolism in the aphotic ocean layer with carbon and energy scarcity. However, the contribution of nitrifiers to organic carbon processing remains unclear. To explore how nitrification impacts the material cycle in the starved ecosystem, we set up an ultra-large volume, long-term incubation experiment. Seawater collected from the Halifax coastal ocean was pumped into the Aquatron Tower Tank located at Dalhousie University, Canada, and was incubated under dark conditions for 73 days. The results indicated that the relative abundance of nitrifiers increased and nitrification was strengthened in the dark system where energy and organic carbon were scarce. The importance of nitrogenous compounds in particulate materials increased over the course of the incubation. Correlation analysis showed that the relative abundances of nitrifiers and particulate organic compounds containing nitrogen were significantly and positively correlated. Furthermore, network analysis suggested that metabolic processes related to nitrogenous and aromatic compounds are most important to particle associated bacteria. This study suggests that the nitrifiers could produce a series of organic compounds that result in the alteration of organic matter composition by promoting the degradation of recalcitrant aromatic compounds, which has important implications for organic matter processing in the starved dark ecosystem.
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http://dx.doi.org/10.1016/j.envint.2021.106776DOI Listing
July 2021

Microarray analysis of genes with differential expression of m6A methylation in lung cancer.

Biosci Rep 2021 Jul 26. Epub 2021 Jul 26.

Xijing Hospital, Xi'an, China.

Purpose: N6-methyladenosine (m6A) is among the most abundant mRNA modifications in eukaryote. The aim of this study was to investigate function of m6A mRNA methylation in lung cancer and the underlying mechanism.

Methods: Microarray analysis was performed to detect the differences in RNA expression between cancerous and adjacent non-cancerous tissue samples. The target mRNAs were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. Hierarchical clustering of RNAs was conducted to identify distinct m6A methylation or expression patterns between the samples.

Results: In this study, some differentially expressed genes (DEGs) of mRNAs were identified, including up-regulated SPP1 and down-regulated pRB. Functional enrichment analysis revealed that while differential hypermethylation was related to cell cycle, intracellular part and protein binding, the main pathway involved herpes simplex virus 1 infection related to down-regulated AKT, Araf1 and BCL2A1. In the meantime, sexual reproduction, cohesin complex and portein C-terminus binding was functionally linked to differential hypomethylation, while fluid shear stress and atherosclerosis were identified as the main pathways related to up-regulated GST and CNP.

Conclusions: We showed that lung cancer development involved differential expression of SPP1 and pRB mRNA, as well as m6A mRNA methylation in AKT, APAF1, BCL2A1, GST and CNP genes.
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http://dx.doi.org/10.1042/BSR20210523DOI Listing
July 2021

Mifepristone (RU486) inhibits dietary lipid digestion by antagonizing the role of glucocorticoid receptor on lipase transcription.

iScience 2021 Jun 12;24(6):102507. Epub 2021 May 12.

Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, 6B, Shixun Bldg, 1239 Siping Road, Yangpu District, Shanghai, 20092, China.

Lipid digestion and absorption are tightly regulated to cope with metabolic demands among tissues. How these processes are coordinated is not well characterized. Here, we found that mifepristone (RU486) prevents lipid digestion both in flies and mice. In flies, RU486 administration suppresses lipid digestion by transcriptional downregulating in guts. Similarly, intestinal lipid uptake in mice was also suppressed by RU486 through the glucocorticoid receptor (GR). Further studies showed that the pancreatic lipase is a direct transcriptional target of GR in pancreas tissues. Glucocorticoid levels in mice fed a high fat diet (HFD) are significantly lower than those fed on a conventional diet, and RU486 administration inhibits HFD-induced obesity both in mice and flies. Our findings identified a novel mechanism of RU486 functions as a GR antagonist systematically regulating lipid metabolism, providing new insight on the role of Glucocorticoid/GR in Cushing disease, diabetes, and other related metabolic syndromes.
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http://dx.doi.org/10.1016/j.isci.2021.102507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257970PMC
June 2021

Development of a Mobile Application of Internet-Based Support Program on Parenting Outcomes for Primiparous Women.

Int J Environ Res Public Health 2021 Jul 9;18(14). Epub 2021 Jul 9.

Health Science Centre, Shenzhen University, Shenzhen 518060, China.

Primiparous women usually experience various parenting problems after childbirth that have negative effects on the well-being of mothers and infants. Although e-Support technology could provide an innovative and easily accessible intervention approach, mobile-phone interventions remain limited for Chinese primiparous women. Therefore, a new mobile application (APP) called the "Internet-based Support Program" ("ISP") was designed, incorporating the self-efficacy theory and the social-exchange theory for Chinese first-time mothers to improve their levels of maternal self-efficacy (MSE), social support, and satisfaction, as well as to reduce their postpartum depression symptoms. The research was conducted to develop and optimize the "ISP" APP for new mothers via a theory-, evidence-, and person-based approach. Five modules of "learning forum", "communication forum", "ask-the-expert forum", "baby home forum", and "reminder forum" were included in the APP to meet various parenting needs of first-time mothers; and its contents and functions were validated by the experts and primiparous women. The majority of participants gave positive feedback on the APP's perceived ease of use and usefulness. The "ISP" APP was the first designed for Chinese primiparous women, and a multicenter randomized controlled trial (RCT) will be conducted to measure its effectiveness on parenting outcomes.
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http://dx.doi.org/10.3390/ijerph18147354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308101PMC
July 2021

FER Regulated by miR-206 Promotes Hepatocellular Carcinoma Progression NF-κB Signaling.

Front Oncol 2021 5;11:683878. Epub 2021 Jul 5.

Hepatobiliary Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Objectives: Feline sarcoma-related protein (FER) is known to play a critical regulatory role in several carcinomas. However, the exact biological function of FER in hepatocellular carcinoma (HCC) still needs to be investigated. The primary objective of this research was to investigate the unknown function and molecular mechanisms of FER in HCC.

Materials And Methods: The expression level of FER in HCC tissue samples and cells was examined by RT-qPCR, immunohistochemistry and western blot. Cellular and animal experiments were used to explore the effect of FER on the proliferative and metastatic capacities of HCC cells. The crosstalk between FER and NF-κB signaling was explored by western blot. The upstream factors that regulate FER were evaluated through dual-luciferase experiments and western blot assays.

Results: FER was overexpressed in HCC specimens and HCC cell lines. FER expression levels were positively associated with unfavorable clinicopathological characteristics. The higher the expression of FER was, the worse the overall survival of HCC patients was. The results of loss-of-function and gain-of-function experiments indicated that knockdown of FER decreased, while overexpression of FER increased, the proliferation, invasion and metastasis of HCC cells and . Mechanistically, we found that FER activated the NF-κB signaling pathway and stimulated epithelial-to-mesenchymal transition (EMT). We also found that FER was directly regulated by miR-206, and the downregulation of miR-206 was associated with proliferation and metastatic progression in HCC.

Conclusions: The present research was the first to reveal that a decrease in miR-206 levels results in an increase in FER expression in HCC, leading to enhanced cell growth and metastatic abilities activation of the NF-κB signaling pathway.
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http://dx.doi.org/10.3389/fonc.2021.683878DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289706PMC
July 2021

A clinical nomogram incorporating salivary level and oral hygiene index for predicting colorectal cancer.

Ann Transl Med 2021 May;9(9):754

Division of Gastroenterology and Hepatology, Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, State Key Laboratory for Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Institute of Digestive Disease, Shanghai, China.

Background: Emerging evidence demonstrates that the salivary microbiome could serve as a biomarker for various diseases. To date, the oral microbiome's role in the diagnosis of colorectal cancer (CRC) has not been fully elucidated. We aimed to illustrate the salivary microbiome's role in diagnosing and predicting the risk of CRC.

Methods: We collected preoperational saliva from 237 patients [95 healthy controls (HCs) and 142 CRC patients] who underwent surgical resections or colorectal endoscopy in Renji Hospital from January 2018 to January 2020. Clinical demographics, comorbidities, and oral health conditions were obtained from medical records or questionnaires. Salivary microbial biomarkers were detected using quantitative polymerase chain reaction (qPCR) after DNA extraction. Multivariate logistic regression analysis was employed to analyze the risk factors for CRC. A predictive model for the risk of developing CRC was constructed based on logistic regression analysis. Predictive accuracy was internally validated by bootstrap resampling. A clinical nomogram was constructed to visualize the predictive model.

Results: Logistic regression analysis demonstrated that the risk factors associated with CRC included age at diagnosis, male sex, poor oral hygiene, and relative salivary abundance. The predictive model had good discriminative (0.866) and calibration abilities (0.834) after bias correction.

Conclusions: The model based on age, sex, oral hygiene index (OHI), and the salivary level, which is visualized by a clinical nomogram, can predict the risk of CRC. Developing good oral hygiene habits might reduce the risk of CRC.
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http://dx.doi.org/10.21037/atm-20-8168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246182PMC
May 2021

Health-Related Quality of Life in Patients With Different Diseases Measured With the EQ-5D-5L: A Systematic Review.

Front Public Health 2021 29;9:675523. Epub 2021 Jun 29.

School of International Pharmaceutical Business, China Pharmaceutical University, Nanjing, China.

The EQ-5D-5L is a generic preference-based questionnaire developed by the EuroQol Group to measure health-related quality of life (HRQoL) in 2005. Since its development, it has been increasingly applied in populations with various diseases and has been found to have good reliability and sensitivity. This study aimed to summarize the health utility elicited from EQ-5D-5L for patients with different diseases in cross-sectional studies worldwide. Web of Science, MEDLINE, EMBASE, and the Cochrane Library were searched from January 1, 2012, to October 31, 2019. Cross-sectional studies reporting utility values measured with the EQ-5D-5L in patients with any specific disease were eligible. The language was limited to English. Reference lists of the retrieved studies were manually searched to identify more studies that met the inclusion criteria. Methodological quality was assessed with the Agency for Health Research and Quality (AHRQ) checklist. In addition, meta-analyses were performed for utility values of any specific disease reported in three or more studies. In total, 9,400 records were identified, and 98 studies met the inclusion criteria. In the included studies, 50 different diseases and 98,085 patients were analyzed. Thirty-five studies involving seven different diseases were included in meta-analyses. The health utility ranged from 0.31 to 0.99 for diabetes mellitus [meta-analysis random-effect model (REM): 0.83, (95% CI = 0.77-0.90); fixed-effect model (FEM): 0.93 (95% CI = 0.93-0.93)]; from 0.62 to 0.90 for neoplasms [REM: 0.75 (95% CI = 0.68-0.82); FEM: 0.80 (95% CI = 0.78-0.81)]; from 0.56 to 0.85 for cardiovascular disease [REM: 0.77 (95% CI = 0.75-0.79); FEM: 0.76 (95% CI = 0.75-0.76)]; from 0.31 to 0.78 for multiple sclerosis [REM: 0.56 (95% CI = 0.47-0.66); FEM: 0.67 (95% CI = 0.66-0.68)]; from 0.68 to 0.79 for chronic obstructive pulmonary disease [REM: 0.75 (95% CI = 0.71-0.80); FEM: 0.76 (95% CI = 0.75-0.77)] from 0.65 to 0.90 for HIV infection [REM: 0.84 (95% CI = 0.80-0.88); FEM: 0.81 (95% CI = 0.80-0.82)]; from 0.37 to 0.89 for chronic kidney disease [REM: 0.70 (95% CI = 0.48-0.92; FEM: 0.76 (95% CI = 0.74-0.78)]. EQ-5D-5L is one of the most widely used preference-based measures of HRQoL in patients with different diseases worldwide. The variation of utility values for the same disease was influenced by the characteristics of patients, the living environment, and the EQ-5D-5L value set. : https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42020158694.
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http://dx.doi.org/10.3389/fpubh.2021.675523DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275935PMC
June 2021

Silica nanoparticles as pesticide against insects of different feeding types and their non-target attraction of predators.

Sci Rep 2021 Jul 14;11(1):14484. Epub 2021 Jul 14.

Entomological Laboratory, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.

The agricultural use of silica (SiO) nanoparticles (NPs) has the potential to control insect pests while the safety and tritrophic effects on plants and beneficial natural enemies remains unknown. Here, we evaluate the effects of silica NPs on insect pests with different feeding niches, natural enemies, and a plant. Silica NPs were applied at different concentrations (75-425 mg/L) on field-cultivated faba bean and soybean for two growing seasons. The faba bean pests, the cowpea aphid Aphis craccivora and the American serpentine leafminer Liriomyza trifolii, and the soybean pest, the cotton leafworm Spodoptera littoralis, were monitored along with their associated predators. Additional laboratory experiments were performed to test the effects of silica NPs on the growth of faba bean seedlings and to determine whether the rove beetle Paederus fuscipes is attracted to cotton leafworm-infested soybean treated with silica NPs. In the field experiments, silica NPs reduced the populations of all three insect pests and their associated predators, including rove beetles, as the concentration of silica NPs increased. In soybean fields, however, the total number of predators initially increased after applying the lowest concentration. An olfactometer-based choice test found that rove beetles were more likely to move towards an herbivore-infested plant treated with silica NPs than to a water-treated control, suggesting that silica NPs enhance the attraction of natural enemies via herbivore-induced plant volatiles. In the laboratory, while silica NPs inhibited the development of faba bean roots at 400 mg/L, they did not affect germination percentage, germination time, shoot length, or vigor index compared to the control.
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http://dx.doi.org/10.1038/s41598-021-93518-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280210PMC
July 2021

Adipose Stem Cell-Derived Exosomes Ameliorate Chronic Rotator Cuff Tendinopathy by Regulating Macrophage Polarization: From a Mouse Model to a Study in Human Tissue.

Am J Sports Med 2021 Jul;49(9):2321-2331

Jinshan Branch of Shanghai Sixth People's Hospital, Shanghai, China.

Background: Chronic rotator cuff (RC) tendinopathy is one of the most prevalent causes of shoulder pain. Growing evidence suggests that macrophages play a significant role in the proinflammatory response, resolution of inflammation, and tissue healing of tendinopathy. In particular, enhancement of M2 macrophage (M2φ) activity contributes to the accelerated healing of tendinopathy. Therefore, a treatment that enhances M2φ polarization would be useful for patients with this common musculoskeletal disorder.

Purpose: To investigate whether adipose stem cell-derived exosomes (ASC-Exos) enhance M2φ polarization and ameliorate chronic RC tendinopathy.

Study Design: Controlled laboratory study.

Methods: First, we compared the effects of ASC-Exos on polarization of mouse bone marrow-derived macrophages between a classically activated phenotype (M1φ) and an alternatively activated phenotype (M2φ) in vitro. In total, 72 C57BL/6 mice were assigned to normal cage activity (n = 24) or 5 weeks of treadmill overuse (n = 48). The supraspinatus tendon of each treadmill overuse mouse was treated with ASC-Exos (n = 24) or saline (n = 24). Histological and biomechanical outcomes were assessed 4 weeks after treatment. Finally, tissue samples from human patients with RC tendinopathy were obtained to assay the effect of ASC-Exos on the M1φ/M2φ balance in tissue-resident macrophages.

Results: ASC-Exos inhibited M1φ polarization and augmented M2φ polarization in vitro and in vivo. Mice in the ASC-Exos group showed less severe pathological changes than those in the saline group, including less cellular infiltration, disorganization of collagen, and ground substance deposition. The modified Bonar score of the ASC-Exos group (mean ± SD, 7.68 ± 1.03) was significantly lower than that of the saline group (9.81 ± 0.96; < .05). Furthermore, the maximum failure load was significantly higher in the ASC-Exos group than in the saline group (4.23 ± 0.66 N vs 3.86 ± 0.65 N; < .05), as was stiffness (3.38 ± 0.34 N/m vs 2.68 ± 0.49 N/m; < .05).

Conclusion: ASC-Exos-mediated polarization balance of M1φ/M2φ contributes to the amelioration of chronic RC tendinopathy. Regulation of the M1φ/M2φ balance could be a new target for the treatment of chronic RC tendinopathy.

Clinical Relevance: Administration of ASC-Exos is a cell-free approach that may become a novel treatment option for chronic RC tendinopathy and should be explored further.
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http://dx.doi.org/10.1177/03635465211020010DOI Listing
July 2021

Quantitative proteomic and phosphoproteomic analysis of chicken skeletal muscle during embryonic development.

Anim Biotechnol 2021 Jul 8:1-12. Epub 2021 Jul 8.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, P. R. China.

Skeletal muscle also plays a vital role in regulating the movement energy storage and health of metabolism. In order to investigate the expression profile of protein and phosphor-proteins in chicken skeletal muscle during embryonic development, we performed phosphor-proteomics analysis by label-free and TiO enrichment strategy in chicken leg muscle tissues of at embryonic age embryo day 7(E7), E12, E17 and 3-day post-hatch (D3). The study led to the identification of 4332 proteins in the proteome and 1043 phosphorylation modification sites in the phosphorylated proteome, corresponding to 718 proteins (FC ≥ 2 or FC ≤ 0.5 and  < 0.05). The DEP-associated biological processes were involved in Focal adhesion, Glycolysis/gluconeogenesis, Arginine and proline metabolism by KEGG analysis. PPI analyses revealed that these DEPs TNNC1, TNNC2, TNNT2, TNNT3 and phosphorylated DEPs MYLPF interacted with involved pathways. Integrative analysis of proteome and phosphoproteome data found 324 common proteins, corresponding to 521 modification sites and Focal adhesion was the only pathway significantly enriched. These results provide a basis for further understanding the proteome and phosphoproteome and their regulatory biochemical pathways during the development of embryonic chicken skeletal muscle.
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http://dx.doi.org/10.1080/10495398.2021.1941071DOI Listing
July 2021

SARS-CoV-2 infection in the mouse olfactory system.

Cell Discov 2021 Jul 6;7(1):49. Epub 2021 Jul 6.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.

SARS-CoV-2 infection causes a wide spectrum of clinical manifestations in humans, and olfactory dysfunction is one of the most predictive and common symptoms in COVID-19 patients. However, the underlying mechanism by which SARS-CoV-2 infection leads to olfactory disorders remains elusive. Herein, we demonstrate that intranasal inoculation with SARS-CoV-2 induces robust viral replication in the olfactory epithelium (OE), not the olfactory bulb (OB), resulting in transient olfactory dysfunction in humanized ACE2 (hACE2) mice. The sustentacular cells and Bowman's gland cells in the OE were identified as the major target cells of SARS-CoV-2 before invasion into olfactory sensory neurons (OSNs). Remarkably, SARS-CoV-2 infection triggers massive cell death and immune cell infiltration and directly impairs the uniformity of the OE structure. Combined transcriptomic and quantitative proteomic analyses revealed the induction of antiviral and inflammatory responses, as well as the downregulation of olfactory receptor (OR) genes in the OE from the infected animals. Overall, our mouse model recapitulates olfactory dysfunction in COVID-19 patients and provides critical clues for understanding the physiological basis for extrapulmonary manifestations of COVID-19.
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http://dx.doi.org/10.1038/s41421-021-00290-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260584PMC
July 2021

Diagnosis and staging of multiple myeloma using serum-based laser-induced breakdown spectroscopy combined with machine learning methods.

Biomed Opt Express 2021 Jun 21;12(6):3584-3596. Epub 2021 May 21.

Institute of Opto-electronics, Harbin Institute of Technology, 2 Yikuang Street, 150080 Harbin, China.

Diagnosis and staging of multiple myeloma (MM) have been achieved using serum-based laser-induced breakdown spectroscopy (LIBS) in combination with machine learning methods. 130 cases of serum samples collected from registered MM patients in different progressive stages and healthy controls were deposited onto standard quantitative filter papers and ablated with a Q-switched Nd:YAG laser. Emissions of Ca, Na, K, Mg, C, H, O, N and CN were selected for malignancy diagnosis and staging. Multivariate statistics and machine learning methods, including principal component analysis (PCA), k-nearest neighbor (kNN), support vector machine (SVM) and artificial neural network (ANN) classifiers, were used to build the discrimination models. The performances of the classifiers were optimized via 10-fold cross-validation and evaluated in terms of accuracy, sensitivity, specificity, and area under the receiver operating characteristic curves (AUC). The kNN, SVM and ANN classifiers achieved comparable discrimination performances with accuracies of over 90% for both diagnosis and staging of MM. For diagnosis of MM, the classifiers achieved performances with AUC of ∼0.970, sensitivity of ∼0.930 and specificity of ∼0.910; for staging of MM, the corresponding values were AUC of ∼0.970, sensitivity of ∼0.910 and specificity of ∼0.930. These results show that the serum-based LIBS in combination with machine learning methods can serve as a fast, less invasive, cost-effective, and robust technique for diagnosis and staging of human malignancies.
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http://dx.doi.org/10.1364/BOE.421333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221939PMC
June 2021

CCL24 Protects Renal Function by Controlling Inflammation in Podocytes.

Dis Markers 2021 16;2021:8837825. Epub 2021 Jun 16.

Department of Endocrinology and Genetic Metabolism, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu 241002, China.

Diabetic nephropathy (DN) is one of the most lethal complications of diabetes mellitus with chronic inflammation. We have examined the role of the inflammatory chemokine CCL24 in DN. We observed that serum levels of CCL24 were significantly elevated in patients with DN. Not only that, the expression of CCL24 was significantly increased in the kidneys of DN mice. The kidney of DN mice showed increased renal fibrosis and inflammation. We characterized an in vitro podocyte cell model with high glucose. Western blot analysis showed that expression of CCL24 was significantly increased under high-glucose conditions. Stimulation with high glucose (35 mmol/L) resulted in an increase in CCL24 expression in the first 48 hours but changed little after 72 hours. Moreover, with glucose stimulation, the level of podocyte fibrosis gradually increased, the expression of the proinflammatory cytokine IL-1 was upregulated, and the expression of the glucose transporter GLUT4, involved in the insulin signal regulation pathway, also increased. It is suggested that CCL24 is involved in the pathogenesis of DN. In order to study the specific role of CCL24 in this process, we used the CRISPR-Cas9 technique to knock out CCL24 expression in podocytes. Compared with the control group, the podocyte inflammatory response induced by high glucose after CCL24 knockout was significantly increased. These results suggest that CCL24 plays a role in the development of early DN by exerting an anti-inflammatory effect, at least, in podocytes.
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http://dx.doi.org/10.1155/2021/8837825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221868PMC
June 2021

Self-expanding metal stent insertion by colorectal surgeons using a two-person approach colonoscopy without fluoroscopic monitoring in the management of acute colorectal obstruction: a 14-year experience.

World J Surg Oncol 2021 Jul 2;19(1):194. Epub 2021 Jul 2.

Department of Colorectal Surgery, Changhai Hospital, Naval Military Medical University, Shanghai, 200433, China.

Background: Placement of a self-expanding metal stent (SEMS) in patients presenting with an acute colorectal obstruction (ACO) may obviate emergency surgery (ES), potentially effectively palliating incurable tumors, acting as a bridge to surgery (BTS) in patients with operable or potentially operable tumors and achieving effective decompression of other ACO. We present our experience with SEMS insertion by colorectal surgeons without fluoroscopic monitoring for ACO especially for acute malignant colorectal obstruction (AMCO) for nearly a 14-year period (2007-2020).

Aim: To explore the safety and effectiveness of SEMS insertion in the management of ACO by colorectal surgeons using a two-person approach colonoscopy without fluoroscopic monitoring.

Methods: We reviewed the medical records of patients retrospectively to identify all patients presenting to our unit with ACO especially with AMCO who had stenting carried out to achieve colonic decompression. All 434 procedures were performed by colorectal surgeons using a two-person approach colonoscopy without fluoroscopic monitoring.

Results: The overall technique success rate and clinic success rate by SEMS insertion were 428/434 (98.6%) and 412/434 (94.9%). The overall incidence of complications by SEMS insertion was 19/434 (4.4%). The complications included clinical perforation (6/434, 1.4%); stent migration (2/434, 0.5%), 1 of which re-stent; stent detachment (fell off) (3/434, 0.7%), none of them with re-stent; stool impaction (6/434, 1.4%), 1 of which re-stent; and abdominal or anal pain (2/434, 0.5%). There was no hemorrhage in any of the 434 patients.

Conclusions: SEMS insertion is a relatively safe and effective technique for colonic decompression in dealing with ACO as either a BTS or as a palliative measure. It is also a solution to other causes of ACO such as recurrent tumor, benign diseases, or extra-luminal compression. Therefore, ES was largely avoided.
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http://dx.doi.org/10.1186/s12957-021-02309-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254346PMC
July 2021

Novel PVA-Based Microspheres Co-Loaded with Photothermal Transforming Agent and Chemotherapeutic for Colorectal Cancer Treatment.

Pharmaceutics 2021 Jun 29;13(7). Epub 2021 Jun 29.

Department of Gastroenterology, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, No.197, Rui Jin Er Road, Shanghai 200025, China.

Background: We previously designed an electrospinning chitosan (CS) nanofiber-based carrier, using polyvinyl alcohol (PVA) as an adjuvant to deliver doxorubicin (DOX) and MoS nanosheets for postoperative tumor re-occurrence inhibition. However, owing to that the nanofibrous mat is un-injectable, this composite nanofiber is far from being clinically applicable.

Materials And Methods: Via modulating the electrospray parameters, polyvinyl alcohol (PVA) beads string doped with DOX and MoS (PVA/MoS/DOX microspheres) were prepared, which were further crosslinked with glutaraldehyde to obtain the water-stability.

Results: Under the 808-nm laser irradiation, MoS nanosheets rendered the prepared PVA/MoS/DOX microspheres an excellent light-to-heat conversion performance with η of 23.2%. Besides, the heat generated by near-infrared laser irradiation can improve the effect of chemotherapy by promoting the release rate of DOX. HT29 cell and tumor-bearing nude mice were used to systematically study the combined tumor treatment efficiency of composite nanospheres.

Conclusion: PVA/MoS/DOX nanospheres have excellent photothermal effect and chemotherapy effect, which can completely suppress the tumor recurrence. Therefore, the PVA/MoS/DOX nanospheres are anticipated to find potential applications in the treatment of local colorectal cancer.
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http://dx.doi.org/10.3390/pharmaceutics13070984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309159PMC
June 2021

Combination of Quercetin and Vitamin E Supplementation Promotes Yolk Precursor Synthesis and Follicle Development in Aging Breeder Hens via Liver-Blood-Ovary Signal Axis.

Animals (Basel) 2021 Jun 28;11(7). Epub 2021 Jun 28.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.

The fertility of female animals is negatively correlated with increasing chronological age. In aging broiler breeder hens, there is a decline in the functionality of the ovary and liver accompanied by hormonal or endocrine changes, a reduction in antioxidant capacity, and a decrease in folliculogenesis. Therefore, improving the reproductive function in aging breeder hens using dietary strategies is of great concern to the poultry breeder. This study evaluated the capacity of dietary quercetin (Q), vitamin E (VE), and their combination (Q + VE) to promote follicle development and attenuate organ inflammation by improving the antioxidant capacity of aging breeder hens. In this study, 400 broiler breeder hens (Tianfu broilers breeder hens, 435 days old) were allotted into four groups (100 birds each) with four replicates each (25 birds each). They were fed diets containing Q (0.4 g/kg), VE (0.2 g/kg), Q + VE (0.4 g/kg + 0.2 g/kg), and a basal diet for 10 weeks. The results showed that Q + VE improved the organ characteristics ( < 0.05), and also that Q + VE showed protective effects on the liver against injury, as well as increasing the antioxidant capacity of the liver, serum, and ovary ( < 0.05). Furthermore, liver lipid synthesis was increased remarkably, as indicated by the changes in triglyceride levels in hens fed Q + VE ( < 0.05). Levels of E2, FSH, and LH, their receptors, and mRNAs related to yolk precursor synthesis were increased by the Q + VE ( < 0.05). Therefore, the combination of quercetin and vitamin E synergistically promotes and regulates the transportation and exchange of synthetic substances among the liver-blood-ovary alliances to ensure the synchronous development and functional coordination between the liver and ovary in aging breeder hens.
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http://dx.doi.org/10.3390/ani11071915DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8300405PMC
June 2021

Double-pass pre-chirp managed amplification with high gain and high average power.

Opt Lett 2021 Jul;46(13):3115-3118

We demonstrate, to the best of our knowledge, the first double-pass pre-chirp managed fiber amplifier. The double-pass fiber amplifier exhibits high gain allowing us to amplify chirped picosecond pulses from 20 mW to 113 W in a rod-type Yb-fiber corresponding to 38 dB gain. We study the dependence of static mode degradation (SMD) on the nonlinear phase shift (NPS) accumulated by the amplified pulse. Our results indicate that a larger nonlinear phase shift results in stronger nonlinear polarization evolution of the fundamental mode and leads to a lower threshold for SMD. After optimization, our pre-chirp managed amplifier seeded by 80 mW pulses delivers 102 W amplified power from the main output. The amplified pulses are compressed to 37 (55) fs with 90 (100) W average power by a grating pair (chirped mirrors). The double-pass configuration significantly simplifies the implementation of pre-chirp managed fiber amplifiers leading to an extremely compact system.
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http://dx.doi.org/10.1364/OL.428066DOI Listing
July 2021

Ring finger protein 19A is overexpressed in non-small cell lung cancer and mediates p53 ubiquitin-degradation to promote cancer growth.

J Cell Mol Med 2021 Jun 29. Epub 2021 Jun 29.

Department of Pathology, College of Basic Medical Sciences and First Affiliated Hospital, China Medical University, Shenyang, China.

The expression pattern, biological functions and the related mechanisms of the ring finger protein 19A (RNF19A) in non-small cell lung cancer (NSCLC) remain poorly understood. This study aimed to explore the role of RNF19A, as well as the underlying potential mechanism, in the development of NSCLC. Here, we found that RNF19A was overexpressed in NSCLC tissues, and RNF19A expression in NSCLC tissue samples was associated with NSCLC carcinogenesis and poor outcome. RNF19A promoted the proliferation of NSCLC cells and inhibited apoptosis. RNF19A reduced p53, p21 and BAX expression and induced Cyclin D1, CDK4, CDK6 and BCL2 expression. The inhibitory effect of RNF19A knockdown on proliferation was partially rescued by p53 silencing. RNF19A interacted with p53, shortened p53 half-life and mediated p53 ubiquitin-degradation. Collectively, we suggest that RNF19A plays a critical oncogenic role in lung carcinogenesis by disrupting the function of p53. RNF19A may serve as a new biomarker and/or target for NSCLC management.
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http://dx.doi.org/10.1111/jcmm.16674DOI Listing
June 2021

A new triarylindanone and a new isobenzofuranone derivative from .

Nat Prod Res 2021 Jun 28:1-6. Epub 2021 Jun 28.

Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, PR China.

A new triarylindanone, namely selagindanone A (), and a new isobenzofuranone (), 3,4-bis(4-hydroxyphenyl)isobenzofuran-1(3)-one, were isolated from . Their structures were elucidated by comprehensive spectroscopic and mass spectrometric analyses, including 1 D-, 2 D-NMR and HR-ESI-MS. Compound possesses a unique structural feature of triaryl-substituted in the skeleton of 1-indanone. In addition, compound showed weak cytotoxicity against human hepatocellular carcinoma SMMC-7721 and HepG2 cell lines.
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http://dx.doi.org/10.1080/14786419.2021.1938042DOI Listing
June 2021

Vacancy-Mediated Hydrogen Spillover Improving Hydrogen Storage Properties and Air Stability of Metal Hydrides.

Small 2021 Jun 24:e2100852. Epub 2021 Jun 24.

College of Materials Science and Engineering, Jiangsu Collaborative Innovation Centre for Advanced Inorganic Function Composites, Nanjing Tech University, 30 South Puzhu Road, Nanjing, PR, 211816, China.

Hydrogen storage in metal hydrides is a promising solution for sustainable and clean energy carriers. Although Mg-based metal hydrides are considered as potential hydrogen storage media, severe surface passivation has limited their industrial application. In this study, a simple, cheap, and efficient method is proposed to produce highly reactive and air-stable bulk Mg-Ni-based hydrides by rapid treatment with water for 3 min. The nickel-decorated Mg(OH) nanosheets formed in situ during hydrolysis can provide a pathway for hydrogen desorption via vacancy-mediated hydrogen spillover, as revealed by density functional theory calculations, thereby significantly decreasing the peak dehydrogenation temperature by 108.2 °C. Moreover, water-activated hydrides can be stored under ambient conditions without surface decay and activity loss, exhibiting excellent air stability, which can be attributed to the chemical stability of the surface layer. The results provide alternative insights into the design of highly active, air-stable metal hydrides with low cost and promote the industrial application of hydrogen energy.
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http://dx.doi.org/10.1002/smll.202100852DOI Listing
June 2021

Malnutrition delayed wound healing after tooth extraction by HMGB1-related prolonged inflammation.

Int Immunopharmacol 2021 Jul 24;96:107772. Epub 2021 May 24.

Department of Pathophysiology - Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan. Electronic address:

Malnutrition causes prolonged inflammation, resulting in delayed wound healing. High mobility group box-1 (HMGB1) is a damage-associated molecular pattern that is present in the nuclei of macrophages and is secreted into the extracellular milieu in response to stimuli. It stimulates the production of interleukin-1β (IL-1β) through the receptors for advanced glycation end products (RAGE), inducing an inflammatory response, which is an essential response to initiate wound healing. We hypothesized that malnutrition may interfere with this cascade, causing abnormal inflammation and ultimately delaying wound healing. We used tooth-extracted mice with malnutrition fed with low-casein diet for two weeks. On days 3 and 7 after tooth extraction, the wound tissue was histologically observed and analyzed for several factors in the inflammation-regeneration lineage, including IL-1β, mesenchymal stem cells, myeloperoxidase activity, HMGB1, macrophage polarization, and adenosine 5-triphosphate (ATP). On day 7, delayed wound healing was observed with the following findings under malnutrition conditions: decreased mRNA expression of genes for regeneration and mesenchymal stem cell (MSC) accumulation, an obvious increase in myeloperoxidase and IL-1β mRNA expression, an increase in HMGB1 levels, and an increase in ATP concentration in tissues with elevated proportion of M2 macrophages. These results suggest that the significantly increased secretion of HMGB1 associated with the upregulated production of ATP and IL-1β secretion via the RAGE pathway may interfere with the resolution of inflammation and wound healing under the state of malnutrition.
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http://dx.doi.org/10.1016/j.intimp.2021.107772DOI Listing
July 2021

Evaluation of ultrasonic indexes and exercise endurance application of diuretic mixture combined with metoprolol in the treatment of senile chronic heart failure.

Minerva Surg 2021 Jun 23. Epub 2021 Jun 23.

Department of Ultrasound, Ditan Hospital, Capital Medical University, Beijing, China.

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http://dx.doi.org/10.23736/S2724-5691.21.08954-1DOI Listing
June 2021

The Mediating Effect of Positive Illness Cognitions on Experiential Avoidance and Quality of Life in Breast Cancer Patients.

Asia Pac J Oncol Nurs 2021 Jul-Aug;8(4):427-432. Epub 2021 Apr 24.

School of Nursing, Xuzhou Medical University, Department of Nursing Administration, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.

Objective: Illness cognition plays an essential role during physical, psychological, and social adjustment among patients with cancer. The present study aims to explore the mediating effects of positive illness cognition on experiential avoidance and quality of life (QOL) in this population.

Methods: Between August 2017 and June 2019, we recruited 312 patients with breast cancer in the treatment period from a general tertiary hospital's breast department using convenience sampling. We used the Illness Cognition Questionnaire, the Acceptance and Action Questionnaire II, and the Functional Assessment of Cancer Therapy-Breast Scale.

Results: The mean score of QOL was 93.39 (SD: 18.60) for patients with breast cancer. Positive illness cognition was closely related to the QOL and experiential avoidance. Experiential avoidance significantly negatively correlated with QOL ( = -0.59, < 0.01) and positive illness cognition ( = -0.60, < 0.01), while positive illness cognition significantly positively correlated with QOL ( = 0.82, < 0.01). Positive illness cognition had a mediating effect between experiential avoidance and QOL (effect size: -0.56), accounting for 87.14% of the total effect.

Conclusions: The QOL was low in Chinese patients with breast cancer. Positive illness cognition had a mediating effect between experiential avoidance and QOL. Caregivers should indirectly improve patients' QOL with breast cancer by improving their positive illness cognition levels.
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http://dx.doi.org/10.4103/apjon.apjon-20102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186393PMC
April 2021

Quantitative muscle MRI biomarkers in Duchenne muscular dystrophy: cross-sectional correlations with age and functional tests.

Biomark Med 2021 Jun 22;15(10):761-773. Epub 2021 Jun 22.

Pfizer Inc, Cambridge, MA 02139, USA.

Using baseline data from a clinical trial of domagrozumab in Duchenne muscular dystrophy, we evaluated the correlation between functional measures and quantitative MRI assessments of thigh muscle. Analysis included timed functional tests, knee extension/strength and North Star Ambulatory Assessment. Patients (n = 120) underwent examinations of one thigh, with MRI sequences to enable measurements of muscle volume (MV), MV index, mean T2 relaxation time via T2-mapping and fat fraction. MV was moderately correlated with strength assessments. MV index, fat fraction and T2-mapping measures had moderate correlations ( ∼ 0.5) to all functional tests, North Star Ambulatory Assessment and age. The moderate correlation between functional tests, age and baseline MRI measures supports MRI as a biomarker in Duchenne muscular dystrophy clinical trials. Trial registration: ClinicalTrials.gov, NCT02310763; registered 4 November 2014.
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http://dx.doi.org/10.2217/bmm-2020-0801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253163PMC
June 2021
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