Publications by authors named "Yao Xiao"

698 Publications

Mechanism of Liver Regeneration During ALPPS.

Front Cell Dev Biol 2022 8;10:916286. Epub 2022 Jun 8.

Department of General Surgery, Xiangya Hospital, Central South University, Changsha, China.

Liver cancer is one of the most lethal malignant tumors in the world, and surgical resection is the main treatment for liver cancer. Liver failure due to insufficient residual liver volume is a fatal complication after hepatectomy. How to effectively increase the residual liver volume after hepatectomy and improve the safety of hepatectomy has always been a problem to be solved in liver surgery. Associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) effectively reduces the occurrence of liver failure due to insufficient residual liver volume after hepatectomy, thereby increasing the probability of radical resection by inducing rapid proliferation of residual liver tissue. However, the molecular mechanism of residual liver tissue regeneration after primary ALPPS (combined liver partition and portal vein ligation) remains unclear. Here, we found that lots of circular RNAs (circRNAs) are upregulated after ALPPS in pig liver cells; then, we identified the orthologous circRNA in humans and pigs to detect their function in liver regeneration. The results showed that loss of circ-0067724 and circ-0016213 could suppress liver cell proliferation. Together, these findings suggest that circ-0067724 and circ-0016213 play an important role in liver cell proliferation, and this may help us to find new strategies to promote liver regeneration.
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http://dx.doi.org/10.3389/fcell.2022.916286DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213876PMC
June 2022

Optimization Model of Financial Market Portfolio Using Artificial Fish Swarm Model and Uniform Distribution.

Authors:
Yao Xiao

Comput Intell Neurosci 2022 15;2022:7483454. Epub 2022 Jun 15.

Chongqing Industry Polytechnic College, Chongqing 400000, China.

The central issue in finance is how to select a portfolio in the financial market. The traditional artificial fish swarm algorithm (AFSA) is optimized in this paper, and the improved AFSA is used to solve the portfolio model. This model generates a uniform distribution operator using uniform distribution and combines it with the basic fish swarm algorithm. Uniform variation occurs when the variance of the optimal value of continuous convergence is within the allowable error. In this manner, the fish can escape the trap of the local extremum, obtaining the global optimal state. To validate the feasibility of improving AFSA, this paper conducts simulation experiments on portfolio problems using MATLAB tools. Experiments show that this model has an accuracy of 93.56 percent, which is 8.43 percent higher than that of the NSGA-II model and 3.76 percent higher than that of the multiobjective optimization model. The experiment shows that the algorithm in this paper can solve these types of problems well and that, using this model, the optimal portfolio investment decision scheme satisfying investors can be obtained. The optimized AFSA presented in this paper can serve as an important reference for investment portfolios and has a wide range of application possibilities in the investment market.
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http://dx.doi.org/10.1155/2022/7483454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9217557PMC
June 2022

Tumor-targeted/reduction-triggered composite multifunctional nanoparticles for breast cancer chemo-photothermal combinational therapy.

Acta Pharm Sin B 2022 Jun 10;12(6):2710-2730. Epub 2021 Sep 10.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center of Biotherapy, Chengdu 610041, China.

Breast cancer has become the most commonly diagnosed cancer type in the world. A combination of chemotherapy and photothermal therapy (PTT) has emerged as a promising strategy for breast cancer therapy. However, the intricacy of precise delivery and the ability to initiate drug release in specific tumor sites remains a challenging puzzle. Therefore, to ensure that the therapeutic agents are synchronously delivered to the tumor site for their synergistic effect, a multifunctional nanoparticle system (PCRHNs) is developed, which is grafted onto the prussian blue nanoparticles (PB NPs) by reduction-responsive camptothecin (CPT) prodrug copolymer, and then modified with tumor-targeting peptide cyclo(Asp-d-Phe-Lys-Arg-Gly) (cRGD) and hyaluronic acid (HA). PCRHNs exhibited nano-sized structure with good monodispersity, high load efficiency of CPT, triggered CPT release in response to reduction environment, and excellent photothermal conversion under laser irradiation. Furthermore, PCRHNs can act as a photoacoustic imaging contrast agent-guided PTT. studies indicate that PCRHNs exhibited excellent biocompatibility, prolonged blood circulation, enhanced tumor accumulation, allow tumor-specific chemo-photothermal therapy to achieve synergistic antitumor effects with reduced systemic toxicity. Moreover, hyperthermia-induced upregulation of heat shock protein 70 in the tumor cells could be inhibited by CPT. Collectively, PCRHNs may be a promising therapeutic way for breast cancer therapy.
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http://dx.doi.org/10.1016/j.apsb.2021.08.021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214336PMC
June 2022

Canonical Wnt signaling works downstream of iron overload to prevent ferroptosis from damaging osteoblast differentiation.

Free Radic Biol Med 2022 Jun 22. Epub 2022 Jun 22.

Laboratory of Skeletal Development and Regeneration, Institute of Life Sciences, Chongqing Medical University, Chongqing, 400016, China; Department of Orthopedics, Yongchuan Hospital, Chongqing Medical University, Chongqing, 402160, China; Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, 46202, USA. Electronic address:

Excessive iron has emerged in a large population of patients suffering from degenerative or hematological diseases with a common outcome, osteoporosis. However, its underlying mechanism remains to be clarified in order to formulate effective prevention and intervention against the loss of bone-forming osteoblasts. We show herein that increased intracellular iron by ferric ammonium citrate (FAC) mimicking the so-called non-transferrin bound iron concentrations leads to ferroptosis and impaired osteoblast differentiation. FAC upregulates the expression of Trfr and DMT1 genes to increase iron uptake, accumulating intracellular labile ferrous iron for iron overload status. Then, the excessive ferrous iron generates reactive oxygen species (ROS) and lipid peroxidation products (LPO), causing ferroptosis with its typical mitochondrial morphological changes, such as shrinkaged and condensed membrane with diminution and loss of crista and outer membrane rupture. We further examined that ferroptosis is the main cause responsible for FAC-disrupted osteoblast differentiation, although apoptosis and senescence are concurrently induced as well. Mechanistically, we revealed that iron dose-dependently down-regulates the expression of Wnt target genes and inhibits the transcription of Wnt reporter TopFlash construct, so as to inhibit the canonical Wnt signaling. Wnt agonist, ferroptosis inhibitor, or antioxidant melatonin reverses iron-inhibited canonical Wnt signaling to restore osteoblast differentiation by reducing ROS and LPO production to prevent ferroptosis notably without reducing iron overload. This study proposes a working model against excessive iron-induced osteoporosis: iron chelator deferoxamine or the above three drugs prevent ferroptosis, restore traditional Wnt signaling to maintain osteoblast differentiation no matter whether iron overload is removed or not. Additionally, iron chelator should be used to a suitable extent because iron itself is necessary for osteogenic differentiation.
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http://dx.doi.org/10.1016/j.freeradbiomed.2022.06.236DOI Listing
June 2022

Analysis and prediction models for operating speed of vehicles in expressway superlong tunnels based on geometric and traffic related parameters.

Traffic Inj Prev 2022 Jun 24:1-6. Epub 2022 Jun 24.

Chongqing CISDI Engineering Consulting Co., Ltd, Chongqing, China.

Objectives: Operating speeds on roads are critical indicators for evaluating traffic safety. Currently available research on the operating speed's prediction focuses on open roads and highways. Insufficient attention has been paid, so far, to tunnels, which form bottlenecks on expressways. The present research aims to establish an operating speed prediction model for tunnels and analyze the influence of their geometric parameters on the operating speeds of vehicles.

Methods: We consider the speed of vehicles collected through field measurements in the portals and lay-bys of six superlong tunnels (length greater than 3000 ). Using linear regression, a prediction model for the speed in an expressway superlong tunnel is obtained considering tunnel's geometric parameters. The influence of various parameters on the operating speed are analyzed through comparisons with existing research findings.

Results: We establish the first operating speed prediction model for tunnels considering geometric parameters and find that the vehicle type is the most important parameter affecting the operating speed. Other important parameters include the preceding curve length up to speed observation point (), preceding tangent length () and preceding tangent length up to speed observation point ().

Conclusions: The influence of geometric parameters on vehicle operating speed in super long tunnels differs from that observed in non-tunnel roadways. The effects of the preceding or subsequent curve radius ( or ) of the tangent section, curvature (), and curve degree () are not important in this case. Furthermore, we find that the influence of the posted speed limit () is closely related to the driving scene and safety awareness of drivers. These findings can improve the design and joint evaluation of tunnel geometric parameters and traffic safety.
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http://dx.doi.org/10.1080/15389588.2022.2087874DOI Listing
June 2022

Formulating High-Rate and Long-Cycle Heterostructured Layered Oxide Cathodes by Local Chemistry and Orbital Hybridization Modulation for Sodium-ion Batteries.

Adv Mater 2022 Jun 23:e2202695. Epub 2022 Jun 23.

Institute for Carbon Neutralization, College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou, Zhejiang, 325035, China.

It is still very urgent and challenging to simultaneously develop high-rate and long-cycle oxide cathodes for sodium-ion batteries (SIBs) because of its sluggish kinetics and complex multiphase evolution during cycling. Here, we report the concept of accurately manipulating structural evolution and formulating high-performance heterostructured biphasic layered oxide cathodes by local chemistry and orbital hybridization modulation. The P2-structure stoichiometric composition of cathode material shows a layered P2- and O3-type heterostructure that is explicitly evidenced by various macroscale and atomic-scale techniques. Surprisingly, the heterostructured cathode displays excellent rate performance, remarkable cycling stability (capacity retention of 82.16% after 600 cycles at 2C), and outstanding compatibility with hard carbon anode because of the integrated advantages of intergrowth structure and local environment regulation. Meanwhile, the formation process from precursors during calcination and the highly reversible dynamic structural evolution during the Na intercalation/deintercalation process are clearly articulated by a series of in situ characterization techniques. Also, the intrinsic structural properties and corresponding electrochemical behavior are further elucidating by the density of states and electron localization function of density functional theory calculations. Overall, this strategy, which finely tunes the local chemistry and orbitals hybridization for high-performance SIBs, will open up a new field for other materials. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202202695DOI Listing
June 2022

Ameliorative Effects of Gut Microbial Metabolite Urolithin A on Pancreatic Diseases.

Nutrients 2022 Jun 20;14(12). Epub 2022 Jun 20.

College of Food Science and Engineering, Northwest A&F University, Xianyang 712100, China.

Urolithin A (Uro A) is a dietary metabolite of the intestinal microbiota following the ingestion of plant-based food ingredients ellagitannins and ellagic acid in mammals. Accumulating studies have reported its multiple potential health benefits in a broad range of diseases, including cardiovascular disease, cancer, cognitive impairment, and diabetes. In particular, Uro A is safe via direct oral administration and is non-genotoxic. The pancreas plays a central role in regulating energy consumption and metabolism by secreting digestive enzymes and hormones. Numerous pathophysiological factors, such as inflammation, deficits of mitophagy, and endoplasmic reticulum stress, can negatively affect the pancreas, leading to pancreatic diseases, including pancreatitis, pancreatic cancer, and diabetes mellitus. Recent studies showed that Uro A activates autophagy and inhibits endoplasmic reticulum stress in the pancreas, thus decreasing oxidative stress, inflammation, and apoptosis. In this review, we summarize the knowledge of Uro A metabolism and biological activity in the gut, as well as the pathological features and mechanisms of common pancreatic diseases. Importantly, we focus on the potential activities of Uro A and the underlying mechanisms in ameliorating various pancreatic diseases via inhibiting inflammatory signaling pathways, activating autophagy, maintaining the mitochondrial function, and improving the immune microenvironment. It might present a novel nutritional strategy for the intervention and prevention of pancreatic diseases.
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http://dx.doi.org/10.3390/nu14122549DOI Listing
June 2022

Towards Understanding PRPS1 as a Molecular Player in Immune Response in Yellow Drum ().

Int J Mol Sci 2022 Jun 9;23(12). Epub 2022 Jun 9.

Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen 361021, China.

Phosphoribosyl pyrophosphate synthetases (EC 2.7.6.1) are key enzymes in the biological synthesis of phosphoribosyl pyrophosphate and are involved in diverse developmental processes. In our previous study, the gene was discovered as a key disease-resistance candidate gene in yellow drum, , in response to the infection of , through genome-wide association analysis. This study mainly focused on the characteristics and its roles in immune responses of the gene in yellow drum. In the present study, the gene was cloned from yellow drum, encoding a protein of 320 amino acids. Bioinformatic analysis showed that PRPS1 was highly conserved during evolution. Quantitative RT-PCR demonstrated that was highly expressed in the head-kidney and brain, and its transcription and translation were significantly activated by infection examined by RT-qPCR and immunohistochemistry analysis, respectively. Subcellular localization revealed that PRPS1 was localized in cytoplasm. In addition, semi-in vivo pull-down assay coupled with mass spectrometry identified myeloid differentiation factor 88 (MyD88) as an PRPS1-interacting patterner, and their interaction was further supported by reciprocal pull-down assay and co-immunoprecipitation. The inducible expression of by suggested that the linker molecule MyD88 in innate immune response may play together with PRPS1 to coordinate the immune signaling in yellow drum in response to the pathogenic infection. We provide new insights into important functions of PRPS1, especially PRPS1 in the innate immunity of teleost fishes, which will benefit the development of marine fish aquaculture.
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http://dx.doi.org/10.3390/ijms23126475DOI Listing
June 2022

Identification of large offspring syndrome during pregnancy through ultrasonography and maternal blood transcriptome analyses.

Sci Rep 2022 Jun 22;12(1):10540. Epub 2022 Jun 22.

Division of Animal Sciences, University of Missouri, 164 ASRC, 920 East Campus Drive, Columbia, MO, 65211, USA.

In vitro production (IVP) of embryos in cattle can result in large/abnormal offspring syndrome (LOS/AOS) which is characterized by macrosomia. LOS can cause dystocia and lead to the death of dam and calf. Currently, no test exists to identify LOS pregnancies. We hypothesized that fetal ultrasonography and/or maternal blood markers are useful to identify LOS. Bovine fetuses were generated by artificial insemination (control) or IVP. Fetal ultrasonographies were taken on gestation D55 (D55) and fetal collections performed on D56 or D105 (gestation in cattle ≈ D280). IVP fetuses weighing ≥ 97 percentile of the control weight were considered LOS. Ultrasonography results show that the product of six D55 measurements can be used to identify extreme cases of LOS. To determine whether maternal blood can be used to identify LOS, leukocyte mRNA from 23 females was sequenced. Unsupervised hierarchical clustering grouped the transcriptomes of the two females carrying the two largest LOS fetuses. Comparison of the leukocyte transcriptomes of these two females to the transcriptome of all other females identified several misregulated transcripts on gestation D55 and D105 with LOC783838 and PCDH1 being misregulated at both time-points. Together our data suggest that LOS is identifiable during pregnancy in cattle.
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http://dx.doi.org/10.1038/s41598-022-14597-wDOI Listing
June 2022

Endosymbiont population genomics sheds light on transmission mode, partner specificity, and stability of the scaly-foot snail holobiont.

ISME J 2022 Jun 17. Epub 2022 Jun 17.

Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, China.

The scaly-foot snail (Chrysomallon squamiferum) inhabiting deep-sea hydrothermal vents in the Indian Ocean relies on its sulphur-oxidising gammaproteobacterial endosymbionts for nutrition and energy. In this study, we investigate the specificity, transmission mode, and stability of multiple scaly-foot snail populations dwelling in five vent fields with considerably disparate geological, physical and chemical environmental conditions. Results of population genomics analyses reveal an incongruent phylogeny between the endosymbiont and mitochondrial genomes of the scaly-foot snails in the five vent fields sampled, indicating that the hosts obtain endosymbionts via horizontal transmission in each generation. However, the genetic homogeneity of many symbiont populations implies that vertical transmission cannot be ruled out either. Fluorescence in situ hybridisation of ovarian tissue yields symbiont signals around the oocytes, suggesting that vertical transmission co-occurs with horizontal transmission. Results of in situ environmental measurements and gene expression analyses from in situ fixed samples show that the snail host buffers the differences in environmental conditions to provide the endosymbionts with a stable intracellular micro-environment, where the symbionts serve key metabolic functions and benefit from the host's cushion. The mixed transmission mode, symbiont specificity at the species level, and stable intracellular environment provided by the host support the evolutionary, ecological, and physiological success of scaly-foot snail holobionts in different vents with unique environmental parameters.
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http://dx.doi.org/10.1038/s41396-022-01261-4DOI Listing
June 2022

Elevating the Photothermal Conversion Efficiency of Phase-Change Materials Simultaneously toward Solar Energy Storage, Self-Healing, and Recyclability.

ACS Appl Mater Interfaces 2022 Jun 17;14(25):29213-29222. Epub 2022 Jun 17.

State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu 610065, China.

To alleviate the predicament of resource shortage and environmental pollution, efficiently using abundant solar energy is a great challenge. Herein, we prepared unique photothermal conversion phase-change materials, namely, [email protected], by introducing carbon nanotubes (CNTs) used as photothermal conversion materials into the recyclable matrix of phase-change materials (PCMs). These devised [email protected] cleverly combine the photothermal conversion capability of CNTs and the thermal energy storage capability of traditional PCMs. Especially, the surface temperature of the prepared [email protected] can be raised to 100 °C within 165 s under the solar simulator (150 mW cm), showing a surprising heating rate that is much higher than that of the reported works and achieving a higher photothermal conversion efficiency for solar energy in this work. Furthermore, these [email protected] can hold high melting latent heat with a maximum value at 110.0 J g, exhibiting remarkable thermal storage ability aside from preeminent photothermal conversion capability. Intriguingly, the introduction of dynamic oxime group-carbamate bonds into the molecular structure can endow [email protected] with an outstanding self-healing performance and recyclability. The broken [email protected] sample can be healed in 2 min under IR-laser irradiation. Importantly, the phase-change and mechanical properties and photothermal conversion efficiency of [email protected] can also remain virtually unchanged after multiple recycles. It is of great significance to design this style of [email protected] for achieving the efficient utilization of solar energy and environmental protection.
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http://dx.doi.org/10.1021/acsami.2c05302DOI Listing
June 2022

Expanding the ReS Interlayer Promises High-Performance Potassium-Ion Storage.

ACS Appl Mater Interfaces 2022 Jun 17;14(25):28873-28881. Epub 2022 Jun 17.

Institute for Carbon Neutralization, College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou, Zhejiang 325035, China.

Improving the electrochemical kinetics and the intrinsic poor conductivity of transition metal dichalcogenide (TMD) electrodes is meaningful for developing next-generation energy storage systems. As one of the most promising TMD anode materials, ReS shows attractive performance in potassium-ion batteries (PIBs). To overcome the poor kinetic ion diffusion and limited cycling stability of the ReS-based electrode, herein, the interlayer distance expanding strategy was employed, and reduced graphene oxide (rGO) was introduced into ReS. Few-layered ReS nanosheets were grown on the surface of the rGO with expanded interlayer distance. The prepared ReS nanosheets show an expanded distance (∼0.77 nm). The synthesized [email protected] composites were used in PIBs as anode materials. The K-ion storage mechanism of the ReS-based anode was investigated by in situ X-ray diffraction (XRD) technology, which shows the intercalation and conversion types. The [email protected] nanocomposites show high specific capacities of 432.5, 316.5, and 241 mAh g under 0.05, 0.2, and 1.0 A g current densities and exhibit excellent reversibility at 1.0 A g. Overall, this strategy, which finely tunes the local chemistry and orbital hybridization for high-performance PIBs, will open up a new field for other materials.
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http://dx.doi.org/10.1021/acsami.2c05485DOI Listing
June 2022

Primary Graft Dysfunction: The Role of Aging in Lung Ischemia-Reperfusion Injury.

Front Immunol 2022 24;13:891564. Epub 2022 May 24.

Division of Transplant Surgery and Transplant Surgery Research Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, United States.

Transplant centers around the world have been using extended criteria donors to remedy the ongoing demand for lung transplantation. With a rapidly aging population, older donors are increasingly considered. Donor age, at the same time has been linked to higher rates of lung ischemia reperfusion injury (IRI). This process of acute, sterile inflammation occurring upon reperfusion is a key driver of primary graft dysfunction (PGD) leading to inferior short- and long-term survival. Understanding and improving the condition of older lungs is thus critical to optimize outcomes. Notably, lung perfusion (EVLP) seems to have the potential of reconditioning ischemic lungs through perfusing and ventilation. Here, we aim to delineate mechanisms driving lung IRI and review both experimental and clinical data on the effects of aging in augmenting the consequences of IRI and PGD in lung transplantation.
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http://dx.doi.org/10.3389/fimmu.2022.891564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170999PMC
June 2022

Three-pole wheel paddle luminescent metal organic frameworks (LMOFs) based on the oxygen substituted triazine tricarboxylic acid ligand: recognition and detection of small drug molecules and aromatic amine molecules.

Dalton Trans 2022 Jun 21;51(24):9336-9347. Epub 2022 Jun 21.

State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, P.R. China.

Luminescent metal organic frameworks (LMOFs) are considered to be a type of promising optical sensing material due to their designable and tunable functions, and stable pore structures. Therefore, the preparation of LMOFs has become a research hotspot in recent years. As we know, triazine carboxylic acid ligands are conducive for constructing LMOF materials due to their large π electron conjugated system. In this work, two crystalline materials [Cd(TCPT)]·0.5DMF·4HO (1) and (HO)[Zn(TCPT)(μ-OH)]·0.5DMF·3HO (2) were obtained by the reaction of the triazine carboxylic acid ligand 2,4,6-tris(4-carboxyphenoxy)-1,3,5-triazine (HTCPT), as an extended carboxylate arm, and d transition metal salts. Their structures were determined by single crystal X-ray diffraction and characterized by infrared spectroscopy (IR), ultraviolet visible spectroscopy (UV-vis), fluorescence spectroscopy, powder X-ray diffraction (PXRD) and thermogravimetric analysis (TG). The experimental results showed that complexes 1 and 2 show excellent fluorescent emission behavior. Thus, we explored their fluorescence sensing properties. To our delight, the results showed that they both had the ability to sense small organic drug molecules and aromatic amine molecules containing -phenylenediamine (OPD), -phenylenediamine (MPD) and -phenylenediamine (PPD). In general, the practical applications of a MOF material are usually limited because of the relatively harsh synthesis methods. In this aspect, we studied the synthesis method in detail to obtain the optimal reaction conditions for the large-scale synthesis of 1 and 2. The preparation of the two LMOF materials only required about 3 hours of heating time and they could be prepared on a large scale, which is significant for the practical applications of LMOFs.
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http://dx.doi.org/10.1039/d2dt01032aDOI Listing
June 2022

Incorporating Near-Pseudocapacitance Insertion Ni/Co-Based Hexacyanoferrate and Low-Cost Metallic Zn for Aqueous K-Ion Batteries.

ChemSusChem 2022 Jun 3:e202200706. Epub 2022 Jun 3.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, 430070, Wuhan, Hubei, P. R. China.

The limited availability of cathode materials with high specific capacity and significant cycling stability for aqueous K-ion batteries (AKIBs) hinder their further development owing to the large radius of K (1.38 Å). Prussian blue and its analogs with a three-dimensional frame structure possessing special energy storage mechanism are promising candidates as cathode materials for AKIBs. In this study, K Ni Co Fe(CN)  ⋅ 1.8H O (KNCHCF) was prepared as a cathode material for AKIBs. Both the electrochemical activity of Co ions and the near-pseudocapacitance intercalation of KNCHCF enhance K storage. Therefore, KNCHCF exhibits a superior capacity maintenance rate of 86 % after 1000 cycles at a high current density of 3.0 A g . The storage mechanism of K in AKIBs was revealed through ex situ X-ray diffraction, ex situ Fourier transform infrared spectroscopy, and ex situ X-ray photoelectron spectroscopy measurements. Moreover, the assembled K-Zn hybrid battery showed good cycling stability with 93.1 % capacity maintenance at 0.1 A g after 50 cycles and a high energy density of 96.81 W h kg . Hence, KNCHCF may be a potential material for the development of AKIBs.
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http://dx.doi.org/10.1002/cssc.202200706DOI Listing
June 2022

Fluorescence and absorbance dual-mode immunoassay for detecting Ochratoxin A.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Oct 30;279:121440. Epub 2022 May 30.

College of Life Science, Yangtze University, 266 Jingmi Road, Jingzhou, Hubei 434025, China; College of Animal Science, Yangtze University, 266 Jingmi Road, Jingzhou, Hubei 434025, China. Electronic address:

In this work, a simple dual-mode immunoassay for detecting Ochratoxin A (OTA) was developed by mixing G-quadruplex/N-methylmesoporphyrin IX (G4/NMM) and 3,3',5,5'-tetramethylbenzidine (TMB). The fluorescence of G4/NMM can be quenched by oxidized TMB (oxTMB) because the absorbance of oxTMB overlapped with the fluorescence emission of G4/NMM. In the absence of OTA, large amounts of oxTMB were formed with blue color and the fluorescence of G4/NMM was quenched. In the presence of OTA, the concentration of oxTMB was decreased, therefore the fluorescence of G4/NMM increased. The linear range of fluorescence immunoassay was 0.195-25 ng/mL, and the linear range of the absorbance immunoassay was 0.049-1.563 ng/mL. Thus, the linear range of this dual-mode immunoassay can be expanded to 0.049-25 ng/mL. Meanwhile, the new method showed good selectivity for OTA. Besides, the satisfactory recovery rates implied the new method had a potential value for practical sample detection.
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http://dx.doi.org/10.1016/j.saa.2022.121440DOI Listing
October 2022

Transcriptome and protein networks to elucidate the mechanism underlying nitrite degradation by Lactiplantibacillus plantarum.

Food Res Int 2022 Jun 10;156:111319. Epub 2022 May 10.

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Key Laboratory of Fruit & Vegetable Processing & Quality Control, Huazhong Agricultural University, Wuhan 430070, Hubei, China. Electronic address:

Excessive nitrite residue is one of the bottlenecks in the production of many fermented foods. Lactiplantibacillus plantarum PK25 obtained from traditional Chinese pickles exhibited excellent nitrite degradation ability. Here, transcriptome, protein-protein interaction networks, and phenotype were performed to evaluate systematically the mechanism of nitrite degradation of L. plantarum PK25. The results demonstrated that genes expression varied considerably at key time points for nitrite degradation. 553 (upregulated: 366, downregulated: 187) and 767 (upregulated: 425, downregulated: 342) differentially expressed genes were identified at 6 h and 24 h, respectively. The hub genes were mainly enriched in carbohydrate metabolism, energy metabolism, and nucleotide synthesis. PK25 expanded its carbon source utilizing profile and improved glycolysis to produce more ATP to counteract environmental stress. The related enzymes including glycoside hydrolase, sugar ABC transporter protein, and PTS sugar transporter were 5.714, 5.885, and 3.578-fold upregulated at the transcriptional level. For strain to sustain energy levels and acid generation, pyruvate metabolism was critical, with the result that phosphoenolpyruvate synthase and pyruvate oxidase were up-regulated to accelerate the pyruvate transition. To repair DNA lesions induced by nitrite, both base excision repair mechanism and recombinational DNA repair pathway were exploited, such as endodeoxyribonuclease upregulated 5.314 and 19.687-fold at the two moments. The results provided a theoretical reference and practical possibility to reduce nitrite residue and improve safety during food fermented products.
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http://dx.doi.org/10.1016/j.foodres.2022.111319DOI Listing
June 2022

Blocking phospholamban with VHH intrabodies enhances contractility and relaxation in heart failure.

Nat Commun 2022 May 31;13(1):3018. Epub 2022 May 31.

Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre (KI/AZ ICMC), Department of Medicine, Karolinska Institutet, Huddinge, Sweden.

The dysregulated physical interaction between two intracellular membrane proteins, the sarco/endoplasmic reticulum Ca ATPase and its reversible inhibitor phospholamban, induces heart failure by inhibiting calcium cycling. While phospholamban is a bona-fide therapeutic target, approaches to selectively inhibit this protein remain elusive. Here, we report the in vivo application of intracellular acting antibodies (intrabodies), derived from the variable domain of camelid heavy-chain antibodies, to modulate the function of phospholamban. Using a synthetic VHH phage-display library, we identify intrabodies with high affinity and specificity for different conformational states of phospholamban. Rapid phenotypic screening, via modified mRNA transfection of primary cells and tissue, efficiently identifies the intrabody with most desirable features. Adeno-associated virus mediated delivery of this intrabody results in improvement of cardiac performance in a murine heart failure model. Our strategy for generating intrabodies to investigate cardiac disease combined with modified mRNA and adeno-associated virus screening could reveal unique future therapeutic opportunities.
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http://dx.doi.org/10.1038/s41467-022-29703-9DOI Listing
May 2022

Icaritin-loaded PLGA nanoparticles activate immunogenic cell death and facilitate tumor recruitment in mice with gastric cancer.

Drug Deliv 2022 Dec;29(1):1712-1725

Department of Oncology, Innovation centre for Advanced Interdisciplinary Medicine, Guangzhou Key Laboratory of Enhanced Recovery after Abdominal Surgery, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, 510700, China.

This study aimed to explore the anti-tumor effect of icaritin loading poly (lactic-co-glycolic acid) nanoparticles (refer to [email protected] NPs) on gastric cancer (GC) cells. Transmission Electron Microscope (TEM), size distribution, zeta potential, drug-loading capability, and other physicochemical characteristics of [email protected] NPs were carried out. Furthermore, flow cytometry, confocal laser scanning microscope (CLSM), Cell Counting Kit-8 (CCK-8), Transwell, Elisa assay and Balb/c mice were applied to explore the cellular uptake, anti-proliferation, anti-metastasis, immune response activation effects, and related anti-tumor mechanism of [email protected] NPs and . [email protected] NPs showed spherical shape, with appropriate particle sizes and well drug loading and releasing capacities. Flow cytometry and CLSM results indicated that [email protected] could efficiently enter into GC cells. CCK-8 proved that [email protected] NPs dramatically suppressed cell growth, induced Lactic dehydrogenase (LDH) leakage, arrested more GC cells at G2 phase, and inhibited the invasion and metastasis of GC cells, compared to free icaritin. In addition, [email protected] could help generate dozens of reactive oxygen species (ROS) within GC cells, following by significant mitochondrial membrane potentials (MMPs) loss and excessive production of oxidative-mitochondrial DNA (Ox-mitoDNA). Since that, Ox-mitoDNA further activated the releasing of damage associated molecular pattern molecules (DAMPs), and finally led to immunogenic cell death (ICD). Our data also elaborated that [email protected] exerted a powerful inhibitory effect (∼80%), compared to free icaritin (∼60%). Most importantly, our results demonstrated that [email protected] could activate the anti-tumor immunity via recruitment of infiltrating CD4+ cells, CD8+ T cells and increased secretion of cytokine immune factors, including interferon-γ (IFN-γ) tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1). Our findings validate that the successful design of [email protected], which can effectively active ICD and facilitate tumor recruitment in GC through inducing mitoDNA oxidative damage.
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http://dx.doi.org/10.1080/10717544.2022.2079769DOI Listing
December 2022

sp. nov., an Attached Bacterium of .

Front Microbiol 2022 12;13:861291. Epub 2022 May 12.

College of Pharmacy and Life Science, Jiujiang University, Jiujiang, China.

In eutrophic water, attached bacteria of play an important role in the formation, development, and degradation of blooms. A novel actinobacterium, designated as JXJ CY 35, was isolated from the culture mass of FACHB-905 (Maf) collected from Lake Dianchi, Yunnan Province, China. Strain JXJ CY 35 was gram-positive, acid-fast staining, aerobic, with short rod-shaped cells, positive for catalase, and negative for oxidase. The isolate was able to grow at 10.0-36.0°C, pH 4.0-10.0, and tolerate up to 5.0% (w/v) NaCl, with optimal growth at 28°C, pH 7.0-8.0, and 0% (w/v) NaCl. Cell-wall peptidoglycan contains aspartic acid, glutamic acid, glycine, and alanine, with mannose, ribose, galactose, and arabinose as whole-cell sugars. Polar lipids consist of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), glycolipid (GL1-3), phosphoglycolipid (PGL), phosphatidylinositol (PI), and unidentified lipid (L1). The predominant menaquinone was MK-9. Major fatty acids (>10%) were Cω7c (37.0%) and Cω9c (18.9%). The complete genome sequence of strain JXJ CY 35 was 6,138,096 bp in size with a DNA G + C content of 68.3%. Based on 16S rRNA gene sequences, it has 98.2% similarity to JCM 18538. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain JXJ CY 35 and the closest five type strains JCM 18538, ATCC 700504, DSM 44476, DSM 44191, and CECT 8778 were 52.1, 20.3, 20.3, 20.6, and 19.8%, and 92.7, 75.5, 75.6, 76.0, and 75.2%, respectively. On the basis of the above taxonomic data and differences in physiological characteristics from the closely related type strain, strain JXJ CY 35 was determined to represent a novel species of genus , for which the name sp. nov., is proposed. The type strain is JXJ CY 35 (=KCTC 49379 = CGMCC 1.17501). Different inoculation dosages of the type strain JXJ CY 35 could exhibit different effects on the growth of Maf and its toxin synthesis and release. Strain JXJ CY 35 could promote the growth of Maf by providing it with available phosphorus, nitrogen, probably vitamins, and plant growth hormones.
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http://dx.doi.org/10.3389/fmicb.2022.861291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134240PMC
May 2022

Effective Skill Transfer From Fundamentals of Arthroscopic Surgery Training to Shoulder Arthroscopic Simulator in Novices.

Surg Innov 2022 May 24:15533506221104379. Epub 2022 May 24.

Clinical Skills Training Centre, Zhujiang Hospital, 70570Southern Medical University, Guangzhou, China.

Purpose: To investigate whether novices could improve performance on a shoulder arthroscopic simulator (high-fidelity) through short-term training on a Fundamentals of Arthroscopic Surgery Training (FAST) simulator (low-fidelity).

Methods: Twenty-eight novices with no experience in arthroscopy were recruited to perform a pre-test on a shoulder arthroscopic simulator. Then they were randomized into two groups: the experimental group practiced five modules on the FAST simulator three times, and the control group did nothing. The experimental group performed a post-test immediately after FAST simulator practice. Control group rested for 70 minutes after experiencing pre-test before performing post-test. All parameters were recorded by the simulator.

Results: The experimental group outperformed the control group in terms of total score, procedure time, camera path length, and grasper path length. However, there was no statistical difference in scratching of humerus cartilage or glenoid cartilage. Significant differences were found in the improvement of both groups in total score, procedure time, and camera path length.

Conclusions: Arthroscopic skills gained after short-term training on FAST simulator could be transferred to the shoulder arthroscopic simulator. This research provides important evidence of the benefits of FAST simulator in shoulder arthroscopy training program.
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http://dx.doi.org/10.1177/15533506221104379DOI Listing
May 2022

A new model based inflammatory index and tumor burden score (TBS) to predict the recurrence of hepatocellular carcinoma (HCC) after liver resection.

Sci Rep 2022 May 23;12(1):8670. Epub 2022 May 23.

Department of General Surgery, Xiangya Hospital, Central South University, Changsha, 410008, China.

To establish a model based on inflammation index and tumor burden score (TBS) to predict recurrence of hepatocellular carcinoma (HCC) after liver resection. A retrospective study was performed on 217 patients who diagnosed HCC underwent liver resection at Xiangya Hospital Central South University from June 1, 2017 to June 1, 2019. According to the receiver operating characteristic (ROC) curve, the optimal cut-off value of inflammatory index and the TBS was determined by the Youden index. Prediction performance was compared by the area under the receiver operating characteristic curve (AUC). Cox regression analysis was used to determine the risk factors for the recurrence of HCC after liver resection. According to the independent risk factors of the patients, a prediction model for HCC was established based on inflammation index and tumor burden score (TBS).The prediction performance of the model was compared with single index (TBS group and NLR group) and traditional HCC stage models (TNM stage and BCLC stage). MLR = 0.39, NLR = 2.63, PLR = 134, SII = 428 and TBS = 8.06 are the optimal cut-off values. AUC of SII, PLR, NLR, MLR and TBS were 0.643, 0.642, 0.642, 0.618 and 0.724respectively. MVI (P = 0.005), satellite nodule (P = 0.017), BCLC B-C stage (P = 0.013), NLR > 2.63 (P = 0.013), TBS > 8.06 (P = 0.017) are independent risk factors for the recurrence of HCC after liver resection. According to this study, the optimal inflammatory index NLR combined with TBS was obtained. The AUC of NLR-TBS model was 0.762, not only better than NLR group (AUC = 0.630) and TBS group (AUC = 0.671), also better than traditional BCLC (AUC = 0.620) and TNM (AUC = 0.587) stage models. Interestingly, we found that NLR and TBS should be good prognostic factor for recurrence of HCC after liver resection. The NLR-TBS model based the best inflammatory index (NLR) and TBS have a better prediction performance and the prediction performance of NLR-TBS model not only better than NLR group and TBS group, but better than BCLC and TNM stage models.
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http://dx.doi.org/10.1038/s41598-022-12518-5DOI Listing
May 2022

Mechanical loading attenuated negative effects of nucleotide analogue reverse-transcriptase inhibitor TDF on bone repair via Wnt/β-catenin pathway.

Bone 2022 Aug 21;161:116449. Epub 2022 May 21.

Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, China. Electronic address:

The nucleotide analog reverse-transcriptase inhibitor, tenofovir disoproxil fumarate (TDF), is widely used to treat hepatitis B virus (HBV) and human immunodeficiency virus infection (HIV). However, long-term TDF usage is associated with an increased incidence of bone loss, osteoporosis, fractures, and other adverse reactions. We investigated the effect of chronic TDF use on bone homeostasis and defect repair in mice. In vitro, TDF inhibited osteogenic differentiation and mineralization in MC3T3-E1 cells. In vivo, 8-week-old C57BL/6 female mice were treated with TDF for 38 days to simulate chronic medication. Four-point bending test and μCT showed reduced bone biomechanical properties and microarchitecture in long bones. To investigate the effects of TDF on bone defect repair, we utilized a bilateral tibial monocortical defect model. μCT showed that TDF reduced new bone mineral tissue and bone mineral density (BMD) in the defect. To verify whether mechanical stimulation may be a useful treatment to counteract the negative bone effects of TDF, controlled dynamic mechanical loading was applied to the whole tibia during the matrix deposition phase on post-surgery days (PSDs) 5 to 8. Second harmonic generation (SHG) of collagen fibers and μCT showed that the reduction of new bone volume and bone mineral density caused by TDF was reversed by mechanical loading in the defect. Immunofluorescent deep tissue imaging showed that chronic TDF treatment reduced the number of osteogenic cells and the volume of new vessels. In addition, chronic TDF treatment inhibited the expressions of periostin and β-catenin, but increased the expression of sclerostin. Both negative effects were reversed by mechanical loading. Our study provides strong evidence that chronic use of TDF exerts direct and inhibitory impacts on bone repair, but appropriate mechanical loading could reverse these adverse effects.
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http://dx.doi.org/10.1016/j.bone.2022.116449DOI Listing
August 2022

Tetraphenylethylene-Functionalized Metal-Organic Frameworks with Strong Aggregation-Induced Electrochemiluminescence for Ultrasensitive Analysis through a Multiple Convertible Resonance Energy Transfer System.

Anal Chem 2022 Jun 22;94(22):7861-7867. Epub 2022 May 22.

Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Collaborative Innovation Center for Advanced Organic Chemical Materials Co-constructed by the Province and Ministry, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.

Since aggregation-induced electrochemiluminescence (AIECL) combined the merits of aggregation-induced emission (AIE) and electrochemiluminescence (ECL), it has become a research hotspot recently. Herein, novel kinds of functional metal-organic frameworks (MOFs) with strong AIECL were reported through doping tetraphenylethylene (TPE) into UiO-66. Due to the porosity and highly ordered topological structure that caused the confinement effect of MOFs, the molecular motion of TPE was effectively limited within UiO-66, resulting in strong AIE. Meanwhile, the large specific surface area and porous structure of UiO-66 allowed TPE to react with coreactants more effectively, which was beneficial to ECL. Thus, the TPE-functionalized UiO-66 (TPE-UiO-66) showed excellent AIECL performance surprisingly. Inspired by this, a multiple convertible ECL resonance energy transfer (ECL-RET) system was constructed through a DNA Y structure that regulated the distance between the energy donor (TPE-UiO-66) and different energy acceptors (gold nanoparticles and Adriamycin). Furthermore, an ultrasensitive ECL biosensor for the detection of Mucin 1 (MUC1) was developed through the introduction of the novel ECL-RET system. In the presence of MUC1, the DNA Y structure was constructed, keeping the gold nanoparticles (AuNPs) away from TPE-UiO-66. Then, Adriamycin (Dox) could be embedded in the DNA Y structure and act as an energy acceptor to receive the energy of TPE-UiO-66, which made the biosensor produce a strong ECL response. As expected, the developed ECL biosensor exhibited superior detection performance for MUC1. This work provided a novel way to realize AIECL and board the application of AIECL in analytical chemistry.
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http://dx.doi.org/10.1021/acs.analchem.2c00295DOI Listing
June 2022

General synthesis of 2D rare-earth oxide single crystals with tailorable facets.

Natl Sci Rev 2022 May 23;9(5):nwab153. Epub 2021 Aug 23.

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.

Two-dimensional (2D) rare-earth oxides (REOs) are a large family of materials with various intriguing applications and precise facet control is essential for investigating new properties in the 2D limit. However, a bottleneck remains with regard to obtaining their 2D single crystals with specific facets because of the intrinsic non-layered structure and disparate thermodynamic stability of different facets. Herein, for the first time, we achieve the synthesis of a wide variety of high-quality 2D REO single crystals with tailorable facets via designing a hard-soft-acid-base couple for controlling the 2D nucleation of the predetermined facets and adjusting the growth mode and direction of crystals. Also, the facet-related magnetic properties of 2D REO single crystals were revealed. Our approach provides a foundation for further exploring other facet-dependent properties and various applications of 2D REO, as well as inspiration for the precise growth of other non-layered 2D materials.
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http://dx.doi.org/10.1093/nsr/nwab153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113103PMC
May 2022

Dietary glycemic index, glycemic load intake, and risk of lung cancer: A meta-analysis of observational studies.

Nutrition 2022 Jul-Aug;99-100:111676. Epub 2022 Apr 1.

Wuhan Wuchang Hospital, Wuchang Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, Hubei Province, China. Electronic address:

This meta-analysis evaluated the relationship between dietary glycemic index (GI), glycemic load (GL), and lung cancer risk, which has been controversial in previous studies. We searched PubMed, Embase, Web of Science, and Cochrane Library databases for relevant studies from inception to October 2021 in English only. We included case-control and cohort studies that reported relative risks (RRs) and 95% confidence intervals (CI) between dietary GI or GL intake and lung cancer risk. Either a fixed- or random-effects model was used to estimate pooled RRs. Subgroup analysis, sensitivity analysis, meta-regression, publication bias test, and dose-response meta-analysis were performed. We identified nine studies eligible for meta-analysis with 32810 cases and 351013 controls. The pooled RR for highest versus lowest intake was 1.14 (95% CI: 1.03-1.26, I=64.8%, P=0.002) for GI and 0.93 (95% CI: 0.84-1.02, I=42.3%, P=0.076) for GL. Subgroup analysis showed that the associations between GI or GL and lung cancer were similar between groups. Sensitivity analysis revealed reduced heterogeneity among GL-related studies when one particular study was excluded. There was no evidence of publication bias. A linear association between GI intake and lung cancer risk was observed. The present meta-analysis suggests that high dietary GI intake is associated with a significantly increased risk of lung cancer in a linear fashion. However, no significant association was observed between GL and lung cancer risk.
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http://dx.doi.org/10.1016/j.nut.2022.111676DOI Listing
June 2022

Which Is the Optimum Antigen Concentration for the Venereal Disease Research Laboratory Test of Cerebrospinal Fluid for Neurosyphilis Diagnosis: 10 or 17 μL?

Front Med (Lausanne) 2022 28;9:877186. Epub 2022 Apr 28.

Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, China.

The manufacturer's instructions for the venereal disease research laboratory (VDRL) antigen test for diagnosing neurosyphilis describe testing of serum samples and do not include procedures for cerebrospinal fluid (CSF) testing. This study compared the CSF-VDRL test with 10 μL of antigen (CSF-VDRL-10) according to the American Public Health Association to the CSF-VDRL test with 17 μL of antigen (CSF-VDRL-17) according to the VDRL serum procedure. A total of 121 neurosyphilis patients and 86 syphilis/non-neurosyphilis patients were included. The sensitivities of the CSF-VDRL-10 and CSF-VDRL-17 tests were comparable for neurosyphilis diagnosis. The positive rate of the CSF-VDRL-17 test was higher than that of the CSF-VDRL-10 test. In all, 78.3% of the quantitative CSF-VDRL-17 results were consistent with those of the CSF-VDRL-10 test, 18.4% exhibited one-titer higher results than those of the CSF-VDRL-10 test, and 3.4% had positive CSF-VDRL-17 results but negative CSF-VDRL-10 results. The CSF-VDRL test with 17 μL of antigen was more sensitive, and it is worth performing longitudinal studies to understand its practical implications.
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http://dx.doi.org/10.3389/fmed.2022.877186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095981PMC
April 2022

Forecasting the transmission trends of respiratory infectious diseases with an exposure-risk-based model at the microscopic level.

Environ Res 2022 Sep 12;212(Pt C):113428. Epub 2022 May 12.

Guangdong Provincial Engineering Technology Research Center of Environmental and Health Risk Assessment, Department of Occupational and Environmental Health, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong, China. Electronic address:

Respiratory infectious diseases (e.g., COVID-19) have brought huge damages to human society, and the accurate prediction of their transmission trends is essential for both the health system and policymakers. Most related studies focus on epidemic trend forecasting at the macroscopic level, which ignores the microscopic social interactions among individuals. Meanwhile, current microscopic models are still not able to sufficiently decipher the individual-based spreading process and lack valid quantitative tests. To tackle these problems, we propose an exposure-risk-based model at the microscopic level, including 4 modules: individual movement, virion-laden droplet movement, individual exposure risk estimation, and prediction of transmission trends. Firstly, the front two modules reproduce the movements of individuals and the droplets of infectors' expiratory activities, respectively. Then, the outputs are fed to the third module to estimate the personal exposure risk. Finally, the number of new cases is predicted in the final module. By predicting the new COVID- 19 cases in the United States, the performances of our model and 4 other existing macroscopic or microscopic models are compared. Specifically, the mean absolute error, root mean square error, and mean absolute percentage error provided by the proposed model are respectively 2454.70, 3170.51, and 3.38% smaller than the minimum results of comparison models. The quantitative results reveal that our model can accurately predict the transmission trends from a microscopic perspective, and it can benefit the further investigation of many microscopic disease transmission factors (e.g., non-walkable areas and facility layouts).
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http://dx.doi.org/10.1016/j.envres.2022.113428DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095069PMC
September 2022

A Study of Carbon Emission Efficiency in Chinese Provinces Based on a Three-Stage SBM-Undesirable Model and an LSTM Model.

Int J Environ Res Public Health 2022 04 28;19(9). Epub 2022 Apr 28.

Liaoning Banking and Insurance Regulatory Bureau, Shenyang 110013, China.

As a major carbon-emitting country, there is an urgent need for China to reduce carbon emissions. Studying the carbon emission efficiency of each province helps us to learn about the characteristics and evolution of regional carbon emissions, which is important for proposing effective and targeted measures to achieve the carbon peaking and carbon neutrality goals. This paper measures the carbon emission efficiency of 30 Chinese provinces from 2006 to 2019 based on a three-stage SBM-undesirable model and explores external drivers using stochastic frontier models. The results of the SBM-undesirable model show that the inter-provincial carbon emission efficiency is unevenly distributed and shows a big difference. From the results of the stochastic frontier model analysis, external drivers such as the intensity of finance in environmental protection, the level of economic development, the industrial structure, the level of urbanization, the degree of openness and the level of science as well as technology innovation all have an impact on the emission efficiency. In terms of LSTM model prediction, the model shows an excellent fitting effect, which provides a possible path for carbon emission efficiency prediction. Finally, based on the empirical results and the actual situation of each province in China, this paper proposes relevant feasible suggestions.
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http://dx.doi.org/10.3390/ijerph19095395DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101396PMC
April 2022

Predicting Novel Drug Candidates for Pancreatic Neuroendocrine Tumors via Gene Signature Comparison and Connectivity Mapping.

J Gastrointest Surg 2022 May 4. Epub 2022 May 4.

Department of Surgery, The Ohio State University Wexner Medical Center and James Cancer Hospital and Solove Research Institute, 395 W. 12th Ave., Suite 670, Columbus, OH, USA.

Introduction: There is a paucity of effective treatment options for advanced pancreatic neuroendocrine tumors (pNETs). Genome-wide analyses may allow for potential drugs to be identified based on differentially expressed genes (DEGs).

Methods: Oligo microarray data of RNA expression profiling of pNETs and normal pancreas tissues were downloaded from the Gene Expression Omnibus. Functional and pathway enrichment information of the DEGs was obtained using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases. Corresponding homologous proteins were analyzed and potential therapeutic drugs for pNETs were identified using the Connectivity Map and Drug-Gene Interaction Database.

Results: Assessment of raw data from 12,610 pNET genes demonstrated that 1082 and 380 genes were upregulated and downregulated, respectively, compared with normal pancreas tissue. Upregulated pathways were associated with nitrogen metabolism (i.e., GABAergic synapse, and graft-versus-host disease), whereas downregulated pathways included C-type leptin receptor signaling pathway, pertussis and AMPK signaling pathway. In particular, the protein-protein interaction analysis revealed 10 upregulated hub genes (DYNLL1, GNB5, GNB2, GNG4, GNAI2, GNAI1, HIST2H2BE, NUP107, NUP133, and SNAP25) and 10 downregulated hub genes (CXCL8, F2, CXCL2, GCG, SST, INS, GALR3, CCL20, ADRA2B, and CXCL6). Using the Drug-Gene Interaction Database, candidate drugs for pNETs treatment included 3 EGFR inhibitors (canertinib, erlotinib, WZ-4-145), as well as other cell-signaling pathway inhibitors such as AG-592, acarbose, lonidamine, azacytidine, rottlerin, and HU-211.

Conclusion: Using available genetic atlas data, potential drug candidates for treatment of pNETs were identified based on differentially expressed genes. These results may help focus efforts on identifying targeted agents with therapeutic efficacy to treat patients with pNETs.
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http://dx.doi.org/10.1007/s11605-022-05337-6DOI Listing
May 2022
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