Publications by authors named "Yao Wang"

2,144 Publications

  • Page 1 of 1

Mutant B2M-HLA-E and B2M-HLA-G fusion proteins protects universal chimeric antigen receptor-modified T cells from allogeneic NK cell-mediated lysis.

Eur J Immunol 2021 Jul 29. Epub 2021 Jul 29.

Department of Bio-therapeutic, the First Medical Centre, Chinese PLA General Hospital, Beijing, China.

Recent studies have indicated the anti-tumor activity and reduced allogeneic response of universal chimeric antigen receptor-modified T (UCAR T) cells lacking endogenous T cell receptors and beta-2 microglobulin (B2M) generated using gene-editing technologies. However, these cells are vulnerable to lysis by allogeneic natural killer (NK) cells due to their lack of human leukocyte antigen (HLA) class I molecule expression. Here, constitutive expression of mutant B2M-HLA-E (mBE) and B2M-HLA-G (mBG) fusion proteins in anti-CD19 UCAR T (UCAR T-19) cells was conducted to protect against allogeneic NK cell-mediated lysis. The ability of cells expressing mBE or mBG to resist NK cell-mediated lysis was observed in gene-edited Jurkat CAR19 cells. UCAR T-19 cells constitutively expressing the mBE and mBG fusion proteins were manufactured and showed effective and specific anti-tumor activity. Constitutive expression of the mBE and mBG fusion proteins in UCAR T-19 cells prevented allogeneic NK cell-mediated lysis. In addition, these cells were not recognizable by allogeneic T cells. Additional experiments, including those in animal models and clinical trials, are required to evaluate the safety and efficacy of UCAR T-19 cells that constitutively express mBE and mBG. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/eji.202049107DOI Listing
July 2021

Estimating the transmissibility of hepatitis C: a modelling study in Yichang City, China.

J Viral Hepat 2021 Jul 27. Epub 2021 Jul 27.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen City, Fujian Province, People's Republic of China.

Yichang is a city in central China in the Hubei Province. This study aimed to estimate the dynamics of the transmissibility of hepatitis C using a mathematical model and predict the transmissibility of hepatitis C in 2030. Data of hepatitis C cases from 13 counties or districts (cities) in Yichang from 2008 to 2016 were collected. A Susceptible-Infectious-Chronic-Recovered (SICR) model was developed to fit the data. The transmissibility of hepatitis C at the counties or districts was calculated based on new infections (including infected or chronically infected cases) reported monthly in the city caused by one infectious individual (MNI). The trend of the MNI was fitted and predicted using 11 models, with the coefficient of determination (R ) was being used to test the goodness of fit of these models. A total of 3065 cases of hepatitis C were reported in Yichang from 2008 to 2016. The median MNI of Yichang was 0.0768. According to the fitting results and analysis, the trend of transmissibility of hepatitis C in Yichang City conforms with the Logarithmic (R = 0.918, P < 0.001):MNI = 0.265 - 0.108log(t), and Exponential (R = 0.939, P < 0.001):MNI = 0.344 models. Hence, the transmission of hepatitis C virus at the county level has a downward trend. In conclusion, the transmissibility of hepatitis C in Yichang has a downward trend. With the current preventive and control measures in place, the spread of hepatitis C can be controlled.
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http://dx.doi.org/10.1111/jvh.13582DOI Listing
July 2021

Exosomes derived from astrocytes after oxygen-glucose deprivation promote differentiation and migration of oligodendrocyte precursor cells in vitro.

Mol Biol Rep 2021 Jul 26. Epub 2021 Jul 26.

Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1095 Jiefang Avenue, Wuhan, 430030, People's Republic of China.

Background: Excessive release of glutamate, oxidative stress, inflammation after ischemic brain injury can lead to demyelination. Astrocytes participate in the maturation and differentiation of oligodendrocyte precursor cells (OPCs), and play multiple roles in the process of demyelination and remyelination. Here, we studied the role of Astrocyte-derived exosomes (AS-Exo) under ischemic conditions in proliferation, differentiation and migration of OPCs in vitro.

Methods And Results: Exosomes were collected from astrocytes supernatant by differential centrifugation from control astrocytes (CT), mild hypoxia astrocytes (O2R24) which were applied oxygen-glucose deprivation for 2 h and reperfusion for 24 h (OGD2hR24h) and severe hypoxia astrocytes (O4R24) which were applied oxygen-glucose deprivation for 4 h and reperfusion for 24 h (OGD4hR24h). Exosomes (20 µg/ml) were co-cultured with OPCs for 24 h and their proliferation, differentiation and migration were detected. The results showed that AS-Exo under severe hypoxia (O4R24) inhibit the proliferation of OPCs. Meanwhile, all exosomes from three groups can promote OPCs differentiation and migration. Compared to control, the expressions of MAG and MBP, markers of mature oligodendrocytes, were significantly increased in AS-Exo treatment groups. AS-Exo treatment significantly increased chemotaxis for OPCs.

Conclusions: AS-Exo improve OPCs' differentiation and migration, whereas AS-Exo with severe hypoxic precondition suppress OPCs' proliferation. AS-Exo may be a potential therapeutic target for myelin regeneration and repair in white matter injury or other demyelination related diseases.
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http://dx.doi.org/10.1007/s11033-021-06557-wDOI Listing
July 2021

Depression of Pyroptosis by Inhibiting Caspase-1 Activation Improves Neurological Outcomes of Kernicterus Model Rats.

ACS Chem Neurosci 2021 Jul 23. Epub 2021 Jul 23.

Department of Neonatology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing 400014, China.

Kernicterus is a severe complication of extreme neonatal hyperbilirubinemia. Prolonged exposure to high-level unconjugated bilirubin (UCB) directly damages brain tissue. Neuroinflammation is believed to contribute to UCB-induced neurotoxicity. Pyroptosis has been as a highly inflammatory form of programmed cell death. Therefore, this study aimed to explore whether pyroptosis was involved in the pathogenesis of UCB neurotoxicity in kernicterus model rats. VX-765, a specific inhibitor of caspase-1, was intraperitoneally administered to the model rats to observe its effects on the short-term and long-term outcomes of the model animals at the molecular, cellular, morphological, and behavioral levels. The results indicated that UCB significantly induced the activation of caspase-1 and gasdermin D(GSDMD), and VX-765 inhibited caspase-1-GSDMD pathway. Compared with those of the UCB group and the vehicle+UCB group, VX-765-treated rats released lower levels of IL-1β and IL-18. Furthermore, H&E and TUNEL staining showed that nerve cells in the VX-765-treated group were better preserved and had less DNA fragmentation. Most importantly, VX-765 improved both the short-term and long-term neurological functions of kernicterus model rats. This study demonstrated that pyroptosis was involved in the pathogenesis of kernicterus through caspase-1 activation, which could be inhibited by VX-765, exerting a neuroprotective effect in kernicterus model rats.
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http://dx.doi.org/10.1021/acschemneuro.1c00287DOI Listing
July 2021

The effects of platelet-rich plasma combined with a skin flap transplant on open foot fractures with soft tissue defects.

Am J Transl Res 2021 15;13(6):6662-6669. Epub 2021 Jun 15.

Department of Hand and Foot Microsurgery, Yuyao City People's Hospital Yuyao 315400, Zhejiang Province, China.

Objective: This study aimed to explore the therapeutic effect of surgery using platelet-rich plasma combined with a skin flap transplant on open foot fractures with a soft tissue defect.

Methods: From February 2017 to March 2020, 72 patients with open foot fractures and soft tissue defects were recruited as the study cohort. The patients who underwent surgery with just a flap transplant were placed in the control group (the CG) (35 cases), and the patients who underwent surgery using platelet-rich plasma combined with a flap transplant were placed in the research group (the RG) (37 cases). The wound volume changes before and after the treatment, the wound healing times, the fracture healing times, and any adverse prognostic reactions were observed. The pre- and post-treatment VAS and SF-36 scores were observed and recorded.

Results: The total effective rate in the RG (100.00%) was significantly higher than the total effective rate in the CG (88.57%) (P=0.034). After the treatment, the wound volumes were lower in both groups, and the volume in the RG was smaller than it was in the CG at 3 weeks and 6 weeks after the treatment (P < 0.05). The average wound healing time in the RG (22.40 ± 2.10 days) was significantly lower than it was in the CG (32.20 ± 3.30 days) (P > 0.05). The average fracture healing time in the RG (6.50 ± 2.20 months) was significantly lower than it was in the RG (7.51 ± 2.33 months) (P > 0.05). The total incidence of adverse reactions to the treatment in the RG was 2.70%, and in the CG it was 11.43%. After the treatment, the VAS scores in the RG were significantly lower than they were in the CG (P < 0.05), and the SF-36 scores in the RG were significantly higher than the SF-36 scores in the CG (P < 0.05).

Conclusion: Platelet-rich plasma combined with a skin flap transplant can accelerate the healing times of wounds and fractures and lessen the occurrence of adverse reactions during the patients' treatment.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290717PMC
June 2021

Continual Decline in Azole Susceptibility Rates in Over a 9-Year Period in China.

Front Microbiol 2021 9;12:702839. Epub 2021 Jul 9.

Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.

Background: There have been reports of increasing azole resistance in , especially in the Asia-Pacific region. Here we report on the epidemiology and antifungal susceptibility of causing invasive candidiasis in China, from a 9-year surveillance study.

Methods: From August 2009 to July 2018, isolates ( = 3702) were collected from 87 hospitals across China. Species identification was carried out by mass spectrometry or rDNA sequencing. Antifungal susceptibility was determined by Clinical and Laboratory Standards Institute disk diffusion (CHIF-NET10-14, = 1510) or Sensititre YeastOne (CHIF-NET15-18, = 2192) methods.

Results: Overall, 22.2% (823/3702) of the isolates were resistant to fluconazole, with 90.4% (744/823) being cross-resistant to voriconazole. In addition, 16.9 (370/2192) and 71.7% (1572/2192) of the isolates were of non-wild-type phenotype to itraconazole and posaconazole, respectively. Over the 9 years of surveillance, the fluconazole resistance rate continued to increase, rising from 5.7 (7/122) to 31.8% (236/741), while that for voriconazole was almost the same, rising from 5.7 (7/122) to 29.1% (216/741), with no significant statistical differences across the geographic regions. However, significant difference in fluconazole resistance rate was noted between isolates cultured from blood (27.2%, 489/1799) and those from non-blood (17.6%, 334/1903) specimens (-value < 0.05), and amongst isolates collected from medical wards (28.1%, 312/1110) versus intensive care units (19.6%, 214/1092) and surgical wards (17.9%, 194/1086) (Bonferroni adjusted -value < 0.05). Although echinocandin resistance remained low (0.8%, 18/2192) during the surveillance period, it was observed in most administrative regions, and one-third (6/18) of these isolates were simultaneously resistant to fluconazole.

Conclusion: The continual decrease in the rate of azole susceptibility among strains has become a nationwide challenge in China, and the emergence of multi-drug resistance could pose further threats. These phenomena call for effective efforts in future interventions.
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http://dx.doi.org/10.3389/fmicb.2021.702839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299486PMC
July 2021

Discovery of a novel plant-derived agent against Ralstonia solanacearum by targeting the bacterial division protein FtsZ.

Pestic Biochem Physiol 2021 Aug 5;177:104892. Epub 2021 Jun 5.

Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing 400715, China. Electronic address:

Ralstonia solanacearum (R. solanacearum) is one of the most devastating bacterial pathogens and leads to serious economic losses in crops worldwide. In this study, the antibacterial activities of novel plant-derived coumarins against R. solanacearum and their underlying mechanisms were initially investigated. The bioactivity assay results showed that certain coumarins had significant in vitro inhibitory effects against R. solanacearum. Notably, 6-methylcoumarin showed the best in vitro antibacterial activity with 76.79%. Interestingly, 6-methylcoumarin was found to cause cell elongation, disrupt cell division, and suppress the expression of the bacterial division protein coding genes ftsZ. Compared with the control treatment, the ∆ftsZ mutant inhibited bacterial growth and caused the bacteria to be more sensitive to 6-methylcoumarin. The application of 6-methylcoumarin effectively suppressed the development of tobacco bacterial wilt in pot and field experiments, and significantly reduced the bacterial population in tobacco stems. The control efficiency of 6-methylcoumarin treatment was 35.76%, 40.51%, 38.99% at 10, 11, and 12 weeks after tobacco transplantation in field condition. All of these results demonstrate that 6-methylcoumarin has potential as an eco-friendly and target specificity agent for controlling tobacco bacterial wilt.
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http://dx.doi.org/10.1016/j.pestbp.2021.104892DOI Listing
August 2021

Developing Two Rapid Protein Extraction Methods Using Focused-Ultrasonication and Zirconia-Silica Beads for Filamentous Fungi Identification by MALDI-TOF MS.

Front Cell Infect Microbiol 2021 6;11:687240. Epub 2021 Jul 6.

Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Filamentous fungi identification by Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been challenging due to the lack of simple and rapid protein extraction methods and insufficient species coverage in the database. In this study, we created two rapid protein extraction methods for filamentous fungi: a one-step zirconia-silica beads method (ZSB) and a focused-ultrasonication method (FUS). The identification accuracy of two methods were evaluated with the VITEK MS, as well as number of spectra peaks and signal-to-noise ratio (S/N) with M-Discover 100 MALDI-TOF MS compared to the routine method. The better method was applied to build a filamentous fungi in-house spectra library for the M-Discover 100 MS, and then another one and routine method were performed in parallel to verify the accuracy and commonality of the in-house library. Using the two optimized methods, the dedicated operating time before MALDI-TOF MS analysis was reduced from 30 min to 7 (ZSB) or 5 (FUS) min per sample, with only a few seconds added for each additional strain. And both two methods identified isolates from most mold types equal to or better than the routine method, and the total correct identification rate using VITEK MS was 79.67, 76.42, and 76.42%, respectively. On the other hand, the two rapid methods generally achieved higher maximum and minimum S/N ratios with these isolates tested as compared to the routine method. Besides, the ZSB method produced overall mean of maximum and minimum S/N ratio higher than that by FUS. An in-house library of M-Discover MS was successfully built from 135 isolates from 42 species belonging to 18 genera using the ZSB method. Analysis of 467 isolates resulted in 97.22% correctly identified isolates to the species level by the ZSB method 95.50% by the routine method. The two novel methods are time- and cost-effective and allow efficient identification of filamentous fungi while providing a simplified procedure to build an in-house library. Thus, more clinical laboratories may consider adopting MALDI-TOF MS for filamentous fungi identification in the future.
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http://dx.doi.org/10.3389/fcimb.2021.687240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290857PMC
July 2021

Screening of Natural Phytocompounds Towards Identification of Potential Lead Compounds to Treat COVID-19.

Front Mol Biosci 2021 5;8:637122. Epub 2021 Jul 5.

Bioscience Research Foundation, Chennai, India.

COVID-19 is one of the members of the coronavirus family that can easily assail humans. As of now, 10 million people are infected and above two million people have died from COVID-19 globally. Over the past year, several researchers have made essential advances in discovering potential drugs. Up to now, no efficient drugs are available on the market. The present study aims to identify the potent phytocompounds from different medicinal plants (, , , , and ). In total, 227 phytocompounds were identified and screened against the proteins S-ACE2 and M through structure-based virtual screening approaches. Based on the binding affinity score, 30 active phytocompounds were selected. Amongst, the binding affinity for beta-sitosterol and beta-elemene against S-ACE2 showed -12.0 and -10.9 kcal/mol, respectively. Meanwhile, the binding affinity for beta-sitosterol and beta-chlorogenin against M was found to be -9.7 and -8.4 kcal/mol, respectively. Further, the selected compounds proceeded with molecular dynamics simulation, prime MM-GBSA analysis, and ADME/T property checks to understand the stability, interaction, conformational changes, binding free energy, and pharmaceutical relevant parameters. Moreover, the hotspot residues such as Lys31 and Lys353 for S-ACE2 and catalytic dyad His41 and Cys145 for M were actively involved in the inhibition of viral entry. From the in silico analyses, we anticipate that this work could be valuable to ongoing novel drug discovery with potential treatment for COVID-19.
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http://dx.doi.org/10.3389/fmolb.2021.637122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288047PMC
July 2021

Primary Pleural Squamous Cell Carcinoma, Highly Positive PD-L1, Shows Marked Response to Camrelizumab: A Case Report.

Clin Med Insights Oncol 2021 12;15:11795549211028571. Epub 2021 Jul 12.

Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan Province, China.

Here, we reported the rare case of primary pleural squamous cell carcinoma (PPSCC) in a 71-year-old male patient. After chemo and targeted therapies, the patient showed continuous tumor progression and clinical deterioration. Fortunately, the patient had a high expression level of PD-L1 (80%) in the tumor tissues. Ultimately, the patient survived for additional 6 months with camrelizumab treatment. In summary, camrelizumab may be a good candidate for the treatment of PPSCC, especially in tumors with high PD-L1 expression.
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http://dx.doi.org/10.1177/11795549211028571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278452PMC
July 2021

Unbalanced status and multidimensional influences of municipal solid waste management in Africa.

Chemosphere 2021 Oct 15;281:130884. Epub 2021 May 15.

Department of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.

The overall status of MSW management in Africa is evaluated systematically, in sight of life cycle indicators such as MSW generation daily per capita, collection and treatment methods. The relations of life cycle indictors with driving factors, including economic level, geographical location, urbanization and solid waste legislation process, are discussed. The results present that the major indicators had obvious regional differences, which can divide Africa into three levels: North Africa and South Africa > West Africa and Central Africa > East Africa. Besides, the disposal methods in North Africa, South Africa and West Africa have evolved from simple open dumping to sanitary landfill, composting and recycle treatment, while opening dumping is still rampant in East Africa. With a combination tool of Principal Component Analysis and Multiple Linear Regression, it was identified that economic growth, urbanization and geographical location are the most critical factors influencing the unbalanced statue of MSW management in Africa. By cluster analysis, unbalance status of MSW management in African countries can be integrated into three grades of MSW management level: good, fair and poor.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130884DOI Listing
October 2021

SMAD4 Inhibits Granulosa Cell Apoptosis via the miR-183-96-182 Cluster and FoxO1 Axis.

Reprod Sci 2021 Jul 21. Epub 2021 Jul 21.

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.

The miR-183-96-182 cluster is a polycistronic miRNA cluster necessary for ovarian functions in mammals. However, its transcriptional regulation in the ovary is largely unclear. In this study, we characterized the promoter region of the porcine miR-183-96-182 cluster, and showed that SMAD4 may function as a transcriptional activator of the miR-183-96-182 cluster in GCs through direct binding to SBE motifs in its promoter. SMAD4 may inhibit GC apoptosis via suppression of FoxO1, an effector of GC apoptosis and a direct target of the miR-183-96-182 cluster, by inducing the miR-183-96-182 cluster, and this process may be regulated by the TGF-β/SMAD signaling pathway. Our findings uncovered the regulatory mechanism of miR-183-96-182 cluster expression in GCs and demonstrated that TGF-β1/SMAD4/miR-183-96-182 cluster/FoxO1 may be a potential pathway for regulating follicular atresia and female reproduction.
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http://dx.doi.org/10.1007/s43032-021-00690-4DOI Listing
July 2021

Hypoglycemic and Hypolipidemic Activity of Fermented with YM 1301 in Diabetic C57BL/6 Mice.

J Med Food 2021 Jul;24(7):720-731

Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Science, Shaanxi Normal University, Xi'an, Shaanxi, China.

(PS) has been used as herbal medicine to treat type 2 diabetes mellitus (T2DM). However, how lactic acid fermentation of PS influences glucose and lipid metabolism remains unclear. The current study was undertaken to evaluate the hypoglycemic and hypolipidemic effects of PS fermented with YM 1301 (YM 1301) in streptozotocin and high-fat diet-induced T2DM mice. Biochemical analysis revealed that supplementation with metformin, PS, or fermented (FPS) lowered the fasting blood glucose, insulin, total cholesterol, triglyceride, and low-density lipoprotein cholesterol of diabetic mice. FPS showed relatively more potency to reduce the homeostasis model assessment-insulin resistance and glycated hemoglobin than PS. Moreover, a high dosage of FPS protected against glucose intolerance and insulin resistance by increasing the ratio of phosphor-AKT/AKT. Histological examination and quantitative polymerase chain reaction results showed that dietary FPS ameliorated the lipid accumulation in liver and white adipose tissue (WAT) by inhibiting lipogenesis, enhancing lipolysis, and fatty acid oxidation. FPS exhibited greater efficacy than PS on improving the transcriptional expression of adipose triacylglyceride lipase, carnitine palmitoyltransferase 1, and uncoupling protein 1. In addition, FPS exerted a striking anti-inflammatory effect by suppressing the expression of interleukin 6, interleukin 1, tumor necrosis factor-, and transforming growth factor- in WAT of diabetic C57BL/6 mice. Finally, FPS supplementation enhanced the activation of AMPK. In conclusion, these results suggest that the FPS may be more promising than PS as a potential therapeutic agent for diabetes and obesity.
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http://dx.doi.org/10.1089/jmf.2021.K.0034DOI Listing
July 2021

Epigenetic Regulation of Hepatic Stellate Cell Activation and Macrophage in Chronic Liver Inflammation.

Front Physiol 2021 1;12:683526. Epub 2021 Jul 1.

Department of Infectious Diseases, Renmin Hospital of Wuhan University, Wuhan, China.

Chronic liver inflammation is a complex pathological process under different stress conditions, and the roles of stellate cells and macrophages in chronic liver inflammation have been widely reported. Moderate liver inflammation can protect the liver from damage and facilitate the recovery of liver injury. However, an inflammatory response that is too intense can result in massive death of hepatocytes, which leads to irreversible damage to the liver parenchyma. Epigenetic regulation plays a key part in liver inflammation. This study reviews the regulation of epigenetics on stellate cells and macrophages to explore the new mechanisms of epigenetics on liver inflammation and provide new ideas for the treatment of liver disease.
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http://dx.doi.org/10.3389/fphys.2021.683526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281248PMC
July 2021

Long-term activity of tandem CD19/CD20 CAR therapy in refractory/relapsed B-cell lymphoma: a single-arm, phase 1-2 trial.

Leukemia 2021 Jul 16. Epub 2021 Jul 16.

Department of Bio-therapeutic, the First Medical Center, Chinese PLA General Hospital, Beijing, China.

Increasing the remission rate and reducing the recurrence rate can improve the clinical efficacy of chimeric antigen receptor (CAR) T cell therapy in recurrent/refractory non-Hodgkin lymphoma (r/rNHL). In this open-label, single-arm phase I/II trial, 87 patients with r/rNHL, including 58 patients with aggressive diffuse large B-cell lymphoma and 24 with high tumour burden, received an infusion at doses of 0.5 × 10-8 × 10 TanCAR7 T cells per kilogram of body weight after conditioning chemotherapy. The best overall response rate was 78% (95% confidence interval [CI], 68-86); response rates were consistent across prognostic subgroups. The median follow-up was 27.7 months. The median progression-free survival was 27.6 months (95% CI, 11 to not reached). Cytokine release syndrome (CRS) occurred in 61 patients (70%) with 60% of cases being grade 1 or 2 and 10% being grade 3 or greater. Grade 3 CAR T cell-related encephalopathy syndrome (CRES) occurred in 2 patients (2%). Two patients died from treatment-associated severe pulmonary infection, and one died from CRS-related pulmonary injury between 1 and 3 months post infusion. Long-term remissions were observed following the use of TanCAR7 T cells in r/rNHL with a safety profile that included CRS but few cases of CRES.
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http://dx.doi.org/10.1038/s41375-021-01345-8DOI Listing
July 2021

A clinical nomogram incorporating salivary level and oral hygiene index for predicting colorectal cancer.

Ann Transl Med 2021 May;9(9):754

Division of Gastroenterology and Hepatology, Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, State Key Laboratory for Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Institute of Digestive Disease, Shanghai, China.

Background: Emerging evidence demonstrates that the salivary microbiome could serve as a biomarker for various diseases. To date, the oral microbiome's role in the diagnosis of colorectal cancer (CRC) has not been fully elucidated. We aimed to illustrate the salivary microbiome's role in diagnosing and predicting the risk of CRC.

Methods: We collected preoperational saliva from 237 patients [95 healthy controls (HCs) and 142 CRC patients] who underwent surgical resections or colorectal endoscopy in Renji Hospital from January 2018 to January 2020. Clinical demographics, comorbidities, and oral health conditions were obtained from medical records or questionnaires. Salivary microbial biomarkers were detected using quantitative polymerase chain reaction (qPCR) after DNA extraction. Multivariate logistic regression analysis was employed to analyze the risk factors for CRC. A predictive model for the risk of developing CRC was constructed based on logistic regression analysis. Predictive accuracy was internally validated by bootstrap resampling. A clinical nomogram was constructed to visualize the predictive model.

Results: Logistic regression analysis demonstrated that the risk factors associated with CRC included age at diagnosis, male sex, poor oral hygiene, and relative salivary abundance. The predictive model had good discriminative (0.866) and calibration abilities (0.834) after bias correction.

Conclusions: The model based on age, sex, oral hygiene index (OHI), and the salivary level, which is visualized by a clinical nomogram, can predict the risk of CRC. Developing good oral hygiene habits might reduce the risk of CRC.
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http://dx.doi.org/10.21037/atm-20-8168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246182PMC
May 2021

HtrA4 is up-regulated during trophoblast syncytialization and BeWo cells fail to syncytialize without HtrA4.

Sci Rep 2021 Jul 13;11(1):14363. Epub 2021 Jul 13.

Implantation and Placental Development Laboratory, Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, VIC, 3168, Australia.

The outer layer of the human placenta comprises syncytiotrophoblast, which forms through fusion of cytotrophoblasts (syncytialization), and plays a critical role in maternal-fetal communication including nutrient/oxygen transportation and hormone secretion. Impairment in syncytialization inevitably affects pregnancy outcomes. High temperature requirement factor A 4 (HtrA4) is a placental-specific protease, expressed by various trophoblasts including syncytiotrophoblast, and significantly elevated in preeclampsia at disease presentation. However, it is unknown whether HtrA4 is important for syncytialization. Here we first examined HtrA4 expression in primary human cytotrophoblasts during syncytialization which occurs spontaneously in culture, and in BeWo cells which syncytialize upon forskolin stimulation. The success of syncytialization in each model was confirmed by significant up-regulation/secretion of β-hCG, and the concurrent down-regulation of E-cadherin. In both models, HtrA4 mRNA and protein increased concomitantly with syncytialization. Furthermore, the secreted levels of β-hCG and HtrA4 correlated significantly and positively in both models. We next knocked out HtrA4 in BeWo by CRISPR/Cas9. Upon forskolin treatment, control BeWo profoundly up-regulated β-hCG and syncytin-1, down-regulated E-cadherin, and at the same time increased the formation of multinucleated cells, whereas BeWo cells without HtrA4 did not alter any of these parameters. Our data thus suggest that HtrA4 plays an essential role in syncytialization.
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http://dx.doi.org/10.1038/s41598-021-93520-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277827PMC
July 2021

Transcription-coupled structural dynamics of topologically associating domains regulate replication origin efficiency.

Genome Biol 2021 Jul 12;22(1):206. Epub 2021 Jul 12.

State Key Laboratory of Membrane Biology, Biomedical Pioneer Innovation Center (BIOPIC), School of Life Sciences, Peking University, Beijing, 100871, China.

Background: Metazoan cells only utilize a small subset of the potential DNA replication origins to duplicate the whole genome in each cell cycle. Origin choice is linked to cell growth, differentiation, and replication stress. Although various genetic and epigenetic signatures have been linked to the replication efficiency of origins, there is no consensus on how the selection of origins is determined.

Results: We apply dual-color stochastic optical reconstruction microscopy (STORM) super-resolution imaging to map the spatial distribution of origins within individual topologically associating domains (TADs). We find that multiple replication origins initiate separately at the spatial boundary of a TAD at the beginning of the S phase. Intriguingly, while both high-efficiency and low-efficiency origins are distributed homogeneously in the TAD during the G1 phase, high-efficiency origins relocate to the TAD periphery before the S phase. Origin relocalization is dependent on both transcription and CTCF-mediated chromatin structure. Further, we observe that the replication machinery protein PCNA forms immobile clusters around TADs at the G1/S transition, explaining why origins at the TAD periphery are preferentially fired.

Conclusion: Our work reveals a new origin selection mechanism that the replication efficiency of origins is determined by their physical distribution in the chromatin domain, which undergoes a transcription-dependent structural re-organization process. Our model explains the complex links between replication origin efficiency and many genetic and epigenetic signatures that mark active transcription. The coordination between DNA replication, transcription, and chromatin organization inside individual TADs also provides new insights into the biological functions of sub-domain chromatin structural dynamics.
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http://dx.doi.org/10.1186/s13059-021-02424-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276456PMC
July 2021

Experimental Research on the Effect of Ultrasonic Waves on the Adsorption, Desorption, and Seepage Characteristics of Shale Gas.

ACS Omega 2021 Jul 24;6(26):17002-17018. Epub 2021 Jun 24.

State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation, Southwest Petroleum University, Chengdu 610500, Sichuan, P. R. China.

Shale gas reservoirs are tight reservoirs with ultralow porosity and ultralow permeability, and their matrix pores are mostly nanoscale. In addition, matrix particles and organic pore surfaces adsorb shale gas. These problems cause the production per well of shale gas to be lower than that of conventional natural gas. The use of hydraulic fracturing technology to exploit shale gas can achieve a good production increase effect. However, using this technology has some limitations caused by technical characteristics and geological conditions. Therefore, new technologies for shale gas exploitation need to be explored. In this study, we propose a method to improve the flow characteristics of shale gas by using ultrasonic waves to increase shale gas production and perform experimental tests to research the actual effect of this method. The lithology, mineral composition, pore structure, specific surface area, and pore size distribution of shale samples are tested. Then, the attenuation characteristics of ultrasonic waves propagating in shale are analyzed. Finally, the effect of ultrasonic waves on the adsorption, desorption, and seepage of shale gas is explored. Results show that the Langmuir adsorption isotherm can describe the adsorption characteristics of shale gas under the action of ultrasonic waves. The gas adsorption constant decreases with increasing ultrasonic wave power. The ultrasonic waves accelerate the gas desorption rate, significantly increase the desorption volume, and prolong the time taken to reach desorption equilibrium. They also increase the permeability of shale gas, and the growth is proportional to the power of the ultrasonic waves. These results indicate that the permeability of shale gas has a power function relationship with the effective stress under ultrasonic waves.
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http://dx.doi.org/10.1021/acsomega.1c02018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264936PMC
July 2021

Correlated vibration-solvent effects on the non-Condon exciton spectroscopy.

J Chem Phys 2021 Jun;154(24):244105

Department of Chemical Physics, University of Science and Technology of China, Hefei, Anhui 230026, China.

Excitation energy transfer is crucially involved in a variety of systems. During the process, the non-Condon vibronic coupling and the surrounding solvent interaction may synergetically play important roles. In this work, we study the correlated vibration-solvent influences on the non-Condon exciton spectroscopy. Statistical analysis is elaborated for the overall vibration-plus-solvent environmental effects. Analytic solutions are derived for the linear absorption of monomer systems. General simulations are accurately carried out via the dissipaton-equation-of-motion approach. The resulted spectra in either the linear absorption or strong field regime clearly demonstrate the coherence enhancement due to the synergetic vibration-solvent correlation.
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http://dx.doi.org/10.1063/5.0053169DOI Listing
June 2021

Rational Design of Single-Atom Site Electrocatalysts: From Theoretical Understandings to Practical Applications.

Adv Mater 2021 Jul 8:e2008151. Epub 2021 Jul 8.

Department of Chemistry, Tsinghua University, Beijing, 100084, China.

Atomically dispersed metal-based electrocatalysts have attracted increasing attention due to their nearly 100% atomic utilization and excellent catalytic performance. However, current fundamental comprehension and summaries to reveal the underlying relationship between single-atom site electrocatalysts (SACs) and corresponding catalytic application are rarely reported. Herein, the fundamental understandings and intrinsic mechanisms underlying SACs and corresponding electrocatalytic applications are systemically summarized. Different preparation strategies are presented to reveal the synthetic strategies with engineering the well-defined SACs on the basis of theoretical principle (size effect, metal-support interactions, electronic structure effect, and coordination environment effect). Then, an overview of the electrocatalytic applications is presented, including oxygen reduction reaction, hydrogen evolution reaction, oxygen evolution reaction, oxidation of small organic molecules, carbon dioxide reduction reaction, and nitrogen reduction reaction. The underlying structure-performance relationship between SACs and electrocatalytic reactions is also discussed in depth to expound the enhancement mechanisms. Finally, a summary is provided and a perspective supplied to demonstrate the current challenges and opportunities for rational designing, synthesizing, and modulating the advanced SACs toward electrocatalytic reactions.
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http://dx.doi.org/10.1002/adma.202008151DOI Listing
July 2021

Clinical Characteristics and Visual Outcomes in Patients with Intralenticular Foreign Bodies with Self-Sealing Corneal Penetrating Wounds.

J Ophthalmol 2021 21;2021:6613205. Epub 2021 Jun 21.

Eye Center, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.

Purpose: Siderosis bulbi may occur as a result of retained intralenticular foreign bodies (ILFBs) that were missed during examination in patients with self-sealing wounds and without a significant decrease in visual acuity. This study aimed to explore the clinical characteristics and visual outcomes of ILFBs with self-sealing corneal penetrating wounds.

Methods: Fifteen eyes of 15 patients with ILFBs and self-sealing corneal penetrating wounds, seen between October 2014 and September 2019, were retrospectively analyzed. Data regarding the patient demographics, clinical features, surgical procedure, and initial and final best-corrected visual acuity (BCVA) were analyzed.

Results: All patients were male with a mean age of 41 years. The foreign bodies passed through the cornea, sometimes through the iris, through the anterior capsule, and finally localized in the lens. All ILFBs were pointed and metallic objects and were successfully removed with phacoemulsification and posterior chamber intraocular lens (IOL) implantation. Anterior capsule violation was found in three eyes, but no posterior capsule rupture was found. The IOL was placed in a capsule bag in all the cases. The BCVA ranged from 20/200 to 20/25 preoperatively and improved to between 20/32 and 20/20 at the last follow-up visit. The IOLs were well-centered. Apart from posterior capsule opacity in four eyes, no other postoperative complications were found.

Conclusions: In patients with a pointed metallic ILFB and self-sealing corneal penetrating wounds (with or without cataracts), early diagnosis and removal of the metallic ILFB combined with lens removal and IOL implantation may avoid late complications and achieve good visual outcomes.
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http://dx.doi.org/10.1155/2021/6613205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241495PMC
June 2021

GSTP1 as a novel target in radiation induced lung injury.

J Transl Med 2021 Jul 8;19(1):297. Epub 2021 Jul 8.

Department of Radiation Oncology, The Fifth Medical Center of the Chinese PLA General Hospital , Beijing, China.

The glutathione S-transferase P1(GSTP1) is an isoenzyme in the glutathione-S transferases (GSTs) enzyme system, which is the most abundant GSTs expressed in adult lungs. Recent research shows that GSTP1 is closely related to the regulation of cell oxidative stress, inhibition of cell apoptosis and promotion of cytotoxic metabolism. Interestingly, there is evidence that GSTP1 single nucleotide polymorphisms (SNP) 105Ile/Val related to the risk of radiation induced lung injury (RILI) development, which strongly suggests that GSTP1 is closely associated with the occurrence and development of RILI. In this review, we discuss our understanding of the role of GSTP1 in RILI and its possible mechanism.
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http://dx.doi.org/10.1186/s12967-021-02978-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268607PMC
July 2021

Fostering green development with green finance: An empirical study on the environmental effect of green credit policy in China.

J Environ Manage 2021 Jul 5;296:113159. Epub 2021 Jul 5.

Center for Energy and Environmental Policy Research, Beijing Institute of Technology, Beijing, 100081, China; School of Management and Economics, Beijing Institute of Technology, Beijing, 100081, China; Beijing Key Lab of Energy Economics and Environmental Management, Beijing, 100081, China; Sustainable Development Research Institute for Economy and Society of Beijing, Beijing, 100081, China; Collaborative Innovation Center of Electric Vehicles in Beijing, Beijing, 100081, China. Electronic address:

To direct financial resources to cleaner production enterprises and achieve the goal of environmental governance, the Chinese government has devoted increasing efforts to facilitating green finance. As one of the major policies of green finance, the Green Credit Policy (GCP) was issued in 2012. Evaluating whether the GCP can promote green development has important significance, but few studies have explored its policy effects for the investment and financing behavior of "two high" (high energy consumption and high pollution) enterprises and environmental quality from both micro and macro perspectives. Taking the promulgation of the GCP as a quasi-natural experiment, based on a panel dataset involving 945 A-share listed companies and 30 provinces for the period of 2004-2017, this paper adopts the difference-in-difference model to explore the investment and financing behavior changes of enterprises and environmental impacts of the GCP. The following conclusions are derived. (1) The GCP provides incentives for the short-term financing behavior of "two high" enterprises, but it has a punitive effect in the long term and significantly inhibits the investment behavior of such enterprises. (2) The GCP contributes to the mitigation of sulfur dioxide and wastewater emissions. (3) The GCP has a greater effect on investment and financing behavior among state-owned and large-scale "two high" enterprises than among medium-sized and micro enterprises. (4) There exists regional heterogeneity in the effects of the GCP on the investment and financing of "two high" enterprises and environmental quality. The GCP has positive impacts in the eastern and western regions, and the policy effect is not obvious in the central region.
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http://dx.doi.org/10.1016/j.jenvman.2021.113159DOI Listing
July 2021

Intrinsic Room-Temperature Ferromagnetism in VC MXene Nanosheets.

ACS Appl Mater Interfaces 2021 Jul 8;13(28):33363-33370. Epub 2021 Jul 8.

National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230029, P. R. China.

Two-dimensional (2D) materials with intrinsic magnetic properties are intensively explored due to their potential applications in low-power-consumption electronics and spintronics. To date, only a handful of intrinsic magnetic 2D materials have been reported. Here, we report a realization of intrinsic ferromagnetic behavior in 2D VC MXene nanosheets through layer mismatch engineering. The VC MXene nanosheets with a small-angle twisting show a robust intrinsic ferromagnetic response with a saturation magnetic moment of 0.013 emu/g at room temperature. An in-depth study has been performed by X-ray absorption spectroscopy as well as electron paramagnetic resonance (EPR) and photoelectron spectroscopy analyses. It has been revealed that the symmetry-broken interlayer twisting reduced the degeneracy of V 3d states and the van Hove singularity. This led to a redistribution of the density of electronic states near the Fermi level and consequently activated the Stoner ferromagnetism with improved density of itinerant d electrons. This work highlights VC MXene as a promising intrinsic room-temperature ferromagnetic material with potential applications in spintronics or spin-based electronics.
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http://dx.doi.org/10.1021/acsami.1c07906DOI Listing
July 2021

DctR contributes to the virulence of avian pathogenic Escherichia coli through regulation of type III secretion system 2 expression.

Vet Res 2021 Jul 6;52(1):101. Epub 2021 Jul 6.

Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.

Pathogens could precisely alter their gene expression to facilitate their survival and successful infection. The LuxR family transcriptional regulator DctR (also known as YhiF) was shown to participate in the regulation of acid fitness and adhesion of enterohemorrhagic E. coli (EHEC) O157:H7. Avian pathogenic Escherichia coli (APEC) causes significant economic losses to the poultry industries and also potentially threatens human health. However, the effects of DctR on the fitness and virulence of APEC have not been investigated yet. To assess the function of DctR in APEC, the dctR gene mutant and complemented strains were constructed and biologically characterized. Our results show that inactivation of the dctR gene led to decreased biofilm formation, diminished serum resistance, reduced adherence capacity, attenuated colonization and virulence of APEC in ducks. The altered capacities of the mutant strain were restored by genetic complementation. In addition, we found that DctR positively regulates the expression of E. coli type III secretion system 2 (ETT2) core genes in APEC. The expression of the inflammatory cytokines interleukin (IL)-1β and IL-8 were decreased in HD-11 macrophages infected with the mutant strain compared with the wild-type strain. These observations indicate that regulator DctR contributes to the virulence of APEC through regulation of ETT2 expression.
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http://dx.doi.org/10.1186/s13567-021-00970-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8259166PMC
July 2021

Comparison of biological and transcriptomic effects of conventional cigarette and electronic cigarette smoke exposure at toxicological dose in BEAS-2B cells.

Ecotoxicol Environ Saf 2021 Jul 3;222:112472. Epub 2021 Jul 3.

School of Pharmaceutical Sciences, National and Local United Engineering Lab of Druggability and New Drugs Evaluation, Sun Yat-sen University, Guangzhou, Guangdong 510006, China. Electronic address:

Cigarette seriously affects human health, and electronic cigarette (e-cigarette), considered as cigarette substitutes, become popular as its contribution to quit smoking. But scientific evidence about the absolute safety of e-cigarette is insufficient. Previous studies also have indicated that different dosages of cigarette can lead to different biological effects. Thus, the impact of cigarette at toxicological dose such as IC50 compared with that of e-cigarette are highly needed. In this study, we investigated the effects of cigarette smoke condensate (CSC) at toxicological dose compared with e-cigarette smoke condensate (ECSC) in equivalent nicotine level. Nicotine content of CSC and ECSC were determined by UPLC. Human lung epithelial cells (BEAS-2B) were exposed to 0-32 μg/ml of CSC and ECSC for 24 h to determine IC50 of cell viability and morphological assessment. Inflammation, apoptosis, cell cycle analysis and RNA-Seq transcriptome analysis were performed to characterize the differences between CSC and ECSC. We found that acute exposure of BEAS-2B cells to CSC at IC50 leaded to morphological change, inflammatory cytokines production and cell apoptosis, while ECSC did not exert such cell effects in equivalent nicotine level. The transcriptome analysis showed that differentially expressed genes in CSC were far more than that in ECSC, and mainly enriched in the category of cell cycle, DNA repair, cancer, and metabolic related pathways. Such cell cycle arrest was further experimentally confirmed. These results suggested that toxicological dose of ECSC might be much higher than that of CSC. Based on equivalent nicotine content, an acute exposure to CSC had significant impacts on cell effects and gene expression profile compared to ECSC. Our results provided a reference for the safety studies of conventional cigarette and e-cigarette.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112472DOI Listing
July 2021

Anti-PD-1 antibodies as a salvage therapy for patients with diffuse large B cell lymphoma who progressed/relapsed after CART19/20 therapy.

J Hematol Oncol 2021 Jul 5;14(1):106. Epub 2021 Jul 5.

Department of Bio-Therapeutic, the First Medical Centre in Chinese PLA General Hospital, Beijing, China.

CD19-directed chimeric antigen receptor T cell (CART19) therapy is efficient and approved for relapsed/refractory diffuse large B cell lymphoma (DLBCL). To increase durable antitumor response, we previously designed tandem CART19/20 cells and shown longer progression-free survival. However, a proportion of CART19/20-treated patients will finally progress and require salvage therapies. In this study, we analyzed data from five patients with relapsed/refractory DLBCL who had disease progression or relapse following CART19/20 therapy and then treated with PD-1-blocking antibodies as salvage therapy. Two of five patients acquired complete remissions after anti-PD-1 therapy, including one patient remained ongoing remission for more than 21 months. One patient achieved a partial remission, and the other two had progressive diseases. No ≥ grade 3 treatment-related adverse events or cytokine release syndrome was observed. Immunohistochemistry of tumor specimens revealed higher PD-1/PD-L1 expression in responsive patients with anti-PD-1 therapy as compared to that in non-responders. After anti-PD-1 treatment, circulating T cells were activated in responders, and no significant expansion of CART19/20 cells was detected. Our data suggest that PD-1 blockade therapy can be active in patients with relapsed/refractory DLBCL after failure of CAR T cell therapy who had PD-L1 expression in tumor cells and high PD-1 level in tumor-infiltrated T cells.
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http://dx.doi.org/10.1186/s13045-021-01120-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8259370PMC
July 2021

Detection of Fuchs' Uveitis Syndrome From Slit-Lamp Images Using Deep Convolutional Neural Networks in a Chinese Population.

Front Cell Dev Biol 2021 18;9:684522. Epub 2021 Jun 18.

The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology and Chongqing Eye Institute, Chongqing Branch of National Clinical Research Center for Ocular Diseases, Chongqing, China.

Fuchs' uveitis syndrome (FUS) is one of the most under- or misdiagnosed uveitis entities. Many undiagnosed FUS patients are unnecessarily overtreated with anti-inflammatory drugs, which may lead to serious complications. To offer assistance for ophthalmologists in the screening and diagnosis of FUS, we developed seven deep convolutional neural networks (DCNNs) to detect FUS using slit-lamp images. We also proposed a new optimized model with a mixed "attention" module to improve test accuracy. In the same independent set, we compared the performance between these DCNNs and ophthalmologists in detecting FUS. Seven different network models, including Xception, Resnet50, SE-Resnet50, ResNext50, SE-ResNext50, ST-ResNext50, and SET-ResNext50, were used to predict FUS automatically with the area under the receiver operating characteristic curves (AUCs) that ranged from 0.951 to 0.977. Our proposed SET-ResNext50 model (accuracy = 0.930; Precision = 0.918; Recall = 0.923; F1 measure = 0.920) with an AUC of 0.977 consistently outperformed the other networks and outperformed general ophthalmologists by a large margin. Heat-map visualizations of the SET-ResNext50 were provided to identify the target areas in the slit-lamp images. In conclusion, we confirmed that a trained classification method based on DCNNs achieved high effectiveness in distinguishing FUS from other forms of anterior uveitis. The performance of the DCNNs was better than that of general ophthalmologists and could be of value in the diagnosis of FUS.
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http://dx.doi.org/10.3389/fcell.2021.684522DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250145PMC
June 2021

Depressive symptoms, anxiety symptoms, and their co-occurrence among people living with schizophrenia in China: Prevalence and correlates.

J Clin Psychol 2021 Jul 2. Epub 2021 Jul 2.

Department of Social Medicine and Health Management, Xiangya School of Public Health, Central South University, Changsha, Hunan, China.

Background: The current study examines the prevalence of depressive symptoms, anxiety symptoms, their occurrence, and key socio-demographic and clinical correlates among people living with schizophrenia.

Methods: A cross-sectional study was conducted on 390 schizophrenia individuals. Depressive and anxiety symptoms, patient symptoms, functioning, and disability were assessed using standard assessment tools.

Results: People living with schizophrenia had a prevalence of 40.51% for depressive symptoms, 29.74% for anxiety symptoms, and 26.41% for their co-occurrence. More symptoms (odds ratio [OR]: 1.04-1.06, 95% confidence interval [CI]: 1.01-1.10) and higher disability (OR: 1.06, 95% CI: 1.03-1.09) were associated with increased risk of depressive symptoms, anxiety symptoms, and their co-occurrence. In addition, having middle school or high school education (OR: 2.48-2.61, 95% CI: 1.15-5.53), and being unemployed (OR: 4.98-9.08, 95% CI: 1.09-69.87) were associated with increased risk for anxiety symptoms and its co-occurrence with depressive symptoms.

Conclusions: Depressive and anxiety symptoms are relatively common. Interventions should carefully assess these symptoms to distinguish them from schizophrenia to target them in the treatment.
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http://dx.doi.org/10.1002/jclp.23141DOI Listing
July 2021
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