Publications by authors named "Yao Sun"

574 Publications

Allogeneic haematopoietic stem cell transplantation with decitabine-containing preconditioning regimen in TP53-mutant myelodysplastic syndromes: A case study.

Front Oncol 2022 18;12:928324. Epub 2022 Jul 18.

Senior Department of Hematology, The Fifth Medical Centre of Chinese People's Liberation Army General Hospital, Beijing, China.

Myelodysplastic syndrome (MDS) with TP53 mutations has a poor prognosis after transplantation, and novel therapeutic means are urgently needed. Decitabine (Dec) monotherapy has demonstrated improved overall response rates in MDS and acute myeloid leukaemia, although these responses were not durable. This study aimed to preliminary evaluate the efficacy of a Dec-containing allogeneic haematopoietic stem cell transplantation (allo-HSCT) preconditioning regimen in TP53-mutant MDS. Nine patients with TP53-mutant myelodysplastic syndromes received the decitabine-containing preconditioning regimen and subsequent myeloablative allo-HCT between April 2013 and September 2021 in different centres. At a median follow-up of 42 months (range, 5 to 61 months), the overall survival (OS) was 89% (8/9), progression-free survival (PFS) was 89% (8/9), and relapse incidence was 11.1%. The incidence of severe acute (grade III-IV) graft-versus-host disease (GVHD) was 22.2% (2/9) and that of chronic moderate-to-severe GVHD was 11.1% (1/9). The 1-year GVHD-free/relapse-free survival (GRFS) was 56% (5/9). In conclusion, we found real-world clinical data that supports the use of a Dec-containing preconditioning regimen before allo-HSCT for possible improved outcomes in TP53-mutant MDS patients; there is therefore an urgent call for an in-depth exploration of the involved mechanism to confirm these preliminary findings.
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http://dx.doi.org/10.3389/fonc.2022.928324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339648PMC
July 2022

Long wavelength-emissive Ru(II) metallacycle-based photosensitizer assisting in vivo bacterial diagnosis and antibacterial treatment.

Proc Natl Acad Sci U S A 2022 Aug 1;119(32):e2209904119. Epub 2022 Aug 1.

Key Laboratory of Pesticides and Chemical Biology, Ministry of Education, International Joint Research Center for Intelligent Biosensor Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, China.

Ruthenium (Ru) complexes are developed as latent emissive photosensitizers for cancer and pathogen photodiagnosis and therapy. Nevertheless, most existing Ru complexes are limited as photosensitizers in terms of short excitation and emission wavelengths. Herein, we present an emissive Ru(II) metallacycle (herein referred to as 1) that is excited by 808-nm laser and emits at a wavelength of ∼1,000 nm via coordination-driven self-assembly. Metallacycle 1 exhibits good optical penetration (∼7 mm) and satisfactory reactive oxygen species production properties. Furthermore, 1 shows broad-spectrum antibacterial activity (including against drug-resistant ) as well as low cytotoxicity to normal mammalian cells. In vivo studies reveal that 1 is employed in precise, second near-infrared biomedical window fluorescent imaging-guided, photo-triggered treatments in -infected mice models, with negligible side effects. This work thus broads the applications of supramolecular photosensitizers through the strategy of lengthening their wavelengths.
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http://dx.doi.org/10.1073/pnas.2209904119DOI Listing
August 2022

How Well Are Socioeconomic Factors Associated With Improved Outcomes for Infants Diagnosed With Early Childhood Developmental Delay? An Observational Study.

Front Pediatr 2022 12;10:890719. Epub 2022 Jul 12.

Department of Rehabilitation Medicine, Children's Hospital of Nanjing Medical University, Nanjing, China.

Purpose: Early childhood developmental delay remains problematic worldwide in terms of weight and the five domains of child development, including gross motor, fine motor, cognition, language, and social domains. Based on the World Health Organization (WHO) guideline and the theoretical domain framework, this study identified five key socioeconomic factors, such as parenting time during hospitalization, parental educational level, medical spending, distance to hospital, and medical insurance coverage, to describe how these five factors are associated with improved outcomes of developmental quotient (DQ) values and the weight of infants in a tertiary hospital.

Methods: In this prospective observational study, clinical and socioeconomic data were collected. Clinical data included the weight and DQ values of infants and other data relevant to the birth of infants. A National Developmental Scale was used to observe infants in five domains and calculate the DQ values of infants. These five domains include gross motor, fine motor, cognition, language, and social domains. Parenting time during hospitalizations was observed by a research nurse. Other socioeconomic factors were reported by parents and verified with system information.

Results: A total of 75 infants' parents were approached, of which 60 were recruited. The age of infants ranged from 75 to 274 days at the first admission. Increments of their weight and DQ values improved from -0.5 to 2.5 kg and from -13 to 63, respectively. More than half of the parents (54.1%) were at the level of minimum secondary education although the results were not statistically significant. However, there was a positive correlation between weight improvement and parenting time during hospitalization ((58) = 0.258, < 0.05), medical spending ((58) = 0.327, < 0.05), distance to hospital ((58) = 0.340, < 0.01), but there was a negative association with medical insurance coverage ((58) =-0.256, < 0.05). There was also a significant relationship between the improved DQ value and distance to hospital ((58)= 0.424, < 0.01).

Conclusion: Parenting time during hospitalization, medical spending, distance to hospital, and medical insurance coverage are important factors for early childhood developmental delay in relation to possible hospital intervention and improved accessibility to health services for families in rural areas. Therefore, changes in the current medical scheme are needed because a universal medical subsidy among regions will reduce the financial burden of families and provide families with more access to the necessary health services that their children need.
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http://dx.doi.org/10.3389/fped.2022.890719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315099PMC
July 2022

Rationally designed Ru(II) metallacycles with tunable imidazole ligands for synergistical chemo-phototherapy of cancer.

Chem Commun (Camb) 2022 Jul 27. Epub 2022 Jul 27.

Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China.

Herein, we construct a series of Ru(II) metallacycles with multimodal chemo-phototherapeutic properties, which exhibited much higher anticancer activity and better cancer-cell selectivity than cisplatin. The antitumor mechanism could be ascribed to the activation of caspase 3/7 and the resulting apoptosis. These results open new possibilities for Ru(II) metallacycles in biomedicine.
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http://dx.doi.org/10.1039/d2cc03118cDOI Listing
July 2022

Prognostic role of PD-L1 expression in patients with salivary gland carcinoma: A systematic review and meta-analysis.

PLoS One 2022 26;17(7):e0272080. Epub 2022 Jul 26.

Department of Stomatology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.

Background: Although programmed cell death-ligand 1 (PD-L1) has been recognized as a potential marker in several cancers, the relationship between PD-L1 expression and survival in patients with salivary gland carcinoma (SGC) has remained unclear. We aimed to evaluate the association of PD-L1 expression with clinicopathological features and prognosis in SGC patients.

Methods: The databases Ovid Medline, PubMed, Scopus, and EMBASE were searched for relevant studies that detected PD-L1 expression in SGC. The meta-analysis was performed according to the preferred reporting items for systematic reviews and meta-analyses (PRISMA), and the reporting recommendations for tumor marker prognostic studies (REMARK) was used to assess the quality of research eligible for this meta-analysis. Included studies were assessed using the Quality in Prognosis Studies (QUIPS) tool. Odds ratios (ORs) with 95% confidence interval (CI) were calculated to estimate the correlation between PD-L1 expression and clinicopathological features. Hazard ratios (HRs) with 95% CI were applied to assess the association between PD-L1 expression and survival outcomes of patients.

Results: A total of ten studies (including 952 patients with SGC) were evaluated. The meta-analysis showed that positive PD-L1 expression in SGC was significantly associated with male patients, older age, Tumor stage, lymph node metastasis, high pathological grade, and non-adenoid cystic carcinoma subtype. The pooled data demonstrated that high PD-L1 expression was associated with poor overall survival and disease-free survival. There was no significant correlation between PD-L1 expression and progression-free survival or disease-specific survival of SGC patients.

Conclusion: According to the meta-analysis, positive PD-L1 expression may play an important role as an effective marker of poor prognosis in patients with SGC. However, large-scale, prospective investigations are still needed to confirm the findings. The assessment of PD-L1 expression may aid in the personalized management of SGC.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0272080PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321421PMC
July 2022

Accuracy of a chairside, fused deposition modeling three-dimensional-printed, single tooth surgical guide for implant placement: A randomized controlled clinical trial.

Clin Oral Implants Res 2022 Jul 19. Epub 2022 Jul 19.

Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China.

Purpose: To compare the accuracy of chairside, fused deposition modeling (FDM) three-dimensional (3D)-printed surgical guides with that of stereolithographic guides for implant placement in single edentulous sites within a clinical setting.

Materials And Methods: A total of 28 participants with 30 single posterior edentulous sites were included. The sites were randomized into a FDM 3D-printed surgical guide group (test) or stereolithographic guide group (control) of equal size (n = 15). In both groups, digital implant planning was performed using data from cone beam-computed tomography and intraoral scans. The test group's surgical guides were fabricated using a chairside, FDM 3D-printer; those in the control group were fabricated using a light-curing 3D-printer. Postoperative intraoral scans were used to obtain the 3D position of the implants. Compared to preoperative design, the angular, 3D, mesiodistal, buccolingual and apicocoronal deviations at the implant shoulder and apex were recorded.

Results: The workflow for the design and chairside fabrication of implant guides was established. The mean angular deviations of the test and control group were (4.23 ± 2.38) ° and (4.13 ± 2.42) ° (p > .05), respectively. The respective 3D deviations at the implant shoulder were (0.70 ± 0.44) mm and (0.55 ± 0.27) mm (p > .05); those at the implant apex were (1.25 ± 0.61) mm and (1.11 ± 0.54) mm (p > .05). The mesiodistal, buccolingual, and apicocoronal deviations at the implant shoulder and apex did not significantly differ between the groups (p > .05).

Conclusions: Implants for single posterior edentulous spaces were placed as accurately with the test guide as with the control. Further research under more complex situations involving multiple missing teeth is needed.
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http://dx.doi.org/10.1111/clr.13981DOI Listing
July 2022

High-Efficiency Treatment for Osteoarthritis Self-Assembled Dual-Functionalized Nanobiologics.

ACS Biomater Sci Eng 2022 Aug 8;8(8):3320-3328. Epub 2022 Jul 8.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.

Osteoarthritis (OA) is a progressive joint disease that has a complex pathogenesis and lacks effective drugs. OA develops with cartilage degeneration and inflammation, thus synthesizing a drug with both anti-inflammatory properties and cartilage-repair capacity provides a promising treatment strategy. Therefore, in this study, we report self-assembled nanobiologics composed of an engineered recombinant IL-1 receptor antagonist (IL-1Ra) chimeric protein with chondroitin sulfate (CS). The nanobiologics, termed ICN, exhibit extraordinary biocompatibility, low immunogenicity, and good bioefficacy. Furthermore, our study revealed that ICN significantly reduced cartilage degradation, inhibited synovial inflammation, and suppressed osteophyte formation in OA rat models. The excellent therapeutic effects on OA can be attributed to the synergistic anti-inflammatory and cartilage-repair properties of ICN's constituents. Thus, our novel strategy offers insights into the development of drugs for OA treatment and research on nanobiomedicine, which can also be adapted for other diseases with similar pathologies.
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http://dx.doi.org/10.1021/acsbiomaterials.2c00332DOI Listing
August 2022

Metformin Can Enhance the Inhibitory Effect of Olaparib in Bladder Cancer Cells.

Dis Markers 2022 24;2022:5709259. Epub 2022 Jun 24.

Basic Medical College, Jiamusi University, China.

Background: Bladder cancer is a common urinary system tumor. In the treatment of clinical patients, it is particularly important to find an effective treatment method to inhibit tumor growth. The world's first PARP inhibitor olaparib is mainly used for the treatment of BRCA1/BRCA2 mutated tumors. Metformin, an antidiabetic drug, has been reported to reduce cancer incidence in humans and improve survival in cancer patients.

Methods: Cell viability and proliferation were detected by CCK-8 assay and colony formation assay; cell apoptosis was detected by flow cytometry; cell migration and invasion abilities were detected by scratch assay and Transwell assay; STAT3/C-MYC signaling pathway protein were detected by western blotting.

Results: Olaparib combined with metformin has better effects on the proliferation, clone formation, migration, invasion, and apoptosis of bladder cancer cells than single drug, indicating that metformin can enhance the inhibitory effect of olaparib on tumor growth and regulate the expression of STAT3/C-MYC signaling pathway proteins.

Conclusion: The results of this study showed that metformin could significantly enhance the antitumor effect of olaparib on bladder cancer cells, and these effects were mediated by downregulating STAT3/C-MYC signaling pathway proteins. This finding may have potential clinical application in the treatment of bladder cancer.
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http://dx.doi.org/10.1155/2022/5709259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249502PMC
July 2022

Rationally designed Ru(ii)-metallacycle chemo-phototheranostic that emits beyond 1000 nm.

Chem Sci 2022 Jun 18;13(22):6541-6549. Epub 2022 May 18.

Key Laboratory of Pesticides and Chemical Biology, Ministry of Education, International Joint Research Center for Intelligent Biosensor Technology and Health, College of Chemistry, Central China Normal University Wuhan 430079 China

Ruthenium complexes are emerging as potential complements to platinum drugs. They also show promise as photo-diagnostic and therapeutic agents. However, most ruthenium species studied to date as potential drugs are characterized by short excitation/emission wavelengths. This limits their applicability for deep-tissue fluorescence imaging and light-based therapeutic treatments. Here, we report a Ru(ii) metallacycle (Ru1100) that emits at ≥1000 nm. This system possesses excellent deep-tissue penetration capability (∼7 mm) and displays good chemo-phototherapeutic performance. studies revealed that Ru1100 benefits from good cellular uptake and produces a strong anticancer response against several cancer cell lines, including a cisplatin-resistant A549 cell line (IC = 1.6 μM 51.4 μM for cisplatin). On the basis of studies, it is concluded that Ru1100 exerts its anticancer action by regulating cell cycle progression and triggering cancer cell apoptosis. studies involving the use of a nanoparticle formulation served to confirm that Ru1100 allows for high-performance NIR-II fluorescence imaging-guided precise chemo-phototherapy in the case of A549 tumour mouse xenografts with no obvious side effects. This work thus provides a paradigm for the development of long-wavelength emissive supramolecular theranostic agents based on ruthenium.
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http://dx.doi.org/10.1039/d2sc01518hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9172562PMC
June 2022

Activation of farnesoid X receptor signaling by geniposidic acid promotes osteogenesis.

Phytomedicine 2022 Aug 9;103:154258. Epub 2022 Jun 9.

Key Laboratory of Big Data-Based Precision Medicine, School of Engineering Medicine, Beihang University, Beijing, 100191, China; Clinical Research Platform for Interdiscipline of Stomatology, The First Affiliated Hospital of Jinan University & Department of Stomatology, Jinan University, Guangzhou, 510632, China. Electronic address:

Background: New targets and strategies are urgently needed for the identification and development of anabolic drugs for osteoporosis. Farnesoid X receptor (FXR) is a promising novel therapeutic target for bone metabolism diseases. Although used clinically, FXR agonists have obvious side effects; therefore, the development of new FXR agonists for the treatment of osteoporosis would be welcomed. Geniposidic acid (GPA) is a bioactive compound extracted from Eucommiae cortex, which is used for treating arthritis, osteoporotic fractures, and hypertension. However, the therapeutic effects of GPA against osteoporosis remain underexplored.

Purpose: This study aims to reveal the potential osteogenic effects of FXR and to explore the effect of GPA on bone formation, osteoporosis treatment, and FXR signaling.

Study Design & Methods: The role of FXR in promoting bone formation was evaluated in Fxr knockout (Fxr) mice and cell models. GPA activation of FXR was evaluated by molecular docking and luciferase reporter gene assays. Thirty female C57BL/6J mice were randomly assigned into a sham operation group (Sham) and four ovariectomized (OVX) groups (n=6 each) and were treated with vehicle or different doses of GPA (25, 50, and 100 mg/kg/day). The therapeutic effect of GPA on osteoporosis was systematically analyzed by performing bone histomorphometry and measuring serum biochemical parameters, and the molecular mechanism was also evaluated. Furthermore, the action of GPA in Fxr mice was evaluated to investigate its dependency on FXR in promoting bone formation and treating osteoporosis.

Results: We found that FXR was highly expressed in bone tissues and enriched in osteoblasts. Notably, deletion of FXR significantly reduced the bone formation rate and bone mass of the Fxr mice compared with wild-type mice. Furthermore, using a high throughput drug screening strategy based on fluorescent reporter genes, we found that GPA functions as a natural agonist of FXR. We confirmed the activities of GPA on FXR activation and osteogenesis in both osteoblast differentiation models and OVX-induced osteoporosis models. We revealed that GPA strongly promotes bone formation by activating FXR/RUNX2 signaling. Moreover, the osteoporotic therapeutic effect of GPA was abolished in Fxr mice.

Conclusion: This study demonstrated that FXR is a promising target for treating osteoporosis and that GPA promotes bone formation in OVX-induced osteoporosis by activating FXR signaling. These findings provide novel insight into the mechanism by which GPA promotes bone formation and more evidence for its application in the treatment of osteoporosis.
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http://dx.doi.org/10.1016/j.phymed.2022.154258DOI Listing
August 2022

Thymol Increases Sensitivity of Clinical Col-R Gram-Negative Bacteria to Colistin.

Microbiol Spectr 2022 Jun 14:e0018422. Epub 2022 Jun 14.

Department of Medical Lab Science, School of Laboratory Medicine and Life Science, Wenzhou Medical Universitygrid.268099.c, Wenzhou, Zhejiang Province, China.

Colistin-resistant (Col-R) bacteria are increasing sharply, which poses a serious threat to public health. Thymol is a phenolic compound used for its wide-spectrum antimicrobial activity, while the combination of nontraditional drugs to restore colistin activity is an attractive strategy to treat infections caused by these pathogens. This study showed that thymol could play a synergistic role with colistin against Gram-negative bacteria (GNB), including nonfermenting bacteria and Enterobacteriaceae. According to antimicrobial resistance profiles, most of the colistin-resistant strains we collected showed multidrug-resistant (MDR) phenotypes. The checkerboard method and time-kill curve confirmed the synergistic effect of thymol combined with colistin against Col-R GNB. The synergistic antibiofilm activity of thymol combined with colistin was assessed via crystal violet staining and scanning electron microscopy (SEM) assays. Results showed that compared with a single drug, the combination partially destroyed bacterial cells and inhibit the formation of bacterial biofilms. Mechanismly, the thymol/colistin combination synergistically potentiated the antibacterial activity by accelerating the damage and permeability of the bacterial outer membrane. Preliminary data indicated that the thymol/colistin combination could decrease the number of bacteria ≥2 log CFU/mL after 24 h of therapy in a mouse thigh infection model. Our results fully prove that thymol and colistin combination possesses a promising treatment option against colistin-resistant GNB infections. Colistin is being considered "the last ditch" treatment in many infections caused by multidrug-resistant GNB clinical isolates, but colistin-resistant (Col-R) strains with different drug resistance mechanisms have appeared worldwide. Hence, it is of great significance to rejuvenate sensitization of clinical Col-R Gram-negative bacteria to colistin. In this study, the thymol/colistin combination showed notable antibacterial activity and . These findings suggest that the thymol/colistin combination may have promise as a treatment approach for treating the infections caused by Col-R pathogens.
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http://dx.doi.org/10.1128/spectrum.00184-22DOI Listing
June 2022

Liquid Biopsy Assessment of Circulating Tumor Cell PD-L1 and IRF-1 Expression in Patients with Advanced Solid Tumors Receiving Immune Checkpoint Inhibitor.

Target Oncol 2022 05 13;17(3):329-341. Epub 2022 Jun 13.

Clinical Research Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.

Background: Reliable biomarkers that can be serially monitored to predict treatment response to immune checkpoint inhibitors (ICIs) are still an unmet need. Here, we present a multiplex immunofluorescence (IF) assay that simultaneously detects circulating tumor cells (CTCs) and assesses CTC expression of programmed death ligand-1 (PD-L1) and interferon regulatory factor 1 (IRF-1) as a candidate biomarker related to ICI use.

Objective: To assess the potential of CTC PD-L1 and IRF-1 expression as candidate biomarkers for patients with advanced epithelial solid tumors receiving ICIs.

Patients And Methods: We tested the IF CTC assay in a pilot study of 28 patients with advanced solid tumors who were starting ICI. Blood for CTC evaluation was obtained prior to starting ICI, after a single cycle of therapy, and at the time of radiographic assessment or treatment discontinuation.

Results: At baseline, patients with 0-1 CTCs had longer progression-free survival (PFS) compared to patients with ≥ 2 CTCs (4.3 vs 1.3 months, p = 0.01). The presence of any PD-L1+ CTCs after a single dose of ICI portended shorter PFS compared to patients with no CTCs or PD-L1- CTCs (1.2 vs 4.2 months, p = 0.02); the presence of any PD-L1+ or IRF-1+ CTCs at time of imaging assessment or treatment discontinuation also was associated with shorter PFS (1.9 vs 5.5 months, p < 0.01; 1.6 vs 4.7 months, p = 0.05). CTC PD-L1 and IRF-1 expression did not correlate with tumor tissue PD-L1 or IRF-1 expression. Strong IRF-1 expression in tumor tissue was associated with durable (≥ 1 year) radiographic response (p = 0.02).

Conclusions: Based on these results, CTC PD-L1 and IRF-1 expression is of interest in identifying ICI resistance and warrants further study.
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http://dx.doi.org/10.1007/s11523-022-00891-0DOI Listing
May 2022

Deoxyschizandrin Inhibits the Proliferation, Migration, and Invasion of Bladder Cancer Cells through ALOX5 Regulating PI3K-AKT Signaling Pathway.

J Immunol Res 2022 25;2022:3079823. Epub 2022 May 25.

Department of Urology, The First Affiliated Hospital of Jiamusi University, Heilongjiang 154007, China.

Objective: Deoxyschizandrin has a significant inhibitory effect on a variety of tumor cells. However, the effect of Deoxyschizandrin on bladder cancer cells and its mechanism are still unclear.

Methods: Bladder cancer cells were treated with different concentrations of Deoxyschizandrin for 24 h, 48 h, and 72 h. The inhibition rate of cell proliferation was detected by CCK-8 assay. The changes of cell migration and invasion were detected by wound healing and Transwell assay. Based on the structure of Deoxyschizandrin, the protein targets of Deoxyschizandrin were predicted by bioinformatics database and verified by RNA and protein. Then, the expressions of ALOX5 and PI3K-AKT signaling pathway proteins were detected by Western blot in bladder cancer cells treated with Deoxyschizandrin.

Result: Deoxyschizandrin inhibited the proliferation, migration, and invasion of bladder cancer cells in a time- and concentration-dependent manner. Bioinformatics analysis showed that Deoxyschizandrin had 100 protein targets; among them, the score of ALOX5 was the highest, and the mRNA and protein levels of ALOX5 decreased after treatment with different concentrations of Deoxyschizandrin. Western blot results showed that compared with the control group, Deoxyschizandrin could significantly reduce the expression of p-PI3K and p-AKT, and overexpression of ALOX5 could significantly enhance the expression of p-PI3K and p-AKT. Compared with Deoxyschizandrin or overexpression of ALOX5, the expression of p-PI3K and p-AKT of Deoxyschizandrin combined with overexpression of ALOX5 recovered.

Conclusion: Deoxyschizandrin inhibits the proliferation, migration, and invasion of bladder cancer cells through ALOX5 regulating PI3K-AKT signaling pathway.
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http://dx.doi.org/10.1155/2022/3079823DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159825PMC
June 2022

Fabrication of Redox-Controllable Bioinspired Nanochannels for Precisely Regulating Protein Transport.

ACS Appl Mater Interfaces 2022 Jun 3. Epub 2022 Jun 3.

Key Laboratory of Pesticide and Chemical Biology (CCNU), Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China.

Redox regulation is an inherent feature of nature and plays a crucial role in the transport of ions/small molecules. However, whether redox status affects the biomolecule transport remains largely unknown. To explore the effects of redox status on biomolecule transport, herein, we constructed a glutathione/glutathione disulfide (GSH/GSSG)-driven and pillar[5]arene (P5)-modified artificial nanochannel for protein transport. The results indicate that hemoglobin (Hb) protein is selectively and effectively transported across the GSH-driven P5-modified nanochannel, which suggests that the redox status of the nanochannel could affect the process of protein transport. Therefore, this redox-driven nanochannel could provide a potential application for biomolecule detection and redox-controllable biomolecular drug release.
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http://dx.doi.org/10.1021/acsami.2c05594DOI Listing
June 2022

Regulation of endothelial nitric oxide synthase in cardiac remodeling.

Int J Cardiol 2022 Oct 30;364:96-101. Epub 2022 May 30.

Division of Cardiovascular Diseases, Department of Medicine(,) University of Tennessee Health Science Center, Memphis, TN, United States of America. Electronic address:

Objectives: Our previous study demonstrated that endothelial nitric oxide synthase (eNOS) gene serves as a candidate for modifiers of hypertrophic cardiomyopathy (HCM), which alters severity of HCM phenotypes. Herein, we sought to further elucidate the role of eNOS on cardiac myocyte hypertrophy and fibrosis, the major phenotypes of HCM.

Methods: Male eNOS-deficient mice (eNOS-/-) and wild type control mice (eNOS+/+, C57B1/6 J) were used in this study. Myocyte size was analyzed in hematoxylin/eosin stained sections using an image analyzing system. Cardiac β-myosin heavy chain (β-MHC) and α-skeletal actin (α-SKA) levels, markers of myocyte hypertrophy were evaluated by Western blot. Cardiac collagen volume fraction (CVF) was examined in picrosirius red stained section using an image analyzing system. Cardiac expression of tissue inhibitor of metalloproteinase 1 (TIMP-1) and transforming growth factor beta 1 (TGF-β1), markers of fibrosis, were determined by Western blot.

Results: Compared to eNOS+/+ mice, we found that; 1) myocyte size was significantly increased in eNOS-/- mice; 2) cardiac expression of β-MHC was markedly elevated, while α-SKA levels remained unchanged in eNOS-/- mice; 3) cardiac total and interstitial CVF levels were significantly higher in eNOS-/- mice; and 4) cardiac TIMP-1 levels were significantly greater in eNOS-/- mice, however, cardiac TGF-β1 was not differently expressed between the two groups.

Conclusion: The current study revealed that eNOS plays a beneficial role in cardiac remodeling, preventing the heart from development of myocyte hypertrophy and cardiac fibrosis. These findings support our previous report that eNOS may modify the severity of HCM phenotypes.
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http://dx.doi.org/10.1016/j.ijcard.2022.05.013DOI Listing
October 2022

miR-146a suppresses the expression of vascular endothelial growth factor and inflammatory responses in diabetic retinopathy.

Growth Factors 2022 May 23:1-9. Epub 2022 May 23.

Department of Endocrinology, The Fourth Hospital, Hebei Medical University, Shijiazhuang, Hebei, China.

This study was designed to explore the role of miR-146a in diabetic retinopathy (DR). 30 healthy control (HC), 50 patients with type 2 diabetes mellitus, and 48 DR patients were enrolled. Blood was collected and levels of miR-146a expression, vascular endothelial growth factor (VEGF), and three inflammatory cytokines (NF-κB, IL-1β, and TNF-α) were detected. Moreover, ARPE-19 cells were treated with miR-146a mimic or inhibitor in the presence of high glucose to evaluate its effect . DR patients had the lowest level of miR-146a and the highest level of VEGF as well as the most severe inflammation among the three groups. In addition, the miR-146a level was negatively correlated with the expression of VEGF and three inflammatory cytokines, respectively in DR patients. Moreover, VEGF expression was positively correlated with these three inflammatory cytokines in DR patients. In summary, miR-146a could inhibit VEGF expression and inflammation in DR.
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http://dx.doi.org/10.1080/08977194.2022.2077732DOI Listing
May 2022

Effect of phenyl numbers in polyphenyl ligand on retention properties of aromatic stationary phases.

J Chromatogr A 2022 Jul 14;1674:463152. Epub 2022 May 14.

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an 710127, China. Electronic address:

Aromatic phase, as one type of reversed-phase stationary phases, shows complementary selectivity to the n-alkyl counterparts especially for certain challenging separation tasks. However, effect of phenyl numbers in aromatic ligands on retention behaviors has rarely been addressed compared with the alkyl stationary phases. To illustrate the issue, a series of polyphenyl stationary phases were facially prepared via the coupling chemistry of isocyanate with amine, including aniline (π), 4-aminobiphenyl (π), 4-amino-p-terphenyl (π) and [1,1':4',1'':4'',1'''-quaterphenyl]-4-amine (π), respectively. The chromatographic behaviors of the new stationary phases as well as the traditional C18 were systematically compared in terms of retention mode, hydrophobic and aromatic selectivity, shape selectivity and π-π interaction by various analytes, including alkylbenzenes, polycyclic aromatic hydrocarbons congeners and substituted benzenes with electron-withdrawing groups. Due to the homologous structure of four polyphenyl ligands, the hydrophobic selectivity, aromatic selectivity and shape selectivity of stationary phases increase with phenyl numbers in the bonded polyphenyl ligands, whereas the increment becomes insignificant from U-π to U-π. This phenomenon is explained by the insertion degree of analytes in the polyphenyl ligand brushes. Compared with the homemade C18, the polyphenyl phases indicate insignificant changes of shape selectivity with temperature. Notably, the new polyphenyl phases demonstrate the great selective separation towards the electron-deficient compounds through the π-π interaction. These findings make up for the understanding of the retention behavior of aromatic stationary phases.
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http://dx.doi.org/10.1016/j.chroma.2022.463152DOI Listing
July 2022

Light-Induced Reversible Hierarchical Self-Assembly of Amphiphilic Diblock Copolymers into Microscopic Vesicles with Tunable Optical and Nanocarrier Properties.

ACS Macro Lett 2021 05 13;10(5):525-530. Epub 2021 Apr 13.

Shanghai Key Laboratory of Advanced Polymeric Materials, Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.

In contrast to the conventional hierarchical self-assembly process, effective methods to enable reversible hierarchical self-assembly of block copolymers are comparatively few and limited in scope. Herein, we report, for the first time, a simple yet robust strategy for light-induced reversible hierarchical self-assembly of an amphiphilic diblock copolymer, poly(4-vinylpyridine)--poly[6-[4-(4-butyloxyphenylazo)phenoxy]hexyl methacrylate] (denoted P4VP--PAzoMA). The hierarchical structures are constructed via a two-step self-assembly process (first-level reverse micelles, second-level compound micelles, and rearrangement into micrometer-sized vesicles) driven by use of solvent. Intriguingly, because of reversible photoinduced -to- isomerization of azobenzene moieties in PAzoMA, the vesicles could disassemble into subunits upon UV light and then recover the nearly identical vesicular morphology upon visible light. Such a reversible hierarchical self-assembly process is accompanied by reversible fluorescence, encapsulation, and controlled release of dyes and can be used as a template for the synthesis of nanoparticles. Clearly, the ability to render the light-enabled reversible hierarchical self-assembly provides a unique platform for smart delivery vehicles and templates for nanomaterials.
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http://dx.doi.org/10.1021/acsmacrolett.1c00127DOI Listing
May 2021

Predicting clinical outcomes using artificial intelligence and machine learning in neonatal intensive care units: a systematic review.

J Perinatol 2022 May 13. Epub 2022 May 13.

Child Health Imprints (CHIL) USA Inc, Madison, WI, USA.

Background: Advances in technology, data availability, and analytics have helped improve quality of care in the neonatal intensive care unit.

Objective: To provide an in-depth review of artificial intelligence (AI) and machine learning techniques being utilized to predict neonatal outcomes.

Methods: The PRISMA protocol was followed that considered articles from established digital repositories. Included articles were categorized based on predictions of: (a) major neonatal morbidities such as sepsis, bronchopulmonary dysplasia, intraventricular hemorrhage, necrotizing enterocolitis, and retinopathy of prematurity; (b) mortality; and (c) length of stay.

Results: A total of 366 studies were considered; 68 studies were eligible for inclusion in the review. The current set of predictor models are primarily built on supervised learning and mostly used regression models built on retrospective data.

Conclusion: With the availability of EMR data and data-sharing of NICU outcomes across neonatal research networks, machine learning algorithms have shown breakthrough performance in predicting neonatal disease.
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http://dx.doi.org/10.1038/s41372-022-01392-8DOI Listing
May 2022

NIR-II Emissive Ru(II) Metallacycle Assisting Fluorescence Imaging and Cancer Therapy.

Small 2022 06 12;18(23):e2201625. Epub 2022 May 12.

Key Laboratory of Pesticides and Chemical Biology (Ministry of Education), College of Chemistryk, Central China Normal University, Wuhan, 430079, China.

Despite the success of emissive Ruthenium (Ru) agents in biomedicine, problems such as the visible-light excitation/emission and single chemo- or phototherapy modality still hamper their applications in deep-tissue imaging and efficient cancer therapy. Herein, an second nearinfrared window (NIR-II) emissive Ru(II) metallacycle (Ru1000, λ  = 1000 nm) via coordination-driven self-assembly is reported, which holds remarkable deep-tissue imaging capability (≈6 mm) and satisfactory chemo-phototherapeutic performance. In vitro results indicate Ru1000 displays promising cellular uptake, good cancer-cell selectivity, attractive anti-metastasis properties, and remarkable anticancer activity against various cancer cells, including cisplatin-resistant A549 cells (IC  = 3.4 × 10  m vs 92.8 × 10  m for cisplatin). The antitumor mechanism could be attributed to Ru1000-induced lysosomal membrane damage and mitochondrial-mediated apoptotic cell death. Furthermore, Ru1000 also allows the high-performance in vivo NIR-II fluorescence imaging-guided chemo-phototherapy against A549 tumors. This work may provide a paradigm for the development of long-wavelength emissive metallacycle-based agents for future biomedicine.
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http://dx.doi.org/10.1002/smll.202201625DOI Listing
June 2022

Acquisition of Daptomycin Resistance by Confers Collateral Sensitivity to Glycopeptides.

Front Microbiol 2022 12;13:815600. Epub 2022 Apr 12.

Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Daptomycin is a last-line antibiotic used in the treatment of multidrug-resistant infections. Alarmingly, daptomycin-resistant isolates have emerged. In this study, we investigated the evolution and mechanisms of daptomycin resistance in clinical isolates and the corresponding acquisition of collateral sensitivity (CS) as an evolutionary trade-off. We evolved daptomycin resistance in six daptomycin-susceptible isolates to obtain daptomycin-resistant mutants. The six strains successfully acquired high-level resistance to daptomycin , but this led to fitness costs in terms of growth, competition, and virulence. Mutations in , , and ; increased surface positive charge; thicker cell walls; and elevated expression of and were observed in daptomycin-resistant mutants. Surprisingly, we observed the emergence of CS in SC1762 isolates after the induction of daptomycin resistance. Compared with parental strains, the SC1174-D strain (i.e., daptomycin-resistant mutant of SC1174; non-CS) showed significantly upregulated expression of the gene cluster. However, in SC1762-D (i.e., daptomycin-resistant mutant of SC1762), all cluster genes except the gene were obviously downregulated. Further analyses revealed that an ISbased composite transposon was generated in SC1762-D, and it disrupted the gene, likely affecting the structure and expression of the gene cluster and resulting in resensitization to glycopeptides. Overall, this study reports a novel form of CS between daptomycin and glycopeptides in . Further, it provides a valuable foundation for developing effective regimens and sequential combinations of daptomycin and glycopeptides against .
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http://dx.doi.org/10.3389/fmicb.2022.815600DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9041417PMC
April 2022

On-site, rapid and visual method for nanomolar Hg detection based on the thymine-Hg-thymine triggered "double" aggregation of Au nanoparticles enhancing the Tyndall effect.

RSC Adv 2021 Nov 17;11(58):36859-36865. Epub 2021 Nov 17.

Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology Guilin 541004 P. R. China +86 773 5896839 +86 773 5896453.

This work describes a new nanosensor for the simple, rapid, portable, colorimetric analysis of mercury(ii) (Hg) ions by combining the sensitive Tyndall effect (TE) of colloidal Au nanoparticles (AuNPs) with specific thymine-Hg-thymine (T-Hg-T) coordination chemistry for the first time. For the TE-inspired assay (TEA), in the presence of Hg in a sample, the analyte can selectively mediate the hybridization of three types of flexible single-stranded DNAs (ssDNAs) to form stable rigid double-stranded DNAs (dsDNAs) the T-Hg-T ligand interaction. Subsequent self-assembly of the dsDNAs with terminal thiol groups on the AuNPs' surfaces led to their "double" aggregation in addition to the lack of sufficient ssDNAs as the stabilizing molecules in a high-salt solution, resulting in a remarkably enhanced TE signal that positively relied on the Hg level. The results demonstrated that such a TEA method enabled rapid naked-eye qualitative analysis of 625 nM Hg within 10 min with an inexpensive laser pointer pen as an inexpensive handheld light source to generate the TE response. Making use of a smartphone for portable TE readout could further quantitatively detect the Hg ions in a linear concentration range from 156 to 2500 nM with a limit of detection as low as 25 nM. Moreover, the developed equipment-free nanosensor was also used to analyze the Hg ions in real samples including tap water, drinking water, and pond water, the obtained recoveries were within the range of 93.68 to 108.71%. To the best of our knowledge, this is the first report of using the AuNPs and functional nucleic acids to design a TE-based biosensor for the analysis of highly toxic heavy metal ions.
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http://dx.doi.org/10.1039/d1ra07211kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043572PMC
November 2021

A novel strategy for sensitive and rapid detection of ascorbic acid the Tyndall effect of cobalt hydroxide nanoflakes.

RSC Adv 2021 Dec 8;11(62):39306-39310. Epub 2021 Dec 8.

College of Chemistry and Bioengineering, Guilin University of Technology Guilin 541004 P. R. China +86 773 5896839 +86 773 5896453.

Cobalt oxyhydroxide (CoOOH) nanoflakes, as nanoenzymes and fluorescence quenchers, have been widely used in colorimetric and fluorescent analysis. However, their promising light scattering property-the Tyndall effect (TE)-has never been applied in biosensors and biological analysis to date. Herein, we report for the first time a novel strategy for point-of-care detection of ascorbic acid (AA) with the TE of CoOOH nanoflakes providing colorimetric signaling. In this detection system, CoOOH nanoflakes exhibit a strong red TE signal under the illumination of a hand-held 635 nm laser pointer pen. However, the introduction of AA could induce a significant decrease of the TE because it could reduce CoOOH into Co and results in the degradation of the CoOOH nanoflakes. The changes in the TE intensity could be read-out using a smartphone for the portable quantitative analysis of AA. The results showed that this CoOOH nanoflake-based TE-inspired assay (TEA) exhibited a good linear range from 0.25 μM to 40 μM for AA, with a detection limit of 12 nM. It also showed high selectivity toward AA over common potential interfering species. Importantly, this method possessed the advantages of simple operation, low consumption of time and equipment-free analysis and was successfully applied to the detection of AA in vitamin C tablets.
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http://dx.doi.org/10.1039/d1ra07702cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044414PMC
December 2021

Being Hopeful and Mindful During Adversity: A Longitudinal Study on College Students' Adjustment During COVID-19.

Mindfulness (N Y) 2022 19;13(6):1499-1509. Epub 2022 Apr 19.

Centre for Child and Family Science, The Education University of Hong Kong, Taipo, Hong Kong.

Objectives: The current study examined whether hope and mindfulness were associated with changes in two maladjustment measures, internalizing and externalizing behaviors, during the COVID-19 pandemic and tested sense of coherence as a mediator. The salutogenic theory of health, which posits that sense of coherence is central to individuals' well-being in stressful situations and that individuals derive their sense of coherence from their generalized resistance resources (GRRs), was used to guide the analyses.

Methods: On two occasions separated by about 6 months, 253 Hong Kong college students (mean age = 21.0 years at time 1; 86% of them were women) filled in online questionnaires during the COVID-19 outbreaks. Path analysis was conducted to examine the interrelationships among hope and mindfulness, sense of coherence, and internalizing and externalizing behaviors.

Results: Results indicated that hope and mindfulness at time 1 were associated with internalizing and externalizing behaviors at time 2, even after controlling for confounding variables and prior levels of internalizing and externalizing behaviors. Moreover, sense of coherence at time 1 significantly mediated these associations.

Conclusions: Findings pointed to the potential roles of hope, mindfulness, and sense of coherence in understanding Chinese college students' adjustment during the COVID-19 pandemic. Future research is needed to test whether sense of coherence and behavioral adjustment can be promoted through hope- and mindfulness-based intervention programs. Future research is also needed to examine the interrelationships among health-promoting assets, sense of coherence, and individual adjustment in samples of diverse cultural backgrounds.
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http://dx.doi.org/10.1007/s12671-022-01892-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016695PMC
April 2022

Construction of emissive ruthenium(II) metallacycle over 1000 nm wavelength for in vivo biomedical applications.

Nat Commun 2022 04 14;13(1):2009. Epub 2022 Apr 14.

Key Laboratory of Pesticides and Chemical Biology, Ministry of Education, International Joint Research Center for Intelligent Biosensor Technology and Health, College of Chemistry, Central China Normal University, Wuhan, Hubei, 430079, China.

Although Ru(II)-based agents are expected to be promising candidates for substituting Pt-drug, their in vivo biomedical applications are still limited by the short excitation/emission wavelengths and unsatisfactory therapeutic efficiency. Herein, we rationally design a Ru(II) metallacycle with excitation at 808 nm and emission over 1000 nm, namely Ru1085, which holds deep optical penetration (up to 6 mm) and enhanced chemo-phototherapy activity. In vitro studies indicate that Ru1085 exhibits prominent cell uptake and desirable anticancer capability against various cancer cell lines, especially for cisplatin-resistant A549 cells. Further studies reveal Ru1085 induces mitochondria-mediated apoptosis along with S and G2/M phase cell cycle arrest. Finally, Ru1085 shows precise NIR-II fluorescence imaging guided and long-term monitored chemo-phototherapy against A549 tumor with minimal side effects. We envision that the design of long-wavelength emissive metallacycle will offer emerging opportunities of metal-based agents for in vivo biomedical applications.
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http://dx.doi.org/10.1038/s41467-022-29572-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010459PMC
April 2022

N-type calcium channel and renal injury.

Int Urol Nephrol 2022 Apr 13. Epub 2022 Apr 13.

Department of Pharmacology, Kagawa University Medical School, Kagawa, 761-0793, Japan.

Accumulating evidences indicated that voltage-gated calcium channels (VDCC), including L-, T-, N-, and P/Q-type, are present in kidney and contribute to renal injury during various chronic diseases trough different mechanisms. As a voltage-gated calcium channel, N-type calcium channel was firstly been founded predominately distributed on nerve endings which control neurotransmitter releases. Since sympathetic nerve is distributed along renal afferent and efferent arterioles, N-type calcium channel blockade on sympathetic nerve terminals would bring renal dynamic improvement by dilating both arterioles and reducing glomerular pressure. In addition, large body of scientific research indicated that neurotransmitters, such as norepinephrine, releases by activating N-type calcium channel can trigger inflammatory and fibrotic signaling pathways in kidney. Interestingly, we recently demonstrated that N-type calcium channel is also expressed on podocytes and may directly contribute to podocyte injury in denervated animal models. In this paper, we will summarize our current knowledge regarding renal N-type calcium channels, and discuss how they might contribute to the river that terminates in renal injury.
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http://dx.doi.org/10.1007/s11255-022-03183-8DOI Listing
April 2022

Five weeks of Yuishinkai karate training improves balance and neuromuscular function in older adults: a preliminary study.

BMC Sports Sci Med Rehabil 2022 Apr 11;14(1):65. Epub 2022 Apr 11.

Rehabilitation Neuroscience Laboratory, University of Victoria, Room 172, McKinnon Building, 3800 Finnerty Road, Victoria, BC, V8P 5C2, Canada.

Background: Martial arts training has shown positive impacts on balance and physiological measurements. Further investigation of the contents and feasibility of an effective therapeutic assessment of martial arts is needed in older adults, mainly for future applications and real-world implementation.

Methods: Sixteen older adults (8 male, 8 female, age 59-90 years), with or without chronic conditions, participated in a preliminary study using 5-weeks of karate training and a triple baseline control procedure. Group and single subject data analyses were conducted for dynamic balance, Timed Up and Go (TUG), hand grip, ankle plantarflexion force, and spinal cord excitability (via the soleus H-reflex) pre- and post-training.

Results: On average, participants completed a total of 2437 steps, 1762 turns, 3585 stance changes, 2047 punches, 2757 blocks, and 1253 strikes. Karate training improved dynamic balance performance such that the group average time was reduced (time to target (-13.6%, p = 0.020) and time to center (-8.3%, p = 0.010)). TUG was unchanged when considering the entire group (p = 0.779), but six participants displayed significant changes. Left handgrip (7.9%, p = 0.037), and plantarflexion force in the right (28.8%, p = 0.045) and left leg (13.3%, p = 0.024) increased for the group. Spinal cord excitability remained unchanged in group data analysis but 5 individuals had modulated H/M ratios.

Conclusion: 5-weeks of karate training delivered in a fashion to mimic generally accessible community-level programs improved balance and strength in older adults. Whole-body movement embodied in karate training enhanced neuromuscular function and postural control. We met the overriding goal of this preliminary study to emphasize and assess feasibility and safety for the generalizability of martial arts interventions to real-world communities to impact health outcomes. Further quantitative work should explore threshold dose and development of martial arts training interventions as potential "exercise is medicine" functional fitness for older adults.
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http://dx.doi.org/10.1186/s13102-022-00458-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997192PMC
April 2022

Upconversion nanoparticles regulated drug & gas dual-effective nanoplatform for the targeting cooperated therapy of thrombus and anticoagulation.

Bioact Mater 2022 Dec 17;18:91-103. Epub 2022 Mar 17.

The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi, 330088, PR China.

Thromboembolism is the leading cause of cardiovascular mortality. Currently, for the lack of targeting, short half-life, low bioavailability and high bleeding risk of the classical thrombolytic drugs, pharmacological thrombolysis is usually a slow process based on micro-pumping. In addition, frequently monitoring and regulating coagulation functions are also required during (and after) the process of thrombolysis. To address these issues, a targeted thrombolytic and anticoagulation nanoplatform (UCATS-UK) is developed based on upconversion nanoparticles (UCNPs) that can convert 808 or 980 nm near-infrared (NIR) light into UV/blue light. This nanoplatform can target and enrich in the thrombus site. Synergistic thrombolysis and anticoagulation therapy thus could be realized through the controlled release of urokinase (UK) and nitric oxide (NO). Both and experiments have confirmed the excellent thrombolytic and anticoagulative capabilities of this multifunctional nanoplatform. Combined with the unique fluorescent imaging capability of UCNPs, this work is expected to contribute to the development of clinical thrombolysis therapy towards an integrated system of imaging, diagnosis and treatment.
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http://dx.doi.org/10.1016/j.bioactmat.2022.03.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961464PMC
December 2022

Editorial: Precise Diagnosis and Therapy Using Near-Infrared Light.

Front Bioeng Biotechnol 2022 1;10:864759. Epub 2022 Mar 1.

Department of Chemical Biology, Centre Normal University, Wuhan, China.

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http://dx.doi.org/10.3389/fbioe.2022.864759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8921661PMC
March 2022

1894 revisited: Cross-education of skilled muscular control in women and the importance of representation.

PLoS One 2022 17;17(3):e0264686. Epub 2022 Mar 17.

Rehabilitation Neuroscience Laboratory, University of Victoria, Victoria, British Columbia, Canada.

In 1894 foundational work showed that training one limb for "muscular power" (i.e. strength) or "muscular control" (i.e. skill) improves performance in both limbs. Despite that the original data were exclusively from two female participants ("Miss Smith" and "Miss Brown"), in the decades that followed, such "cross-education" training interventions have focused predominantly on improving strength in men. Here, in a female cohort, we revisit that early research to underscore that training a task that requires precise movements in a timely fashion (i.e. "muscular control") on one side of the body is transferred to the contralateral untrained limb. With unilateral practice, women reduced time to completion and the number of errors committed during the commercially available game of Operation® Iron Man 2 with both limbs. Modest reductions in bilateral Hoffmann (H-) reflex excitability evoked in the wrist flexors suggest that alterations in the spinal cord circuitry may be related to improvements in performance of a fine motor task. These findings provide a long overdue follow-up to the efforts of Miss Theodate L. Smith from more than 125 years ago, highlight the need to focus on female participants, and advocate more study of cross-education of skilled tasks.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0264686PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8929574PMC
April 2022
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