Publications by authors named "Yao Ma"

194 Publications

CBS-derived H2S facilitates host colonization of Vibrio cholerae by promoting the iron-dependent catalase activity of KatB.

PLoS Pathog 2021 Jul 20;17(7):e1009763. Epub 2021 Jul 20.

Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.

Sensing and resisting oxidative stress is critical for Vibrio cholerae to survive in either the aquatic environment or the gastrointestinal tract. Previous studies mainly focused on the mechanisms of oxidative stress response regulation that rely on enzymatic antioxidant systems, while functions of non-enzymatic antioxidants are rarely discussed in V. cholerae. For the first time, we investigated the role of hydrogen sulfide (H2S), the simplest thiol compound, in protecting V. cholerae against oxidative stress. We found that degradation of L-cysteine by putative cystathionine β-synthase (CBS) is the major source of endogenous H2S in V. cholerae. Our results indicate that intracellular H2S level has a positive correlation with cbs expression, while the enhanced H2S production can render V. cholerae cells less susceptible to H2O2 in vitro. Using proteome analysis and real-time qPCR assay, we found that cbs expression could stimulate the expression of several enzymatic antioxidants, including reactive oxygen species (ROS) detoxifying enzymes SodB, KatG and AhpC, the DNA protective protein DPS and the protein redox regulator Trx1. Assays of ROS detoxification capacities revealed that CBS-derived H2S could promote catalase activity at the post-translational level, especially for KatB, which serves as an important way that endogenous H2S participates in H2O2 detoxification. The enhancement of catalase activity by H2S is achieved through facilitating the uptake of iron. Adult mice experiments showed that cbs mutant has colonization defect, while either complementation of cbs or exogenous supplement of N-Acetyl-L-Cysteine restores its fitness in the host environment. Herein, we proposed that V. cholerae regulates CBS-dependent H2S production for better survival and proliferation under ROS stress.
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http://dx.doi.org/10.1371/journal.ppat.1009763DOI Listing
July 2021

[Annual review of capillary electrophoresis technology in 2020].

Se Pu 2021 Jun;39(6):559-566

School of Life Science, Beijing Institute of Technology, Beijing 100081, China.

This paper reviews the developments in capillary electrophoresis (CE) in 2020. A total of 222 research papers related to CE technology published in 2020 were retrieved from the ISI Web of Science. These papers were selected by using the keywords "capillary electrophoresis-mass spectrometry", "capillary isoelectric focusing", "micellar electrokinetic chromatography", or "capillary electrophoresis" as search conditions. A further 37 research papers related to CE technology were found in Chinese journals: and . The 38 papers published in 13 different journals with an impact factor (IF)≥5.0 are individually discussed. In particular, the journal Analytical Chemistry (IF=6.8) was found to have nine relevant articles, eight of which were related to CE-MS technology and its application, while (IF=6.3) published five articles with four papers based on micellar electrokinetic chromatography (MEKC) and its combined technology. This demonstrated that MEKC can simultaneously separate negatively charged, positively charged, and neutral particles, in addition to featuring the advantage of combining different detector applications for the analysis of target matter in a complex matrix. In (IF=6.0), five articles were collected, which mainly focused on new applications of CE-MS and multimode CE research. In (IF=5.3), 10 articles were collected, which focused on new methods and applications of CE and CE-MS. The remaining nine articles, i. e., seven experimental articles, one review article, and one introduction of scientific research achievements, were published in various other high-impact journals. At the same time, 184 articles were found in 89 journals with IF<5.0. In particular, the (IF=4.1) and (IF=3.1) contained intensive reports for CE: 16 and 24 publications in relevant areas. The representative contents of 40 articles published in two journals were introduced comprehensively in this review, including new materials and new applications of MEKC, as well as new applications of CE-MS and CE. An overview of the work in the 37 articles collected from Chinese journals included "Aptamer Special Issue" published by and two CE technical special issues published by . In the "Aptamer Special Issue", Qu Feng's research group from Beijing Institute of Technology reported five works on aptamers based on CE, while the contents of include new applications of CE and new materials in capillary tubes. In summary, new methods and applications of capillary electrophoresis-mass spectrometry (CE-MS) were the prominent development trend in CE technology in the year 2020, with the main focus being on the combination of CE-MS with electrochemical detection, solid-phase extraction, and various capillary electrophoresis modes. The number of reports related to the CE-MS interface has decreased compared to that in previous years. Conventional CE technology is mainly focused on the applications of MEKC in the analysis and concentration of complex samples, especially for the analysis of complex matrix samples such as food and drugs. In addition, the scientific research achievements of Chinese experts and scholars in CE-related fields cover the application of CE in life sciences, clinical medicine, pharmaceutical research and development, environmental science, natural products, food analysis, etc., representing the level and current status of the application of CE research in China.
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http://dx.doi.org/10.3724/SP.J.1123.2021.03024DOI Listing
June 2021

[Annual review of capillary electrophoresis technology in 2019].

Se Pu 2020 Sep;38(9):986-992

School of Life Science, Beijing Institute of Technology, Beijing 100081, China.

This paper reviews the capillary electrophoresis (CE) in 2019. The literatures searched from ISI Web of Science published in 2019 (Jan.1st to Dec.31th) are classified and introduced based on bioanalysis, drug analysis, clinical tests and medical diagnosis, chiral separation, foods tests, other compounds and ion analysis, and the application of capillary electrophoresis-mass spectrometry (CE-MS). International and domestic conferences related to CE and the important research reports are briefly introduced.
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http://dx.doi.org/10.3724/SP.J.1123.2020.03027DOI Listing
September 2020

Modeling the emissions of rural vehicles based on real-world driving cycles.

Sci Total Environ 2021 Jun 16;793:148380. Epub 2021 Jun 16.

State Environmental Protection Key Laboratory of Vehicle Emission Control and Simulation, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; Vehicle Emission Control Center of Ministry of Ecology and Environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.

Characterized by a high frequency of use, harsh working environments, poor maintenance, and low levels of emission controls, rural vehicles (RVs) are becoming an important source of air pollution. Our study used a portable emission measurement system (PEMS) to test the real-world emissions of 35 RVs on provincial, rural, and farm roads. The results show that high emission rates of carbon monoxide (CO) and hydrocarbons (HC) mainly occurred when accelerating at low speeds. However, high levels of nitrogen oxides (NO) were emitted during high-speed acceleration. The particulate number (PN) of emissions was higher when the RVs were accelerating. According to the overall test results, the vehicle specific power (VSP) on the provincial road mostly ranged within (0, 3], accounting for 68.80% of the total. The VSP on rural and farm roads was concentrated within (0, 2] kW·ton, accounting for 67.09% and 76.64% of the total, respectively. We defined 14 bins based on the distribution of the VSP values and calculated the average emission rate of each bin. By comparing the average emission rate among the bins, we found that within Bins 1-7 (VSP < 0 kW·ton), CO, HC, and NO emissions slowly increased as the VSP increased. In Bins 8-13 (VSP ≥ 0 kW·ton), the average emission rates of four pollutants increased as the VSP increased. However, all pollutants decreased in Bin 14 (VSP ≥ 6 kW·ton). We built a microscopic emission model according to the VSP distribution characteristics of RVs on different road types. We compared the measured and simulated emission factors and found that our emission model can greatly simulate the HC, NOx and PN emission factors of RVs.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148380DOI Listing
June 2021

Skin vaccination with dissolvable microneedle patches incorporating influenza neuraminidase and flagellin protein nanoparticles induces broad immune protection against multiple influenza viruses.

ACS Appl Bio Mater 2021 Jun 11;4(6):4953-4961. Epub 2021 May 11.

Center for Inflammation, Immunity & Infection, Georgia State University Institute for Biomedical Sciences, 100 Piedmont Road, Atlanta, Georgia 30302, USA.

We generated self-adjuvanted protein nanoparticles of conserved influenza antigens and immunized mice via skin vaccination with dissolvable microneedle patches (MNPs) to increase the strength and breadth of immune responses. We produced M2e nanoparticles via ethanol desolvation, and double-layered NA1/M2e (shell/core), NA1-FliC/M2e, NA2/M2e, and NA2-FliC/M2e protein nanoparticles by chemically crosslinking influenza NA and flagellin (FliC) onto the surfaces of the M2e nanoparticles. The resulting nanoparticles retained FliC TLR5 innate signaling activity and significantly increased antigen-uptake and dendritic cell maturation . We incorporated the nanoparticles into MNPs for skin vaccination in mice. The nanoparticle MNPs significantly increased M2e and NA-specific antibody levels, the numbers of germinal center B cells, and IL-4 positive splenocytes. Double-layered nanoparticle MNP skin vaccination protected mice against homologous and heterosubtypic influenza viruses. Our results demonstrated that MNP skin vaccination of NA-FliC/M2e nanoparticles could be developed into a standalone or synergistic component of a universal influenza vaccine strategy.
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http://dx.doi.org/10.1021/acsabm.1c00240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232372PMC
June 2021

Abnormal Liver Function Tests Were Associated With Adverse Clinical Outcomes: An Observational Cohort Study of 2,912 Patients With COVID-19.

Front Med (Lausanne) 2021 9;8:639855. Epub 2021 Jun 9.

State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China.

The impact of liver function test (LFTs) abnormality on adverse clinical outcomes in coronavirus disease 2019 (COVID-19) patients remains controversial. The aim of this study was to assess the impact of abnormal LFTs on clinical outcomes in a large cohort of hospitalized patients with COVID-19. We retrospectively collected data on 2,912 consecutive patients with COVID-19 who were admitted to a makeshift hospital in China between 5 February and 23 March 2020. The association between LFTs abnormalities (baseline and peak values) and clinical outcomes was measured by using Cox regression models. On admission 1,414 patients (48.6%) had abnormal LFTs, with alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), alkaline phosphatase (ALP), and gamma-glutamyltransferase (GGT) elevation in 662 (22.7%), 221 (7.6%), 52 (1.8%), 135 (4.6%), and 536 (18.5%) patients, respectively, and hypoalbuminemia in 737 (25.3%) patients. During a median 13 (IQR: 8-19) days of hospitalization, 61 patients (2.1%) died, 106 patients (3.6%) admitted to intensive care unit (ICU), and 75 patients (2.6%) required mechanical ventilation. After adjustment for confounders, baseline abnormal LFTs were independently associated with increased risks of mortality (adjusted HR 3.66, 95%CI 1.64-8.19, = 0.002), ICU admission (adjusted HR 3.12 95%CI 1.86-5.23, < 0.001), and mechanical ventilation (adjusted HR 3.00, 95%CI 1.63-5.52, < 0.001), which was homogeneous across the severity of COVID-19 infection. Among the parameters of LTFs, the associations with the outcomes were more pronounced for AST and albumin abnormality. In contrast, ALT elevation was not significantly associated with those outcomes. Similar results were observed for peak values of LFTs during hospitalization. Abnormality of AST, albumin, TBIL, ALP, and GGT but not ALT were independently associated with adverse outcomes.
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http://dx.doi.org/10.3389/fmed.2021.639855DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219933PMC
June 2021

Surface illuminated interdigitated Ge-on-Si photodetector with high responsivity.

Opt Express 2021 May;29(11):16346-16361

To address the problem of traditional surface illuminated detectors being of low responsivity, this work proposes a large-size interdigitated "finger-type" germanium-on-silicon (Ge-on-Si) photodetector (PD) based on the surface illumination approach. For 1550 nm light with a surface incident power of -20 dBm at room temperature, the best responsivity of the PD achieved is ∼0.64 A/W at 0.5 V. At the same time, the optimal bandwidth reaches 1.537 MHz with 3.5 V applied voltage. In order to suppress the dark current induced noise, a Ge-on-Si avalanche photodiode (APD) with the interdigitated structure is designed. The avalanche voltage is designed ∼13.3 V at room temperature, and the dark current density in linear region is at mA/cm order. We believe this type of device can be applied in weak light detection condition.
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http://dx.doi.org/10.1364/OE.427343DOI Listing
May 2021

The role of SARC-F scale in predicting progression risk of COVID-19 in elderly patients: a prospective cohort study in Wuhan.

BMC Geriatr 2021 06 10;21(1):355. Epub 2021 Jun 10.

Global Health Research Center, Duke Kunshan University, Suzhou, Jiangsu Province, China.

Background: Since the outbreak of COVID-19, it has been documented that old age and underlying illnesses are associated with poor prognosis among COVID-19 patients. However, it is unknown whether sarcopenia, a common geriatric syndrome, is associated with poor prognosis among older COVID-19 patients. The aim of our prospective cohort study is to investigate the association between sarcopenia risk and severe disease among COVID-19 patients aged ≥60 years.

Method: A prospective cohort study of 114 hospitalized older patients (≥60 years) with confirmed COVID-19 pneumonia between 7 February, 2020 and 6 April, 2020. Epidemiological, socio-demographic, clinical and laboratory data on admission and outcome data were extracted from electronic medical records. All patients were assessed for sarcopenia on admission using the SARC-F scale and the outcome was the development of the severe disease within 60 days. We used the Cox proportional hazards model to identify the association between sarcopenia and progression of disease defined as severe cases in a total of 2908 person-days.

Result: Of 114 patients (mean age 69.52 ± 7.25 years, 50% woman), 38 (33%) had a high risk of sarcopenia while 76 (67%) did not. We found that 43 (38%) patients progressed to severe cases. COVID-19 patients with higher risk sarcopenia were more likely to develop severe disease than those without (68% versus 22%, p < 0.001). After adjustment for demographic and clinical factors, higher risk sarcopenia was associated with a higher hazard of severe condition [hazard ratio = 2.87 (95% CI, 1.33-6.16)].

Conclusion: We found that COVID-19 patients with higher sarcopenia risk were more likely to develop severe condition. A clinician-friendly assessment of sarcopenia could help in early warning of older patients at high-risk with severe COVID-19 pneumonia.
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http://dx.doi.org/10.1186/s12877-021-02310-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190739PMC
June 2021

Straw Incorporation with Nitrogen Amendment Shapes Bacterial Community Structure in an Iron-Rich Paddy Soil by Altering Nitrogen Reserves.

Microorganisms 2021 May 3;9(5). Epub 2021 May 3.

College of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, China.

Incorporation of crop straw into the soil along with inorganic fertilization is a widespread agricultural practice and is essential in nutrient-scarce soils, such as iron-rich (ferruginous) paddy soils. The responses of soil bacterial communities to straw incorporation under different nitrogen inputs in iron-rich soils remain unclear. Therefore, 6000 kg ha dry wheat ( L. cv. Zhengmai 12) straw was applied to a rice paddy with and without nitrogen amendment (0, 80, 300, and 450 kg ha N as urea), to investigate its effects on soil fertility and bacterial community structure. Organic matter, total nitrogen, and water contents tended to decrease in straw-incorporated soils with different nitrogen inputs. Proteobacteria was the dominant bacterial phylum across all treatments (26.3-32.5% of total sequences), followed by Chloroflexi, Acidobacteria, and Nitrospirae. Up to 18.0% of all the taxa in the bacterial communities were associated with iron cycling. Straw incorporation with nitrogen amendment increased the relative abundance of iron oxidizers, Gallionellaceae, while decreasing the relative abundance of iron reducers, Geobacteraceae. Bacterial community composition shifted in different treatments, with total nitrogen, water, and Fe(III) contents being the key drivers. Straw incorporation supplemented by 300 kg ha N increased bacterial richness and enhanced all the predicted bacterial functions, so that it is recommended as the optimal nitrogen dosage in practice.
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http://dx.doi.org/10.3390/microorganisms9050988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147819PMC
May 2021

Therapeutic Effects of Naringin in Rheumatoid Arthritis: Network Pharmacology and Experimental Validation.

Front Pharmacol 2021 14;12:672054. Epub 2021 May 14.

Department of Joint Surgery, Xi'an Jiaotong University Affiliated HongHui Hospital, Xi'an, China.

Rheumatoid arthritis is a chronic autoimmune disease characterized by persistent hyperplasia of the synovial membrane and progressive erosion of articular cartilage. Disequilibrium between the proliferation and death of RA fibroblast-like synoviocytes (RA-FLSs) is the critical factor in progression of RA. Naringin has been reported to exert anti-inflammatory and antioxidant effect in acute and chronic animal models of RA. However, the therapeutic effect and underlying mechanisms of naringin in human RA-FLS remain unclear. Based on network pharmacology, the corresponding targets of naringin were identified using SwissTargetPrediction database, STITCH database, and Comparative Toxicogenomics Database. Deferentially expressed genes (DEGs) in RA were obtained from the GEO database. The protein-protein interaction (PPI) networks of intersected targets were constructed using the STRING database and visualized using Cytoscape. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed, and the pathways directly related to pathogenesis of RA were integrated manually. Further, studies were carried out based on network pharmacology. 99 target genes were intersected between targets of naringin and DEGs. The PPI network and topological analysis indicated that IL-6, MAPK8, MMP-9, TNF, and MAPK1 shared the highest centrality among all. GO analysis and KEGG analysis indicated that target genes were mostly enriched in (hsa05200) pathways in cancer, (hsa05161) hepatitis B, (hsa04380) osteoclast differentiation, (hsa04151) PI3K-Akt signaling pathway, and (hsa05142) Chagas disease (American trypanosomiasis). studies revealed that naringin exposure was found to promote apoptosis of RA-FLS, increased the activation of caspase-3, and increased the ratio of Bax/Bcl-2 in a dose-dependent manner. Furthermore, treatment of naringin attenuated the production of inflammatory cytokines and matrix metalloproteinases (MMPs) in TNF-ɑ-induced RA-FLS. Moreover, treatment of naringin inhibited the phosphorylation of Akt and ERK in RA-FLS. Network pharmacology provides a predicative strategy to investigate the therapeutic effects and mechanisms of herbs and compounds. Naringin inhibits inflammation and MMPs production and promotes apoptosis in RA-FLS PI3K/Akt and MAPK/ERK signaling pathways.
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http://dx.doi.org/10.3389/fphar.2021.672054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160516PMC
May 2021

Posttranslational Modifications: Emerging Prospects for Cardiac Regeneration Therapy.

J Cardiovasc Transl Res 2021 May 24. Epub 2021 May 24.

Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.

Heart failure (HF) following ischemic heart disease (IHD) remains a hard nut to crack and a leading cause of death worldwide. Cardiac regeneration aims to promote cardiomyocyte (CM) proliferation by transitioning the cell cycle state of CMs from arrest to re-entry. Protein posttranslational modifications (PTMs) have recently attracted extensive attention in the field of cardiac regeneration due to their reversibility and effects on the stability, activity, and subcellular localization of target proteins. The balance of PTMs is disrupted when neonatal CMs withdraw from the cell cycle, resulting in significant dysfunction of downstream substrate protein localization, expression, and activity, ultimately limiting the maintenance of cardiac regeneration ability. In this review, we summarize recent research concerning the role of PTMs in cardiac regeneration, while focusing on phosphorylation, acetylation, ubiquitination, glycosylation, methylation, and neddylation, and the effects of these modifications on CM proliferation, which may provide potential targets for future treatments for IHD.
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http://dx.doi.org/10.1007/s12265-021-10135-7DOI Listing
May 2021

Lead-Free Perovskite Photodetectors: Progress, Challenges, and Opportunities.

Adv Mater 2021 Jul 24;33(26):e2006691. Epub 2021 May 24.

Center for Excellence in Nanoscience (CAS), Key Laboratory of Nanosystem and Hierarchical Fabrication (CAS), National Center for Nanoscience and Technology, Beijing, 100190, China.

State-of-the-art photodetectors which apply hybrid perovskite materials have emerged as powerful candidates for next-generation light sensing. Among them, lead-based ones are the most popular beyond doubt on account of their unique and superior optoelectronic properties. Nevertheless, trade-off toward commercialization exists between nontoxicity and high performance, with the poor stability of lead-based perovskites, indicating that it is indispensable to substitute lead with nontoxic element meanwhile bringing about a comparable figure of merit of photodetectors and relatively long-term stability. Herein, recent advances in lead-free perovskite photodetectors are reviewed, analyzing the principle while designing new materials and highlighting some remarkable progress, which are comparable, even superior, to lead-based photodetectors. Furthermore, their potential strategy in optical communication, image sensing, narrowband photodetection, etc., is examined and a perspective on developing new materials and photodetectors with superior properties for more practical applications is provided.
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http://dx.doi.org/10.1002/adma.202006691DOI Listing
July 2021

Nanobubbles Containing sPD-1 and Ce6 Mediate Combination Immunotherapy and Suppress Hepatocellular Carcinoma in Mice.

Int J Nanomedicine 2021 10;16:3241-3254. Epub 2021 May 10.

Medical College of China Three Gorges University, Yichang, Hubei, People's Republic of China.

Purpose: Immune checkpoint inhibitors (ICIs) and sonodynamic therapy (SDT) are types of immunotherapy. In order to combine soluble programmed cell death protein 1 (sPD-1)-mediated immune checkpoint therapy and chlorin e6 (Ce6)-assisted SDT, nanobubbles (NBs) were generated to simultaneously load sPD-1 and Ce6.

Materials And Methods: The sPD-1/Ce6-NBs, which were prepared by thin-film hydration and mechanical oscillation, had a stable physical condition, and delivered sPD-1 and Ce6 in a targeted manner. NBs could strengthen tumor suppression by increasing tumor-targeting accumulation of Ce6 and sPD-1, and by inducing ultrasound-targeted NB destruction. A mouse H22 cell hepatoma xenograft model was used to evaluate the synergetic immunotherapeutic effect and mechanism of sPD-1/Ce6-NBs.

Results: By observing the tumor inhibition rate, tissue and cell apoptosis, apoptosis-related genes and protein expression, the best immunotherapeutic effect was exhibited by the sPD-1/Ce6-NBs group. The immunotherapeutic mechanism initially demonstrated that when tumor cells were transfected by sPD-1 delivered by NBs, which downregulated the expression of programmed death-ligand 1 (PD-L1) in tumor cells, and blocked the PD-1/PD-L1 signaling pathway, which improved T-cell-mediated tumor inhibition. Furthermore, ICIs combined with SDT induced immunogenic cell death by translocating calreticulin to the cell surface and then synergistically enhancing antitumor immune responses.

Conclusion: In conclusion, sPD-1/Ce6-NBs were successfully designed. Ultrasound-mediated sPD-1/Ce6-NBs are potentially effective delivery systems for combination immunotherapy of hepatocellular carcinoma.
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http://dx.doi.org/10.2147/IJN.S305857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121678PMC
May 2021

Intranasal vaccination with influenza HA/GO-PEI nanoparticles provides immune protection against homo- and heterologous strains.

Proc Natl Acad Sci U S A 2021 May;118(19)

Center for Inflammation, Immunity and Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 30302;

Intranasal (i.n.) immunization is a promising vaccination route for infectious respiratory diseases such as influenza. Recombinant protein vaccines can overcome the safety concerns and long production phase of virus-based influenza vaccines. However, soluble protein vaccines are poorly immunogenic if administered by an i.n. route. Here, we report that polyethyleneimine-functionalized graphene oxide nanoparticles (GP nanoparticles) showed high antigen-loading capacities and superior immunoenhancing properties. Via a facile electrostatic adsorption approach, influenza hemagglutinin (HA) was incorporated into GP nanoparticles and maintained structural integrity and antigenicity. The resulting GP nanoparticles enhanced antigen internalization and promoted inflammatory cytokine production and JAWS II dendritic cell maturation. Compared with soluble HA, GP nanoparticle formulations induced significantly enhanced and cross-reactive immune responses at both systemic sites and mucosal surfaces in mice after i.n. immunization. In the absence of any additional adjuvant, the GP nanoparticle significantly boosted antigen-specific humoral and cellular immune responses, comparable to the acknowledged potent mucosal immunomodulator CpG. The robust immune responses conferred immune protection against challenges by homologous and heterologous viruses. Additionally, the solid self-adjuvant effect of GP nanoparticles may mask the role of CpG when coincorporated. In the absence of currently approved mucosal adjuvants, GP nanoparticles can be developed into potent i.n. influenza vaccines, providing broad protection. With versatility and flexibility, the GP nanoplatform can be easily adapted for constructing mucosal vaccines for different respiratory pathogens.
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http://dx.doi.org/10.1073/pnas.2024998118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126854PMC
May 2021

Alkylamide Profiling of Pericarps Coupled with Chemometric Analysis to Distinguish Prickly Ash Pericarps.

Foods 2021 Apr 15;10(4). Epub 2021 Apr 15.

College of Forestry, Northwest A&F University, Yangling 712100, China.

Because of extensive cultivation areas, various cultivars, nonstandard naming notations, and morphology similarity among relative cultivars, adulteration and associated business fraud may happen in the marketplaces of prickly ash pericarps due to higher financial gain and high-frequency trading. This study presents variations in the chemical components and contents of different prickly ash species from different plantations. Alkylamide profiling of pericarps derived from , and some relative species from 72 plantations across China were tested using ultra-performance liquid chromatography. Then, several chemometrics were applied to classify the prickly ash pericarps to reveal potential indicators that distinguish prickly ash pericarps and to identify the key factors that affect pericarp alkylamide profiling. The dominating alkylamides in the prickly ash pericarps were (ZP)-amide C (0-20.64 mg/g) and ZP-amide D (0-30.43 mg/g). Alkylamide profiling of prickly ash pericarps varied significantly across species and geographical variations. ZP-amide D in prickly ash pericarps was identified as a potential indicator to distinguish prickly ash species. Longitude and aluminum content in soils were identified as key factors that affected alkylamide profiling of prickly ash pericarps. This study provides a useful tool to classify prickly ash species based on pericarp alkylamide profiling and to determine the key influence factors on pericarp alkylamide variations.
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http://dx.doi.org/10.3390/foods10040866DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071439PMC
April 2021

Can YKL-40 be used as a biomarker for interstitial lung disease?: A systematic review and meta-analysis.

Medicine (Baltimore) 2021 Apr;100(17):e25631

Department of Respiratory Medicine and Critical Care Medicine, West China Hospital/West China School of Medicine, Sichuan University.

Background: Interstitial lung disease (ILD) has a poor prognosis and lacks specific biomarkers for early diagnosis, assessment of disease severity, and prognosis. YKL-40 levels were found to be elevated in patients with ILD, but these results are inconsistent. Therefore, we conducted a systematic review and meta-analysis to accurately study the relation between YKL-40 and ILD.

Methods: We performed a systematic literature search in many databases (PubMed, Embase, the China National Knowledge Infrastructure, and Wanfang databases) and commercial Internet search engines to identify studies involving the role of YKL-40 in patients with ILD. The weighted mean difference with its 95% confidence interval were used to investigate the effect sizes. If obvious heterogeneity was found in the meta-analysis, the level of YKL-40 was directly compared by the Mann-Whitney test.

Results: Sixteen eligible articles were finally identified. The results showed that the serum YKL-40 levels of patients with idiopathic pulmonary fibrosis, connective tissue-related ILD, sarcoidosis, cryptogenic tissue pneumonia, asbestosis-ILD, and idiopathic nonspecific interstitial pneumonia were higher than those in controls, but there was no increase in patients with pulmonary alveolar proteinosis. We also found that there are certain differences in the serum YKL-40 levels in patients with different types of ILD. The results showed that the bronchoalveolar lavage fluid YKL-40 levels of patients with idiopathic pulmonary fibrosis were significantly higher than that in controls. A systematic review indicated that there were correlations between the serum YKL-40 levels and lung function in patients with different ILD. In addition, YKL-40 may be used as a valuable biomarker for survival, with risk ratios ranging from 1.006 to 10.9.

Conclusions: This study suggests that YKL-40 may be a useful biomarker for the diagnosis and prognosis of ILD.
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http://dx.doi.org/10.1097/MD.0000000000025631DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083999PMC
April 2021

High-Temperature Stable FAPbBr Single Crystals for Sensitive X-ray and Visible Light Detection toward Space.

Nano Lett 2021 May 21;21(9):3947-3955. Epub 2021 Apr 21.

State Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, P. R. China.

Organolead trihalide perovskite single crystals (SCs) offer unprecedented opportunity for X-ray and visible light detection. Nevertheless, it remains a challenge to keep simultaneous high-performance and stability at a high-temperature working mode. Herein, formamidinium lead bromide (FAPbBr) SCs are developed to successfully address these issues. Low-temperature crystallized induced FAPbBr SCs possess an excellent mobility-lifetime product and an ultralow surface charge recombination velocity, thus exhibiting an X-ray dose rate as low as 0.3 μGy s as a sensitive radiation detector. Furthermore, it also contributes a specific detectivity as high as 3.5 × 10 cm Hz W, keeping stable at high-temperature of 460 K as a photodetector. A prototype of an imaging system with diffuse reflection mode is constructed using detectors as receivers, enabling defined scanning images in a high temperature environment. The bifunctional FAPbBr SC detectors will motivate new strategies for stable detection in an extreme space environment.
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http://dx.doi.org/10.1021/acs.nanolett.1c00700DOI Listing
May 2021

Synergistic anti-tumor effect of anti-PD-L1 antibody cationic microbubbles for delivery of the miR-34a gene combined with ultrasound on cervical carcinoma.

Am J Transl Res 2021 15;13(3):988-1005. Epub 2021 Mar 15.

Medical College of China Three Gorges University Yichang, China.

This study explored the synergistic effect of anti-PD-L1 antibody cationic microbubbles (MBs) for delivery of the miR-34a gene combined with ultrasound in inhibiting the cervical cancer. H&E stain, TUNEL, immunohistochemistry and RT-PCR were used to detect the change of apoptosis regulatory factors, and immunofluorescence, Flow cytometry and LDH assays were applied to evaluate the changing of immunomodulatory. In this experiment the PD-L1 Ab/miR-34a-MBs were prepared successfully. The cell targeting assay showed that U14 cells were surrounded by the PD-L1 Ab/miR-34a-MBs and microbubbles had well contrast imaging capability in vivo. With the irradiation power was 1 W/cm and the irradiation time was 25 s, the gene transfection efficiency was the highest using EGFP plasmid lorded microbubbles. In vivo anti-tumor assays, the PD-L1 Ab/miR-34a-MBs showed a great potential in inhibiting tumor growth with a TGI of >50%. PD-L1 Ab/miR-34a-MBs treatment enhanced the anti-tumor effect compared with that induced by PD-L1 Ab or miR-34a alone. Firstly, PD-L1 Ab/miR-34a-MBs could gather miR-34a with high-concentration aggregation and releasing around the cervical cancer, which takes a significant role in promoting apoptosis by downregulated Bcl-2 and upregulated Bax. Furthermore, combination therapy was found to augment the activation of T lymphocytes proliferation and increase CD8+ T cells infiltration, to enhance antitumor immune killing effect. The anti-PD-L1 antibody microbubbles for delivery miR-34a gene with ultrasound were considered to be a promising combination therapy regimen via initiating apoptotic mechanism of the tumor and anti-tumor immune regulation.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8014418PMC
March 2021

Factors Controlling the Lower Radioactivity and Its Relation with Higher Organic Matter Content for Middle Jurassic Oil Shale in Yuqia Depression, Northern Qaidam Basin, China: Evidence from Organic and Inorganic Geochemistry.

ACS Omega 2021 Mar 8;6(11):7360-7373. Epub 2021 Mar 8.

Qinghai Geological Survey, Xining 810012, China.

Organic-rich oil shale with unusual lower radioactivity (expressed by GR) was found in Member 7 of Dameigou Formation, middle Jurassic (Jd) in Yuqia depression of northern Qaidam Basin, China. In order to systematically and contrastively investigate the factors controlling the lower GR and its relation with higher organic matter (OM) content (expressed by total organic carbon, abbr. TOC), organic and inorganic geochemical analyses were performed on samples consisting of oil shale and the underlying conformable contact dark shale from Well YQ-1Y. Our study shows that GR of Jd oil shale is mainly derived from uranium and thorium. Compared with dark shale, oil shale is characterized by higher OY and TOC, lower GR, and clay mineral content. During oil shale deposition, the paleoclimate was relatively arid, indicated by a decreased C value and siderite content as well as an increased carbonate content and . Under such paleoclimate conditions, sedimentary water became more anoxic, suggested by higher V/(V + Ni), pyrite content and lower pristane/phytane (Pr/Ph). From oil shale to dark shale deposition, according to analyses of AlO/TiO, TiO versus Zr, La/Sc versus Th/Co, La/Th versus Hf, and La-Th-Sc, the felsic igneous rock could always be deduced as the parent rock of provenance; however, the increasing arid paleoclimate resulted in weakened chemical weathering of provenance, inferred by relatively low chemical index of alteration, chemical index of weathering, and plagioclase index of alteration corresponding to the input degree of radioactive materials and other terrigenous detrital materials (TDMs), evidenced by Ti and Al contents and terrigenous (%). Meanwhile, the relatively high P/Ti and Ba/Al both indicated increased primary paleoproductivity. Together with the maximum flooding stage of oil shale deposition, the relatively low radioactivity tends to be associated with the inhibited input of clay minerals and radioactive materials, largely caused by increasing arid paleoclimate. The accompanying decreased TDM benefited primary paleoproductivity and anoxic conditions; their combined influence could induce sapropelic OM accumulation.
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http://dx.doi.org/10.1021/acsomega.0c05618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992071PMC
March 2021

Ultra-Narrow-Band NIR Photomultiplication Organic Photodetectors Based on Charge Injection Narrowing.

J Phys Chem Lett 2021 Mar 16;12(11):2937-2943. Epub 2021 Mar 16.

Key Laboratory of Luminescence and Optical Information, Ministry of Education, Beijing Jiaotong University, Beijing 100044, P. R. China.

Ultra-narrow-band NIR photomultiplication organic photodetectors (PM-OPDs) were realized in ITO/PEDOT:PSS/active layers/Al based on an interfacial-trap-induced charge injection narrowing (CIN) concept. The rather less Bod Ethex-Hex (BEH) is imbedded in a polymer donor matrix to form large amounts of isolated electron traps. Trapped electrons in BEH close to an Al electrode will enforce hole-tunneling injection induced by interfacial band bending, resulting in a photomultiplication phenomenon. PM-OPDs with P3HT:BEH as the active layer exhibit a narrow response peak at 850 nm with a full-width at half-maximum (fwhm) of 27 nm as well as a rather weak response from 650 to 800 nm. The EQE of 29 700% at 850 nm was achieved in PM-OPDs by incorporating 0.02 wt % of FTCNNQ under -13 V of applied voltage. The rejection ratio (RR) of the optimized PM-OPDs with FTCNNQ is 11 for EQE/EQE and 10 for EQE/EQE, respectively. An EQE of 15 300% at 850 nm was achieved in the ternary PM-OPDs under -13 V of applied voltage, with markedly enhanced RRs of 44 for EQE/EQE and 30 for EQE/EQE.
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http://dx.doi.org/10.1021/acs.jpclett.1c00330DOI Listing
March 2021

Transmission electron microscopy sample preparation method for micrometer-sized powder particles using focused ion beam.

Micron 2021 Apr 5;143:103030. Epub 2021 Feb 5.

Vacuum Interconnected Nanotech Workstation (Nano-X), Suzhou Institute of Nano-Tech and Nano-Bionics (SINANO), Chinese Academy of Sciences (CAS), Suzhou, 215123, China. Electronic address:

A TEM sample preparation technique for micrometer-sized powder particles in the 1-10 μm size range is proposed, using a focused ion beam (FIB) system. It is useful for characterizing elemental distributions across an entire cross-section of a particle. It is a simple and universal method without using any embedding agent, enabling the powder particles with different size, shape or orientation to be easily selected based on the SEM observations. The suitable particle is covered with Pt coating layers through an ion-beam-assisted deposition. The Pt coating layers provide sufficient support for the TEM lamella. A small piece of tungsten needle is used as a support under the particle by taking a series of operations using a micromanipulator. The particle can be precisely thinned by the ion beam to be suitable for both TEM observation and EDX elemental mapping. This novel technique reduces the TEM sample preparation time to a few hours, allowing much higher efficiency compared to complicated and time-consuming embedding methods.
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http://dx.doi.org/10.1016/j.micron.2021.103030DOI Listing
April 2021

The Role of TSC1 in the Macrophages Against Infection.

Front Cell Infect Microbiol 2020 27;10:596609. Epub 2021 Jan 27.

Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.

() is an estuarine bacterium that is capable of causing rapidly fatal infection in humans. Proper polarization and bactericidal activity of macrophages play essential roles in defending against invading pathogens. How macrophages limit infection remains not well understood. Here we report that tuberous sclerosis complex 1 (TSC1) is crucial for the regulation of -induced macrophage polarization, bacterial clearance, and cell death. Mice with myeloid-specific deletion of TSC1 exhibit a significant reduction of survival time after infection. infection induces both M1 and M2 polarization. However, TSC1 deficient macrophages show enhanced M1 response to infection. Interestedly, the absence of TSC1 in myeloid cells results in impaired bacterial clearance both and after infection. Inhibition of the mammalian target of rapamycin (mTOR) activity significantly reverses -induced hypersensitive M1 response and resistant bactericidal activity both in wild-type and TSC1-deficient macrophages. Moreover, infection causes cell death of macrophages, possibly contributes to defective of bacterial clearance, which also exhibits in a mTORC1-dependent manner. These findings highlight an essential role for the TSC1-mTOR signaling in the regulation of innate immunity against infection.
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http://dx.doi.org/10.3389/fcimb.2020.596609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873526PMC
June 2021

Membrane Sterol Composition in Affects Root Elongation via Auxin Biosynthesis.

Int J Mol Sci 2021 Jan 4;22(1). Epub 2021 Jan 4.

Department of Plant Biology and Ecology, College of Life Sciences, Nankai University and Tianjin Key Laboratory of Protein Sciences, Tianjin 300071, China.

Plant membrane sterol composition has been reported to affect growth and gravitropism via polar auxin transport and auxin signaling. However, as to whether sterols influence auxin biosynthesis has received little attention. Here, by using the sterol biosynthesis mutant () and sterol application, we reveal that cycloeucalenol, a CPI1 substrate, and sitosterol, an end-product of sterol biosynthesis, antagonistically affect auxin biosynthesis. The short root phenotype of was associated with a markedly enhanced auxin response in the root tip. Both were neither suppressed by mutations in polar auxin transport (PAT) proteins nor by treatment with a PAT inhibitor and responded to an auxin signaling inhibitor. However, expression of several auxin biosynthesis genes () was upregulated in Functionally, mutation reduced the auxin response in and partially rescued its short root phenotype. In support of this genetic evidence, application of cycloeucalenol upregulated expression of the auxin responsive reporter (β-glucuronidase) and of several auxin biosynthesis genes, while sitosterol repressed their expression. Hence, our combined genetic, pharmacological, and sterol application studies reveal a hitherto unexplored sterol-dependent modulation of auxin biosynthesis during Arabidopsis root elongation.
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http://dx.doi.org/10.3390/ijms22010437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794993PMC
January 2021

Solvents-dependent selective fabrication of face-centered cubic and hexagonal close-packed structured ruthenium nanoparticles during liquid-phase laser ablation.

J Colloid Interface Sci 2021 Mar 12;585:452-458. Epub 2020 Oct 12.

Key Laboratory of Materials Physics and Anhui Key Laboratory of Nanomaterials and Nanotechnology, Institute of Solid State Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China; Department of Materials Science and Engineering, University of Science and Technology of China, Hefei 230026, China.

Ruthenium nanoparticles (Ru NPs) with face-centered cubic (fcc) structure possess higher catalytic activity than that with hexagonal close-packed (hcp) structure. However, a high temperature above 1800 K is needed for the formation of the metastable fcc Ru phase. In this study, we present a tunable fabrication strategy of fcc and hcp Ru NPs by laser ablation of Ru target in solvents. In methanol, ethanol or acetone organic solvent, both fcc and hcp Ru NPs encapsulated in carbon layer could be obtained, while in deionized water only pure hcp Ru NPs formed. The extreme conditions, that is, the laser-target interaction induced high temperature and high-pressure plasma plume (4000-5000 K, 10-15 GPa) together with its subsequent quenching process, favored the formation of metastable fcc phase. Significantly, the graphite carbon layers sourced from the thermal decomposition of solvent molecules prevent the further evolution of metastable fcc phase into stable hcp phase. Clarification of the solvents and pulse energy effects promise the tunable fabrication of Ru NPs with desired crystallographic structure during laser ablation in liquids (LAL).
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http://dx.doi.org/10.1016/j.jcis.2020.10.026DOI Listing
March 2021

Planar Heterojunction Organic Photodetectors Based on Fullerene and Non-fullerene Acceptor Bilayers for a Tunable Spectral Response.

ACS Appl Mater Interfaces 2020 Dec 24;12(49):55064-55071. Epub 2020 Nov 24.

College of Materials Science and Engineering, Key Laboratory for Micro-Nano Physics and Technology of Hunan Province, Hunan University, Changsha, Hunan 410082, China.

Planar heterojunction (PHJ) organic photodetectors are potentially more stable than traditional bulk heterojunction counterparts because of the absence of uncontrolled phase separation in the donor and acceptor binary blend system. This work reports a new class of PHJ organic photodetectors based on the medium-band gap fullerene C and low-band gap fused-ring non-fullerene acceptor ID-MeIC bilayer structure, which allows a wide range of spectral response tuning across the UV-visible-near-infrared (UV-vis-NIR) region by tailoring individual layer thickness. The C layer not only increases the external quantum efficiency at 745 nm by 57% but also reduces dark currents by two orders of magnitude. More importantly, the p-type poly[,'-bis(4-butylphenyl)-,'-bis(phenyl)-benzi] is found to be the key compound to conduct the layer-by-layer fabrication as combined with n-type ID-MeIC for higher charge extraction efficiency. In light of the above information, PHJ organic photodetectors exhibited a specific detectivity of 6.5 × 10 Jones to detect NIR light at 745 nm under -0.1 V. The linear dynamic range was estimated to be 80 dB. This work has demonstrated a feasible approach to develop a PHJ architecture with tunable spectral response in the UV-vis-NIR range toward long-term stable organic photodetectors for potential applications in flexible and wearable biomedical sensors.
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http://dx.doi.org/10.1021/acsami.0c16192DOI Listing
December 2020

Aptamer-functionalized magnetic nanoparticles conjugated organic framework for immobilization of acetylcholinesterase and its application in inhibitors screening.

Anal Chim Acta 2020 Dec 16;1140:228-235. Epub 2020 Oct 16.

Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing, 100081, China. Electronic address:

Construction of new enzyme reactor based on aptamer functionalized magnetic nanoparticles conjugated organic framework (COF) for acetylcholinesterase immobilization has been an enabling endeavor in this work. The aptamer against acetylcholinesterase was selected through a method based on capillary electrophoresis in one round. A new magnetic COF material rich of carboxyl groups was firstly synthesized, and its surface was then modified with the selected aptamer through covalently linking. Acetylcholinesterase was immobilized to fabricate the enzyme reactor [email protected] through the high affinity and specificity with its binding aptamer. The as constructed enzyme reactor was comprehensively characterized and the key factors that affected its catalysis efficiency were investigated in detail. Owing to the surface modification of the magnetic COF materials by aptamer for acetylcholinesterase immobilization, the immobilized enzyme exhibited improved substrates affinity. What's more, good reusability (more than 8 times) and prolonged stability (enzyme activity still kept at 90% after 42 days) were also achieved. Finally, the enzyme reactor could be applied in AChE inhibitors screening, which expanded its application capability. The proposed protocol not only paves a new way for fabrication of novel aptamer functionalized magnetic COF materials as enzyme reactors, but also indicates a broadened application of the integration of aptamer and its enzyme.
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http://dx.doi.org/10.1016/j.aca.2020.10.024DOI Listing
December 2020

Fatty Acid Profiling and Chemometric Analyses for Pericarps from Different Geographic Origin and Genotype.

Foods 2020 Nov 16;9(11). Epub 2020 Nov 16.

College of Forestry, Northwest A&F University, Yangling 712100, China.

plants, important aromatic plants, have attracted considerable attention in the food, pharmacological, and industrial fields because of their potential health benefits, and they are easily accessible because of the wild distribution in most parts of China. The chemical components vary with inter and intraspecific variations, ontogenic variations, and climate and soil conditions in compositions and contents. To classify the relationships between different species and to determine the key factors that influence geographical variations in the main components of the plant, the fatty acid composition and content of 72 pericarp samples from 12 cultivation regions were measured and evaluated. Four fatty acids, palmitic acid (21.33-125.03 mg/g), oleic acid (10.66-181.37 mg/g), linoleic acid (21.98-305.32 mg/g), and linolenic acid (0.06-218.84 mg/g), were the most common fatty acid components in the pericarps. Fatty acid profiling of pericarps was significantly affected by species and geographical variations. Stearic acid and oleic acid in pericarps were typical fatty acids that distinguished species based on the result of DA. Palmitic acid, palmitoleic acid, trans-13-oleic acid, and linoleic acid were important differential indicators in distinguishing given pericarps based on the result of OPLS-DA. In different species, the geographical influence on fatty acid variations was diverse. This study provides information on how to classify the species based on pericarp fatty acid compositions and determines the key fatty acids used to classify the species.
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http://dx.doi.org/10.3390/foods9111676DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698129PMC
November 2020

MloDisDB: a manually curated database of the relations between membraneless organelles and diseases.

Brief Bioinform 2021 Jul;22(4)

Department of Biomedical Informatics, Peking University Health Science Center, Beijing, China.

Cells are compartmentalized by numerous membrane-bounded organelles and membraneless organelles (MLOs) to ensure temporal and spatial regulation of various biological processes. A number of MLOs, such as nucleoli, nuclear speckles and stress granules, exist as liquid droplets within the cells and arise from the condensation of proteins and RNAs via liquid-liquid phase separation (LLPS). By concentrating certain proteins and RNAs, MLOs accelerate biochemical reactions and protect cells during stress, and dysfunction of MLOs is associated with various pathological processes. With the development in this field, more and more relations between the MLOs and diseases have been described; however, these results have not been made available in a centralized resource. Herein, we build MloDisDB, a database which aims to gather the relations between MLOs and diseases from dispersed literature. In addition, the relations between LLPS and diseases were included as well. Currently, MloDisDB contains 771 curated entries from 607 publications; each entry in MloDisDB contains detailed information about the MLO, the disease and the functional factor in the relation. Furthermore, an efficient and user-friendly interface for users to search, browse and download all entries was provided. MloDisDB is the first comprehensive database of the relations between MLOs and diseases so far, and the database is freely accessible at http://mlodis.phasep.pro/.
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http://dx.doi.org/10.1093/bib/bbaa271DOI Listing
July 2021

NLRP3 and mTOR Reciprocally Regulate Macrophage Phagolysosome Formation and Acidification Against Infection.

Front Cell Dev Biol 2020 8;8:587961. Epub 2020 Oct 8.

Department of Microbiology and Immunology, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.

The marine bacterium causes potentially fatal bloodstream infections, typically in patients with chronic liver diseases. The inflammatory response and anti-bacterial function of phagocytes are crucial for limiting bacterial infection in the human hosts. How affects macrophages after phagocytosis is unclear. In this report, we found that the bactericidal activity of macrophages to internalize was dependent on mammalian target of rapamycin (mTOR) and NOD-like receptor (NLR) family pyrin domain containing 3 (NLRP3) interaction. Additionally, the NLRP3 expression was dependent on mTORC1 activation. Inhibited mTORC1 or absence of NLRP3 in macrophages impaired -induced phagosome acidification and phagolysosome formation, leading to a reduction of intracellular bacterial clearance. mTORC1 signaling overactivation could increase NLRP3 expression and restore insufficient phagosome acidification. Together, these findings indicate that the intracellular bactericidal activity of macrophages responding to infection is tightly controlled by the crosstalk of NLRP3 and mTOR and provide critical insight into the host bactericidal activity basis of clearance of through lyso/phagosome.
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http://dx.doi.org/10.3389/fcell.2020.587961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578225PMC
October 2020

Experimental Studies on the Dynamic Memcapacitance Modulation of the [email protected] Composite Material-Based Diode.

Nanomaterials (Basel) 2020 Oct 23;10(11). Epub 2020 Oct 23.

College of Physics, Sichuan University, Chengdu 610064, China.

In this study, both memcapacitive and memristive characteristics in the composite material based on the rhenium disulfide (ReS) rich in rhenium (VI) oxide (ReO) surface overlayer ([email protected]) and in the indium tin oxide (ITO)/[email protected]/aluminum (Al) device configuration is presented. Comprehensive experimental analysis of the [email protected] material properties' dependence on the memcapacitor electrical characteristics was carried out by standard as well as frequency-dependent current-voltage, capacitance-voltage, and conductance-voltage studies. Furthermore, determination of the charge carrier conduction model, charge carrier mobility, density of the trap states, density of the available charge carrier, free-carrier concentration, effective density of states in the conduction band, activation energy of the carrier transport, as well as ion hopping was successfully conducted for the [email protected] based on the experimental data. The ITO/[email protected]/Al charge carrier conduction was found to rely on the mixed electronic-ionic processes, involving electrochemical metallization and lattice oxygen atoms migration in response to the externally modulated electric field strength. The chemical potential generated by the electronic-ionic ITO/[email protected]/Al resistive memory cell non-equlibrium processes leads to the occurrence of the nanobattery effect. This finding supports the possibility of a nonvolatile memory cell with a new operation principle based on the potential read function.
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http://dx.doi.org/10.3390/nano10112103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690752PMC
October 2020
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