Publications by authors named "Yao Lu"

1,111 Publications

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Fast liquid chromatography method for separation of peptides using a sub-2 μm ground silica monolith packed column.

J Sep Sci 2021 Sep 17. Epub 2021 Sep 17.

Department of pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, China.

A stationary phase based on sub-2 μm ground silica monolith particles was fabricated by in situ polymerization and applied in micro-column for separation of peptides. The sub-2 μm silica particles were obtained from monolith using sol-gel process followed by grinding and sedimentation to remove the fines. Initially, the silica monolith particles were pretreated with 3-trimethoxysilyl propyl methacrylate to attach double-bonded ligands onto the surface, then a network structure was formed onto the surface of the particle using styrene, N-isopropylacrylamide and ethylene glycoldimethacrylate. The effect of the flow rate of mobile phase on the separation performance was investigated. The stationary phase was characterized by field emission scanning electron microscopy, thermogravimetry, particle size distribution and element analysis. The resultant phase was packed in glass lined stainless steel micro-columns (2.1 mm x 50 mm) and evaluated for fast separation. An average number of theoretical plates as high as 9800 plates/column (5.10 μm plate height) was achieved for five synthetic peptides under the optimized flow rate of 0.15 mL/min. The repeatabilities of column-to-column, intra-day and inter-day through relative standard deviation were found better than 4%, exhibiting satisfactory repeatability of the developed micro-column for fast separation of peptides. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jssc.202100504DOI Listing
September 2021

Higher dietary insulinaemic potential is associated with increased risk of liver steatosis and fibrosis.

Liver Int 2021 Sep 14. Epub 2021 Sep 14.

Department of Nutrition, School of Public Health, Anhui Medical University, Hefei, China.

Background And Aims: Hyperinsulinaemia and insulin resistance play a central role in the progression of hepatic steatosis and fibrosis, and diet can modulate insulin response. We thus hypothesised that diet with higher insulinaemic potential is associated with an increased risk of these conditions.

Methods: Two empirically dietary indices for hyperinsulinaemia (EDIH) and insulin resistance (EDIR) were derived to identify food groups most predictive of fasting concentrations of C-peptide and insulin and homeostatic model assessment for insulin resistance respectively. Hepatic steatosis and fibrosis were defined by controlled attenuation parameter and liver stiffness measurement using transient elastography (TE). Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated by logistic regression.

Results: Of the 4171 participants with TE examination, 1436 (age-standardised prevalence, 33.8%) were diagnosed with steatosis, 255 (5.6%) with advanced fibrosis and 101 (2.2%) with cirrhosis. The multivariable-adjusted ORs for participants comparing the highest to the lowest EDIH tertile were 1.17 (95% CI: 0.99-1.39, P  = .005) for steatosis, 1.74 (95% CI: 1.24-2.44, P  = .001) for advanced fibrosis and 2.05 (95% CI: 1.21-3.46, P  = .004) for cirrhosis. Similar associations were observed for EDIR with ORs of 1.32 (95% CI: 1.11-1.55, P  < .001) for steatosis and 1.43 (95% CI: 1.03-1.99, P  = .006) for advance fibrosis. These positive associations remained among never drinkers and individuals who were free of hepatitis B and/or C.

Conclusions: Our findings suggest that hyperinsulinaemia and insulin resistance may partially underlie the influence of diet on hepatic steatosis and fibrosis, and highlight the importance of reducing or avoiding insulinaemic dietary pattern.
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http://dx.doi.org/10.1111/liv.15057DOI Listing
September 2021

Cardio- and Cerebrovascular Outcomes of Postoperative Acute Kidney Injury in Noncardiac Surgical Patients With Hypertension.

Front Pharmacol 2021 27;12:696456. Epub 2021 Aug 27.

Center of Clinical Research, The Third Xiangya Hospital, Central South University, Changsha, China.

The cardiovascular and cerebrovascular risk of postoperative acute kidney injury (AKI) in surgical patients is poorly described, especially in the hypertensive population. : We conducted a retrospective cohort study among all hypertensive patients who underwent elective noncardiac surgery from January 1st, 2012 to August 1st, 2017 at the Third Xiangya Hospital. The primary outcomes were fatal stroke and fatal myocardial infarction (MI). The secondary outcomes were all-cause mortality. The postoperative cumulative mortality within 3 months, 6 months, 1 year, 2 years, and 5 years were 1.27, 1.48, 2.15, 2.15, and 5.36%, for fatal stroke, and 2.05, 2.27, 2.70, 3.37, and 5.61% for fatal MI, respectively, in patients with postoperative AKI. Compared with non-AKI patients, those with postoperative AKI had a significantly higher risk of fatal stroke and fatal MI within 3 months [hazard ratio (HR): 5.49 (95% CI: 1.88-16.00) and 11.82 (95% CI: 4.56-30.62), respectively], 6 months [HR: 3.58 (95% CI: 1.43-8.97) and 9.23 (95% CI: 3.89-21.90), respectively], 1 year [HR: 3.64 (95% CI: 1.63-8.10) and 5.14 (95% CI: 2.50-10.57), respectively], 2 years [HR: 2.21 (95% CI: 1.03-4.72) and 3.06 (95% CI: 1.66-5.64), respectively], and 5 years [HR: 2.27 (95% CI: 1.30-3.98) and 1.98 (95% CI: 1.16-3.20), respectively]. In subgroup analysis of perioperative blood pressure (BP) lowering administration, postoperative AKI was significantly associated with 1-year and 5-year risk of fatal stroke [HR: 9.46 (95% CI: 2.85-31.40) and 3.88 (95% CI: 1.67-9.01), respectively] in patients with ACEI/ARB, and MI [HR: 6.62 (95% CI: 2.23-19.62) and 2.44 (95% CI: 1.22-4.90), respectively] in patients with CCB. Hypertensive patients with postoperative AKI have a significantly higher risk of fatal stroke and fatal MI, as well as all-cause mortality, within 5 years after elective noncardiac surgery. In patients with perioperative administration of ACEI/ARB and CCB, postoperative AKI was significantly associated with higher risk of fatal stroke and MI, respectively.
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http://dx.doi.org/10.3389/fphar.2021.696456DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430207PMC
August 2021

Nontargeted identification of chlorinated disinfection byproducts formed from natural organic matter using Orbitrap mass spectrometry and a halogen extraction code.

J Hazard Mater 2021 08 26;416:126198. Epub 2021 May 26.

Key Laboratory of Microorganism Application and Risk Control of Shenzhen, Guangdong Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Institute of Environment and Ecology, Tsinghua-Berkeley Shenzhen Institute, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, PR China; Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, PR China. Electronic address:

Natural organic matter is a major source of precursors of hazardous chlorinated disinfection byproducts (Cl-DBPs) formed during water treatment, but the majority of Cl-DBPs are still unidentified. In this study, we used a self-written halogen extraction code to identify halogen isotopic patterns in combination with the R package MFAssignR, to identify Cl-DBPs from Orbitrap mass spectra. One hundred and eighty-nine Cl-DBPs were detected during chlorination of a Suwannee River natural organic matter solution, and the structures of 20 of these compounds are reported for the first time. Kendrick mass defect analysis and structural identification confirmed that chlorinated carboxylic acids are common and likely to form during chlorination. A toxicity prediction using quantitative structure-activity relationship models indicated that most of the chlorinated carboxylic acids may be highly toxic. Our analytical strategy can identify Cl-DBPs accurately from complex mixtures and may also be applicable to the identification of other halogenated disinfection byproducts formed during water treatment.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126198DOI Listing
August 2021

Comparison Study of Self-Cleaning, Anti-Icing, and Durable Corrosion Resistance of Superhydrophobic and Lubricant-Infused Ultraslippery Surfaces.

Langmuir 2021 Sep 7. Epub 2021 Sep 7.

Department of Chemistry, School of Biological and Chemical Sciences, Queen Mary University of London, London E1 4NS, U.K.

Endowing metallic surfaces with special wettability and unique interfacial contacts broadens their wide application fields. Herein, superhydrophobic and lubricant-infused ultraslippery surfaces were achieved through chemical etching, low surface energy molecule grafting, and lubricant infusion. Systematic comparison studies of the surface wettability, self-cleaning, anti-icing, anticorrosion behaviors, and mechanical durability were carried out to reveal the functional differences and mechanisms. Both superhydrophobic and ultraslippery surfaces exhibit a distinct decrease in ice adhesion strength and a remarkable increase in charge-transfer resistance, demonstrating significantly improved ice overdelay and corrosion-resisting performance. Most notably, given the existence of a stable, defect-free, and inert lubricant-infused layer, the lubricant-infused ultraslippery surfaces possess superior mechanical robustness and long-term corrosion resistance, which provides better application potential under challenging service environments.
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http://dx.doi.org/10.1021/acs.langmuir.1c01684DOI Listing
September 2021

Structure evolution and energy storage mechanism of ZnVO spinel in aqueous zinc batteries.

Nanoscale 2021 Sep 2;13(34):14408-14416. Epub 2021 Sep 2.

School of Advanced Materials, Peking University Shenzhen Graduate School, Shenzhen 518055, China.

Spinel-type materials are promising for the cathodes in rechargeable aqueous zinc batteries. Herein, ZnVO is synthesized a simple solid-state reaction method. By tuning the Zn(CFSO) concentration in electrolytes and the cell voltage ranges, improved electrochemical performance of ZnVO can be achieved. The optimized test conditions give rise to progressive structure evolution from bulk to nano-crystalline spinel, which leads to capacity activation in the first few cycles and stable cycling performance afterward. Furthermore, the energy storage mechanism in this nano-crystalline spinel is interpreted as the co-intercalation of zinc ions and protons with some water. This work provides a new viewpoint of the structure evolution and correlated energy storage mechanism in spinel-type host materials, which would benefit the design and development of next-generation batteries.
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http://dx.doi.org/10.1039/d1nr02347kDOI Listing
September 2021

Robust Micro-Nanostructured Superhydrophobic Surfaces for Long-Term Dropwise Condensation.

Nano Lett 2021 Sep 2. Epub 2021 Sep 2.

College of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China.

Design of hierarchical micromorphology represents an important strategy for developing functional surfaces but has yet to be achieved for promising long-term dropwise condensation. Herein, micropapillaes overlaid with nanograss were created to enhance dropwise condensation. By analyzing the nucleation and evolution of the condensate droplets, we elucidated that these hierarchical micro-nanostructures topologized tapered gaps, which produced upward pressure, to achieve spontaneous dislodging of condensate microdroplet out of gaps, and then to trigger microdroplet navigation before finally departing from the surface by coalescence-induced jumping. The high mobility of condensate delayed flooding and contributed to a very high heat transfer coefficient of 218 kW·m·K. Moreover, these micropapillaes served as forts that protected the nanograss from being destroyed, resulting in improved mechanical and chemical robustness. Our work proposed new examples of topology creation for long-term dropwise condensation heat transfer and shed light on application integration of such promising functional surfaces.
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http://dx.doi.org/10.1021/acs.nanolett.1c01584DOI Listing
September 2021

Plaque Character and Progression According to the Location of Coronary Atherosclerotic Plaque.

Am J Cardiol 2021 Aug 28. Epub 2021 Aug 28.

Cardiovascular Imaging Unit, SDN IRCCS, Naples, Italy.

Although acute coronary syndrome culprit lesions occur more frequently in the proximal coronary artery, whether the proximal clustering of high-risk plaque is reflected in earlier-stage atherosclerosis remains unclarified. We evaluated the longitudinal distribution of stable atherosclerotic lesions on coronary computed tomography angiography (CCTA) in 1,478 patients (mean age, 61 years; men, 58%) enrolled from a prospective multinational registry of consecutive patients undergoing serial CCTA. Of 3,202 coronary artery lesions identified, 2,140 left lesions were classified (based on the minimal lumen diameter location) into left main (LM, n = 128), proximal (n = 739), and other (n = 1,273), and 1,062 right lesions were classified into proximal (n = 355) and other (n = 707). Plaque volume (PV) was the highest in proximal lesions (median, 26.1 mm), followed by LM (20.6 mm) and other lesions (15.0 mm, p <0.001), for left lesions, and was lager in proximal (25.8 mm) than in other lesions (15.2 mm, p <0.001) for right lesions. On both sides, proximally located lesions tended to have greater necrotic core and fibrofatty components than other lesions (left: LM, 10.6%; proximal, 5.8%; other, 3.4% of the total PV, p <0.001; right: proximal, 8.4%; other 3.1%, p <0.001), with less calcified plaque component (left: LM, 18.3%; proximal, 30.3%; other, 37.7%, p <0.001; right: proximal, 23.3%, other, 36.6%, p <0.001), and tended to progress rapidly (adjusted odds ratios: left: LM, reference; proximal, 0.95, p = 0.803; other, 0.64, p = 0.017; right: proximal, reference; other, 0.52, p <0.001). Proximally located plaques were larger, with more risky composition, and progressed more rapidly.
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http://dx.doi.org/10.1016/j.amjcard.2021.07.040DOI Listing
August 2021

A Monolithic Solid-State Sodium-Sulfur Battery with Al-Doped NaZr(SiPO) Electrolyte.

ACS Appl Mater Interfaces 2021 Sep 31;13(36):42927-42934. Epub 2021 Aug 31.

School of Physical Science and Technology, Guangxi University, Nanning 530004, P. R. China.

The limit of the energy density and increasing security issues on sodium-ion batteries (SIBs) impede their further development. Solid-state sodium metal batteries are potential candidates to replace the present SIBs. However, low ionic conductivity and poor interface contact hinder their progress. In this work, the impact of Al doping on the crystalline structure and ionic transport in NaZr(SiPO) was studied by neutron powder diffraction. The ionic conductivity of NaZrAlSiPO achieves 4.43 × 10 S cm at 50 °C. The polarization voltage of the Na||Na symmetric battery is about 40 mV after cycling for more than 1600 h. Moreover, a solid-state sodium-sulfur battery with a monolithic structure was constructed to alleviate the interfacial resistance problems. Its specific discharge capacity can still keep 300 mA h g after 480 cycles at 300 mA g. The work provides a promising strategy to design solid-state sodium-sulfur batteries with high performances.
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http://dx.doi.org/10.1021/acsami.1c13000DOI Listing
September 2021

Priming COVID-19 salience increases prejudice and discriminatory intent against Asians and Hispanics.

Proc Natl Acad Sci U S A 2021 09;118(36)

Department of Sociology, University of California, Los Angeles, CA 90095.

Mounting reports in the media suggest that the COVID-19 pandemic has intensified prejudice and discrimination against racial/ethnic minorities, especially Asians. Existing research has focused on discrimination against Asians and is primarily based on self-reported incidents or nonrepresentative samples. We investigate the extent to which COVID-19 has fueled prejudice and discrimination against multiple racial/ethnic minority groups in the United States by examining nationally representative survey data with an embedded vignette experiment about roommate selection (collected in August 2020; = 5,000). We find that priming COVID-19 salience has an immediate, statistically significant impact: compared to the control group, respondents in the treatment group exhibited increased prejudice and discriminatory intent against East Asian, South Asian, and Hispanic hypothetical room-seekers. The treatment effect is more pronounced in increasing extreme negative attitudes toward the three minority groups than decreasing extreme positive attitudes toward them. This is partly due to the treatment increasing the proportion of respondents who perceive these minority groups as extremely culturally incompatible (Asians and Hispanics) and extremely irresponsible (Asians). Sociopolitical factors did not moderate the treatment effects on attitudes toward Asians, but prior social contact with Hispanics mitigated prejudices against them. These findings suggest that COVID-19-fueled prejudice and discrimination have not been limited to East Asians but are part of a broader phenomenon that has affected Asians generally and Hispanics as well.
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http://dx.doi.org/10.1073/pnas.2105125118DOI Listing
September 2021

Re-intervention rate in endovascular vs open surgical repair for abdominal aortic aneurysms.

Ann Med Surg (Lond) 2021 Sep 10;69:102703. Epub 2021 Aug 10.

Aswan University Hospitals, Egypt.

A best evidence topic has been constructed using a described protocol. The three-part question addressed was: In patients with Infrarenal abdominal aortic aneurysm (AAA), Does endovascular abdominal aortic repair (EVAR), AS compared to open surgical repair (OSR), has lower re-intervention rates? The outcomes assessed were the re-interventional rates in both techniques. The best evidence showed that the OSR has lower statistically significant difference rates in re-intervention rates than the EVAR.
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http://dx.doi.org/10.1016/j.amsu.2021.102703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379468PMC
September 2021

Kinesin superfamily proteins: roles in osteosarcoma.

Front Biosci (Landmark Ed) 2021 Aug;26(8):370-378

School of Basic Medicine, Gannan Medical University, 341000 Ganzhou, Jiangxi, China.

: Osteosarcoma is a common bone tumor with extremely high malignancy, occurring mostly in children and adolescents. At present, the survival rate of osteosarcomas has made progress in some aspects; however, this can only be regarded as a partial success because substantial progress has not been made in the last few decades. : The kinesin superfamily is a group of proteins that play regulatory roles in various metabolic processes and are closely related to tumor metastasis. Increasing evidence shows that kinesins play key roles in the occurrence and development of human cancer. : This review summarizes the roles of the kinesin superfamily proteins in osteosarcoma and related functions.
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http://dx.doi.org/10.52586/4950DOI Listing
August 2021

Functions of the Thyroid-Stimulating Hormone on Key Developmental Features Revealed in a Series of Zebrafish Dyshormonogenesis Models.

Cells 2021 Aug 4;10(8). Epub 2021 Aug 4.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.

The hypothalamic-pituitary-thyroid (HPT) axis regulates many critical features in vertebrates. Utilizing TALENs and CRISPR/Cas9 techniques, thyroid-stimulating hormone subunit beta a (), thyroglobulin (), and solute carrier family 16 member 2 () mutant zebrafish lines were generated. Among the three mutants, the earliest time point for the significantly altered T3 contents was observed in mutants, which resulted in the most severe defects, including typical defects such as the retardation of inflated anterior swimming bladder (aSB), proper formation of fin ray and posterior squamation (SP), the larval-to-juvenile transition (LTJT) process, juvenile growth retardation, and mating failure. In mutants, which are actually compensated with an alternative splicing form, growth retardation was observed in the juvenile stage without LTJT and reproductive defects. The evident goiter phenotype was only observed in - and mutants, but not in mutants. Other than goiters being observed, no other significant developmental defects were found in the mutants. Regarding the reproductive defects observed in mutants, the defective formation of the secondary sex characteristics (SSCs) was observed, while no obvious alterations during gonad development were found. Based on our analyses, zebrafish at the 6-12 mm standard length or 16-35 days post-fertilization (dpf) should be considered to be in their LTJT phase. Using a series of zebrafish dyshormonogenesis models, this study demonstrated that the TSH function is critical for the proper promotion of zebrafish LTJT and SSC formation. In addition, the elevation of TSH levels appears to be essential for goiter appearance in zebrafish.
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http://dx.doi.org/10.3390/cells10081984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391828PMC
August 2021

Effect and Safety of Kangfuyan Capsules () for Relieving Chronic Pelvic Pain: A Multicenter, Randomized, Controlled, Double-Blind, Parallel-Group Clinical Trial.

Chin J Integr Med 2021 Aug 25. Epub 2021 Aug 25.

China Association of Traditional Chinese Medicine, Beijing, 100101, China.

Objective: To evaluate the effect and safety of Kangfuyan Capsules () for treating pelvic inflammatory disease (PID) in patients with chronic pelvic pain (CPP) in a multicenter, randomized, controlled, double-blind, parallel-group clinical trial.

Methods: Totally, 240 PID patients with CPP were randomized into 2 groups using a computer generated random number at a 1:1 ratio from 10 hospitals in China between September 2014 and November 2015. Patients received either oral Kangfuyan Capsules or Gongyanping Capsules (, control); the regimen for both groups comprised 4 capsules (3 times daily) for 12 weeks, with follow-up visit 4 weeks after treatment. The visual analogue scale (VAS) scores, clinical responses, remarkable cure rates for each symptom, and quality of life scores were assessed at baseline, and after 1, 2, and 3 months. Adverse events were also recorded.

Results: The VAS scores were significantly lower (P<0.05), whereas the clinical responses, remarkable cure rates for lower abdominal pain, uterine tenderness, adnexal mass, and adnexal tenderness, and Health-related quality of life (EQ-5D) scores were higher in the Kangfuyan group than in the control group at 3 months (P<0.05). Common treatment-related adverse events included high hepatic enzyme levels, reduced hemoglobin levels, and elevated platelet counts, although all the adverse events were either mild or moderate in severity.

Conclusion: Compared with Gongyanping therapy, Kangfuyan therapy yielded markedly better analgesia effects for CPP caused by PID, with obvious long-term efficacy and good safety. (Registration No. ChiCTR190022732).
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http://dx.doi.org/10.1007/s11655-021-3490-7DOI Listing
August 2021

FNDC4 acts as an extracellular factor to promote the invasiveness of hepatocellular carcinoma partly via the PI3K/Akt signalling pathway.

Cancer Med 2021 Aug 21. Epub 2021 Aug 21.

Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University (Army Medical University, Chongqing, China.

FNDC4 is highly homologous to the exercise-associated myokine FNDC5/irisin, which is highly expressed and promotes the invasion and metastasis of HCC cells. However, the function of FNDC4 remains unknown. Here, we report that FNDC4, an extracellular factor, plays important roles in the invasion and metastasis of HCC. We found that high FNDC4 expression is associated with poor survival in HCC patients and FNDC4 promotes the migration and invasion of HCC cells. Mechanistically, we found that FNDC4 is related to the PI3K/Akt signalling pathway to a certain extent. Specifically, the extracellular domain of FNDC4 acts as an extracellular factor to promote Akt phosphorylation levels in this pathway. These findings reveal that FNDC4 promotes the invasion and metastasis of HCC partly via the PI3K/Akt signalling pathway.
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http://dx.doi.org/10.1002/cam4.4225DOI Listing
August 2021

The occurrence of per- and polyfluoroalkyl substances (PFASs) in fluoropolymer raw materials and products made in China.

J Environ Sci (China) 2021 Sep 12;107:77-86. Epub 2021 Feb 12.

Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Perfluorooctanoic acid (PFOA), its salts, and related compounds were listed as new persistent organic pollutants by the Stockholm Convention in 2019. In this study, the occurrence of residues of PFOA and other per- and polyfluoroalkyl substances (PFASs) in raw materials and fluoropolymer products from the Chinese fluoropolymer industries are reported for the first time. The PFOA concentrations in raw materials and fluoropolymer products were in the range of 6.7 to 1.1 × 10 ng/g, and
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http://dx.doi.org/10.1016/j.jes.2021.01.027DOI Listing
September 2021

Detecting Mycobacterium tuberculosis complex and rifampicin resistance via a new rapid multienzyme isothermal point mutation assay.

Anal Biochem 2021 Oct 16;630:114341. Epub 2021 Aug 16.

State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. Electronic address:

Simple, rapid, and accurate detection of the Mycobacterium tuberculosis complex (MTBC) and drug resistance is critical for improving patient care and decreasing the spread of tuberculosis. To this end, we have developed a new simple and rapid molecular method, which combines multienzyme isothermal rapid amplification and a lateral flow strip, to detect MTBC and simultaneously detect rifampin (RIF) resistance. Our findings showed that it has sufficient sensitivity and specificity for discriminating 118 MTBC strains from 51 non-tuberculosis mycobacteria strains and 11 of the most common respiratory tract bacteria. Further, compared to drug susceptibility testing, the assay has a sensitivity, specificity, and accuracy of 54.1%, 100.0%, and 75.2%, respectively, for detection of RIF resistance. Some of the advantages of this assay are that no special instrumentation is required, a constant low temperature of 39 °C is sufficient for the reaction, the turnaround time is less than 20 min from the start of the reaction to read out and the result can be seen with the naked eye and does not require specialized training. These characteristics of the new assay make it particularly useful for detecting MTBC and RIF resistance in resource-limited settings.
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http://dx.doi.org/10.1016/j.ab.2021.114341DOI Listing
October 2021

Growth of two-layer copolymer as the stationary phase with very high separation efficiency for separating peptides in capillary electrochromatography.

Electrophoresis 2021 Aug 19. Epub 2021 Aug 19.

Department of Chemistry, Inha University, Incheon, South Korea.

Open tubular CEC (OT-CEC) column with a very high separation efficiency was prepared for peptides separation. A pretreated silica-fused capillary was reacted with 3-(methacryloxy) propyltrimethoxysilane followed by vinylbenzyl chloride and divinylbenzene to produce first thin monolithic monolayer. The second copolymer layer was formed on thin monolithic monolayer of the capillary by reversible addition-fragmentation transfer polymerization of N-phenylacrylamide and styrene. The key parameters including buffer pH value and organic modifier were systematically evaluated to provide the optimal chromatographic condition. The resultant OT-CEC columns were validated by separating a synthetic mixture of peptides and cytochrome C tryptic digest in capillary electrochromatography. The number of theoretical plates as high as 2.4 million per column was achieved for synthetic mixture peptides. In addition, the fabricated OT-CEC column also resolved more than 18 high-efficiency digestion peptides from a mixture containing tryptic digest of cytochrome C. The column to column and inter- to intraday repeatabilities of OT-CEC column through RSD% were found better than 3.0%, exhibiting satisfactory stability and repeatability of the two-layer deposited OT-CEC column. The results reveal that the open tubular capillary column modified with two-layer copolymer shows the great prospect for the separation of proteins in capillary electrochromatography.
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http://dx.doi.org/10.1002/elps.202100136DOI Listing
August 2021

Association of Statin Treatment With Progression of Coronary Atherosclerotic Plaque Composition.

JAMA Cardiol 2021 Aug 18. Epub 2021 Aug 18.

Department of Pathology, CVPath Institute, Gaithersburg, Maryland.

Importance: The density of atherosclerotic plaque forms the basis for categorizing calcified and noncalcified morphology of plaques.

Objective: To assess whether alterations in plaque across a range of density measurements provide a more detailed understanding of atherosclerotic disease progression.

Design, Setting, And Participants: This cohort study enrolled 857 patients who underwent serial coronary computed tomography angiography 2 or more years apart and had quantitative measurements of coronary plaques throughout the entire coronary artery tree. The study was conducted from 2013 to 2016 at 13 sites in 7 countries.

Main Outcomes And Measures: The main outcome was progression of plaque composition of individual coronary plaques. Six plaque composition types were defined on a voxel-level basis according to the plaque attenuation (expressed in Hounsfield units [HU]): low attenuation (-30 to 75 HU), fibro-fatty (76-130 HU), fibrous (131-350 HU), low-density calcium (351-700 HU), high-density calcium (701-1000 HU), and 1K (>1000 HU). The progression rates of these 6 compositional plaque types were evaluated according to the interaction between statin use and baseline plaque volume, adjusted for risk factors and time interval between scans. Plaque progression was also examined based on baseline calcium density. Analysis was performed among lesions matched at baseline and follow-up. Data analyses were conducted from August 2019 through March 2020.

Results: In total, 2458 coronary lesions in 857 patients (mean [SD] age, 62.1 [8.7] years; 540 [63.0%] men; 548 [63.9%] received statin therapy) were included. Untreated coronary lesions increased in volume over time for all 6 compositional types. Statin therapy was associated with volume decreases in low-attenuation plaque (β, -0.02; 95% CI, -0.03 to -0.01; P = .001) and fibro-fatty plaque (β, -0.03; 95% CI, -0.04 to -0.02; P < .001) and greater progression of high-density calcium plaque (β, 0.02; 95% CI, 0.01-0.03; P < .001) and 1K plaque (β, 0.02; 95% CI, 0.01-0.03; P < .001). When analyses were restricted to lesions without low-attenuation plaque or fibro-fatty plaque at baseline, statin therapy was not associated with a change in overall calcified plaque volume (β, -0.03; 95% CI, -0.08 to 0.02; P = .24) but was associated with a transformation toward more dense calcium. Interaction analysis between baseline plaque volume and calcium density showed that more dense coronary calcium was associated with less plaque progression.

Conclusions And Relevance: The results suggest an association of statin use with greater rates of transformation of coronary atherosclerosis toward high-density calcium. A pattern of slower overall plaque progression was observed with increasing density. All findings support the concept of reduced atherosclerotic risk with increased densification of calcium.
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http://dx.doi.org/10.1001/jamacardio.2021.3055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374741PMC
August 2021

The Clinical Efficacy of Suture-Button Fixation and Trans-Syndesmotic Screw Fixation in the Treatment of Ankle Fracture Combined With Distal Tibiofibular Syndesmosis Injury: A Retrospective Study.

J Foot Ankle Surg 2021 Jul 20. Epub 2021 Jul 20.

Department of Orthopedics Trauma, Honghui Hospital Affiliated to School of Medicine, Xi'an Jiaotong University, Xi'an, P.R. China.

Twenty percent of ankle fractures present with concomitant syndesmosis injury which results in poor clinical outcomes. Surgical stabilization of the syndesmosis can be achieved with either trans-syndesmotic screws or a suture button device. The aim of this study is to investigate the clinical efficacy of suture button fixation and trans-syndesmotic screw fixation in the treatment of ankle fracture combined with distal tibiofibular syndesmosis injury. A retrospective analysis was conducted by enrolling 76 patients with ankle fractures combined with distal tibiofibular syndesmosis injury who were admitted in our trauma center from January 2018 to January 2019, including 34 cases of suture button group and 42 cases of the syndesmotic screw group with a mean follow-up period of 16 ± 7 (range 12-21) months. The demographic data included gender, age, injury mechanism, AO classification and the operation duration were recorded, the radiographic and clinical outcomes were determined by tibiofibular clear space, tibiofibular overlap distance, complications, and the Olerud-Molander Ankle Score at the last follow-up. All the indexes were compared between the 2 groups to discover the related statistical differences. With the numbers available, no significant difference could be detected in the surgical duration, tibiofibular clear space, tibiofibular overlap distance, total complication rate, and middle-term Olerud-Molander Ankle scores between the 2 groups. However, the suture button fixation group showed higher early stage Olerud-Molander Ankle scores (p = .027) and shorter full weightbearing time (p = .018) than that of syndesmotic screw fixation group. Considering the outcomes, we conclude that the suture button fixation not only shows equivalent efficacy to the traditional syndesmotic screw, but also has advantages of allowing early weightbearing, low requirements for routine removal.
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http://dx.doi.org/10.1053/j.jfas.2021.07.009DOI Listing
July 2021

Cardiac CIP protein regulates dystrophic cardiomyopathy.

Mol Ther 2021 Aug 14. Epub 2021 Aug 14.

Department of Cardiology, Boston Children's Hospital, Harvard Medical School, 320 Longwood Avenue, Boston, MA 02115, USA; Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA. Electronic address:

Heart failure is a leading cause of fatality in Duchenne muscular dystrophy (DMD) patients. Previously, we discovered that cardiac and skeletal-muscle-enriched CIP proteins play important roles in cardiac function. Here, we report that CIP, a striated muscle-specific protein, participates in the regulation of dystrophic cardiomyopathy. Using a mouse model of human DMD, we found that deletion of CIP leads to dilated cardiomyopathy and heart failure in young, non-syndromic mdx mice. Conversely, transgenic overexpression of CIP reduces pathological dystrophic cardiomyopathy in old, syndromic mdx mice. Genome-wide transcriptome analyses reveal that molecular pathways involving fibrogenesis and oxidative stress are affected in CIP-mediated dystrophic cardiomyopathy. Mechanistically, we found that CIP interacts with dystrophin and calcineurin (CnA) to suppress the CnA-Nuclear Factor of Activated T cells (NFAT) pathway, which results in decreased expression of Nox4, a key component of the oxidative stress pathway. Overexpression of Nox4 accelerates the development of dystrophic cardiomyopathy in mdx mice. Our study indicates CIP is a modifier of dystrophic cardiomyopathy and a potential therapeutic target for this devastating disease.
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http://dx.doi.org/10.1016/j.ymthe.2021.08.022DOI Listing
August 2021

Nitric oxide-releasing micelles with intelligent targeting for enhanced anti-tumor effect of cisplatin in hypoxia.

J Nanobiotechnology 2021 Aug 16;19(1):246. Epub 2021 Aug 16.

School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Background: Hypoxic tumor microenvironment (TME) promotes tumor metastasis and drug resistance, leading to low efficiency of cancer chemotherapy. The development of targeted agents or multi-target therapies regulating hypoxic microenvironment is an important approach to overcome drug resistance and metastasis.

Methods: In this study, chitosan oligosaccharide (COS)-coated and sialic acid (SA) receptor-targeted nano-micelles were prepared using film dispersion method to co-deliver cisplatin (CDDP) and nitric oxide (NO) (denoted as CTP/CDDP). In addition, we explored the mechanisms by which NO reversed CDDP resistance as well as enhanced anti-metastatic efficacy in hypoxic cancer cells.

Results: Because of the different affinities of COS and SA to phenylboronic acid (PBA) under different pH regimes, CTP/CDDP micelles with intelligent targeting property increased cellular uptake of CDDP and enhanced cytotoxicity to tumors, but reduced systemic toxicity to normal organs or tissues. In addition, CTP/CDDP showed stimulus-responsive release in TME. In terms of anti-tumor mechanism, CTP/CDDP reduced CDDP efflux and inhibited epithelial-mesenchymal transition (EMT) process of tumor by down-regulating hypoxia-inducible factor-1α (HIF-1α), glutathione (GSH), multidrug resistance-associated protein 2 (MRP2) and matrix metalloproteinase 9 (MMP9) expression, thus reversing drug resistance and metastasis of hypoxic tumor cells.

Conclusions: The designed micelles significantly enhanced anti-tumor effects both in vitro and in vivo. These results suggested that CTP/CDDP represented a promising strategy to treat resistance and metastatic tumors.
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http://dx.doi.org/10.1186/s12951-021-00989-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365946PMC
August 2021

Oxygen-Deficient β[email protected] Oxide Cathode for High-Rate and Long-Life Aqueous Zinc Ion Batteries.

Nanomicro Lett 2021 Aug 13;13(1):173. Epub 2021 Aug 13.

School of Advanced Materials, Peking University Shenzhen Graduate School, Shenzhen, 518055, People's Republic of China.

Recent years have witnessed a booming interest in grid-scale electrochemical energy storage, where much attention has been paid to the aqueous zinc ion batteries (AZIBs). Among various cathode materials for AZIBs, manganese oxides have risen to prominence due to their high energy density and low cost. However, sluggish reaction kinetics and poor cycling stability dictate against their practical application. Herein, we demonstrate the combined use of defect engineering and interfacial optimization that can simultaneously promote rate capability and cycling stability of MnO cathodes. β-MnO with abundant oxygen vacancies (V) and graphene oxide (GO) wrapping is synthesized, in which V in the bulk accelerate the charge/discharge kinetics while GO on the surfaces inhibits the Mn dissolution. This electrode shows a sustained reversible capacity of ~ 129.6 mAh g even after 2000 cycles at a current rate of 4C, outperforming the state-of-the-art MnO-based cathodes. The superior performance can be rationalized by the direct interaction between surface V and the GO coating layer, as well as the regulation of structural evolution of β-MnO during cycling. The combinatorial design scheme in this work offers a practical pathway for obtaining high-rate and long-life cathodes for AZIBs.
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http://dx.doi.org/10.1007/s40820-021-00691-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363675PMC
August 2021

Central Nervous System Cell-Derived Exosomes in Neurodegenerative Diseases.

Oxid Med Cell Longev 2021 13;2021:9965564. Epub 2021 Jul 13.

Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

Exosomes are a type of extracellular vesicles secreted by almost all kinds of mammalian cells that shuttle "cargo" from one cell to another, indicative of its role in cell-to-cell transportation. Interestingly, exosomes are known to undergo alterations or serve as a pathway in multiple diseases, including neurodegenerative diseases. In the central nervous system (CNS), exosomes originating from neurons or glia cells contribute to or inhibit the progression of CNS-related diseases in special ways. In lieu of this, the current study investigated the effect of CNS cell-derived exosomes on different neurodegenerative diseases.
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http://dx.doi.org/10.1155/2021/9965564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294976PMC
July 2021

Advanced Inorganic Nitride Nanomaterials for Renewable Energy: A Mini Review of Synthesis Methods.

Front Chem 2021 9;9:638216. Epub 2021 Jul 9.

Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), National and Local Joint Engineering Laboratory for New Petrochemical Materials and Fine Utilization of Resources, Key Laboratory of the Assembly and Application of Organic Functional Molecules of Hunan Province, Hunan Normal University, Changsha, China.

Inorganic nitride nanomaterials have attracted widespread attention for applications in renewable energy due to novel electrochemical activities and high chemical stabilities. For different renewable energy applications, there are many possibilities and uncertainties about the optimal nitride phases and nanostructures, which further promotes the exploration of controllable preparation of nitride nanomaterials. Moreover, unlike conventional nitrides with bulk or ceramic structures, the synthesis of nitride nanomaterials needs more accurate control to guarantee the target nanostructure along with the phase purity, which make the whole synthesis still a challenge to achieve. In this mini review, we mainly summarize the synthesis methods for inorganic nitride nanomaterials, including chemistry vapor deposition, self-propagation high-temperature synthesis, solid state metathesis reactions, solvothermal synthesis, . From the perspective of nanostructure, several novel nitrides, with nanostructures like nanoporous, two-dimensional, defects, ternary structures, and quantum dots, are showing unique properties and getting extensive attentions, recently. Prospects of future research in design and synthesis of functional inorganic nitrides are also discussed.
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http://dx.doi.org/10.3389/fchem.2021.638216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299337PMC
July 2021

A Honeycomb-Like Bismuth/Manganese Oxide Nanoparticle with Mutual Reinforcement of Internal and External Response for Triple-Negative Breast Cancer Targeted Therapy.

Adv Healthc Mater 2021 Jul 23:e2100518. Epub 2021 Jul 23.

The State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques, School of Materials Science and Technology, South China University of Technology, School of Physics, South China University of Technology, Guangzhou, Guangdong, 510640, China.

Triple-negative breast cancer (TNBC) exhibits aggressive behavior and high levels of metastasis owing to its complex heterogeneous structure and lack of specific receptors. Here, tumor cell membrane (CM)-coated bismuth/manganese oxide nanoparticles (NPs) with high indocyanine green (ICG) payload up to 50.6 wt% (mBMNI NPs) for targeted TNBC therapy are constructed. The extra-high drug load [email protected] O @MnO NPs (honey-comb like structure) are formed by Kirkendall effect and electrostatic attraction. After modified with CM, they can home into tumor sites precisely, where they respond to internal overexpressed glutathione (GSH), releasing Mn for chemodynamic therapy (CDT) with GSH depletion, while H O degrades into O enabling relief of tumor hypoxia. In response to external near-infrared irradiation, mBMNI NPs intelligently generate vigorous heat and single oxygen ( O ) for photothermal therapy (PTT) and photodynamic therapy (PDT) owing to high load. Importantly, O production and GSH consumption during the internal response reinforce external PDT, while the heat generated through PTT during the external response promotes internal CDT. The honeycomb-like structure with high ICG load and mutual reinforcement between internal and external response results in excellent therapeutic effects against TNBC.
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http://dx.doi.org/10.1002/adhm.202100518DOI Listing
July 2021

Association of serum apolipoprotein B with retinal neurovascular structural alterations in patients with type 2 diabetes: an optical coherence tomography angiography study.

Acta Diabetol 2021 Jul 22. Epub 2021 Jul 22.

Department of Ophthalmology, Shaanxi Provincial People's Hospital, Xi'an, 710068, China.

Purpose: To investigate the association of serum apoB concentrations with retinal neurovascular structural alterations in type 2 diabetes patients without clinically visible retinopathy.

Methods: Eyes with no clinically visible diabetic retinopathy (DR) from diabetic patients with or without dyslipidemia were included. Changes in retinal neural structures, including the ganglion cell layer (GCL) and peripapillary retinal nerve fiber layer (RNFL) thicknesses, and microvascular metrics, including macular vessel density (VD) and perfusion density (PD) of the superficial capillary plexus, were measured with optical coherence tomography angiography (OCTA). Correlations between inner retinal layer thickness and OCTA metrics were analyzed. The association of serum apoB and diabetic retinal neurovascular structures was identified with regression analysis.

Results: A total of 148 eyes in the diabetes group (n = 74) and dyslipidemia group (n = 74) were enrolled. GCL and RNFL thicknesses in patients in the dyslipidemia group were significantly thinner than those in the diabetes group (all p < 0.025). The total area of the VD and PD in the dyslipidemia group was also decreased compared to that of the diabetes group (p < 0.05) and was found to correlate with GCL and RNFL (all p < 0.01) thicknesses in all diabetic patients. Serum apoB levels were positively related to low-density lipoprotein (LDL) and total cholesterol (TC). Moreover, the average GCL thickness was significantly associated with serum apoB levels (all p < 0.05).

Conclusions: GCL and RNFL thinning was significantly correlated with decreased retinal blood flow in diabetic patients with dyslipidemia. Strictly controlling serum lipids, especially apoB levels, might be an effective strategy for DR treatment.
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http://dx.doi.org/10.1007/s00592-021-01775-xDOI Listing
July 2021

SRSF5 regulates alternative splicing of pre-mRNA through modulating SF1 binding.

RNA Biol 2021 Jul 22:1-19. Epub 2021 Jul 22.

Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Science, Northeast Forestry University, Harbin, China.

Abbreviations: ARF: alternative reading frame, that is, p14ARF, or CDKN2A (cyclin-dependent kinase inhibitor 2A); β-gal: β-galactosidase; CLIP-seq: crosslinking and immunoprecipitation-sequencing; : the cyclin D binding myb-like transcription factor 1; ESS/ESE: exonic splicing silencer/enhancer; Ex: exon; FBS: fetal bovine serum; Gluc: Gaussia luciferase; hnRNPs: heterogeneous nuclear ribonucleoproteins; In: intron; ISS/ISE: intronic splicing silencer/enhancer; PBS: phosphate-buffered saline; PCR: polymerase chain reaction; PSI: percent-splice-in; qPCR: quantitative real-time PCR; RIP: RNA immunoprecipitation; RNAseq: RNA sequencing; RT: reverse transcription; SF1: splicing factor 1; SR: serine/arginine-rich proteins; SRSF5: serine and arginine-rich splicing factor 5; TCGA: the cancer genome atlas; UCSC: University of California, Santa Cruz. WT: Wild type.
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http://dx.doi.org/10.1080/15476286.2021.1947644DOI Listing
July 2021

LncRNA NEAT1 Acts as an miR-148b-3p Sponge to Regulate ROCK1 Inhibition of Retinoblastoma Growth.

Cancer Manag Res 2021 12;13:5587-5597. Epub 2021 Jul 12.

Research Center of Ophthalmology, The First Hospital of Qiqihar, Affiliated Qiqihar Hospital, Southern Medical University, Qiqihar, Heilongjiang Province, People's Republic of China.

Background: It is reported that long non-coding RNA nuclear paraspeckle assembly transcript 1 (LncRNA NEAT1) is involved in the occurrence and development of various cancers. However, the detailed biological function and mechanism of LncRNA NEAT1 in retinoblastoma are still unclear. So we will explore the biological function and possible mechanism of LncRNA NEAT1 in retinoblastoma.

Materials And Methods: Quantitative real-time PCR (qRT-PCR) was used to detect LncRNA NEAT1 in retinoblastoma tissues and cell lines. Cell counting kit 8, Transwell and flow cytometry were applied to explore cell proliferation, invasion and apoptosis. The target miRNAs (miR) of LncRNA NEAT1 and miR and downstream target genes were predicted using Starbase3.0 software and confirmed by double luciferase reporting test and RNA binding protein immunoprecipitation (RIP). Western Blot was applied to explore ROCK1 in cells, and tumor allogeneic experiment was applied to study the role of LncRNA NEAT1 on tumor growth.

Results: It was found that LncRNA NEAT1 was up-regulated in retinoblastoma tissues, cells and serum, and the prognosis of patients with high expression of LNC RNA NEAT 1 was poor. Functional analysis showed that knocking down LncRNA NEAT1 could weaken proliferation and invasion, and accelerate apoptosis. Tumor allogeneic experiment showed that sh-NEAT1 injection can inhibit tumor growth. In addition, LncRNA NEAT1 inhibited proliferation and invasion, and promoted apoptosis through miR-148b-3p/ROCK1 axis.

Conclusion: LncRNA NEAT1 can mediate miR-148b-3p/ROCK1 axis to weaken the proliferation and invasion of retinoblastoma.
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http://dx.doi.org/10.2147/CMAR.S271326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285126PMC
July 2021

Changes in the Cytokine Profiles of Patients with Chronic Hepatitis B during Antiviral Therapy.

Biomed Environ Sci 2021 Jun;34(6):443-453

Department of Hepatology Division 2, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China;Department of Hepatology Division 2, Peking University Ditan Teaching Hospital, Beijing 100015, China.

Objective: To investigate the changes in the cytokine profiles of chronic hepatitis B (CHB) patients undergoing antiviral treatment.

Methods: Hepatitis B e antigen (HBeAg)-positive patients were treated with Pegylated interferon (PEG-IFN) and entecavir (ETV). Clinical biochemistry and cytokines were detected at baseline and every 3 months.

Results: In all, 200 patients completed 48 weeks of treatment, 100 in the PEG-IFN group and 100 in the ETV group. During 3-6 months of treatment, compared with baseline, the PEG-IFN group showed a significant decrease in interferon-gamma (IFN-γ), interleukin-17A (IL-17A), interleukin-6(IL-6), interleukin-10(IL-10), and transforming growth factor beta (TGF-β) ( < 0.001) and a significant increase in interferon-alpha 2(IFN-α2) ( < 0.001). In the ETV group, IL-10 and TGF-β1 decreased significantly ( < 0.001). After 3 months, the levels of IFN-α2, IL-17A, and tumor necrosis factor-alpha(TNF-α) in the PEG-IFN group were significantly higher than those in the ETV group ( < 0.01). The levels of IL-6 and TGF-β3 were significantly lower than those in the ETV group ( < 0.01). After 6 months, the levels of IFN-α2, IFN-γ, and TNF-α in the PEG-IFN group were significantly higher than those in the ETV group ( < 0.01), while the levels of IL-6 and TGF-β3 were significantly lower than those in the ETV group ( < 0.01). Compared with ETV, PEG-IFN had higher HBeAg and HBsAg disappearance rates.

Conclusion: During antiviral therapy, a change in the cytokine profile occurred; in the aspect of immune control and functional cure, PEG-IFN was significantly better than ETV.
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http://dx.doi.org/10.3967/bes2021.061DOI Listing
June 2021
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