Publications by authors named "Yao Liu"

1,326 Publications

  • Page 1 of 1

Connexin 43 overexpression induces lung cancer angiogenesis following phosphorylation at Ser279 in its C-terminus.

Oncol Lett 2022 Sep 5;24(3):293. Epub 2022 Jul 5.

Department of Cardio-Thoracic Surgery, Shenzhen University General Hospital, Shenzhen, Guangdong 518055, P.R. China.

Blocking angiogenesis can inhibit tumor growth and metastasis. However, the mechanism underlying regulation of lung cancer angiogenesis remains unclear. The gap junction protein connexin 43 (Cx43) is implicated in angiogenesis. The aim of the present study was to determine the role of Cx43 in angiogenesis and its signaling pathways. Human pulmonary microvascular endothelial cells were transfected with Cx43-targeting siRNA or Cx43-overexpressing recombinant plasmid vector. Reverse transcription-quantitative polymerase chain reaction and western blotting were performed to determine Cx43, zonula occludens-1 (ZO-1), E-cadherin, β-catenin, von Willebrand factor (vWF), and plasminogen activator inhibitor-1 (PAI-1) mRNA and protein expression levels, respectively. Tyr265, Ser279, Ser368, and Ser373 phosphorylation levels in the C-terminus of Cx43 and intracellular and membranal Cx43 contents were determined using western blotting. Additionally, immunofluorescence, tube formation, Cell Counting Kit-8, and Transwell migration assays were performed. The results revealed that compared with that in the control samples, Cx43, ZO-1, E-cadherin, β-catenin, vWF, and PAI-1 mRNA and protein expression were significantly increased in the Cx43 overexpression group and significantly decreased in the Cx43-knockdown group. Moreover, the phosphorylation level of Ser279 as well as cell proliferation and migration rates were markedly increased in the Cx43 overexpression group, and tube formation revealed that the potential of angiogenesis was also increased. Conversely, in the Cx43-knockdown group, the phosphorylation level of Ser279 and cell proliferation and migration rates were reduced, and the potential of angiogenesis was greatly impaired. Under Cx43 overexpression, membranal Cx43 content was significantly increased, whereas under Cx43 knockdown, it was significantly reduced. Therefore, Cx43 overexpression could induce pulmonary angiogenesis by promoting cell proliferation and migration and activating ZO-1, E-cadherin, β-catenin, vWF, and PAI-1. This may be achieved by promoting phosphorylation and activation of the intracellular signal site Ser279 at the C-terminus of Cx43.
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http://dx.doi.org/10.3892/ol.2022.13413DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353244PMC
September 2022

Debaryomyces nepalensis reduces fungal decay by affecting the postharvest microbiome during jujube storage.

Int J Food Microbiol 2022 Aug 1;379:109866. Epub 2022 Aug 1.

College of Food Science, Southwest University, Chongqing 400715, PR China; Research Center of Food Storage & Logistics, Southwest University, Chongqing 400715, PR China. Electronic address:

Microbial antagonists are effective and environmentally friendly in controlling postharvest diseases of fruit. The present study investigated the influence of D. nepalensis on epiphytic microbiome and postharvest decay of jujube. Results showed that D. nepalensis notably reduced fungal decay, maintained the fruit firmness and delayed discoloration. The epiphytic microbiome revealed that D. nepalensis changed the fungal communities, but few influence on bacterial communities were observed. D. nepalensis, as the dominant population in the treatment group, decreased the abundance of pathogenic fungi of Alternaria, Penicillium, Fusarium and Botrytis, while increased the beneficial bacteria of Pantoea. The canonical correspondence analysis revealed that Debaryomyces was negatively correlated with the decay rate, whereas Penicillium, Acremonium, Rhodosporidiobolus and Hansfordia were positively correlated. In conclusion, D. nepalensis altered the successional process of fungal and bacterial communities to reduce the decay rate of jujube during storage.
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http://dx.doi.org/10.1016/j.ijfoodmicro.2022.109866DOI Listing
August 2022

What matters the most in curbing early COVID-19 mortality? A cross-country necessary condition analysis.

Public Adm 2022 Jul 17. Epub 2022 Jul 17.

School of Public Policy and Administration Xi'an Jiaotong University Xi'an China.

COVID-19 represents a turbulent problem: a volatile, uncertain, complex, and ambiguous crisis, in which bounded-rational policymakers may not be able to do everything right, but must do critical things right in order to reduce the death toll. This study conceptualizes these critical things as necessary conditions (NCs) that must be absent to prevent high early mortality from occurring. We articulate a policy-institution-demography framework that includes seven factors as NC candidates for high early COVID-19 mortality. Using necessary condition analysis (NCA), this study pinpoints high levels of a delayed first response, political decentralization, elderly populations, and urbanization as four NCs that have inflicted high early COVID-19 mortality across 110 countries. The results highlight the critical role of agility as a key dimension of robust governance solutions-a swift early public-health response as a malleable policy action-in curbing early COVID-19 deaths, particularly for politically decentralized and highly urbanized countries with aging populations.
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http://dx.doi.org/10.1111/padm.12873DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350176PMC
July 2022

Efficacy and Safety of Coenzyme Q10 Supplementation in the Treatment of Polycystic Ovary Syndrome: a Systematic Review and Meta-analysis.

Reprod Sci 2022 Aug 8. Epub 2022 Aug 8.

Department of Cardiology, The First Affiliated Hospital of University of South China, Hunan Province, Hengyang, China.

The aim of this study is to evaluate the efficacy and safety of coenzyme Q10 supplementation in the treatment of polycystic ovary syndrome (PCOS). We first searched PubMed, Wanfang Data, CNKI, Embase, ClinicalTrial.gov, and other databases. The retrieval time from the establishment of the database to January 2021. We collected relevant randomized controlled trials (RCTs) about coenzyme Q10 in the treatment of PCOS. Risk of bias assessment and meta-analysis of RCTs were performed using RevMan 5.0 software. This systematic review and meta-analysis include a total of 9 RCTs involving 1021 patients. The results show that the addition of coenzyme Q10 may improve insulin resistance (HOMA-IR (WMD - 0.67 [- 0.87, - 0.48], P < 0.00001); fasting insulin (WMD - 1.75 [- 2.65, - 0.84], P = 0.0002); fasting plasma glucose (WMD - 5.20 [- 8.86, - 1.54], P = 0.005)), improve sex hormone levels (FSH (SMD - 0.45 [0.11, 0.78], P = 0.009); testosterone (SMD - 0.28 [- 0.49, - 0.06], P = 0.01)), and improve blood lipids (triglycerides (SMD - 0.49 [- 0.89, - 0.09], P = 0.02); total cholesterol (SMD - 0.35 [- 0.56, - 0.14], P = 0.001); LDL-C (SMD - 0.22 [- 0.43, - 0.01], P = 0.04); HDL-C (SMD 0.22 [0.01, 0.43], P = 0.04)). Only one RCT reported adverse events, and they found that patients had no adverse effects or symptoms following supplementation. Based on the current evidence, it could be considered that the addition of CoQ10 is a safe therapy to improve PCOS by improving insulin resistance (reduce HOMA-IR, FINS, FPG), increasing sex hormone levels (increase FSH, reduce testosterone), and improving blood lipids (reduce TG, TC, LDL-C, and increased HDL-C).
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http://dx.doi.org/10.1007/s43032-022-01038-2DOI Listing
August 2022

Digital technology for orthognathic surgery training promotion: a randomized comparative study.

PeerJ 2022 2;10:e13810. Epub 2022 Aug 2.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Oral & Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Background: This study aims to investigate whether a systematic digital training system can improve the learning efficiency of residents in the first-year orthognathic surgery training course and evaluate its effectiveness in teaching orthognathic surgery.

Methods: A digital training system was applied, and a comparative research approach was adopted. 24 first-year orthognathic surgery residents participated in the experiment as part of their professional skill training. The Experimental group was required to use a digital training system, and the Control group was trained in lectures without digital technologies. Three indicators, including theoretical knowledge and clinical operation, were assessed in tests, and evaluations from instructors were analyzed to evaluate learning efficiency.

Results: The results showed that the scores in theoretical tests, practical operations, and teacher evaluations, the Experimental groups were all higher than the Control group ( = 0.002 for anatomy,  = 0.000 for operation theory) after using digital technology, except for the understanding of complications ( = 0.771). In addition, the questionnaire survey results showed that the study interest ( = 0.001), self-confidence ( = 0.001), satisfaction ( = 0.002), and academic performance ( = 0.001) of the residents of the Experimental group were higher than those of the Control group.

Conclusions: The outcomes indicated that the digital training system could benefit orthognathic residents' learning efficiency, and learning interest and teaching satisfaction will also improve.
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http://dx.doi.org/10.7717/peerj.13810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354792PMC
August 2022

Simultaneous measurement of six biomarkers of dichlorvos in blood by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap mass spectrometry.

J Chromatogr B Analyt Technol Biomed Life Sci 2022 Jul 29;1208:123381. Epub 2022 Jul 29.

School of Forensic Medicine, Shanxi Medical University and and Key Laboratory of Forensic Toxicology of Ministry of Public Security, Jinzhong 030600, Shanxi, China; Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China. Electronic address:

A protocol was developed, via a data-dependent high-resolution tandem mass spectrometry (ddHRMS/MS), to detect six biomarkers of dichlorvos in its metabolites and blood adducts of butyrylcholinesterase and albumin without using standard synthetic peptides. Firstly, the adducts of dimethoxy phosphonate (DMP-BChE) and the aged adducts of methoxy phosphonate (MxP-BChE) were isoloated by immunomagnetic separation (IMS), and then digested to DMP-nonapeptide and MxP-nonapeptide by pepsin. The dichlorvos and its metabolites (Trimethyl phosphate, Dimethyl phosphate) were analyzed in the supernatant of IMS treatment after protein precipitation. The precipitate was digested by pronase to phosphorylated tyrosine (DMP-Tyr), which were quantified by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap mass spectrometry (UPLC-MS). The linearity of detector response of all biomarkers was studied in their respective ranges, and the correlation coefficients (R) were all greater than 0.9981. The limits of detection (LOD) and limits of quantification (LOQ) were 0.2-10 ng/mL and 0.5-20 ng/mL, respectively. The recoveries of the six biomarkers were 78.6-110.0 %, matrix effect were 64.1-106.8 %. Inter- and intra-assay precision had coefficients of variation of ≤13.2 % and ≤9.7 %, respectively. In addition, MxP- BChE, DMP-Tyr, TMP, DMP and DDVP were detected in the blood of three cases who died from DDVP poisoning, but DMP- BChE was not detected in all of them, which may be caused by the instability of DMP- BChE and its easy aging to form MxP-BCHE.
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http://dx.doi.org/10.1016/j.jchromb.2022.123381DOI Listing
July 2022

Exocrine pancreas regeneration modifies original pancreas to alleviate diabetes in mouse models.

Sci Transl Med 2022 Aug 3;14(656):eabg9170. Epub 2022 Aug 3.

Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, South China Center of Craniofacial Stem Cell Research, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.

Diabetes is a major public health issue because of its widely epidemic nature and lack of cure. Here, we show that pancreas-derived mesenchymal stem cells (PMSCs) are capable of regenerating exocrine pancreas when implanted into the kidney capsule of mice with streptozotocin (STZ)-induced diabetes. Mechanistically, we found that the regenerated exocrine pancreas elevated interleukin-6 (IL-6) in PMSC implants, which transiently activated tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) to inhibit IL-17, thereby rescuing damaged exocrine pancreas and islet β cells. In addition, we used knockout mouse models to show that global lack of IL-6, TNF-α, or IFN-γ resulted in increased severity of STZ-induced diabetes and resistance to PMSC implantation therapy, confirming the roles of these factors in safeguarding pancreatic β cells. Furthermore, removal of the kidney capsule PMSC implants at 28 days after implantation did not affect the PMSC-initiated therapeutic effect on diabetic mice. This study reveals a previously unknown role of exocrine pancreas regeneration in safeguarding β cells and demonstrates a "soil-rescues-seed" strategy for type 1 diabetes therapy.
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http://dx.doi.org/10.1126/scitranslmed.abg9170DOI Listing
August 2022

Low-Intensity Focused Ultrasound Alleviates Chronic Neuropathic Pain-Induced Allodynia by Inhibiting Neuroplasticity in the Anterior Cingulate Cortex.

Neural Plast 2022 23;2022:6472475. Epub 2022 Jul 23.

School of Rehabilitation, Kunming Medical University, Kunming, 650500 Yunnan Province, China.

Low-intensity focused ultrasound (LIFU) is a potential noninvasive method to alleviate allodynia by modulating the central nervous system. However, the underlying analgesic mechanisms remain unexplored. Here, we assessed how LIFU at the anterior cingulate cortex (ACC) affects behavior response and central plasticity resulting from chronic constrictive injury (CCI). The safety of LIFU stimulation was assessed by hematoxylin and eosin (H&E) and Fluoro-Jade C (FJC) staining. A 21-day ultrasound exposure therapy was conducted from day 91 after CCI surgery in mice. We assessed the 50% mechanical withdrawal threshold (MWT) using Von Frey filaments (VFFs). The expression levels of microtubule-associated protein 2 (MAP2), growth-associated protein 43 (GAP43), and tau were determined via western blotting (WB) and immunofluorescence (IF) staining to evaluate the central plasticity in ACC. The regions of ACC were activated effectively and safely by LIFU stimulation, which significantly increased the number of c-fos-positive cells ( < 0.05) with no bleeding, coagulative necrosis, and neuronal loss. Under chronic neuropathic pain- (CNP-) induced allodynia, MWT decreased significantly ( < 0.05), and overexpression of MAP2, GAP43, and tau was also observed. After 3 weeks of treatment, significant increases in MWT were found in the CCI+LIFU group compared with the CCI group ( < 0.05). WB and IF staining both demonstrated a significant reduction in the expression levels of MAP2, GAP43, and tau ( < 0.05). LIFU treatment on ACC can effectively attenuate CNP-evoked mechanical sensitivity to pain and reverse aberrant central plasticity.
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http://dx.doi.org/10.1155/2022/6472475DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338851PMC
August 2022

Hepatic epithelioid angiomyolipoma mimicking hepatocellular carcinoma on MR and F-FDG PET/CT imaging: A case report and literature review.

Hell J Nucl Med 2022 Aug 3. Epub 2022 Aug 3.

Department of Nuclear Medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310000, China.

Hepatic epithelioid angiomyolipoma (HEAML) is a rare hepatic mesenchymal tumor with malignant potential. Unlike hepatic angiomyolipoma, HEAML is devoid of adipocytes. Thus, it is easy to be misdiagnosed as other tumors of liver, especially hepatocellular carcinoma (HCC) on preoperative imaging examinations. Herein, we present a case of HEAML mimicking HCC on magnetic resonance (MR) and fluorine-18-fluorodeoxyglucose positron emission tomography/computed tomography (F-FDG PET/CT) in a 50-year-old female. After the primary diagnosis of HCC, the patient underwent a laparoscopic resection. The histopathology and immunohistochemical staining helped to reach the final diagnosis of HEAML. The important sign of central vessel can be seen on MRI in this case. The average apparent diffusion coefficient (ADC) value of the HEAML is 0.86±0.13x10mm/s, which is lower than that of 0.97±0.02?10mm/s of the normal liver. Previous literature on F-FDG PET/CT imaging of HEAML is limited to only 2 cases. The F-FDG PET/CT images of HEAML with high F-FDG accumulation with a maximum standardized uptake value (SUVmax) of 8.88 and extensive necrosis are presented, indicating its malignant potential. This case aims to improve the ability of differential diagnosis from HCC on multimodal imaging, and provides values for further F-FDG PET/CT related studies.
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http://dx.doi.org/10.1967/s002449912480DOI Listing
August 2022

Drainage volume on postoperative day one to predict clinically relevant postoperative pancreatic fistula following distal pancreatectomy.

BMC Surg 2022 Aug 1;22(1):297. Epub 2022 Aug 1.

Department of Nuclear Medicine, Second Affiliated Hospital of Chengdu Medical College (China National Nuclear Corporation 416 Hospital), Chengdu, Sichuan, 610000, People's Republic of China.

Background: The purpose of this study was to determine how the drain fluid volume on the first day after surgery (DFV 1) can be used to predict clinically relevant post-operative pancreatic fistula following distal pancreatectomy (DP).

Method: A retrospective analysis of 175 patients who underwent distal pancreatectomy in hepatobiliary surgery at Chengdu 363 Hospital (China) from January 2015 to January 2021 has been performed. Depending on the presence of pancreatic fistula, all patients were divided into two groups: POPF and non-POPF. The clinical factors were analyzed using SPSS 17.0 and Medcalc software. In order to assess the effectiveness of DFV 1 in predicting POPF after surgery, ROC curves were used to calculate its cut-off point,, which yielded sensitivity and negative predictive value of 100% for excluding POPF.

Result: Of the 175 patients who underwent distal pancreatectomy, the incidence of overall pancreatic fistula was 36%, but the rate of clinically significant (grade B and C) fistula, as defined by the International Study Group on Pancreatic Fistula, 30 was only 17.1% (28 grade B and 2 grade C fistula). The results from univariate and multivariate logistic regression analysis showed that drain fluid volume on the first postoperative day (OR = 0.95, P = 0.03), drainage fluid amylase level on POD1 (OR = 0.99, P = 0.01) and the preoperative ALT level (OR = 0.73, P = 0.02) were independent risk factors associated with CR-POPF. Receiver operating characteristic (ROC) curve analysis revealed that a drainage volume of 156 mL within 24 h and an amylase greater than 3219.2 U/L on the first postoperative day were the optimal thresholds associated with complications.

Conclusion: After distal pancreatectomy, the drainage volume on the first postoperative day can predict the presence of a clinically relevant pancreatic fistula.
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http://dx.doi.org/10.1186/s12893-022-01748-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341036PMC
August 2022

Structural diversity and bioactivity of polysaccharides from medicinal mushroom Phellinus spp.: A review.

Food Chem 2022 Jul 18;397:133731. Epub 2022 Jul 18.

State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. Electronic address:

Phellinus spp., an important medicinal fungus mushroom extensively cultivated and consumed in East Asia for over 2000 years, is traditionally considered a precious food supplement and medicinal ingredient. Published studies showed that the polysaccharides are major bioactive macromolecules from Phellinus spp. (PPs) with multiple health-promoting effects, including immunomodulatory, anti-cancer, anti-inflammatory, hepatoprotective, hypoglycemic, hypolipidemic, antioxidant, and other bioactivities. Although the polysaccharides extracted from the fruiting body, mycelium, and fermentation broth of Phellinus spp. have been extensively studied for the extraction and purification methods, structural characteristics, and pharmacological activities, the knowledge for their structures and bioactivity relationship, toxicologic effects, and pharmacokinetic profile is limited. This review systematically summarizes the recent progress in the isolation and purification, chemical structures, bioactivities, and the underlying mechanisms of PPs. Information from this review provides insights into the further development of polysaccharides from PPs as therapeutic agents and functional foods.
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http://dx.doi.org/10.1016/j.foodchem.2022.133731DOI Listing
July 2022

Impact of alcohol drinking and tobacco smoking on the drug-resistance of newly diagnosed tuberculosis: a retrospective cohort study in Shandong, China, during 2004-2020.

BMJ Open 2022 07 28;12(7):e059149. Epub 2022 Jul 28.

Department of Respiratory and Critical Care Medicine, Shandong Provincial Hospital, Jinan, Shandong, China

Objectives: To investigate the independent and collective impact of alcohol drinking and tobacco smoking on the drug-resistance of newly diagnosed tuberculosis (TB).

Design: This was a retrospective cohort study.

Setting: Shandong, China.

Participants: Patients with newly diagnosed TB from 1 January 2004 to 31 December 2020 were collected. Exclusive criteria: retreated cases; extrapulmonary tuberculosis; without information on drug susceptibility testing results, smoking or drinking habits; bacteriological identification as .

Primary And Secondary Outcome Measures: Patients were classified into four groups including smokers only (G), drinker only (G), smoker +drinker (G), non-smoker +non-drinker group (G). We described the drug-resistant profiles, clinical factors and calculated the ORs of different drug-resistance among G, G, G, compared with G through univariate and multivariate logistics regression models.

Results: Of the 7996 TB cases enrolled, the proportions of G, G, G and G were 8.25%, 3.89%, 16.46% and 71.40%, respectively. The rates of drug-resistant (DR)-TB, mono-resistant TB, multidrug resistant (MDR)-TB, polydrug resistant TB in G G, G and G were 19.24%/16.4%/17.33%/19.08%, 11.52%/8.68%/10.94%/11.63%, 3.03%/2.57%/2.96%/3.66% and 4.70%/4.82%/3.34%/ 4.08%, respectively. G had a higher risk of MDR1: isoniazid +rifampin (adjusted OR (aOR)=1.91, 95% CI: 1.036 to 3.532), but had a lower risk of DR-TB (aOR=0.84, 95% CI: 0.71 to 0.99), rifampin-related resistance (aOR=0.68, 95% CI: 0.49 to 0.93), streptomycin-related resistance (aOR=0.82, 95% CI: 0.68 to 0.99), ethambutol-related resistance (aOR=0.57, 95% CI: 0.34 to 0.95), MDR3: isoniazid +rifampin+streptomycin (aOR=0.41, 95% CI: 0.19 to 0.85), any isoniazid +streptomycin resistance (aOR=0.85, 95% CI: 0.71 to 1.00). However, there were no significant differences between G and G, G and G in all drug-resistant subtypes. Those patients with cavity had a higher risk of DR-TB among G (OR=1.35, 95% CI: 1.01 to 1.81).

Conclusion: Although we did not found an independent impact of alcohol drinking or tobacco smoking on TB drug-resistance, respectively, these two habits had a combined effect on TB drug-resistance.
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http://dx.doi.org/10.1136/bmjopen-2021-059149DOI Listing
July 2022

A Rapid and Efficient Strategy for Quality Control of Clinopodii herba Encompassing Optimized Ultrasound-Assisted Extraction Coupled with Sensitive Variable Wavelength Detection.

Molecules 2022 Jul 10;27(14). Epub 2022 Jul 10.

Anhui College of Traditional Chinese Medicine, Wuhu 241000, China.

Clinopodii herba is a folk herbal medicine for treatments of hemorrhagic disorders. However, there is not even a quantitative standard for clinopodii herba deposited in the Chinese Pharmacopoeia. The development of a strategy for rapid and efficient extraction and simultaneous detection of multiple components in clinopodii herba is therefore of great value for its quality evaluation. Here, a variable wavelength strategy was firstly applied to quantity multiple components by segmental monitoring by UHPLC with diode array detector following ultrasound-assisted extraction. The parameters of ultrasound-assisted extraction were optimized using single factor optimization experiments and response surface methodology by a Box-Behnken design combined with overall desirability. Subsequently, a rapid, efficient, and sensitive method was applied for simultaneous determination of eleven compounds, which represented the major and main types of components in clinopodii herba. Moreover, the performance of the validated method was successfully applied for the quality control of various batches of clinopodii herba and provided sufficient supporting data for the optimum harvest time. The Box-Behnken-optimized ultrasound-assisted extraction coupled with variable wavelength detection strategy established in this work not only improves the quality control of clinopodii herba, but also serves as a powerful approach that can be extended to quality evaluation of other traditional Chinese medicines.
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http://dx.doi.org/10.3390/molecules27144418DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316769PMC
July 2022

Eco-Friendly Extraction, Structure, and Gel Properties of ι-Carrageenan Extracted Using Ca(OH).

Mar Drugs 2022 Jun 27;20(7). Epub 2022 Jun 27.

College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.

An eco-friendly method for ι-carrageenan extraction from seaweed through boiling and using a low concentration of Ca(OH) is reported. Compared to the traditional method of ι-carrageenan extraction using NaOH, the reported method using Ca(OH) had the advantages of using 93.3% less alkali and 86.8% less water, having a 25.0% shorter total extraction time, a 17.6% higher yield, and a 43.3% higher gel strength of the product. In addition, we evaluated the gel properties and structures of ι-carrageenan products extracted by Ca(OH) (Ca-IC) and NaOH (Na-IC). The Fourier transform infrared spectroscopy results showed that the structures of Ca-IC and Na-IC did not change remarkably. The results of the thermogravimetric analysis and differential scanning calorimetry showed that Ca-IC had the same thermal stability as Na-IC. The results of the textural analysis showed that Ca-IC had a higher hardness and better chewiness compared to Na-IC. Rheological results indicated that Ca-IC and Na-IC exhibited shear-thinning and non-Newtonian fluid properties, whereas the viscosity of Ca-IC was less than that of Na-IC. In conclusion, this new method of ι-carrageenan extraction using Ca-IC is markedly better and yields higher quality carrageenan than the conventional method of using Na-IC.
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http://dx.doi.org/10.3390/md20070419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322172PMC
June 2022

In Situ Synthesis of Metal-Containing ZSM-5 and Its Catalytic Performance in Aromatization of Methanol.

ACS Omega 2022 Jul 10;7(28):24241-24248. Epub 2022 Jul 10.

State Key Laboratory of Chemical Resources Engineering, Beijing University of Chemical Technology, Beijing 100029, China.

Metal-containing ZSM-5 zeolites, denoted as M/ZSM-5 (M = Ga, Zn, and La), were prepared by an in situ synthetic method. The structural, textural, and surface acid properties of M/ZSM-5 were characterized by XRD, N adsorption-desorption, NH-TPD, and FT-IR. The catalyst pore size and the distribution of strong acid and weak acid were affected by the introduction of metal. Considering the methanol-to-aromatics model reaction, the catalytic performance of these samples was investigated in a fixed bed reactor under the conditions of 450 °C and a liquid weight hourly space velocity (WHSV) of 5 h. The results indicated that the Zn/ZSM-5 molecular sieve was most beneficial to aromatization; the relationship between the change of B/L caused by Zn content and aromatics selectivity was further studied. When the B/L value decreased, the selectivity of aromatics showed an initial increase and then a decrease. The B/L value was optimal, and the aromatics yield was the highest when the Zn loading was 0.35%.
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http://dx.doi.org/10.1021/acsomega.2c01442DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301955PMC
July 2022

Nanopore Identification of Alditol Epimers and Their Application in Rapid Analysis of Alditol-Containing Drinks and Healthcare Products.

J Am Chem Soc 2022 Aug 22;144(30):13717-13728. Epub 2022 Jul 22.

State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Alditols, which have a sweet taste but produce much lower calories than natural sugars, are widely used as artificial sweeteners. Alditols are the reduced forms of monosaccharide aldoses, and different alditols are diastereomers or epimers of each other and direct and rapid identification by conventional methods is difficult. Nanopores, which are emerging single-molecule sensors with exceptional resolution when engineered appropriately, are useful for the recognition of diastereomers and epimers. In this work, direct distinguishing of alditols corresponding to all 15 monosaccharide aldoses was achieved by a boronic acid-appended hetero-octameric porin A (MspA) nanopore (MspA-PBA). Thirteen alditols including glycerol, erythritol, threitol, adonitol, arabitol, xylitol, mannitol, sorbitol, allitol, dulcitol, iditol, talitol, and gulitol (l-sorbitol) could be fully distinguished, and their sensing features constitute a complete nanopore alditol database. To automate event classification, a custom machine-learning algorithm was developed and delivered a 99.9% validation accuracy. This strategy was also used to identify alditol components in commercially available "zero-sugar" drinks and healthcare products, suggesting their use in rapid and sensitive quality control for the food and medical industry.
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http://dx.doi.org/10.1021/jacs.2c04595DOI Listing
August 2022

Novel lncRNAs with diagnostic or prognostic value screened out from breast cancer via bioinformatics analyses.

PeerJ 2022 14;10:e13641. Epub 2022 Jul 14.

Longhua Innovation Institute for Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong, P.R. China.

Background: Recent studies have shown that long non-coding RNAs (lncRNAs) may play key regulatory roles in many malignant tumors. This study investigated the use of novel lncRNA biomarkers in the diagnosis and prognosis of breast cancer.

Materials And Methods: The database subsets of The Cancer Genome Atlas (TCGA) by RNA-seq for comparing analysis of tissue samples between breast cancer and normal control groups were downloaded. Additionally, anticoagulant peripheral blood samples were collected and used in this cohort study. The extracellular vesicles (EVs) from the plasma were extracted and sequenced, then analyzed to determine the expressive profiles of the lncRNAs, and the cancer-related differentially expressed lncRNAs were screened out. The expressive profiles and associated downstream-mRNAs were assessed using bioinformatics (such as weighted correlation network analysis (WGCNA), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genome (KEGG) enrichments, Receiver-Operating Characteristic (ROC) curve and survival analysis, ) to investigate the diagnostic and prognostic values of these EV lncRNAs and their effectors.

Results: In this study, 41 breast cancer-related lncRNAs were screen out from two datasets of tissue and fresh collected plasma samples of breast cancer via the transcriptomic and bioinformatics techniques. A total of 19 gene modules were identified with WGCNA analysis, of which five modules were significantly correlated with the clinical stage of breast cancer, including 28 lncRNA candidates. The ROC curves of these lncRNAs revealed that the area under the curve (AUC) of all candidates were great than 70%. However, eight lncRNAs had an AUC >70%, indicating that the combined one has a good diagnostic value. In addition, the results of survival analysis suggested that two lncRNAs with low expressive levels may indicate the poor prognosis of breast cancer. By tissue sample verification, C15orf54, AL157935.1, LINC01117, and SNHG3 were determined to have good diagnostic ability in breast cancer lesions, however, there was no significant difference in the plasma EVs of patients. Moreover, survival analysis data also showed that AL355974.2 may serve as an independent prognostic factor and as a protective factor.

Conclusion: A total of five lncRNAs found in this study could be developed as biomarkers for breast cancer patients, including four diagnostic markers (C15orf54, AL157935.1, LINC01117, and SNHG3) and a potential prognostic marker (AL355974.2).
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http://dx.doi.org/10.7717/peerj.13641DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288825PMC
July 2022

Upregulation of lncRNA HITT promotes cell apoptosis by suppressing the maturation of miR-602 in gastric cancer.

Histol Histopathol 2022 Jul 19:18495. Epub 2022 Jul 19.

Office of Academic Affairs of Jiangxi University of Traditional Chinese Medicine, Nanchang City, Jiangxi Province, PR China.

It has been reported that HITT can inhibit colon cancer. However, the role of HITT in gastric cancer (GC) is unknown. Our preliminary sequencing data revealed the altered expression of HITT in GC and its close correlation with miR-602, suggesting the involvement of HITT and its potential interaction with miR-602 in GC. This study explored the role of HITT and its crosstalk with miR-602 in GC. In this study, the expression of HITT, premature and mature miR-602 in paired GC and normal tissues (62 patients) was detected by RT-qPCR. RNA pull-down assay was performed to evaluate the direct interaction between HITT and mature miR-602. The subcellular location of HITT was assessed by nuclear fractionation assay. The role of HITT in regulating miR-602 maturation was explored by overexpression assay. Cell apoptosis was analyzed by flow cytometry. Our data illustrated that HITT was highly upregulated and mature miR-602 was downregulated in GC. No alteration in premature miR-602 in GC was observed. HITT was located in both nucleus and cytoplasm, and it can directly interact with miR-602. In addition, overexpression of HITT in GC cells increased the expression levels of mature miR-602 but not premature miR-602. Overexpression of HITT further increased GC cell apoptosis and suppressed the role of miR-602 in inhibiting GC cell apoptosis. In conclusion, HITT may promote GC cell apoptosis by suppressing the maturation of miR-602.
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http://dx.doi.org/10.14670/HH-18-495DOI Listing
July 2022

Identification of nucleoside monophosphates and their epigenetic modifications using an engineered nanopore.

Nat Nanotechnol 2022 Jul 18. Epub 2022 Jul 18.

State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China.

RNA modifications play critical roles in the regulation of various biological processes and are associated with many human diseases. Direct identification of RNA modifications by sequencing remains challenging, however. Nanopore sequencing is promising, but the current strategy is complicated by sequence decoding. Sequential nanopore identification of enzymatically cleaved nucleoside monophosphates may simultaneously provide accurate sequence and modification information. Here we show a phenylboronic acid-modified hetero-octameric Mycobacterium smegmatis porin A nanopore, with which direct distinguishing between monophosphates of canonical nucleosides, 5-methylcytidine, N-methyladenosine, N-methylguanosine, N-methyladenosine, inosine, pseudouridine and dihydrouridine was achieved. A custom machine learning algorithm, which reports an accuracy of 0.996, was also applied to the quantitative analysis of modifications in microRNA and natural transfer RNA. It is generally suitable for sensing of a variety of other nucleoside or nucleotide derivatives and may bring new insights to epigenetic RNA sequencing.
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http://dx.doi.org/10.1038/s41565-022-01169-2DOI Listing
July 2022

Cytochrome B5 type A alleviates HCC metastasis via regulating STOML2 related autophagy and promoting sensitivity to ruxolitinib.

Cell Death Dis 2022 Jul 18;13(7):623. Epub 2022 Jul 18.

Department of Hepatic Surgery, Key Laboratory of Hepatosplenic Surgery, Ministry of Education, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

The incidence of hepatocellular carcinoma (HCC) is increasing in the world. However, its role and underlying molecular mechanism in HCC progression remain unclear. We found that CYB5A plays a key role in HCC metastasis by inhibiting the JAK1/STAT3 pathway through binding to STOML2. CYB5A combined with STOML2 can predict the outcome of patients. To demonstrate the effect of CYB5A on JAK1 inhibitor function, we applied Ruxolitinib in metastatic tumors with high CYB5A expression and found that it slowed disease progression and prolonged survival in mice. To the best of our knowledge, this study is the first to report the Ruxolitinib effect on the metastatic ability of HCC cells in vivo and in vitro.
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http://dx.doi.org/10.1038/s41419-022-05053-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293983PMC
July 2022

Mechanisms of high-level fosfomycin resistance in Staphylococcus aureus epidemic lineage ST5.

J Antimicrob Chemother 2022 Jul 15. Epub 2022 Jul 15.

Department of Laboratory Medicine, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China.

Objectives: Fosfomycin resistance has become a clinical concern. In this study, we analysed the dynamic change of fosfomycin MIC in the epidemic Staphylococcus aureus lineages in a teaching hospital in Shanghai for 12 years and sought to elucidate the major underlying mechanisms.

Methods: MLST was conducted for 4580 S. aureus isolates recovered from 2008 to 2019. Fosfomycin MIC was determined by the agar dilution method. The genome data of 230 S. aureus epidemic lineage isolates were acquired from a next-generation sequencing (NGS) platform. Gene deletion and corresponding complementation mutants were constructed to confirm the mechanism of fosfomycin resistance.

Results: The predominant S. aureus lineages during the past 12 years were ST5 and ST239 (45.6%; 2090/4580). However, ST5 has been spreading clinically, while ST239 has gradually disappeared recently. Consistent with epidemic trends, fosfomycin-resistant ST5 increased from 19.5% to 67.3%. Most fosfomycin-resistant ST5 isolates (92.7%; 647/698) possessed high-level resistance (MIC > 1024 mg/L) with combined mutations mainly in glpT and uhpT. In contrast, fosfomycin-resistant ST239 isolates (76.8%; 149/194) mainly acquired low-level resistance (MIC = 64-128 mg/L) with mutation primarily in hptA. Deletion of a single resistant gene merely resulted in low-level fosfomycin resistance, while double-gene mutants ΔglpTΔuhpT, ΔglpTΔhptA and ΔglpTΔhptR acquired high-level fosfomycin resistance.

Conclusions: The high-level fosfomycin resistance of S. aureus epidemic lineage ST5 is mainly due to the accumulation of mutations in the resistant genes related to membrane transporter systems, and partly contributes to its persistent prevalence under clinical antibiotic pressure.
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http://dx.doi.org/10.1093/jac/dkac236DOI Listing
July 2022

Research on the Bioactivity of Plant Essential Oils on Armyworm [ (Walker)] Larvae.

Front Chem 2022 29;10:936873. Epub 2022 Jun 29.

Guizhou Light Industry Technical College, Guiyang, China.

In order to find out the biological activity of plant essential oils on armyworm [ (Walker, 1865)] larvae and provide a theoretical basis for the biological control of armyworms, in this study, the antifeedant activity, repellent activity, fumigation activity, contact activity, and synergistic effect on indoxacarb of nine kinds of plant essential oils on armyworm larvae were determined. The results showed that lavender and citronella essential oils had the greatest impact on the antifeedant activity on armyworm larvae, and the antifeedant rate reached 100.00%. Meanwhile, rosemary essential oil revealed the best repellent activity on armyworm larvae with an average dwell time of 0 s at the content of 0.2%. Moreover, tea tree essential oil and lemon essential oil at the content of 2.0% had the best fumigation and contact activity against armyworm larvae, and the corrected mortality rates at 120 h were 86.67 and 66.67%, respectively. In addition, the combination of citronella essential oil and indoxacarb with the ratio of 5:1 had the best synergistic effect on armyworm larvae at 96 h, and the synergistic ratio was reached 100.00%. These findings will guide the development of new insecticides for controlling armyworm larvae.
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http://dx.doi.org/10.3389/fchem.2022.936873DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277094PMC
June 2022

Tandem mass tagging combined with liquid chromatography-tandem mass spectrometry technique to detect protein markers in gastroesophageal junction adenocarcinoma.

Rapid Commun Mass Spectrom 2022 Sep;36(18):e9355

Department of Gastroenterology, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Background: Gastroesophageal junction adenocarcinoma (GEJA) is a malignant tumor located at the junction of the esophagus and stomach, the incidence of which is increasing year by year, while screening for early biomarkers is limited. Tandem mass tagging (TMT) coupled with liquid chromatography-tandem mass spectrometry (LC/MS/MS) has been used to screen for differential proteins in various cancers.

Methods: Differential proteins in GEJA and precancerous lesions were screened using TMT-LC/MS/MS, and then proteins that met expectations were selected for trend clustering analysis, combined with GO and KEGG analysis for functional annotation of differential proteins in GEJA. Then, parallel reaction monitoring and immunohistochemistry techniques were used to validate the accuracy of the proteomics data.

Results: Our group screened the differential proteins during GEJA progression using proteomics technology, analyzed the expression trends and functional regions involved in the differential proteins during carcinogenesis, and validated the accuracy of the experimental results.

Conclusions: The screening of differential proteins in GEJA carcinogenesis based on TMT-LC/MS/MS technology provides detailed information for the elucidation of GEJA progression process, pathogenesis, early screening and screening of candidate markers.
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http://dx.doi.org/10.1002/rcm.9355DOI Listing
September 2022

Open Reduction of Displaced Radial Neck Fractures in Children by Internal Fixation Techniques: Comparison of Percutaneous Kirschner Wiring and Elastic Stable Intramedullary Nailing.

Indian J Orthop 2022 Jul 2;56(7):1192-1198. Epub 2022 Apr 2.

Department of Orthopaedics, Children's Hospital of Soochow University, Suzhou, 215000 Jiangsu Province China.

Background: Although most paediatric radial neck fractures can be treated with closed reduction, some severely displaced fractures require open reduction. The purpose of this study is to compare the effects of ESIN and KW fixation in open reduction of radial neck fracture in children.

Methods: Twenty-four patients with mean age of 8.5 years were included. Four of the patients had a Judet type III fracture and 20 had a Judet type IV fracture. Ten patients who underwent percutaneous KW fixation were assigned to group A, while 14 patients who underwent ESIN fixation were assigned to group B. Variables of interest included age, sex, fracture type, associated lesions, surgical time, fracture reduction, cost, follow-up, healing time, X-rays, clinical outcomes, and complications.

Results: There were no significant between-group differences in sex, age, additional injuries, fracture type, and quality of reduction. Costs were significantly lower in Group A. Fracture healing was achieved in 23 of 24 patients (10/10 in group A and 13/14 in group B). In a postoperative elbow function assessment based on the Steele and Graham classification, 80% of patients in group A had a score of excellent or good, compared to 78.6% of patients in group B. Two cases of nail shifting and joint protrusion were observed in group B, one of which also presented with nonunion during follow-up.

Conclusions: Both KW and ESIN may achieve good clinical outcomes, but KW is associated with lower costs, easier implant removal (without the need for a secondary surgery), and lower iatrogenic complications.
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http://dx.doi.org/10.1007/s43465-022-00631-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232670PMC
July 2022

The Properties of Micro Carbon Fiber Composite Modified High-Viscosity Asphalts and Mixtures.

Polymers (Basel) 2022 Jul 2;14(13). Epub 2022 Jul 2.

School of Transportation Engineering, Shandong Jianzhu University, Jinan 250101, China.

In order to produce a high-viscosity asphalt and mixtures that can be used for ultra-thin overlays, high contents of Styrene-butadiene-styrene (SBS, 5%, 6%, 7%), styrene butadiene rubber (SBR, 1%, 2%, 3%) and micro carbon fiber (MCF, 0.8%) were used to modify conventional asphalt to prepare high-viscosity modified asphalt suitable for this purpose. The performance of the modified asphalts was evaluated by conventional index, kinematic viscosity, dynamic shear rheological test (DSR), multiple stress creep recovery test (MSCR), and bending beam rheometer test (BBR). The road performance of the modified asphalt mixtures was evaluated by high-temperature rutting, low-temperature bending, freeze-thaw splitting, fatigue, speckle, anti-skid, and water seepage tests. The results show that increasing the content of SBS can improve the high-temperature deformation resistance, low-temperature failure strain, kinematic viscosity, and viscosity toughness of modified asphalt, and the optimum content of SBS was 6%. SBR can improve the high-temperature performance, kinematic viscosity, and water damage resistance of modified asphalt, and the optimum dosage was 2%. Compared with 5% SBS-modified asphalt mixture, the dynamic stability, low-temperature failure strain, and freeze-thaw splitting strength ratio of 6% SBS + 0.8% MCF composite-modified asphalt mixture were increased by 48.7%, 24.7%, and 5.2% respectively. Compared with the 5% SBS-modified asphalt, the same characteristics of the 2% SBR + 5% SBS + 0.8% MCF composite-modified asphalt increased by 127.1%, 13.5%, and 5.5%, respectively. Compared with 5% SBS-modified asphalt, the fatigue performance of 6% SBS + 0.8% MCF-modified asphalt was improved by 32.2%. The kinematic viscosity of 6% SBS + 0.8% MCF and 5% SBS + 0.8% MCF + 2% SBR modified asphalt met the performance requirements of high-viscosity asphalt and had excellent road performance. It can be applied to ultra-thin overlays to optimize its adhesion with the original pavement.
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http://dx.doi.org/10.3390/polym14132718DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269128PMC
July 2022

Characteristics of the Ciliary Body in Healthy Chinese Subjects Evaluated by Radial and Transverse Imaging of Ultrasound Biometric Microscopy.

J Clin Med 2022 Jun 27;11(13). Epub 2022 Jun 27.

State Key Laboratory of Ophthalmology, Guangzhou 510060, China.

Background: The imaging and analysis of the ciliary body (CB) are valuable in many potential clinical applications. This study aims to demonstrate the anatomy characteristics of CB using radial and transverse imaging of ultrasound biometric microscopy (UBM) in healthy Chinese subjects, and to explore the determining factors.

Methods: Fifty-four eyes of 30 healthy Chinese subjects were evaluated. Clinical data, including age, body mass index (BMI), intraocular pressure (IOP), axial length (AL), and lens thickness (LT), were collected. Radial and transverse UBM measurements of the ciliary body were performed. Anterior chamber depth (ACD), ciliary sulcus diameter (CSD), ciliary process length (CPL), ciliary process density (CPD), ciliary process area (CPA), ciliary muscle area (CMA), ciliary body area (CBA), ciliary body thickness (CBT, CBT, and CBT), anterior placement of ciliary body (APCB), and trabecular-ciliary angle (TCA) of four (superior, nasal, inferior, and temporal) quadrants were measured.

Results: The average CPL was 0.513 ± 0.074 mm, and the average CPA was 0.890 ± 0.141 mm. CPL and CPA tended to be longer and larger in the superior quadrant ( < 0.001) than in the other three quadrants. Average CPL was significantly correlated with AL (r = 0.535, < 0.001), ACD (r = 0.511, < 0.001), and LT (r = -0.512, < 0.001). Intraclass correlation coefficient (ICC) scores were high for CPL (0.979), CPD (0.992), CPA (0.966), CMA (0.963), and CBA (0.951).

Conclusions: In healthy Chinese subjects, CPL was greatest in the superior quadrant, followed by the inferior, temporal, and nasal quadrants, and CPA was largest in the superior quadrant, followed by the tempdoral, inferior, and nasal quadrants. Transverse UBM images can be used to measure the anatomy of the ciliary process with relatively good repeatability and reliability.
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http://dx.doi.org/10.3390/jcm11133696DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267437PMC
June 2022

Broadening prime editing toolkits using RNA-Pol-II-driven engineered pegRNA.

Mol Ther 2022 Jul 6. Epub 2022 Jul 6.

School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China. Electronic address:

The prime editor is a versatile tool for targeted precise editing to generate point mutations, small insertions, or small deletions in eukaryotes. However, canonical PE3 system is less efficient, notably in primary cells or pluripotent stem cells. Here, we employed RNA polymerase II promoter instead of RNA polymerase III promoter, whose application is limited by specific DNA contexts, to produce Csy4-processed intronic prime editing guide RNAs (pegRNAs) and, together with other optimizations, achieved efficient targeting with poly(T)-containing pegRNAs, as well as combinatorial and conditional genetic editing. We also found simultaneous suppression of both DNA mismatch repair and DNA damage response could achieve efficient and accurate editing in human embryonic stem cells. These findings relieve the restrictions of RNA polymerase III (RNA-Pol-III)-based base editors and broadened the applications of prime editing.
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http://dx.doi.org/10.1016/j.ymthe.2022.07.002DOI Listing
July 2022

A polyaniline surface-modified Prussian blue analogue cathode for flexible aqueous Zn-ion batteries.

Chem Commun (Camb) 2022 Jul 21;58(59):8226-8229. Epub 2022 Jul 21.

Sauvage Laboratory for Smart Materials, School of Materials Science and Engineering, Harbin Institute of Technology, Shenzhen, 518055, China.

Aqueous Zn-ion batteries have gained popularity due to their low cost and high safety, but their low energy density limits their application scenarios. Although the Prussian blue analogue (PBA) has the characteristics of high redox potential, the poor capacity and cycling performance restrict its further development. Here, we propose a modification strategy of a polyaniline (PANI) coating on zinc ferricyanide (ZnHCF). The PANI coating inhibits the dissolution of ZnHCF and enables the Zn-ion battery to present two long-flat discharge voltage platforms as well as a high capacity of 150 mA h g, which provides a new idea for the development of high-performance PBA battery materials. Meanwhile, owing to the spring-like structure, the battery has a high stretchability of 600% and maintains stable electrochemical properties during stretching.
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http://dx.doi.org/10.1039/d2cc02724kDOI Listing
July 2022

Detection of Pyrophosphate and Alkaline Phosphatase Activity Based on PolyT Single Stranded DNA - Copper Nanoclusters.

J Fluoresc 2022 Jul 1. Epub 2022 Jul 1.

Hunan Province Key Laboratory for Forestry Biotechnology, College of Life Science and technology, Central South University of Forestry and Technology, 410004, Changsha, Hunan Province, China.

The determination of pyrophosphate and alkaline phosphatase activity plays a significant role in medical diagnosis. In this work, a label-free "ON-OFF-ON" fluorescence strategy is developed for the analysis of pyrophosphate and alkaline phosphatase activity. Using PolyT single strand DNA as templates to synthesize fluorescent copper nanoparticles, the coordination effect of pyrophosphoric acid on Cu inhibited the generation of fluorescence. Afterwards, the addition of alkaline phosphatase into hydrolyze pyrophosphoric acid resulted in the release of Cu whereby the fluorescence intensity could be recovered. Thereupon enhanced-sensitivity for alkaline phosphatase was obtained (0.1 mU/L), much better than previously reported methods. Meanwhile, it could be performed directly in homogeneous solution, which was very close to the actual activity level of alkaline phosphatase under physiological conditions. Likewise, satisfactory results were also obtained in specificity assessment, which demonstrated its potential application in clinical diagnosis. Notably, a new, sensitive, low-cost, short-time, and high-sensitivity platform for alkaline phosphatase detection was constructed, and the design of biosensor using DNA-templated Copper nanoclusters (CuNCs) was instructed in this study.
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http://dx.doi.org/10.1007/s10895-022-02984-5DOI Listing
July 2022

Deep coverage proteome analysis of hair shaft for forensic individual identification.

Forensic Sci Int Genet 2022 Jun 18;60:102742. Epub 2022 Jun 18.

Graduate School, People's Public Security University of China, No.1 Muxidi Nanli, Beijing 100038, China; National Engineering Laboratory for Forensic Science, Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, No.17 Muxidi Nanli, Beijing 100038, China. Electronic address:

Hair shaft is one of the most common biological evidence found at crime scenes. However, due to the biogenic degradation of nuclear DNA in hair shaft, it is difficult to achieve individual identification through routine DNA analysis. In contrast, the proteins in hair shaft are stable and contain genetic polymorphisms in the form of single amino acid polymorphisms (SAPs), translated from non-synonymous single nucleotide polymorphisms (nsSNPs) in the genome. However, the number of SAPs detected still cannot meet the requirements of practical applications. This paper developed a deep coverage proteome analysis method by combining a three-step sequential ionic liquid-based protein extraction and 2D-RPLC-MS/MS with high and low pH to identify both variant and reference SAPs from 2-cm-long hair shafts. We identified 632 ± 243 protein groups from 10 individuals, with the average number of SAPs reaching 167 ± 21/person. These were further used to calculate random match probabilities (RMPs), a widely accepted forensic statistical term for human identification. The RMPs ranged from 6.53 × 10 to 3.10 × 10 (median = 2.62 × 10) when calculated with frequency of matching nsSNP genotype data from exomes, and ranged from 2.62 × 10 to 2.07 × 10 (median = 4.88 × 10) with SAP genotype frequency. All these results indicate that the deep coverage proteomics method is beneficial for improving SAP-based forensic individual identification in hair shaft, with great potential in crime investigation.
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http://dx.doi.org/10.1016/j.fsigen.2022.102742DOI Listing
June 2022
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