Publications by authors named "Yao Li"

1,967 Publications

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Efficacy of glycine powder air-polishing in supportive periodontal therapy: a systematic review and meta-analysis.

J Periodontal Implant Sci 2021 Jun;51(3):147-162

College of Stomatology, Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China.

Purpose: This systematic review and meta-analysis was conducted to assess the effects of glycine powder air-polishing (GPAP) in patients during supportive periodontal therapy (SPT) compared to hand instrumentation and ultrasonic scaling.

Methods: The authors searched for randomized clinical trials in 8 electronic databases for relevant studies through November 15, 2019. The eligibility criteria were as follows: population, patients with chronic periodontitis undergoing SPT; intervention and comparison, patients treated by GPAP with a standard/nozzle type jet or mechanical instrumentation; and outcomes, bleeding on probing (BOP), patient discomfort/pain (assessed by a visual analogue scale [VAS]), probing depth (PD), gingival recession (Rec), plaque index (PI), clinical attachment level (CAL), gingival epithelium score, and subgingival bacteria count. After extracting the data and assessing the risk of bias, the authors performed the meta-analysis.

Results: In total, 17 studies were included in this study. The difference of means for BOP in patients who received GPAP was lower (difference of means: -8.02%; 95% confidence interval [CI], -12.10% to -3.95%; <0.00001; I²=10%) than that in patients treated with hand instrumentation. The results of patient discomfort/pain measured by a VAS (difference of means: -1.48, 95% CI, -1.90 to -1.06; <0.001; I²=83%) indicated that treatment with GPAP might be less painful than ultrasonic scaling. The results of PD, Rec, PI, and CAL showed that GPAP had no advantage over hand instrumentation or ultrasonic scaling.

Conclusions: The findings of this study suggest that GPAP may alleviate gingival inflammation more effectively and be less painful than traditional methods, which makes it a promising alternative for dental clinical use. With regards to PD, Rec, PI, and CAL, there was insufficient evidence to support a difference among GPAP, hand instrumentation, and ultrasonic scaling. Higher-quality studies are still needed to assess the effects of GPAP.
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http://dx.doi.org/10.5051/jpis.1902340117DOI Listing
June 2021

PGC-1 Protects against Hepatic Ischemia Reperfusion Injury by Activating PPAR and PPAR and Regulating ROS Production.

Oxid Med Cell Longev 2021 19;2021:6677955. Epub 2021 May 19.

Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.

Peroxisome proliferator-activated receptors (PPARs) and have been shown to be protective in hepatic ischemia/reperfusion (I/R) injury. However, the precise role of PPAR coactivator-1 (PGC-1), which can coactivate both of these receptors, in hepatic I/R injury, remains largely unknown. This study was designed to test our hypothesis that PGC-1 is protective during hepatic I/R injury in vitro and in vivo. Our results show that endogenous PGC-1 is basally expressed in normal livers and is moderately increased by I/R. Ectopic PGC-1 protects against hepatic I/R and hepatocyte anoxia/reoxygenation (A/R) injuries, whereas knockdown of endogenous PGC-1 aggravates such injuries, as evidenced by assessment of the levels of serum aminotransferases and inflammatory cytokines, necrosis, apoptosis, cell viability, and histological examination. The EMSA assay shows that the activation of PPAR and PPAR is increased or decreased by the overexpression or knockdown of PGC-1, respectively, during hepatic I/R and hepatocyte A/R injuries. In addition, the administration of specific antagonists of either PPAR (MK886) or PPAR (GW9662) can effectively decrease the protective effect of PGC-1 against hepatic I/R and hepatocyte A/R injuries. We also demonstrate an important regulatory role of PGC-1 in reactive oxygen species (ROS) metabolism during hepatic I/R, which is correlated with the induction of ROS-detoxifying enzymes and is also dependent on the activations of PPAR and PPAR. These data demonstrate that PGC-1 protects against hepatic I/R injury, mainly by regulating the activation of PPAR and PPAR. Thus, PGC-1 may be a promising therapeutic target for the protection of the liver against I/R injury.
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http://dx.doi.org/10.1155/2021/6677955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159639PMC
May 2021

Multiple investigations of aqueous Eu(III)-oxalate complexes: the reduction in coordination number and validation of spectral linear correlation.

Dalton Trans 2021 Jun 7. Epub 2021 Jun 7.

Beijing National Laboratory for Molecular Sciences, Fundamental Science Laboratory on Radiochemistry and Radiation Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.

Detailed information on the An(iii)/Ln(iii) complexation properties in solution is essential for separation chemistry and the prediction of their potential for radionuclide migration from nuclear waste repositories into natural aquifers. In the present study, to better reveal and confirm the structural information of [Eu(Ox)x (H2O)h-2x]3-2x (h = 8, 9; x = 0-3) aqueous species, especially the variable coordination number (CN), and explore the validity of the spectral linear correlation between the luminescence lifetime and the residual hydration number in the first coordination sphere of Eu(iii) compounds in solution, a comparison between the spectral results and the theoretical calculations in a wide parametric space in terms of the pH value and oxalate concentration was carried out by combining time-resolved luminescence spectroscopy (TRLS) with speciation modelling and density functional theory (DFT) calculations. We have found direct and clear evidence for the 9-fold to 8-fold coordination number reduction of Eu(iii) atoms upon coordination with more than one oxalate in an aqueous medium, and as well systematically validated the applicability of the spectral linear correlation in an aqueous system (otherwise solid state) involving multiple species with the support of relatively reliable and clear speciation modelling.
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http://dx.doi.org/10.1039/d1dt00609fDOI Listing
June 2021

Challenges in establishing a strong family medicine system in China.

Fam Pract 2021 Jun 7. Epub 2021 Jun 7.

Psychosomatic Medical Center, The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Laboratory for Neuroinformation, University of Electronic Science and Technology of China, Chengdu, China.

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http://dx.doi.org/10.1093/fampra/cmab059DOI Listing
June 2021

Development and validation of sensitive methods for simultaneous determination of 9 antiviral drugs in different various environmental matrices by UPLC-MS/MS.

Chemosphere 2021 May 28;282:131047. Epub 2021 May 28.

SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Environmental Theoretical Chemistry, School of Environment, South China Normal University, Guangzhou, 510006, China. Electronic address:

Trace antiviral drug contamination in aquatic ecosystems is becoming a significant environmental concern that requires an urgent efficient determination method. Here we developed sensitive and robust multi-residue determination methods to simultaneously extract and analyze 9 commonly used antiviral drugs (abacavir, zidovudine, efavirenz, nevirapine, ritonavir, lopinavir, lamivudine, telbivudine and entecavir) in surface water, wastewater, sediment, and sludge. Water samples were extracted with solid-phase extraction (SPE) technique using tandem hydrophilic-lipophilic balance and graphitized carbon black cartridges, while sediment and sludge samples were extracted using QuEChERS (quick, easy, cheap, effective, rugged, and safe) method. The extraction conditions of SPE (pH and cartridge type) and QuEChERS (acetic acid content, salts reagent, and purification sorbent) methods were carefully optimized. We observed that under optimum conditions, the method quantification limits of the 9 antiviral drugs in water and solid samples ranged from 0.05 to 19.23 ng L and from 0.02 to 7.38 ng g, respectively. For environmental samples spiking 3 different concentrations, the recovery values for the most targeted antiviral drugs ranged from 70 to 130%, except for efavirenz. All targeted antiviral drugs were detected in wastewater samples except for entecavir. We also found abacavir, efavirenz, ritonavir, lopinavir, and telbivudine in sediment and sludge samples. Notably, telbivudine was identified in all environmental matrices, with a high concentration of 127 ng L and 222 ng g in water and sediment samples, respectively.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131047DOI Listing
May 2021

Prohexadione-calcium alleviates saline-alkali stress in soybean seedlings by improving the photosynthesis and up-regulating antioxidant defense.

Ecotoxicol Environ Saf 2021 Jun 2;220:112369. Epub 2021 Jun 2.

College of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China; Agricultural College of Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163000, China; Shenzhen Research Institute of Guangdong Ocean University, Shenzhen, Guangdong 518108, China. Electronic address:

Soil salinization seriously restricts the growth and yield of soybeans. However, little information is available on the early growth stages of soybeans which are subjected to the gibberellin biosynthesis inhibitor, prohexadione-calcium (Pro-Ca). This study aimed to investigate the effects of exogenous Pro-Ca on saline-alkali stress-induced damages to photosynthesis and antioxidant defenses in soybean (Glycine max L.) seedlings. At the V3 growth stage, salt-tolerant genotype Hefeng 50 (HF50) and salt-sensitive genotype Kenfeng 16 (KF16) were subjected to 110 mmol L mixed saline-alkali stress respectively, and then 100 mg L Pro-Ca was sprayed on the leaves. Our results showed that saline-alkali stress accelerated the degradation of thylakoids, inhibited chlorophyll synthesis, reduced shoot dry weight, electron transfer rate (ETR), and peroxidase (POD) activity, the concentration of ascorbic acid (AsA) and soluble sugar, but enhanced the concentration of proline, hydrogen peroxide (HO) and the rate of superoxide radical (O) generation. Additionally, saline-alkali stress induced a lower decrease of the net photosynthetic rate (P), potential activity of PSII (F/F), and maximum quantum yield of PSII (F/F) in salt-tolerant HF50 than in salt-sensitive KF16. Nevertheless, foliar spraying of exogenous Pro-Ca increased the chlorophyll content, P, F/F, and F/F. These results were more prominent when Pro-Ca was applied to KF16 under saline-alkali conditions. Furthermore, exogenous application of Pro-Ca retarded the degradation of thylakoids, increased the ETR and the accumulation of AsA, soluble sugar, and proline, activated the activities of superoxide dismutase (SOD), catalase (CAT), and POD, and decreased the concentration of malondialdehyde (MDA), electrolyte leakage (EL), O, and HO. These results indicated that Pro-Ca could effectively protect soybean seedlings against damage from saline-alkali stress by regulating seedling phenotype, photosynthetic apparatus, antioxidant defense, and osmoregulation.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112369DOI Listing
June 2021

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) moonlights as an adhesin in Mycoplasma hyorhinis adhesion to epithelial cells as well as a plasminogen receptor mediating extracellular matrix degradation.

Vet Res 2021 Jun 3;52(1):80. Epub 2021 Jun 3.

Institute of Veterinary Medicine, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

Mycoplasma hyorhinis infects pigs causing polyserositis and polyarthritis, and has also been reported in a variety of human tumor tissues. The occurrence of disease is often linked with the systemic invasion of the pathogen. Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), one of the key enzymes of glycolysis, was reported as a surface multifunctional molecule in several bacteria. Here, we investigated whether GAPDH could manifest binary functions; as an adhesin to promote colonization as well as a plasminogen receptor functioning in extracellular matrix (ECM) degradation to promote systemic invasion. The surface localization of GAPDH was observed in M. hyorhinis with flow cytometry and colony blot analysis. Recombinant GAPDH (rGAPDH) was found to be able to bind porcine-derived PK-15 and human-derived NCI-H292 cells. The incubation with anti-GAPDH antibody significantly decreased the adherence of M. hyorhinis to both cell lines. To investigate its function in recruiting plasminogen, firstly, the interaction between rGAPDH and plasminogen was demonstrated by ELISA and Far-Western blot assay. The activation of the rGAPDH-bound plasminogen into plasmin was proved by using a chromogenic substrate, and furtherly confirmed to degrade extracellular matrix by using a reconstituted ECM. Finally, the ability of rGAPDH to bind different ECM components was demonstrated, including fibronectin, laminin, collagen type IV and vitronectin. Collectively, our data imply GAPDH as an important adhesion factor of M. hyrohinis and a receptor for hijacking host plasminogen to degrade ECM. The multifunction of GAPDH to bind both plasminogen and ECM components is believed to increase the targeting of proteolysis and facilitate the dissemination of M. hyorhinis.
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http://dx.doi.org/10.1186/s13567-021-00952-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173509PMC
June 2021

Quality Changes of Clinical Practice Guidelines for Respiratory Diseases in China: A Systematic Review.

Clin Respir J 2021 Jun 2. Epub 2021 Jun 2.

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, China.

After the low quality of Chinese clinical practice guidelines (CPGs) for respiratory diseases published from 1979 to 2013 were reported, some handbooks were published to standardize guidelines' development recently. There was a great increase in production and dissemination of CPGs annually in China, whose quality and potential impact were unknown. A systematic search of four literature databases was performed for the period January 2013 to December 2018 to identify Chinese CPGs for respiratory diseases. Eligible CPGs were evaluated using the Appraisal of Guidelines for Research and Evaluation II (AGREE II) instrument. A total of 197 CPGs were identified for review. Compared with the result of previous study, the increased scores of the six AGREE II domains were screened: Scope and purpose (57.3% vs. 57.8%), Stakeholder involvement (17.6% vs. 25.0%), Rigor of development (10.2% vs. 13.2%), Clarity and presentation (55.2% vs. 58.4%), Applicability (9.3% vs. 25.9%), Editorial independence (1.1% vs. 6.3%). The improved overall assessment for included CPGs were: Recommended (4, 2.0% vs. 0, 0%), Recommended with modifications (26, 13.2% vs. 3, 2.8%). The improved level of evidence used to make recommendations were: (59, 11.9% vs. 168, 22.4%; 88, 17.7% vs. 195, 26.0%, A, B, respectively) The overall quality of CPGs for respiratory diseases published from 2013 to 2018 in China was slightly improved, but had a big gap with the optimum level, especially in Rigor of development and Editorial independence. Increased efforts are required to enable the development of high-quality evidence-based CPGs for respiratory diseases.
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http://dx.doi.org/10.1111/crj.13402DOI Listing
June 2021

Distinct expression requirements and rescue strategies for loss- and gain-of-function mutations.

Elife 2021 Jun 1;10. Epub 2021 Jun 1.

Department of Pharmacology and Physiology, University of Rochester, School of Medicine and Dentistry, Rochester, United States.

Genetic mutation of the human gene, which encodes a Ca-activated Cl channel (BEST1) predominantly expressed in retinal pigment epithelium (RPE), causes a spectrum of retinal degenerative disorders commonly known as bestrophinopathies. Previously, we showed that BEST1 plays an indispensable role in generating Ca-dependent Cl currents in human RPE cells, and the deficiency of BEST1 function in patient-derived RPE is rescuable by gene augmentation (Li et al., 2017). Here, we report that patient-derived loss-of-function and gain-of-function mutations require different mutant to wild-type (WT) molecule ratios for phenotypic manifestation, underlying their distinct epigenetic requirements in bestrophinopathy development, and suggesting that some of the previously classified autosomal dominant mutations actually behave in a dominant-negative manner. Importantly, the strong dominant effect of gain-of-function mutations prohibits the restoration of BEST1-dependent Cl currents in RPE cells by gene augmentation, in contrast to the efficient rescue of loss-of-function mutations via the same approach. Moreover, we demonstrate that gain-of-function mutations are rescuable by a combination of gene augmentation with CRISPR/Cas9-mediated knockdown of endogenous expression, providing a universal treatment strategy for all bestrophinopathy patients regardless of their mutation types.
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http://dx.doi.org/10.7554/eLife.67622DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169119PMC
June 2021

Will Proton Pump Inhibitors Lead to a Higher Risk of COVID-19 Infection and Progression to Severe Disease? A Meta-analysis.

Jpn J Infect Dis 2021 May 31. Epub 2021 May 31.

Radiation Oncology, Mayo Clinic, USA.

Previous researches on the association between proton pump inhibitors (PPIs) use and the treatment and prevention of COVID-19 has generated inconsistent findings. Therefore, this meta-analysis was conducted to clarify the outcome in patients who take PPIs. Eight articles with more than 268,683 subjects were included. PPI use was not associated with increased or decreased risk of COVID-19 infection (OR:3.16, 95% CI=0.74-13.43, P=0.12) or mortality risk of COVID-19 patients (OR=1.91, 95% CI=0.86-4.24, P=0.11). While it can add risk of severe disease (OR=1.54, 95% CI=1.20-1.99, P<0.001;) and secondary infection (OR=4.33, 95% CI=2.57-7.29). In summary, PPI use is not associated with an increased risk of infection and may not change the mortality risk of COVID-19, but appeared to be associated with an increased risk of progression to severe disease and secondary infection. However, more original studies to further clarify the relationship between PPI and COVID-19 are still urgently needed.
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http://dx.doi.org/10.7883/yoken.JJID.2021.074DOI Listing
May 2021

Recent progress of personal glucose meters integrated methods in food safety hazards detection.

Crit Rev Food Sci Nutr 2021 May 28:1-14. Epub 2021 May 28.

Engineering Research Center of Bio-Process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei, China.

Development of personal glucose meters (PGMs) for blood glucose monitoring and management by the diabetic patients has been a long history since its first invention in 1968 and commercial application in 1975. The main reasons for its wide acceptance and popularity can be attributed mainly to the easy operation, test-to-result model, low cost, and small volume of sample required for blood glucose concentration test. During past decades, advances in analytical techniques have repurposed the use of PGMs into a general point-of-care testing platform for a variety of non-glucose targets, especially the food hazards. In this review, we summarized the recent published research using PGMs to detect the food safety hazards of mycotoxins, illegal additives, pathogen bacteria, and pesticide and veterinary drug residues detection with PGMs. The progress on PGM-based detection achieved in food safety have been carefully compared and analyzed. Furthermore, the current bottlenecks and challenges for practical applications of PGM for hazards detection in food safety have also been proposed.
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http://dx.doi.org/10.1080/10408398.2021.1913990DOI Listing
May 2021

Cellulose nanocrystals reinforced highly stretchable thermal-sensitive hydrogel with ultra-high drug loading.

Carbohydr Polym 2021 Aug 27;266:118122. Epub 2021 Apr 27.

Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China. Electronic address:

Hydrogels often have poor mechanical properties which limit their application in load-bearing tissues such as muscle and cartilage. In this work, a near-infrared light-triggered stretchable thermal-sensitive hydrogel with ultra-high drug loading was developed by a combination of natural polymeric nanocrystals, a network of synthetic thermo-responsive polymer, and magnetic FeO nanoparticles. The hydrogels comprise cellulose nanocrystals (CNCs) decorated with FeO nanoparticles (FeO/CNCs) dispersed homogeneously in poly(N-isopropylacrylamide) (PNIPAm) networks. The composite hydrogels exhibit an extensibility of 2200%. Drug loading of vancomycin (VCM) reached a high value of 10.18 g g due to the dispersion of FeO/CNCs and the interactions between the CNCs and the PNIPAm network. Importantly, the hydrogels demonstrated a thermo-response triggered by NIR, with the temperature increasing from 26 to 41 °C within 60 s. The hydrogels have high biocompatibility evidenced by cell proliferation tests, illustrating that these hydrogels are promising as dressings for wound closure, and wound healing.
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http://dx.doi.org/10.1016/j.carbpol.2021.118122DOI Listing
August 2021

Potential Predictors and Survival Analysis of the Relapse of HIV-Associated Cryptococcal Meningitis: A Retrospective Study.

Front Med (Lausanne) 2021 10;8:626266. Epub 2021 May 10.

Division of Infectious Diseases, Chongqing Public Health Medical Center, Chongqing, China.

We intend to investigate the relapse of HIV-associated cryptococcal meningitis (CM), assess potential predictors and conduct survival analysis, with a view to establishing a valid reference for the management of the relapse of CM. This is a retrospective study in Chinese patients with HIV-associated CM and those who experience relapse of CM. Baseline demographic, laboratory and clinical characteristics of patients with HIV-associated CM were collected. Predictors for relapse of HIV-associated CM were analyzed using univariate and multivariate logistic regression. Survival probability in relapse cases was determined by Kaplan-Meier survival curves. During the study period, 87 of 348 (25.0%) HIV patients experienced the relapse of CM. CD4+ T-cell counts, antiretroviral therapy (ART) status and the time from symptom onset to presentation were all statistically associated with the relapse of CM ( = 0.013, 0.018 and 0.042, respectively). The overall survival among 46 HIV CM relapse patients whose survival information were obtained, was 78.3%. The proportion of patients who died after antifungal treatment for CM was greater in those whose interval from symptom onset to presentation ≥4 weeks, compared with those <4 weeks ( = 0.0331). In order to reduce the relapse of CM and increase the survival possibility of these patients, we can promote the importance of ART before CM occurs, emphasize timely consultation when any CM-associated clinical symptoms occurs, and individualized the timing of ART initiation according to indicators which can reflect the severity of CM.
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http://dx.doi.org/10.3389/fmed.2021.626266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141581PMC
May 2021

Stationary distributions of persistent ecological systems.

J Math Biol 2021 May 26;82(7):64. Epub 2021 May 26.

Department of Mathematics and Statistics, University of Massachusetts Amherst, 710 N Pleasant Street, Amherst, MA, 01003, USA.

We analyze ecological systems that are influenced by random environmental fluctuations. We first provide general conditions which ensure that the species coexist and the system converges to a unique invariant probability measure (stationary distribution). Since it is usually impossible to characterize this invariant probability measure analytically, we develop a powerful method for numerically approximating invariant probability measures. This allows us to shed light upon how the various parameters of the ecosystem impact the stationary distribution. We analyze different types of environmental fluctuations. At first we study ecosystems modeled by stochastic differential equations. In the second setting we look at piecewise deterministic Markov processes. These are processes where one follows a system of differential equations for a random time, after which the environmental state changes, and one follows a different set of differential equations-this procedure then gets repeated indefinitely. Finally, we look at stochastic differential equations with switching, which take into account both the white noise fluctuations and the random environmental switches. As applications of our theoretical and numerical analysis, we look at competitive Lotka-Volterra, Beddington-DeAngelis predator-prey, and rock-paper-scissors dynamics. We highlight new biological insights by analyzing the stationary distributions of the ecosystems and by seeing how various types of environmental fluctuations influence the long term fate of populations.
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http://dx.doi.org/10.1007/s00285-021-01613-2DOI Listing
May 2021

Salient Constructs for the Development of Shared Decision-Making Tools for HIV Pre-Exposure Prophylaxis Uptake and Regimen Choice: Behaviors, Behavioral Skills, and Beliefs.

AIDS Patient Care STDS 2021 Jun 25;35(6):195-203. Epub 2021 May 25.

Division of Infectious Disease, Chongqing Public Health Medical Center, Chongqing, China.

The availability of multiple pre-exposure prophylaxis (PrEP) regimens creates a preference-sensitive choice for individuals weighing alternative HIV prevention strategies. Investigating factors that are associated with PrEP uptake and regimen choice are key to developing tools to support that decision. A cross-sectional survey was offered to 536 participants in a PrEP demonstration project for gay, bisexual, and other men who have sex with men across 3 cities in China, of which 412 completed surveys and were included in the analysis. We conducted bivariable analyses followed by multi-variable logistic regressions to examine factors associated with overall PrEP uptake and PrEP regimen choice. Among the 412 participants, 36.9% were daily PrEP users, 28.4% were event-driven users, and 34.7% were non-PrEP users. Higher levels of education, lower PrEP use stigma, and higher perceived benefits of PrEP were associated with greater PrEP uptake. More frequent sex, higher PrEP adherence self-efficacy, higher endorsement of the belief in elevated efficacy of daily over on-demand PrEP, and lower endorsement of a desire for a PrEP method that is only taken when needed led to greater odds of choosing the daily PrEP regimen. As multiple HIV prevention modalities become available, there is a greater need for a shared decision-making approach to support this preference-sensitive choice of HIV prevention method. Our findings suggest salient constructs to inform the development of shared decision-making tools to support regimen choice.
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http://dx.doi.org/10.1089/apc.2021.0066DOI Listing
June 2021

Catalytic Asymmetric Aza-Diels-Alder Reaction of Ketimines and Unactivated Dienes.

Angew Chem Int Ed Engl 2021 May 25. Epub 2021 May 25.

Nankai University College of Chemistry, Department of Chemistry, CHINA.

The enantioselective aza-Diels-Alder reaction represents one of the most efficient method for constructing chiral tetrahydropyridines. Nevertheless, the catalytic asymmetric aza-Diels-Alder reaction of ketimines with unactivated dienes is still a challenging topic. Herein, guided by computational screening, a highly enantioselective aza-Diels-Alder reaction of 2-aryl-3 H -indol-3-ones with unactivated dienes was realized by using a B(C 6 F 5 ) 3 /chiral phosphoric acid catalyst system under mild conditions. The reaction has a broad scope with respect to both aza-Diels-Alder reaction partners and hence offers rapid access to an array of tetrahydropyridine derivatives with pretty outcomes (up to 99% yield, >20:1 dr and 98:2 er). Notely, the reaction is very efficient, in which the catalyst loadings for the model reaction can be reduced to 0.1 mol% and the enantioselectivity is still maintained. Besides, the synthetic utility of the protocol was proved by transformations of the products. Moreover, DFT calculations provide convincing evidence for the interpretation of stereoselection.
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http://dx.doi.org/10.1002/anie.202104788DOI Listing
May 2021

Stress controllability modulates basal activity of dopamine neurons in the substantia nigra compacta.

eNeuro 2021 May 21. Epub 2021 May 21.

MOE Key Laboratory of Modern Teaching Technology, Shaanxi Normal University, Xi'an 710062, China

Prolonged stress induces neural maladaptations in mesolimbic dopamine (DA) system and produces emotional and behavioral disorders. However, the effects of stress on activity of DA neurons are diverse and complex that hinge on the type, duration, intensity, and controllability of stressors. Here, controlling the duration, intensity, and type of the stressors to be identical, we observed effects of stressor controllability on the activity of substantia nigra compacta (SNc) DA neurons in mice. We found that both lack and loss of control over shock enhance the basal activity and intrinsic excitability of SNc DA neurons via modulation of I current, but not via corticosterone serum level. Moreover, loss of control over shock produces more significant enhancement in the basal activity of SNc DA neurons than that produced shock per se, and therefore attenuates the response to natural reward. This attenuation can be reversed by control over shock. These results indicate that although chronic stress per se tends to enhance the basal activity of SNc DA neurons, loss of control over the stressor is able to induce a larger enhancement in basal activity of SNc DA neurons and produce more severe behavioral deficits. However, control over stress ameliorates the deleterious effects of stress, highlighting the role of stress controllability.The impact of stress on the DA system significantly modifies immediate and guide future behaviors. Stress does not have unitary effects on VTA DA neurons, but the effects of stress controllability on SNc DA neurons are unclear. The present work studied the effects of controllability on the activity of SNc DA neurons by controlling the duration, intensity, and pattern of footshocks to be identical. The results show that loss of control over shock produces larger enhancement in basal activity of SNc DA neurons and worse behavioral deficits than what caused by uncontrollable shock per se. The results demonstrate the critical role of stress controllability in modulating activity of SNc DA neurons and inducing behavioral deficits.
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http://dx.doi.org/10.1523/ENEURO.0044-21.2021DOI Listing
May 2021

Electronic Modulation of Non-van der Waals 2D Electrocatalysts for Efficient Energy Conversion.

Adv Mater 2021 May 25:e2008422. Epub 2021 May 25.

School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, 637459, Singapore.

The exploration of efficient electrocatalysts for energy conversion is important for green energy development. Owing to their high surface areas and unusual electronic structure, 2D electrocatalysts have attracted increasing interest. Among them, non-van der Waals (non-vdW) 2D materials with numerous chemical bonds in all three dimensions and novel chemical and electronic properties beyond those of vdW 2D materials have been studied increasingly over the past decades. Herein, the progress of non-vdW 2D electrocatalysts is critically reviewed, with a special emphasis on electronic structure modulation. Strategies for heteroatom doping, vacancy engineering, pore creation, alloying, and heterostructure engineering are analyzed for tuning electronic structures and achieving intrinsically enhanced electrocatalytic performances. Lastly, a roadmap for the future development of non-vdW 2D electrocatalysts is provided from material, mechanism, and performance viewpoints.
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http://dx.doi.org/10.1002/adma.202008422DOI Listing
May 2021

Middle temporal corpus callosum impairment as a predictor of eight-week treatment outcome of drug-naïve first-episode psychosis patients: A pilot longitudinal study.

Schizophr Res 2021 May 21;232:95-97. Epub 2021 May 21.

School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China. Electronic address:

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http://dx.doi.org/10.1016/j.schres.2021.05.005DOI Listing
May 2021

Comprehensive analysis of the stress associated protein (SAP) gene family in Tamarix hispida and the function of ThSAP6 in salt tolerance.

Plant Physiol Biochem 2021 May 14;165:1-9. Epub 2021 May 14.

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China. Electronic address:

Stress associated proteins (SAPs), a class of A20/AN1 zinc finger domain-containing proteins, are involved in a variety of biotic and abiotic stress responses in plants. However, little is known about the SAP gene family and their functions in Tamarix hispida. In this study, we isolated and characterized 11 SAPs from T. hispida. The expression patterns of ThSAPs were analyzed under various stresses (salt and drought) and phytohormone treatment (SA, ABA and MeJA) using real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR). Most ThSAPs exhibited transcriptional responses to abiotic stresses and phytohormones. Among these ThSAPs, ThSAP6 was significantly induced by salt stress. Gain-and loss-of-function analyses revealed that ThSAP6 was a positive regulator of salt stress response. Overexpression of ThSAP6 in T. hispida increased antioxidant enzymes activity and proline content and decreased reactive oxygen species (ROS) accumulation and cell membrane damage under salt stress, while the opposite physiological changes were observed in ThSAP6-RNAi (RNA interference) lines. This study provides a comprehensive description of the SAP gene family in T. hispida, and demonstrates that ThSAP6 is a potential candidate for biotechnological approaches to improve salt tolerance in plants.
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http://dx.doi.org/10.1016/j.plaphy.2021.05.016DOI Listing
May 2021

Antitumor effects of Andrographis via ferroptosis-associated genes in gastric cancer.

Oncol Lett 2021 Jul 12;22(1):523. Epub 2021 May 12.

Department of Gastrointestinal and Pediatric Surgery, Institute of Life Sciences, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan.

The overall prognosis of advanced/metastatic gastric cancer (GC) remains poor despite the development of pharmacotherapy. Therefore, other treatment options, such as complementary and alternative medicine, should be considered to overcome this aggressive malignancy. Andrographis, which is a generally unharmful botanical compound, has gained increasing interest for its anticancer effects in multiple malignancies via the regulation of cancer progression-associated signaling pathways. In the present study, a series of experiments (cell proliferation, colony formation and apoptosis assays) was designed to elucidate the antitumor potential and mechanism of Andrographis in GC cells. The present study demonstrated that Andrographis exerted antitumor effects in GC cell lines (MKN74 and NUGC4) by inhibiting proliferation, reducing colony formation and enhancing apoptotic activity. Furthermore, it was demonstrated that the expression levels of the ferroptosis-associated genes heme oxygenase-1, glutamate-cysteine ligase catalytic and glutamate-cysteine ligase modifier were significantly upregulated after Andrographis treatment in both GC cell lines in reverse transcription-quantitative PCR experiments (P<0.05); this finding was further confirmed by immunoblotting assays (P<0.05). In conclusion, to the best of our knowledge, the present study was the first to demonstrate that Andrographis possessed antitumor properties by altering the expression levels of ferroptosis-associated genes, thereby providing novel insights into the potential of Andrographis as an adjunctive treatment option for patients with metastatic GC.
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http://dx.doi.org/10.3892/ol.2021.12784DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130053PMC
July 2021

Cytidine deaminase can deaminate fused pyrimidine ribonucleosides.

Org Biomol Chem 2021 May 21. Epub 2021 May 21.

Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0358, USA.

The tolerance of cytidine deaminase (CDA) to expanded heterocycles is explored via three fluorescent cytidine analogues, where the pyrimidine core is fused to three distinct five-membered heterocycles at the 5/6 positions. The reaction between CDA and each analogue is followed by absorption and emission spectroscopy, revealing shorter reaction times for all analogues than the native substrate. Pseudo-first order and Michaelis-Menten kinetic analyses provide insight into the enzymatic deamination reactions and assist in drawing comparison to established structure activity relationships. Finally, inhibitor screening modalities are created for each analogue and validated with zebularine and tetrahydrouridine, two known CDA inhibitors.
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http://dx.doi.org/10.1039/d1ob00705jDOI Listing
May 2021

Anti-chronic myeloid leukemia activity and quantitative structure-activity relationship of novel thiazole aminobenzamide derivatives.

Bioorg Med Chem Lett 2021 May 17;44:128116. Epub 2021 May 17.

Institute of Pharmacy and Pharmacology, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang City, PR China; Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, Hengyang City, Hunan Province 421001, PR China. Electronic address:

The anti-chronic myeloid leukemia activity of thiazole aminobenzamide derivatives in vitro was tested by a methanethiosulfonate (MTS)-based viability assay method, and the result showed that some compounds exhibited good inhibitory activities against human chronic myeloid leukemia cell line K562, imatinib-resistant strain K562/R and T135I mutant cell line BaF-ABL-BCR-T315I. Comparative molecular field analysis (CoMFA) and comparative molecular similarity index analysis (CoMSIA) methods were used to analyze the relationship between the structure of thiazole aminobenzamide derivatives and the inhibition of K562/R cell activity. In CoMFA, Q was 0.899 and R was 0.963; in CoMSIA, Q and R were 0.840 and 0.903, respectively. These data indicated that the selected test set showed suitable external predictive ability. Combined with the contour map results, we further analyzed the three-dimensional quantitative structure (3D-QSAR) model. The results demonstrated that in the backbone of the thiazole aminobenzamide derivative, the substitution of a small group at R position, or the introduction of a hydrophilic group at R position, or the introduction of a large-volume amino acid at R position may be beneficial to improve the anti-CML activity of the compound.
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http://dx.doi.org/10.1016/j.bmcl.2021.128116DOI Listing
May 2021

White Light Luminescence from a Homo-conjugated Molecule with Thermally Activated Delayed Fluorescence.

Chem Asian J 2021 May 20. Epub 2021 May 20.

Key Laboratory for Advanced Materials, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China.

Luminophores with tunable emission properties are appealing due to various applications. Among those properties, thermally activated delayed fluorescence (TADF) has been attracting enormous research interests. Herein, we synthesized a 9,9'-spirobifluorene based homo-conjugated molecule 1, which connects a diphenylamino moiety as electron donor and a naphthalimide group as electron acceptor via 2,2'-positions of spirofluorene. Compound 1 displays dual emission behaviour with both blue and orange fluorescence. The one orange fluorescence around 555 nmshows sensitivity to oxygen and a prolonged lifetime of 284 ns in degassed toluene. Such characteristics imply TADF nature for this emission from a charge-transfer excited state. The other emission at 440 nm with blue colour displayed resistance to oxygen quenching and a normal fluorescence lifetime of 1.5 ns. Compared with control molecule, this emission band is assigned as conventional fluorescence from a localized excited state. In addition, dual emission property allows molecule 1 to be modulated to emit white photoluminescence in thin film with a CIE color coordinate of (0.25, 0.33).
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http://dx.doi.org/10.1002/asia.202100397DOI Listing
May 2021

Smart Materials for Dynamic Thermal Radiation Regulation.

Small 2021 May 20:e2100446. Epub 2021 May 20.

Center for Composite Materials and Structure, Harbin Institute of Technology, Harbin, 150001, China.

Thermal radiation in the mid-infrared region profoundly affects human lives in various fields, including thermal management, imaging, sensing, camouflage, and thermography. Due to their fixed emissivities, radiance features of conventional materials are usually proportional to the quadruplicate of surface temperature, which set the limit, that one type of material can only present a single thermal function. Therefore, it is necessary and urgent to design materials for dynamic thermal radiation regulations to fulfill the demands of the age of intelligent machines. Recently, the ability of some smart materials to dynamically regulate thermal radiation has been evaluated. These materials are found to be competent enough for various commands, thereby, providing better alternatives and tremendously promoting the commercial potentials. In this review, the dynamic regulatory mechanisms and recent progress in the evaluation of these smart materials are summarized, including thermochromic materials, electrochromic materials, mechanically and humidity responsive materials, with the potential applications, insufficient problems, and possible strategies highlighted.
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http://dx.doi.org/10.1002/smll.202100446DOI Listing
May 2021

Contribution of WNT2B genetic variants to ischemic stroke occurrence in a Chinese Han population.

J Cardiovasc Pharmacol 2021 Apr 17. Epub 2021 Apr 17.

Department of Anesthesiology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710014, China. Department of Neurology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710014, China.

Abstract: Wnt signaling pathway-related WNT2B gene was up-regulated in ischemic brain damage. We aimed to assess the contribution of WNT2B genetic variant to ischemic stroke (IS) susceptibility in the Chinese Han population. Five polymorphisms including rs3790606, rs351364, rs3790608, rs12037987 and rs10776752 in WNT2B were genotyped using Agena MassARRAY platform in 476 healthy controls and 501 IS patients. Odds ratio (OR) and 95% confidence interval (CI) adjusted for age and gender were estimated by logistic regression analysis. Analysis of Variance was used to evaluate the association between genotypes of WNT2B variants and blood lipid parameters. Rs12037987 (OR = 1.82, 95% CI: 1.18-2.82, p = 0.007) and rs10776752 (OR = 1.74, 95% CI: 1.13-2.68, p = 0.012) were related to the increased IS susceptibility. Interestingly, rs12037987 (OR = 2.01, p = 0.028) and rs10776752 (OR = 2.02, p = 0.028) had the higher IS risk in the subjects aged ≤ 65 years. Rs12037987 (OR = 2.70, p = 0.013), rs10776752 (OR = 2.71, p = 0.012), and rs3790606 (OR = 1.89, p = 0.036) manifested an increasing-risk association with IS occurrence in females. Moreover, rs3790606 genotype was related to serum levels of triglyceride (p = 0.008) and total cholesterol (p = 0.001). Our study reported that rs12037987 and rs10776752 were associated with the increased risk for IS in the Chinese Han population. Our findings may be useful for insight into the contribution of WNT2B variants to the complex pathogenesis of IS.
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http://dx.doi.org/10.1097/FJC.0000000000001032DOI Listing
April 2021

and the Volatile of Attenuate Chronic Myocardial Ischemia Injury in a Pig Model: A Metabonomic Approach for the Mechanism Study.

Oxid Med Cell Longev 2021 28;2021:8840896. Epub 2021 Apr 28.

National Drug Clinical Trial Institute, The Second Affiliated Hospital, Shaanxi University of Chinese Medicine, Xianyang 712000, China.

(SM) coupled with (DO) has been used to relieve cardiovascular diseases in China for many years. Our previous studies have integrated that SM-the volatile oil of DO (SM-DOO)-has a cardioprotective effect on chronic myocardial ischemia based on a pharmacological method, but the cardioprotective mechanism has not been elucidated completely in the metabonomic method. In the present study, a metabonomic method based on high-performance liquid chromatography time-of-flight mass spectrometry (HPLC-Q-TOF-MS) was performed to evaluate the effects of SM-DOO on chronic myocardial ischemia induced by an ameroid constrictor, which was placed on the left anterior descending coronary artery (LAD) of pigs. Pigs were divided into three groups: sham, model, and SM-DOO group. With multivariate analysis, a clear cluster among the different groups was obtained and the potential biomarkers were recognized. These biomarkers were mainly related to energy metabolism, glucose metabolism, and fatty acid metabolism. Furthermore, the protein expressions of phosphorylated AMP-activated protein kinase (p-AMPK) and glucose transporter-4 (GLUT4) were significantly upregulated by SM-DOO. The result indicated that SM-DOO could regulate the above biomarkers and metabolic pathways, especially energy metabolism and glucose metabolism. By analyzing and verifying the biomarkers and metabolic pathways, further understanding of the cardioprotective effect of SM-DOO with its mechanism was evaluated. Metabonomic is a reliable system biology approach for understanding the cardioprotective effects of SM-DOO on chronic myocardial ischemia and elucidating the mechanism underlying this protective effect.
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http://dx.doi.org/10.1155/2021/8840896DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099511PMC
April 2021

LncRNAs induce oxidative stress and spermatogenesis by regulating endoplasmic reticulum genes and pathways.

Aging (Albany NY) 2021 05 6;13(10):13764-13787. Epub 2021 May 6.

Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking University International Hospital, Beijing 102206, China.

Oligozoospermia or low sperm count is a leading cause of male infertility worldwide. Despite decades of work on non-coding RNAs (ncRNAs) as regulators of spermatogenesis, fertilization, and male fertility, the literature on the function of long non-coding RNAs (lncRNAs) in human oligozoospermia is scarce. We integrated lncRNA and mRNA sequencing data from 12 human normozoospermic and oligozoospermic samples and comprehensively analyzed the function of differentially expressed lncRNAs (DE lncRNAs) and mRNAs (DE mRNAs) in male infertility. The target genes of DE lncRNAs were identified using a Gaussian graphical model. Gene ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways were primarily enriched in protein transport and localization to the endoplasmic reticulum (ER). The lncRNA-mRNA co-expression network revealed cis- and trans-regulated target genes of lncRNAs. The transcriptome data implicated DE lncRNAs and DE mRNAs and their target genes in the accumulation of unfolded proteins in sperm ER, PERK-EIF2 pathway-induced ER stress, oxidative stress, and sperm cell apoptosis in individuals with oligozoospermia. These findings suggest that the identified lncRNAs and pathways could serve as effective therapeutic targets for male infertility.
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http://dx.doi.org/10.18632/aging.202971DOI Listing
May 2021

On the association of frustrated Lewis pairs in ionic liquids: a molecular dynamics simulation study.

Phys Chem Chem Phys 2021 Jun 17;23(22):12541-12548. Epub 2021 May 17.

School of Environment and Safety Engineering, North University of China, Taiyuan, 030051, P. R. China.

Sterically hindered frustrated Lewis pairs (FLPs) have the ability to activate hydrogen molecules, and their reactivity is strongly determined by the geometric parameters of the Lewis acids and bases. A recent experimental study showed that ionic liquids (ILs) could largely improve the effective configuration of FLPs. However, the detailed mechanistic profile is still unclear. Herein, we performed molecular dynamics (MD) simulations to reveal the effects of ILs on the structures of FLPs, in particular, the association of Lewis acids and bases. For this purpose, mixed systems were adopted consisting of the ILs [Cmim][NTf] (n = 6, 10, 14), [Cmim][PF] and [Cmim][CTf] and the typical FLP (tBu)P/B(CF) for MD simulations. Radial distribution functions (RDFs) results show that toluene competes with (tBu)P to interact with B(CF), resulting in a relatively low effective (tBu)P/B(CF) complex, while [Cmim][NTf] shows less competition with (tBu)P, which increases the amount of effective FLPs. Spatial distribution functions (SDFs) results show that toluene forms a continuum solvation-shell, which hinders the interactions between (tBu)P and B(CF), while [Cmim][NTf] leaves relatively large empty spaces, which are accessible for (tBu)P or B(CF) molecules, resulting in higher probabilities of effective FLP structures. Lastly, we find that the longer alkyl chain length of [Cmim] cations, the higher the amount of effective (tBu)P/B(CF) pairs, and the anion [CTf] shows negative effects, for which even less effective (tBu)P/B(CF) pairs have been found compared to those of toluene.
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http://dx.doi.org/10.1039/d1cp00479dDOI Listing
June 2021