Publications by authors named "Yao Huang"

405 Publications

Impaired Retrograde Transport Due to Lack of TBC1D5 Contributes to the Trafficking Defect of Lysosomal Cathepsins in Ischemic/Hypoxic Cardiomyocytes.

Front Cardiovasc Med 2021 23;8:796254. Epub 2021 Dec 23.

Department of Wound Repair and Institute of Wound Repair, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, China.

Lysosomal dysfunction has been found in many pathological conditions, and methods to improve lysosomal function have been reported to be protective against infarcted hearts. However, the mechanisms underlying lysosomal dysfunction caused by ischemic injury are far less well-established. The retromer complex is implicated in the trafficking of cation-independent mannose 6-phosphate receptor (CI-MPR), which is an important protein tag for the proper transport of lysosomal contents and therefore is important for the maintenance of lysosomal function. In this study, we found that the function of retrograde transport in cardiomyocytes was impaired with ischemia/hypoxia (I/H) treatment, which resulted in a decrease in CI-MPR and an abnormal distribution of lysosomal cathepsins. I/H treatment caused a reduction in TBC1D5 and a blockade of the Rab7 membrane cycle, which impeded retromer binding to microtubules and motor proteins, resulting in an impairment of retrograde transport and a decrease in CI-MPR. We also established that TBC1D5 was an important regulator of the distribution of lysosomal cathepsins. Our findings shed light on the regulatory role of retromer in ischemic injury and uncover the regulatory mechanism of TBC1D5 over retromer.
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http://dx.doi.org/10.3389/fcvm.2021.796254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8736705PMC
December 2021

Developing a Screening Procedure During the COVID-19 Pandemic: Process and Challenges Faced by a Low-Incidence Area.

Front Med (Lausanne) 2021 24;8:654754. Epub 2021 Dec 24.

Department of Diagnostic Radiology, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

To summarize the imaging results of COVID-19 pneumonia and develop a computerized tomography (CT) screening procedure for patients at our institution with malignant tumors. Following epidemiological investigation, 1,429 patients preparing to undergo anti-tumor-treatment underwent CT scans between February 17 and April 16, 2020. When CT findings showed suspected COVID-19 pneumonia after the supervisor radiologist and the thoracic experience radiologist had double-read the initial CT images, radiologists would report the result to our hospital infection control staff. Further necessary examinations, including the RT-PCR test, in the assigned hospital was strongly recommended for patients with positive CT results. The CT examination room would perform sterilization for 30 min to 1 h. If the negative results of any suspected COVID-19 pneumonia CT findings were identified, the radiologists would upload the results to our Hospital Information Systems and inform clinicians within 2 h. Fifty (0.35%, 50/1,429) suspected pneumonia cases, including 29 males and 21 females (median age: 59.5 years old; age range 27-79 years), were identified. A total of 34.0% (17/50) of the patients had a history of lung cancer and 54.0 (27/50) underwent chemotherapy or targeted therapy. Forty-six patients (92.0%) had prior CT scans, and 35 patients (76.1%) with suspected pneumonia were newly seen (median interval time: 62 days). Sub-pleura small patchy or strip-like lesions most likely due to fibrosis or hypostatic pneumonia and cluster of nodular lesions were the two main signs of suspected cases on CT images (34, 68.0%). Twenty-seven patients (54.0%) had, at least once, follow-up CT scan (median interval time: 18.0 days). Only one patient had an increase in size (interval time: 8 days), the immediately RT-PCR test result was negative. CT may be useful as a screening tool for COVID-19 based on imaging features. But the differential diagnosis between COVID-19 and other pulmonary infection and/or non-infectious disease is very difficult due to its overlapping imaging features.The confirmed diagnosis of the COVID-19 infection should be based on the etiologic eventually. The cancer patients at a low-incidence area would continue treatment by screening carefully before admission.
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http://dx.doi.org/10.3389/fmed.2021.654754DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739482PMC
December 2021

Consensus on the health education of home-based negative pressure wound therapy for patients with chronic wounds: a modified Delphi study.

Burns Trauma 2021 30;9:tkab046. Epub 2021 Dec 30.

Wound Healing Center at Emergency Department, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

Background: The study aimed to develop consensus on the components of health education of home-based negative pressure wound therapy (NPWT) for patients with chronic wounds.

Methods: A Delphi method was used to achieve consensus on the components of health education and 75% agreement and coefficient of variation (CV) <0.25 were used as cutoff. Sixteen experts were recruited purposefully to finish this study.

Results: Two rounds of consultation were implemented. Consensus was achieved on 36 of the 42 statements. The final agreed list of statements represented three domains: health education before carrying out home-based NPWT, health education for the treatment day of NPWT at hospital and health education for NPWT at home.

Conclusions: This study was the first attempt to develop consensus on the comprehensive components of health education of home-based NPWT for patients with chronic wounds. According to the established framework and components of health education, wound professionals can safely and effectively implement health education of home-based NPWT for patients with chronic wounds and improve their self-care ability and treatment experience at home.
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http://dx.doi.org/10.1093/burnst/tkab046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717889PMC
December 2021

The recombinant pseudorabies virus expressing porcine deltacoronavirus spike protein is safe and effective for mice.

BMC Vet Res 2022 Jan 4;18(1):16. Epub 2022 Jan 4.

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.

Background: Porcine deltacoronavirus (PDCoV) is a new pathogenic porcine intestinal coronavirus, which has appeared in many countries since 2012. PDCoV disease caused acute diarrhea, vomiting, dehydration and death in piglets, resulted in significant economic loss to the pig industry. However, there is no commercially available vaccine for PDCoV. In this study, we constructed recombinant pseudorabies virus (rPRVXJ-delgE/gI/TK-S) expressing PDCoV spike (S) protein and evaluated its safety and immunogenicity in mice.

Results: The recombinant strain rPRVXJ-delgE/gI/TK-S obtained by CRISPR/Cas gE gene editing technology and homologous recombination technology has genetic stability in baby hamster syrian kidney-21 (BHK-21) cells and is safe to mice. After immunizing mice with rPRVXJ-delgE/gI/TK-S, the expression levels of IFN-γ and IL-4 in peripheral blood of mice were up-regulated, the proliferation of spleen-specific T lymphocytes and the percentage of CD4 and CD8 lymphocytes in mice spleen was increased. rPRVXJ-delgE/gI/TK-S showed good immunogenicity for mice. On the seventh day after booster immunity, PRV gB and PDCoV S specific antibodies were detected in mice, and the antibody level continued to increase, and the neutralizing antibody level reached the maximum at 28 days post- immunization (dpi). The recombinant strain can protect mice with 100% from the challenge of virulent strain (PRV XJ) and accelerate the detoxification of PDCoV in mice.

Conclusion: The recombinant rPRVXJ-delgE/gI/TK-S strain is safe and effective with strong immunogenicity and is expected to be a candidate vaccine against PDCoV and PRV.
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http://dx.doi.org/10.1186/s12917-021-03115-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8725529PMC
January 2022

Utilization of Melt Fracture Phenomenon for the Preparation of Shark Skin Structured Hydrophobic Film.

Polymers (Basel) 2021 Dec 9;13(24). Epub 2021 Dec 9.

College of Mechanical and Electrical Engineering, Beijing University of Chemical Technology, Beijing 100029, China.

With the application of biomimetic shark skin microstructures with hydrophobicity in microfluidics, sensors and self-cleaning materials, microstructure processing methods are increasing. The preparation process has higher requirements for processing cost and efficiency. In this paper, linear low-density polyethylene (LLDPE) hydrophobic films were prepared with the help of melt fracture phenomenon. The equipment is a self-made single screw extruder. By adjusting the process parameters, the biomimetic shark skin structured LLDPE films with good hydrophobic property can be obtained. The surface microstructure shape of the product is related to kinds of additive, die temperature and screw speed. When AC5 was selected as an additive, the optimal processing parameter was found to be 160 °C die temperature and 80 r/min screw speed. A contact angle of 133° was obtained in this situation. In addition, the influences of die temperature and screw speed on the size of shark skin structure were also systematically investigated in this paper. It was found that the microstructure surface with hierarchical roughness had a better hydrophobic property.
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http://dx.doi.org/10.3390/polym13244299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8705702PMC
December 2021

SIRT1: A Potential Therapeutic Target in Autoimmune Diseases.

Front Immunol 2021 23;12:779177. Epub 2021 Nov 23.

Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China.

The morbidity and mortality of autoimmune diseases (Ads) have been increasing worldwide, and the identification of novel therapeutic strategies for prevention and treatment is urgently needed. Sirtuin 1 (SIRT1), a member of the class III family of nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylases, has been reported to participate in the progression of several diseases. SIRT1 also regulates inflammation, oxidative stress, mitochondrial function, immune responses, cellular differentiation, proliferation and metabolism, and its altered functions are likely involved in Ads. Several inhibitors and activators have been shown to affect the development of Ads. SIRT1 may represent a novel therapeutic target in these diseases, and small molecules or natural products that modulate the functions of SIRT1 are potential therapeutic agents. In the present review, we summarize current studies of the biological functions of SIRT1 and its role in the pathogenesis and treatment of Ads.
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http://dx.doi.org/10.3389/fimmu.2021.779177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650132PMC
November 2021

On-site generation of reactive oxidative radicals from dithionite treated oxic soil slurry.

Environ Technol 2021 Dec 23:1-12. Epub 2021 Dec 23.

State Key Laboratory of Biogeology and Environmental Geology and School of Environmental Studies, China University of Geosciences, Wuhan, People's Republic of China.

Whereas dithionite has been extensively used as a reducing agent in soil and sediment remediation, here, we demonstrate that it can be used as a potential source of oxidizing radical in oxic soils with potential application in organic pollutant remediation. Benzoic acid was used as a probe compound and the generation of its oxidative product para-hydroxybenzoic acid (-HBA) was detected to quantify the production of oxidative radicals (ROS). By increasing the dithionite concentration from 2.5-10 Mm, the accumulated P-HBA concentration in 120 min increased from 15.0-27 µM. Whereas, above 10 mM, the p-HBA concentration decreased due to radical scavenging. Increasing soil dosage from 2.5-15 g/100 mL the accumulated p-HBA amount increased from 22.8-33.7 µM. Temperature 25-35 C and pH 6.2-7.5 were favoured for p-HBA generation. Furthermore, we investigated the roles of different active intermediates in the reaction system and proposed the mechanism behind the ROS genearation. This study suggested that dithionite can be used as an active reagent for advanced oxidation remediation in oxic soil medium.
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http://dx.doi.org/10.1080/09593330.2021.2012267DOI Listing
December 2021

A Hybrid Arm-hand Rehabilitation Robot with EMG-based Admittance Controller.

IEEE Trans Biomed Circuits Syst 2021 Nov 23;PP. Epub 2021 Nov 23.

Reach-and-grasp is one of the most fundamental activities in daily life, while few rehabilitation robots provide integrated and active training of the arm and hand for patients after stroke to improve their mobility. In this study, a novel hybrid arm-hand rehabilitation robot (HAHRR) was built for the reach-and-grasp task. This hybrid structure consisted of a cable-driven module for three-dimensional arm motion and an exoskeleton for hand motion, which enabled assistance of the arm and hand simultaneously. To implement active compliance control, an EMG-based admittance controller was applied to the HAHRR. Experimental results showed that the HAHRR with the EMG-based admittance controller could not only assist the subject in fulfilling the reach-and-grasp task, but also generate smoother trajectories compared with the force-sensing-based admittance controller. These findings also suggested that the proposed approach might be applicable to post-stroke arm-hand rehabilitation training.
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http://dx.doi.org/10.1109/TBCAS.2021.3130090DOI Listing
November 2021

Rheumatic Manifestations and Diseases From Immune Checkpoint Inhibitors in Cancer Immunotherapy.

Front Med (Lausanne) 2021 4;8:762247. Epub 2021 Nov 4.

Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China.

Immune checkpoint inhibitors (ICIs), which can enhance antitumor immunity and inhibit cancer growth, have revolutionized the treatment of multiple cancers and dramatically decreased mortality. However, treatment with ICIs is directly associated with immune-related adverse events (irAEs) because of inflammation in off-target organs and autoimmunity resulting from non-specific immune activation. These irAEs can cause rheumatic diseases and manifestations such as inflammatory arthritis, polymyalgia rheumatica, myositis, vasculitis, Sicca and Sjogen's syndrome, and systemic lupus erythematosus. Early diagnosis and treatment of these adverse events will improve outcomes and quality of life for cancer patients. The treatment of rheumatic diseases induced by ICIs requires multidisciplinary cooperation among physicians. Furthermore, the underlying mechanisms are not fully understood and it is difficult to predict and evaluate these side effects precisely. In this review, we summarize available studies and findings about rheumatic irAEs, focusing mainly on the clinical manifestations, epidemiology, possible mechanisms, and guiding principles for treating these irAEs.
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http://dx.doi.org/10.3389/fmed.2021.762247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599930PMC
November 2021

Development and Validation of a Nomogram for Preoperative Prediction of Localization of Neonatal Gastrointestinal Perforation.

Front Pediatr 2021 2;9:754623. Epub 2021 Nov 2.

Department of Neonatology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China.

Information regarding the localization of gastrointestinal perforation is crucial for the following surgical procedure. This study was to determine the key indicators and develop a prediction model for the localization in neonates with gastrointestinal perforation. A nomogram to predict the location of neonatal gastrointestinal perforation was developed using a cohort of patients who underwent surgery between July 2009 and May 2021. Baseline variables were analyzed using logistics regression and nomogram developed using significant predictors. The predictive performance of the nomogram was assessed by the concordance index (C-index), calibration curve, and area under the receiver operating characteristic (ROC) curve (AUC). The nomogram was further validated in an integrated external cohort. We investigated the data of 201 patients, of which 65 (32.3%) were confirmed with upper gastrointestinal perforation by surgery. Multivariate logistic regression analysis identified the following as independent predictors: preterm [OR: 5.014 (1.492-18.922)], time of onset [OR: 0.705 (0.582-0.829)], preoperative hemoglobin [OR:1.017 (1.001-1.033)], bloody stool: No [OR: 4.860 (1.270-23.588)], shock [OR: 5.790 (1.683-22.455)] and sepsis: No [OR 3.044 (1.124-8.581)]. Furthermore, the nomogram was effective in predicting the perforation site, with an AUC of 0.876 [95% confidence interval (CI): 0.830-0.923]. Internal validation showed that the average AUC was 0.861. Additionally, the model achieved satisfactory discrimination (AUC, 0.900; 95% CI, 0.826-0.974) and calibration (Hosmer-Lemeshow test, = 0.4802) in external validation. The nomogram based on the six factors revealed good discrimination and calibration, suggesting good clinical utility. The nomogram could help surgeons predict the location of gastrointestinal perforation before surgery to make a surgical plan.
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http://dx.doi.org/10.3389/fped.2021.754623DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593177PMC
November 2021

Electroacupuncture for the treatment of perimenopausal syndrome: a systematic review and meta-analysis of randomized controlled trials.

Acupunct Med 2021 Nov 9:9645284211055742. Epub 2021 Nov 9.

Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Objective: To assess the efficacy, comparative effectiveness and safety of electroacupuncture (EA) in the treatment of perimenopausal syndrome (PMS).

Methods: Nine databases were searched until June 2019. Only relevant randomized controlled trials (RCTs) of EA for PMS were included.

Results: Twelve trials involving 746 women were included. EA and hormone therapy (HT) did not significantly differ in terms of effective rate (risk ratio (RR) = 0.98, 95% confidence interval (CI) = 0.93 to 1.04), Kupperman index (KI) (mean difference (MD) = -0.25, 95% CI = -0.76 to 0.26) and serum levels of follicle-stimulating hormone (FSH) (MD = -3.80, 95% CI = -11.59 to 3.98) or luteinizing hormone (LH) (MD = -2.51, 95% CI = -10.72 to 5.70). Serum estradiol (E) levels were significantly lower in EA versus HT groups (MD = -60.58, 95% CI = -71.93 to -49.23). Compared with sham EA, EA had a significantly greater effect on reductions in KI (MD = -4.71, 95% CI = -6.57 to -2.86) and hot flushes score/24 h (MD = -2.43, 95% CI = -2.93 to -1.93). There were no significant differences between EA and manual acupuncture (MA) in terms of effective rate (RR = 1.14, 95% CI = 0.98 to 1.33) or serum FSH (MD = -2.87, 95% CI = -29.65 to 23.91), LH (MD = 2.73, 95% CI = -9.65 to 15.11) or E (MD = 26.80, 95% CI = -12.06 to 65.65). However, it seemed that EA had a better effect than MA on KI (MD = -2.44, 95% CI = -4.80 to -0.08). Subgroup analyses indicated that EA may have more of a benefit in the pre-menopausal state (hot flushes score/24 h: MD = -1.66, 95% CI = -3.49 to 0.17) compared to post-menopause (p > 0.05).

Conclusion: The effect of EA appeared broadly similar to HT and MA in the treatment of PMS, although EA-associated reductions in KI were superior to MA and sham EA, suggesting effects beyond placebo. The evidence base is limited by a small number of eligible studies, risk of bias and clinical/statistical heterogeneity, limiting our ability to draw firm conclusions. As such, additional larger scale, high-quality RCTs are needed.
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http://dx.doi.org/10.1177/09645284211055742DOI Listing
November 2021

TPGS/hyaluronic acid dual-functionalized PLGA nanoparticles delivered through dissolving microneedles for markedly improved chemo-photothermal combined therapy of superficial tumor.

Acta Pharm Sin B 2021 Oct 24;11(10):3297-3309. Epub 2020 Nov 24.

College of Pharmacy, Jinan University, Guangzhou 510632, China.

Nanoparticles (NPs) have shown potential in cancer therapy, while a single administration conferring a satisfactory outcome is still unavailable. To address this issue, the dissolving microneedles (DMNs) were developed to locally deliver functionalized NPs with combined chemotherapy and photothermal therapy (PTT). -Tocopheryl polyethylene glycol succinate (TPGS)/hyaluronic acid (HA) dual-functionalized PLGA NPs (HD10 NPs) were fabricated to co-load paclitaxel and indocyanine green. HD10 NPs significantly enhanced the cytotoxicity of low-dose paclitaxel because of active and mitochondrial targeting by HA and TPGS, respectively. PTT could further sensitize tumor cells toward chemotherapy by promoting apoptosis into the advanced period, highly activating caspase 3 enzyme, and significantly reducing the expression of survivin and MMP-9 proteins. Further, the anti-tumor effects of HD10 NPs delivered through different administration routes were conducted on the 4T1 tumor-bearing mice. After a single administration, HD10 NPs delivered with DMNs showed the best anti-tumor effect when giving chemotherapy alone. As expected, the anti-tumor effect was profoundly enhanced after combined therapy, and complete tumor ablation was achieved in the mice treated with DMNs and intra-tumor injection. Moreover, DMNs showed better safety due to moderate hyperthermia. Therefore, the DMNs along with combined chemo-photothermal therapy provide a viable treatment option for superficial tumors.
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http://dx.doi.org/10.1016/j.apsb.2020.11.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546669PMC
October 2021

Elevated CO Enhances Dynamic Photosynthesis in Rice and Wheat.

Front Plant Sci 2021 1;12:727374. Epub 2021 Oct 1.

Key Laboratory for Earth Surface Processes of Ministry of Education, Department of Ecology, College of Urban and Environmental Sciences, Peking University, Beijing, China.

Crops developed under elevated carbon dioxide (eCO) exhibit enhanced leaf photosynthesis under steady states. However, little is known about the effect of eCO on dynamic photosynthesis and the relative contribution of the short-term (substrate) and long-term (acclimation) effects of eCO. We grew an cultivar and a cultivar under 400 μmol CO mol air (ambient, ) and 600 μmol CO mol air (elevated, ). Regardless of growth [CO], the photosynthetic responses to the sudden increase and decrease in light intensity were characterized under 400 () or 600 μmol CO mol air (). The , , , and treatments were employed to quantify the acclimation effect ( vs. and vs. ) and substrate effect ( vs. and vs. ). In comparison with the treatment, both the steady-state photosynthetic rate ( ) and induction state (IS) were higher under the and treatments but lower under the treatment in both species. However, IS reached at the 60 sec after the increase in light intensity, the time required for photosynthetic induction, and induction efficiency under and treatment did not differ significantly from those under treatment. The substrate effect increased the accumulative carbon gain (ACG) during photosynthetic induction by 45.5% in rice and by 39.3% in wheat, whereas the acclimation effect decreased the ACG by 18.3% in rice but increased it by 7.5% in wheat. Thus, eCO, either during growth or at measurement, enhances the dynamic photosynthetic carbon gain in both crop species. This indicates that photosynthetic carbon loss due to an induction limitation may be reduced in the future, under a high-CO world.
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http://dx.doi.org/10.3389/fpls.2021.727374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517259PMC
October 2021

WTD Attenuating Rheumatoid Arthritis Suppressing Angiogenesis and Modulating the PI3K/AKT/mTOR/HIF-1α Pathway.

Front Pharmacol 2021 27;12:696802. Epub 2021 Sep 27.

Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Wutou Decoction (WTD), as a classic prescription, has been generally used to treat rheumatoid arthritis (RA) for two thousand years in China. However, the potential protective effects of WTD on rheumatoid arthritis and its possible mechanism have rarely been reported. The aim of this study was to explore the possible mechanism of WTD against RA and a promising alternative candidate for RA therapy. A model of collagen-induced arthritis (CIA) was constructed in rats to assess the therapeutic effects of WTD. Histopathological staining, immunofluorescence, and western blotting of synovial sections were conducted to detect the antiangiogenic effects of WTD. Then, cell viability assays, flow cytometry, scratch healing assays, and invasion assays were conducted to explore the effects of WTD on MH7A human fibroblast-like synoviocyte (FLS) cell proliferation, apoptosis, migration, and invasion . The ability of WTD to induce blood vessel formation after MH7A cell and human umbilical vein endothelial cell line (HUVEC) coculture with WTD intervention was detected by a tube formation assay. The mechanisms of WTD were screened by network pharmacology and confirmed by and experiments. WTD ameliorated the symptoms and synovial pannus hyperplasia of CIA rats. Treatment with WTD inhibited MH7A cell proliferation, migration, and invasion and promoted MH7A apoptosis. WTD could inhibit MH7A cell expression of proangiogenic factors, including VEGF and ANGI, to induce HUVEC tube formation. Furthermore, the PI3K-AKT-mTOR-HIF-1α pathway was enriched as a potential target of WTD for the treatment of RA through network pharmacology enrichment analysis. Finally, it was confirmed and that WTD inhibits angiogenesis in RA by interrupting the PI3K-AKT-mTOR-HIF-1α pathway. WTD can inhibit synovial hyperplasia and angiogenesis, presumably by inhibiting the migration and invasion of MH7A cells and blocking the production of proangiogenic effectors in MH7A cells. The possible underlying mechanism by which WTD ameliorates angiogenesis in RA is the PI3K-AKT-mTOR-HIF-1α pathway.
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http://dx.doi.org/10.3389/fphar.2021.696802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502817PMC
September 2021

A novel linear and broadly neutralizing peptide in the SARS-CoV-2 S2 protein for universal vaccine development.

Cell Mol Immunol 2021 11 13;18(11):2563-2565. Epub 2021 Oct 13.

Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, Jiangsu, China.

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http://dx.doi.org/10.1038/s41423-021-00778-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513545PMC
November 2021

Engaging Users on a Q&A Social Media Platform: The Influence of Disease Attributes and Message Features on Public Discussions of Depression.

Front Psychol 2021 24;12:712346. Epub 2021 Sep 24.

School of Journalism and Communication, Renmin University of China, Beijing, China.

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http://dx.doi.org/10.3389/fpsyg.2021.712346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8498105PMC
September 2021

Development of a reverse transcription recombinase-aided amplification assay for detection of Getah virus.

Sci Rep 2021 10 8;11(1):20060. Epub 2021 Oct 8.

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.

GETV, an arbo-borne zoonotic virus of the genus Alphavirus, which causes diarrhea and reproduction disorders in swine, lead to serious economic losses to the swine industry in China. At present, the existing methods for GETV detection are time-consuming and low sensitivity, so, a rapid, accurate and sensitive GETV detection method is urgently needed. In this study, a fluorescent reverse transcription recombinase-assisted amplification method (RT-RAA) was successfully established for the rapid detection of GETV. The sensitivity of this method to GETV was 8 copies/reaction and 20 TCID/reaction. No cross-reaction with other viruses. A total of 118 samples were prepared for GETV detection using fluorescent RT-RAA and SYBR Green I RT-qPCR, the coincidence rate of the two methods was 100%. The results suggest that the RT-RAA method is rapid, sensitive and specific for GETV detection and can be applied in the clinical.
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http://dx.doi.org/10.1038/s41598-021-99734-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501081PMC
October 2021

Digital long-term laser frequency stabilization with an optical frequency comb.

Appl Opt 2021 Jul;60(21):6097-6102

Laser frequency stabilization plays an important role in high-precision spectroscopic measurements. Since high-accuracy commercial wavemeters became available, wavemeter-based frequency stabilization has found a broad application due to its convenience, flexibility, and wide applicability. However, such stabilization schemes frequently suffer from long-term drift, since the accuracy of the wavelength measurement of a wavemeter is affected by ambient temperature fluctuation. In this work, we demonstrate that such long-term drift can be suppressed by regularly calibrating the frequency of a wavemeter-locked laser utilizing an optical frequency comb, which has much better long-term stability. Under this dual-referenced locking scheme, the Allan deviation is reduced to 3.5 E-12 at 4000 s for a fiber laser operated at 548 nm, which when used in the optical Ramsey spectroscopic measurement of , reduces the standard deviation by as much as 40%, compared to the case when only wavemeter locking is applied.
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http://dx.doi.org/10.1364/AO.428587DOI Listing
July 2021

Inpatient Dignity Scale: Mandarin translation and psychometric characteristics evaluation.

Nurs Open 2022 Jan 6;9(1):500-512. Epub 2021 Oct 6.

School of Health Sciences, Caritas Institute of Higher Education, Hong Kong SAR, China.

Aim: The aim of this paper was to translate the English version of the Inpatient Dignity Scale into Mandarin and to test the psychometric properties of the Mandarin version of the Inpatient Dignity Scale.

Design: This research is a cross-sectional survey, using convenience sampling.

Methods: The English version of the Inpatient Dignity Scale was translated into Mandarin Chinese. From June-August 2020, 736 inpatients from 50 tertiary hospitals in Guangzhou were recruited to assess the psychometric attributes of the Inpatient Dignity Scale.

Results: The Mandarin version of the Inpatient Dignity Scale consists of expectation and satisfaction subscales. It differs from the English language version, as the expectation subscale includes three dimensions and the satisfaction subscale includes two dimensions. The total Cronbach alpha coefficient of the expectation subscale and the satisfaction subscale were .820 and .965, respectively, and the split-half reliability of the expectation subscale and the satisfaction subscale were 0.740 and 0.928, respectively, indicating good internal consistency and effectiveness. Known-groups validity was established, as 70% of the hypotheses were supported.
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http://dx.doi.org/10.1002/nop2.1088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8685866PMC
January 2022

Reversible Magnesium Metal Anode Enabled by Cooperative Solvation/Surface Engineering in Carbonate Electrolytes.

Nanomicro Lett 2021 Sep 14;13(1):195. Epub 2021 Sep 14.

School of Materials Science and Engineering, State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou, 310027, Zhejiang, People's Republic of China.

Magnesium metal anode holds great potentials toward future high energy and safe rechargeable magnesium battery technology due to its divalent redox and dendrite-free nature. Electrolytes based on Lewis acid chemistry enable the reversible Mg plating/stripping, while they fail to match most cathode materials toward high-voltage magnesium batteries. Herein, reversible Mg plating/stripping is achieved in conventional carbonate electrolytes enabled by the cooperative solvation/surface engineering. Strongly electronegative Cl from the MgCl additive of electrolyte impairs the Mg…O = C interaction to reduce the Mg desolvation barrier for accelerated redox kinetics, while the Mg-conducting polymer coating on the Mg surface ensures the facile Mg migration and the effective isolation of electrolytes. As a result, reversible plating and stripping of Mg is demonstrated with a low overpotential of 0.7 V up to 2000 cycles. Moreover, benefitting from the wide electrochemical window of carbonate electrolytes, high-voltage (> 2.0 V) rechargeable magnesium batteries are achieved through assembling the electrode couple of Mg metal anode and Prussian blue-based cathodes. The present work provides a cooperative engineering strategy to promote the application of magnesium anode in carbonate electrolytes toward high energy rechargeable batteries.
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http://dx.doi.org/10.1007/s40820-021-00716-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440703PMC
September 2021

The significances and clinical implications of cholesterol components in human breast cancer.

Sci Prog 2021 Jul-Sep;104(3):368504211028395

Department of Surgery, The Eighth Affiliated Hospital, Sun Yat-Sen University, Shenzhen, China.

Breast cancer is one the most common malignancies and leading cause of cancer-related mortality in women. Recent studies suggested that hypercholesterolemia may be the potential modifiable risk factors for breast cancer. Cholesterol was well-known for its strong association with cardiovascular disease for long. Moreover, solid evidence has been provided by different studies to illustrate the correlation between lipid and incidence in multiple cancers. Although the conclusion remains controversial or sometimes contrary, which may be due to the multifactorial nature of the disease and the disparity of ethnic population, it is critical to elucidate the relationship between specific cholesterol components in certain population and the exact underlying mechanism of the lipid-associated signaling pathway in breast cancer. The implications of dysregulated lipoproteins as therapeutic targets or options for breast cancer provide novel strategies for us in combating with this malignant disease, which may be achieved by manipulating lipid levels with pharmacological compounds.
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http://dx.doi.org/10.1177/00368504211028395DOI Listing
September 2021

A comprehensive transcriptomic analysis of alternate interferon signaling pathways in peripheral blood mononuclear cells in rheumatoid arthritis.

Aging (Albany NY) 2021 08 25;13(16):20511-20533. Epub 2021 Aug 25.

Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.

Interferon (IFN) signaling pathways play crucial roles in the pathogenesis of rheumatoid arthritis (RA). Prior studies have mainly studied mixed alterations in the IFN signaling pathway in RA, but these studies have not been sufficient to elucidate how imbalanced IFN signaling subtly influences immune cells. Single-cell RNA (scRNA) sequencing makes it possible to better understand the alternations in the interferon signaling pathways in RA. In the present study, we found that IFN signaling pathways were activated in natural killer (NK) cells, monocytes, T cells, B cells, and most immune cell subclasses in RA. We then explored and analyzed the connections between abnormal IFN signaling pathways and cellular functional changes in RA. Single-Cell rEgulatory Network Inference and Clustering (SCENIC) analysis and gene regulatory network (GRN) construction were also performed to identify key transcription factors in RA. Finally, we also investigated altered IFN signaling pathways in multiple RA peripheral blood samples, which indicated that abnormal IFN signaling pathways were universally observed in RA. Our study contributes to a better understanding of the delicate and precise regulation of IFN signaling in the immune system in RA. Furthermore, common alternations in IFN signaling pathway-related transcription factors could help to identify novel therapeutic targets for RA treatment.
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http://dx.doi.org/10.18632/aging.203432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436925PMC
August 2021

Different Clinicopathologic and Computed Tomography Imaging Characteristics of Primary and Acquired Mutations in Patients with Non-Small-Cell Lung Cancer.

Cancer Manag Res 2021 13;13:6389-6401. Epub 2021 Aug 13.

Department of Diagnostic Radiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.

Purpose: Although patients with primary and acquired epidermal growth factor receptor (EGFR) positive non-small-cell lung cancer (NSCLC) respond to osimertinib treatment, the optimal treatment strategy differs for these two groups of patients. This study aimed to compare the clinicopathologic and computed tomography (CT) imaging characteristics between primary and acquired mutations in patients with NSCLC before treatment.

Patients And Methods: We enrolled two groups of patients with primary or acquired mutation NSCLC (n = 103 per group) from January 2012 to December 2019. We analyzed their clinicopathologic and CT characteristics and differences between the groups. The groups were further categorized based on and to exclude the effect of coexistent mutations.

Results: Primary, compared to acquired, mutation tends to coexist with (P < 0.001), exhibiting earlier tumor stage (P < 0.001), higher differentiation (P = 0.029), higher proportion of lepidic subtype adenocarcinoma (P < 0.001), and significant associations with some CT features (multiple primary lung cancers, ground-glass opacity, air bronchogram, and vacuole sign [all P < 0.001]). The combined model, composed of clinicopathologic and conventional CT signature and CT-radiomic signature, showed good discriminative ability with the area under the receiver operating characteristic curve 0.90 and 0.91 in the training and validation datasets, respectively. The mutation contributed to these differences independently of coexistent mutations.

Conclusion: We identified clinicopathologic and CT imaging differences between primary and acquired mutations. These findings provide insights into developing future personalized mutation status-based theranostic strategies.
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http://dx.doi.org/10.2147/CMAR.S323972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8370596PMC
August 2021

Microtubule associated protein 4 (MAP4) phosphorylation reduces cardiac microvascular density through NLRP3-related pyroptosis.

Cell Death Discov 2021 Aug 12;7(1):213. Epub 2021 Aug 12.

Laboratory Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China.

Phosphorylation of MAP4 (p-MAP4) causes cardiac remodeling, with the cardiac microvascular endothelium being considered a vital mediator of this process. In the current study, we investigated the mechanism underlying p-MAP4 influences on cardiac microvascular density. We firstly confirmed elevated MAP4 phosphorylation in the myocardium of MAP4 knock-in (KI) mice. When compared with the corresponding control group, we detected the decreased expression of CD31, CD34, VEGFA, VEGFR2, ANG2, and TIE2 in the myocardium of MAP4 KI mice, accompanied by a reduced plasma concentration of VEGF. Moreover, we observed apoptosis and mitochondrial disruption in the cardiac microvascular endothelium of MAP4 KI animals. Consistently, we noted a decreased cardiac microvascular density, measured by CD31 and lectin staining, in MAP4 KI mice. To explore the underlying mechanism, we targeted the NLRP3-related pyroptosis and found increased expression of the corresponding proteins, including NLRP3, ASC, mature IL-1β, IL-18, and GSDMD-N in the myocardium of MAP4 KI mice. Furthermore, we utilized a MAP4 (Glu) adenovirus to mimic cellular p-MAP4. After incubating HUVECs with MAP4 (Glu) adenovirus, the angiogenic ability was inhibited, and NLRP3-related pyroptosis were significantly activated. Moreover, both cytotoxicity and PI signal were upregulated by the MAP4 (Glu) adenovirus. Finally, NLRP3 inflammasome blockage alleviated the inhibited angiogenic ability induced by MAP4 (Glu) adenovirus. These results demonstrated that p-MAP4 reduced cardiac microvascular density by activating NLRP3-related pyroptosis in both young and aged mice. We thus managed to provide clues explaining MAP4 phosphorylation-induced cardiac remodeling and enriched current knowledge regarding the role of MAP4.
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http://dx.doi.org/10.1038/s41420-021-00606-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358013PMC
August 2021

Autophagy-Related LC3 Accumulation Interacted Directly With LIR Containing RIPK1 and RIPK3, Stimulating Necroptosis in Hypoxic Cardiomyocytes.

Front Cell Dev Biol 2021 23;9:679637. Epub 2021 Jul 23.

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing, China.

The exact relationships and detailed mechanisms between autophagy and necroptosis remain obscure. Here, we demonstrated the link between accumulated autophagosome and necroptosis by intervening with autophagic flux. We first confirmed that the LC3 interacting region (LIR) domain is present in the protein sequences of RIPK1 and RIPK3. Mutual effects among LC3, RIPK1, and RIPK3 have been identified in myocardium and cardiomyocytes. Direct LC3-RIPK1 and LC3-RIPK3 interactions were confirmed by pull-down assays, and their interactions were deleted after LIR domain mutation. Moreover, after disrupting autophagic flux under normoxia with bafilomycin A1 treatment, or with LC3 or ATG5 overexpression adenovirus, RIPK1, RIPK3, p-RIPK3, and p-MLKL levels increased, suggesting necroptosis activation. Severe disruptions in autophagic flux were observed under hypoxia and bafilomycin A1 co-treated cardiomyocytes and myocardium and led to more significant activation of necroptosis. Conversely, after alleviating hypoxia-induced autophagic flux impairment with LC3 or ATG5 knockdown adenovirus, the effects of hypoxia on RIPK1 and RIPK3 levels were reduced, which resulted in decreased p-RIPK3 and p-MLKL. Furthermore, necroptosis was inhibited by siRNAs against RIPK1 and RIPK3 under hypoxia or normoxia. Based on our results, LIR domain mediated LC3-RIPK1 and LC3-RIPK3 interaction. Besides, autophagosome accumulation under hypoxia lead to necrosome formation and, in turn, necroptosis, while when autophagic flux was uninterrupted, RIPK1 and RIPK3 were cleared through an autophagy-related pathway which inhibited necroptosis. These findings provide novel insights for the role of LC3 in regulating cardiomyocyte necroptosis, indicating its therapeutic potential in the prevention and treatment of hypoxic myocardial injury and other hypoxia-related diseases.
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http://dx.doi.org/10.3389/fcell.2021.679637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8344065PMC
July 2021

Exploring the Clinical Characteristics of COVID-19 Clusters Identified Using Factor Analysis of Mixed Data-Based Cluster Analysis.

Front Med (Lausanne) 2021 16;8:644724. Epub 2021 Jul 16.

Department of Integrated Chinese Traditional and Western Medicine, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China.

The COVID-19 outbreak has brought great challenges to healthcare resources around the world. Patients with COVID-19 exhibit a broad spectrum of clinical characteristics. In this study, the Factor Analysis of Mixed Data (FAMD)-based cluster analysis was applied to demographic information, laboratory indicators at the time of admission, and symptoms presented before admission. Three COVID-19 clusters with distinct clinical features were identified by FAMD-based cluster analysis. The FAMD-based cluster analysis results indicated that the symptoms of COVID-19 were roughly consistent with the laboratory findings of COVID-19 patients. Furthermore, symptoms for mild patients were atypical. Different hospital stay durations and survival differences among the three clusters were also found, and the more severe the clinical characteristics were, the worse the prognosis. Our aims were to describe COVID-19 clusters with different clinical characteristics, and a classifier model according to the results of FAMD-based cluster analysis was constructed to help provide better individualized treatments for numerous COVID-19 patients in the future.
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http://dx.doi.org/10.3389/fmed.2021.644724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8323882PMC
July 2021

Fabrication of chitin nanofiber-PDMS composite aerogels from Pickering emulsion templates with potential application in hydrophobic organic contaminant removal.

J Hazard Mater 2021 10 26;419:126475. Epub 2021 Jun 26.

School of Environmental Studies, China University of Geosciences, 388 Lumo Road, Wuhan 430074, China; State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, China. Electronic address:

Natural polymers have aroused increasing attention in water treatment but their application in removing hydrophobic organic contaminants (HOCs) was limited due to their hydrophilicity. Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated. The Pickering emulsions with PDMS ratios of 2.5-20% v/v showed high stability, demonstrating great potential as aerogel templates. The solidified PDMS droplets were evenly distributed within the matrix, contributing to homogeneous and permanent hydrophobicity. The composite aerogels with water contact angles of over 130° could selectively remove non-aqueous phase HOCs from water. The CCl adsorption capacity was 521-2820 wt%, depending on PDMS contents. Meanwhile, the mechanical resilience of the composite aerogels was significantly improved, facilitating the adsorbent regeneration by simple mechanical squeezing. The adsorption capacity remained above 85% for 24 cycles. Moreover, the aerogels could also remove dissolved HOCs from water with a maximum adsorption capacity of 1.34 mg/g for 10 mg/L TCE. This work reveals the potential of Pickering emulsions in the fabrication of composite hydrophobic materials from natural biopolymers with promising application in HOCs related water treatment.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126475DOI Listing
October 2021

Therapeutic strategies for chronic wound infection.

Chin J Traumatol 2021 Jul 6. Epub 2021 Jul 6.

Wound Healing Unit, Emergency Department, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200125, China. Electronic address:

Chronic wounds have always been a tough fight in clinical practice, which can not only make patients suffer from pain physically and mentally but also impose a heavy burden on the society. More than one factor is relevant to each step of the development of chronic wounds. Along with the in-depth research, we have realized that figuring out the pathophysiological mechanism of chronic wounds is the foundation of treatment, while wound infection is the key point concerned. The cause of infection should be identified and prevented promptly once diagnosed. This paper mainly describes the mechanism, diagnosis and therapeutic strategies of chronic wound infection, and will put an emphasis on the principle of debridement.
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http://dx.doi.org/10.1016/j.cjtee.2021.07.004DOI Listing
July 2021

Dielectric constants of organic pollutants determine their strength for enhancing microbial iron reduction.

Environ Sci Pollut Res Int 2021 Dec 12;28(47):67445-67455. Epub 2021 Jul 12.

State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430078, People's Republic of China.

Physicochemical properties are essential characteristics of organic compounds, which not only impact the fate of organic pollutants but also determine their application in biological processes. Here, we first found that the dielectric constants (ɛ) of organic pollutants negatively correlated to their strength for enhancing microbial Fe(III) reduction. Those with lower ɛ values than 2.61 potentially promoted the above process following the sequence carbon tetrachloride (CT) > benzene > toluene > tetrachloroethylene (PCE) due to their different ability to deprotonate the phosphorus-related groups on the outer cell membrane of iron-reducing bacteria Shewanella oneidensis MR-1 (MR-1). The stronger deprotonation of phosphorus-related groups induced more negative charge of cell surface and more strongly increased cell membrane permeability and consequently stimulated faster release of flavin mononucleotide (FMN) as an electron shuttle/cofactor for Fe(III) reduction. These findings are significant for understanding the biogeochemistry in multi-organic contaminated subsurface and providing knowledge for remediation strategies and current production.
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http://dx.doi.org/10.1007/s11356-021-14060-9DOI Listing
December 2021

Initial boundary value problem for a class of p-Laplacian equations with logarithmic nonlinearity.

Math Biosci Eng 2021 May;18(4):3957-3976

School of Date Science and Information Engineering, Guizhou Minzu University, Guiyang 550025, China.

In this paper, we discuss global existence, boundness, blow-up and extinction properties of solutions for the Dirichlet boundary value problem of the $ p $-Laplacian equations with logarithmic nonlinearity $ u_{t}-{\rm{div}}(|\nabla u|^{p-2}\nabla u)+\beta|u|^{q-2}u = \lambda |u|^{r-2}u\ln{|u|} $, where $ 1 < p < 2 $, $ 1 < q\leq2 $, $ r > 1 $, $ \beta, \lambda > 0 $. Under some appropriate conditions, we obtain the global existence of solutions by means of the Galerkin approximations, then we prove that weak solution is globally bounded and blows up at positive infinity by virtue of potential well theory and the Nehari manifold. Moreover, we obtain the decay estimate and the extinction of solutions.
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http://dx.doi.org/10.3934/mbe.2021198DOI Listing
May 2021
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