Publications by authors named "Yanzheng Gao"

119 Publications

Laminoplasty with selective fusion at unstable segment versus laminectomy with fusion for multilevel cervical myelopathy: a case-control study.

BMC Musculoskelet Disord 2021 May 7;22(1):426. Epub 2021 May 7.

Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, 200011, Shanghai, People's Republic of China.

Background: Segmental cervical instability is a risk factor for the progression of osteophytic bone spurs and development of myelopathy, and is treated as a relative contraindication of cervical laminoplasty. The aim of this study was to compare laminoplasty with selective fixation (LPSF) versus laminectomy with fusion (LCF) in patients with multilevel cervical myelopathy accompanied by segmental instability.

Methods: A case-control study was conducted by reviewing data from 63 patients who underwent LPSF (n = 30) or LCF (n = 33). Cervical alignment, range of motion (ROM), neurologic status and axial symptom severity pre-operation, 3-days after operation, and at the final follow-up (minimum 24 months) were measured and compared between groups.

Results: Postoperation, patients in the LPSF group lost 31.1 ± 17.3 % of cervical lordosis and 43.2 ± 10.9 % cervical ROM while patients in the LCF group lost 5.7 ± 8.2 % and 67.9 ± 15.5 %, respectively. Both LPSF and LCF groups significantly improved neurologic status and axial symptom severity at the final follow-up with similar between-group results(P > 0.05). Blood loss, operation time, hospital stay, and medical cost in the LPSF group were significantly less than in the LCF group(P < 0.05).

Conclusions: In 2 years of clinical observation, LPSF was effective in maintaining the stability of the cervical spine with less sacrifice of mobility and surgical trauma for multilevel myelopathy with segmental instability compared to LCF.
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http://dx.doi.org/10.1186/s12891-021-04297-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105929PMC
May 2021

A meta-analysis of the secondary fractures for osteoporotic vertebral compression fractures after percutaneous vertebroplasty.

Medicine (Baltimore) 2021 Apr;100(16):e25396

Department of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital, Henan Province Intelligent Orthopedic Technology Innovation and Transformation International Joint Laboratory, Henan Key Laboratory for Intelligent Precision Orthopedics, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Henan Zhengzhou.

Abstract: To identify the risk factors of the secondary fractures for osteoporotic vertebral compression fractures (OVCFs) after percutaneous vertebroplasty (PVP).We conducted a search of relevant articles using Cochrane Library, PubMed, Medline, Science Direct, Embase, the Web of Science and other databases. The time range we retrieved from establishment of the electronic database to November 2017. Gray studies were found in the references of included literature reports. STATA version 11.0 (Stata Corporation, College Station, Texas) was used to analyze the pooled data.Fourteen studies involving 1910 patients, 395 of whom had fracture secondary to the surgery were included in this meta-analysis. The result of meta-analyses showed the risk factors of the secondary fractures for OVCFs after PVP was related to bone mineral density (BMD) [95%CI (-0.650, -0.164), SMD=-0.407, P=.001], cement leakage ((RR=0.596, 95%CI (0.444,0.798), P = .001)), and kyphosis after primary operation ((SMD=0.741, 95%CI (0.449,1.032), P = .000)), but not to gender, age, body mass index, cement volume, thoracolumbar spine, and cement injection approaches.Bone mineral density, cement leakage, and kyphosis after primary operation are the risk factors closely correlative to the secondary fracture after PVP. There have not been enough evidences to support the association between the secondary fracture and gender, age, body mass index, cement volume, thoracolumbar spine, and cement injection approaches.
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http://dx.doi.org/10.1097/MD.0000000000025396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078370PMC
April 2021

Biomechanical analysis of a novel clavicular hook plate for the treatment of acromioclavicular joint dislocation: A finite element analysis.

Comput Biol Med 2021 Apr 14;133:104379. Epub 2021 Apr 14.

The Second Department of Orthopedics, Xi'an Centeral Hospital, No. 161, West Fifth Road, Xincheng District, Xi'an, 710003, Shaanxi, China. Electronic address:

Background: Clavicular hook plates are frequently used in clinical orthopedics to treat acromioclavicular joint dislocation. However, patients often exhibit acromial osteolysis and peri-implant fracture after hook plate fixation. To solve the above problems, we developed a novel double-hook clavicular plate and used finite element analysis (FEA) to investigate its biomechanical properties.

Methods: A finite element (FE) model was constructed and validated. Then, a double-hook clavicular plate, a single-hook clavicular plate, and an anatomical double-hook clavicular plate was implanted into the acromioclavicular joint and fixed with screws in groups 1, 2 and 3, respectively. Finally, a load was applied, and some indicators were recorded and analyzed.

Results: For both the proximal clavicular rotation angle and the distal clavicular displacement, the range of motion in groups 1 and 3 was more than 90% lower than that in group 2. The maximum von Mises stress of the clavicle in groups 1 and 3 was more than 45% lower than that in group 2. The maximum stress of the acromion in group 2 was significantly higher than that in groups 1 and 3, and that in group 3 was less than that in group 1, for both cortical and cancellous bone.

Conclusions: The double-hook clavicular plate could immediately reconstruct the stability of the acromioclavicular joint, effectively reducing the stress of the bone around the clavicle and screws. Additionally, the double-hook clavicular plate could reduce the peak stress of the acromion and produce a more uniform stress distribution.
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http://dx.doi.org/10.1016/j.compbiomed.2021.104379DOI Listing
April 2021

Interleukin-1 receptor-associated kinase 4 as a potential biomarker: Overexpression predicts poor prognosis in patients with glioma.

Oncol Lett 2021 Apr 3;21(4):254. Epub 2021 Feb 3.

Department of Orthopaedics, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University, Zhengzhou, Henan 450001, P.R. China.

The undetectable onset of glioma and the difficulty of surgery lead to a poor prognosis. Appropriate biomarkers for diagnosis and treatment need to be identified. Interleukin-1 receptor-associated kinase 4 (IRAK4) is involved in the initiation and progression of cancer. However, up until now, no report has revealed the relationship between IRAK4 and glioma. The present study aimed to examine the expression of IRAK4 in glioma, and to determine if there was a relationship between IRAK4 expression and clinical outcomes or survival prognosis. Thousands of glioma tissue samples and corresponding clinical information were obtained from various databases. Then a series of bioinformatics methods were used to reveal the role of IRAK4 in glioma. Finally, reverse transcription-quantitative PCR technology was used to verify the bioinformatics results. The study found that the expression of IRAK4 was significantly increased in glioma compared with the control brain tissue samples, and IRAK4, as an independent prognostic factor, shortened the overall survival time of patients with glioma. Gene Set Enrichment Analysis showed that IRAK4 promoted the activation of cell signalling pathways, such as NOD-like and Toll-like receptor signalling pathways. Co-expression analysis showed that the expression of IRAK4 was correlated with CMTM6, MOB1A and other genes. The present study demonstrated the role of IRAK4 as an oncogene in the pathological process of glioma for the first time, and highlights the potential of IRAK4 as a biomarker for prognostic evaluation and treatment of glioma.
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http://dx.doi.org/10.3892/ol.2021.12516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882878PMC
April 2021

Abnormal expression of promotes the malignant behavior of glioma cells and leads to poor prognosis of glioma patients.

PeerJ 2021 8;9:e10820. Epub 2021 Feb 8.

Department of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University, Zhengzhou, Henan, China.

Background: Homeobox D11 () plays an important role in a variety of cancers, but its precise role in gliomas remains unclear. This study aimed to explore the relationship between and gliomas by combining bioinformatics methods with basic experimental validation.

Materials And Methods: Obtain gene expression information and clinical information of glioma and non-tumor brain tissue samples from multiple public databases such as TCGA (666 glioma samples), CGGA (749 glioma samples), GEPIA(163 glioblastoma samples and 207 normal control samples), GEO (GSE4290 and GSE15824). Nine cases of glioma tissue and five cases of normal control brain tissue were collected from the clinical department of Henan Provincial People's Hospital for further verification. A series of bioinformatic analysis methods were used to confirm the relationship between expression and overall survival and clinical molecular characteristics of patients with glioma. RT-qPCR was used to verify the change of expression level of in glioma cells and tissues. MTT assay, colony formation assay, wound-healing assay, immunofluorescence staining, flow cytometry and western blotting were used to detect the effect of on the biological behavior of glioma cell line U251.

Results: The high expression of was significantly related to age, World Health Organization (WHO) grade, chemotherapy status, histological type, and even 1p19q codeletion data and isocitrate dehydrogenase (IDH) mutation. , as an independent risk factor, reduces the overall survival of glioma patients and has diagnostic value for the prognosis of glioma. Gene Set Enrichment Analysis (GSEA) showed that was significantly enriched in cell signaling pathway such as cell cycle, DNA replication and so on. Finally, we confirmed that the knockout of can inhibit the proliferation and invasion of U251 glioma cells, and change the biological behavior of tumor cells by preventing the progression of cell cycle.

Conclusions: may be used as a candidate biomarker for the clinical application of targeted drug and prognostic assessment treatment of glioma. In addition, This study will help to explore the pathological mechanism of glioma.
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http://dx.doi.org/10.7717/peerj.10820DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877241PMC
February 2021

Biomechanical properties of a novel nonfusion artificial vertebral body for anterior lumbar vertebra resection and internal fixation.

Sci Rep 2021 Jan 29;11(1):2632. Epub 2021 Jan 29.

Department of Spine and Spinal Cord Surgery, Henan Provincial People's Hospital, People's Hospital of Zhengzhou Umiversity, Zhengzhou, 450003, China.

The aim of the study was to evaluate the biomechanical properties of a novel nonfused artificial vertebral body in treating lumbar diseases and to compare with those of the fusion artificial vertebral body. An intact finite element model of the L1-L5 lumbar spine was constructed and validated. Then, the finite element models of the fusion group and nonfusion group were constructed by replacing the L3 vertebral body and adjacent intervertebral discs with prostheses. For all finite element models, an axial preload of 500 N and another 10 N m imposed on the superior surface of L1. The range of motion and stress peaks in the adjacent discs, endplates, and facet joints were compared among the three groups. The ranges of motion of the L1-2 and L4-5 discs in flexion, extension, left lateral bending, right lateral bending, left rotation and right rotation were greater in the fusion group than those in the intact group and nonfusion group. The fusion group induced the greatest stress peaks in the adjacent discs and adjacent facet joints compared to the intact group and nonfusion group. The nonfused artificial vertebral body could better retain mobility of the surgical site after implantation (3.6°-8.7°), avoid increased mobility and stress of the adjacent discs and facet joints.
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http://dx.doi.org/10.1038/s41598-021-82086-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846776PMC
January 2021

Nano-MoO activates peroxymonosulfate for the degradation of PAH derivatives.

Water Res 2021 Mar 13;192:116834. Epub 2021 Jan 13.

Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

The rapid and efficient degradation of polycyclic aromatic hydrocarbon (PAH) derivatives with toxicological properties remains a substantial challenge. In this study, a cost-effective and eco-friendly catalyst, nano-MoO (0.05 g L), exhibited excellent performance in activating 4.0 mmol L peroxymonosulfate (PMS) for the degradation of naphthalene derivatives with 1 mg L in aqueous systems; these derivatives include 1-methylnaphthalene, 1-nitronaphthalene, 1-chloronaphthalene, 1-naphthylamine and 1-naphthol, with high degradation rates of 87.52%, 86.23%, 97.87%, 99.74%, and 77.16%. Nano-MoO acts as an electron donor by transferring an electron causing O-O bond of PMS to cleave producing SO, and later OH. Electron paramagnetic resonance (EPR) analysis combined with free radical quenching research indicated that SO and OH dominated the degradation of naphthalene derivatives, and O and O participated in the processes. X-ray photoelectron spectroscopy (XPS) revealed the transformation of Mo(IV) to Mo(V) and Mo(VI), which suggested that the activation process proceeded via electron transfer from nano-MoO to PMS. The applicability of the nano-MoO/PMS system in influencing parameters and stability was explored. The degradation pathways were primarily elucidated for each naphthalene derivative based on the intermediates identified in the systems. The -CH, -NO, -Cl, -OH substituents increased the positive electrostatic potential (ESP) on the molecular surface of 1-methylnaphthalene, 1-nitronaphthalene, 1-chloronaphthalene, and 1-naphthol, which reduced the electrophilic reaction and electron transfer between the reactive species and pollutants, leading to a lower degradation rate of naphthalene derivatives than the parent compound. However, the effect of -NH substituents is the opposite. These findings suggest that nano-MoO may aid as a novel catalyst in the future remediation of environments polluted with PAH derivatives.
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http://dx.doi.org/10.1016/j.watres.2021.116834DOI Listing
March 2021

Amino, nitro, chloro, hydroxyl and methyl substitutions may inhibit the binding of PAHs with DNA.

Environ Pollut 2021 Jan 20;268(Pt B):115798. Epub 2020 Oct 20.

Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address:

The binding of PAHs with DNA to form PAH-DNA adducts is a crucial step in PAH-induced carcinogenesis. How functional groups affect this binding is largely unknown. Here, we observed that functional group substitutions strongly inhibited PAH-DNA binding. Additionally, -OH substitution has the most potent inhibitory effect as it causes the smallest change in the electrostatic surface potential. Fourier transform infrared spectroscopy and molecular docking analyses demonstrated that PAH derivatives bind with guanine via intercalation and groove binding and then non-specifically insert into the major/minor grooves of DNA. Quantum chemical calculations suggested that hydrogen/halogen bonding may be essential in affecting the binding of functional group-substituted PAHs with DNA. It was further revealed that Log K and the PAH derivatives' melting points correlated significantly with binding affinity, implying that changes in the physicochemical characteristics are important factors. This study opens a new window for understanding the relationship between highly toxic PAH derivatives and genetic materials.
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http://dx.doi.org/10.1016/j.envpol.2020.115798DOI Listing
January 2021

Whole-cell paper strip biosensors to semi-quantify tetracycline antibiotics in environmental matrices.

Biosens Bioelectron 2020 Nov 27;168:112528. Epub 2020 Aug 27.

Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address:

A novel, low-cost, and portable paper strip biosensor was developed for the detection of tetracycline antibiotics. Escherichia coli/pMTLacZ containing the tetracycline-mediated regulatory gene used as recognition elements with β-galactosidase as the reporter protein was designed and applied to cheap and portable Whatman filter paper as the carrier to prepare this paper strip biosensor. The detection process was optimized by using EDTA and polymyxin B as a sensitizer to improve the accuracy of detection for complicated matrices. The paper strip biosensor was suitable for tetracycline concentrations in the range of 75-10000 μg/L in water and 75-7500 μg/L in soil extracts. Detection limits of 5.23-17.1 μg/L for water and 5.21-35.3 μg/kg for the EDTA soil extracts were achieved at a response time of 90 min. The standard deviation (SD) of detected values by the biosensor paper strip compared to those determined by HPLC was between 13.4 and 59.6% for tetracycline and 2.01-33.5% for oxytetracycline in water and was between 6.22 and 72.8% for tetracycline and 5.90-43.4% for oxytetracycline in soil. This suggests that the paper strip biosensor was suitable for detecting both tetracycline and oxytetracycline in water, and could provide a suitable detection for extractable oxytetracycline in soils. Therefore, this biosensor provides a simple, economical, and portable piece of field kit for on-site monitoring of tetracyclines in a variety of environmental samples, such as pond water and agricultural soil that are susceptible to tetracycline pollution from feed additives and fertilization with livestock manure.
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http://dx.doi.org/10.1016/j.bios.2020.112528DOI Listing
November 2020

Cemented versus uncemented hemiarthroplasty for elderly patients with displaced fracture of the femoral neck: A PRISMA-compliant meta-analysis of randomized controlled trial.

Medicine (Baltimore) 2020 Aug;99(33):e21731

Department of Orthopedics, People's Hospital of Zhengzhou University, Henan Provincial People's Hospital, School of Clinical Medicine, Henan University, Zhengzhou, Henan, China.

Background: This meta-analysis was performed to incorporate newly published, high-quality randomized controlled trials (RCTs) to determine the effects of cemented versus uncemented hemiarthroplasty for elderly patients with displaced fracture of the femoral neck.

Methods: The following electronic databases were extensively searched from the inception of the database through December 2018: EMBASE, Medline, the Cochrane Library, and Web of Science. RCTs focusing on the outcomes of cemented and uncemented hemiarthroplasty were reviewed and screened for eligibility. We used the Cochrane Collaboration's Review Manager Software to perform meta-analyses. Two independent reviewers extracted the data and assessed the study quality and bias risk through the Cochrane Collaboration tool. Use fixed effect model or random effect model to pooled data. Cochran's Q statistic was used to evaluate heterogeneity, and I statistic was used to quantify heterogeneity.

Results: Fifteen RCTs were enrolled (n = 3790) (uncemented hemiarthroplasty group = 1015; cemented hemiarthroplasty group = 1037) (mean age ranged from 70-85.3 years; all patients > 65 years). The meta-analysis showed that cemented hemiarthroplasty has a longer operating time (weighted mean difference, 8.03; 95% confidence interval (CI) 4.83-11.23; P < .00001), less pain (odds ratio, 0.48; 95% CI 4.83-11.23; P = .02), lower mortality 1-year (odds ratio, 0.78; 95% CI 0.62-0.98; P = .03) and fewer implant-related complications (odds ratio, 0.20; 95% CI 0.13-0.30; P < .00001) than Uncemented hemiarthroplasty. However, there are still some limitations in our study, such as the uniformity of the surgery administration programme and rehabilitation scheme, and the small sample size of the included studies.

Conclusions: Cemented hemiarthroplasty for elderly patients with displaced fracture of femoral neck may acquire better functional results.
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http://dx.doi.org/10.1097/MD.0000000000021731DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7437858PMC
August 2020

Effect of Preoperative Zoledronic Acid Administration on Pain Intensity after Percutaneous Vertebroplasty for Osteoporotic Vertebral Compression Fractures.

Pain Res Manag 2020 3;2020:8039671. Epub 2020 Aug 3.

Department of Spinal Cord Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan 45003, China.

Introduction: This study aimed to compare and analyze the effect of preoperative zoledronic acid (ZOL) administration on pain intensity after percutaneous vertebroplasty (PVP) for osteoporotic vertebral compression fracture (OVCF).

Methods: The study included 242 patients with OVCFs who underwent PVP in our hospital between January 2015 and June 2018. The patients were randomly assigned to either a ZOL group ( = 121) or a control group ( = 121). The patients in the ZOL group were treated preoperatively with intravenous infusion of 5 mg ZOL. Those in the control group were treated without ZOL. All the patients were followed up for 1 year.

Results: No statistically significant differences in age, sex, weight, and body mass index (BMI) were found between the two groups. During the follow-up period, the visual analog scale score and Oswestry dysfunction index score in the ZOL group were lower than those in the control group. The bone mineral density at 6 or 12 months after treatment was significantly higher and the levels of the bone metabolism markers were significantly lower in the ZOL group than in the control group ( < 0.05 for both). Two patients in the treatment group had new vertebral fractures, whereas 13 patients in the control group had new vertebral fractures, which translate to recompression vertebral fracture incidence rates of 1.7% and 10.7%, respectively. The incidence rate of mild adverse reactions was significantly higher in the ZOL group than in the control group, but all the cases were endurable.

Conclusion: Intravenous infusion of ZOL before PVP can effectively reduce postoperative pain intensity, reduce bone loss, increase bone density, reduce the risk of refracture, and improve patient quality of life.
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http://dx.doi.org/10.1155/2020/8039671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7421713PMC
January 2021

Biomechanical Comparison of a New Memory Compression Alloy Plate versus Traditional Titanium Plate for Anterior Cervical Discectomy and Fusion: A Finite Element Analysis.

Biomed Res Int 2020 17;2020:5769293. Epub 2020 Jul 17.

Department of Spine and Spinal Cord Surgery, Henan Provincial People's Hospital, 450003, No. 7 the Weft Five Road, Zhengzhou, Henan, China.

Objective: To compare the biomechanical properties of a new memory compression alloy plate and traditional titanium plate after anterior cervical discectomy and fusion (ACDF).

Methods: A finite element model of the C3-7 segments was developed and validated. The C5-6 disc was removed, and an intervertebral cage made of peek material was implanted. Then, a new memory compression alloy plate composed of Ti-Ni memory alloy and a traditional titanium plate were integrated at the C5-6 segment. All models were subjected to a load of 73.6 N to simulate the head weight and 1 Nm of flexion-extension, lateral bending, and axial rotation. The range of segmental motion (ROM) and stress on the prostheses, adjacent discs, and endplates were analyzed.

Results: Compared with intact status, ACDF with the new prothesis and traditional titanium plate reduced the ROM of C5-6 in six directions by 95.2%-100% and increased that of adjacent discs (C4-5 and C6-7) by 4.8%-112.5%. Adjacent disc stress peaks were higher for the traditional titanium plate (0.7-4.2 MPa) than for the new prosthesis (0.6-4.1 MPa). Endplate stress peaks were the highest in ACDF with the new prosthesis (15.6-53.3 MPa), followed by ACDF with traditional titanium plate (5.0-29.4 MPa). Stress peaks were significantly lower for the new prothesis (12.8-52.3 MPa) than for the traditional titanium plate (397.0-666.1 MPa).

Conclusions: The new prosthesis improved the immediate stability of the surgical site and had an elastic modulus that was smaller than that of traditional titanium plate, making it conducive to reducing stress shielding and the impact on the adjacent intervertebral disc.
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http://dx.doi.org/10.1155/2020/5769293DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382732PMC
April 2021

Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis.

Cancer Cell Int 2020 13;20:305. Epub 2020 Jul 13.

Department of Spine and Spinal Cord, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, No. 7, Weiwu Road, Jinshui District, Zhengzhou, 450003 Henan China.

Background: Osteosarcoma (OS) is the most common bone malignant tumor in children, youth, and adolescents. Circular RNA hsa_circ_0005909 (circ_0005909) is involved in the progression of OS. Nevertheless, there are few reports on the role and mechanism of circ_0005909 in OS.

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was executed to examine the expression of circ_0005909, miR-936, and High Mobility Group Box 1 (HMGB1) mRNA in OS tissues and cells. Cell viability, colony formation, migration, and invasion were evaluated by Cell Counting Kit-8 (CCK-8), cell colony formation, or transwell assays. Cell epithelial-mesenchymal transition (EMT) and HMGB1 protein levels were assessed through western blot analysis. The role of circ_0005909 on tumor growth in vivo was verified by xenograft assay. The relationship between circ_0005909 or HMGB1 and miR-936 was confirmed with the dual-luciferase reporter or RNA pull-down assays.

Results: Circ_0005909 level was upregulated in OS tissues and cells. OS patients with high circ_0005909 expression had a lower survival rate. Circ_0005909 inhibition reduced tumor growth in vivo and constrained cell viability, colony formation, migration, invasion, and EMT of OS cells in vitro. Furthermore, circ_0005909 served as a sponge for miR-936 and the repressive impacts of circ_0005909 silencing on malignant behaviors of OS cells were abolished by miR-936 inhibitors. Also, HMGB1 acted as a target for miR-936 and was modulated by circ_0005909 via miR-936. Additionally, HMGB1 overexpression restored the inhibitory influence on the malignant behaviors of OS cells mediated by circ_0005909 inhibition.

Conclusions: Circ_0005909 inhibition impeded the progression of OS via downregulating HMGB1 via sponging miR-936.
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http://dx.doi.org/10.1186/s12935-020-01399-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359231PMC
July 2020

Glomalin-related soil protein reduces the sorption of polycyclic aromatic hydrocarbons by soils.

Chemosphere 2020 Dec 11;260:127603. Epub 2020 Jul 11.

Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

Large amounts of glomalin-related soil protein (GRSP) are present in the soil; however, the impacts of GRSP on the chemical process of soil polycyclic aromatic hydrocarbons (PAHs) are far under investigation. This research sought to elucidate the sorption of phenanthrene as a representative PAH by soils, including Kandiudult, TypicPaleudalf, and Mollisols with co-existing GRSP (0-50 mg/L). The results indicated that soil sorption capacities for phenanthrene reduced significantly. Notably, GRSP changed the sorption process of phenanthrene by Kandiudult, well described as the Freundlich model. In contrast, the phenanthrene sorption isotherms were well described with the Linear model for TypicPaleudalf and Mollisols. The reduced percentage of phenanthrene sorption due to GRSP addition was 7.01%-49.21%, 23.92%-68.71%, and17.26%-66.80% for Kandiudult, TypicPaleudalf and Mollisols, respectively. It was noted that GRSP has a strong capacity for phenanthrene sorption in aqueous solutions and elevates the availability of phenanthrene for microorganisms or plants. During the sorption process, the introduction of GRSP resulted in the reduction of organic matter in soils and elevated the concentrations of dissolved organic matter in solutions, which was the primary mechanism of GRSP-reduced phenanthrene sorption by soils. The findings revealed that GRSP enrichment can increase the mobility of PAHs in contaminated soils.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127603DOI Listing
December 2020

Factors associated with postoperative axial symptom after expansive open-door laminoplasty: retrospective study using multivariable analysis.

Eur Spine J 2020 11 10;29(11):2838-2844. Epub 2020 Jun 10.

Department of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital; People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, 450003, Henan, China.

Purpose: The aim of the present study was to investigate the factors associated with axial symptom using multivariable analysis.

Methods: The authors retrospectively assessed 249 patients treated by open-door laminoplasty. The patients were classified into two groups: axial symptom and no axial symptom group. The possible factors included demographic variables (age, sex, BMI, smoking, heart disease, diabetes, preoperative neck pain, preoperative JOA scores, preoperative NDI, course of disease and pathogenesis) and surgical and radiological variables [operation time, intraoperative blood loss, collar wear time, preoperative cervical curvature, postoperative cervical curvature, T1 slope, preoperative and postoperative C2 sagittal vertical axis (C2 SVA)].

Results: The prevalence of axial symptom was 34.9% (89/249). The collar wear time, preoperative and postoperative C2 SVA were risk factors for axial symptom. A cutoff value of 22.6 mm for preoperative C2 SVA and 3.5 weeks for collar wear time predicted the development of axial symptom.

Conclusions: The longer collar wear time, larger preoperative and postoperative C2 SVA were positively correlated with the higher incidence of axial symptom.
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http://dx.doi.org/10.1007/s00586-020-06494-5DOI Listing
November 2020

LncRNA NR_136400 Suppresses Cell Proliferation and Invasion by Acting as a ceRNA of TUSC5 That Is Modulated by miR-8081 in Osteosarcoma.

Front Pharmacol 2020 15;11:641. Epub 2020 May 15.

Department of Spine Surgery, Changzheng Hospital, The Second Military Medical University, Shanghai, China.

Long non-coding RNAs (lncRNAs) are emerging as important regulators of the processes involved in cancer development and progression. The molecular mechanism by which lncRNAs regulate the progression of osteosarcoma has not been clearly elucidated. The role of NR_136400, which is an uncharacterized lncRNA, has not been previously reported in osteosarcoma (OS). In the present study, we demonstrated that NR_136400 was downregulated in OS cells and that its downregulation promoted OS cell proliferation, apoptosis, and invasion. NR_136400 downregulation facilitated EMT by inhibiting the expression of E-cadherin and elevating the expression of ZEB1, Snail, and fibronectin. experiments using a xenograft tumor mouse model revealed that NR_136400 downregulation promoted tumor growth in OS. Mechanistic investigations demonstrated that NR_136400 competitively bound to miR-8081 and then upregulated the protein expression of TUSC5. Taken together, a newly identified regulatory mechanism of the lncRNA NR_136400/miR-8081/TUSC5 axis was systematically studied in OS, providing a promising target for therapeutic treatment.
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http://dx.doi.org/10.3389/fphar.2020.00641DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242660PMC
May 2020

Abnormally high expression of HOXA2 as an independent factor for poor prognosis in glioma patients.

Cell Cycle 2020 07 21;19(13):1632-1640. Epub 2020 May 21.

Department of Orthopaedics, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University , Zhengzhou, Henan, China.

In recent years, studies have revealed HOXA2 as a new oncogene, but its function is unknown in gliomas. We aimed to reveal the relationship between HOXA2 and glioma based on the Chinese Glioma Genome Atlas(CGGA) and the cancer genome atlas (TCGA). HOXA2 expression data and clinically relevant information of glioma patients were obtained from the CGGA and TCGA containing 1447 glioma tissues and five non-tumor brain tissues. The Wilcox or Kruskal tests were used to detect the correlation between the HOXA2 expression level and clinical data of glioma patients. the Kaplan-Meier method were used to examine the relationship between HOXA2 and overall patient survival. Gene set enrichment analysis (GSEA) was conducted to indirectly reveal the signaling pathways involved in HOXA2, and RT-PCR was used to detect HOXA2 expression in gliomas and non-tumor brain tissues. High HOXA2 expression was found to be positively correlated with clinical grade, histological type, age, and tumor recurrence, but negatively correlated with 1p19 codeletion and isocitrate dehydrogenase mutation status.RT-PCR results showed that HOXA2 expression levels were significantly higher in tumor tissues than in non-tumor brain tissues. GSEA showed that HOXA2 promoted the activation of the activation of the JAK-STAT-signaling pathway, focal adhesion, cell-adhesion-molecules-CAMS pathway, cytosolic DNA sensing pathway, and natural killer cell-mediated cytotoxicity. This study revealed for the first time that the novel oncogene,HOXA2, leads to poor prognosis in gliomas, and can be used as a biomarker for the diagnosis and treatment of gliomas.
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http://dx.doi.org/10.1080/15384101.2020.1762038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469623PMC
July 2020

Antibiotic-contaminated wastewater irrigated vegetables pose resistance selection risks to the gut microbiome.

Environ Pollut 2020 Sep 7;264:114752. Epub 2020 May 7.

Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

Wastewater reuse in food crop irrigation has led to agroecosystem pollution concerns and human health risks. However, there is limited attention on the relationship of sub-lethal antibiotic levels in vegetables and resistance selection. Most risk assessment studies show non-significant toxicity, but overlook the link between antibiotics in crops and propagation of gut microbiome resistance selection. The review highlights the risk of antibiotics in treated water used for irrigation, uptake, and accumulation in edible vegetable parts. Moreover, it elucidates the risks to the adaptive resistance selection of the gut microbiome from sub-lethal antibiotic levels, as a result of dietary contaminated vegetables. Experiments have reported that bacterial resistance selection is possible at concentrations that are several hundred-folds lower than lethal effect levels on susceptible cells. Consequently, mutants selected at low antibiotic levels, such as those from vegetables, are fitter and more resistant compared to those selected at high concentrations. Necessary standardization, such as the development of minimum acceptable antibiotic limits allowable in food crop irrigation water, with a focus on minimum selection concentration, and not only toxicity, has been proposed. Wastewater irrigation offers environmental benefits and can contribute to food security, but it has non-addressed risks. Research gaps, future perspectives, and frameworks of mitigating the potential risks are discussed.
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http://dx.doi.org/10.1016/j.envpol.2020.114752DOI Listing
September 2020

Amino and hydroxy substitution influences pyrene-DNA binding.

Sci Total Environ 2020 Jul 7;725:138542. Epub 2020 Apr 7.

Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

Polycyclic aromatic hydrocarbon (PAH)-DNA binding is an essential step in PAH-induced carcinogenesis. A large number of PAHs contain substituents, it is unclear whether functional groups will influence the PAH-DNA binding. Here, we investigated amino (-NH) and hydroxy (-OH) substitution on pyrene-DNA binding. Because of the considerable effects of electrostatic surface potential (ESP), -NH substitution significantly facilitated binding by increasing the binding constant (log K) from 4.14 L mol to 12.31 L mol, while -OH substitution inhibited binding by reducing log K to 3.68 L mol. Spectroscopy results revealed that pyrene and its derivatives were able to bind with thymine to induce DNA damage or double helix distortion. Quantum chemical calculations showed that -NH substitution induces hydrogen bond formation, thereby enhancing the binding of pyrene with DNA; moreover, binding force changes due to -OH substitution may not be an essential factor. All structural descriptors were not correlated with the quenching constant (K) or binding constant, indicating that changes in physicochemical properties shows no influence on pyrene-DNA binding. The results of this study will improve our understanding of the contribution of functional groups to PAH-DNA binding.
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http://dx.doi.org/10.1016/j.scitotenv.2020.138542DOI Listing
July 2020

The convertion of sewage sludge to biochar as a sustainable tool of PAHs exposure reduction during agricultural utilization of sewage sludges.

J Hazard Mater 2020 06 26;392:122416. Epub 2020 Feb 26.

Department of Environmental Chemistry, Faculty of Chemistry, Maria Curie-Skłodowska University, 3 Maria Curie-Skłodowska Square, 20-031 Lublin, Poland. Electronic address:

There is a discussion in the literature whether PAHs introduced with biochar are safe and whether they are persistent in the environment. The persistence of PAHs (C - total and C - freely dissolved) in sewage sludge (SSL) or SSL-derived biochar-amended soils was investigated. Biochar were produced at 500, 600 and 700 °C. We also compared the persistence of PAHs in these experimental treatments depending on the plants cultivated (grass, clover and thale cress). We showed that the C PAHs in the biochar-amended soils exhibited higher persistence than in the SSL-amended soil. The opposite trend was observed for C PAHs. A higher reduction of C PAHs was noted in the biochar-amended soils than in SSL-amended soil. The persistence of both C and C PAHs clearly varied between the biochars produced at different temperatures. It should be stated that despite that for biochar the persistence of C PAHs is higher compared to SSL-amended soils, an opposite trend is observed for the fraction of C (which is directly responsible for the toxic effect), and this entails a lower risk to the environment (lower mobility and bioavailability). The plants had a significant impact on C PAHs content depending on the number of PAH rings.
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http://dx.doi.org/10.1016/j.jhazmat.2020.122416DOI Listing
June 2020

Lateral Position versus Prone Position for Cervical Laminoplasty: A Retrospective Comparative Study.

Ther Clin Risk Manag 2020 21;16:133-140. Epub 2020 Feb 21.

Microbiome Laboratory, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou City, Henan Province, People's Republic of China.

Purpose: To examine the safety of lateral decubitus positions for cervical laminoplasty.

Patients And Methods: A retrospective comparative study was conducted on the safety between the lateral and prone positions in cervical laminoplasty. After screening, 466 patients who underwent cervical laminoplasty at a single medical center were enrolled and categorized into the lateral (n=229) and prone (n=237) groups. Data on positioning time, surgical time, blood loss, complication rates, and surgical outcomes were collected and compared between the two groups. The patients were further divided into underweight, normal weight, overweight, and obesity subgroups according to their body mass index, and the collected data were compared between the lateral and prone groups.

Results: The lateral group had a lower incidence of facial pressure ulcers (2.18%) than the prone group (11.39%). However, positioning time, surgical time, blood loss, and surgical outcomes were not significantly different between the two groups. In the subgroup analysis, no significant difference in positioning time, operative time, and blood loss was observed in the underweight, normal weight, and overweight patients between the two groups, but in the obesity subgroup, the lateral group had a significantly shorter positioning time (15.23±6.44 vs 21.63±9.43 min, =0.045) and operative time (140.16±40.48 vs 178.62±51.82 min, =0.037) and lesser blood loss (285.31±171.75 vs 430.46±189.84 mL, =0.044) than the prone group.

Conclusion: The lateral position is as safe as the prone position for cervical laminoplasty, but it has advantages over the prone position for patients with obesity.
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http://dx.doi.org/10.2147/TCRM.S227799DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041431PMC
February 2020

Exploring the Key Genes and Pathways in the Formation of Corneal Scar Using Bioinformatics Analysis.

Biomed Res Int 2020 18;2020:6247489. Epub 2020 Jan 18.

Department of Orthopaedics, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University, Zhengzhou, Henan, China.

The Corneal wound healing results in the formation of opaque corneal scar. In fact, millions of people around the world suffer from corneal scars, leading to loss of vision. This study aimed to identify the key changes of gene expression in the formation of opaque corneal scar and provided potential biomarker candidates for clinical treatment and drug target discovery. We downloaded Gene expression dataset GSE6676 from NCBI-GEO, and analyzed the Differentially Expressed Genes (DEGs), Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathway analyses, and protein-protein interaction (PPI) network. A total of 1377 differentially expressed genes were identified and the result of Functional enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) identification and protein-protein interaction (PPI) networks were performed. In total, 7 hub genes IL6 (interleukin-6), MMP9 (matrix metallopeptidase 9), CXCL10 (C-X-C motif chemokine ligand 10), MAPK8 (mitogen-activated protein kinase 8), TLR4 (toll-like receptor 4), HGF (hepatocyte growth factor), EDN1 (endothelin 1) were selected. In conclusion, the DEGS, Hub genes and signal pathways identified in this study can help us understand the molecular mechanism of corneal scar formation and provide candidate targets for the diagnosis and treatment of corneal scar.
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http://dx.doi.org/10.1155/2020/6247489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994212PMC
October 2020

Total hip arthroplasty for intertrochanteric fracture fixation failure.

Eur J Med Res 2019 Dec 27;24(1):39. Epub 2019 Dec 27.

Henan Provincial People's Hospital, Zhengzhou University People's Hospital, 7 Weiwu Road, Zhengzhou, 450003, Henan, People's Republic of China.

Background: Intertrochanteric fracture is a common fracture suffered by elderly patients. Total hip arthroplasty (THA) is regarded as a salvage operation to restore hip joint function after fixation failure, which remains somewhat controversial due to some clinical potential issues.

Methods: 18 elderly patients (average age 70.3 years) each with intertrochanteric fracture fixation failure treated with THA between September 2013 and October 2016 were retrospectively analyzed. Internal fixation treatments involved 5 patients who had received proximal femoral nail anti-rotation, 7 who received locking proximal femur plates and 6 who received dynamic hip screws. All patients were treated with THA using biological acetabular prosthesis and hip arthroplasty (HA) coating skillet femoral prosthesis, with the greater trochanter fixed using wire or steel when necessary. Patients' Harris scores pre- and post-treatment, SF-36 Health Questionnaire score and digital radiology (DR) were used for joint prostheses initial stability and survival evaluation.

Results: 15 patients completed follow-up periods ranging between 19 and 54 months (mean 26.2 months; 1 patient died from a pulmonary embolism, 1 patient died from pulmonary heart disease 1 year after surgery and 1 patient withdrew for personal reasons). There were no joint infections, periprosthetic fractures or dislocations. The average Harris score increased significantly, from 32.68 ± 12.04 points before surgery to 91.08 ± 5.9 points at 24 months post-treatment. SF-36 scores were significantly increased.

Conclusion: THA as salvage treatment for failed internal fixation of intertrochanteric femoral fractures in elderly patients significantly reduced hip pain and restored joint function, and early clinical outcomes were satisfactory.
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http://dx.doi.org/10.1186/s40001-019-0398-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6935170PMC
December 2019

Metabolism of 17β-estradiol by Novosphingobium sp. ES2-1 as probed via HRMS combined with C-labeling.

J Hazard Mater 2020 05 10;389:121875. Epub 2019 Dec 10.

Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China.

This study investigated the biodegradation and metabolic mechanisms of 17β-estradiol (E2) by Novosphingobium sp. ES2-1 isolated from the activated sludge in a domestic sewage treatment plant (STP). It could degrade 97.1% E2 (73.5 μmol/L) in 7 d with a biodegradation half-life of 1.29 d. E2 was initially converted to estrone (E1), then to 4-hydroxyestrone (4-OH-E1), before subsequent monooxygenation reactions cleaved 4-OH-E1 into a metabolite with long-chain ketones structure (metabolite P8). However, when 4-OH-E1 was cleaved through the 4,5-seco pathway, the resulting phenol ring cleavage product could randomly condense with NH to yield a pyridine derivative, accompanied by the uncertain loss of a carboxy group at C4 before the condensation. The derivative was further oxidized into the metabolites with both pyridine and long-chain ketones structure (metabolite N5) through a similar formation mechanism as for P8 performed. This research presents several novel metabolites and shows that E2 can be biodegraded into the metabolite with long-chain structure through three optional pathways, thereby reducing E2 contamination.
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http://dx.doi.org/10.1016/j.jhazmat.2019.121875DOI Listing
May 2020

Platelet-rich plasma vs corticosteroids for elbow epicondylitis: A systematic review and meta-analysis.

Medicine (Baltimore) 2019 Dec;98(51):e18358

Department of Orthopedics, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University.

Objective: The aim of this meta-analysis was to compare the effectiveness of platelet-rich plasma (PRP) vs corticosteroids for treatment of patients with lateral elbow epicondylitis.

Methods: A literature search was performed in EMBASE, Medline, the Cochrane Library and PubMed. Randomized controlled studies comparing PRP with corticosteroids for the treatment of epicondylitis were included. The Cochrane Collaboration's tool for assessing the risk of bias was used to evaluate the methodological quality of the included trials. The Cochrane Collaboration's Review Manager software was used to perform the meta-analyses. The overall effect size of each anesthetic was calculated as the weighted average of the inverse variance of the study-specific estimates.

Results: Seven randomized controlled trials were included in this review. The data from 2 studies were unavailable for meta-analysis, and the systematic review criteria were just achieved. Local corticosteroid injection yielded a significantly superior Disabilities of the Arm, Shoulder and Hand (DASH) score at 4 weeks (WMD, 11.90; 95% CI: 7.72 to 16.08; P < .00001; heterogeneity, χ = 0, I = 0%, P = 1.00) and 8 weeks (WMD, 6.29; 95% CI: 2.98 to 9.60; P = .0002, χ = 0, I = 0%, P = 1.00). Otherwise, it was noteworthy that a significantly lower VAS score (WMD, -2.61; 95% CI: -5.18 to -0.04; P = .05; heterogeneity, χ = 29.85, I = 97%, P < .00001) and DASH score (WMD, -7.73; 95% CI: -9.99 to -5.46; P < .00001, χ = 0.20, I = 0%, P = .66) existed in the PRP regimen than in the steroid regimen at the 24-week follow-up. More effective treatments were achieved in the PRP-treated patients than in the patients treated with corticosteroids (WMD, 3.33; 95% CI: 1.81 to 6.14; P = .000; heterogeneity, χ = 0.43, I = 0%, P = .51).

Conclusions: Local corticosteroid injections demonstrated favorable outcomes compared with those of local PRP treatments for lateral elbow epicondylitis during the short-term follow-up period (4 weeks and 8 weeks post-treatment). Otherwise, at the long-term follow-up (24 weeks post-treatment), PRP injections had improved pain and function more effectively than corticosteroid injections.
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http://dx.doi.org/10.1097/MD.0000000000018358DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940118PMC
December 2019

Occurrence, formation, environmental fate and risks of environmentally persistent free radicals in biochars.

Environ Int 2020 01 15;134:105172. Epub 2019 Nov 15.

Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

Biochars are used globally in agricultural crop production and environmental remediation. However, environmentally persistent free radicals (EPFRs), which are stable emerging pollutants, are generated as a characteristic feature during biomass pyrolysis. EPFRs can induce the formation of reactive oxygen species, which poses huge agro-environmental and human health risks. Their half-lives and persistence in both biochar residues and in the atmosphere may lead to potentially adverse risks in the environment. This review highlights the comprehensive research into these bioreactive radicals, as well as the bottlenecks of biochar production leading up to the formation and persistence of EPFRs. Additionally, a way forward has been proposed, based on two main recommendations. A global joint initiative to create an all-encompassing regulations policy document that will improve both the technological and the quality control aspects of biochars to reduce EPFR generation at the production level. Furthermore, environmental impact and risk assessment studies should be conducted in the extensive applications of biochars in order to protect the environmental and human health. The highlighted key research directions proposed herein will shape the production, research, and adoption aspects of biochars, which will mitigate the considerable concerns raised on EPFRs.
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http://dx.doi.org/10.1016/j.envint.2019.105172DOI Listing
January 2020

A Fast and Easily Parallelizable Biosensor Method for Measuring Extractable Tetracyclines in Soils.

Environ Sci Technol 2020 01 13;54(2):758-767. Epub 2019 Nov 13.

Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences , Nanjing Agricultural University , Nanjing 210095 , China.

Quantification of extractable antibiotics in soils is important to assessing their bioavailability and mobility, and ultimately their ecotoxicological and health risks. This study aimed to establish a biosensor method for detecting extractable tetracyclines in soils (Alfisol, Mollisol, and Ultisol) using whole-cell biosensors containing a reporter plasmid (pMTGFP or pMTmCherry) carrying fluorescent protein genes tightly controlled by tetracyclines-responsive control region (RO). This whole-cell biosensor method can simultaneously measure 96 or more samples within 6 h and is easily parallelizable, whereas a typical high-performance liquid chromatography (HPLC) method may require 7 times more of analysis time and much greater cost to achieve similar analytical throughput. The biosensor method had a detection limit for each of six tetracyclines between 5.32-10.2 μg/kg soil, which is considered adequate for detecting tetracyclines in ethylenediaminetetraacetic acid (EDTA) extracts of soils. Relative standard deviation was between 19.8-51.2% for the biosensor DH5α/pMTGFP and 2.98-25.8% for DH5α/pMTmCherry, respectively, suggesting that DH5α/pMTmCherry was superior to DH5α/pMTGFP for detecting extractable tetracyclines in soils. This new, fast, easily parallelizable, and cost-effective biosensor method has the potential for measuring extractable concentrations of tetracyclines for a large number of soil samples in large-scale monitoring studies.
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http://dx.doi.org/10.1021/acs.est.9b04051DOI Listing
January 2020

Erratum: Angiopoietin-2 induces the neuronal differentiation of mouse embryonic NSCs via phosphatidylinositol 3 kinase-Akt pathway-mediated phosphorylation of mTOR.

Am J Transl Res 2019;11(9):6271-6274. Epub 2019 Sep 15.

Department of Orthopedics, Tianjin Medical University General Hospital No. 154 Anshan Road, Heping District, Tianjin 300052, PR China.

[This corrects the article on p. 1895 in vol. 11, PMID: 30972213.].
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789259PMC
September 2019

Comparison of imaging parameters pre- and post- reductive procedure for atlantoaxial dislocation via posterior fixation using pedicle screw and rod: a cross-sectional study.

BMC Musculoskelet Disord 2019 Oct 15;20(1):451. Epub 2019 Oct 15.

Department of Spinal Surgery, Henan provincial people's hospital, Weiwu road No 7, Jinshui district, Zhengzhou City, Henan province, China.

Background: To compare the imaging parameters pre- and post- reductive procedure for atlantoaxial dislocation via posterior fixation using pedicle screw and rod.

Methods: Thirty-seven patients suffering from atlantoaxial dislocation underwent posterior reduction and internal fixation by pedicle screw and rod. We measured pre-operative and post-operative atlantodental interval (ADI), clivus-canal angle (CCA), cervicomedullary angle (CMA), sum of lateral mass interspace (SLMI) of the operation and the control group. ADI, CCA, CMA, and SLMI between the pre-operative and post-operative conditions of the operation group and the control group were compared.

Results: The ADI, CCA, CMA, and SLMI in the pre-operative condition of the operation group were 8.3 ± 4.3 mm, 130.2 ± 14.2°, 133.8 ± 16.7°, and 3.7 ± 1.3 mm, respectively, those in the post-operative condition of the operation group were 1.0 ± 0.9 mm, 148.5 ± 9.4°, 156.0 ± 8.2°, and 8.0 ± 2.7 mm, respectively, while those in the control group were 1.2 ± 0.3 mm, 152.7 ± 5.3°, 160.2 ± 6.3°, and 4.5 ± 1.0 mm respectively. Post-operative ADI, CCA, CMA, and SLMI were statistically different (p < 0.01) from pre-operative assessments. The SLMI has no significant difference between the pre-operative condition and the control group. Post-operative SLMI was statistically different from that of the control group.

Conclusions: The lateral mass joints were widened after the anatomical reduction of atlantoaxial dislocation by pedicle screw and rod. Widening of the lateral mass exists in both atlantoaxial fusion and occipital-cervical fusion.
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http://dx.doi.org/10.1186/s12891-019-2842-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6794903PMC
October 2019

Glomalin-related soil protein enhances the sorption of polycyclic aromatic hydrocarbons on cation-modified montmorillonite.

Environ Int 2019 11 27;132:105093. Epub 2019 Aug 27.

Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

This study investigated the sorption of phenanthrene (as a representative PAH) by cation-modified montmorillonites (Ca-MMT and Fe-MMT) under the influence of Glomalin-related soil protein (GRSP) fractions (EE-GRSP and T-GRSP). Batch sorption studies were carried out as a function of GRSP concentrations (0-500 mg/L), results suggested that the sorption capacities of Ca-MMT and Fe-MMT for phenanthrene were greatly enhanced. The phenanthrene sorption isotherms were in good agreement with the Linear and Freundlich models (R = 0.886-0.999). The K values increased from 4.14 to 60.76 L/kg for Ca-MMT and from 15.57 to 153.80 L/kg for Fe-MMT with the GRSP concentrations adding from 0 to 500 mg/L, respectively. Furthermore, the sorption of phenanthrene was higher on Fe-MMT than that on Ca-MMT. It is believed that GRSP developed a higher sorption level on Fe-MMT, resulting in higher phenanthrene sorption. Microscopic and Spectroscopic analyses confirmed that the effects of GRSP on phenanthrene sorption were attributed to the changes in the surface structure and the hydrophobic property of montmorillonites. In the sorption process, GRSP may sorb onto montmorillonites through cation-π interaction when a bridge linkage was formed, and phenanthrene bound with GRSP mainly via π-π electron donor-accepter interaction. The findings could provide an in-depth understanding of the ecological functions of GRSP and provide new insights into the pathways of PAH transport and fate in the contaminated fields.
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http://dx.doi.org/10.1016/j.envint.2019.105093DOI Listing
November 2019