Publications by authors named "Yanwei Wang"

187 Publications

Correction to: Well‑Being Reduces COVID‑19 Anxiety: A Three‑Wave Longitudinal Study in China.

J Happiness Stud 2021 Jun 9. Epub 2021 Jun 9.

Beijing Key Laboratory of Applied Experimental Psychology, National Demonstration Center for Experimental Psychology Education (Beijing Normal University), Faculty of Psychology, Beijing Normal University, Beijing, China.

[This corrects the article DOI: 10.1007/s10902-021-00385-2.].
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http://dx.doi.org/10.1007/s10902-021-00406-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188757PMC
June 2021

Epigenetic Regulation of Temperature Responses - Past Successes and Future Challenges.

J Exp Bot 2021 May 29. Epub 2021 May 29.

Beijing Advanced Innovation Centre for Tree Breeding by Molecular Design, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, People's Republic of China.

In contrast to animals, plants cannot avoid unfavorable temperature conditions. Instead, plants have evolved intricate signaling pathways that enable them to perceive and respond to temperature. General acclimation processes that prepare the plant to respond to stressful heat and cold, usually occur throughout the whole plant. More specific temperature responses, however, are limited to certain tissues or cell types. While global responses are amenable to epigenomic analyses, responses which are highly localized are more problematic as the chromatin in question is not easily accessible. Here we review the current knowledge of the epigenetic regulation of FLOWERING LOCUS C and FLOWERING LOCUS T as examples of temperature-responsive flowering time regulators that are expressed broadly throughout the plants and in specific cell types, respectively. While undoubtably extremely successful, we reason that future analyses would benefit from higher spatiotemporal resolution. We conclude by reviewing methods and successful applications of tissue- and cell type-specific epigenomic analyses and provide a brief outlook into the future, single-cell epigenomics.
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http://dx.doi.org/10.1093/jxb/erab248DOI Listing
May 2021

NbN monolayer as a promising anode material for Li/Na/K/Ca-ion batteries: a DFT calculation.

Phys Chem Chem Phys 2021 Jun;23(21):12288-12295

The School of Chemistry and Chemical Engineering, State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, 174 Shazheng Street, Shapingba District, Chongqing City, 400044, P. R. China. and The School of Electrical Engineering, Chongqing University, 174 Shazheng Street, Shapingba District, Chongqing City 400044, P. R. China.

Developing ranking anode materials with sufficient electrical conductivity, ultrafast ion diffusion ability and considerable storage capacity is of great importance for rechargeable ion batteries but still challenging. Herein, using first-principles calculations, the potential of monolayer Nb2N as an anode material for alkali metal (e.g., Li, Na, K and Ca) ion batteries (LIBs, SIBs, PIBs and CIBs) has been explored. The calculated results indicate that the Nb2N monolayer is dynamically and thermally stable with excellent electronic conductivity. To be specific, the Li, Na, K and Ca atoms can be steadily adsorbed on the Nb2N monolayer with a low adsorption energy of -0.996, -1.263, -1.568, and -1.401 eV, respectively. Impressively, the calculated low diffusion barriers for Li, Na, K and Ca on the Nb2N monolayer are 0.047, 0.029, 0.015 and 0.051 eV, respectively, implying its high performance for the ultrafast charge and discharge processes. More importantly, the maximum storage capacities are 536 mA h g-1 for LIBs and 1072 mA h g-1 for CIBs, which are much larger than those of common anode materials. This work not only demonstrates that the Nb2N monolayer can be used as a promising anode material but also inspires the future rational design of other nitride MXenes in energy conversion and storage devices.
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http://dx.doi.org/10.1039/d1cp00993aDOI Listing
June 2021

Identification and validation of miRNA reference genes in poplar under pathogen stress.

Mol Biol Rep 2021 Apr 4;48(4):3357-3366. Epub 2021 May 4.

Beijing Advanced Innovation Center for Tree Breeding By Molecular Design, College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083, China.

Quantitative real time polymerase chain reaction (qRT-PCR) is a common method to analyze gene expression. Due to differences in RNA quantity, quality, and reverse transcription efficiency between qRT-PCR samples, reference genes are used as internal standards to normalize gene expression. However, few universal genes, especially miRNAs, have been identified as reference so far. Therefore, it is essential to identify reference genes that can be used across various experimental conditions, stress treatments, or tissues. In this study, 14 microRNAs (miRNAs) and 5.8S rRNA were assessed for expression stability in poplar trees infected with canker pathogen. Using geNorm, NormFinder and Bestkeeper reference gene analysis programs, we found that miR156g and miR156a exhibited stable expression throughout the infection process. miR156g, miR156a and 5.8S rRNA were then tested as internal standards to measure the expression of miR1447 and miR171c, and the results were compared to small RNA sequencing (RNA-seq) data. We found that when miR156a and 5.8S rRNA were used as the reference gene, the expression of miR1447 and miR171c were consistent with the small RNA-seq expression profiles. Therefore, miR156a was the most stable miRNAs examined in this study, and could be used as a reference gene in poplar under canker pathogen stress, which should enable comprehensive comparisons of miRNAs expression and avoid the bias caused by different length between detected miRNAs and traditional reference genes. The present study has expanded the miRNA reference genes available for gene expression studies in trees under biotic stress.
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http://dx.doi.org/10.1007/s11033-021-06369-yDOI Listing
April 2021

Well-Being Reduces COVID-19 Anxiety: A Three-Wave Longitudinal Study in China.

J Happiness Stud 2021 Mar 27:1-17. Epub 2021 Mar 27.

Beijing Normal University, Beijing, China.

The coronavirus disease 2019 (COVID-19) pandemic threatens human beings' livelihoods and mental health, which lowers their well-being and gives rise to anxiety. This study examines whether there is a causal relationship (and, if so, in which direction) between people's well-being and COVID-19 anxiety. Two hundred and twenty-two participants (54.50% female,  = 31.53,  = 8.17) from 26 provinces of China completed measures of subjective well-being (SWB) and COVID-19 anxiety at three key nodes of the development of COVID-19 in China. The results showed that people's SWB and COVID-19 anxiety fluctuated with the peak (T1), decline (T2), and trough stages (T3) of the COVID-19 pandemic. Meanwhile, the cross-lagged analysis showed that the participants' SWB at T0 (pre-pandemic stage; the base level of SWB) and T1 could significantly predict their COVID-19 anxiety at T1 and T2 respectively. However, SWB at T2 was not associated with the COVID-19 anxiety at T3. Furthermore, COVID-19 anxiety could not predict subsequent SWB from T1 to T3. The current findings contribute to clarifying the causal relationship between well-being and anxiety through the development of epidemics, as well as finding ways to alleviate people's COVID-19 anxiety.

Supplementary Information: The online version contains supplementary material available at 10.1007/s10902-021-00385-2.
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http://dx.doi.org/10.1007/s10902-021-00385-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997794PMC
March 2021

Integration of 3D Hydrodynamic Focused Microreactor with Microfluidic Chemiluminescence Sensing for Online Synthesis and Catalytical Characterization of Gold Nanoparticles.

Sensors (Basel) 2021 Mar 25;21(7). Epub 2021 Mar 25.

Institute of Hydrochemistry, Chair of Analytical Chemistry and Water Chemistry, Technical University of Munich, Elisabeth-Winterhalter-Weg 6, 81377 Munich, Germany.

Chemiluminescence assays have shown great advantages compared with other optical techniques. Gold nanoparticles have drawn much attention in chemiluminescence analysis systems as an enzyme-free catalyst. The catalytic activity of gold nanoparticles for chemiluminescence sensing depends on size, shape and the surface charge property, which is hard to characterize in batches. As there is no positive or negative correlation between chemiluminescence signals and sizes of gold nanoparticles, the best way to get optimal gold nanoparticles is to control the reaction conditions via online chemiluminescence sensing systems. Therefore, a new method was developed for online synthesis of gold nanoparticles with a three-dimension hydrodynamic focusing microreactor, directly coupled with a microfluidic chemiluminescence sensing chip, which was coupled to a charge-coupled device camera for direct catalytical characterization of gold nanoparticles. All operations were performed in an automatic way with a program controlled by Matlab. Gold nanoparticles were synthesized through a single-phase reaction using glucose as a reducing agent and stabilizer at room temperature. The property of gold nanoparticles was easily controlled with the three-dimension microreactor during synthesis. The catalyst property of synthesized gold nanoparticles was characterized in a luminol-NaOCl chemiluminescence system. After optimizing parameters of synthesis, the chemiluminescence signal was enhanced to a factor of 171. The gold nanoparticles synthesized under optimal conditions for the luminol-NaOCl system were stable for at least one month. To further investigate the catalytic activity of synthesized gold nanoparticles in various situations, two methods were used to change the property of gold nanoparticles. After adding a certain amount of salt (NaCl), gold nanoparticles aggregated with a changed surface charge property and the catalytic activity was greatly enhanced. Glutathione was used as an example of molecules with thiol groups which interact with gold nanoparticles and reduce the catalytic activity. The chemiluminescence intensity was reduced by 98.9%. Therefore, we could show that using a microreactor for gold nanoparticles synthesis and direct coupling with microfluidic chemiluminescence sensing offers a promising monitoring method to find the best synthesis condition of gold nanoparticles for catalytic activity.
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http://dx.doi.org/10.3390/s21072290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036713PMC
March 2021

miRNA Mediated Regulation and Interaction between Plants and Pathogens.

Int J Mol Sci 2021 Mar 13;22(6). Epub 2021 Mar 13.

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China.

Plants have evolved diverse molecular mechanisms that enable them to respond to a wide range of pathogens. It has become clear that microRNAs, a class of short single-stranded RNA molecules that regulate gene expression at the transcriptional or post-translational level, play a crucial role in coordinating plant-pathogen interactions. Specifically, miRNAs have been shown to be involved in the regulation of phytohormone signals, reactive oxygen species, and gene expression, thereby modulating the arms race between hosts and pathogens. Adding another level of complexity, it has recently been shown that specific lncRNAs (ceRNAs) can act as decoys that interact with and modulate the activity of miRNAs. Here we review recent findings regarding the roles of miRNA in plant defense, with a focus on the regulatory modes of miRNAs and their possible applications in breeding pathogen-resistance plants including crops and trees. Special emphasis is placed on discussing the role of miRNA in the arms race between hosts and pathogens, and the interaction between disease-related miRNAs and lncRNAs.
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http://dx.doi.org/10.3390/ijms22062913DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999934PMC
March 2021

Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein.

Front Immunol 2021;12:635677. Epub 2021 Mar 11.

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.

The outbreak and worldwide pandemic of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have a significant impact on global economy and human health. In order to reduce the disease spread, 16 monoclonal antibodies (McAbs) again SARS-CoV-2 were generated by immunized mice with the spike protein receptor binding domain (RBD), which was expressed in Chinese hamster ovary cell (CHO). A colloidal gold-based immunochromatographic strip was developed with two McAbs to detect SARS-CoV-2 spike protein, which can play a potential role in monitoring vaccine quality. The strip is highly specific, detecting only SARS-CoV-2 spike protein, and does not show any non-specific reactions with syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV) and other coronavirus and influenza viruses. The strip detected subunit vaccine in our laboratory with a detection limit of spike protein of 62.5 ng/mL. This strip provides an effective method in monitoring vaccine quality by detecting the antigen content of spike protein.
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http://dx.doi.org/10.3389/fimmu.2021.635677DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992422PMC
April 2021

Simple and green synthesis of calcium alginate/AgCl nanocomposites with low-smoke flame-retardant and antimicrobial properties.

Cellulose (Lond) 2021 Mar 22:1-17. Epub 2021 Mar 22.

College of Chemistry and Chemical Engineering, Shandong Collaborative Innovation Center of Marine Biobased Fibers and Ecological Textiles, Qingdao University, Qingdao, 266071 China.

Fire hazards and infectious diseases result in great threats to public safety and human health, thus developing multi-functional materials to deal with these issues is critical and yet has remained challenging to date. In this work, we report a facile and eco-friendly synthetic approach for the preparation of calcium alginate/silver chloride (CA/AgCl) nanocomposites with dual functions of excellent flame-retardant and antibacterial activity. Multi characterization techniques and antibacterial assays were performed to investigate the flame-retardant and antibacterial properties of the CA/AgCl nanocomposites. The obtained results show that the CA/AgCl nanocomposites exhibited much higher limiting oxygen index value (> 60%) than that of CA (48%) with a UL-94 rating of V-0. Moreover, CA/AgCl particularly displayed an efficiently smoke-suppressive feature by achieving a total smoke release value of 2.7 m/m, which was reduced by 91%, compared to CA. The antibacterial rates of the CA/AgCl nanocomposites against E. coli and S. aureus were measured to be 99.67% and 99.77%, respectively, while CA showed quite weak antibacterial rates. In addition, the flame-retardant and antibacterial mechanisms were analyzed and proposed based on the experimental data. This study provides a novel nanocomposite material with both flame-retardant and antibacterial properties which show promising application prospects in the fields of decorative materials and textile industry.
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http://dx.doi.org/10.1007/s10570-021-03825-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982765PMC
March 2021

Retracted: Withaferin-A Inhibits Growth of Drug-Resistant Breast Carcinoma by Inducing Apoptosis and Autophagy, Endogenous Reactive Oxygen Species (ROS) Production, and Inhibition of Cell Migration and Nuclear Factor kappa B (Nf-κB)/Mammalian Target of Rapamycin (m-TOR) Signalling Pathway.

Med Sci Monit 2021 Mar 25;27:e932348. Epub 2021 Mar 25.

Fifth Department of General Surgery, Second Hospital of Lanzhou University, Lanzhou, Gansu, China (mainland).

Retracted, due to breach of publishing guidelines, following the identification of non-original and manipulated figure images. Reference: Xiaokang Liu, Yu Li, Qiang Ma, Yanwei Wang, Ai Lin Song: Withaferin-A Inhibits Growth of Drug-Resistant Breast Carcinoma by Inducing Apoptosis and Autophagy, Endogenous Reactive Oxygen Species (ROS) Production, and Inhibition of Cell Migration and Nuclear Factor kappa B (Nf-kappaB)/Mammalian Target of Rapamycin (m-TOR) Signalling Pathway. Med Sci Monit 2019; 25:6855-6863. 10.12659/MSM.916931.
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http://dx.doi.org/10.12659/MSM.932348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009251PMC
March 2021

Development and characterization of monoclonal antibodies against the N-terminal domain of African swine fever virus structural protein, p54.

Int J Biol Macromol 2021 Jun 16;180:203-211. Epub 2021 Mar 16.

School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China. Electronic address:

African swine fever virus (ASFV), a re-emerging DNA virus, causes a highly contagious disease for domestic pigs. It is running rife worldwide and threatening the global swine industry. Protein p54 is an attractive candidate for ASFV diagnostic and vaccine design. In this work, we designed a peptide to mimic the N-terminal domain (NTD) of ASFV p54 and pretested it with sera from ASFV-infected pigs. The peptide could be well recognized by the sera, implying that the NTD of p54 contained some potential linear B cell epitopes. Then, the conjugates of the peptide with bovine serum albumin were used as the immunogen to generate monoclonal antibodies (mAbs). A total of six mAbs specific to the NTD of ASFV p54 protein were developed. Five of them well reacted with ASFV HLJ/18 strain and recognized a same linear B cell epitope FFQPV. Furthermore, epitope FFQPV could be well recognized by ASFV-positive sera from natural infected pigs, suggesting that it was a natural linear B-cell epitope. Conservation analysis indicated that epitope FFQPV were highly conserved among ASFV epidemic isolates belonging to genotype I and II. Alanine-scanning mutagenesis further revealed that the residues (6F to 9V) of epitope FFQPV were the core binding sites for antibody recognition. This is the first research to characterize specific mAbs against NTD of p54 protein. These findings may help further understand the function of p54 protein and the improvement of ASFV diagnosis.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.03.059DOI Listing
June 2021

The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype O.

Front Vet Sci 2021 25;8:633706. Epub 2021 Feb 25.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.

Foot-and-mouth disease (FMD), caused by FMD virus (FMDV), is a highly contagious and economically devastating viral disease of cloven-hoofed animals worldwide. In this study, the coexpression of small ubiquitin-like modifier (SUMO)-fused capsid proteins of FMDV serotype O by single plasmid in was achieved with an optimal tandem permutation (VP0-VP3-VP1), showing a protein yield close to 1:1:1. After SUMO removal at a low level of protease activity (5 units), the assembled FMDV virus-like particles (VLPs) could expose multiple epitopes and have a size similar to the naive FMDV. Immunization of pigs with the FMDV VLPs could induce FMDV-specific humoral and cellular immune responses effectively, in a dose-dependent manner. These data suggested that the stable FMDV VLPs with multiple epitope exposure were effective for the induction of an immune response in pigs, which laid a foundation for the further development of the FMDV subunit vaccine.
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http://dx.doi.org/10.3389/fvets.2021.633706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947224PMC
February 2021

A porous heterostructure catalyst for oxygen evolution: synergy between IrPnanocrystals and ultrathin P,N-codoped carbon nanosheets.

Nanotechnology 2021 Mar 8. Epub 2021 Mar 8.

Chongqing Univ., Chongqing, CHINA.

Designing efficient and robust oxygen evolution reaction (OER) electrocatalysts is of great importance for various electrochemical energy storage and conversion applications. Herein, we developed IrP2 nanocrystals uniformly anchored in P,N-codoped carbon nanosheets ([email protected]) as highly active OER electrocatalysts. The ultrathin PNC-NS reconstructs an agaric-like porous structure, which can inhibit the agglomeration of the IrP2 nanocrystals effectively. Moreover, the in-situ phosphatization leads to the formation of a strong electron interaction between PNC-NS and IrP2 nanocrystals, endowing the heterostructure materials with satisfying synergistic effects. Benefiting from the collaborative advantages of ideal configuration structure and favorable synergistic effects, [email protected] exhibits excellent OER performance with a low overpotential of 221 mV at 10 mA cm-2, and a small Tafel slope of 37.5 mV dec-1. DFT calculations reveal that the synergistic effects derived from the IrP2/PNC interface, which can effectively tune the activation barriers towards facilitating the oxygen evolution process. This work provides a new insight into the design of heterostructure materials for advanced OER electrocatalysts.
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http://dx.doi.org/10.1088/1361-6528/abecb6DOI Listing
March 2021

Novel Application of Nanofluidic Chip Digital PCR for Detection of African Swine Fever Virus.

Front Vet Sci 2020 5;7:621840. Epub 2021 Feb 5.

School of Life Sciences, Zhengzhou University, Zhengzhou, China.

African swine fever virus (ASFV) gives rise to a grievous transboundary and infectious disease, African swine fever (ASF), which has caused a great economic loss in the swine industry. To prevent and control ASF, once suspicious symptoms have presented, the movement of animal and pork products should be stopped, and then, laboratory testing should be adopted to diagnose ASF. A method for ASFV DNA quantification is presented in this research, which utilizes the next-generation PCR platform, nanofluidic chip digital PCR (cdPCR). The cdPCR detection showed good linearity and repeatability. The limit of detection for cdPCR is 30.1995 copies per reaction, whereas no non-specific amplification curve was found with other swine viruses. In the detection of 69 clinical samples, the cdPCR showed significant consistency [91.30% (63/69)] to the Office International des Epizooties-approved quantitative PCR. Compared with the commercial quantitative PCR kit, the sensitivity of the cdPCR assay was 86.27% (44/50), and the specificity was 94.44% (17/18). The positive coincidence rate of the cdPCR assay was 88% (44/50). The total coincidence rate of the cdPCR and kit was 89.86% (62/69), and the kappa value reached 0.800 ( < 0.0001). This is the first time that cdPCR has been applied to detecting ASFV successfully.
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http://dx.doi.org/10.3389/fvets.2020.621840DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894257PMC
February 2021

Theoretical screening of VSe as support for enhanced electrocatalytic performance of transition-metal single atoms.

J Colloid Interface Sci 2021 May 26;590:210-218. Epub 2021 Jan 26.

The School of Chemistry and Chemical Engineering, State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, 174 Shazheng Street, Shapingba District, Chongqing City 400044, PR China; The School of Electrical Engineering, Chongqing University, 174 Shazheng Street, Shapingba District, Chongqing City 400044, PR China. Electronic address:

Single-atom catalysts (SACs) have attracted enormous attentions in heterogeneous catalysts due to the maximized atomic utilization and extraordinary catalytic performance. Similar to homogeneous catalytic ligands, the support in SACs plays a vital role in the catalytic properties. Herein, we present a series of transition-metal atoms (Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ru, Rh, Pd, Os, Ir and Pt) anchored on a vanadium diselenide (VSe) monolayer as electrocatalysts through density functional theory calculations. [email protected] stands out among the considered SACs with a low overpotential of 0.38 V, exhibiting the excellent performance of oxygen reduction reaction (ORR). Meanwhile, a liner trend between the adsorption Gibbs free energy of the OH (ΔG*) and the predicted η is revealed, which may serve as a simple descriptor for the inherent ORR catalytic activity of SACs. Particularly, [email protected] shows extraordinarily low theoretical overpotential of -0.04/0.47 V for hydrogen/oxygen evolution reaction, which transcends the state-of-the-art Pt and IrO and thereby can be exploited as highly-efficient bifunctional electrocatalyst for overall water splitting. This work broadens the perception of designing multifunctional electrocatalysts based on two-dimensional VSe material and offers a new paradigm for investigating advanced SACs.
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http://dx.doi.org/10.1016/j.jcis.2021.01.062DOI Listing
May 2021

Anti-PD1/PD-L1 monotherapy vs standard of care in patients with recurrent or metastatic head and neck squamous cell carcinoma: A meta-analysis of randomized controlled trials.

Medicine (Baltimore) 2021 Jan;100(4):e24339

Central Laboratory, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.

Background: In recent years, a number of clinical trials for antibody drugs targeting programmed cell death protein 1 (PD1)/programmed cell death 1 ligand 1 (PD-L1) have been carried out on recurrent or metastatic head and neck squamous cell carcinoma (R/M SCCHN) and reported promising prospects. To further evaluate and understand the effects and risk of anti-PD1/PD-L1 monotherapy vs standard of care (SoC) in R/M SCCHN, we conducted this meta-analysis of published randomized controlled trials.

Method: The potential eligible trials were searched from Cochrane library and Pubmed and Embase databases. Randomized controlled trials evaluating the effects and risk of anti-PD1/PD-L1 monotherapy vs SoC in platinum refractory R/M SCCHN were selected. The outcomes, including objective response rate, disease control rate, progression-free survival, overall survival, and treatment-related adverse events, were extracted and pooled.

Results: 1345 patients with R/M SCCHN from three randomized controlled trials were enrolled in this analysis. Compared with SoC, anti-PD1/PD-L1 monotherapy could provide statistically significant overall survival benefit, hazard ratio (95% confidence interval ) = 0.79 [0.70-0.90]. However, we observed no significant difference between 2 treatments in progression-free survival (hazard ratio [95% confidence interval] = 0.96 [0.85-1.09]). Furthermore, anti-PD1/PD-L1 monotherapy caused less treatment-related adverse events than standard of care.

Conclusion: Anti-PD1/PD-L1 monotherapy could indeed reduce the risk of death in R/M SCCHN patients, and provide higher safety vs SoC. Expression level of PD-L1 may be a useful biomarker for selecting patients with better response to anti-PD1/PD-L1 monotherapy.
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http://dx.doi.org/10.1097/MD.0000000000024339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7850637PMC
January 2021

Reasonable permutation of M2e enhances the effect of universal influenza nanovaccine.

Int J Biol Macromol 2021 Mar 21;173:244-250. Epub 2021 Jan 21.

School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China. Electronic address:

Influenza A virus (IAV) occasionally cross-species transmission among humans, swine and avian. The ectodomain of matrix protein 2 (M2e) is highly conserved in IAV, and multi-copy M2e from different species are usually displayed on the surface of nanoparticles to improve immunogenicity and constitute universal IAV nanovaccines. In our previous study, three M2e were inserted into the C-terminal of Cap protein of porcine circovirus type 2 (PCV2) to form a universal nanovaccine that provides protection against PCV2 and different subtypes of IAV. However, M2e adopts at least two converted conformations, and the intermolecular linker of M2e enhances the conformational instability, which limits the recognition by B cell receptors and production of high-level antibodies. Here, we report that the permutation of M2e affects effectiveness of nanovaccines. Three M2e derived from humans, swine and avian IAV were inserted into the C-terminal of Cap protein to form nanovaccines. Immunoprotective effects of different M2e arrangements were explored in mice. Results showed that the M2e closest to the surface of nanoparticle induced the most efficient protection against IAV derived from corresponding species. The results will contribute to develop more effective PCV2 and universal IAV bivalent nanovaccines for pigs, as well as species-specific universal IAV vaccines.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.01.132DOI Listing
March 2021

Identifying breast cancer subtypes associated modules and biomarkers by integrated bioinformatics analysis.

Biosci Rep 2021 Jan;41(1)

Department of General Surgery, Lanzhou University Second Hospital, Lanzhou 730030, Gansu, People's Republic of China.

Breast cancer is the most common form of cancer afflicting women worldwide. Patients with breast cancer of different molecular classifications need varied treatments. Since it is known that the development of breast cancer involves multiple genes and functions, identification of functional gene modules (clusters of the functionally related genes) is indispensable as opposed to isolated genes, in order to investigate their relationship derived from the gene co-expression analysis. In total, 6315 differentially expressed genes (DEGs) were recognized and subjected to the co-expression analysis. Seven modules were screened out. The blue and turquoise modules have been selected from the module trait association analysis since the genes in these two modules are significantly correlated with the breast cancer subtypes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment show that the blue module genes engaged in cell cycle, DNA replication, p53 signaling pathway, and pathway in cancer. According to the connectivity analysis and survival analysis, 8 out of 96 hub genes were filtered and have shown the highest expression in basal-like breast cancer. Furthermore, the hub genes were validated by the external datasets and quantitative real-time PCR (qRT-PCR). In summary, hub genes of Cyclin E1 (CCNE1), Centromere Protein N (CENPN), Checkpoint kinase 1 (CHEK1), Polo-like kinase 1 (PLK1), DNA replication and sister chromatid cohesion 1 (DSCC1), Family with sequence similarity 64, member A (FAM64A), Ubiquitin Conjugating Enzyme E2 C (UBE2C) and Ubiquitin Conjugating Enzyme E2 T (UBE2T) may serve as the prognostic markers for different subtypes of breast cancer.
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http://dx.doi.org/10.1042/BSR20203200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796196PMC
January 2021

Enrichment of waste sewage sludge for enhancing methane production from cellulose.

Bioresour Technol 2021 Feb 3;321:124497. Epub 2020 Dec 3.

Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu, Kitakyushu 808-0196, Japan. Electronic address:

Low ability of waste sewage sludge to degrade cellulose is observed due to its less cellulolytic bacteria content. The enrichment of sewage sludge in the absence or presence of carboxymethylcellulose (CMC) was conducted to improve anaerobic digestion (AD) of cellulose in this study. Compared to initial sewage sludge (IS), enriched sludge without CMC addition (ES) displayed 69.81% higher CH yield and about 1.7-fold greater anaerobic biodegradation of cellulose. In particular, bacterial and archaeal diversities in samples inoculated with ES were significantly altered, with Ruminiclostridium and Methanobacterium as the predominant genera. Enriched sludge with CMC addition (ESC) displayed enhanced methane production at initial cellulose fermentation but showed no distinct difference compared with the control after incubation 24 days. These findings suggest that enrichment of waste sewage sludge without CMC addition is more beneficial for promoting AD of cellulose, providing a novel insight for efficient energy utilization of lignocellulosic wastes.
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http://dx.doi.org/10.1016/j.biortech.2020.124497DOI Listing
February 2021

Lead I R-wave amplitude to distinguish ventricular arrhythmias with lead V transition originating from the left versus right ventricular outflow tract.

Clin Cardiol 2021 Jan 10;44(1):100-107. Epub 2020 Dec 10.

Department of Cardiology, Second Hospital of Hebei Medical University, Shijiazhuang, China.

Background: The electrophysiology algorithm for localizing left or right origins of outflow tract ventricular arrhythmias (OT-VAs) with lead V transition still needs further investigation in clinical practice.

Hypothesis: Lead I R-wave amplitude is effective in distinguishing the left or right origin of OT-VAs with lead V transition.

Methods: We measured lead I R-wave amplitude in 82 OT-VA patients with lead V transition and a positive complex in lead I who underwent successful catheter ablation from the right ventricular outflow tract (RVOT) and left ventricular outflow tract (LVOT). The optimal R-wave threshold was identified, compared with the V S/V R index, transitional zone (TZ) index, and V transition ratio, and validated in a prospective cohort study.

Results: Lead I R-wave amplitude for LVOT origins was significantly higher than that for RVOT origins (0.55 ± 0.13 vs. 0.32 ± 0.15 mV; p < .001). The area under the curve (AUC) for lead I R-wave amplitude as assessed by receiver operating characteristic (ROC) analysis was 0.926, with a cutoff value of ≥0.45 predicting LVOT origin with 92.9% sensitivity and 88.2% specificity, superior to the V S/V R index, TZ index, and V transition ratio. VAs in the LVOT group mainly originated from the right coronary cusp (RCC) and left and right coronary cusp junction (L-RCC). In the prospective study, lead I R-wave amplitude identified the LVOT origin with 92.3% accuracy.

Conclusion: Lead I R-wave amplitude provides a useful and simple criterion to identify RCC or L-RCC origin in OT-VAs with lead V transition.
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http://dx.doi.org/10.1002/clc.23511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803356PMC
January 2021

[Co-expression, purification and bioassay of three avian viral antigens].

Sheng Wu Gong Cheng Xue Bao 2020 Oct;36(10):2066-2075

National Research Center for Veterinary Medicine, Luoyang 471000, Henan, China.

To achieve uniform soluble expression of multiple proteins in the same Escherichia coli strain, and simplify the process steps of antigen production in genetic engineering subunit multivalent vaccine, we co-expressed three avian virus proteins including the fowl adenovirus serotype 4 (FAdV-4) Fiber-2 protein, infectious bursal disease virus (IBDV) VP2 protein and egg-drop syndrome virus (EDSV) Fiber protein in E. coli BL21(DE3) cells after optimization of gene codon, promoter, and tandem expression order. The purified proteins were analyzed by Western blotting and agar gel precipitation (AGP). The content of the three proteins were well-proportioned after co-expression and the purity of the purified proteins were more than 80%. Western blotting analysis and AGP experiment results show that all the three co-expression proteins had immunoreactivity and antigenicity. It is the first time to achieve the three different avian virus antigens co-expression and co-purification, which simplified the process of antigen production and laid a foundation for the development of genetic engineering subunit multivalent vaccine.
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http://dx.doi.org/10.13345/j.cjb.200057DOI Listing
October 2020

Cobalt-catalyzed carboxylation of aryl and vinyl chlorides with CO.

Chem Commun (Camb) 2020 Nov;56(92):14416-14419

State Key Laboratory of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin 300071, P. R. China.

The transition-metal-catalyzed carboxylation of aryl and vinyl chlorides with CO2 is rarely studied, and has been achieved only with a Ni catalyst or combination of palladium and photoredox. In this work, the cobalt-catalyzed carboxylation of aryl and vinyl chlorides and bromides with CO2 has been developed. These transformations proceed under mild conditions and exhibit a broad substrate scope, affording the corresponding carboxylic acids in good to high yields.
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http://dx.doi.org/10.1039/d0cc06451cDOI Listing
November 2020

Non-Metal Single-Phosphorus-Atom Catalysis of Hydrogen Evolution.

Angew Chem Int Ed Engl 2020 Dec 22;59(52):23791-23799. Epub 2020 Oct 22.

The School of Chemistry and Chemical Engineering, State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, 174 Shazheng Street, Shapingba District, Chongqing City, 400044, P. R. China.

Non-metal-based single-atom catalysts (SACs) offer low cost, simple synthesis methods, and effective regulation for substrates. Herein, we developed a simplified pressurized gas-assisted process, and report the first non-metal single-atom phosphorus with atomic-level dispersion on unique single-crystal Mo C hexagonal nanosheet arrays with a (001) plane supported by carbon sheet (SAP-Mo C-CS). The SAP-Mo C-CS is structurally stable and shows exceptional electrocatalytic activity for the hydrogen evolution reaction (HER). A so-called high-active "window" based on the active sites of P atoms and their adjacent Mo atoms gives a ΔG close to zero for hydrogen evolution, which is the most ideal ΔG reported so far. Meanwhile, the moderate d-band center value of SAP-Mo C-CS can be also used as an ideal standard value to evaluate the HER performance in non-metal-based SACs.
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http://dx.doi.org/10.1002/anie.202011358DOI Listing
December 2020

Dietary cadmium exposure causes elevation of blood ApoE with triglyceride level in mice.

Biometals 2020 10 15;33(4-5):241-254. Epub 2020 Sep 15.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212000, Jiangsu, China.

Cadmium (Cd) is a widespread toxic occupational and environmental pollutant, and its effect on lipid metabolism dysregulation has been observed. In this study, we utilized two-dimensional electrophoresis (2-DE) and mass spectrometry (MS) technologies to explore changes in the blood plasma proteins of mice exposed to Cd. From the 2-DE, 8 protein spots were screened in response to Cd exposure, and the identities of these proteins were revealed by MALDI-TOF MS. Western blotting was applied to analyze the expression of the apolipoproteins in both plasma and liver, which were consistent with Cd-induced dyslipidemia of their composed lipid. Moreover, the Cd-induced apolipoprotein ApoE upregulation was due to inhibition of autopahgic flux in the Cd exposed mice. It was further observed from the mouse liver that Cd reduced the expression of the lipid uptake receptor low-density lipoprotein receptor (LDLR), which might be responsible for the coordinated elevation in blood triglycerides and abnormal apolipoproteins. This study may provide a new insight into the mechanism of Cd-induced dyslipidemia and the risk of cardiovascular diseases.
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http://dx.doi.org/10.1007/s10534-020-00247-zDOI Listing
October 2020

Nephrotoxicity Profile of Cadmium Revealed by Proteomics in Mouse Kidney.

Biol Trace Elem Res 2021 May 15;199(5):1929-1940. Epub 2020 Aug 15.

Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.

Cadmium (Cd) is a highly toxic metal and kidney is its main target. However, the molecular effects and associated potential impacts of Cd-accumulated kidney have not been well investigated. In this study, mouse was used as a model to investigate the Cd-induced proteomic profile change in kidney, and a total of 34 differentially expressed proteins were detected by two-dimensional gel electrophoresis (2-DE) and further identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Through Gene Ontology analysis and KEGG pathway annotation, it showed that Cd-regulated kidney metabolism and promoted renal damage and cell migration. By validation of Western blotting and RT-qPCR, metastasis-related proteins LIM and SH3 domain protein 1 (LASP1) and phosphoenolpyruvate carboxykinase/cytosolic [GTP] (PEPCK1) were confirmed to be upregulated; Acyl-CoA synthetase medium-chain family member 3 (ACSM3) was downregulated. Furthermore, carcinoma development-related proteins initiation factor 4A (eIF4A) and pyridoxine-5'-phosphate oxidase (PNPO) were upregulated, and pyridoxal kinase (PK) was downregulated. The downregulation of Na(+)/H(+) exchange regulatory cofactor (NHERF3) might promote renal damage which associated with decrease of transferrin (TRF) in kidney. Taken together, our results revealed proteomic profile of Cd-induced nephrotoxicity and provided data for further insights into the mechanisms of Cd toxicity.
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http://dx.doi.org/10.1007/s12011-020-02312-7DOI Listing
May 2021

Edge detection algorithm of cancer image based on deep learning.

Bioengineered 2020 12;11(1):693-707

Department of Mechanical Engineering, Purdue University , Indianan, IN, USA.

For the existing medical image edge detection algorithm image reconstruction accuracy is not high, the fitness of optimization coefficient is low, resulting in the detection results of low information recall, poor smoothness and low detection accuracy, we proposes an edge detection algorithm of cancer image based on deep learning. Firstly, the three-dimensional surface structure reconstruction model of cancer image was constructed. Secondly, the edge contour feature extraction method was used to extract the fine-grained features of cancer cells in the cancer image. Finally, the multi-dimensional pixel feature distributed recombination model of cancer image was constructed, and the fine-grained feature segmentation method was adopted to realize regional fusion and information recombination, and the ultra-fine particle feature was extracted. The adaptive optimization of edge detection was realized by combining with deep learning algorithm. The adaptive optimization in the process of edge detection was realized by combining with the deep learning algorithm. The experimental results show that the three-dimensional reconstruction accuracy of the proposed algorithm is about 95%, the fitness of the optimization coefficient is high, the algorithm has a strong edge information detection ability, and the output result smoothness and the accuracy of edge feature detection are high, which can effectively realize the detection of cancer image edge.
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http://dx.doi.org/10.1080/21655979.2020.1778913DOI Listing
December 2020

HD-ZIP IV gene Roc8 regulates the size of bulliform cells and lignin content in rice.

Plant Biotechnol J 2020 12 8;18(12):2559-2572. Epub 2020 Jul 8.

Joint CAAS/IRRI Laboratory for Photosynthetic Enhancement, Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement, The Chinese Academy of Agricultural Sciences, Beijing, China.

The morphology of bulliform cells located on the upper epidermis of leaves is one of the most important cell structures affecting leaf shape. Although many mechanisms regulating the development of bulliform cells have been reported, the fine regulatory mechanisms governing this process have rarely been described. To identify novel components regulating rice leaf morphology, a mutant showing a constitutively rolling phenotype from the seedling stage to flowering, known as crm1-D, was selected for further analysis. Anatomical analyses in crm1-D were attributable to the size reduction of bulliform cells. The crm1-D was controlled by a single dominant nuclear gene. Map-based cloning revealed that Roc8, an HD zipper class IV family member, was responsible for the crm1-D phenotype. Notably, the 50-bp sequence in the 3'-untranslated region (3'-UTR) of the Roc8 gene represses Roc8 at the translational level. Moreover, the roc8 knockdown lines notably increased the size of bulliform cells. A series of assays revealed that Roc8 negatively regulates the size of bulliform cells. Unexpectedly, Roc8 was also observed to positively mediate lignin biosynthesis without incurring a production penalty. The above results show that Roc8 may have a practical application in cultivating materials with high photosynthetic efficiency and low lignin content.
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http://dx.doi.org/10.1111/pbi.13435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7680540PMC
December 2020

Human follicular fluid proteome reveals association between overweight status and oocyte maturation abnormality.

Clin Proteomics 2020 8;17:22. Epub 2020 Jun 8.

Central Laboratory, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, 264000 Shandong People's Republic of China.

Background: Human follicular fluid (HFF), which is composed by essential proteins required for the follicle development, provides an important microenvironment for oocyte maturation. Recently, overweight status has been considered as a detrimental impact factor on oocyte maturation, but whether HFF proteome could provide protein markers for assessing overweight-based oocyte maturation deficiency is still unknown.

Methods: To reveal the HFF-based molecular characteristics associated with abnormal oocyte maturation, an iTRAQ-based comparative proteomic analysis was performed to investigate different HFF protein expression profiles from normal weight women and overweight status women.

Results: Two hundred HFF proteins were quantified in our data, of which 43% have not been overlapped by two previous publications. Compared with the HFF proteins of normal weight women, 22 up-regulated HFF proteins and 21 down-regulated HFF proteins were found in the overweight status women. PANTHER database showed these altered HFF proteins participated in development, metabolism, immunity, and coagulation, and STRING database demonstrated their complicated interaction networks. The confidence of proteomic outcome was verified by Western blot analysis of WAP four-disulfide core domain protein 2 (WFDC2), lactotransferrin (LTF), prostate-specific antigen (KLK3), fibronectin (FN1), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Further, ELISA assay indicated WFDC2 might be a potentially useful candidate HFF marker for the diagnosis of oocyte maturation arrest caused by overweight status.

Conclusions: Our work provided a new complementary high-confidence HFF dataset involved in oocyte maturation, and these altered HFF proteins might have clinical relevance and diagnostic and prognostic value for abnormal oocyte maturation in overweight status women.
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http://dx.doi.org/10.1186/s12014-020-09286-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7282111PMC
June 2020

Significant association between rs28362491 polymorphism in NF-κB1 gene and coronary artery disease: a meta-analysis.

BMC Cardiovasc Disord 2020 06 8;20(1):278. Epub 2020 Jun 8.

Department of Cardiology, Hebei Province Hospital of Traditional Chinese Medicine, Zhongshan East street 389#,, Shijiazhuang, 050011, Changan District, China.

Background: The association of rs28362491 polymorphism in NF-κB1 gene and coronary artery disease (CAD) risk was reported in several studies with inconsistent outcomes. This study aimed to comprehensively collect and synthesize the existing evidence to appraise whether rs28362491 was correlated to CAD susceptibility.

Methods: Databases of Web of Science, EMBASE, PubMed, Wanfang, and CNKI were retrieved from inception to August 1, 2019 without any restriction on language. The strengths of association between rs28362491 polymorphism and CAD were presented as odds ratios (ORs) and 95% confidence intervals (CIs).

Results: Thirteen case-control studies with 17 individual cohorts containing 9378 cases and 10,738 controls were incorporated into this meta-analysis. The findings indicated that rs28362491 polymorphism was significantly correlated to CAD risk in five genetic models: D vs. I, OR = 1.16, 95%CI 1.11-1.21, P<0.01; DD vs. II, OR = 1.37, 95%CI 1.25-1.49, P<0.01; DI vs. II, OR = 1.11, 95%CI 1.05-1.18, P<0.01; DD + DI vs. II, OR = 1.17, 95%CI 1.11-1.24, P<0.01; DD vs. DI + II, OR = 1.29, 95%CI 1.15-1.43, P<0.01. After stratification by ethnicity and gender, significant association still existed between rs28362491 and CAD, especially in the dominant model.

Conclusions: The findings suggest that the mutant D allele in rs28362491 locus may increase the risk of CAD, and carriers of D allele appear to be more susceptible to CAD.
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http://dx.doi.org/10.1186/s12872-020-01568-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7282174PMC
June 2020

Long non-coding RNA FAM66C is associated with clinical progression and promotes cell proliferation by inhibiting proteasome pathway in prostate cancer.

Cell Biochem Funct 2020 Dec 19;38(8):1006-1016. Epub 2020 May 19.

Department of Urology, Affiliated Hospital of Jiangsu University, Zhenjiang, China.

Prostate cancer is the most prevalent malignancy in men, and the identification of novel oncogenes is clinically valuable for early screening, prevention and treatment. Recently, the studies have revealed that long non-coding RNAs (lncRNAs) play important roles in the development and progression of cancers including prostate cancer. The present study aims to identify a novel lncRNA that correlated with the survival time of prostate cancer patients and try to explore its biological functions in prostate cancer cells. After analysing the prostate carcinoma dataset of the Cancer Genome Atlas (TCGA), the lncRNA FAM66C was screened with its expression highly correlated with patient survival time, tumour stage and Gleason pattern. Real-time PCR showed that FAM66C highly expressed in prostate cancer cells, and knockdown FAM66C by siRNAs resulted in significant inhibition of cell growth. Furthermore, the results indicated that FAM66C promoted cell growth due to increasing cell proliferation but not decreasing cell apoptosis. In addition, FAM66C activated the epidermal growth factor receptor (EGFR)-extracellular signal-regulated kinase (ERK) signalling to promote cell proliferation. The result of Western Blotting and lysosomal acidity detection showed that knockdown FAM66C increased the protein ubiquitination and the lysosomal acidity. Moreover, inhibition of proteasome pathway could increase the activation of EGFR-ERK signalling and cell proliferation. Taken together, these results suggested that lncRNA FAM66C activate EGFR-ERK signalling to promote cell proliferation by inhibiting proteasome pathway in prostate cancer. SIGNIFICANCE OF THE STUDY: We demonstrated that lncRNA FAM66C was associated with clinical progression. In addition, highly expressed lncRNA FAM66C in prostate cancer cell lines promoted cell proliferation. Moreover, lncRNA FAM66C activate the epidermal growth factor receptor (EGFR)-extracellular signal-regulated kinase (ERK) signalling to promote cell proliferation by inhibiting proteasome pathway in prostate cancer. This study might provide lncRNA FAM66C as a potential therapeutic target gene of prostate cancer.
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http://dx.doi.org/10.1002/cbf.3531DOI Listing
December 2020