Publications by authors named "Yansong Ge"

13 Publications

  • Page 1 of 1

Dual Interface Protection for High Performance and Excellent Long-Term Stability of Organic Solar Cells.

ACS Appl Mater Interfaces 2021 Dec 29;13(48):57664-57672. Epub 2021 Nov 29.

School of Material Science and Engineering, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.

Stability is still the main barrier to the commercial application of organic solar cells (OSCs), although the maximal power conversion efficiency (PCE) value has exceeded 19%. The encapsulation technique is an effective and vital way to guarantee the long-term stabilities of OSCs, but it can only avoid the penetration of water and oxygen from the environment. Herein, we introduced a structure that provides dual interface protection by using commercially available and chemically stable polyvinylidene fluoride (PVDF) as the cathode interface protection layer working as the cathode interlayer (CIL) and poly(styrene-comethyl-methacrylate) (PS-r-PMMA) as the anode interface protection layer between the poly(3,4-ethylenedioxythiophene)/poly(styrene sulfonate) (PEDOT:PSS) and the active layer. With this structure, both the migration of impurities caused by degradation of the interfacial layer and the infiltration of oxygen and water in the air can be prevented. PVDF can effectively provide optimal electron transfer by improving the surface potential of active layers and lowering the work function of the Al electrode. PS-r-PMMA can improve the hydrophobicity of PEDOT:PSS and induce optimized phase separation, facilitating charge transfer. After storage in an air environment with a humidity of approximately 60% for 3600 h, the device based on the PM6:IT-4F blend film with dual interface protection showed a decrease in its PCE value from 13.43 to 10.90%, retaining 81.2% of its original PCE value, in contrast to the sharp decrease in the PCE value from 13.66 to 0.74% of the device without dual interface protection. The dual interface protection design could also be useful in the high-performance PM6:Y6 system, which shows a champion PCE of 15.39% and shows potential for the effective fabrication of stable OSCs in the future.
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http://dx.doi.org/10.1021/acsami.1c15792DOI Listing
December 2021

Protein kinase N1 promotes proliferation and invasion of liver cancer.

Exp Ther Med 2021 Jun 19;21(6):651. Epub 2021 Apr 19.

Department of Radiology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong 264100, P.R. China.

Protein kinase (PK) N1, also called PKC-related protein 1, participates in the proliferation, invasion and metastasis of various malignant tumors. However, the role of PKN1 in liver cancer remains to be elucidated. The present study investigated the expression of PKN1 using immunohistochemistry in surgical specimens from 36 patients and analyzed the correlation with VEGF, microvascular density (MVD), cell proliferation index (Ki67) and clinicopathological parameters. PKN1 was highly expressed in hepatocellular carcinoma (HCC) and was positively correlated with histological grading of HCC, Ki67 expression and MVD. PKN1 expression in moderately and poorly differentiated HCC was significantly higher compared with highly differentiated HCC. Expression of PKN1 was positively correlated with Ki67 and MVD, and Ki67 expression was positively correlated with MVD. The effects of PKN1 on proliferation, invasion and apoptosis of liver cancer cells were detected . Cell viability, migration and invasion were reduced and the apoptosis rate was significantly improved when PKN1 expression was silenced in liver cancer cells. Thus, PKN1 serves an important role in the development and progression of liver cancer. Inhibition of PKN1 activity may provide a promising therapeutic target for liver cancer.
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http://dx.doi.org/10.3892/etm.2021.10083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097187PMC
June 2021

Relative CT number of periappendiceal fat stranding may be an applicable index for estimating the severity of acute appendicitis.

Br J Radiol 2021 Apr 8;94(1120):20200437. Epub 2021 Mar 8.

Department of Radiology, Yantai Affiliated Hospital of Binzhou Medical University, No 717 Jinbu Road, Yantai, Shandong 264100, PR China.

Objectives: To investigate the feasibility of relative CT numbers to periappendiceal fat attenuation as an applicable index for estimating the severity of acute appendicitis.

Methods: In total, 308 consecutive surgery-confirmed acute appendicitis patients and 243 controls with available preoperative CT were analyzed retrospectively. The radiological parameters were appendix diameter, length, and wall thickness as concurrent appendicitis signs. CT numbers of periappendiceal fat, mesenteric fat, subcutaneous fat in the anterior and posterior abdominal wall, retroperitoneal fat, gluteal subcutaneous fat and psoas major muscle were measured, as well as the relative CT numbers of periappendiceal fat compared with other locations.

Results: There were 287 suppurative acute appendicitis (SAA) and 21 gangrenous or perforated acute appendicitis (GPAA) cases confirmed by pathology. The CT number of periappendiceal fat was significantly higher in patients than in controls (0.01) although there was a wide overlap (-72.33 HU-117.43 HU). Significant differences in relative CT numbers were observed between the groups in gluteal subcutaneous fat (R) and psoas major muscle (R) (<0.01). The AUCs of R and R showed high accuracy to discriminate acute appendicitis from controls (AUC = 0.803, 0.761; 0.854, 0.847) and GPAA from SAA (AUC = 0.905, 0.851).

Conclusions: Attenuation of periappendiceal fat on CT is related to the severity of appendicitis, and relative CT numbers (R and R) could be an applicable index for severity determination.

Advances In Knowledge: Periappendiceal fat infiltration is related to the severity of acute appendicitis (especially relative CT number). Other clinical and CT features also need to be considered in the evaluation of inflammation.
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http://dx.doi.org/10.1259/bjr.20200437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010530PMC
April 2021

Adipose-Derived Stem Cells Protect Ischemia-Reperfusion and Partial Hepatectomy by Attenuating Endoplasmic Reticulum Stress.

Front Cell Dev Biol 2020 20;8:177. Epub 2020 Mar 20.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

Ischemia-reperfusion (IR) is an inevitable complication of liver surgery. Recent studies indicate a critical role of endoplasmic reticulum stress (ERS) in hepatic IR. Mesenchymal stem cells (MSCs) have proven to be an effective tool for tissue regeneration and treatment of various diseases, including that of the liver. However, the mechanisms underlying the therapeutic effects of stem cells on hepatic IR injury (IRI) are still poorly understood, especially in the context of ERS. In this study, we established a porcine model of hepatic IRI and partial hepatectomy, and transplanted the animals with adipose-derived mesenchymal stem cells (ADSCs) isolated from miniature pigs. ADSCs not only alleviated the pathological changes in the liver parenchyma following IRI, but also protected the resident hepatocytes from damage. Mechanistically, the ADSCs significantly downregulated ERS-related proteins, including GRP78, p-eIF2α, ATF6 and XBP1s, as well as the proteins involved in ERS-induced apoptosis like p-JNK, ATF4 and CHOP. Taken together, ADSCs can alleviate hepatic IRI by inhibiting ERS and its downstream apoptotic pathways in the hepatocytes, indicating its therapeutic potential in liver diseases.
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http://dx.doi.org/10.3389/fcell.2020.00177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7098915PMC
March 2020

Polyolefin Elastomer as the Anode Interfacial Layer for Improved Mechanical and Air Stabilities in Nonfullerene Solar Cells.

ACS Appl Mater Interfaces 2020 Mar 24;12(9):10706-10716. Epub 2020 Feb 24.

Institute of Polymers and Energy Chemistry (IPEC), Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.

Despite the breakthroughs in power conversion efficiency (PCE) values of organic solar cells (OSCs), the other important issue concerns stability, which is urgently needed to be resolved for potential commercialization. A commercial and chemically stable polyolefin elastomer (POE) was incorporated into high-performance PBDB-T:ITIC, PM6:IT-4F, and PM6:Y6 nonfullerene systems to serve as the anode interfacial layer, affording remarkably improved mechanical and air stabilities when compared with those of the most studied MoO interfacial layer. The POE was found to selectively transport holes rather than electrons due to the upshifted surface contact potential of the active layer and the better ohmic contact between the active layer and the electrode. The POE serving as an encapsulating layer is supposed to suppress the penetration of water and oxygen in addition to the diffusion of Ag atoms into the active layer. After storing in an air environment with a humidity of approximately 70% for 150 days, the PCE of the device based on PM6:IT-4F with the POE anode interfacial layer decreased from 11.88 to 9.60%, retaining 80.8% of its original PCE value. The device using MoO as the anode interfacial layer showed a PCE value that was sharply reduced from 12.31 to 2.98% after storing for only 30 days. The POE could be potentially useful for flexible and large-scale device fabrication, accelerating the commercialization of OSCs.
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http://dx.doi.org/10.1021/acsami.9b18095DOI Listing
March 2020

Adipose-Derived Stem Cell Transplantation Attenuates Inflammation and Promotes Liver Regeneration after Ischemia-Reperfusion and Hemihepatectomy in Swine.

Stem Cells Int 2019 18;2019:2489584. Epub 2019 Nov 18.

Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.

Aim: To study the anti-inflammatory and liver regenerative effects of adipose-derived mesenchymal stem cells (ADSCs) on a porcine model of ischemia-reperfusion (IR) and hemihepatectomy.

Methods: Eighteen healthy Bama miniature pigs were randomly divided into the sham-operated (sham), untreated IR injury (IRI), and ADSC-transplanted (ADSC) groups. Hepatic IR was established by laparoscopic hemihepatectomy. ADSCs were transplanted directly into the liver parenchyma after the surgery. Hepatic inflammation and liver regeneration were evaluated by histopathological examination and assessment of relevant cytokines and other factors.

Results: ADSC transplantation successfully ameliorated the IRI-induced histopathological damage and the high levels of pro-inflammatory cytokines like IL-1, IL-6, and TNF-. In addition, the ADSCs enhanced the expression of the anti-inflammatory IL-10, regenerative factors including HGF, Cyclin D1, and proliferating cell nuclear antigen (PCNA), and angiogenic factors like VEGF, ANG-1, and ANG-2.

Conclusions: ADSCs attenuated the hepatic IRI-induced inflammatory response and promoted liver regeneration.
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http://dx.doi.org/10.1155/2019/2489584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6885808PMC
November 2019

Comparative study on protective effect of hydrogen rich saline and adipose-derived stem cells on hepatic ischemia-reperfusion and hepatectomy injury in swine.

Biomed Pharmacother 2019 Dec 24;120:109453. Epub 2019 Sep 24.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, PR China. Electronic address:

Aim: To compare and evaluate the hepatoprotective effect of liver parenchyma injection of ADSCs and portal vein injection of HRS in laparoscopic hepatic ischemia reperfusion combined with hepatectomy injury in miniature pigs.

Methods: Eighteen miniature pigs were randomly assigned to IRI group, HRS group and ADSCs group. HRS was injected through the portal vein 10 min before reperfusion, 1 d, 2 d, and 3 d after surgery. ADSCs were injected into liver parenchyma after hepatectomy. The serum and liver tissue samples were collected at different time points (preoperative, and postoperative at 1 d, 3 d and 7 d).

Results: Compared with the IRI group, both ADSCs and HRS groups can promote liver function recovery, reduce oxidative stress, reduce inflammation, and promote liver regeneration. Compared with HRS, ALT and TBIL in ADSCs group were significantly decreased at 3 d, and AST was significantly reduced at 1 d. The activities of SOD and GSH-Px in ADSCs group were significantly higher than that in HRS group, but the MDA level in HRS group was markedly lower than that in ADSCs group at 1 d. IL-1β was significantly lower in the ADSCs group than in the HRS group at 1 day after operation. The expressions of HGF and PCNA were significantly higher than that in the HRS group at 3 day after surgery.

Conclusion: Our study has demonstrated that HRS and ADSCs have significant hepatoprotective effects in miniature pigs after HIRI and hepatectomy injury. However, liver parenchyma injection of ADSCs is more beneficial to the recovery of liver function than portal vein injection of HRS.
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http://dx.doi.org/10.1016/j.biopha.2019.109453DOI Listing
December 2019

Corrigendum to "Effect of hydrogen-rich saline on apoptosis induced by hepatic ischemia reperfusion upon laparoscopic hepatectomy in miniature pigs" [Research in Veterinary Science, Volume 119, August 2018, Pages 285-291].

Res Vet Sci 2019 Oct 17;126:37. Epub 2019 Aug 17.

Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. Electronic address:

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http://dx.doi.org/10.1016/j.rvsc.2019.08.015DOI Listing
October 2019

Influence of Hydrogen-rich Saline on Hepatocyte Autophagy During Laparoscopic Liver Ischaemia-reperfusion Combined Resection Injury in Miniature Pigs.

J Vet Res 2018 Sep 10;62(3):395-403. Epub 2018 Dec 10.

Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.

Introduction: The purpose of this study was to investigate the protective effect of hydrogen-rich saline (HRS) against liver ischaemia-reperfusion combined resection injury.

Material And Methods: Eighteen miniature pigs were randomly divided into three groups: a sham operated group (sham group, laparoscopic liver ischaemia-reperfusion combined resection injury group (IRI group), and a hydrogen-rich saline intervention group (IRI + HRS group). Samples of hepatic tissue and serum were collected at the time of reperfusion and then 3 h, 1 d, and 3 d post reperfusion. Liver function, oxidative stress, autophagy-related mRNA genes, and protein expression were evaluated. Changes in cell and tissue ultrastructure were examined by transmission electron microscopy.

Results: Compared with the sham group, the level of autophagy of hepatocytes increased in the IRI and IRI + HRS groups, corresponding to high oxidative stress and severe liver function injury. Liver function, antioxidant content, autophagy levels, and liver injury were improved after intervention with HRS in the IRI + HRS group compared with the IRI group.

Conclusion: Intervention with hydrogen-rich saline could exert a protective effect against liver ischaemia-reperfusion combined resection injury through the reduction of oxidative stress and hepatocyte autophagy.
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http://dx.doi.org/10.2478/jvetres-2018-0056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295994PMC
September 2018

Adipose-derived stem cells alleviate liver apoptosis induced by ischemia-reperfusion and laparoscopic hepatectomy in swine.

Sci Rep 2018 11 15;8(1):16878. Epub 2018 Nov 15.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, P.R. China.

Hepatic ischemia-reperfusion (I/R) injury is inevitable during hepatectomy and may cause both postoperative morbidity and mortality. Regenerative medicine suggested adipose-derived stem cells (ADSCs) as an attractive tool for the treatment of liver diseases. In this study, we investigated the effect of ADSCs in an I/R model combined with laparoscopic hepatectomy in swine. Eighteen Bama miniature pigs were randomly divided into Sham, IRI, and ADSCs groups. ADSCs (1 × 10/kg) were injected through liver parenchyma immediately after hemihepatectomy. The apoptosis-related role of ADSCs was studied. The results showed that ADSCs transplantation reduced both pathological and ultrastructural changes and decreased the number of apoptotic-positive cells. In the ADSCs group, Fas, Fas ligand (FasL) protein, and mRNA were downregulated and the enzyme activities of Caspase3, Caspase8, and Caspase9 were significantly decreased. In addition, ADSC therapy significantly increased the ratio of Bcl-2/Bax protein and mRNA compared to the IRI group. In conclusion, ADSCs attenuated both I/R and hepatectomy-induced liver apoptosis in a porcine model, and offers a potential therapeutic option for hepatic I/R and hepatectomy.
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http://dx.doi.org/10.1038/s41598-018-34939-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237819PMC
November 2018

Adipose-derived stem cells reduce liver oxidative stress and autophagy induced by ischemia-reperfusion and hepatectomy injury in swine.

Life Sci 2018 Dec 29;214:62-69. Epub 2018 Oct 29.

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address:

Aims: Hepatic ischemia-reperfusion (I/R) injury is an unavoidable complication of liver surgery, often resulting in postoperative complications and liver dysfunction. Adipose-derived stem cells (ADSCs) have been recognized as a potential tool to be exploited in the field of regenerative medicine for the treatment of a variety of diseases. In the present study, the efficacy of ADSCs in a porcine I/R model of laparoscopic hepatectomy was evaluated.

Main Methods: Twelve Bama miniature pigs were randomly divided into IRI and ADSCs group. ADSCs were injected through the liver parenchyma following a partial laparoscopic hepatectomy. The roles of ADSCs on oxidative stress and autophagy were analyzed.

Key Findings: It was observed that serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (T-BIL) and lactate dehydrogenase (LDH) was significantly decreased in response to the ADSC treatment. The data were suggestive of the mechanism of action through which ADSCs exert their attenuating effects on oxidative stress by increasing the activity of superoxide dismutase (SOD), and by suppressing the generation of both myeloperoxidase (MPO) and malondialdehyde (MDA). Moreover, the expression of the critical markers of autophagy including Beclin1, ATG5, ATG12 and LC3II all decreased, whereas expression of P62 increased during phagophore expansion.

Significance: In conclusion, ADSCs attenuate hepatic I/R and hepatectomy-induced liver damage by reducing oxidative stress and inhibiting autophagy.
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http://dx.doi.org/10.1016/j.lfs.2018.10.054DOI Listing
December 2018

Effect of hydrogen-rich saline on apoptosis induced by hepatic ischemia reperfusion upon laparoscopic hepatectomy in miniature pigs.

Res Vet Sci 2018 Aug 23;119:285-291. Epub 2018 Jul 23.

Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. Electronic address:

Hepatic ischemia reperfusion injury (HIRI) occurs commonly in liver surgery and liver transplantation. Hydrogen, a safe and effective antioxidant, exerts a protective effect against liver injury. In this study, we investigated the role of hydrogen-rich saline (HRS) in apoptosis in a miniature pig model of laparoscopic HIRI upon hepatectomy. Bama miniature pigs were randomly assigned to sham, I/R and HRS groups. The pigs received 10 mL/kg HRS by portal venous injection 10 min before reperfusion and at 1 d, 2 d, and 3 d after surgery. The results showed that HRS treatment significantly decreased serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBIL) activity and TUNEL-positive cells. Upon HRS treatment, the expression of P53 and Bax mRNA and protein by RT-qPCR and Western blot was markedly decreased, whereas the expression of bcl-2 mRNA and protein was significantly increased. Moreover, Caspase-3 and Caspase-9 activities were significantly decreased upon treatment with HRS. In conclusion, the results indicate that HRS could alleviate liver injury and improve liver function via inhibiting apoptosis after laparoscopic HIRI and hepatectomy injury in miniature pigs.
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http://dx.doi.org/10.1016/j.rvsc.2018.07.005DOI Listing
August 2018

Hydrogen-rich saline protects against small-scale liver ischemia-reperfusion injury by inhibiting endoplasmic reticulum stress.

Life Sci 2018 Feb 15;194:7-14. Epub 2017 Dec 15.

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin 150030, PR China. Electronic address:

Aim: Our research investigated the role of Hydrogen-rich saline (HRS) on the Endoplasmic reticulum stress (ERS) pathway and the effect of HRS on tissue injury in small Bama pig model of hepatic ischemia-reperfusion combined with partial hepatectomy.

Main Methods: Eighteen healthy Bama miniature pigs were randomly divided equally into three groups: Sham, IRI, and HRS. Laparoscopic technique was employed to establish the model of hepatic ischemia-reperfusion combined with partial hepatectomy. HRS (10mL/kg) was injected into the portal vein 10min before perfusion. Histological examinations of the liver tissues were performed after HE staining. Additionally, transmission electron microscopy was performed to detect liver cell microstructure. Real-time PCR, Western blotting, and immunohistochemical staining were performed to analyze various ERS molecules including GRP78, p-eIF2α, XBP-1s, Full-length ATF6α, p-JNK, ATF4, and CHOP.

Key Findings: We observed that HRS visibly improved ischemia-reperfusion injury (IRI) by reducing various parameters of ERS stress as evidenced by down-regulation of the mRNA as well as protein levels of GRP78, p-eIF2α, XBP-1s, p-JNK, and CHOP, and reducing the cleavage of Full-length ATF6α.

Significance: Our study demonstrates that HRS protects the liver from IRI by inhibiting ERS.
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http://dx.doi.org/10.1016/j.lfs.2017.12.022DOI Listing
February 2018
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