Publications by authors named "Yanqun Liu"

90 Publications

Associations between the Gut Microbiome and Migraines in Children Aged 7-18 Years: An Analysis of the American Gut Project Cohort.

Pain Manag Nurs 2022 Jul 27. Epub 2022 Jul 27.

School of Nursing, Wuhan University, Wuhan, China.

Background: The gut microbiome seems to play a role in migraines through increasing intestinal epithelial permeability and pro-inflammatory processes. The associations between the gut microbiome and migraines are uncertain in children.

Aim: The purpose of this quantitative study was to examine the associations between the gut microbiome and migraines in children aged 7-18 years from the American Gut Project (AGP).

Method: A cohort of children aged 7-18 years from the AGP was analyzed. 16S rRNA V4 gene sequences for the gut microbiome, migraines, and demographics were obtained from the AGP Public Repository. After quality control of 16S rRNA gene sequences, α-diversity (Shannon, Faith's_PD, and evenness) and β-diversity metrics (Bray-Curtis and weighted-UniFrac distances), taxonomy, and abundance analyses were implemented using QIIME 2.

Results: In total, 381 children (341 without migraines; 40 with professional or self-diagnosed migraines) were analyzed with a mean age of 11.5 years. Compared with those without migraines, children with migraines showed lower estimates in Shannon and Faith's_PD (p < .01). Both Bray-Curtis and weighted-UniFrac distances displayed the gut microbial dissimilarities between these two groups (p = .001). Children with migraines had higher abundances in genus of phylum Bacteroidetes (Bacteroides, Parabacteroides, Odoribacter), Actinobacteria (Eggerthella, Varibaculum), Firmicutes (SMB53, Lachnospira, Dorea, Veillonella, Anaerotruncus, Butyricicoccus, Coprobacillus, Eubacterium), and Proteobacteria (Sutterella) than children without migraines.

Conclusions: Associations of the gut microbiome diversity and abundances with migraines in children indicated potential biological mechanisms of migraines. Future work needs to confirm our findings in children.
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http://dx.doi.org/10.1016/j.pmn.2022.06.002DOI Listing
July 2022

Psychometric evaluation of the pregnancy-related anxiety questionnaire-revised 2 for Chinese pregnant women.

Midwifery 2022 Jun 25;112:103411. Epub 2022 Jun 25.

Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, GA 30322, United States.

Introduction: Pregnancy related anxiety (PrA) is a unique type of anxiety during pregnancy. PrA can affect both maternal and infant health, as well as pregnancy outcomes. The purpose of this study was to culturally translate the Pregnancy-Related Anxiety Questionnaire-Revised 2 (PRAQ-R2) into Chinese and evaluate its psychometric properties in Chinese women during pregnancy irrespective of parity.

Methods: There were 411 Chinese pregnant women recruited from September 2020 to June 2021. The Cronbach's alpha, split-half reliability, and test-retest reliability were used to evaluate the reliability of PRAQ-R2 Chinese version. Validity was analyzed through content validity, convergent validity, discriminant validity and construct validity.

Results: The Chinese version of PRAQ-R2 showed good reliability and validity. The Cronbach's alpha coefficients of the total scale and subscales were all greater than 0.7. The median score (interquartile range, IQR) of PRAQ-R2 was 27 (32, 22). The correlation coefficient between item and hypothesis subscale were all greater than 0.40, indicating good convergence validity. Our findings revealed three dimensions of this scale by exploratory factor analysis and confirmed the original version of PRAQ-R2: worries about bearing a handicapped child, concern about own appearance, and fear of giving birth, with a total variance of 72.84% explained. The confirmatory factor analysis showed accepted model fit indexes.

Limitations: All pregnant women were recruited from one region in China, and the study lacked the longitudinal design. Future longitudinal multi-central studies are needed.

Conclusion: The Chinese version of PRAQ-R2 can be used as a promising tool to measure PrA in Chinese pregnant women without comorbidities and complications.
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http://dx.doi.org/10.1016/j.midw.2022.103411DOI Listing
June 2022

Psychometric evaluation of the body understanding measure pregnancy scale Chinese version for pregnant Chinese women.

Midwifery 2022 Jun 2;112:103394. Epub 2022 Jun 2.

Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, GA, 30322, USA.

Objectives: At present, there is limited research on pregnancy body image and no valid tool to measure body image in pregnant Chinese women. The purpose of this study was to translate the Body Understanding Measure Pregnancy Scale into Chinese to determine its reliability and validity in measuring body image in pregnant women.

Methods: The translation of the Body Understanding Measure Pregnancy Scale (BUMPs) was carried out with a standardized procedure. This study was conducted from July to December 2020 in the outpatient department of three tertiary hospitals in China. There were 1069 pregnant women completed the questionnaire and 1057 of those women were included in the analysis. Internal consistency, test-retest reliability, content validity, and construct validity of the translation version were examined.

Results: The final Chinese version of the BUMPs (BUMPs-C) had four dimensions with 16 items. Exploratory factor analysis obtained a three-factor solution, which explained 50.26% of the total variances. Confirmatory factor analysis showed that the model fit of the four-factor model was better than the three-factor model, and the four-factor model also reached a satisfactory model fit after modifying: Minimum Discrepancy was 2.82; Comparative Fit Index was 0.939, and Tucker-Lewis Index was 0.92; Root Mean Square Error of Approximation was 0.059. The content validity index of the scale was 1.0; the Cronbach's alpha (α) coefficient was 0.82 for the total scale, the McDonald's omega (ω) coefficient was 0.829; the test-retest reliability was 0.796.

Conclusions: The BUMPs-C showed good reliability and validity among pregnant Chinese women, which can be used as a simple and valid measurement tool to assess the feelings of pregnant Chinese women on body changes during pregnancy.
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http://dx.doi.org/10.1016/j.midw.2022.103394DOI Listing
June 2022

Case Report: A Chinese Family of Type A Insulin Resistance Syndrome With Diabetes Mellitus, With a Novel Heterozygous Missense Mutation of the Insulin Receptor Gene.

Front Endocrinol (Lausanne) 2022 12;13:895424. Epub 2022 May 12.

Department of Endocrinology, The People's Hospital of China Three Gorges University and The First People's Hospital of Yichang, Hubei, China.

Type A Insulin resistance syndrome (TAIRS) is an autosomal dominant or recessive genetic disorder caused by insulin dysfunction resulting from insulin receptor (INSR) gene mutation. The main features of TAIRS include hyperinsulinemia, abnormal glucose metabolism, and changes in acanthosis nigricans. We identified, in China, a TAIRS family with a novel heterozygous missense gene mutation type. One patient from the Chinese Han family exhibited signs and symptoms of TAIRS and was presented for evaluation. Whole-exome sequencing revealed a heterozygous mutation. Both the patient proband and his father were identified with insulin receptor exon 19c.3472C>T(p.Arg1158Trp), which resulted in a missense mutation that led to replace by a base in the amino acid codon. We found that the patient proband and his father exhibited high insulin and C-peptide release after glucose stimulation by insulin and C-peptide release tests. At the same time, we also ruled out the possibility of islet βcell tumor through relevant examinations. These findings indicate that the INSR gene mutation may cause pancreatic β cell functional impairment and contribute to the development of diabetes.
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http://dx.doi.org/10.3389/fendo.2022.895424DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134870PMC
May 2022

Impact of hepatitis B virus infection on maternal and infant outcomes of women with gestational diabetes mellitus: A three-year retrospective study.

J Diabetes Complications 2022 Jun 20;36(6):108201. Epub 2022 Apr 20.

School of Nursing, Wuhan University, No.115, Donghu Road, Wuchang District, Wuhan, 430071, Hubei Province, China. Electronic address:

Aims: To explore the impact of HBV infection on maternal and infant outcomes of GDM women.

Methods: We retrospectively identified 8126 women with GDM in China from July 2017 to June 2020, and divided them into GDM with HBV infection group (n = 483) and GDM with non-HBV infection group (n = 7643). Two sample t-test and Chi-square test were used to compare differences between groups. Logistic regression models were used to explore the association between HBV infection and maternal and infant outcomes.

Results: Placental abruption (PA), (2.3% vs. 1.0%, P = 0.008), placenta previa (4.3% vs. 2.8% p = 0.044), intrahepatic cholestasis of pregnancy (ICP), (6.4% vs. 3.0%, P < 0.001), cesarean section (52.0% vs. 46.0%, P = 0.011), fetal chromosomal abnormalities (1.2% vs. 0.4%, P = 0.021), and neonatal hyperglycemia (1.9% vs. 3.6%, P = 0.047) were more likely to occur in GDM with HBV infection group. After adjusting for the covariates, HBV infection was found to be associated with ICP (aOR, 2.35; 95% CI: [1.58-3.50]), PA (aOR, 2.34; 95% CI: 1.22-4.47), and fetal chromosomal abnormalities (aOR, 2.88; 95% CI: 1.18-7.03).

Conclusions: HBV infection was associated with part of maternal and infant outcomes in the GDM population.
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http://dx.doi.org/10.1016/j.jdiacomp.2022.108201DOI Listing
June 2022

Suppression of CCT3 inhibits melanoma cell proliferation by downregulating CDK1 expression.

J Cancer 2022 28;13(6):1958-1971. Epub 2022 Mar 28.

Department of Dermatology, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, China.

The eukaryotic chaperonin family is vital for cell survival. The dysregulation of chaperonin-containing TCP-1 subunit 3 (CCT3) is implicated in several types of malignant tumors' development. However, its functional role in melanoma remains unknown. Here we elucidate the functional contribution to CCT3 to melanoma progression. The results indicated that CCT3 highly expressed in melanoma tissues, and CCT3 overexpression is correlated with clinical stage in melanoma patients. Knockdown of CCT3 by shRNA in melanoma cells inhibited cell proliferation and cell cycle progression and induced cell apoptosis . , tumor growth in the nude mice was significantly inhibited after CCT3 silencing. Importantly, the gene array analysis showed that CCT3 depletions inhibited cyclins and cell cycle regulation signaling and further evaluation demonstrated that CDK1 expression was significantly decreased after CCT3 knockdown. Additionally, Functional rescues experiments also indicated that decreased cell proliferation due to CCT3 silencing was rescued by CDK1 overexpression. Overall, our findings suggest that CCT3 depletions prohibited melanoma progression by downregulating CDK1 expression and is a potential therapeutic target for melanoma.
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http://dx.doi.org/10.7150/jca.69497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8990421PMC
March 2022

A chromosome-scale genome assembly of the Mongolian oak (Quercus mongolica).

Mol Ecol Resour 2022 Aug 13;22(6):2396-2410. Epub 2022 Apr 13.

College of Horticulture, Shenyang Agricultural University, Shenyang, China.

Mongolian oak (Quercus mongolica Fisch.) is an ecologically and economically important white oak species native to and widespread in the temperate zone of East Asia. Here, we present a chromosome-scale reference genome assembly of Q. mongolica, a representative white oak species, by combining Illumina and PacBio data with Hi-C mapping technologies that is the first reference genome created for an Asian oak. Our results showed that the PacBio draft genome size was 809.84 Mb, with a BUSCO complete gene percentage of 92.71%. Hi-C scaffolding anchored 774.59 Mb contigs (95.65% of draft assembly) onto 12 pseudochromosomes. The contig N50 and scaffold N50 were 2.64 and 66.74 Mb, respectively. Of the 36,553 protein-coding genes predicted in the study, approximately 95% had functional annotations in public databases. A total of 435.34 Mb (53.75% of the genome) of repetitive sequences were predicted in the assembled genome. Genome evolution analysis showed that Q. mongolica is closely related to Q. robur from Europe, and they shared a common ancestor ~11.8 million years ago (Ma). Gene family evolution analysis of Q. mongolica revealed that the nucleotide-binding site (NBS)-encoding gene family related to disease resistance was significantly contracted, whereas the ECERIFERUM 1 (CER1) homologous genes related to cuticular wax biosynthesis was significantly expanded. This pioneering Asian oak genome resource represents an important supplement to the oak genomics community and will improve our understanding of Asian white oak biology and evolution.
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http://dx.doi.org/10.1111/1755-0998.13616DOI Listing
August 2022

Effects of thalassemia on pregnancy outcomes of women with gestational diabetes mellitus.

J Obstet Gynaecol Res 2022 May 24;48(5):1132-1140. Epub 2022 Feb 24.

Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, Georgia, USA.

Aim: To explore the effect of thalassemia on pregnancy outcomes of women with gestational diabetes mellitus (GDM).

Methods: This retrospective study reviewed the medical records of women with GDM delivered at the Chongqing Maternal and Child Health Hospital in China between July 2017 and December 2020. The live singleton pregnancies with α or β-thalassemia were identified as the thalassemia group, included α-thalassemia subgroup and β-thalassemia subgroup, whereas pregnant women without thalassemia were randomly selected as the non-thalassemia group according to a control-to-case ratio of 10:1 by computerized randomization. Logistic regression analyses were used to explore the potential association between thalassemia and pregnancy outcomes.

Results: A total of 223 pregnant women with GDM and thalassemia were analyzed, including women with α-thalassemia (n = 143) and β-thalassemia (n = 80). There were no significant differences in the incidence of adverse neonatal outcomes such as preterm birth and low-birth weight among groups. However, among pregnancy complications, significant differences were detected in the incidence of placenta increta, polyhydramnios, and postpartum anemia between the thalassemia group and the non-thalassemia group. Logistic regression results indicated that β-thalassemia increased the risk of polyhydramnios (odds ratio [OR] = 3.95, 95% confidence interval [CI]: 1.14-13.65, p = 0.030) and chorioamnionitis (OR = 3.61, 95%CI: 1.04-12.49, p = 0.043) compared with the non-thalassemia group.

Conclusion: In our study, thalassemia did not increase adverse neonatal outcomes, but β-thalassemia increased the risk of pregnancy complications, including polyhydramnios and chorioamnionitis.
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http://dx.doi.org/10.1111/jog.15206DOI Listing
May 2022

Relationship between the gut microbiota and temperament in children 1-2 years old in Chinese birth cohort.

J Psychiatr Res 2022 04 24;148:52-60. Epub 2022 Jan 24.

Emory University Nell Hodgson Woodruff School of Nursing, 1520 Clifton Road, Atlanta, GA, 30322, USA. Electronic address:

Background: Understanding the relationship between the gut microbiota and temperament can provide new insights for the regulation of behavioral intervention in children, which is still lacking research. This study aimed to examine the relationship between the gut microbiota and temperament in a cohort of children in 1 year and 2 years old.

Methods: This study included a total of 37 children with completed information, in which 51 samples at age 1 and 41 samples at age 2 were received respectively. We collected birth and demographic information. Parents reported their child's temperament characteristics using the Infant Behavior Questionnaire-revised (IBQ-R) and Early Childhood Behavior Questionnaire (ECBQ). Fecal samples were collected from each child at 1 and 2 years old and sequenced with MiSeq sequencer. Multiple linear regressions and linear mixed effect models were used to analyze the relationship between the temperament and their microbiota composition as well as the diversity and effect of gender or age on this relationship.

Results: At age of year 2, Faecalibacterium was negatively associated with high-intensity pleasure and surgency. Bifidobacterium was negatively correlated with Perceptual sensitivity. Results showed no difference about three domains between year 1 and year 2, while gut microbiota showed diversity difference and genera difference. There was no gender and age difference on the relationship between temperament and the gut microbiota.

Conclusions: Temperament was associated with the gut microbiota over time. The temperament remained stable and the relationship between the gut microbiota and temperament wasn't associated with age and gender.
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http://dx.doi.org/10.1016/j.jpsychires.2022.01.041DOI Listing
April 2022

Pain and Self-Management Status Among Chinese Patients With Cancer During the COVID-19 Pandemic.

Pain Manag Nurs 2022 02 4;23(1):26-30. Epub 2021 Oct 4.

School of Nursing, Wuhan University, Wuhan, China.

Background: To investigate the pain and self-management status of patients with cancer and the influencing factors of pain and self-management status during the COVID-19 pandemic.

Methods: A cross-sectional design was used. Eighty-one Chinese patients with cancer were recruited in December 2020. The Brief Pain Inventory, the Pain Management Inventory, and the Pain Self-efficacy Questionnaire were used to evaluate patients' pain and self-management status. Descriptive statistical analysis and multiple linear regression models were conducted for the research aims.

Results: Two thirds of the participants experienced moderate to severe pain. Cancer pain had moderate to severe interference on 90.12% of patients' lives. Self-management of pain in these participants was low. The most commonly used methods of pain management included adjusting activity intensity to avoid fatigue, using distraction techniques, and massaging the sore area. The most effective methods to manage pain included taking analgesics prescribed by doctor, taking over-the-counter analgesics, and massaging the sore area. Fifteen patients (18.5%) believed that the COVID-19 pandemic had an impact on pain management and 26 patients (32.1%) needed support. Pain education, pain interference on sleep, chemotherapy, and payment status were significantly associated with cancer patients 'pain self-management.

Conclusions: During the COVID-19 pandemic, patients with cancer had moderate to severe pain intensity with low levels of self-management and self-efficacy towards that pain.
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http://dx.doi.org/10.1016/j.pmn.2021.09.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8487793PMC
February 2022

Exploring the experience of nursing undergraduates in using gamification teaching mode based on the flow theory in nursing research: A qualitative study.

Nurse Educ Today 2021 Dec 29;107:105158. Epub 2021 Sep 29.

Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, GA 30322, USA. Electronic address:

Background: With the development of evidence-based nursing practice, nursing research courses are important in undergraduate programs. However, nursing research courses are highly theoretical, which is difficult for students to understand. Improving the teaching quality of nursing research courses is a challenge for educators.

Objectives: To understand the learning experience and the effect of gamification teaching on nursing undergraduates, and to provide new ideas for nurse educators.

Design: A qualitative research design using individual semi-structured interviews.

Settings: Gamification teaching was applied in an undergraduate nursing research course over one semester at a university in Central China.

Participants: Purposive sampling was adopted to select nine undergraduate nursing students who had recently completed gamification teaching in a nursing research course.

Methods: Data were collected through semi-structured interviews and analyzed using Colaizzi's phenomenological method.

Results: Three major themes and seven subthemes emerged from the interviewees' experience: (1) positive attitude (e.g., acceptance with pleasure and flow experience); (2) self-perceived competence improvement (e.g., creative thinking, collaboration, and knowledge internalization and application); and (3) challenges of gamification teaching (e.g., learning pressure and rationality of game design).

Conclusions: The gamification teaching mode based on the flow theory had a positive effect on the students' learning experience in the nursing research course. It is recommended to improve the game design model and to enhance its effectiveness for classrooms in the future.
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http://dx.doi.org/10.1016/j.nedt.2021.105158DOI Listing
December 2021

lipopolysaccharide affects oral epithelial connections via pyroptosis.

J Dent Sci 2021 Oct 26;16(4):1255-1263. Epub 2021 Jan 26.

Department of Dental Implantology, Hospital of Stomatology, Jilin University, Changchun, Jilin, China.

Background/purpose: Pyroptosis is a form of programmed cell death dependent on the activation of caspase-1. () is a major pathogenic bacterium in periodontitis and its lipopolysaccharide (LPS) can trigger inflammation. However, whether -LPS affects epithelial connections or triggers pyroptosis in the gingival epithelium is unknown.

Materials And Methods: Gingival samples from human donors were collected and the expression levels of E-cadherin, nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3), caspase-1/4/5, interleukin (IL)-18, and IL-1β were examined. -LPS was injected into rat gingival sulcus to establish gingivitis models, and the expression levels of E-cadherin, NLRP3, caspase-1/11, IL-18, and IL-1β were compared via immunohistochemistry. The mRNA levels of E-cadherin, caspase-1, IL-18, and IL-1β were evaluated in oral mucosa epithelial cells (OMECs) and rat gingival tissues.

Results: In the present study, NLRP3 ( < 0.01), caspase-1 ( < 0.01), caspase-4 (p = 0.044), and IL-18 (p = 0.036) expression was greater in the human inflammatory gingival samples, whereas E-cadherin (p = 0.045) had the opposite presentation. Gingivitis models were successfully established in rats with the injection of -LPS. NLRP3 ( = 0.015), caspase-1 ( < 0.01), caspase-11 ( < 0.01), and IL-18 (p = 0.041) were upregulated, whereas E-cadherin ( = 0.038) expression was decreased. Furthermore, E-cadherin mRNA was decreased while caspase-1, IL-18, and IL-1β mRNA levels were increased. The addition of a caspase-1 inhibitor reversed the expression changes.

Conclusion: -LPS may effectively destroy the epithelial connection by triggering pyroptosis.
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http://dx.doi.org/10.1016/j.jds.2021.01.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8403812PMC
October 2021

Effectiveness of the CenteringPregnancy program on maternal and birth outcomes: A systematic review and meta-analysis.

Int J Nurs Stud 2021 Aug 20;120:103981. Epub 2021 May 20.

Emory University Nell Hodgson Woodruff School of Nursing, 1520 Clifton Road, Atlanta, GA 30322, USA. Electronic address:

Background: The World Health Organization has emphasized the critical role of prenatal care in achieving the Millennium Development Goals to reduce child and maternal mortality. The CenteringPregnancy program is a widely recognized model of prenatal care. Several countries have attempted to implement the program in prenatal care practice; however, its effectiveness on maternal and birth outcomes has not been systematically evaluated and analyzed.

Objectives: To determine the effect of the CenteringPregnancy program on improving maternal and birth outcomes, including low birth weight, preterm birth, and postpartum depression.

Design: This study evaluated and analyzed randomized controlled trials by comparing the CenteringPregnancy program with o0bstetric led prenatal care. Maternal and birth outcomes of interest included low birthweight, preterm birth, and postpartum depressive symptoms.

Data Sources: Embase, PubMed, CINAHL, Web of Science, and The Cochrane Library were utilized in this systematic review. Additionally, a supplemental Google Scholar search was performed to capture all relevant articles.

Methods: All data were extracted independently by two trained researchers, who evaluated the quality of the study by examining the risk of bias. The biases of selection, allocation, measurement, reporting, and loss of follow-up were assessed using the Cochrane risk of bias for these included randomized controlled trials. A meta-analysis of eligible randomized controlled trials was conducted using Review Manager. Heterogeneity of studies was assessed using the I statistic.

Results: Out of 591 articles reviewed, seven randomized controlled trials were included in this study. Findings showed that the CenteringPregnancy program was not associated with lower rates of preterm birth (0.88 [0.71-1.07], p = 0.20, I = 0%), low birth weight (0.87 [0.68-1.12], p =0.29, I = 0%), or 12-month postpartum depressive symptoms (0.07 [-0.12-0.26], p =0.46, I = 69%). However, the CenteringPregnancy program was associated with reduced rates of 6-month postpartum depressive symptoms (0.49 [0.40-0.59], p < 0.01, I = 40%).

Conclusions: Existing evidence suggests that the CenteringPregnancy program and obstetric led care have similar effects on reducing the rates of preterm birth and low birth weight but different effects on postpartum depressive symptoms. More studies are needed to examine the effect of the CenteringPregnancy program on the improvement of postpartum depressive symptoms. PROSPERO Registration number: CRD42020171831. Tweetable abstract: We conducted a systematic review and meta-analysis of randomized controlled trials to determine the effects of the CenteringPregnancy program on improving maternal and birth outcomes, including low birth weight, preterm birth, and postpartum depressive symptoms. Seven randomized controlled trials were included in this study. The findings suggested that the CenteringPregnancy program and obstetric led care had similar effects in reducing the rates of preterm birth and low birth weight. More studies are needed to examine the effect of the CenteringPregnancy program on the improvement of postpartum depressive symptoms.
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http://dx.doi.org/10.1016/j.ijnurstu.2021.103981DOI Listing
August 2021

SP600125, a JNK-Specific Inhibitor, Regulates Auricular Cartilage Regeneration by Promoting Cell Proliferation and Inhibiting Extracellular Matrix Metabolism.

Front Cell Dev Biol 2021 16;9:630678. Epub 2021 Mar 16.

Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

construction is a major trend involved in cartilage regeneration and repair. Satisfactory cartilage regeneration depends on a suitable culture system. Current chondrogenic culture systems with a high content of transforming growth factor beta-1 effectively promote cartilaginous extracellular matrix (ECM) production but inhibit chondrocyte survival. As is known, inhibition of the c-Jun -terminal kinase (JNK) signaling pathway acts in blocking the progression of osteoarthritis by reducing chondrocyte apoptosis and cartilage destruction. However, whether inhibiting JNK signaling resists the inhibitory effect of current chondrogenic medium (CM) on cell survival and affects auricular cartilage regeneration (including cell proliferation, ECM synthesis, and degradation) has not been investigated. In order to address these issues and optimize the chondrogenic culture system, we generated a three-dimensional auricular cartilage regeneration model to investigate the effects of SP600125 (a JNK-specific inhibitor) on chondrocyte proliferation and ECM metabolism. SP600125 supplementation efficiently promoted cell proliferation at both cellular and tissue levels and canceled the negative effect of our chondrogenic culture system on cell survival. Moreover, it significantly inhibited ECM degradation by reducing the expressions of tumor necrosis factor-alpha, interleukin-1-beta, and matrix metalloproteinase 13. In addition, SP600125 inhibited ECM synthesis at both cellular and tissue levels, but this could be canceled and even reversed by adding chondrogenic factors; yet this enabled a sufficient number of chondrocytes to be retained at the same time. Thus, SP600125 had a positive effect on auricular cartilage regeneration in terms of cell proliferation and ECM degradation but a negative effect on ECM synthesis, which could be reversed by adding CM. Therefore, a combination of SP600125 and CM might help in optimizing current chondrogenic culture systems and achieve satisfactory cartilage regeneration by promoting cell proliferation, reducing ECM degradation, and enhancing ECM synthesis.
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http://dx.doi.org/10.3389/fcell.2021.630678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010669PMC
March 2021

Impacts of Maternal Diet and Alcohol Consumption during Pregnancy on Maternal and Infant Gut Microbiota.

Biomolecules 2021 03 1;11(3). Epub 2021 Mar 1.

Nell Hodgson Woodruff School of Nursing, Emory University, 1520 Clifton Road, Atlanta, GA 30322, USA.

(1) Background: Maternal diet and alcohol consumption can influence both maternal and infant's gut microbiota. These relationships are still not examined in the Chinese population. The purpose of this study was to explore the effect of alcohol consumption and maternal diet during pregnancy on maternal and infant's gut microbiota. (2) Methods: Twenty-nine mother-child dyads were enrolled in central China. Fecal samples of mothers during late pregnancy and of newborns within 48 h were collected. The V3-V4 regions of 16S rRNA sequences were analyzed. A self-administrated questionnaire about simple diet frequency in the past week was completed by mothers before childbirth. The demographic information was finished by mothers at 24 h after childbirth. (3) Results: Among these 29 mothers, 10 mothers reported alcohol consumption during pregnancy. The PCoA (β-diversity) showed significant difference in maternal gut microbiota between the alcohol consumption group vs. the non-alcohol consumption group (abund-Jaccard, r = 0.2, = 0.006). The same phenomenon was observed in newborns (unweighted-UniFrac full tree, r = 0.174, = 0.031). Maternal alcohol consumption frequency showed positive associations with maternal ( = 0.032) and ( = 0.019); maternal ( = 0.013) was negatively correlated with frequency of alcohol consumption. As for newborns, a positive relationship showed between ( = 0.035) and newborns with maternal alcohol consumption. The diet was not associated with both maternal and infant's gut microbiota. (4) Conclusions: Maternal alcohol consumption during pregnancy influenced the gut microbiota on both mothers and the newborns. Future research is needed to explore these relationships in a lager birth cohort. Understanding the long-term effect of alcohol consumption on maternal and newborns' gut microbiota is needed.
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http://dx.doi.org/10.3390/biom11030369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001387PMC
March 2021

A survey of postpartum depression and health care needs among Chinese postpartum women during the pandemic of COVID-19.

Arch Psychiatr Nurs 2021 04 17;35(2):172-177. Epub 2021 Feb 17.

West China School of Nursing/West China Hospital, Sichuan University, Chengdu, Sichuan, China. Electronic address:

Background: The COVID-19 pandemic seriously endangers the public's mental health, especially to pregnant and postpartum women. But little is known about postpartum depression and health care needs among Chinese postpartum women.

Aim: To investigate the status and risk factors of postpartum depression and health care needs among Chinese postpartum women during the COVID-19 pandemic.

Methods: In this cross-sectional study, 209 Chinese postpartum women were recruited from May to July 2020 by convenience sampling and assessed online with self-designed Maternal General Information Questionnaire, Edinburgh Postpartum Depression Scale (EPDS) and Chinese Version of the Perceived Stress Scale (CPSS). Descriptive statistics, chi-square test, independent samples t-test, one-way ANOVA, Pearson correlation and multiple linear regression were used for data analysis.

Results: With the EPDS cut-off value of 10, the incidence of postpartum depressive symptoms was 56.9%. Age, history of abortion and perceived stress were the influencing factors of postpartum depression (adjusted R = 0.432, F = 23.611, p < .001). The top three health care needs were infant rearing guidance (78.0%), maternal and infant protection guidance (60.3%) and dietary guidance (45.0%). The proportion of psychological rehabilitation guidance needs in the depressed group was significantly higher than that in the non-depressed group (34.5% vs. 20.0%, p < .05).

Conclusions: Maternal postpartum depression in China was at a high level during the COVID-19 pandemic. Women aged 25-34, with a history of abortion and high stress levels were at higher risk for postpartum depression. Timely psychological counselling, intervention and COVID-19-related health education are in great need for postpartum women.
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http://dx.doi.org/10.1016/j.apnu.2021.02.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886635PMC
April 2021

Near-Infrared Light-Triggered Thermosensitive Liposomes Modified with Membrane Peptides for the Local Chemo/Photothermal Therapy of Melanoma.

Onco Targets Ther 2021 25;14:1317-1329. Epub 2021 Feb 25.

Department of Clinical Medicine, Xuzhou Medical University, Xuzhou, 221004, People's Republic of China.

Purpose: A near-infrared (NIR)-triggered trans-activating transcriptional activator (TAT)-based targeted drug delivery system for the combined chemo/photothermal therapy of melanoma, namely, TAT-TSL-TMZ (temozolomide)/IR820, was developed for the first time.

Methods: TAT-TSL-TMZ/IR820 liposomes were synthesized via thin-film dispersion and sonication. IR820 and TMZ were encased in the inner layer and lipid bilayer of the liposomes, respectively.

Results: Dynamic light scattering results showed that the liposomes had an average hydrodynamic size of 166.9 nm and a zeta potential of -2.55 mV. The encapsulation rates of TMZ and IR820 were 35.4% and 28.6%, respectively. The heating curve obtained under near-infrared (NIR) laser irradiation showed that TAT-TSL-TMZ/IR820 liposomes had good photothermal conversion efficiency. The in vitro drug release curve revealed that NIR laser irradiation could accelerate drug release from TAT-TSL-TMZ/IR820 liposomes. The results of inverted fluorescence microscopy and flow cytometry proved that the uptake of TAT-TSL-TMZ/IR820 liposomes by human melanoma cells (MV3 cells) was concentration-dependent and that the liposomes modified with membrane peptides were more likely to be ingested by cells than unmodified liposomes. Confocal laser scanning microscopy indicated that TAT-TSL-TMZ/IR820 liposomes entered MV3 cells via endocytosis and was stored in lysosomes. In addition, TAT-TSL-TMZ/IR820 liposomes exposed to NIR laser showed 89.73% reduction in cell viability.

Conclusion: This study investigated the photothermal conversion, cell uptake, colocation and chemo/photothermal effect of TAT-TSL-TMZ/IR820 liposomes.
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http://dx.doi.org/10.2147/OTT.S287272DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920603PMC
February 2021

Keratinized mucosa augmentation guided by double xenogeneic collagen matrix membranes around implants in the posterior mandible: A case report.

Medicine (Baltimore) 2021 Jan;100(3):e23609

School and Hospital of Stomatology, Jilin University, Department of Dental Implantology, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun, Jilin Province, China.

Rationale: Traditional free gingival graft (FGG) technique is usually used for patients with insufficient peri-implant keratinized mucosa. However, this technique often requires a second surgical area which increases the pain as well as the risk of infection in patients. Xenogeneic collagen matrix (XCM) membrane technique can obtain good results for keratinized mucosa increment.

Patient Concerns: The patient was a 66-year-old healthy female with loss of left mandibular first molar and second molar (FDI #36, #37) for 5 years. Two implants were placed submucosally for 3 months with no interference, while a stage II surgery was needed.

Diagnosis: Probing depth measurements suggested that the mesial, medial, and distal widths of buccal keratinized mucosa within the edentulous area were 0.5, 0.5, and 1 mm, respectively, which were insufficient to maintain the health of peri-implant tissues.

Interventions: Keratinized mucosa augmentation guided by XCM membranes was performed to increase the inadequate buccal keratinized mucosa.

Outcomes: After 2 months of healing, the widths of mesial, medial, and distal buccal keratinized mucosa were 4, 3, and 3 mm, respectively, and the thickness of the augmented mucosa was 4 mm. Then a stage II surgery was followed. The patient was satisfied with the outcomes of keratinized mucosa augmentation.

Lessons: Keratinized mucosa augmentation guided by double XCM membrane technique can be applied to cases with keratinized mucosa width within 2 mm around implants.
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http://dx.doi.org/10.1097/MD.0000000000023609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837944PMC
January 2021

The experiences of nurses infected with COVID-19 in Wuhan, China: A qualitative study.

J Nurs Manag 2021 Jul 24;29(5):1180-1188. Epub 2021 Mar 24.

Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, GA, USA.

Aim: To understand about the experiences and feelings of the nurses infected with COVID-19 when caring for patients with COVID-19.

Background: With the sudden outbreak of coronavirus disease 2019 (COVID-19), nurses take care of patients with COVID-19 and have a very high risk of being infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) themselves.

Methods: This study adopted a qualitative design of hermeneutic phenomenology. A purposeful sampling was used, and all data were collected through in-depth semi-structured telephone interviews then analysed using interpretative phenomenological analysis.

Results: The interview data generated 4 topics and 14 secondary topics. Four major themes were identified from this study, including 'experiences of infection', 'health belief', 'social support' and 'the pursuit of self-worth'.

Conclusions: Nurses infected by COVID-19 experienced a physical and psychological shock. They had a strong sense of responsibility and willingness to take risks. Providing professional psychological counselling and physical rehabilitation services are crucial.

Implications For Nursing Management: This manuscript provides nursing managers an understanding of the personal experiences and needs of clinical nurses in their work, especially during COVID-19. It is helpful for nursing managers to explore the greater driving force of nurses and prepare nursing human resources for greater challenges.
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http://dx.doi.org/10.1111/jonm.13256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8013478PMC
July 2021

CD44-Targeting Oxygen Self-Sufficient Nanoparticles for Enhanced Photodynamic Therapy Against Malignant Melanoma.

Int J Nanomedicine 2020 22;15:10401-10416. Epub 2020 Dec 22.

Department of Dermatology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, People's Republic of China.

Objective: Nanotechnology-based photodynamic therapy (PDT) is a relatively new anti-tumor strategy. However, its efficacy is limited by the hypoxic state in the tumor microenvironment. In the present study, a poly(lactic-co-glycolic acid) (PLGA) nanoparticle that encapsulated both IR820 and catalase (CAT) was developed to enhance anti-tumor therapy.

Materials And Methods: HA-PLGA-CAT-IR820 nanoparticles (HCINPs) were fabricated via a double emulsion solvent evaporation method. Dynamic light scattering (DLS), transmission electron microscopy (TEM), laser scanning confocal microscopy, and an ultraviolet spectrophotometer were used to identify and characterize the nanoparticles. The stability of the nanoparticle was investigated by DLS via monitoring the sizes and polydispersity indexes (PDIs) in water, PBS, DMEM, and DMEM+10%FBS. Oxygen generation measurement was carried out via visualizing the oxygen bubbles with ultrasound imaging system and an optical microscope. Inverted fluorescence microscopy and flow cytometry were used to measure the uptake and targeting effect of the fluorescent-labeled nanoparticles. The live-dead method and tumor-bearing mouse models were applied to study the HCINP-induced enhanced PDT effect.

Results: The results showed that the HCINPs could selectively target melanoma cells with high expression of CD44, and generated oxygen by catalyzing HO, which increased the amount of singlet oxygen, ultimately inhibiting tumor growth significantly.

Conclusion: The present study presents a novel nanoplatform for melanoma treatment.
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http://dx.doi.org/10.2147/IJN.S283515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764953PMC
February 2021

Environmental Tobacco Smoke Exposure and Breastfeeding Duration Influence the Composition and Dynamics of Gut Microbiota in Young Children Aged 0-2 Years.

Biol Res Nurs 2021 07 3;23(3):382-393. Epub 2020 Dec 3.

Nell Hodgson Woodruff School of Nursing, 1371Emory University, Atlanta, GA, USA.

The colonization characteristics of infant gut microbiota are influenced by many factors at various stages, but few studies have explored the longitudinal effects of environmental tobacco smoke exposure and quantitative breastfeeding duration on young children' gut microbiota. We explored the effects of smoke exposure and breastfeeding duration on gut microbiota by following 37 maternal and children pairs in China for 2 years. We collected the demographic information, frequency of smoke exposure, breastfeeding duration, and fecal samples (mothers in the late pregnancy and infants at 6, 12, and 24 months), and analyzed the microbiota results using the V3-V4 gene sequence of 16S rRNA. The diversity of gut microbiota in children was the highest at 24 months and most similar to that in mothers. Breastfeeding duration was positively correlated with and negatively correlated with The α diversity of microbiota and the relative abundance of was higher in the non-smoke exposed group. The higher the smoke exposure, the higher the relative abundance of Prolonged breastfeeding and reduced smoke exposure are beneficial to the diversity and composition of gut microbiota in young children.
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http://dx.doi.org/10.1177/1099800420975129DOI Listing
July 2021

The experiences of pregnant women with gestational diabetes mellitus: a systematic review of qualitative evidence.

Rev Endocr Metab Disord 2021 12 12;22(4):777-787. Epub 2020 Nov 12.

Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, GA, 30322, USA.

Gestational diabetes mellitus (GDM) can have serious adverse effects on pregnant women and their fetuses. The purpose of this systematic review is to explore responses from women diagnosed with GDM and to describe their real-life experiences with self-management during pregnancy. The review provides insight into the physical, psychological, and social experiences of women with GDM; exploring these factors can help identify the challenges of glucose control and may provide targeted care and interventions to improve maternal and child health. Twelve databases were included in the initial article search, which was conducted on February 27, 2019. Two independent reviewers used the JBI Critical Appraisal Checklist for Qualitative Research for methodological validity to assess articles for study inclusion. The final synthesized findings were graded according to the ConQual approach for establishing confidence. Ten studies (totaling 223 pregnant women with GDM), originating from 6 different countries, were included in the review. Of the 223 women, 171 had one pregnancy affected by GDM and 52 had two or more pregnancies affected by GDM. Based on the findings from these studies, three main themes emerged, which were synthesized from 10 separate categories and 46 individual findings: beliefs about illness and health; life-changing experiences and healthy expectations; and difficulties and needs. Both positive and negative experiences of pregnant women with GDM can be understood as a process of "continuous struggle and change". Understanding the experiences of women diagnosed with GDM can provide health care professionals with more knowledge of how women experience GDM and develop feasible interventions to reduce the risk of developing type II diabetes.PROSPERO registration number CRD42019132065.
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http://dx.doi.org/10.1007/s11154-020-09610-4DOI Listing
December 2021

Human papillomavirus vaccination: coverage rate, knowledge, acceptance, and associated factors in college students in mainland China.

Hum Vaccin Immunother 2021 03 1;17(3):828-835. Epub 2020 Sep 1.

School of Health Sciences, Wuhan University, Wuhan, China.

In China, there are about 131,500 new cases of human papillomavirus (HPV) infection every year. However, studies focused on the related cognitions in the general college-going population, who belong to an at-risk age group and are of childbearing age, are relatively limited. Thus, this cross-sectional online survey study, conducted from December 2018 to March 2019, sought to investigate HPV vaccination rates, knowledge, acceptance, and associated factors in this population. Descriptive analysis and ordinal logistic regression analysis were conducted to analyze the factors associated with HPV vaccination intention. A total of 1,029 questionnaires were collected, of which 1,022 were valid (males: 267, females: 755). As per the results, only 3.1% of the sample had been vaccinated against HPV. The overall levels of knowledge about HPV and its vaccination were low. Male students' knowledge about HPV types, infection symptoms, vaccination cycles, and preventable diseases was significantly lower than that of female students. As for acceptance, only 36.9% of females and 24.8% of males indicated that they would choose to undergo HPV vaccination. Chinese college students' knowledge of HPV and its vaccination is limited. More than half of the sample was unsure about undergoing HPV vaccination, with concerns about safety and effectiveness serving as the main barriers. Measures such as strengthening health education, improving vaccination safety and effectiveness, and reducing vaccine prices should be taken to promote HPV vaccination among Chinese college students.
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http://dx.doi.org/10.1080/21645515.2020.1797368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993118PMC
March 2021

Effects of a health education program targeted to Chinese women adhering to their cultural practice of doing the month: A randomized controlled trial.

Midwifery 2020 Nov 18;90:102796. Epub 2020 Jul 18.

Emory University Nell Hodgson Woodruff School of Nursing, Atlanta, GA, USA.

Background: "Doing the month" is a prevalent Chinese postpartum custom which is believed to restore health after delivery. However, some traditional practices are potentially harmful for women's health.

Objectives: To examine the effect of an evidence-based health education program on Chinese postpartum women's adherence to traditional practices of doing the month and the effect of adherence to doing the month on maternal physiological and psychological health.

Methods: A randomized controlled trial was conducted. During December 2016-July 2017, we recruited postpartum women at a tertiary hospital. Women randomized to the intervention group received evidence-based health education within 1 week after returning home and received a second visit 1 month later. The control group received routine postpartum home visits. Adherence to doing the month was measured by the Adherence to Doing-the-Month Practices questionnaire (ADP). Maternal physical health was measured by the Chair Stand Test and Postpartum Symptom Checklist. Maternal psychological health was measured by the Edinburgh Postnatal Depression Scale (EPDS). Descriptive statistics, t-test, and chi-squared test were used to analyze the differences in scores and symptoms of the two groups.

Results: We recruited 124 eligible postpartum women and 108 of them (54 intervention group, 54 control group) completed this study. The ADP score of the intervention group was significantly lower than that of the control group (p < 0.001). The number of participants in the experimental group with poor appetite and indigestion was significantly lower than that of control group. No significant differences were found in numbers of symptoms and average EPDS scores between the 2 study groups (p > 0.05).

Conclusions: Evidence-based health education can reduce postpartum women's adherence to some traditional practices of doing the month and improve women's physical health.
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http://dx.doi.org/10.1016/j.midw.2020.102796DOI Listing
November 2020

Phenotypic redifferentiation of dedifferentiated microtia chondrocytes through a three-dimensional chondrogenic culture system.

Am J Transl Res 2020 15;12(6):2903-2915. Epub 2020 Jun 15.

Department of Facial Plastic and Reconstructive Surgery, Eye & ENT Hospital, Fudan University Shanghai, P. R. China.

Chondrocytes from microtia patients are a valuable cell source for the tissue-engineering of auricles. However, dedifferentiation of microtia chondrocytes remains an obstacle for clinical translation. Strategies, such as three-dimensional (3D) culture systems, and the use of chondrogenic growth factors, have successfully induced redifferentiation of dedifferentiated chondrocytes from healthy individuals. However, it remains unknown whether these strategies are similarly effective for microtia patient-derived chondrocytes, which may carry genomic defects. To address this issue, dedifferentiated microtia chondrocytes (DMCs) were cultured in a 3D chondrogenic culture system for 4-8 weeks to investigate their redifferentiated properties and to generate redifferentiated microtia chondrocytes (RMCs). To predict the degree and course of redifferentiation, RMCs at different time points were harvested and examined for cell morphology, cell proliferation, type II collagen expression at passaging, and chondrogenic capacity. We show that a 3D chondrogenic culture system can effectively induce DMCs to become redifferentiated, functional chondrocytes, enabling them to regenerate mature cartilage. Furthermore, RMCs achieved their full original function after culture in the chondrogenic culture system for 6-8 weeks. Interestingly, redifferentiation of microtia chondrocytes exhibited a time-dependent trend. Although the primary mechanism by which the 3D chondrogenic culture system regulated the transition of DMCs into RMCs remains unknown, the current study provides deeper insight into microtia chondrocytes and promotes clinical translation of tissue-engineered auricles.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344067PMC
June 2020

Mesenchymal Stem Cells Attenuated Blood-Brain Barrier Disruption via Downregulation of Aquaporin-4 Expression in EAE Mice.

Mol Neurobiol 2020 Sep 1;57(9):3891-3901. Epub 2020 Jul 1.

Department of Neurology, Shanghai Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China.

Blood-brain barrier disruption is one of the hallmarks of multiple sclerosis. Mesenchymal stem cells showed great potential for the multiple sclerosis therapy. However, the effect of mesenchymal stem cells on blood-brain barrier in multiple sclerosis remains unclear. Here, we investigated whether mesenchymal stem cells transplantation protected blood-brain barrier integrity and further explored possible underlying mechanisms. Adult female C57BL/6 mice were immunized with myelin oligodendrocyte glycoprotein peptide33-55 (MOG33-55) to induce experimental autoimmune encephalomyelitis (EAE). Mesenchymal stem cells (5 × 10) were transplanted via tail vein at disease onset. In the cell culture, we examined lipopolysaccharide-induced AQP4 upregulation in astrocytes. Results indicated that mesenchymal stem cells therapy improved neurobehavioral outcomes in EAE mice, reduced inflammatory cell infiltration, IgG protein leakage, and demyelination in spinal cord. Mesenchymal stem cells therapy also increased tight junction protein expression. In addition, mesenchymal stem cells downregulated AQP4 and A adenosine receptor (AAR) expression in EAE mice in spinal cord. We found that MSCs-conditioned medium (MCM) reduced the expression of inflammatory cytokines, AQP4 and AAR in lipopolysaccharide-activated astrocytes. BAY-60-6583 (a selective AAR agonist) reversed the MCM-induced AQP4 downregulation and increased p38 MAPK phosphorylation. Furthermore, the upregulation effects of AAR agonist were eliminated when treated with p38 MAPK inhibitor SB203580. Thus, we concluded that mesenchymal stem cells alleviated blood-brain barrier disruption by downregulating AQP4 in multiple sclerosis, possibly through inhibiting the AAR/p38 MAPK signaling pathway. Our work suggests that mesenchymal stem cells exert beneficial effect through maintaining blood-brain barrier integrity in EAE mice.
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http://dx.doi.org/10.1007/s12035-020-01998-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399688PMC
September 2020

Association between Gut Microbiota and Infant's Temperament in the First Year of Life in a Chinese Birth Cohort.

Microorganisms 2020 May 17;8(5). Epub 2020 May 17.

Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, GA 30322, USA.

Infant temperament characteristics play a critical role in children's developmental pathways and can predict adulthood psychopathology. The diversity and composition of the gut microbiota are associated with human temperament in both adults and young children. However, the relationship between the gut microbiota and temperament in 12-month-old infants is rarely studied; this developmental period is when temperament reaches a relatively stable stage. We used high-throughput sequencing methods to explore whether temperament characteristics were associated with gut microbiota diversity and composition. Infants' fecal samples were collected at 12 months of age for the gut microbiota analysis. Based on the primary caregivers' reports, infants' temperaments were measured using the Infant Behavior Questionnaire-revised (IBQ-R). This study included 51 infants, including 20 boys and 31 girls, with a mean age of 12.25 months. Results showed that soothability was positively correlated with maternal education level (β = 0.29, = 0.043, adjust = 0.025) and the abundance of genera (β = 0.62, = 0.004, adjust = 0.002). Conversely, cuddliness was negatively correlated with the abundance of genera. There was no significant difference in temperament based on gender. This study demonstrated that gut microbiota composition was associated with temperament in 12-month-old infants. These results point to the importance of gut microbiota balance. Future studies on the mechanisms behind the gut microbiota affecting temperament are warranted.
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http://dx.doi.org/10.3390/microorganisms8050753DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285300PMC
May 2020

Experiences of pregnant women with gestational diabetes mellitus: a systematic review of qualitative evidence protocol.

BMJ Open 2020 02 18;10(2):e034126. Epub 2020 Feb 18.

Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, Georgia, USA.

Introduction: The incidence of gestational diabetes mellitus (GDM) is increasing and an issue of global concern. GDM can cause severe adverse effects for pregnant women and their fetuses. This systematic review is proposed to explore women's experiences during the pregnancy with GDM. This review will provide insights into the physical, psychological and social adaptation experiences of women with GDM that can help to identify challenges of glycaemic control and provide targeted care and interventions to improve maternal and child health.

Methods And Analysis: The databases we will search include English databases (ie, PubMed, CINAHL, Embase, the Cochrane Library, Web of Science, Joanna Briggs Institute (JBI) Database of Systematic Reviews, PsycINFO, OpenGrey and Deep Blue) and Chinese databases (ie, China Biology Medicine disc, China National Knowledge Infrastructure, and VIP Database for Chinese Technical Periodicals). Published qualitative evidence of life changes or experiences of the women with GDM will be searched. There will be no limits on publication year. Two reviewers will independently use the JBI Critical Appraisal Checklist for Qualitative Research for methodological validity prior to inclusion in this review. Any disagreements regarding article evaluation will be resolved through discussion or with a third reviewer. Data will be extracted using the standardised data extraction tool from JBI System for the Unified Management, Assessment and Review of Information. Synthesis will include in-depth reading of the original text and the discovery of the results, and then summarising similar categories for more advanced synthesised findings. The final synthesised findings will be graded according to the ConQual approach for establishing confidence.

Ethics And Dissemination: This study does not require ethical approval as primary data will not be collected. Results of this systematic review will be submitted to peer-reviewed international journals for publication and be presented in relevant international conferences.

Prospero Registration Number: CRD42019132065.
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http://dx.doi.org/10.1136/bmjopen-2019-034126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044968PMC
February 2020

Evaluating Methane Adsorption Characteristics of Coal-Like Materials.

Materials (Basel) 2020 Feb 6;13(3). Epub 2020 Feb 6.

College of Safety Science and Engineering, Xi'an University of Science & Technology, Xi'an 710054, China.

In order to investigate the methane adsorption characteristics of coal seam materials in a "solid-gas" coupling physical simulation experiment, activated alumina, silica gel, the 3Å molecular sieve, 4Å molecular sieve and 5Å molecular sieve were selected as adsorption materials. According to the pore structure and adsorption characteristics, coal samples at the Aiweiergou #1890 working face were prepared as compared materials. The WY-98A methane adsorption coefficient measuring instrument was used to carry out this adsorption experiment under different temperatures, particle sizes and moisture contents. The results suggested that the adsorption principles of three kinds of molecular sieves under multiple factors do not fully fit a Langmuir adsorption model, and cannot be used as adsorption materials. The changing trend of the adsorption increment of activated alumina and silica gel are similar to that of coal samples, so they can be used as a coal-like materials. The methane adsorption coefficient value changing trends of activated alumina and silica gel appear to be the same as the Aiweiergou #1890 coal samples, but the results from silica gel are closer to that of coal samples. Thus, silica gel is preferred as the adsorption material. The result provides an experimental basis for the selection of methane-adsorbing materials and carrying out "solid-gas" coupling physical simulation experiments in a physically similar testing model.
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http://dx.doi.org/10.3390/ma13030751DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7040718PMC
February 2020

Endothelial progenitor cell transplantation alleviated ischemic brain injury via inhibiting C3/C3aR pathway in mice.

J Cereb Blood Flow Metab 2020 12 22;40(12):2374-2386. Epub 2019 Dec 22.

Department of Neurology, Ruijin Hospital, School of Medicine and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.

Endothelial progenitor cell transplantation is a potential therapeutic approach in brain ischemia. However, whether the therapeutic effect of endothelial progenitor cells is via affecting complement activation is unknown. We established a mouse focal ischemia model ( = 111) and transplanted endothelial progenitor cells into the peri-infarct region immediately after brain ischemia. Neurological outcomes and brain infarct/atrophy volume were examined after ischemia. Expression of C3, C3aR and pro-inflammatory factors were further examined to explore the role of endothelial progenitor cells in ischemic brain. We found that endothelial progenitor cells improved neurological outcomes and reduced brain infarct/atrophy volume after 1 to 14 days of ischemia compared to the control (<0.05). C3 and C3aR expression in the brain was up-regulated at 1 day up to 14 days (<0.05). Endothelial progenitor cells reduced astrocyte-derived C3 (<0.05) and C3aR expression (<0.05) after ischemia. Endothelial progenitor cells also reduced inflammatory response after ischemia (<0.05). Endothelial progenitor cell transplantation reduced astrocyte-derived C3 expression in the brain after ischemic stroke, together with decreased C3aR and inflammatory response contributing to neurological function recovery. Our results indicate that modulating complement C3/C3aR pathway is a novel therapeutic target for the ischemic stroke.
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http://dx.doi.org/10.1177/0271678X19892777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820683PMC
December 2020
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