Publications by authors named "Yanping Zhao"

114 Publications

Hypertension among Mongolian adults in China: A cross-sectional study of prevalence, awareness, treatment, control, and related factors: Hypertension among Mongolian adults in China.

J Clin Hypertens (Greenwich) 2021 Aug 16. Epub 2021 Aug 16.

Inner Mongolia Medical University, Hohhot, China.

The objectives of the study were to comprehend the prevalence of hypertension (HTN) and prehypertension (PHT), awareness, treatment, and control of HTN and its distribution in urban, agricultural, pastoral, and semi-agricultural/semi-pastoral areas, and to explore the related factors of HTN among Mongolian adults in China. From August 2018 to August 2020, a multi-stage stratified cluster random sampling method was conducted to investigate the prevalence of HTN among Mongolian adults aged ≥18 years living in China (n = 2558). Inclusion criteria for HTN were systolic blood pressure ≥ 140 mm Hg and/or diastolic blood pressure ≥ 90 mm Hg and/or had hypertensive history and/or taking antihypertensive drugs for HTN. The prevalence rates of HTN and PHT were 44.77% and 32.03%, respectively. The prevalence rates of PHT in urban, agricultural, pastoral, and semi-agricultural/semi-pastoral areas were 34.93%, 34.73%, 26.03%, and 33.44%, respectively, and the prevalence rates of HTN were 35.97%, 40.15%, 49.68%, and 48.07%, respectively. The awareness, treatment and control rates of HTN were 66.48%, 58.93%, and 16.48%, respectively. In this survey, the overweight, obesity, and central obesity rates were 34.30%, 30.67%, and 58.08%, respectively. Compared with Chinese adults ≥18 years, the prevalence rate of HTN among Mongolian adults in China aged ≥ 18 years was relatively high; the prevalence rate of PHT and HTN awareness, treatment, and control rates were similar. The prevalence of HTN and the rates of obesity and central obesity were higher in pastoral regions than in the other three types of regions, and the rate of overweight was highest in agricultural regions.
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http://dx.doi.org/10.1111/jch.14348DOI Listing
August 2021

Cell-free DNA as a diagnostic tool for human echinococcosis.

Trends Parasitol 2021 Jul 29. Epub 2021 Jul 29.

College of Life Science, University of Chinese Academy of Sciences, Shenzhen 518083, China; BGI PathoGenesis Pharmaceutical Technology, BGI-Shenzhen, Shenzhen 518000, China. Electronic address:

Echinococcosis is a major public health problem and an emerging/re-emerging disease. It was proposed that Echinococcus cell-free DNA (cfDNA) could be used in diagnosing human echinococcosis, though earlier attempts failed. Here, we update the research on detecting Echinococcus cfDNA in echinococcosis patients' samples and highlight future research directions.
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http://dx.doi.org/10.1016/j.pt.2021.07.006DOI Listing
July 2021

Analysis of MR Signs to Distinguish Between ARCO Stages 2 and 3A in Osteonecrosis of the Femoral Head.

J Magn Reson Imaging 2021 Jul 26. Epub 2021 Jul 26.

Department of Radiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Background: MRI is the most effective diagnostic tool of osteonecrosis of the femoral head (ONFH), especially for early diagnosis, but its detection of subchondral or cortical fractures is less accurate than CT. Therefore, it is difficult to accurately stage ONFH in the peri-collapse period by MRI.

Purpose: To improve the accuracy of MR for distinguishing between Association Research Circulation Osseous (ARCO) stages 2 and 3A in ONFH.

Study Type: Retrospective.

Subjects: One hundred and fifty five cases of ARCO stage 2/3A of ONFH underwent MR examinations, M/F = 72/83. CT was used as reference standard for collapse, which was decided by an orthopedist and a radiologist in consultation.

Field Strength/sequence: 3 T/axial and coronal T -weighted Turbo Spin Echo (T W TSE) sequence, axial T -weighted fat-saturated (T W FS) TSE sequence, and coronal proton density-weighted imaging (PDWI)-FS-Dixon fat/water image.

Assessment: Five potential MR signs (the maximum width of the necrotic-viable interface, bone marrow edema (BME), irregular articular surface of the femoral head, T heterogeneous high signal, and the absence of a necrotic-viable interface with the morphology of closed loop) were evaluated blindly by five radiologists independently and the total scores of different combinations of MR signs were calculated.

Statistical Tests: Mann-Whitney U test and Chi-square test were used to evaluate age, gender, and MR signs differences between the two groups. ROC curve was used to access the distinguishing value of MR signs. The consistency of the five radiologists was analyzed by intraclass correlation coefficient.

Results: The area under the curve of the combined MR signs 2 for distinguishing between ARCO stages 2 and 3A was the greatest (0.967), sensitivity and specificity were 100.00% and 88.71% respectively, and greater than 1 was the threshold.

Data Conclusion: Combined MR signs 2 has great values in distinguishing between ARCO stages 2 and 3A in ONFH, thus helping clinical therapy.

Level Of Evidence: 4 TECHNICAL EFFICACY: Stage 2.
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http://dx.doi.org/10.1002/jmri.27860DOI Listing
July 2021

Chemically Sulfated Polysaccharides from Agaricus blazei Murill: Synthesis, Characterization and Anti-HIV Activity.

Chem Biodivers 2021 Sep 9;18(9):e2100338. Epub 2021 Aug 9.

Beijing Key Laboratory of Environmental and Viral Oncology, Faculty of Environment and Life, Beijing University of Technology, Beijing, 100124, P. R. China.

AIDS, caused by HIV-1, is one of the most dangerous infectious diseases in the world. Therefore, it is necessary to develop new drugs with more potent bioactivities, less toxicity and higher tolerability for controlling the viral load, particularly by using the raw materials that are widely available. Agaricus blazei Murill (AbM), known in China as jisongrong, is of great importance as a food source and as a health-promoting supplement for immunomodulation. The polysaccharides of AbM exhibit various biological activities, such as regulating cellular immunity and providing anti-oxidative, anti-infective, and anti-inflammatory effects. At present, to our knowledge, no report has explored the chemically sulfated and anti-HIV-1 activity of AbM polysaccharides. Herein, the sulfated AbM polysaccharides with different sulfur contents were prepared by the chlorosulfonic acid-pyridine method. The characteristics of sulfated derivatives were established by the determination of the sulfur content, the relative molecular weight, and the Fourier-transform infrared spectroscopy. The anti-HIV activities of the sulfated AbM polysaccharides were evaluated by CCK-8 and the single-cycle pseudovirus infection (TZM-bl) assay. The sulfated AbM polysaccharides had strong antiviral properties, and the half-maximal inhibitory concentrations approached that of the positive control, azidothymidine. Sulfated modification of AbM polysaccharides can increase their anti-HIV pharmacological activity, which makes them promising alternative candidates as bioactive macromolecules for biomedical applications in HIV/AIDS.
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http://dx.doi.org/10.1002/cbdv.202100338DOI Listing
September 2021

[Up-regulated SUMO1 expression by inhibition of miRNA-548m as a potential cause of congenital orofacial cleft in a fetus].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Jul;38(7):647-651

Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.

Objective: To explore the genetic etiology for a fetus with congenital orofacial cleft.

Methods: Single nucleotide polymorphism microarray (SNP array) was carried out on skin tissues sampled from the fetus following induced abortion for the detection of copy number variation (CNVs). Pathogenicity of the candidate gene was validated through experiment.

Results: SNP array revealed that the fetus has carried a hemizygous 9.23Mb deletion at Xq21.31-q22.1(91 063 807-100 293 555), which was inherited from its mother. The region contained 13 OMIM genes and 1 ncRNA coding gene(MIR548M). Inhibiting of the expression of the MIR548M gene in oral epithelial celllines has resulted in up-regulation of the expression of SUMO1 gene which was known to involve in the pathogenesis of orofacial cleft.

Conclusion: Dosage insufficiency of the MIR548M gene may underlie the etiology of orofacial cleft in this fetus.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200307-00135DOI Listing
July 2021

4D-QSAR Molecular Modeling and Analysis of Flavonoid Derivatives as Acetylcholinesterase Inhibitors.

Biol Pharm Bull 2021 ;44(7):999-1006

Beijing Key Laboratory of Environmental and Viral Oncology, Faculty of Environment and Life, Beijing University of Technology.

Flavonoids are potential strikingly natural compounds with antioxidant activity and acetylcholinesterase (AChE) inhibitory activity for treating Alzheimer's disease (AD). In present study, in line with our interests in flavonoid derivatives as AChE inhibitors, a four-dimensional quantitative structure-activity relationship (4D-QSAR) molecular model was proposed. The data required to perform 4D-QSAR analysis includes 52 compounds reported in the literature, usually analogs, and their measured biological activities in a common assay. The model was generated by a complete set of 4D-QSAR program which was written by our group. The best model was found after trying multiple experiments. It had a good predictive ability with the cross-validation correlation coefficient Q = 0.77, the internal validation correlation coefficient R = 0.954, and the external validation correlation coefficient R = 0.715. The molecular docking analysis was also carried out to understand exceedingly the interactions between flavonoids and the AChE targets, which was in good agreement with the 4D-QSAR model. Based on the information provided by the 4D-QSAR model and molecular docking analysis, the idea for optimizing the structures of flavonoids as AChE inhibitors was put forward which maybe provide theoretical guidance for the research and development of new AChE inhibitors.
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http://dx.doi.org/10.1248/bpb.b21-00265DOI Listing
January 2021

A shining proposal for the detection of dissolved O in aqueous medium: Self-calibrated optical sensing via a covalent hybrid structure of carbon-dots&Ru.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Nov 25;261:120003. Epub 2021 May 25.

School of Materials Science & Engineering, Jiangsu University, Zhenjiang 212013, China.

O is a life-supporting gas and has been widely recognized as an important analyte in life science, medical care and environmental science. Optical sensing for gaseous oxygen has been widely reported owing to the simple, cost-effective and easy-to-go procedure. On the other hand, optical sensors for dissolved oxygen in aqueous media have been rarely reported, since most of them are incompatible with water, leading to poor sensitivity and linearity. In this effort, we tried the combination of Ru(II)-bpy complex and carbon dots (CDs) via covalent bonds, where bpy = bipyridine. A hybrid structure, named as [email protected], was constructed for the detection of dissolved oxygen, using Ru(II)-bpy as sensing probe and CDs as water-compatible supporting matrix. [email protected] was carefully characterized to confirm its hybrid structure. Detailed analysis suggested that its emission showed self-calibrated sensing signals for dissolved oxygen. A good linearity of 99.1% was realized. Its sensitivity (3.18) was higher than most literature values for dissolved oxygen detection. Its working equation was confirmed as a corrected Stern-Volmer equation (Lehrer mode). Good selectivity and signal stability were observed.
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http://dx.doi.org/10.1016/j.saa.2021.120003DOI Listing
November 2021

Molecular Cloning and Characterization of FAD6 Gene from Chia (Salvia hispanica L.).

Biochem Genet 2021 Apr 1. Epub 2021 Apr 1.

College of Agronomy and Biotechnology, Chongqing Rapeseed Engineering Research Center, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.

Plastidial Δ12 fatty acid desaturase (FAD6) is a key enzyme for linoleic acid (LA) and α-linolenic acid (ALA) biosynthesis. Chia (Salvia hispanica L.) is a revived omega-3 plant source that is richest in ALA level. In this study, based on the RACE method, one full-length cDNA sequence encoding FAD6, named ShFAD6, was isolated from chia. There exist three alternative transcription start sites and five alternative poly(A) tailing sites in ShFAD6. The 5'UTR of ShFAD6 contains a purine-stretch of 44 bp. ShFAD6 has an ORF of 1335 bp encoding a 444 aa protein of 51.33 kDa. ShFAD6 contains a conserved Delta12-FADS-like domain together with three strong trans-membrane helices and three histidine motifs. There also exists a chloroplast transmit peptide in ShFAD6 N-terminal. Phylogenetic analyses validated its identity of dicot FAD6 protein and suggested some critical evolutionary features of plant FAD6 genes. Heterologous yeast expression confirmed the catalytic activity of ShFAD6. The qRT-PCR assay showed that ShFAD6 is mainly expressed in leaves, stems, flowers, buds and early-stage seeds, and also responded to various stresses and hormone treatments. Under Sclerotinia infection, qRT-PCR and fluorescence imaging illustrated the possible correlation of ShFAD6 expression and photosynthesis. This study provides insight for further function study of ShFAD6 in oil quality improvement in staple oilseed crops as well as stress response and adaptation in plants.
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http://dx.doi.org/10.1007/s10528-021-10063-wDOI Listing
April 2021

Dexmedetomidine suppresses the development of abdominal aortic aneurysm by downregulating the mircoRNA‑21/PDCD 4 axis.

Int J Mol Med 2021 05 31;47(5). Epub 2021 Mar 31.

Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.

Abdominal aortic aneurysm (AAA) is a pathological state with permanent dilation, which indicates a fatal potential for aortic rupture. It has been reported that dexmedetomidine (Dex) and microRNA (miR)‑21 are involved in the progression of AAA. Thus, the present study aimed to investigate the joint effects of these factors on AAA treatment. For this purpose, rat models of AAA were established with enzyme perfusion and the rats were then injected with Dex. Alterations in the abdominal aorta in rats with AAA were recorded. miR‑21 expression in the rats with AAA was determined. Inflammatory factor expression was detected by western blot analysis. Subsequently, a dual‑luciferase reporter gene assay was performed to verify the targeting association between miR‑21 and programmed cell death protein 4 (PDCD4). Additionally, AAA‑related indices and inflammatory responses were examined by an injection of a combination of antagomiR (ant)‑miR‑21 and Dex or lentivirus‑PDCD4‑short hairpin RNA. It was found that Dex markedly alleviated the development of AAA and downregulated the expression of inflammatory factors and matrix metalloproteinase in rats with AAA. The high expression of miR‑21, which targets PDCD4, was observed in the rats with AAA. However, ant‑miR‑21 induced AAA development and inflammatory responses. Additionally, the inhibition of PDCD4 reduced AAA development and inflammatory responses. On the whole, the present study demonstrates that Dex inhibits AAA development by downregulating the miR‑21/PCDP4 axis. The findings of the present study may provide novel insight for the treatment of AAA. These findings may provide a reference for the future treatment of AAA and may provide theoretical guidance for the early prevention and development of AAA.
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http://dx.doi.org/10.3892/ijmm.2021.4923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8029612PMC
May 2021

Variants and the Interaction with Smoking Confer the Genetic Susceptibility to Ischemic Stroke.

Int J Med Sci 2021 23;18(8):1840-1847. Epub 2021 Feb 23.

Department of Epidemiology, School of Public Health, Nanjing Medical University, Nanjing 211166, China.

High temperature requirement protein A1 (1) was identified as the causative gene of autosomal recessive arteriopathy and associated with lacunar ischemic stroke (IS) in European. This study aimed at evaluating the association of with IS and four tagging single-nucleotide polymorphisms (SNPs) were genotyped in a cohort of 4,098 Chinese. The mRNA level of in 72 IS cases and 72 hypertension controls were measured and compared. In whole population, SNP rs2268350 (C>T) was significantly associated with IS incidence (=0.034). Stratification analysis observed significant association of rs2268350 in male, smoking and drinking populations, rs2672587 (C>G) in smoking and nonsmoking populations and rs3793917 (C>G) in smoking, nonsmoking and nondrinking populations with stroke respectively (<0.05). The additive interaction and multiplicative interaction between rs2268350 and smoking were both of significant (P<0.05) after adjustment for the covariates. There was a cumulated risk of IS among genotypes of rs3793917 (=0.009) and rs2672587 (=0.047) in smoking population. The mRNA level of in non-smokers with rs2268350 CC was significantly higher than smokers with rs2268350 CT/TT (P=0.046) in IS cases. Our findings support that confers the genetic susceptibility to IS and smoking might modify the genetic effect of on IS by suppressing mRNA expression.
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http://dx.doi.org/10.7150/ijms.45856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7976583PMC
February 2021

The variants at FLNA and FLNB contribute to the susceptibility of hypertension and stroke with differentially expressed mRNA.

Pharmacogenomics J 2021 Aug 1;21(4):458-466. Epub 2021 Mar 1.

Department of Epidemiology, School of Public Health, Nanjing Medical University, Nanjing, China.

Background: Filamin A and filamin B were involved in vascular development and remodeling. Herein, it is important to explore the associations of FLNA and FLNB variants with hypertension and stroke.

Methods: The associations of two single-nucleotide polymorphisms (SNPs) at FLNA and five SNPs at FLNB with hypertension and stroke were examined in two case-control studies and a cohort study in Chinese Han population. Risks were estimated as odds ratio (OR) and hazard ratio (HR) by Logistic and Cox regression analysis respectively. In addition, filamin B, FLNA and FLNB mRNA expression were measured.

Results: In the case-control study of hypertension, FLNA rs2070816 (CT + TT vs. CC) and rs2070829 (CG + GG vs. CC) were significantly associated with hypertension in <55 years group (OR = 1.338, P = 0.018; OR = 1.615, P = 0.005) and FLNB rs839240 (AG + GG vs. AA) was significantly associated with hypertension in females (OR = 0.828, P = 0.041) and nonsmokers (OR = 0.829, P = 0.020). In the follow-up study, rs2070829 GG genotype carriers presented a higher risk of hypertension than CC/CG in males (HR = 1.737, P = 0.014) and smokers (HR = 1.949, P = 0.012). In the case-control study of stroke, FLNB rs1131356 variation was significantly associated with ischemic stroke (IS) and intracerebral hemorrhage (ICH), ORs of additive model were 1.342 and 1.451, with P values of 0.001 and 0.007. The FLNA transcript 2, FLNB transcript 3, transcript 4 mRNA, and filamin B expression levels were significantly different between IS cases and hypertension controls and among the genotypes of rs839240 in hypertensive individuals (P < 0.05).

Conclusions: Our findings support the genetic contribution of FLNA and FLNB to hypertension, and stroke with differentially mRNA expression.
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http://dx.doi.org/10.1038/s41397-021-00222-yDOI Listing
August 2021

Contamination, distribution, and risk assessment of antibiotics in the urban surface water of the Pearl River in Guangzhou, South China.

Environ Monit Assess 2021 Jan 28;193(2):98. Epub 2021 Jan 28.

Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, Guangdong Provincial Engineering Research Center for Online Monitoring of Water Pollution, Institute of Analysis, Guangdong Academy of Sciences (China National Analytical Center, Guangzhou), Guangzhou, China.

To assess the impact of antibiotic pollution to the ecosystem in urban water, the occurrence, seasonal, and spatial distributions, potential sources, and ecological risks of 18 targeted antibiotics in urban river, Pearl River located in Guangzhou city, were investigated. Surface water samples were sampled from 24 sites in Guangzhou center of Pearl River during dry and wet seasons. The results indicated that the concentrations of antibiotic residues were at the nanogram per liter level, except sulfamethazine (SMD) (μg/L). Sulfonamides (SAs) were the dominant antibiotics, contributing 60.4-65.0% to the total antibiotics. The concentrations of SAs, fluoroquinolones (QUs), macrolides (MLs), tetracyclines (TCs), and lincosamides (LCs) were higher in dry season than those in wet season at most sampling sites, which possibly resulted from the dilution effect of heavy rainfall. The concentrations of the antibiotic residues in Guangzhou were comparable or higher than other urban rivers. The calculation on risk quotients indicated that erythromycin-HO (ETM-HO) and tetracycline (TC) were of high risks. The source identification by the Pearson correlation analysis and principal component analysis-multiple linear regression (PCA-MLR) method suggested that municipal wastewater treatment plants were primary sources of antibiotics. These results would provide important information for the environmental protect.
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http://dx.doi.org/10.1007/s10661-021-08887-5DOI Listing
January 2021

Seasonal iron‑sulfur interactions and the stimulated phosphorus mobilization in freshwater lake sediments.

Sci Total Environ 2021 May 25;768:144336. Epub 2020 Dec 25.

School of Environment, Nanjing Normal University, Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Jiangsu Key Laboratory of Environmental Change and Ecological Construction, Nanjing 210,023, China.. Electronic address:

Sulfur reduction in freshwater ecosystems has previously been considered as negligible because of often very low sulfate concentrations and generally low sulfate reducing capacity in freshwater sediments. In this study, seasonal variations on three types of sediments from central lake, dredged and algae accumulated areas in a eutrophic lake in China, Lake Taihu, were investigated. The high temperature in summer and the accumulation of algae are conducive to the reduction processes in freshwater lake sediments. Iron reduction was observed as the major anaerobic process in all types of sediments, while sulfate reduction was weak in central and dredged lake areas. However, strong sulfate reduction with increase of sulfate reducing bacteria and sulfides generation (119.5 ± 0.2 μmol L) was found in surface sediments in algae accumulated areas. Based on the results of Fe reduction rate and the quantity of Fe reducing bacteria, extensive sulfate reduction in algae accumulated sediments inhibited the microbial Fe reduction, and the ΣS-mediated chemical Fe reduction (SCIR) dominated instead. Iron was principally stored in the sediments as Fe sulfide compounds, which weakened the rebinding of phosphorus and stimulated phosphorus mobilization. Therefore, attention should be paid to the alteration of Fe cycling and phosphorus mobility caused by the SCIR in algae accumulated sediments and the consequent effects on the eutrophication of freshwater lakes.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144336DOI Listing
May 2021

Characterizing dynamic changes of plasma cell-free Echinococcus granulosus DNA before and after cystic echinococcosis treatment initiation.

Genomics 2021 Mar 28;113(2):576-582. Epub 2020 Dec 28.

Second People's Hospital of Tibet Autonomous Region, Lhasa 850000, China. Electronic address:

Over one million people are living with cystic echinococcosis (CE) and alveolar echinococcosis (AE). For CE, long-term albendazole treatment is often needed, which requires regular follow-up. Follow-up is mainly through imaging which is insensitive to subtle changes and subjective to experience. We investigated the changes of Echinococcus granulosus (Eg) cell-free DNA (cfDNA) in plasma of CE patients before and after albendazole treatment to evaluate its potential as an objective marker for treatment follow-up. Plasma samples of nine CE patients were collected before and after treatment. We identified Eg cfDNA from every sample through high-throughput sequencing. Eg cfDNA concentration and fragment length increased significantly after the treatment period. Ultrasound examination before and after the treatment initiation reflected the drug effects to a certain extent, as the cyst size of four patients reduced. Our findings indicated that Eg cfDNA from plasma could be a potential marker in the monitoring of CE treatment.
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http://dx.doi.org/10.1016/j.ygeno.2020.12.035DOI Listing
March 2021

Metabolomic profiling reveals the intestinal toxicity of different length of microplastic fibers on zebrafish (Danio rerio).

J Hazard Mater 2021 02 13;403:123663. Epub 2020 Aug 13.

School of Environment, Nanjing Normal University, Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Jiangsu Key Laboratory of Environmental Change and Ecological Construction, Nanjing, 210023, China.

To explore the intestinal toxicity of microplastic fibers, zebrafish larvae and adults were exposed to different length of microplastic fibers (50 ± 26 μm and 200 ± 90 μm). After exposure, microplastic fibers were observed in the gut of zebrafish even at the early life stage, causing length-dependent intestinal damage and toxicities manifested by histopathological changes and biomarker responses. Long microplastic fibers induced more serious effects. They significantly decreased the food intake of zebrafish by 54 %-67 % compared with short microplastic fibers. Metabolomics was conducted to further reveal the metabolic alterations induced by microplastic fibers in zebrafish. A total of 124 and 123 metabolites were significantly changed by short and long microplastic fibers. At the meanwhile, 41 significantly changed metabolites were shared between short and long fibers treatment groups and were further investigated to reveal the influence of fiber length on the toxicity. The results demonstrate that microplastic fibers can up-regulate glycerophospholipids metabolism which exacerbates oxidative damage and inflammation and down-regulate fatty acyls metabolism related to nutritional deficiency. These novel findings enhance our understanding of the intestinal toxicity of microplastic fibers and demonstrate that metabolomics is powerful to unravel the underlying mechanisms of microplastics (MPs) toxicity.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123663DOI Listing
February 2021

Dynamic sulfur-iron cycle promoted phosphorus mobilization in sediments driven by the algae decomposition.

Ecotoxicology 2021 Oct 2;30(8):1662-1671. Epub 2020 Dec 2.

School of Environment, Nanjing Normal University, Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Jiangsu Key Laboratory of Environmental Change and Ecological Construction, Nanjing, 210023, China.

Direct evidence of the algae bloom in eutrophic freshwater lakes on sulfur cycle and the subsequent iron oxide reduction and the iron oxides-bound phosphate (Fe-P) release in sediments is lacking. In this study, microcosms experiment was carried out to investigate the dynamic variations of S, Fe and P species in the water column and sediments as well as the sulfate reducing bacteria (SRB) abundance variation in the sediments during algae decomposition. The sulfate reduction was stimulated by the algae decomposition, which resulted in dramatic sulfate decline, sulfide increase and SRB growth. In addition, large amounts of acid volatile sulfide (AVS), pyrite sulfur (Pyrite-S) and elemental sulfur (S) accumulated in the sediment. In particular, the contents of sedimentary Fe(II) and Pyrite-S in surface sediments continuously accumulated until the end of the experiment. Moreover, the terminal Fe-P content reduced by 35.4% compared with the initial concentration at high algae density group. These results suggested the irreversible reduction of iron oxides and revealed iron chemical reduction mediated by sulfide during algae decomposition. In addition, the connection of sulfur-iron cycle and the significant promotion of Fe-P mobilization in sediments was established, which should be paid more attention in the eutrophic freshwater ecosystems.
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http://dx.doi.org/10.1007/s10646-020-02316-yDOI Listing
October 2021

Genetic signatures for lineage/sublineage classification of HPV16, 18, 52 and 58 variants.

Virology 2021 Jan 16;553:62-69. Epub 2020 Nov 16.

BGI-Shenzhen, Shenzhen, 518083, China; Shenzhen Key Laboratory of Unknown Pathogen Identification, BGI-Shenzhen, Shenzhen, 518083, China; School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China. Electronic address:

Increasing evidences indicate that high-risk HPV variants are heterogeneous in carcinogenicity and ethnic dispersion. In this work, we identified genetic signatures for convenient determination of lineage/sublineage of HPV16, 18, 52 and 58 variants. Using publicly available genomes, we found that E2 of HPV16, L2 of HPV18, L1 and LCR of HPV52, and L2, LCR and E1 of HPV58 contain the proper genetic signature for lineage/sublineage classification. Sets of hierarchical signature nucleotide positions were further confirmed for high accuracy (>95%) by classifying HPV genomes obtained from Chinese females, which included 117 HPV16 variants, 48 HPV18 variants, 117 HPV52 variants and 89 HPV58 variants. The circulation of HPV variants posing higher cancer risk in Eastern China, such as HPV16 A4 and HPV58 A3, calls for continuous surveillance in this region. The marker genes and signature nucleotide positions may facilitate cost-effective diagnostic detections of HPV variants in clinical settings.
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http://dx.doi.org/10.1016/j.virol.2020.11.003DOI Listing
January 2021

Role of Chain Extension in the Ability of Peptide Oligomers to Damage the Lipid Membrane Studied by the l- to d-Amino Acid Substitutions of hIAPP.

J Phys Chem B 2020 11 3;124(45):10147-10156. Epub 2020 Nov 3.

State Key Laboratory of Supramolecular Structure and Materials, Jilin University, 2699 Qianjin Avenue, Changchun, 130012, P. R. China.

Exploration of the relation between the structural feature of oligomers and the ability of oligomers to damage the membrane has been an important subject in the study of the cytotoxic mechanism of amyloid proteins. In this work, we selected the hIAPP fragment as a model peptide and modified it by an alternating substitution of a d-amino acid for an l-amino acid in the hydrophilic N-terminal region, the hydrophobic C-terminal region, and the entire sequence. We prepared the oligomers using these peptides and investigated the effects of chain extension in different regions of the peptide on the ability of the oligomers to damage the membrane composed of POPC/POPG 4:1. We examined the morphology, structure, surface hydrophobicity, and packing compactness of the oligomers and monitored the changes in the structure and aggregation of the peptides upon interaction with the membrane. We found that the surface hydrophobicity and the disruptive ability of the oligomers are increased by an alternating l- and d-amino acid arrangement in the hydrophobic region of the peptide, while the packing compactness of the oligomers is increased and the disruptive ability of the oligomers decreased by an alternating l- and d-amino acid arrangement only in the hydrophilic region. The extension of the hydrophobic chain plays a significant role in the disruptive ability of the oligomers. Our results suggest that a positive relation between the surface hydrophobicity and the disruptive ability could be established only for the oligomers in which the peptide chains are flexible and loosely packed.
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http://dx.doi.org/10.1021/acs.jpcb.0c07656DOI Listing
November 2020

Experimental Study on Whole Wind Power Structure with Innovative Open-Ended Pile Foundation under Long-Term Horizontal Loading.

Sensors (Basel) 2020 Sep 18;20(18). Epub 2020 Sep 18.

Shandong Bureau of China Metallurgical Geology Bureau, Jinan 250014, China.

The offshore wind energy (OWE) pile foundation is mainly a large diameter open-ended single pile in shallow water, which has to bear long-term horizontal cyclic loads such as wind and waves during OWE project lifetime. Under the complex cyclic loads, the stress and displacement fields of the pile-soil system change continuously, which affects the dynamic characteristics of the pile foundation. Within the service life of the pile foundation, the pile-soil system has irreversible cumulative deformation, which further causes damage to the whole structure. Therefore, it is important to examine the overall dynamic characteristics of wind power foundation under high cycle. In this paper, in the dry sand foundation, taking the Burbo Bank 3.6 MW offshore turbine-foundation structure as the prototype, the horizontal cyclic loading model tests of the wind power pile foundation with the scale of 1:50 were carried out. Considering the factors such as loading frequency and cyclic load ratio, the horizontal dynamic characteristics of the whole OWE pile foundation are studied. The comparison results between the maximum bending moment of pile and the fitting formula are discussed. In conclusion, moment of OWE pile shaft is corresponding to the loading frequency ( = 9 HZ) and loading cycles by fitting formulas. The fatigue damage of the OWE pile does not occurs with low frequencies in high cycles.
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http://dx.doi.org/10.3390/s20185348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570637PMC
September 2020

Exposure to microplastics cause gut damage, locomotor dysfunction, epigenetic silencing, and aggravate cadmium (Cd) toxicity in Drosophila.

Sci Total Environ 2020 Nov 15;744:140979. Epub 2020 Jul 15.

Program in Molecular and Integrative Physiological Sciences, Department of Environmental Health, Harvard T. H. Chan School of Public Health, Boston, MA 02115, USA; Broad Institute of Harvard and MIT, Cambridge, MA 02139, USA. Electronic address:

The interactions of microplastics (MPs) with other chemicals and the range of outcomes are of great importance to enhance understanding of their environmental impacts and health risks. Cadmium (Cd) and cadmium compounds are widely used as pigments and stabilizers in plastics, but they readily leach out. Here we addressed the impacts of MPs, Cd, and their joint exposure in a tractable Drosophila melanogaster model. We show that exposure to MPs lead to extensive particle size depended gut damage early in life and an enhancement of Cd-induced inhibition of locomotor-behavioral function in adult flies. In addition, we show that Cd exposure induces epigenetic gene silencing via position-effect variegation (PEV) in somatic tissues that was dramatically enhanced by co-exposure with MPs. The results indicate that MPs can aggravate the toxicity of other environmental contaminants and induce adverse effects across a range of diverse outcomes in a tractable and widely used model organism. These observations raise the prospects of using Drosophila as a tool for the rapid assessment of MP-mediated toxicity.
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http://dx.doi.org/10.1016/j.scitotenv.2020.140979DOI Listing
November 2020

Association between Adiponectin Gene Polymorphism and Environmental Risk Factors of Type 2 Diabetes Mellitus among the Chinese Population in Hohhot.

Biomed Res Int 2020 21;2020:6383906. Epub 2020 Jun 21.

Inner Mongolia Institute of Traditional Chinese Medicine, Hohhot, Inner Mongolia 010020, China.

Objective: The aim of this study was to investigate the association between adiponectin gene polymorphisms , , , , and and environmental risk factors of type 2 diabetes mellitus (T2DM) in Hohhot. The study explored different models of gene-environment interactions, aimed at providing approaches for the prevention and control of T2DM in combination with the characteristics of the local population.

Methods: A case-control study was conducted including 406 Chinese participants, comprising 203 cases and 203 controls from various hospitals. Adiponectin () gene polymorphisms , , , , and were detected using an improved multiple ligation detection reaction technique. Generalized multifactor dimensionality reduction (GMDR) and logistic regression were conducted to analyze the associations between adiponectin gene polymorphisms and T2DM, as well as the interactions between adiponectin gene polymorphisms and environmental factors.

Results: gene polymorphisms , , , , and were associated with type 2 diabetes. Based on the haplotype of the five adiponectin gene single-nucleotide polymorphism (SNP) loci, we found that G-G-A-A-C was a susceptible haplotype of T2DM ( < 0.05). Interaction analyses demonstrated associations between and central obesity (consistency = 80%, = 0.011) and between and and central obesity (consistency = 70%, = 0.011).

Conclusions: Our findings indicate that there is an interaction between the gene and central obesity, which provides new insights into the prevention and treatment of T2DM.
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http://dx.doi.org/10.1155/2020/6383906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327607PMC
April 2021

Use of Lung Ultrasound to Differentiate Coronavirus Disease 2019 (COVID-19) Pneumonia From Community-Acquired Pneumonia.

Ultrasound Med Biol 2020 10 5;46(10):2651-2658. Epub 2020 Jun 5.

Collaborative Innovation Center for Maternal and Infant Health Service Application Technology of Education Ministry, Quanzhou Medical College, Fujian, China; Department of Ultrasound Medicine, Second Affiliated Hospital of Fujian Medical University, Fujian, China; Department of Ultrasound Medicine, Zhangzhou Municipal Hospital, Fujian, China; Affiliated Hospital of Quanzhou Medical College, Quanzhou, China; Jinjiang Municipal Hospital, Fujian, China. Electronic address:

To investigate the feasibility of lung ultrasound in evaluating coronavirus disease 2019 (COVID-19) and distinguish the sonographic features between COVID-19 and community-acquired pneumonia (CAP), a total of 12 COVID-19 patients and 20 CAP patients were selected and underwent lung ultrasound. The modified Buda scoring system for interstitial lung disease was used to evaluate the severity and treatment effect of COVID-19 on ultrasonography. The differences between modified lung ultrasound (MLUS) score and high-resolution computed tomography (HRCT) Warrick score were analyzed to evaluate their correlation. COVID-19 showed the following sonographic features: thickening (12/12), blurred (9/12), discontinuous (6/12) pleural line; rocket sign (4/12), partially diffused B-line (12/12), completely diffused B-line (10/12), waterfall sign (4/12); C-line sign (5/12); pleural effusion (1/12) and pulmonary balloon (Am line, 1/12). The last two features were rarely seen. Differences of ultrasonic features, including lesion range, lung signs and pneumonia-related complications, between COVID-19 and CAP were statistically significant (p˂ 0.05 or 0.001). MLUS scores (p = 0.006) and HRCT Warrick scores (p = 0.015) increased as the severity of COVID-19 increased. The differences between moderate (29.00 [25.75-37.50]) and severe (43.00 [38.75-47.25]) (p = 0.022) or between moderate and critical (47.50 [44.25-50.00]) (p = 0.002) type COVID-19 were statistically significant, compared with those between severe and critical types. Correlation between MLUS scores and HRCT Warrick scores was positive (r = 0.54, p = 0.048). MLUS scores (Z = 2.61, p = 0.009) and HRCT Warrick scores (Z = 2.63, p = 0.009) of five severe or critical COVID-19 patients significantly decreased as their conditions improved after treatment. The differences of sonographic features between COVID-19 and CAP patients were notable. The MLUS scoring system could be used to evaluate the severity and treatment effect of COVID-19.
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http://dx.doi.org/10.1016/j.ultrasmedbio.2020.05.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7274602PMC
October 2020

Multiple approaches for massively parallel sequencing of SARS-CoV-2 genomes directly from clinical samples.

Genome Med 2020 06 30;12(1):57. Epub 2020 Jun 30.

BGI-Shenzhen, Shenzhen, 518083, China.

Background: COVID-19 (coronavirus disease 2019) has caused a major epidemic worldwide; however, much is yet to be known about the epidemiology and evolution of the virus partly due to the scarcity of full-length SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) genomes reported. One reason is that the challenges underneath sequencing SARS-CoV-2 directly from clinical samples have not been completely tackled, i.e., sequencing samples with low viral load often results in insufficient viral reads for analyses.

Methods: We applied a novel multiplex PCR amplicon (amplicon)-based and hybrid capture (capture)-based sequencing, as well as ultra-high-throughput metatranscriptomic (meta) sequencing in retrieving complete genomes, inter-individual and intra-individual variations of SARS-CoV-2 from serials dilutions of a cultured isolate, and eight clinical samples covering a range of sample types and viral loads. We also examined and compared the sensitivity, accuracy, and other characteristics of these approaches in a comprehensive manner.

Results: We demonstrated that both amplicon and capture methods efficiently enriched SARS-CoV-2 content from clinical samples, while the enrichment efficiency of amplicon outran that of capture in more challenging samples. We found that capture was not as accurate as meta and amplicon in identifying between-sample variations, whereas amplicon method was not as accurate as the other two in investigating within-sample variations, suggesting amplicon sequencing was not suitable for studying virus-host interactions and viral transmission that heavily rely on intra-host dynamics. We illustrated that meta uncovered rich genetic information in the clinical samples besides SARS-CoV-2, providing references for clinical diagnostics and therapeutics. Taken all factors above and cost-effectiveness into consideration, we proposed guidance for how to choose sequencing strategy for SARS-CoV-2 under different situations.

Conclusions: This is, to the best of our knowledge, the first work systematically investigating inter- and intra-individual variations of SARS-CoV-2 using amplicon- and capture-based whole-genome sequencing, as well as the first comparative study among multiple approaches. Our work offers practical solutions for genome sequencing and analyses of SARS-CoV-2 and other emerging viruses.
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http://dx.doi.org/10.1186/s13073-020-00751-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7325194PMC
June 2020

Assessment of somatosensory changes in Chinese temporomandibular disorders arthralgia patients by quantitative sensory testing.

J Oral Rehabil 2020 Sep 10;47(9):1129-1141. Epub 2020 Jul 10.

Department of Prosthodontics and Center for Oral Function Diagnosis, Treatment and Research, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing, China.

Background: Somatosensory changes in Chinese temporomandibular disorders (TMD) arthralgia patients have not been fully studied by the latest technologies.

Objective: This study aims at assessing somatosensory changes in Chinese TMD arthralgia patients quantitatively.

Methods: Standardised quantitative sensory testing (QST) was performed on the pain sites and contralateral sites of 40 patients diagnosed with TMD arthralgia according to the Diagnostic Criteria for Temporomandibular Disorders (DC/TMD) before and after medication; similar measures were taken in 40 age- and gender-matched healthy controls. Differences within and between groups were assessed through Z-scores, two-way ANOVA and loss/gain coding system.

Results: The pain sites of patients presented increased sensitivity to thermal stimuli and mechanical pain stimuli together with decreased sensitivity to mechanical tactile stimuli before medication (P < .001). Before treatment, 100% of patients had somatosensory abnormalities at the pain sites; the most frequent abnormalities were somatosensory gain to cold nociceptive, pinprick and pressure stimuli, and the most frequent loss/gain score was L0G3 (no somatosensory loss with a gain of thermal and mechanical somatosensory function; 70.0%). After treatment, although the clinical symptoms and signs of 40 patients disappeared, 80.0% of the patients' pain sites still showed multiple phenotype abnormalities. The most frequent loss/gain score was L0G2 (no somatosensory loss with a gain of mechanical somatosensory function; 35.0%).

Conclusions: Multiple phenotypes of facial somatosensory abnormalities were detected in Chinese TMD arthralgia patients before and after treatment, despite the disappearance of clinical signs and symptoms. Individual variations indicate a possible need for subgroup classification, individualised management and mechanism-based treatment.
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http://dx.doi.org/10.1111/joor.13038DOI Listing
September 2020

MicroRNA-126-5p suppresses cell proliferation, invasion and migration by targeting EGFR in liver cancer.

Clin Res Hepatol Gastroenterol 2020 11 12;44(6):865-873. Epub 2020 May 12.

The First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine, NO.1 Building, Yunbin Bay, Jianggan District, Hangzhou 310000, Zhejiang, China. Electronic address:

Background: The expression status and potential mechanistic involvement of microRNA (miR)-126-5p in hepatocellular carcinoma (HCC) are still elusive currently. Here we set out to address this issue both in cell lines and in patients' tissues.

Methods: The relative expression levels of endogenous miR-126-5p and epidermal growth factor receptor (EGFR) were quantified by real-time polymerase chain reaction. Cell viability and proliferation were measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and clonogenic assays, respectively. Cell invasive and migrative capacities were determined by transwell and wound healing assays, respectively. The regulatory action of miR-126-5p on EGFR was interrogated by luciferase reporter assay. Translational level of EGFR was analyzed by Western blotting.

Results: MiR-126-5p was significantly down-regulated in both HCC patients' tissues and cell lines. Forced expression of miR-126-5p greatly compromised cell viability, proliferation, invasion and migration, while miR-126-5p-specific inhibitor promoted these oncogenic phenotypes. MiR-126-5p mimics inhibited endogenous expression of EGFR and suppressed EGFR 3'-untranslated region-fused luciferase activity. Co-expression of EGFR in miR-126-5p-proficient cells completely restored cell migrative and invasive capacities, while co-transfection with EGFR siRNA significantly inhibited miR-126-5p inhibitor-induced cell invasion and migration.

Conclusion: MiR-126-5p was aberrantly decreased in HCC and subsequently relieved the suppression on EGFR expression, which consequently contributed to the tumor biology of HCC.
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http://dx.doi.org/10.1016/j.clinre.2020.03.025DOI Listing
November 2020

Comprehensive characterization of plasma cell-free Echinococcus spp. DNA in echinococcosis patients using ultra-high-throughput sequencing.

PLoS Negl Trop Dis 2020 04 13;14(4):e0008148. Epub 2020 Apr 13.

Second People's Hospital of Tibet Autonomous Region, Lhasa, China.

Background: Echinococcosis is a life-threatening parasitic disease caused by Echinococcus spp. tapeworms with over one million people affected globally at any time. The Echinococcus spp. tapeworms in the human body release DNA to the circulatory system, which can be a biomarker for echinococcosis. Cell-free DNA (cfDNA) is widely used in medical research and has been applied in various clinical settings. As for echinococcosis, several PCR-based tests had been trialed to detect cell-free Echinococcus spp. DNA in plasma or serum, but the sensitivity was about 20% to 25%. Low sensitivity of PCR-based methods might be related to our limited understanding of the features of cell-free Echinococcus spp. DNA in plasma, including its concentration, fragment pattern and release source. In this study, we applied ultra-high-throughput sequencing to comprehensively investigate the characteristics of cell-free Echinococcus spp. DNA in plasma of echinococcosis patients.

Methodology/principal Findings: We collected plasma samples from 23 echinococcosis patients. Total plasma cfDNA was extracted and sequenced with a high-throughput sequencing platform. An average of 282 million read pairs were obtained for each plasma sample. Sequencing data were analyzed with bioinformatics workflow combined with Echinococcus spp. sequence database. After identification of cell-free Echinococcus spp. reads, we found that the cell-free Echinococcus spp. reads accounted for 1.8e-5 to 4.0e-9 of the total clean reads. Comparing fragment length distribution of cfDNA between Echinococcus spp. and humans showed that cell-free Echinococcus spp. DNA of cystic echinococcosis (CE) had a broad length range, while that of alveolar echinococcosis (AE) had an obvious peak at about 135 bp. We found that most of the cell-free Echinococcus spp. DNA reads were from the nuclear genome with an even distribution, which might indicate a random release pattern of cell-free Echinococcus spp. DNA.

Conclusions/significance: With ultra-high-throughput sequencing technology, we analyzed the concentration, fragment length, release source, and other characteristics of cell-free Echinococcus spp. DNA in the plasma of echinococcosis patients. A better understanding of the characteristics of cell-free Echinococcus spp. DNA in plasma may facilitate their future application as a biomarker for diagnosis.
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http://dx.doi.org/10.1371/journal.pntd.0008148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7209354PMC
April 2020

Phytoremediation of soil heavy metals (Cd and Zn) by castor seedlings: Tolerance, accumulation and subcellular distribution.

Chemosphere 2020 Aug 19;252:126471. Epub 2020 Mar 19.

School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, PR China.

Cd and Zn pollution was observed to often occur simultaneously in soils. However, previous studies focused on single heavy metal instead of Cd and Zn combined pollution. Castor (Ricinus communis) is considered to have great potential for contaminated soil remediation. The resistance of castor seedlings to heavy metals and the mechanism behind it remain unknown. In this study, the tolerance and accumulation ability of castor seedlings to Cd and Zn were investigated, and the accumulation mechanism involving the subcellular distribution in different tissues was further explored. The results on biomass and chlorophyll revealed that castor seedlings have good tolerance to the pollution with 0-5 mg/kg Cd and 380 mg/kg Zn, while not to the heavy pollution with 25 mg/kg Cd and 380 mg/kg Zn. The maximum accumulation concentrations of Cd and Zn, 175.3 mg Cd/kg and 386.8 mg/kg Zn, appeared in castor seedling root instead of stem and leaf, indicating that root played a significant part in accumulating Zn and Cd. The relative low dosage of Cd (0-5 mg/kg) promoted the accumulation of Zn in the subcellular component, while high dosage (25 mg/kg) inhibited the accumulation of Zn. In subcellular accumulation and distribution of castor seedlings, Cd (27.1%-69.4%) and Zn (39.6%-66.6%) in the cell wall was the highest. With the increase of Cd addition, the accumulation of Cd increased in cell wall while decreased in organelle and soluble fraction. Hydroxyl, amino, amides and carboxyl functional groups on cell wall might provided the main binding sites for Cd and Zn.
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http://dx.doi.org/10.1016/j.chemosphere.2020.126471DOI Listing
August 2020

The safety and efficacy of airway pressure release ventilation in acute respiratory distress syndrome patients: A PRISMA-compliant systematic review and meta-analysis.

Medicine (Baltimore) 2020 Jan;99(1):e18586

Department of Critical Care Medicine, Chinese Medicine Hospital Changji Autonomous Prefecture, Changji, Xinjiang Uygur Autonomous Region, PR China.

Background: The acute respiratory distress syndrome (ARDS) is a critical illness with high mortality and a worse prognosis. Mechanical ventilation (MV) is currently considered to be one of the most effective methods of treating ARDS. In this meta-analysis, we discussed the efficacy of airway pressure release ventilation (APRV) in treating ARDS.

Methods: Following the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA), Ovid Medline, Embase, and PubMed were systematically searched with the keywords of "ARDS" and "APRV". The studies containing the treatment of APRV in ARDS were included. According to the MV protocol used in the studies, the comparison was undertaken between the APRV group vs low tidal volume (LTV) group and synchronized intermittent mandatory ventilation (SIMV) group. The relative risk (RR) and the standard mean difference with 95% confidence intervals (CI) were used for the comparison between groups.

Results: Fourteen studies with 2096 patients were included in the meta-analysis. The average increasing rate of PaO2/FiO2 was 75.4% in the APRV group vs 44.1% in the non-APRV group. No significant differences were found in mortality and duration of ICU stay between APRV vs LTV (P = .073 and P = .404) and APRV vs SIMV (P = .370 and P = .894).

Conclusion: The APRV protocol would have a higher increase in the PaO2/FiO2 ratio, which was a safe protocol with a compatible effect comparing to LTV and SIMV.
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http://dx.doi.org/10.1097/MD.0000000000018586DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946469PMC
January 2020

An efficient method for extracting microplastics from feces of different species.

J Hazard Mater 2020 02 23;384:121489. Epub 2019 Oct 23.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu, 210023, China. Electronic address:

Concerns have been raised regarding the ingestion of microplastics (MPs) by numerous organisms including humans. However, no efficient and standardized methods are available for extracting MPs from feces. In this study, we introduce a novel approach with high digestion efficiency that involves using Fenton's reagent and nitric acid to remove feces solids. Firstly, Fenton's reagent was used to degrade small solids and decompose large solids into small pieces. Secondly, nitric acid was used to digest the remaining solids and filters. Furthermore, absolute ethyl alcohol was used to remove the mineral residues wrapped on the plastic surfaces and disperse MPs. By using this method, 97.78 % MPs can be recovered from human and chicken feces, and no significant changes were observed in the physical and Raman spectral properties of different polymer types of MPs. This method has also been verified by extracting MPs from field feces. Overall, the proposed method can efficiently digest feces solids and extract MPs with higher recovery rate, less intermediate steps and less damage, which can serve as an economical and feasible method for the detection of MPs in the feces of different species.
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http://dx.doi.org/10.1016/j.jhazmat.2019.121489DOI Listing
February 2020

Icariin-induced miR-875-5p attenuates epithelial-mesenchymal transition by targeting hedgehog signaling in liver fibrosis.

J Gastroenterol Hepatol 2020 Mar 7;35(3):482-491. Epub 2019 Nov 7.

First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine, Zhejiang, China.

Background: Hepatic fibrosis is the final endpoint for most chronic liver diseases and remains a significant public health problem worldwide. Icariin, a naturally occurring flavonol glucoside, has been reported to exhibit protective effects on liver injury and alleviate liver fibrosis. However, the underlying detail molecular mechanism is not fully revealed.

Methods: Mouse primary hepatic stellate cells (HSCs) and carbon tetrachloride (CCL )-induced liver fibrosis model in mice were used as in vitro and in vivo models in this study. The expression levels of miR-875-5p were detected by quantitative reverse transcription-PCR. The validation of the direct target of miR-875-5p was through dual-luciferase reporter assay and western blotting assay. The cell proliferation and cell mobility were determined using MTT assay and Transwell migration assay, respectively.

Results: We found that icariin inhibited epithelial-mesenchymal transition and collagen protein section of HSCs. Icariin exerted hepatoprotective effects on mice model of CCL -induced liver fibrosis. Our further results revealed that miR-875-5p was downregulated in human cirrhosis tissues and activated murine HSCs. Icariin induced miR-875-5p upregulation and subsequently decreased glioma-associated oncogene homolog 1 (GLI1) expression through direct binding to the three prime untranslated region of GLI1 mRNA.

Conclusion: Our study highlighted the potential therapeutic application of icariin for liver fibrosis management.
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http://dx.doi.org/10.1111/jgh.14875DOI Listing
March 2020
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