Publications by authors named "Yanping Ma"

106 Publications

Assessment of genetic diversity and variety identification based on developed retrotransposon-based insertion polymorphism (RBIP) markers in sweet potato (Ipomoea batatas (L.) Lam.).

Sci Rep 2021 Aug 24;11(1):17116. Epub 2021 Aug 24.

Institute of Rural Development, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.

Sweet potato, a dicotyledonous and perennial plant, is the third tuber/root crop species behind potato and cassava in terms of production. Long terminal repeat (LTR) retrotransposons are highly abundant in sweet potato, contributing to genetic diversity. Retrotransposon-based insertion polymorphism (RBIP) is a high-throughput marker system to study the genetic diversity of plant species. To date, there have been no transposon marker-based genetic diversity analyses of sweet potato. Here, we reported a structure-based analysis of the sweet potato genome, a total of 21555 LTR retrotransposons, which belonged to the main LTR-retrotransposon subfamilies Ty3-gypsy and Ty1-copia were identified. After searching and selecting using Hidden Markov Models (HMMs), 1616 LTR retrotransposon sequences containing at least two models were screened. A total of 48 RBIP primers were synthesized based on the high copy numbers of conserved LTR sequences. Fifty-six amplicons with an average polymorphism of 91.07% were generated in 105 sweet potato germplasm resources based on RBIP markers. A Unweighted Pair Group Method with Arithmatic Mean (UPGMA) dendrogram, a model-based genetic structure and principal component analysis divided the sweet potato germplasms into 3 groups containing 8, 53, and 44 germplasms. All the three analyses produced significant groupwise consensus. However, almost all the germplasms contained only one primary locus. The analysis of molecular variance (AMOVA) among the groups indicated higher intergroup genetic variation (53%) than intrapopulation genetic variation. In addition, long-term self-retention may cause some germplasm resources to exhibit variable segregation. These results suggest that these sweet potato germplasms are not well evolutionarily diversified, although geographic speciation could have occurred at a limited level. This study highlights the utility of RBIP markers for determining the intraspecies variability of sweet potato and have the potential to be used as core primer pairs for variety identification, genetic diversity assessment and linkage map construction. The results could provide a good theoretical reference and guidance for germplasm research and breeding.
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http://dx.doi.org/10.1038/s41598-021-95876-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385064PMC
August 2021

EZH2/EHMT2 Histone Methyltransferases Inhibit the Transcription of DLX5 and Promote the Transformation of Myelodysplastic Syndrome to Acute Myeloid Leukemia.

Front Cell Dev Biol 2021 2;9:619795. Epub 2021 Aug 2.

Department of Hemapathotology, Second Hospital of Shanxi Medical University, Taiyuan, China.

Myelodysplastic syndrome (MDS) is characterized by clonal hematopoiesis and impaired differentiation, and may develop to acute myeloid leukemia (AML). We explored the mechanism of histone methyltransferase EZH2/EHMT2 during the transformation of MDS into AML. Expression of EZH2/EHMT2 in patients and NHD13 mice was detected. EZH2 and EHMT2 were silenced or overexpressed in SKM-1 cells. The cell proliferation and cycle were evaluated. Levels of DLX5, H3K27me3, and H3K9me2 in SKM-1 cells were detected. Binding of DLX5 promoter region to H3K27me3 and H3K9me2 was examined. Levels of H3K27me3/H3K9me2 were decreased by EZH2/EHMT2 inhibitor (EPZ-6438/BIX-01294), and changes of DLX5 expression and cell proliferation were observed. EZH2 was poorly expressed in MDS patients but highly expressed in MDS-AML patients. EHMT2 was promoted in both MDS and MDS-AML patients. EZH2 expression was reduced and EHMT2 expression was promoted in NHD13 mice. NHD13 mice with overexpressing EZH2 or EHMT2 transformed into AML more quickly. Intervention of EZH2 or EHMT2 inhibited SKM-1 cell proliferation and promoted DLX5 expression. When silencing EZH1 and EZH2 in SKM-1 cells, the H3K27me3 level was decreased. EZH2 silencing repressed the proliferation of SKM-1 cells. Transcription level of DLX5 in SKM-1 cells was inhibited by H3K27me3 and H3K9me2. Enhanced DLX5 repressed SKM-1 cell proliferation. In conclusion, EZH2/EHMT2 catalyzed H3K27me3/H3K9me2 to inhibit the transcription of DLX5, thus promoting the transformation from MDS to AML.
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http://dx.doi.org/10.3389/fcell.2021.619795DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365305PMC
August 2021

Thyroid dysfunction following radiofrequency ablation for benign thyroid nodules: more likely to occur within one-week and in high-risk population.

Int J Hyperthermia 2021 ;38(1):1060-1068

Department of Medical Ultrasonics, The Third Affiliated Hospital of Sun Yat-sen University, Guangdong Province Key Laboratory of Hepatology Research Guangzhou, Guangdong, China.

Background: Radiofrequency ablation (RFA) has been recommended as the treatment for benign thyroid nodules (BTNs) by some guidelines. However, detailed follow-up instructions for thyroid function about the timing and affected populations after RFA are lacked due to insufficient researches. This 12-month prospective study aimed to evaluate the incidence and risk factors of thyroid dysfunction at different time points after RFA, especially within 1 week that previous studies didn't concern.

Methods: Seventy-five euthyroid patients who underwent RFA for symptomatic BTNs were enrolled (ChiCTR-INR-16007884). The incidence of thyroid dysfunction within 1 week, at 1, 6, and 12 months after RFA was evaluated. The risk factors for different types of thyroid dysfunction in the different terms were further analyzed.

Results: Within 1 week after RFA, the incidence of thyroid dysfunction was as high as 36.00% unexpectedly, and only overt thyrotoxicosis and subclinical thyrotoxicosis occurred, which were significantly associated with the low-normal baseline thyrotropin (TSH) level ( = 0.001) and high ablation volume ratio ( = 0.008). From 1 to 12 months (the long term), the incidence dropped significantly and remained low (8.00-12.00%); and thyroid dysfunction presented as overt thyrotoxicosis, subclinical thyrotoxicosis, and subclinical hypothyroidism. The long-term thyrotoxicosis group had more cases with diabetes and lower baseline TSH levels. The long-term subclinical hypothyroidism group had more cases with positive thyroid peroxidase antibodies, higher baseline TSH levels, and higher ablation volume ratios.

Conclusions: After the RFA of BTNs, thyroid dysfunction was more likely to occur within 1 week and in populations with risk factors.
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http://dx.doi.org/10.1080/02656736.2021.1950849DOI Listing
August 2021

MIF-mediated NF-κB signaling pathway regulates the pathogenesis of polycystic ovary syndrome in rats.

Cytokine 2021 Oct 6;146:155632. Epub 2021 Jul 6.

Department of Reproduction and Genetics, Reproductive Medical Centre, The First People(,)s Hospital of Yunnan Province, 650000, China. Electronic address:

Polycystic ovary syndrome (PCOS) resulting from abnormal glucose metabolism is a relatively common and complex endocrine disorder among women in their reproductive years, However, the pathogenesis of PCOS is still unclear. The purpose of this study is to investigate the macrophage migration inhibitory factor (MIF) involvement of the nuclear factor (NF)-κB in rats with PCOS. Results indicated that testosterone promoted the increase in the levels of MIF and luteinizing hormone (LH) but inhibited the increase in the level of follicular stimulating hormone (FSH). The MIF antibody could alleviate the process of PCOS to a certain extent. Testosterone promoted the expression of interleukin 1-beta (IL-1β), interleukin 6 (IL-6), Inducible nitric oxide synthase (iNOS), and tumor necrosis factor alpha (TNF-α); the MIF antibody could reverse this effect. Testosterone could inhibit the expression of NF-κB protein whereas MIF antibody could promote the expression in the ovarian cytoplasm. Testosterone promoted the expression of NF-κB protein in the nucleus, this effect also could be reversed by the MIF antibody. Hyperandrogenism activated the NF-κB pathway. After using the MIF antibody, this effect was reversed. This finding suggested that hyperandrogenism activated the NF-κB pathway through MIF. In short, increased MIF levels activated the NF-κB pathway in ovaries, leading to inflammation and the increase in the levels of relevant inflammatory indicators, which might be one of the important factors in the pathogenesis of PCOS.
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http://dx.doi.org/10.1016/j.cyto.2021.155632DOI Listing
October 2021

Resin Transfer Moldable Fluorinated Phenylethynyl-Terminated Imide Oligomers with High T: Structure-Melt Stability Relationship.

Polymers (Basel) 2021 Mar 15;13(6). Epub 2021 Mar 15.

Key Laboratory of Engineering Plastics and Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Phenylethynyl-terminated aromatic polyimides meet requirements of resin transfer molding (RTM) and exhibits high glass transition temperature (T) were prepared. Moreover, the relationship between the polyimide backbones structure and their melting stability was investigated. The phenylethynyl-terminated polyimides were based on 4,4'-(hexafluorosiopropylidene)-diphthalic anhydride (6FDA) and different diamines of 3,4'-oxydianiline (3,4'-ODA), m-phenylenediamine (m-PDA) and 2,2'-bis(trifluoromethyl)benzidine (TFDB) were prepared. These oligoimides exhibit excellent melting flowability with wide processing temperature window and low minimum melt viscosities (<1 Pa·s). Two of the oligoimides display good melting stability at 280-290 °C, which meet the requirements of resin transfer molding (RTM) process. After thermally cured, all resins show high glass transition temperatures (Ts, 363-391 °C) and good tensile strength (51-66 MPa). The cure kinetics studied by the differential scanning calorimetry (DSC), C nuclear magnetic resonance (C NMR) characterization and density functional theory (DFT) definitely confirmed that the electron-withdrawing ability of oligoimide backbone can tremendously affect the curing reactivity of terminated phenylethynyl groups. The replacement of 3,4'-ODA units by m-PDA or TFDB units increase the electron-withdrawing ability of the backbone, which increase the curing rate of terminated phenylethynyl groups at processing temperatures, hence results in the worse melting stability.
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http://dx.doi.org/10.3390/polym13060903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999610PMC
March 2021

Transarterial chemoembolization (TACE) combined with apatinib versus TACE combined with sorafenib in advanced hepatocellular carcinoma patients: a multicenter retrospective study.

Ann Transl Med 2021 Feb;9(4):283

State Key Laboratory of Oncology in South China and Collaborative Innovation Center of Cancer Medicine, Sun Yat-sen University, Guangzhou, China.

Background: The combination of transarterial chemoembolization (TACE) with sorafenib has demonstrated superior efficacy over sorafenib and TACE monotherapy in hepatocellular carcinoma (HCC). Apatinib, a new targeted agent, has been recently reported to prolong the survival of HCC patients, either alone or in combination with TACE. However, the superior regimen between TACE-apatinib and TACE-sorafenib in HCC patients has not been determined. In this study, we compared the efficacy and safety of TACE-apatinib versus TACE-sorafenib in advanced stage HCC patients.

Methods: The data of 201 HCC patients who had received TACE-sorafenib or TACE-apatinib between January 2016 and June 2018 in three hospitals were retrospectively reviewed. Overall survival (OS), progression-free survival (PFS), and adverse effects (AEs) between the two treatment groups were compared. A subgroup analysis based on the doses of targeted agents was also performed.

Results: No significant differences in baseline clinicopathological features were found between the two groups except for dose reduction. The TACE-apatinib group had higher incidences of hypertension, oral or anal ulcer and proteinuria, while the TACE-sorafenib group had higher incidences of diarrhea and alopecia. Grade 3/4 AEs occurred more frequently in the TACE-apatinib group than in the TACE-sorafenib group (52.3% 22.6%, P<0.001). The TACE-sorafenib group had better PFS than the TACE-apatinib group (median PFS: 5.0 6.0 months, P=0.002) while the two groups showed no difference in OS (median OS: 13.0 13.0 months, P=0.448). The TACE-apatinib group had a higher rate of targeted agent dose reduction than the TACE-sorafenib group (53.5% 17.4%, P<0.001). When the patients were stratified into normal and reduced-dose subgroups, those who received TACE-sorafenib exhibited improved PFS but similar OS compared with the patients who received TACE-apatinib in the reduced-dose subgroup (median OS: 12.0 13.3 months, P=0.614; median PFS: 3.0 7.0 months, P<0.001). Multivariable analysis validated that treatments and dose reduction were independent prognostic factors for PFS among all patients.

Conclusions: Compared with TACE-sorafenib, the strategy of TACE-apatinib yielded shorter PFS in advanced HCC patients while no difference in OS was observed. A high rate of AE-related dose reduction of apatinib could account for the observed differences.
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http://dx.doi.org/10.21037/atm-20-5360DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944263PMC
February 2021

Virulence of "white-gray-opaque" tri-stable transformation in clinical Candida albicans in vitro and in vivo.

Microb Pathog 2021 May 6;154:104825. Epub 2021 Mar 6.

The Department of Dermatovenereology, The Second Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, China. Electronic address:

The study aimed to induce the white-opaque-gray tri-stable transformation in clinical C. albicans and to explore their potential pathogenicity. Sixty-four clinical strains were used to induce the white, opaque and gray cells of C. albicans. Secreted aspartyl proteinases (Sap) activity of the three phenotypes was then measured, and a vulvovaginal candidiasis (VVC) animal model was constructed. Of the 64 clinical strains, only 3 strains successfully underwent white-gray-opaque tri-stable transformation, and the three strains all belonged to MTL homozygous strains. Pz values in white, opaque and gray phenotypes were 0.834 ± 0.012, 0.707 ± 0.036, and 0.628 ± 0.002, respectively, which indicated that the cells with gray phenotype had higher Sap activity. After inoculation of different fungal suspension, the fungal colony count in descending order was as follows: gray phenotype, opaque phenotype and white phenotype. After treated with fluconazole for 3 days or 10 days, the fungal colony counts were significantly decreased compared with that before treatment (P < 0.05). The Sap activity and pathogenicity of gray cells in C. albicans were the strongest, followed by opaque cells and white cells. Additionally, white, gray and opaque phenotypic cells were all susceptible to fluconazole.
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http://dx.doi.org/10.1016/j.micpath.2021.104825DOI Listing
May 2021

Combination of High Zn Density and Low Phytic Acid for Improving Zn Bioavailability in Rice (Oryza stavia L.) Grain.

Rice (N Y) 2021 Feb 27;14(1):23. Epub 2021 Feb 27.

Institute of Rural Development, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.

Background: Zn deficiency is one of the leading public health problems in the world. Staple food crop, such as rice, cannot provide enough Zn to meet the daily dietary requirement because Zn in grain would chelate with phytic acid, which resulted in low Zn bioavailability. Breeding new rice varieties with high Zn bioavailability will be an effective, economic and sustainable strategy to alleviate human Zn deficiency.

Results: The high Zn density mutant LLZ was crossed with the low phytic acid mutant Os-lpa-XS110-1, and the contents of Zn and phytic acid in the brown rice were determined for the resulting progenies grown at different sites. Among the hybrid progenies, the double mutant always displayed significantly higher Zn content and lower phytic acid content in grain, leading to the lowest molar ratio of phytic acid to Zn under all environments. As assessed by in vitro digestion/Caco-2 cell model, the double mutant contained the relatively high content of bioavailable Zn in brown rice.

Conclusions: Our findings suggested pyramiding breeding by a combination of high Zn density and low phytic acid is a practical and useful approach to improve Zn bioavailability in rice grain.
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http://dx.doi.org/10.1186/s12284-021-00465-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914331PMC
February 2021

Enhancing Ethylene Polymerization of -Cobalt(II) Precatalysts Adorned with a Fluoro-substituent.

ACS Omega 2021 Feb 3;6(6):4448-4460. Epub 2021 Feb 3.

Key Laboratory of Engineering Plastics and Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Unsymmetrical 2-(1-(2,4-dibenzhydryl-6-fluorophenylimino)ethyl)-6-(1-alkylphenyl-imino)ethyl)pyridine compounds (Ar = 2,6-MeCH in ; 2,6-EtCH in ; 2,6- PrCH in ; 2,4,6-MeCH in ; 2,6-Et-4-Me-CH in ) were prepared and characterized. The treatment of CoCl with the compounds - afforded the corresponding cobalt complexes - in excellent yields. The molecular structures of and were determined by single-crystal X-ray diffraction, revealing the distorted-square-pyramidal geometry with three nitrogen atoms and two chlorine atoms around the cobalt center. Compared with previous bis(imino)pyridylcobalt analogues, all of the cobalt precatalysts displayed exceptionally higher activities toward ethylene polymerization with 1.32 × 10 g (PE) mol (Co) h at 60 °C in the presence of a co-catalyst MAO or MMAO. These cobalt catalysts produced highly linear polyethylene (PE) waxes with vinyl end groups and low molecular weight ( up to 8.23 kg mol) along with a relatively lower melting point (all-round s < 128 °C). The narrow dispersity of resultant polyethylenes indicated the single-site active species of the catalytic system.
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http://dx.doi.org/10.1021/acsomega.0c05916DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893795PMC
February 2021

Comparison of the Multiple Platforms to Identify Various Species.

Front Microbiol 2020 18;11:625961. Epub 2021 Jan 18.

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

We compared several identification methods for genus members, including traditional biochemical testing, multiplex-PCR amplification, mass spectrometry identification, whole-genome sequencing, multilocus phylogenetic analysis (MLPA), and , and - gene sequencing. Isolates ( = 62) belonging to the genus, which were came from the bacterial bank in the laboratory, were used to assess the identification accuracy of the different methods. Whole-genome sequencing showed that the spp. isolates comprised ( = 21), ( = 18), ( = 8), ( = 7), ( = 5), ( = 2), and ( = 1). Using the whole-genome sequencing results as the standard, the consistency of the other methods was compared with them. The results were 46.77% (29/62) for biochemical identification, 83.87% (52/62) for mass spectrometric identification, 67.74% (42/62) for multiplex-PCR, 100% (62/62) for MLPA typing, 72.58% for , and 59.68% for and -. MLPA was the most consistent, followed by mass spectrometry. Therefore, in the public health laboratory, both MLPA and whole-genome sequencing methods can be used to identify various species. However, rapid and relatively accurate mass spectrometry is recommended for clinical lab.
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http://dx.doi.org/10.3389/fmicb.2020.625961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848130PMC
January 2021

Characterization of a novel brain cell line from Jian carp (Cyprinus carpio var. Jian).

Fish Physiol Biochem 2021 Apr 6;47(2):439-449. Epub 2021 Jan 6.

College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.

Jian carp (Cyprinus carpio var. Jian) is an economically important cultured fish in China. Currently, it is facing threats from infectious diseases including koi herpesvirus (KHV). Here, we established a new cell line, designated CCB-J, derived from the brain tissue of the Jian carp. CCB-J cells grew well in Leibovitz's L-15 medium containing 20% fetal bovine serum at 25 °C and have been subcultured for more than 60 passages. At the 30th passage, analysis showed that the number of chromosomes was 100, which is identical to that of other carp variants. Sequencing of the 18S ribosomal DNA confirmed that CCB-J originated from Jian carp. After transfection with the pEGFP-N1 plasmid, green fluorescence was observed in CCB-J. The replication of KHV in CCB-J cells was confirmed by RT-PCR and transmission electron microscopy. The viral titers of KHV in CCB-J cells and CCB cells, which have been widely used in the study of KHV, reached 10 and 10 median tissue culture infectious dose (TCID/mL), respectively, within 14 days. The result of TaqMan PCR revealed that CCB-J cells were more sensitive to KHV than CCB cells. Meanwhile, a cytopathic effect (CPE) was also observed in the CCB-J cells in a shorter time post-infection compared with CCB cells. In summary, the CCB-J cell line will be a useful tool in the study of viral pathogenesis and vaccine research.
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http://dx.doi.org/10.1007/s10695-020-00923-4DOI Listing
April 2021

Unifying Molecular Weights of Highly Linear Polyethylene Waxes through Unsymmetrical 2,4-Bis(imino)pyridylchromium Chlorides.

Molecules 2020 Nov 27;25(23). Epub 2020 Nov 27.

Key Laboratory of Engineering Plastics and Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

By dealing CrCl∙3THF with the corresponding ligands (-), an array of fluoro-substituted chromium (III) chlorides (-) bearing 2-[1-(2,4-dibenzhydryl-6-fluoro- phenylimino)ethyl]-6-[1-(arylimino)ethyl]pyridine (aryl = 2,6-MePh , 2,6-EtPh , 2,6-iPrPh , 2,4,6-MePh , 2,6-Et-4-MePh ) was synthesized in good yield and validated via Fourier Transform Infrared (FT-IR) spectroscopy and elemental analysis. Besides the routine characterizations, the single-crystal X-ray diffraction study revealed the solid-state structures of complexes and as the distorted-octahedral geometry around the chromium center. Activated by either methylaluminoxane (MAO) or modified methylaluminoxane (MMAO), all the chromium catalysts exhibited high activities toward ethylene polymerization with the MMAO-promoted polymerizations far more productive than with MAO (20.14 × 10 g (PE) mol (Cr) h vs. 10.03 × 10 g (PE) mol (Cr) h). In both cases, the resultant polyethylenes were found as highly linear polyethylene waxes with low molecular weights around 1-2 kg mol and narrow molecular weight distribution (MWD range: 1.68-2.25). In general, both the catalytic performance of the -fluorinated chromium complexes and polymer properties have been the subject of a detailed investigation and proved to be highly dependent on the polymerization reaction parameters (including cocatalyst type and amount, reaction temperature, ethylene pressure and run time).
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http://dx.doi.org/10.3390/molecules25235584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7731025PMC
November 2020

Pregnancy outcome treated with stage-by-stage acupuncture and moxibustion therapy based on the chong channel being sea of blood theory in repeated IVF-ET failure patients: A randomized controlled trial.

Medicine (Baltimore) 2020 Nov;99(47):e23234

Yunnan First People's Hospital.

Introduction: Acupuncture and moxibustion has become a commonly used adjuvant treatment method to improve the success rate of in vitro fertilization-embryo transfer (IVF-ET). However, There is still insufficient evidence that acupuncture treatment can improve the local microenvironment of endometrium, the endometrial receptivity, and the pregnancy outcome of patients, which is worthy of further study.

Method/design: To investigate the effect of Stage by Stage Acupuncture and Moxibustion Therapy on endometrial receptivity and Pregnancy Outcome based on the theory of "Chong channel being sea of blood," we will conduct a multicenter randomized controlled trial. Inclusion criteria are as follows: infertile women under 45 years of age who received IVF-ET or Intracytoplasmic sperm injection cycles. The study will only be applied to women who have failed repeated implantation, that is, women who have failed 3 or more embryo transplants in the past (existing frozen embryos do not require the retrieval of eggs). Those who are not prepared to receive IVF-ET or are at risk of pregnancy, have a serious medical condition, or are egg donors will be excluded. Subjects will be randomly assigned to either the acupuncture group (IVF-ET plus stage-by-stage acupuncture and moxibustion therapy based on the "Chong channel being sea of blood" theory) or the control group (IVF-ET only). The trial required a total sample size of 246 women to compare endometrial receptivity between the 2 groups. The acupuncture group will receive acupuncture and moxibustion treatment 3 times a week starting from the third day of menstruation in the ovary stimulation cycle. One menstrual cycle was one course of treatment, and a total of 3 menstrual cycles were treated. The main outcome indicator was clinical pregnancy rate. Secondary outcome indicators were the three-dimensional volume blood flow parameters (vascularization index, flow index, and vascularization flow index) of the endometrium, endometrial thickness, endometrial volume, uterine artery PI, RI, and S/D during the "implantation window period" (20-24 days after menstruation in the ovary stimulation cycle).

Discussion: This study will provide important evidence for the use of Stage by Stage Acupuncture and Moxibustion Therapy Based on the "Chong Channel Being Sea of Blood" Theory in IVF.

Trial Registration: http://www.chictr.org.cn/edit.aspx?pid=28811&htm=4 ID: ChiCTR1800017191 (07/17/2018).
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http://dx.doi.org/10.1097/MD.0000000000023234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676521PMC
November 2020

Aza-crown compounds synthesised by the self-condensation of 2-amino-benzyl alcohol over a pincer ruthenium catalyst and applied in the transfer hydrogenation of ketones.

Dalton Trans 2020 Nov;49(44):15821-15827

Key Laboratory of Engineering Plastics and Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

A well-defined PNN-Ru catalyst was revisited to self-condense 2-aminobenzyl alcohol in forming a series of novel aza-crown compounds [aza-12-crown-3 (1), aza-16-crown-4 (2) and aza-20-crown-5 (3)]. All aza-crown compounds are separated and determined by NMR, IR, and ESI-MS spectroscopy as well as X-ray crystallography, indicating the saddle structure of 1 and the twisted 1,3-alternate conformation structure of 3. These aza-crown compounds have been explored to study ferric initiation of transfer hydrogenation (TH) of ketones into their corresponding secondary alcohols in the presence of 2-propanol with a basic t-BuOK solution, achieving a high conversion (up to 95%) by a ferric complex with 2 in a low loading (0.05 mol%).
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http://dx.doi.org/10.1039/d0dt03257cDOI Listing
November 2020

FilmArray GI-panel performance for the rapid and multiple detection of gastrointestinal microorganisms in foodborne illness outbreaks in Shenzhen during 2018-2019.

Infect Genet Evol 2020 12 23;86:104607. Epub 2020 Oct 23.

Department of Microbiological Laboratory, Shenzhen Nanshan Center for Disease Control and Prevention, 95#, Nanshang Road, Shenzhen, Guangdong 518054, China. Electronic address:

Foodborne illness outbreaks can be caused by a great many of gastrointestinal microorganisms including bacteria, viruses and parasites. Acute gastroenteritis is most commonly found in such patients infected with at least one pathogen through food intake. The stool culture has been conventionally used to guide a single diagnosis and therapy. However, traditional methods for identification of a pathogen are time-consuming and have limited sensitivity, leading to false negatives and co-infection omission. The aim of this study was to characterize the multiple etiology of each foodborne illness outbreak in Shenzhen during 2018-2019 by the FilmArray GI panel, and to reveal the seasonality of each causative organism incurring outbreaks. All patients included had a FilmArray GI panel performance and the seasonal characteristics were recorded. A total of 173 patients suffered from foodborne illnesses in 32 outbreaks in Nanshan District of Shenzhen. In total, 365 microorganisms were detected of which 83.8% (306/365) corresponded to bacteria and 16.2% (59/365) to viruses. Co-infections with more than one microorganism were detected in 81.3% (26/32) of the outbreaks. In 153 (88.4%) of 173 patients at least two pathogens were identified. The most common diarrheal pathogen related to outbreaks was EPEC (56%), followed by ETEC (38%), Norovirus (34%), EAEC (28%), Vibrio (25%), Salmonella (22%), P. shigelloides (22%), C. difficile (16%), STEC (3%) and Sapovirus (3%). Bacterial outbreaks occurred with a seasonal distribution with the exception of C. difficile whereas Norovirus outbreaks predominated during the autumn-winter months. The use of the FilmArray GI panel has given us worthy information regarding the epidemiology of pathogens detected in patients with acute diarrhea. It also highlights the importance of multi-pathogen infections and the frequency of diarrheogenic E. coli in foodborne disease outbreaks. More significantly, the rapid and multiple findings may help quickly taking an appropriate precaution, control and treatment.
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http://dx.doi.org/10.1016/j.meegid.2020.104607DOI Listing
December 2020

Which is better for mothers and babies: fresh or frozen-thawed blastocyst transfer?

BMC Pregnancy Childbirth 2020 Sep 23;20(1):559. Epub 2020 Sep 23.

Reproductive Medicine Center, The Fourth Affiliated Hospital of Jiangsu University (Zhenjiang Maternal and Child Health Hospital), 20 Zhengdong Road, Zhenjiang, Jiangsu, 212001, People's Republic of China.

Background: In recent years, there have been many reports on the pregnancy outcomes of fresh blastocyst transfer (BT) and frozen-thawed BT, but the conclusions are controversial and incomplete. To compare the pregnancy outcomes, maternal complications and neonatal outcomes of fresh and frozen-thawed BT in the context of in vitro fertilization or intracytoplasmic sperm injection (IVF/ICSI) cycles, we conducted a meta-analysis.

Methods: A meta-analysis was conducted by searching the PubMed, Embase, and Cochrane Library databases through May 2020. Data were extracted independently by two authors.

Results: Fifty-four studies, including 12 randomized controlled trials (RCTs), met the inclusion criteria. Fresh BT was associated with a lower implantation rate, pregnancy rate, ongoing pregnancy rate, and clinical pregnancy rate and higher ectopic pregnancy rate than frozen-thawed BT according to the results of the RCTs. The risks of moderate or severe ovarian hyperstimulation syndrome, placental abruption, placenta previa and preterm delivery were higher for fresh BT than for frozen-thawed BT. The risk of pregnancy-induced hypertension and pre-eclampsia was lower for fresh BT; however, no significant differences in risks for gestational diabetes mellitus and preterm rupture of membrane were found between the two groups. Compared with frozen-thawed BT, fresh BT appears to be associated with small for gestational age and low birth weight. No differences in the incidences of neonatal mortality or neonatal malformation were observed between fresh and frozen-thawed BT.

Conclusions: At present there is an overall slight preponderance of risks in fresh cycles against frozen, however individualization is required and current knowledge does not permit to address a defintive response.
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http://dx.doi.org/10.1186/s12884-020-03248-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7513314PMC
September 2020

6-Arylimino-2-(2-(1-phenylethyl)naphthalen-1-yl)-iminopyridylmetal (Fe and Co) Complexes as Highly Active Precatalysts for Ethylene Polymerization: Influence of Metal and/or Substituents on the Active, Thermostable Performance of Their Complexes and Resultant Polyethylenes.

Molecules 2020 Sep 16;25(18). Epub 2020 Sep 16.

Key Laboratory of Engineering Plastics and Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

A series of 6-arylimino-2-(2-(1-phenylethyl)naphthalen-1-yl)iminopyridines and their iron(II) and cobalt(II) complexes (-, -) were synthesized and routinely characterized as were and complexes, studied by single crystal X-ray crystallography, which individually displayed a distorted square pyramidal or trigonal bipyramid around a cobalt center. Upon treatment with either methyluminoxane (MAO) or modified methyluminoxane (MMAO), all complexes displayed high activities regarding ethylene polymerization even at an elevated temperature, enhancing the thermostability of the active species. In general, iron precatalysts showed higher activities than their cobalt analogs; for example, 10.9 × 10 g(PE) mol (Co) h by and 17.0 × 10 g(PE) mol (Fe) h by . Bulkier substituents are favored for increasing the molecular weights of the resultant polyethylenes, such as 25.6 kg mol obtained by and 297 kg mol obtained by . A narrow polydispersity of polyethylenes was observed by iron precatalysts activated by MMAO, indicating a single-site active species formed.
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http://dx.doi.org/10.3390/molecules25184244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570845PMC
September 2020

Human Cytomegalovirus Influences Host circRNA Transcriptions during Productive Infection.

Virol Sin 2021 Apr 5;36(2):241-253. Epub 2020 Aug 5.

Virology Laboratory, The Affiliated Shengjing Hospital, China Medical University, Shenyang, 110004, China.

Human cytomegalovirus (HCMV) is a double-strand DNA virus widely infected in human. Circular RNAs (circRNAs) are non-coding RNAs with most functions of which keep unknown, and the effects of HCMV productive infection on host circRNA transcriptions remain unclear. In this study, we profiled 283 host circRNAs that significantly altered by HCMV productive infection in human embryonic lung fibroblasts (HELF) by RNA deep sequencing and bioinformatics analysis. Among these, circSP100, circMAP3K1, circPLEKHM1, and circTRIO were validated for their transcriptions and sequences. Furthermore, characteristics of circSP100 were investigated by RT-qPCR and northern blot. It was implied that circSP100 was produced from the sense strand of the SP100 gene containing six exons. Kinetics of circSP100 and SP100 mRNA were significantly different after infection: circSP100 levels increased gradually along with infection, whereas SP100 mRNA levels increased in the beginning and dropped at 24 h post-infection (hpi). Meanwhile, a total number of 257 proteins, including 10 HCMV encoding proteins, were identified potentially binding to cytoplasmic circSP100 by RNA antisense purification (RAP) and mass spectrometry. Enrichment analysis showed these proteins were mainly involved in the spliceosome, protein processing, ribosome, and phagosome pathways, suggesting multiple functions of circSP100 during HCMV infection.
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http://dx.doi.org/10.1007/s12250-020-00275-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087738PMC
April 2021

Characterization of novel antigenic vaccine candidates for nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection.

Fish Shellfish Immunol 2020 Oct 24;105:405-414. Epub 2020 Jul 24.

Institute of Animal Health, Guangdong Academy of Agricultural Sciences; Key Laboratory of Livestock Disease Prevention of Guangdong Province; Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, PRC, Guangzhou, China. Electronic address:

Streptococcus agalactiae is one of the important pathogens responsible for high mortality and economic losses of the tilapia industry worldwide. Based on ten serovars of S. agalactiae infection, subunit vaccine with conserved antigens is promising strategy corresponding stimulated long-term immunity and provides protection for animals against different serotypes of S. agalactiae. In the present study, eight proteins (AP, AL, LivK, ESAT6, essA, essB, essC and esaA) were selected from the S. agalactiae serotype Ia genome as immunogenic antigens with bioinformation and immune experiment assays. These recombinant proteins were successfully obtained through expression in Escherichia coli and the immunogenicity was assessed in tilapia challenge model. The results showed that the recombinant proteins caused high-level-specific antibodies production and high lysozyme activities, suggesting that the recombinant proteins induced specific humoral immune response and innate immune response of tilapia. The signficant increase were observed in the cytokines levels of TNF-α, IL-1β, IFN-γ, cc1, cc2 and immune-related genes levels of CD8α and MHC factors in the spleen and head kidney tissues, suggesting that the recombinant proteins induced immune response of tilapia through cytokines signal pathway and activated high cytotoxic T-lymphocyte (CTL) activity of tilapia. Furthermore, vaccinated tilapia conferred high levels of protection against challenge with a lethal dose of highly virulent serovar Ⅰa (highest RPS was 91.60% in AL and essC protein groups). Our results indicated that the eight recombinant proteins induced high level of immune responses and offered protection against S. agalactiae infection, could be potential subunit vaccine candidates.
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http://dx.doi.org/10.1016/j.fsi.2020.07.024DOI Listing
October 2020

In vivo immunotoxicity of Gd O :Eu nanoparticles and the associated molecular mechanism.

J Biochem Mol Toxicol 2020 Nov 13;34(11):e22562. Epub 2020 Jul 13.

State Key Laboratory of Oncology in South China, Imaging Diagnosis and Interventional Center, Sun Yat-Sen University Cancer Center, Guangzhou, China.

The in vivo toxicity of Gd O :Eu nanoparticles (NPs) used as dual-modal nanoprobes for molecular imaging has not been studied, and the corresponding molecular mechanism of immunotoxicity remains unknown. In this study, we investigated the cytotoxicity, in vitro apoptosis, and in vivo immunotoxicity of Gd O :Eu NPs. The NPs showed little immunotoxicity to BALB/c mice. We explored the possible role of the phosphoinositide 3-kinase (PI3K) signaling pathway and found that reactive oxygen species could act as secondary messengers in cellular signaling, inhibiting PI3K expression in the liver. The immune suppression caused by PI3K inhibition helped the mice adapt to stress. The immunotoxicities caused by Gd O :Eu and gadodiamide, a commonly used contrast agent, were not significantly different, and the mice were able to tolerate the immunotoxicity caused Gd O :Eu NPs in vitro and in vivo experiments. The results suggest that Gd O :Eu NPs are sufficiently biocompatible to be used safely in preclinical applications and show promise as bio-imaging agents. Moreover, the in vivo molecular mechanism of immunotoxicity caused by the Gd O :Eu NPs provides a platform for further research on the immunotoxicity of nano-sized biomaterials.
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http://dx.doi.org/10.1002/jbt.22562DOI Listing
November 2020

Bis-cycloheptyl-fused bis(imino)pyridine-cobalt catalysts for PE wax formation: positive effects of fluoride substitution on catalytic performance and thermal stability.

Dalton Trans 2020 Jul;49(27):9425-9437

Key Laboratory of Engineering Plastics and Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. and CAS Research/Education Center for Excellence in Molecular Sciences and International School, University of Chinese Academy of Sciences, Beijing 100049, China and State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.

The α,α'-bis(imino)-2,3:5,6-bis(pentamethylene)pyridyl-cobalt(ii) chlorides, [2,3:5,6-{C4H8C(N(2-R1-4-R3-6-R2C6H2))}2C5HN] CoCl2 (R1 = Me, R2 = R3 = CH(p-FPh)2Co1; R1 = Et, R2 = R3 = CH(p-FPh)2Co2; R1 = i-Pr, R2 = R3 = CH(p-FPh)2Co3; R1 = Cl, R2 = R3 = CH(p-FPh)2Co4; R1 = F, R2 = R3 = CH(p-FPh)2Co5; R1 = F, R2 = R3 = CHPh2Co5'', R1 = R2 = Me, R3 = CH(p-FPh)2Co6; R1 = R3 = Me, R2 = CH(p-FPh)2Co7), have been synthesized by a one-pot template reaction of α,α'-dioxo-2,3:5,6-bis(pentamethylene)pyridine, cobalt(ii) chloride and the respective aniline in n-butanol. By contrast, the mixed cobalt(ii) chloride/acetate complex, [2,3:5,6-{C4H8C(N(2-F-4,6-(CH(p-FPh)2)2C6H2))}2C5HN]CoCl(OAc) (Co5'), was isolated when the corresponding template reaction was carried out in acetic acid. Structural characterization of Co4, Co5 and Co5'' revealed distorted square pyramidal geometries while six-coordinate Co5', incorporating a chelating acetate ligand, exhibited a distorted octahedral geometry. On activation with either MAO or MMAO, 2-fluoride-4,6-bis{di(p-fluorophenyl)methyl}-substituted Co5 showed maximum catalytic activity for ethylene polymerization at a high operating temperature of 60 °C (up to 2.1 × 107 g (PE) mol-1 (Co) h-1), producing highly linear (Tms > 121 °C), low molecular weight polyethylene waxes (Mw range: 1.5-5.0 kg mol-1) with narrow dispersity (Mw/Mn range: 1.7-2.9). End-group analysis of the waxes reveals β-H elimination as the dominant chain transfer process.
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http://dx.doi.org/10.1039/d0dt01876gDOI Listing
July 2020

Sterically and Electronically Modified Aryliminopyridyl-Nickel Bromide Precatalysts for an Access to Branched Polyethylene with Vinyl/Vinylene End Groups.

ACS Omega 2020 May 27;5(18):10610-10625. Epub 2020 Apr 27.

Key Laboratory of Engineering Plastics and Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

A series of 2-((arylimino)ethyl)pyridine derivatives (), each containing -2,4-bis(dibenzocycloheptyl) groups with variations in the steric/electronic properties of the ortho-substituent in the aryl ring, and the corresponding nickel bromide precatalysts [2-N{2,4-(CH)-6-R-CH}CHN]NiBr (R = Me (), Et (), Pr (), Cl (), or F ()), have been prepared in high yield. All the precatalysts are air-stable and characterized by Fourier transform infrared spectroscopy and elemental analysis. The molecular structures of and were proved through single-crystal X-ray diffraction analysis. The steric/electronic impact of the catalysts on ethylene polymerization and the resulting polymer properties were studied. Upon activation with either MAO or EASC, all the complexes displayed higher activities (up to 7.93 × 10 g of PE (mol of Ni) h with MAO) in ethylene polymerization and produced moderate to highly branched unsaturated polyethylene with a molecular weight of up to 16.55 kg/mol with narrow dispersities (1.6-2.4). Significantly, the generated polyethylenes are branched and unsaturated with a major class of internal double bond (-CH=CH-) as compared to the terminal double bond (-CH=CH) (vinylene/vinyl = 9.8:1 to 1.8:1). Notably, their catalytic activities, types of unsaturation, and branches are highly affected by the nature of the ortho-substituent and reaction temperature. Moreover, the precatalysts and (with N-ortho = Cl and F) exhibited lower catalytic activities, produced low-molecular-weight polyethylene with a high melt temperature and the least number of branches with an increased level of terminal double bonds.
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http://dx.doi.org/10.1021/acsomega.0c01208DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227053PMC
May 2020

Characterization of Human Cytomegalovirus UL16 and UL17 Transcripts.

New Microbiol 2020 Apr 19;43(2):58-63. Epub 2020 Apr 19.

Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 110004, China.

Up to now, the UL16-17 region of human cytomegalovirus (HCMV) has not been well characterized at the level of mRNA and protein, especially for the Han strain, the first clinical HCMV strain in China. In previous studies, three transcripts were detected from the UL16-17 region by northern blot analysis for Merlin strain. Transcriptions of UL16 and UL17 were also studied by 5' rapid amplification of cDNA ends (5'RACE) and deep sequencing for AD169 and Towne strains, respectively. However, details of 3' end of UL16 and UL17 transcripts have never been confirmed by 3'RACE. The expressing phage of the UL16-17 region needs further research by northern blot, too. In the present study, cDNA library screening, northern blot and RACE were used to identify the transcription characteristics of the UL16-17 region. Mainly, 3 clusters of transcripts with the same 3' end were found to be expressed from the UL16-17 region in both Han and AD169 strains. The lengths of the core transcripts among the 3 clusters were 1,254nt, 718nt and 468nt, respectively. The corresponding 5' ends are at nt23119, nt23655, nt23905 in the HCMV Han genome. The consistent 3' end is located at nt24372 in the Han genome. The 1,254nt and 468nt transcripts are transcribed in early and late phases, and the 718nt transcript is transcribed only in the late phase.
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April 2020

High molecular weight polyethylenes of narrow dispersity promoted using bis(arylimino)cyclohepta[b]pyridine-cobalt catalysts ortho-substituted with benzhydryl & cycloalkyl groups.

Dalton Trans 2020 Apr;49(15):4774-4784

Key Laboratory of Engineering Plastics and Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. and CAS Research/Education Center for Excellence in Molecular Sciences and International School, University of Chinese Academy of Sciences, Beijing 100049, China and State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.

A one-pot template strategy has been utilized to synthesize sterically enhanced bis(imino)cyclohepta[b]pyridine-cobalt(ii) chlorides, [2-{(Ar)N[double bond, length as m-dash]CMe}-9-{N(Ar)}C10H10N]CoCl2 (Ar = 2-(C5H9)-4,6-(CHPh2)2C6H2Co1, 2-(C6H11)-4,6-(CHPh2)2C6H2Co2, 2-(C8H15)-4,6-(CHPh2)2C6H2Co3, 2-(C12H23)-4,6-(CHPh2)2C6H2Co4, 2,6-(C5H9)2-4-(CHPh2)C6H2Co5). All five complexes have been characterized by a combination of FT-IR spectroscopy, elemental analysis and single crystal X-ray diffraction. The molecular structures of Co1, Co3 and Co5 highlight the substantial steric hindrance imparted by the 2-cycloalkyl-6-benzhydryl or 2,6-dicyclopentyl ortho-substitution pattern; distorted square pyramidal geometries are exhibited in each case. On activation with methylaluminoxane (MAO) or modified methylaluminoxane (MMAO), all the complexes (apart from Co4/MAO) were active ethylene polymerization catalysts (up to 3.70 × 106 g PE per mol (Co) per h for Co5/MMAO), operating effectively at temperatures between 50 °C and 60 °C, producing polyethylenes with high molecular weights (up to 589.5 kg mol-1 for Co3/MAO). Furthermore, all polymers were highly linear (Tm > 130 °C) with narrow dispersities (Mw/Mn range: 2.0-3.0). The coexistence of two chain termination pathways, β-H elimination and transfer to aluminum, has been demonstrated using 13C/1H NMR spectroscopy.
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http://dx.doi.org/10.1039/d0dt00576bDOI Listing
April 2020

Oral vaccination using Artemia coated with recombinant Saccharomyces cerevisiae expressing cyprinid herpesvirus-3 envelope antigen induces protective immunity in common carp (Cyprinus carpio var. Jian) larvae.

Res Vet Sci 2020 Jun 7;130:184-192. Epub 2020 Mar 7.

Guangdong Ocean University, Zhanjiang 524088, China. Electronic address:

Cyprinid herpesvirus 3 (CyHV-3) is the etiological agent of koi herpersvirus disease (KHVD), which causes serious economic losses in global common carp and ornamental koi carp production of larvae as well as adult type fish. To control KHVD, vaccines against CyHV-3 utilizing different immunization routes have been developed, among them, oral vaccination is the most desirable method to prevent fish diseases occurring at the early larval stage. Here, we developed an oral subunit vaccine through the Saccharomyces cerevisiae cell surface display of CyHV-3 envelope protein pORF65, then, the recombinant yeast fed to Artemia which served as bio-encapsulation vector by subsequently feeding the common carp (Cyprinus carpio var. Jian) larvae. The fluorescent observation showed that the Artemia and S. cerevisiae could deliver intact antigen to the hindgut of carp larvae suggesting the possibility of the vector for oral immunization. On this basis, after three immunizations at a week interval, the oral vaccine induced high level of specific anti-pORF65 antibody. Meanwhile, a significant difference of immune-related genes expression occurred including cxca, IL-1β, IFN-a1, lysozyme, IgM and CD8α between vaccined group and blank control group. In addition, 30% of relative percent survival of carp larvae after immunization was obtained post the animal infection assay, offered an certain immune protection. Our results indicated that the oral pORF65 subunit vaccine bioencapsulated in Artemia induced the activation of immune response and high level of antibodies, which could be served as an oral vaccine candidate for the prevention of CyHV-3 infection.
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http://dx.doi.org/10.1016/j.rvsc.2020.03.013DOI Listing
June 2020

Protective immunity against CyHV-3 infection via different prime-boost vaccination regimens using CyHV-3 ORF131-based DNA/protein subunit vaccines in carp Cyprinus carpio var. Jian.

Fish Shellfish Immunol 2020 Mar 22;98:342-353. Epub 2020 Jan 22.

Guangdong Ocean University, Zhanjiang, 524088, China. Electronic address:

Cyprinid Herpesvirus 3 (CyHV-3), also known as Koi Herpesvirus (KHV), causes Koi Herpesvirus Disease (KHVD) which leads to serious economic losses worldwide. To exploit DNA/subunit vaccine candidates, CyHV-3 ORF131 gene and cDNA was cloned and analyzed in the present study. Major B cell epitopes of deduced CyHV-3 pORF131 was also predicted. Then the complete CDS of CyHV-3 ORF131 was inserted into pEGFP-N1 vector and a modified pYD1/EBY100 system to construct the DNA and subunit vaccine, respectively. Subsequently, carp were immunized with homologous and heterologous prime-boost regimens relying on the constructed DNA and oral subunit vaccines. Then the protective immunity generated from different vaccines and regimens as well as the capacity of yeast (Saccharomyces cerevisiae) as an oral vaccine vehicle was evaluated. Our study confirmed that CyHV-3 ORF131 gene consisted of 2 introns and 3 exons encoding a 428 amino acids peptide. Further analysis indicated that four fragments of CyHV-3 pORF131 contained the major B cell epitopes (Cys20~Val140, Ser169~Tyr245, Thr258~Pro390, Phe414~Gln428), which could be linked and expressed in E. coli (BL21) as a truncated pORF131. The expression of full-length CyHV-3 pORF131 by pEGFP-N1 and yeast surface display was verified by In vitro assays before vaccination. Immunization of carp with CyHV-3 ORF131 DNA and subunit vaccines could evoke the activation of immune-related genes such as CXCa, CXCR1, IL-1β, TNF-α, INF-a1, Mx-1, IgM, IgT1 and production of specific serum IgM measured by ELISA. RPS (relative percent of survival) ranging from 53.33% to 66.67% was acquired post challenge test. Moreover, flow cytometry analysis illustrated the delivery of surface-displayed CyHV-3 pORF131 to midgut after oral gavage. Thus, our findings suggest that CyHV-3 ORF131 can serve as DNA/subunit vaccines candidate and the yeast as an ideal oral vaccine vehicle.
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http://dx.doi.org/10.1016/j.fsi.2020.01.034DOI Listing
March 2020

Diagnostic and prognostic significance of serum miR-203 in patients with acute myeloid leukemia.

Int J Clin Exp Pathol 2019 1;12(5):1548-1556. Epub 2019 May 1.

The Second Hospital of Shanxi Medical University Taiyuan 030001, Shanxi Province, China.

MicroRNAs play important roles in the initiation and progression of acute myeloid leukemia (AML). This study aimed to detect serum miR-203 expression levels in AML and explore its potential clinical significance. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to measure the serum miR-203 levels in 134 patients with AML and 70 healthy controls. The results demonstrated that serum miR-203 expression was significantly reduced in AML patients compared with healthy controls. Receiver operating characteristic curve (ROC) analysis revealed miR-203 could distinguish AML cases from normal controls. Low serum miR-203 levels were associated with worse clinical features, as well as poorer overall survival and relapse free survival of AML patients. Moreover, multivariate analysis confirmed low serum miR-203 expression to be an independent unfavorable prognostic predictor for AML. The bioinformatics analysis showed that the downstream genes and pathways of miR-203 was closely associated with tumorigenesis. Downregulation of miR-203 in AML cell lines upregulated the expression levels of oncogenic promoters such as CREB1, SRC and HDAC1. Thus, these findings demonstrated that serum miR-203 might be a promising biomarker for the diagnosis and prognosis of AML.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947105PMC
May 2019

Fusing Carbocycles of Inequivalent Ring Size to a Bis(imino)pyridine-Iron Ethylene Polymerization Catalyst: Distinctive Effects on Activity, PE Molecular Weight, and Dispersity.

Research (Wash D C) 2019 16;2019:9426063. Epub 2019 Oct 16.

Key Laboratory of Engineering Plastics and Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

The 4,6-bis(arylimino)-1,2,3,7,8,9,10-heptahydrocyclohepta[]quinoline-iron(II) chlorides (aryl = 2,6-MeCH ; 2,6-EtCH ; 2,6--PrCH ; 2,4,6-MeCH ; and 2,6-Et-4-MeCH ) have been prepared in good yield by a straightforward one-pot reaction of 2,3,7,8,9,10-hexahydro-1H-cyclohepta[]quinoline-4,6-dione, FeCl·4HO, and the appropriate aniline in acetic acid. All ferrous complexes have been characterized by elemental analysis and FT-IR spectroscopy. In addition, the structure of has been determined by single crystal X-ray diffraction, which showed the iron center to adopt a distorted square pyramidal geometry with the saturated sections of the fused six- and seven-membered carbocycles to be -configured. In combination with either MAO or MMAO, - exhibited exceptionally high activities for ethylene polymerization (up to 15.86 × 10 g(PE) mol (Fe) h at 40°C (MMAO) and 9.60 × 10 g(PE) mol (Fe) h at 60°C (MAO)) and produced highly linear polyethylene (HLPE, ≥ 128°C) with a wide range in molecular weights; in general, the MMAO-promoted polymerizations were more active. Irrespective of the cocatalyst employed, the 2,6-Me-substituted and proved the most active while the more sterically hindered 2,6--Pr the least but afforded the highest molecular weight polyethylene ( 65.6-72.6 kg mol). Multinuclear NMR spectroscopic analysis of the polymer formed using /MMAO at 40°C showed a preference for fully saturated chain ends with a broad bimodal distribution a feature of the GPC trace ( / = 13.4). By contrast, using /MAO at 60°C a vinyl-terminated polymer of lower molecular weight ( = 14.2 kg mol) was identified that exhibited a unimodal distribution ( / = 3.8). Moreover, the amount of aluminoxane cocatalyst employed, temperature, and run time were also found to be influential on the modality of the polymer.
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http://dx.doi.org/10.34133/2019/9426063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946285PMC
October 2019

Highly efficient WO-FeO catalysts synthesized using a novel solvent-free method for NH-SCR.

J Hazard Mater 2020 Apr 3;388:121812. Epub 2019 Dec 3.

Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, PR China. Electronic address:

WO-FeO catalysts with various WO contents were synthesized through a facile solvent-free method, satisfying the selective catalytic reduction of NO (NH-SCR). Strikingly, the optimum 30 %WO-FeO catalyst with the largest surface area exhibited the most outstanding catalytic activity, achieving the nearly 100 % NO removal efficiency in a wide temperature window between 225-500 °C, which was better than that of Fe-W series catalysts reported in other studies. In addition, Raman and XPS results proved that the introduction of WO altered the electronic environment of FeO, inducing the formation of FeO (Fe) and surface adsorbed oxygen. In situ DRIFTS demonstrated that the interaction between WO and FeO not only promoted the adsorption capacity of NH on the catalyst, but also contributed to the formation of adsorbed NO species. NO reduction reaction on WO-FeO catalyst proceeded via the Eley-Rideal and Langmuir-Hinshelwood mechanism synchronously. All of these factors, jointly, accounted for the superior catalytic activity and N selectivity of WO-FeO catalysts.
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http://dx.doi.org/10.1016/j.jhazmat.2019.121812DOI Listing
April 2020
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