Publications by authors named "Yanping Huang"

113 Publications

and correlation for lipid-based formulations: Current status and future perspectives.

Acta Pharm Sin B 2021 Aug 21;11(8):2469-2487. Epub 2021 Mar 21.

Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai 200443, China.

Lipid-based formulations (LBFs) have demonstrated a great potential in enhancing the oral absorption of poorly water-soluble drugs. However, construction of and correlations (IVIVCs) for LBFs is quite challenging, owing to a complex processing of these formulations. In this paper, we start with a brief introduction on the gastrointestinal digestion of lipid/LBFs and its relation to enhanced oral drug absorption; based on the concept of IVIVCs, the current status of models to establish IVIVCs for LBFs is reviewed, while future perspectives in this field are discussed. tests, which facilitate the understanding and prediction of the performance of solid dosage forms, frequently fail to mimic the processing of LBFs, leading to inconsistent results. digestion models, which more closely simulate gastrointestinal physiology, are a more promising option. Despite some successes in IVIVC modeling, the accuracy and consistency of these models are yet to be validated, particularly for human data. A reliable IVIVC model can not only reduce the risk, time, and cost of formulation development but can also contribute to the formulation design and optimization, thus promoting the clinical translation of LBFs.
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http://dx.doi.org/10.1016/j.apsb.2021.03.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424225PMC
August 2021

Customized eye modeling for optical quality assessment in myopic femto-LASIK surgery.

Sci Rep 2021 Aug 6;11(1):16049. Epub 2021 Aug 6.

School of Physics and Optoelectronic Engineering, Foshan University, Foshan, 528000, Guangdong, China.

Refractive surgery is recognized as an effective method for myopia treatment, but it can induce night vision disturbances such as glare. We present an eye modeling method for the optical quality assessment in response to the structural changes in the eyes by femto-LASIK surgery. Customized eye models were built from the measurements of 134 right eyes pre- and post-operatively. Optical performance was evaluated using spot diagrams, point spread functions (PSFs), modulation transfer functions (MTFs), and chromatic aberrations at various fields (0°-30°), different pupil diameters (2-6 mm), and initial myopias (- 1.25 to - 10.5 D). Pupil size and initial myopia are the two major factors that affect visual performance of post-operative eyes. The results of spot diagrams, PSFs, and MTFs indicated that post-operative visual performance deteriorated as the visual field and pupil size increased, and it was significantly influenced by initial myopia. Post-operative chromatic aberrations were also affected by initial myopia. As pupil size increased, the post-operative longitudinal chromatic aberrations tended to decrease slightly, while the transverse chromatic aberrations remained similar. The use of eye modeling for refractive surgery assessment could possibly provide a more personalized surgical approach, could improve the prediction accuracy of refractive surgery outcomes, and promote the invention and development of better surgical methods.
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http://dx.doi.org/10.1038/s41598-021-95730-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346559PMC
August 2021

[N-acetylcysteine inhibits the proliferation of hydrogen peroxide treated fibroblast-like synoviocytes in rats with adjuvant arthritis (AA) via blocking Nrf2/Keap1 pathway].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Aug;37(8):687-692

Experimental Center of Morphology, Anhui Medical University, Hefei 230032, China. *Corresponding author, E-mail:

Objective To investigate the effect of N-acetylcysteine (NAC) on the proliferation of fibroblast-like synoviocytes (FLS) treated with low concentration of hydrogen peroxide (HO) in rats with adjuvant arthritis (AA) and its mechanism. Methods Twenty SD rats were divided into a normal group and a model group (10 rats in each group). The model group was established by subcutaneous injection of Freund's complete adjuvant into the toe of rats, and the rats were sacrificed 28 days later. The contents of serum malondialdehyde (MDA) were detected by thiobarbituric acid method; the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were determined by hydroxylamine method and colorimetry respectively; and Nrf2 and Keap1 proteins in ankle synovial tissues of AA rats were detected by immunohistochemistry. AA-FLS were isolated, cultured, and identified by digestion of ankle joint slides of AA rats in vitro. The effects of NAC at different concentrations (final concentration 0, 0.3, 0.9, 3, 10, 30, 90, 180 μmol/L) on the activity of AA-FLS treated with HO at low concentration (5 μmol/L) were detected by CCK-8 assay. The content of mitochondrial reactive oxygen species (ROS) in AA-FLS was detected by MitoSOX fluorescent probe. The effects of NAC (final concentration 0, 3, 10, 30 μmol/L) on Nrf2 and Keap1 protein expressions in AA-FLS treated with HO at low concentration were detected by Western blotting. Results Compared with those in the control group, in AA model, the MDA level increased and SOD and GSH-Px levels decreased in serum, and the Nrf2 protein increased and the Keap1 protein decreased in synovial tissue. Immunocytochemical staining confirmed that the isolated and cultured cells were AA-FLS; NAC inhibited the proliferation of AA-FLS treated with HO in a concentration-dependent manner, and the mitochondrial ROS content and the protein expressions of Nrf2 and Keap1 decreased. Conclusion NAC can inhibit the proliferation of AA-FLS treated with HO, which may be related to blocking Nrf2/Keap1 pathway.
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August 2021

Wrinkle and near-resonance effects on the vibrational and electronic properties in compressed monolayer MoSe.

Phys Chem Chem Phys 2021 May 13;23(20):11709-11716. Epub 2021 May 13.

State Key Laboratory of Superhard Materials, College of Physics, Jilin University, Changchun, 130012, China.

Pressure has been considered as an effective technique to modulate the structural, electronic, and optical properties of transition metal dichalcogenide (TMDs) materials. Here, by performing in situ high pressure Raman, photoluminescence (PL) and absorption measurements, we systematically investigated the vibrational and electronic properties evolution of monolayer MoSe grown on a SiO/Si substrate under high pressure. When the pressure increased up to 4.84 GPa, an unexpected phonon mode at 367 cm appeared, which was identified as the Raman-inactive A'' mode and was activated under high pressure. Combined with the analysis of absorption spectroscopy, this phenomenon can be attributed to the pressure-induced wrinkle and near-resonance effects in compressed monolayer MoSe. Subsequently, A' split into two peaks after 7.44 GPa, providing further distinct evidence for the pressure-induced wrinkle effect in compressed monolayer MoSe. Moreover, this wrinkle effect can also lead to a rapid quenching of photoluminescence in monolayer MoSe. These results suggest that the substrate plays an important role in determining the vibrational and electronic properties of compressed monolayer MoSe, and can provide valuable information on the electronic and optoelectronic applications of monolayer MoSe under extreme conditions.
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http://dx.doi.org/10.1039/d0cp06283aDOI Listing
May 2021

A New Superconducting 3R-WS Phase at High Pressure.

J Phys Chem Lett 2021 Apr 26;12(13):3321-3327. Epub 2021 Mar 26.

State Key Laboratory of Superhard Materials, College of Physics, Jilin University, Changchun 130012, China.

High-pressure investigation has been shown to be of paramount significance for changing the conventional lattice or bringing fascinating properties, especially inducing superconducting phases. Here we studied the application of pressure to the recently synthesized 2M-WS with the record (8.8 K) among transition metal dichalcogenides (TMDs) at ambient pressure by electrical resistance investigations, synchrotron X-ray studies, and theoretical calculations. in the initial 2M-WS dropped from the maximum to become undetected, accompanied by a phase transition into a semiconductor, 3R-WS, at 15 GPa. The successive metallization and superconducting transitions in 3R-WS were observed at 48.8 GPa with ≈ 2.5 K. This is the first experimental case in which superconductivity has been realized in the 3R phase among TMDs. We propose that the degradation of superconductivity in 2M-WS and the reemergence of superconductivity in 3R-WS are mainly attributable to changes in the density of states near the Fermi surface driven by the interlayer coupling.
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http://dx.doi.org/10.1021/acs.jpclett.1c00312DOI Listing
April 2021

Mesoporous Palladium-Boron-Sulfur Alloy Nanospheres for Efficient Hydrogen Evolution.

Inorg Chem 2021 Apr 12;60(7):4380-4384. Epub 2021 Mar 12.

Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.

Ternary noble metal-metalloid-nonmetal PdBS alloy mesoporous nanospheres (MSs) with three-dimensional central-radial pore channels were prepared for an electrocatalytic hydrogen evolution reaction. The synthesis was performed via precise control in the reduction and nucleation growth of ternary PdBS alloy MSs along confined cylinder mesophases assembled by amphiphilic dioctadecyldimethylammonium chloride. The resultant PdBS alloy MSs disclosed a remarkably improved electrocatalytic performance due to their structural and compositional synergies. This finding extended our knowledge on the rational design and targeted synthesis of novel noble metal-metalloid-nonmetal alloys with desired structures and morphologies for catalysis and other applications.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00501DOI Listing
April 2021

Combination of Microneedles and MF59 Adjuvant as a Simple Approach to Enhance Transcutaneous Immunization.

J Biomed Nanotechnol 2020 Dec;16(12):1776-1786

MF59, an oil-in-water nanoemulsion, has been used in licensed seasonal influenza vaccines for many years. Administration of such vaccines by injection is associated with pain and safety issues. Here, we evaluated the potential of administering MF59 via a transcutaneous route with antigen loading (either encapsulated into or mixed with MF59) to intact or microneedle-pretreated skin. In addition to commercial MF59, we also prepared a nanoemulsion to encapsulate hydrophilic antigens by mimicking the formulation and preparation technique of MF59. The nanoemulsion was prepared using a water-in-oil-in-water emulsion method, and was similar to MF59 in composition, particle size, and morphology. Compared with the intact skin group, the microneedle-pretreated group showed significant enhanced antigen penetration. transcutaneous immunization analysis showed that the MF59-adjuvant influenza vaccine elicited approximately 3-5 times higher hemagglutination inhibition titers than the influenza solution alone in BALB/c mice after microneedle pretreatment. The intact skin group showed negative immune results at the same dose, suggesting that microneedle pretreatment was critical for efficient delivery of antigens, to obtain strong immune responses. Furthermore, the loading method (encapsulation or mixing with the vehicle) did not affect the dermal penetration or transcutaneous immunization of antigens on microneedle-pretreated skin. Moreover, cellular assays showed that MF59 facilitated the maturation of dendritic cells and enhanced antigen uptake by antigen-presenting cells. In conclusion, the combination of microneedle pretreatment and mixing of MF59 with antigen provides a simple approach to enhance transcutaneous immunization.
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http://dx.doi.org/10.1166/jbn.2020.3007DOI Listing
December 2020

Trimetallic Zeolitic imidazolite framework-derived Co [email protected] porous carbon as bifunctional electrocatalysts for Zn-air battery.

J Colloid Interface Sci 2021 Mar 3;586:621-629. Epub 2020 Nov 3.

Department of Materials Science and Engineering, Shenzhen Key Laboratory of Hydrogen Energy, Guangdong Provincial Key Laboratory of Energy Materials for Electric Power, Southern University of Science and Technology, Shenzhen 518055, PR China. Electronic address:

Searching for high active, low cost and durable catalysts for oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) remains challenge in developing metal-air battery cathodes. Herein, we proposed a novel bifunctional catalyst derived from the pyrolysis of Co/Fe/Zn trimetallic zeolitic imidazolite framework. The obtained [email protected] catalyst displays desirable activity for both ORR and OER (E = 0.844 V, E = 1.654 V). The Zn-air battery equipped with [email protected] catalyst on the cathode exhibits a high peak power density of 174.1 mW cm, which is much superior than that of commercial 20% Pt/C (87.6 mW cm). Significantly, the designed [email protected] presents an outstanding stability for over 100 h in rechargeable Zn-air battery.
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http://dx.doi.org/10.1016/j.jcis.2020.10.130DOI Listing
March 2021

Cancer and stress: NextGen strategies.

Brain Behav Immun 2021 03 5;93:368-383. Epub 2020 Nov 5.

Institute of Cancer Stem Cell, Dalian Medical University, 9 West Section, Lvshun South Road, Dalian, Liaoning Province 116044, China; State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-sen University, 651 Dongfeng East Road, Guangzhou, Guangdong Province 510060, China. Electronic address:

Chronic stress is well-known to cause physiological distress that leads to body balance perturbations by altering signaling pathways in the neuroendocrine and sympathetic nervous systems. This increases allostatic load, which is the cost of physiological fluctuations that are required to cope with psychological challenges as well as changes in the physical environment. Recent studies have enriched our knowledge about the role of chronic stress in disease development, especially carcinogenesis. Stress stimulates the hypothalamic-pituitaryadrenal (HPA) axis and the sympathetic nervous system (SNS), resulting in an abnormal release of hormones. These activate signaling pathways that elevate expression of downstream oncogenes. This occurs by activation of specific receptors that promote numerous cancer biological processes, including proliferation, genomic instability, angiogenesis, metastasis, immune evasion and metabolic disorders. Moreover, accumulating evidence has revealed that β-adrenergic receptor (ADRB) antagonists and downstream target inhibitors exhibit remarkable anti-tumor effects. Psychosomatic behavioral interventions (PBI) and traditional Chinese medicine (TCM) also effectively relieve the impact of stress in cancer patients. In this review, we discuss recent advances in the underlying mechanisms that are responsible for stress in promoting malignancies. Collectively, these data provide approaches for NextGen pharmacological therapies, PBI and TCM to reduce the burden of tumorigenesis.
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http://dx.doi.org/10.1016/j.bbi.2020.11.005DOI Listing
March 2021

A Branched Polyelectrolyte Complex Enables Efficient Flame Retardant and Excellent Robustness for Wood/Polymer Composites.

Polymers (Basel) 2020 Oct 22;12(11). Epub 2020 Oct 22.

College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China.

Wood/thermoplastic composites (WPCs) have been restricted in some fields of building construction and electrical equipment because of their inherent high flammability and lower toughness. In this work, a branched crosslinking network polyelectrolyte complex (PEC) has been designed by incorporation of polyethyleneimine (PEI), a cation polyelectrolyte end capped amine groups, into cellulose nanocrystals (CNC), and ammonium polyphosphate (APP) via self-assembling. The hydrogen bonding interactions, penetration, and mechanical interlock provided by PEC effectively enhance the interfacial bonding within matrix, wood fibers, and flame retardant. Interestingly, it generates abundant micropores on the inner structure of WPC. The excellent interfacial bonding performance and easy-to-move molecular chain successfully transfer the stress and induce energy dissipation, simultaneously giving rise to higher strength and toughness for WPC. As well as the PEC endows WPC with a promotion in both smoke suppression and UL-94 V-0 rate. Additionally, the peak heat release rate and total smoke release for WPC obviously reduce by 36.9% and 50.0% respectively in presence of 25% PEC. A simple, eco-friendly, and concise strategy exhibits prospects for fiber-reinforced polymer composites with effective flame retardancy and mechanical robust properties.
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http://dx.doi.org/10.3390/polym12112438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770577PMC
October 2020

Silencing of Long Non-Coding RNA X Inactive Specific Transcript (Xist) Contributes to Suppression of Bronchopulmonary Dysplasia Induced by Hyperoxia in Newborn Mice via microRNA-101-3p and the transforming growth factor-beta 1 (TGF-β1)/Smad3 Axis.

Med Sci Monit 2020 Oct 18;26:e922424. Epub 2020 Oct 18.

Department of Neonatology, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China (mainland).

BACKGROUND Bronchopulmonary dysplasia (BPD) is a chronic lung disease mostly affecting premature infants. Long non-coding RNA (lncRNA) X inactive specific transcript (Xist) is actively involved in pulmonary disease development. The present study explored the potential mechanism of Xist in BPD development. MATERIAL AND METHODS First, newborn BPD mouse models were successfully established. lncRNAs and genes with differential expression were identified using microarray analysis. Various injuries and radial alveolar counts of lung tissues of BPD mice were detected by hematoxylin-eosin staining. Functional assays were utilized to detect alterations of superoxide dismutase (SOD), malondialdehyde (MDA), vascular endothelial growth factor, collagen I, alpha-smooth muscle Actin, TGF-ß1, and Smad3. Then, dual-luciferase reporter gene assay and RNA pull-down assay were performed to clarify the targeting relationship between Xist and miR-101-3p and between miR-101-3p and high-mobility group protein B3 (HMGB3). RESULTS In BPD mice, radial alveolar counts value and SOD activity declined while MDA level increased. Results of microarray analysis found that Xist and HMGB3 were highly expressed in BPD mice. Next, silenced Xist alleviated lung damage in BPD mice. Xist competitively bound to miR-101-3p to activate HMGB3, and overexpressed miR-101-3p mitigated lung damage in BPD mice. Additionally, silenced Xist downregulated the TGF-ß1/Smad3 axis. CONCLUSIONS Our study demonstrated that silencing of Xist suppressed BPD development by binding to miR-101-3p and downregulating HMGB3 and the TGF-b1/Smad3 axis. Our results may provide novel insights for BPD treatment.
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http://dx.doi.org/10.12659/MSM.922424DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580178PMC
October 2020

Utility of Pickering emulsions in improved oral drug delivery.

Drug Discov Today 2020 Sep 17. Epub 2020 Sep 17.

Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai 200443, China; School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery of MOE, Shanghai 201203, China. Electronic address:

Pickering emulsions are surfactant-free emulsions stabilized by solid particles. Their unique structure endows them with good stability, excellent biocompatibility, and environmental friendliness. Pickering emulsions have displayed great potential in oral drug delivery. Several-fold increases in the oral bioavailability or bioaccessibility of poorly soluble drugs, such as curcumin, silybin, puerarin, and rutin, were achieved by using Pickering emulsions, whereas controlled release was found for indomethacin and caffeine. The shell of the interfacial particle stabilizers provides enhanced gastrointestinal stability to the cargos in the oil core. Here, we also discuss general considerations concerning particle stabilizers and design strategies to control lipid digestion.
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http://dx.doi.org/10.1016/j.drudis.2020.09.012DOI Listing
September 2020

Analysis and improvement measures of telephone drug consultation in a specialized hospital of obstetrics and gynaecology in China.

J Clin Pharm Ther 2021 Feb 2;46(1):78-85. Epub 2020 Sep 2.

Department of Pharmacy, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.

What Is Known And Objective: Drug consultation is an important part of pharmaceutical care offered by clinical pharmacists. We explored the characteristics of telephone drug consultations in an obstetrics and gynaecology speciality hospital to provide a reference as to how to improve drug consultation and pharmaceutical care.

Methods: We retrospectively analysed records of telephone consultations regarding medication use between 2014 and 2019 in our hospital. Any consultation cases with incomplete records were excluded from our analysis. Of the 1353 consultation cases included in our study, we further classified them into different categories based on the content of the consultations, the type of medications being inquired about, and the groups of people who sought medication guidance. Pareto analysis was performed to separate the main issues the callers were concerned about from the more minor concerns.

Results And Discussion: The medication issues that prompted the caller to consult with our clinical pharmacists could be divided into 12 categories, among which the main issues concerned usage and dosage, choice of drug variety, drug incompatibility (drug mixture in infusion bag or tube), adjustment of the treatment plan, and skin tests (cumulative percentage 76.3%). The minor issues involved medication use during pregnancy and lactation and adverse reactions. The top three types of drugs that callers asked about were antimicrobials (600 cases, 44.4%), anti-tumour drugs (151 cases, 11.2%) and reproductive system drugs (111 cases, 8.2%). The callers could be divided into four groups as follows: doctors (865 cases, 63.9%), nurses (280 cases, 20.7%), patients (116 cases, 8.6%) and other medical professionals (92 cases, 6.8%).

What Is New And Conclusion: Usage and dosage, choice of drug variety, drug incompatibility, adjustment of treatment plan and skin tests represented the main issues for telephone drug consultations in our hospital. Doctors and nurses were most likely to consult clinical pharmacists regarding these issues. Improved drug consultation services are needed to allow more patients to have access to advice from pharmacists. Such access would better enable pharmacists to fulfil their roles in advising the appropriate use of drugs to ensure better patient care and medication compliance.
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http://dx.doi.org/10.1111/jcpt.13253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818494PMC
February 2021

Preparation and Characterization of Antibacterial Porcine Acellular Dermal Matrices with High Performance.

ACS Omega 2020 Aug 8;5(32):20238-20249. Epub 2020 Aug 8.

College of Biomass Science and Engineering, Sichuan University, No. 24 South Section 1, Yihuan Road, Chengdu, Sichuan 610065, China.

Infection is a common complication in the process of wound management. An ideal wound dressing is supposed to reduce or even prevent the infection while promoting wound healing. A porcine acellular dermal matrix (pADM) has been already used as a wound dressing in clinic due to its capacity to accelerate wound healing. However, not only is pure pADM not antibacterial, its mechanical properties are poor. In this study, an antibacterial pADM with good performance was prepared by adding two natural products as modifiers, quercetin (QCT) and tea tree oil (TTO). The result of Fourier-transform infrared (FTIR) proved that the addition of modifiers did not break the natural triple-helical structure of collagen. Meanwhile, the results of differential scanning calorimetry (DSC), thermogravimetric analysis (TG), mechanic experiment, and enzymatic degradation demonstrated that pADM handled with QCT and TTO (termed QCT-TTO-pADM) had better thermal stability, mechanical strength, and resistance to enzymatic degradation than pADM. Meanwhile, QCT-TTO-pADM had excellent antibacterial activity and showed an antibacterial rate of over 80%. Furthermore, in the cytocompatibility analysis, QCT-TTO-pADM had no side effects on the adhesion, growth, and proliferation of fibroblasts. QCT-TTO-pADM could even accelerate wound healing more efficiently than pADM and glutaraldehyde-modified pADM (GA-pADM). In conclusion, QCT-TTO-pADM was a potential antibacterial wound dressing with good performance.
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http://dx.doi.org/10.1021/acsomega.0c01940DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439368PMC
August 2020

Chronotype and depressive symptoms: A moderated mediation model of sleep quality and resilience in the 1st-year college students.

J Clin Psychol 2021 01 6;77(1):340-355. Epub 2020 Aug 6.

Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education, China; Center for Sleep Research, Center for Studies of Psychological Application, Guangdong Key Laboratory of Mental Health & Cognitive Science, School of Psychology, South China Normal University, Guangzhou, China.

Objective: Depression is highly prevalent among 1st-year college students, and evening chronotype is an important risk factor associated with depression. This study investigates the mediating role of sleep quality and the moderating role of resilience between chronotype and depressive symptoms.

Methods: A total of 4531 students were included in this cross-sectional study. Mediation and moderated mediation models were applied.

Results: The association between chronotype and depressive symptoms was partially mediated by sleep quality, and the direct and indirect effects were moderated by resilience. The negative correlation between chronotype and depressive symptoms was significant in students with low levels of resilience compared with moderate/high levels. The positive correlation between sleep quality and depressive symptoms was strongest in low-level resilience students.

Conclusion: This study reveals that greater eveningness is associated with poorer sleep quality among 1st-year college students, which may lead to severer depression, and highlights the importance of resilience training in reducing depressive symptoms.
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http://dx.doi.org/10.1002/jclp.23037DOI Listing
January 2021

Genomic characterization of MICA gene using multiple next generation sequencing platforms: A validation study.

HLA 2020 10 21;96(4):430-444. Epub 2020 Aug 21.

Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.

We have developed a protocol regarding the genomic characterization of the MICA gene by next generation sequencing (NGS). The amplicon includes the full length of the gene and is about 13 kb. A total of 156 samples were included in the study. Ninety-seven of these samples were previously characterized at MICA by legacy methods (Sanger or sequence specific oligonucleotide) and were used to evaluate the accuracy, precision, specificity, and sensitivity of the assay. An additional 59 DNA samples of unknown ethnicity volunteers from the United States were only genotyped by NGS. Samples were chosen to contain a diverse set of alleles. Our NGS approach included a first round of sequencing on the Illumina MiSeq platform and a second round of sequencing on the MinION platform by Oxford Nanopore Technology (ONT), on selected samples for the purpose of either characterizing new alleles or setting phase among multiple polymorphisms to resolve ambiguities or generate complete sequence for alleles that were only partially reported in the IMGT/HLA database. Complete consensus sequences were generated for every allele sequenced with ONT, extending from the 5' untranslated region (UTR) to the 3' UTR of the MICA gene. Thirty-two MICA sequences were submitted to the IMGT/HLA database including either new alleles or filling up the gaps (exonic, intronic and/or UTRs) of already reported alleles. Some of the challenges associated with the characterization of these samples are discussed.
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http://dx.doi.org/10.1111/tan.13998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589345PMC
October 2020

HEM1 deficiency disrupts mTORC2 and F-actin control in inherited immunodysregulatory disease.

Science 2020 07;369(6500):202-207

Division of Allergy, Immunology, Pulmonary, and Sleep Medicine, Children's Mercy Hospital, Kansas City, MO, USA.

Immunodeficiency often coincides with hyperactive immune disorders such as autoimmunity, lymphoproliferation, or atopy, but this coincidence is rarely understood on a molecular level. We describe five patients from four families with immunodeficiency coupled with atopy, lymphoproliferation, and cytokine overproduction harboring mutations in , which encodes the hematopoietic-specific HEM1 protein. These mutations cause the loss of the HEM1 protein and the WAVE regulatory complex (WRC) or disrupt binding to the WRC regulator, Arf1, thereby impairing actin polymerization, synapse formation, and immune cell migration. Diminished cortical actin networks caused by WRC loss led to uncontrolled cytokine release and immune hyperresponsiveness. HEM1 loss also blocked mechanistic target of rapamycin complex 2 (mTORC2)-dependent AKT phosphorylation, T cell proliferation, and selected effector functions, leading to immunodeficiency. Thus, the evolutionarily conserved HEM1 protein simultaneously regulates filamentous actin (F-actin) and mTORC2 signaling to achieve equipoise in immune responses.
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http://dx.doi.org/10.1126/science.aay5663DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383235PMC
July 2020

3D High-Frequency Ultrasound Imaging of Cartilage-Bone Interface Compared with Micro-CT.

Biomed Res Int 2020 31;2020:6906148. Epub 2020 May 31.

Department of Biomedical Engineering, Faculty of Engineering, Hong Kong Polytechnic University, Kowloon, Hong Kong, China.

Cartilage-bone interface (CBI) is a complex structure which bears important information in pathophysiology of osteoarthritis (OA). While high-frequency ultrasound (US) has been widely used for the investigation of articular cartilage, 3D imaging of CBI using US is less commonly reported in this field. Here, we adopted a 3D high-frequency ultrasound imaging approach specifically for the investigation of CBI in human knee samples. Fifteen osteochondral disks from the tibial plateau of seven OA patients were prepared in vitro and scanned using both high-frequency US and micro-CT imaging. The 3D morphology of the tidemark was reconstructed and compared using an image registration approach between the two imaging modalities. Results showed that the 3D tidemark could be well registered between the two imaging methods with a mean surface discrepancy of 33.2 ± 9.9 m. Quantitative surface waviness/roughness parameter analysis showed significant correlations between the two imaging modalities. An intensity projected en face imaging was proposed to probe characteristic details of the CBI such as its perforations. This study provided evidence for the 3D high-frequency ultrasound as a nonionizing radiation imaging tool potentially useful to evaluate the change of CBI in basic research of join diseases including OA.
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http://dx.doi.org/10.1155/2020/6906148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285412PMC
March 2021

Improving performance of molecularly imprinted polymers prepared with template of low purity utilizing the strategy of macromolecular crowding.

J Chromatogr A 2020 Aug 12;1624:461155. Epub 2020 May 12.

Xinjiang Technical Institute of Physics and Chemistry, State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

The topic in the present paper is to prepare molecularly imprinted polymer (MIP) using the template molecule with low purity. For the first time, a surrounding of macromolecular crowding was established to promote the formation of the complex of the template with functional monomer efficiently thus highly pure template molecule was unnecessary. In this study, the MIP monolith was synthesized using low purity lactucopicrin as template in place of highly pure one, and polystyrene was used as macromolecular crowding agent. 4-Vinylpyridine and ethyleneglycol dimethacrylate were used as functional monomer and crosslinker, respectively. Polymerization parameters, including the ratio of functional monomer/template, various template concentrations, and PS concentration on the affinity of the resulting MIP were systematically investigated. For the lactucopicrin MIP made with the purity of lactucopicrin of 92%, the imprinting factor can be up to 2.2. The resulting MIP was filled in solid phase extraction (SPE) cartridge to purify lactucopicrin from the crude extract of Cichorium glandulosum Boiss. et Huet. After two cycles of MIP SPE for the crude extract, the highest recovery and purity of lactucopicrin was 64.8% and 97.8%, respectively. The results indicated that the use of macromolecular crowding agent is an effective method for improving the performance of the MIP prepared with the template of low purity, particularly valuable to the cases in which the highly pure target molecule is hard to be obtained.
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http://dx.doi.org/10.1016/j.chroma.2020.461155DOI Listing
August 2020

Cross-interface transfer of polycyclic aromatic hydrocarbons (PAHs) in a shallow urban lake in Shanghai, China based on the fugacity model.

Sci Total Environ 2020 Sep 12;736:139369. Epub 2020 May 12.

Key Laboratory of Geographic Information Science (Ministry of Education), East China Normal University, 200241 Shanghai, China; School of Geographical Sciences, East China Normal University, 200241 Shanghai, China.

Shallow urban lakes are important urban ecosystems; however, these systems are subject to severe polycyclic aromatic hydrocarbons (PAHs) contamination. An understanding of the distribution and dynamics of PAHs in lakes is required to restore the functions of lake ecosystems and to ensure the ecological security of urban water sources. The Quantitative Water Air Sediment Interaction (QWASI) model and partition coefficient and fugacity fraction methods were applied to estimate the multimedia transfers of PAHs in Dianshan Lake, a typical shallow lake in Shanghai, China. In addition, some new concepts and methods related to PAH transfers were introduced. The results showed that while the gas-solid partition in the area remained in non-equilibrium, the influence of pollution sources tended to weaken. Atmospheric advection was the main source of PAHs to the lake, and a portion of the net loss of advection was transformed into total flux of cross-interface transfers, in which transport fluxes from air to water and from water to sediment were dominant, with a significant correlation between the two types of transfer. The large resuspension of high molecular weight (HMW)-PAHs occurred, possibly related to frequent hydrodynamic disturbances. Furthermore, this study explored the distribution of PAHs among different compartments and the seasonal variation of multimedia transfers. Sensitivity analysis showed that the model is remarkably sensitive to four parameters including temperature and advection. Monte Carlo uncertainty analysis verified that the simulation results were stable and reliable. The results can provide a theoretical basis for the monitoring and control of shallow lake pollution.
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http://dx.doi.org/10.1016/j.scitotenv.2020.139369DOI Listing
September 2020

Self-Collection for Cervical Screening Programs: From Research to Reality.

Cancers (Basel) 2020 Apr 24;12(4). Epub 2020 Apr 24.

VCS Foundation, Carlton South, VIC 3053, Australia.

In 2018, there were an estimated 570,000 new cases of cervical cancer globally, with most of them occurring in women who either had no access to cervical screening, or had not participated in screening in regions where programs are available. Where programs are in place, a major barrier for women across many cultures has been the requirement to undergo a speculum examination. With the emergence of HPV-based primary screening, the option of self-collection (where the woman takes the sample from the vagina herself) may overcome this barrier, given that such samples when tested using a PCR-based HPV assay have similar sensitivity for the detection of cervical pre-cancers as practitioner-collected cervical specimens. Other advantages of HPV-based screening using self-collection, beyond the increase in acceptability to women, include scalability, efficiency, and high negative predictive value, allowing for long intervals between negative tests. Self-collection will be a key strategy for the successful scale up of cervical screening programs globally in response to the WHO call for all countries to work towards the elimination of cervical cancer as a public health problem. This review will examine self-collection for HPV-based cervical screening including the collection devices, assays and possible routine laboratory processes considering how they can be utilized in cervical screening programs.
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http://dx.doi.org/10.3390/cancers12041053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226191PMC
April 2020

Complex Linkage Disequilibrium Effects in HLA-DPB1 Expression and Molecular Mismatch Analyses of Transplantation Outcomes.

Transplantation 2021 03;105(3):637-647

Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA.

Background: HLA molecular mismatch (MM) is a risk factor for de novo donor-specific antibody (dnDSA) development in solid organ transplantation. HLA expression differences have also been associated with adverse outcomes in hematopoietic cell transplantation. We sought to study both MM and expression in assessing dnDSA risk.

Methods: One hundred three HLA-DP-mismatched solid organ transplantation pairs were retrospectively analyzed. MM was computed using amino acids (aa), eplets, and, supplementarily, Grantham/Epstein scores. DPB1 alleles were classified as rs9277534-A (low-expression) or rs9277534-G (high-expression) linked. To determine the associations between risk factors and dnDSA, logistic regression, linkage disequilibrium (LD), and population-based analyses were performed.

Results: A high-risk AA:GX (recipient:donor) expression combination (X = A or G) demonstrated strong association with HLA-DP dnDSA (P = 0.001). MM was also associated with HLA-DP dnDSA when evaluated by itself (eplet P = 0.007, aa P = 0.003, Grantham P = 0.005, Epstein P = 0.004). When attempting to determine the relative individual effects of the risk factors in multivariable analysis, only AA:GX expression status retained a strong association (relative risk = 18.6, P = 0.007 with eplet; relative risk = 15.8, P = 0.02 with aa), while MM was no longer significant (eplet P = 0.56, aa P = 0.51). Importantly, these risk factors are correlated, due to LD between the expression-tagging single-nucleotide polymorphism and polymorphisms along HLA-DPB1.

Conclusions: The MM and expression risk factors each appear to be strong predictors of HLA-DP dnDSA and to possess clinical utility; however, these two risk factors are closely correlated. These metrics may represent distinct ways of characterizing a common overlapping dnDSA risk profile, but they are not independent. Further, we demonstrate the importance and detailed implications of LD effects in dnDSA risk assessment and possibly transplantation overall.
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http://dx.doi.org/10.1097/TP.0000000000003272DOI Listing
March 2021

Effects of Cyclopamine on the Viability of Articular Chondrocytes in Rats with Adjuvant Arthritis .

Ann Clin Lab Sci 2020 Jan;50(1):85-91

Microscopic Morphological Center Laboratory, Anhui Medical University, China

The aim of the present study is to investigate the effect of cyclopamine, a hedgehog signaling pathway inhibitor, on adjuvant arthritis (AA), rat articular chondrocyte viability, and part mechanisms In this study, an AA rat model was established by Freund's complete adjuvant (FCA). The arthritis index (AI), secondary paw swelling degree, and HE staining were used to evaluate whether the model was successfully established. Chondrocytes of the ankle joint of AA rats were cultured and identified. Cyclopamine (0, 0.03, 0.1, 0.3, 1, 3, 10 and 30 mg/l) was administered to determine chondrocyte viability. Chondrocyte apoptosis was detected by Annexin V-FITC/PI double dye. The expression of hedgehog signaling pathway-related proteins Shh, Ptch1, and Gli1 in chondrocytes was detected by western blotting. The results show that AA was successfully induced by FCA since the AI of AA rats and secondary paw swelling degree increased and the cartilage tissue of the rats' ankle joint was damaged. Thus, the chondrocytes were successfully cultured following the identification of toluidine blue and type II collagen. Cyclopamine (0.03, 0.1, 0.3, 1, 3, 10 and 30 mg/l) could increase the viability of chondrocytes and reduce the apoptotic rate of chondrocytes. As compared with the control group, different doses of cyclopamine (0.3, 3 and 10 mg/l) significantly decreased the expression of Shh, Ptch1 and Gli1 proteins in AA chondrocytes. Therefore, an AA rat model was successfully established in the present study and cyclopamine improved the viability and inhibited the apoptosis of chondrocytes. This is an effect that may be associated with the inhibition of the chondrocyte hedgehog signaling pathway.
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January 2020

Integrated microfluidic chip for on-line proteome analysis with combination of denaturing and rapid digestion of protein.

Anal Chim Acta 2020 Mar 14;1102:1-10. Epub 2020 Jan 14.

Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China. Electronic address:

A microfluidic platform based on the integration of denaturation and online immobilized enzyme reactor (IMER) digestion for protein pretreatment was first developed on a glass chip. The design of three inlet channels and two groups of snake channel in glass chip can allow the protein solution, the reducing reagent and the alkylating agent to be simultaneously injected into the chip channel and ensured the reaction solution on-line efficient mixing and sufficient reacting. By thiol-ene click chemistry, the capillary-based and glass chip-based trypsin IMER on the surface of poly(trimethylolpropane trimethacrylate) monolith were fabricated. The wide range of flow rate tolerance (0.8-5.0 μL/min), and the acceptable reproducibility (RSD% = 3.1%, n = 5) and stability (13.8% decrease of enzyme activity in 2 months) indicated the feasibility of using IMER for online digestion of proteins. Compared with the solution denaturation-offline IMER digestion, the integrated microfluidic platform of chip denaturation-chip IMER and chip denaturation-online IMER have comparable protein identification ability for mouse liver protein with a similar number of protein (798 or 826 vs. 843) and unique peptides (3923 or 4593 vs. 3916). More importantly, the easy and fast digestion of protein samples and possible combination with MS revealed that this microfluidic platform can be a potential method for rapid proteomics analysis.
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http://dx.doi.org/10.1016/j.aca.2020.01.025DOI Listing
March 2020

Hydrogen-bond enhancement triggered structural evolution and band gap engineering of hybrid perovskite (CHCHNH)PbI under high pressure.

Phys Chem Chem Phys 2020 Jan 16;22(4):1841-1846. Epub 2020 Jan 16.

State Key Laboratory of Superhard Materials, College of Physics, Jilin University, Changchun 130012, China.

Hybrid organic-inorganic perovskites (HOIPs) have gained substantial attention due to their excellent photovoltaic and optoelectronic properties. Herein, we comprehensively investigate a typical two-dimensional (2D) hybrid perovskite (CHCHNH)PbI to track its structural and band gap evolution applied by the maximum pressure of 27.2 GPa. Remarkably, an unprecedented band gap narrowing down to the Shockley-Queisser limit is observed upon compression to 20.1 GPa. Two phase transitions have been observed during this process: the ambient Pbca phase converts into the Pccn phase at 4.6 GPa and then undergoes an isostructural phase transition at 7.7 GPa. The Fourier Transform Infrared (FTIR) spectroscopy reveals that pressure-enhanced hydrogen bonding plays an important role in structural modifications and band gap variations. This work not only enables high pressure as a clean tool to tune the structure and band gap of hybrid perovskite, but also maps a pioneering route towards realizing ideal photovoltaic materials-by-design.
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http://dx.doi.org/10.1039/c9cp05904kDOI Listing
January 2020

Reinforcement of Polycaprolactone/Chitosan with Nanoclay and Controlled Release of Curcumin for Wound Dressing.

ACS Omega 2019 Dec 19;4(27):22292-22301. Epub 2019 Dec 19.

College of Biomass Science and Engineering, Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, Research Center of Biomedical Engineering, and College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.

A novel clay-reinforced polycaprolactone/chitosan/curcumin (PCl/CS/Clay/Cur) composite film was fabricated in this study. The prepared Cur-loading composite films were characterized with attenuated total reflection Fourier transformed infrared spectroscopy, scanning electron microscopy, atomic force microscopy, water contact angle, differential scanning calorimetry, thermogravimetric analysis, and X-ray diffraction, and the results showed good dispersion of clay in the composite films. The addition of nanoclay was found to significantly increase the tensile strength. Also, the clay-enhanced drug-loading films exhibited better controlled-release profiles of Cur than those membranes without clay. Skin disinfection test demonstrated that the curcumin-loaded film could protect wound from bacterial infection. Cytotoxicity analysis proved the good biocompatibility of the composite films. The clay-enhanced Cur-loading films might be promising candidates for wound care.
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http://dx.doi.org/10.1021/acsomega.9b02217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941175PMC
December 2019

Superconducting Zirconium Polyhydrides at Moderate Pressures.

J Phys Chem Lett 2020 Feb 10;11(3):646-651. Epub 2020 Jan 10.

State Key Laboratory of Superhard Materials, College of Physics , Jilin University , Changchun 130012 , China.

Highly compressed hydrides have been at the forefront of the search for high- superconductivity. The recent discovery of record-high 's in HS and LaH under high pressure fuels the enthusiasm for finding good superconductors in similar hydride groups. Guided by first-principles structure prediction, we successfully synthesized ZrH and ZrH at modest pressures (30-50 GPa) in diamond anvil cells by two different reaction routes: ZrH + H at room temperature and Zr + H at ∼1500 K by laser heating. From the synchrotron X-ray diffraction patterns, ZrH is found to have a 3̅ structure corresponding to the familiar A15 structure, and ZrH has an 4̅3 structure isostructural to ThH. Electrical resistance measurement and the dependence of on the applied magnetic field of the sample showed the emergence of two superconducting transitions at 6.4 and 4.0 K at 40 GPa, which correspond to the two 's for ZrH and ZrH.
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http://dx.doi.org/10.1021/acs.jpclett.9b03632DOI Listing
February 2020

The efficacy and molecular mechanism of the effect of schisandrin b on the treatment of erectile dysfunction.

Iran J Basic Med Sci 2019 Aug;22(8):866-871

Department of Urology and Andrology, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200001, People's Republic of China.

Objectives: The purpose of this study is to determine the efficacy and molecular mechanism of the effect of schisandrin b (SCHB) on treating erectile dysfunction (ED) in a rat model with bilateral cavernous crushing nerve injury.

Materials And Methods: The ED rat model was established with bilateral cavernous nerve crushing, and then confirmed by apomorphine. Fifty healthy eight-week-old ED rats were randomly assigned into five group, including control group (sham surgery), bilateral cavernous nerve crushing injury group (BCNC), BCNC with low SCHB (100 mg/d), BCNC with medium SCHB (200 mg/d) and BCNC with high SCHB (400 mg/d). For the last three groups, SCHB was given for 2 months. Then, we examined intracavernosal pressure (ICP), cyclic nucleotides (cAMP, cGMP), endothelial nitric oxide synthase (eNOS) and neuronal NOS (nNOS) in all groups.

Results: In the study of ICP, SCHB was able to improve ED in a dose-dependent manner. In addition, as compared to the BCNC group, the relative expression of eNOS and nNOS in medium and high concentration of SCHB-treated groups are higher than BCNC group. Moreover, all groups treated with SCHB showed a significant higher expression level of cAMP and cGMP.

Conclusion: These results suggested that SCHB were able to significantly improve the ED on rat model through the NO-cGMP and cAMP- protein kinase A (PKA) pathway.
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http://dx.doi.org/10.22038/ijbms.2019.27455.6701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6760479PMC
August 2019

Design and Implementation of Polymerase Chain Reaction Device for Aptamers Selection of Tumor Cells.

J Nanosci Nanotechnol 2020 03;20(3):1332-1340

Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China.

Nucleic acid aptamers are a kind of one-dimensional biological nanomaterials and have found many applications. This paper designed and implemented a polymerase chain reaction (PCR) amplification device with a reaction volume of 500 L, which can be used for the amplification of nucleic acid aptamers of tumor cells in the aptamer selection. This device mainly includes a control module, a temperature measuring module, a PCR amplification tube, a metal tank module, a liquid crystal display (LCD) and operation module and a cooling module. The new PCR amplification chamber is matched with the designed metal tank to ensure the temperature uniformity of the PCR amplification solution. The control module based on the STM32F103RCT6 manages the workflow of the entire device. The PCR amplification chamber and PT100 sensors on the metal tank formed a closed-loop feedback system, and the incremental proportional-integral-derivative (PID) algorithm was used to achieve the precise temperature control. In addition, we introduced the Smith predictive compensation algorithm to solve the temperature hysteresis problem of the PCR amplification chamber. The experimental results showed that the PCR device can meet the requirements for the nucleic acid aptamer selection of tumor cells. The device can also be used in other experiments with large-volume PCR amplification.
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http://dx.doi.org/10.1166/jnn.2020.17356DOI Listing
March 2020

Design and Implementation of a High-Throughput Vibrating Module for Nucleic Acid Detection System.

J Nanosci Nanotechnol 2020 04;20(4):2165-2170

Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China.

The high-throughput nucleic acid detection system provides a good solution for detecting nucleic acids more safely, rapidly and accurately, which greatly improves the detection efficiency. Highthroughput nucleic acid detection mainly includes three steps: signal acquisition, signal amplification and signal processing. Therefore, obtaining the purified nucleic acid is the primary task of the nucleic acid detection, and the quality of the nucleic acid has a significant impact on results. In this paper, we employed the magnetic nanoparticle technology for extracting nucleic acids based on the platform of large liquid handling workstation and designed a matching vibrating module. The involved steps of core method, magnetic bead nucleic acid extraction technology, are mainly concerned with the cell lysis, nucleic acid binding, nucleic acid purification and magnetic particles elution. During the extraction process, specific temperature is required for the lysis and elution. It was shown that the temperature control part designed in this paper has the reliable stability, high accuracy by using the incremental proportion-integration-differentiation (PID) algorithm, with the control accuracy up to ±0.5 °C. The temperature regulating time is about 90 s, which can meet the experimental requirements. Besides, the vibrating uniformity of this module was further verified by protein concentration test, which proved that the module has the excellent performance and can be consistent with the experimental indictors of the nucleic acid extraction.
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http://dx.doi.org/10.1166/jnn.2020.17328DOI Listing
April 2020
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