Publications by authors named "Yanni Tang"

4 Publications

  • Page 1 of 1

Soil applied Ca, Mg and B altered phyllosphere and rhizosphere bacterial microbiome and reduced Huanglongbing incidence in Gannan Navel Orange.

Sci Total Environ 2021 May 26;791:148046. Epub 2021 May 26.

College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China; Hubei Provincial Engineering Laboratory for New-Type Fertilizer, Wuhan 430070, China; Research Center of Trace Elements, Wuhan 430070, China; Hubei Key Laboratory of Soil Environment and Pollution Remediation, Wuhan 430070, China. Electronic address:

Huanglongbing (HLB) caused by 'Ca. Liberibacter. Asiaticus (Clas)' is one of the destructive diseases for citrus, threatening the development of citrus industry. Adopting a proper fertilization method instead of using pesticides seems particularly important, which would contribute to a sustainable development of orchard. In this study, the impact of soil application of nutrients combined with foliar spray of macro- and micro-nutrients on the incidence of HLB and the phyllosphere and rhizosphere bacterial microbiome was investigated in Gannan Navel Orange orchard from 2015 to 2018. Compared with the control (T1), the yield of Gannan Navel Orange in all other treatments applied with macro- and micro-nutrients increased significantly in 2018 (by 20.5%-45.8%), but not in the first two years (2016-17). Among treatments, Ca + Mg + B application in soil (T2) showed the highest yield and lowest HLB incidence. According to the PCR results, CLas was negative in T2 but positive in the control, which directly proved HLB incidence was reduced with Ca + Mg + B application in soil. Moreover, 16S rRNA sequencing was used to characterize rhizosphere and phyllosphere microbial communities. Results showed that microbial biodiversity was increased and microbial community structure was altered in T2 treatment, of which the beneficial bacteria were enriched in phyllosphere and rhizosphere. The results of PICRUSt showed that in T2 treatment, rhizosphere microbe contained more membrane transport (ABC transporters) genes, while, carbohydrate metabolism genes were enriched in the control rhizosphere due to HLB obstruct the photosynthetic metabolite transport. In summary, results indicated that macro- and micro-nutrients application improved the yield of Gannan Navel Orange and soil application of Ca + Mg + B reduced HLB incidence by altering microbial community structure and increasing microbial biodiversity. This study developed an environment-friendly way to reduce HLB incidence and improve the yield of citrus.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148046DOI Listing
May 2021

Enhancement and improvement of selenium in soil to the resistance of rape stem against Sclerotinia sclerotiorum and the inhibition of dissolved organic matter derived from rape straw on mycelium.

Environ Pollut 2020 Oct 19;265(Pt A):114827. Epub 2020 May 19.

College of Resources and Environment, Huazhong Agricultural University, Hubei Provincial Engineering Laboratory for New-Type Fertilizer, Research Center of Trace Elements, Hubei Key Laboratory of Soil Environment and Pollution Remediation, Wuhan, 430070, China; Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, Wuhan, 430070, China. Electronic address:

Sclerotinia stem rot (SSR), caused by Sclerotinia sclerotiorum (S. sclerotiorum), one of the most destructive diseases in many crops including Brassica napus L. The extensive use of fungicides to control S. sclerotiorum caused severe damage to the environment in the long term. Increasing study reported that selenium (Se) is a beneficial element for plant by promoting growth and enhancing disease resistance. In this study, it was found that Se in soil shortened lesion length by 19.14% on rape stem infected with S. sclerotiorum. While resistance mechanism of rape stem against S. sclerotiorum remains unknown. Transcriptomic analysis of rape stem was performed and the results indicated that genes related to antifungal pathways were up-regulated. Moreover, metabonomic analysis was carried out to study the inhibitive effect of the dissolved organic matter derived from rape straw with Se pretreatment in soil (RSDOM) on S. sclerotiorum mycelium, results showed that RSDOM caused severe damage to energy metabolism of mycelium. Further study indicated that RSDOM decreased the pathogenicity of mycelium on rape leaves significantly, and enhanced content of chlorophyII, carotenoids, OD phenol and activities of phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO) in rape leaves, which suggested that RSDOM plays a positive role in regulating oxidative stress responses of plant when infected with S. sclerotiorum. In addition, when compared with dimcthachlon (DIM) treatment alone, DIM combined with RSDOM resulted in higher inhibition on mycelial growth of S. sclerotiorum (the inhibition ratio of nearly 60%). Results in this study suggested that Se enhanced the resistance of rape stem against S. sclerotiorum because of the up-regulated genes related to antifungal pathways, and RSDOM improved the mycelial growth inhibition and decreased the pathogenicity of mycelium on rape leaves. Overall, Se as well as Se-enrich byproducts, possessed great potential to be developed as ecological fungicides for controlling S. sclerotiorum.
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http://dx.doi.org/10.1016/j.envpol.2020.114827DOI Listing
October 2020

Isolation and characterization of Populus xyloglucan endotransglycosylase/hydrolase (XTH) involved in osmotic stress responses.

Int J Biol Macromol 2020 Jul 12;155:1277-1287. Epub 2019 Nov 12.

College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P.R. China; The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, Beijing 100083, P.R. China. Electronic address:

Xyloglucan endotransglycosylase/hydrolase (XTH) belongs to the GH16 subfamily of the glycoside hydrolases of carbohydrate active enzymes and plays an important role in the structure and function of plant cell walls. In this study, 11 members of the XTH gene family were cloned from Populus tomentosa. A bioinformatics analysis revealed that 11 PtoXTHs could be classified into three groups, where PtoXTH27 and PtoXTH34 were most likely to exhibit XTH activity. Biochemical analyses of purified PtoXTHs demonstrated that PtoXTH27 and PtoXTH34 had detectable xyloglucan endotransglucosylase (XET) activity, while the others did not exhibit XET or XEH activity. Moreover, enzymatic assays revealed that the optimum reaction temperature of both PtoXTH27 and PtoXTH34 was 37 °C, while their optimum pH values differed, such that PtoXTH27 was 6.0 and PtoXTH34 was 5.0. Enzyme kinetic parameters indicated that PtoXTH34 had higher affinity for the receptor substrate, XXXG, implying that PtoXTH34 and PtoXTH27 in plants have different substrate structure specificity. Finally, heterologous expression of XTH significantly increased intracellular total sugar content and osmotolerance of yeast cells, indicating that PtoXTH27 and PtoXTH34 are potentially involved in osmotic stress responses. These results clearly demonstrate the enzymatic characteristics and putative role of XTH in osmotic stress responses.
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http://dx.doi.org/10.1016/j.ijbiomac.2019.11.099DOI Listing
July 2020

Selenium reduces the pathogenicity of Sclerotinia sclerotiorum by inhibiting sclerotial formation and germination.

Ecotoxicol Environ Saf 2019 Nov 5;183:109503. Epub 2019 Aug 5.

College of Resources and Environment, Huazhong Agricultural University / Hubei Provincial Engineering Laboratory for New-Type Fertilizer / Research Center of Trace Elements / Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, Wuhan 430070, China. Electronic address:

Sclerotinia sclerotiorum (S. sclerotiorum) is a devastating fungal pathogen with worldwide distribution, and threatened the agro-ecological safety in the long term. To control the damage caused by Sclerotinia diseases, as well as consider the fungicide resistance and chemical residues, strategy of which plant nutritional regulation, as an eco-friendly approach, is gaining much significance. Selenium (Se), as a beneficial microelement for plant, has been manifested to be effective in inhibiting the mycelial growth of S.sclerotiorum in our previous study. In the present study, we observed that Se (both selenate and selenite) inhibited the formation of sclerotia, which is an important life form in the disease cycle of S. sclerotiorum. And the inhibition ratios of number of sclerotia in treatments of Se(VI) and Se(IV) were 54.55% and 43.84%, respectively; the inhibition ratios of weight of sclerotia in treatments of Se(VI) and Se(IV) were 42.29% and 25.67%, respectively. Results suggested that Se inhibited mycelial growth, severely damaged sclerotial ultrastructure, reduced the capacity of acid production, decreased superoxide dismutase (SOD) and catalase (CAT) activities, increased the content of hydrogen peroxide (HO) and superoxide anion (O) in mycelium, and all of these resulted in the reduction in sclerotial formation. Further studies revealed that Se application in medium increased Se concentration in sclerotia and thus inhibited sclerotial germination. Moreover, the pathogenicity of mycelia germinating from sclerotia that pretreated with Se, decreased significantly to rape leaves. These findings broadened our understanding of Se application in plant protection, as well as provided evidences for developing environment-friendly fungicide for S. sclerotiorum control.
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http://dx.doi.org/10.1016/j.ecoenv.2019.109503DOI Listing
November 2019