Publications by authors named "Yanmei Wang"

194 Publications

Image Features of Resting-State Functional Magnetic Resonance Imaging in Evaluating Poor Emotion and Sleep Quality in Patients with Chronic Pain under Artificial Intelligence Algorithm.

Contrast Media Mol Imaging 2022 4;2022:5002754. Epub 2022 Jan 4.

Department of Otorhinolaryngology, Weifang People's Hospital Weifang Shandong, Weifang 261041, Shandong, China.

The balanced iterative reducing and clustering using hierarchies (BIRCH) method was adopted to optimize the results of the resting-state functional magnetic resonance imaging (RS-fMRI) to analyze the changes in the brain function of patients with chronic pain accompanied by poor emotion or abnormal sleep quality in this study, so as to provide data support for the prevention and treatment of clinical chronic pain with poor emotion or sleep quality. 159 patients with chronic pain who visited the hospital were selected as the research objects, and they were grouped according to the presence or absence of abnormalities in emotion and sleep. The patients without poor emotion and sleep quality were set as the control group (60 cases), and the patients with the above symptoms were defined in the observation group (90 cases). The brain function was detected by RS-fMRI technology based on the BIRCH algorithm. The results showed that the rand index (RI), adjustment of RI (ARI), and Fowlkes-Mallows index (FMI) results in the k-means, flow cytometry (FCM), and BIRCH algorithms were 0.82, 0.71, and 0.88, respectively. The scores of Hamilton Depression Scale (HAHD), Hamilton Anxiety Scale (HAMA), and Pittsburgh Sleep Quality Index (PSQI) were 7.26 ± 3.95, 7.94 ± 3.15, and 8.03 ± 4.67 in the observation group and 4.03 ± 1.95, 5.13 ± 2.35, and 4.43 ± 2.07 in the control group; the higher proportion of RS-fMRI was with abnormal brain signal connections. A score of 7 or more meant that the number of brain abnormalities was more than 90% and that of less than 7 was less than 40%, showing a statistically obvious difference in contrast ( < 0.05). Therefore, the BIRCH clustering algorithm showed reliable value in the optimization of RS-fMRI images, and RS-fMRI showed high application value in evaluating the emotion and sleep quality of patients with chronic pain.
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http://dx.doi.org/10.1155/2022/5002754DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8752300PMC
January 2022

Corrigendum to "Epstein-Barr virus (EBV) hyperimmune globulin isolated from donors with high gp350 antibody titers protect humanized mice from challenge with EBV" [Virology 561 (2021) 80-86].

Virology 2022 Jan 20;568:12. Epub 2022 Jan 20.

Medical Virology Section, Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, MD, 20892, USA. Electronic address:

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http://dx.doi.org/10.1016/j.virol.2022.01.007DOI Listing
January 2022

Quality of work life and work engagement among nurses with standardised training: The mediating role of burnout and career identity.

Nurse Educ Pract 2022 Jan 10;58:103276. Epub 2021 Dec 10.

Shengjing Hospital of China Medical University-Nursing Department, China. Electronic address:

Aim: To explore the impact of the quality of work life of nurses with standardised training on their work engagement and its impact path, that is, the intermediary role of career identity and job burnout.

Background: Most countries currently face the serious problem of a shortage of nurses and this difficulty is likely to persist for a long time. In recent years, some hospitals in China have attempted to try out clinical standardised training for new nurses for a duration of at least two years. They need to cope with clinical work, training assessments, and low salaries. Ways to improve the work input of new nurses play a pivotal role in reducing the turnover rate of nurses.

Design: The current research is a cross-sectional study.

Methods: Valid questionnaires were received from 245 nurses from the tertiary hospitals between June and July 2021. AMOS was used to conduct a structural equation model to evaluate the mediating effect of burnout and career identity on the association between the quality of work life and work engagement. We describe the study in accordance with the STROBE statement.

Results: The quality of the work life of nurses with standardised training is at a medium to high level (191.62 ± 27.18) and their work engagement is at a relatively high level (75.77 ± 19.60). Quality of work life was negatively correlated with burnout (r = -0.672, p < 0.001) and positively correlated with career identity (r = 0.810, p < 0.001) and work engagement (r = 0.642, p < 0.001). Moreover, a positive relationship was found between the quality of work life and work engagement (β =0.644), which was mediated by burnout and career identity (β =0.144, β =0.218).

Conclusions: Although the work engagement of nurses with standardised training is encouraging, that work engagement decreases with the increase in the age of nurses, still needs investigation. When nurse managers want to improve the work engagement of new nurses, they should not only pay attention to the quality of work life but take career identity and burnout into consideration.
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http://dx.doi.org/10.1016/j.nepr.2021.103276DOI Listing
January 2022

Interpersonal neural synchronization could predict the outcome of mate choice.

Neuropsychologia 2022 Jan 7;165:108112. Epub 2021 Dec 7.

Shanghai Key Laboratory of Mental Health and Psychological Crisis Intervention, Affiliated Mental Health Center (ECNU), School of Psychology and Cognitive Science, East China Normal University, Shanghai, 200062, China; School of Psychological and Cognitive Sciences, Peking University, Beijing, 100871, China. Electronic address:

Although mate choice is crucial for adults, its neural basis remains elusive. In the current study, we combined the functional near-infrared spectroscopy (fNIRS)-based hyperscanning and speed-dating to investigate the inter-brain mechanism of mate choice. Each participant was paired with two opposite-sex partners (participants) in separate speed-dating sessions and was asked to decide whether to engage in a further relationship with the paired partner after each session. The physical attraction of the daters was rated by their partners at the beginning of the dating whereas the social attraction was rated after the dating. Interpersonal neural synchronization (INS) at the dorsolateral prefrontal cortex during speed-dating rather than reading task predicts the outcome of mate choice. Moreover, social attraction rather than physical attraction affects INS during speed-dating. These findings demonstrate for the first time that INS predicts the outcome of mate choice of interacting daters in ecologically valid settings during their initial romantic encounter.
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http://dx.doi.org/10.1016/j.neuropsychologia.2021.108112DOI Listing
January 2022

Self-Healing Mechanism and Conductivity of the Hydrogel Flexible Sensors: A Review.

Gels 2021 Nov 16;7(4). Epub 2021 Nov 16.

Industry Engineering Department, School of Mechanical Engineering, Northwestern Polytechnical University, Xi'an 710072, China.

Sensors are devices that can capture changes in environmental parameters and convert them into electrical signals to output, which are widely used in all aspects of life. Flexible sensors, sensors made of flexible materials, not only overcome the limitations of the environment on detection devices but also expand the application of sensors in human health and biomedicine. Conductivity and flexibility are the most important parameters for flexible sensors, and hydrogels are currently considered to be an ideal matrix material due to their excellent flexibility and biocompatibility. In particular, compared with flexible sensors based on elastomers with a high modulus, the hydrogel sensor has better stretchability and can be tightly attached to the surface of objects. However, for hydrogel sensors, a poor mechanical lifetime is always an issue. To address this challenge, a self-healing hydrogel has been proposed. Currently, a large number of studies on the self-healing property have been performed, and numerous exciting results have been obtained, but there are few detailed reviews focusing on the self-healing mechanism and conductivity of hydrogel flexible sensors. This paper presents an overview of self-healing hydrogel flexible sensors, focusing on their self-healing mechanism and conductivity. Moreover, the advantages and disadvantages of different types of sensors have been summarized and discussed. Finally, the key issues and challenges for self-healing flexible sensors are also identified and discussed along with recommendations for the future.
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http://dx.doi.org/10.3390/gels7040216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8628684PMC
November 2021

Identification and functional verification of the target protein of pedunsaponin A in the gills of Pomacea canaliculata.

Pest Manag Sci 2021 Nov 2. Epub 2021 Nov 2.

College of Agronomy, Sichuan Agricultural University, Chengdu, China.

Background: Based on previous research indicating that pedunsaponin A (PA) can destroy the gills of Pomacea canaliculata, we chose the gill as the main research object, and identified the target protein of PA in the gills of P. canaliculata through proteomics and RNA interference (RNAi).

Results: Proteomics showed that 180 proteins were downregulated after PA treatment in P. canaliculata. Among them, we chose advillin (PcAdv), receptor type tyrosine protein phosphatase (PcRT) and unconventional myosin heavy chain 6 (PcUM) as candidate target proteins through bioinformatics analysis. The small interfering RNA (siRNA) with the best interference effect was identified through further screening. Gene interference rates were 97%, 98% and 82% for PcAdv, PcRT and PcUM, respectively. The results showed that after RNAi treatment, the mortality of P. canaliculata treated with PcAdv (60.0%) was significantly lower than that for the control (93.3%); histological analysis showed that the structure of the gill was intact, cilia shedding was reduced, and the survival rate of hemocytes had increased.

Conclusion: These findings indicate that, when the protein was absent or suppressed, the channel for entry of PA into the hemocytes of P. canaliculata was blocked, which reduced PA binding to hemocytes, and that there is a close relationship between shedding of gill cilia and PA entry into hemocytes. PcAdv is thus the key protein in PA destruction of gill cilia. Locating the proteins in gills that interact with drugs and investigating their mode of action is of great importance in the development of new molluscicides to control P. canaliculata populations.
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http://dx.doi.org/10.1002/ps.6704DOI Listing
November 2021

Subtypes of nurses' mental workload and interaction patterns with fatigue and work engagement during coronavirus disease 2019 (COVID-19) outbreak: A latent class analysis.

BMC Nurs 2021 Oct 22;20(1):206. Epub 2021 Oct 22.

Department of Nursing, Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of TCM, 274 Middle Zhi Jiang Road, Shanghai, 200071, China.

Background: Nurses play critical roles when providing health care in high-risk situations, such as during the COVID-19 outbreak. However, no previous study had systematically assessed nurses' mental workloads and its interaction patterns with fatigue, work engagement and COVID-19 exposure risk.

Methods: A cross-sectional study was conducted via online questionnaire. The NASA Task Load Index, Fatigue Scale-14, and Utrecht Work Engagement Scale were used to assess nurses' mental workload, fatigue and work engagement, respectively. A total of 1337 valid questionnaires were received and analyzed. Nurses were categorized into different subgroups of mental workload via latent class analysis (LCA). Cross-sectional comparisons, analysis of covariance (ANCOVA), and multivariate (or logistic) regression were subsequently performed to examine how demographic variables, fatigue and work engagement differ among nurses belonging to different subgroups.

Results: Three latent classes were identified based on the responses to mental workload assessment: Class 1 - low workload perception & high self-evaluation group (n = 41, 3.1%); Class 2 - medium workload perception & medium self-evaluation group (n = 455, 34.0%); and Class 3 - high workload perception & low self-evaluation group (n = 841, `62.9%). Nurses belonging into class 3 were most likely to be older and have longer professional years, and displayed higher scores of fatigue and work engagement compared with the other latent classes (p < 0.05). Multivariate analysis showed that high cognitive workload increased subjective fatigue, and mental workload may be positively associated with work engagement. Group comparison results indicated that COVID-19 exposure contributed to significantly higher mental workload levels.

Conclusions: The complex scenario for the care of patients with infectious diseases, especially during an epidemic, raises the need for improved consideration of nurses' perceived workload, as well as their physical fatigue, work engagement and personal safety when working in public health emergencies.
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http://dx.doi.org/10.1186/s12912-021-00726-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532096PMC
October 2021

Stroke risk and its association with quality of life: a cross-sectional study among Chinese urban adults.

Health Qual Life Outcomes 2021 Oct 9;19(1):236. Epub 2021 Oct 9.

Department of Neurology, Jiading District Central Hospital Affiliated Shanghai University of Medicine and Health Sciences, Shanghai, 201800, People's Republic of China.

Background: Stroke is a leading cause of mortality and disability worldwide. Most stroke risk studies focused on more established biological and pathophysiological risk factors such as hypertension and smoking, psychosocial factors such as quality of life are often under-investigated and thus less reported. The current study aims to estimate stroke risk and explore the impact of quality of life on stroke risk among a community sample of urban residents in Shanghai.

Methods: This cross-sectional study was conducted in Fengxian District of Shanghai City from December 2018 to April 2019. 4030 representative participants were recruited through a multistage, stratified, probability proportional to size sampling method and completed the study. Stroke risk was assessed using the Rapid Stroke Risk Screening Chart that included 8 risk factors for stroke. Quality of life was measured using the World Health Organization Quality of Life-brief version (WHOQOL-BREF).

Results: One-third of residents were at risk for stroke, including 14.39% at high risk, and 18.68% at middle risk. The top three most commonly reported risk factors were physical inactivity (37.30%), hypertension (25.38%), and smoking (17.32%). Quality of life and its four domains were all independently and significantly associated with stroke risk. Multinominal logistic regressions showed that a one-unit increase in the quality of life was associated with a decreased relative risk for middle-risk relative to low-risk of stroke by a factor of 0.988 (95% CI:0.979, 0.997, P = 0.007), and a decreased relative risk for high-risk relative to low-risk of stroke by a factor of 0.975 (95% CI:0.966, 0.984, P < 0.001).

Conclusions: Our findings showed an alarmingly high prevalence of stroke risk among the sample, which may require future intervention programs to focus on improving both biological and behavioral risk factors such as increasing physical activity, early diagnosis and treatment of hypertension, and smoking cessation, as well as improving psychosocial factors such as quality of life.
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http://dx.doi.org/10.1186/s12955-021-01868-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501711PMC
October 2021

MicroRNAs in Woody Plants.

Front Plant Sci 2021 31;12:686831. Epub 2021 Aug 31.

College of Forestry, Henan Agricultural University, Zhengzhou, China.

MicroRNAs (miRNAs) are small (∼21-nucleotides) non-coding RNAs found in plant and animals. MiRNAs function as critical post-transcriptional regulators of gene expression by binding to complementary sequences in their target mRNAs, leading to mRNA destabilization and translational inhibition. Plant miRNAs have some distinct characteristics compared to their animal counterparts, including greater evolutionary conservation and unique miRNA processing methods. The lifecycle of a plant begins with embryogenesis and progresses through seed germination, vegetative growth, reproductive growth, flowering and fruiting, and finally senescence and death. MiRNAs participate in the transformation of plant growth and development and directly monitor progression of these processes and the expression of certain morphological characteristics by regulating transcription factor genes involved in cell growth and differentiation. In woody plants, a large and rapidly increasing number of miRNAs have been identified, but their biological functions are largely unknown. In this review, we summarize the progress of miRNA research in woody plants to date. In particular, we discuss the potential roles of these miRNAs in growth, development, and biotic and abiotic stresses responses in woody plants.
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http://dx.doi.org/10.3389/fpls.2021.686831DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438446PMC
August 2021

Quercetin reverses chronic unpredictable mild stress-induced depression-like behavior in vivo by involving nuclear factor-E2-related factor 2.

Brain Res 2021 12 13;1772:147661. Epub 2021 Sep 13.

College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China.

Quercetin is a flavonoid compound rich in many natural plants with a wide range of pharmacological effects and nutritional value. Although previous studies have initially shown the antidepressant effect of quercetin in some models. However, the exact mechanism of the antidepressant effect of quercetin on the depression model induced by chronic unpredictable mild stress (CUMS) is still unclear or has not been clearly elucidated. The present study aimed to investigate the antidepressant effect of quercetin in vivo on a CUMS-induced depression model that is closest to human depression, and to explore its mechanism of action around nuclear factor-E2-related factor 2 (Nrf2) related signaling pathways, for the first time. Our results demonstrated that CUMS for 21 consecutive days caused significant decreases in the sucrose preference, and the horizontal score and vertical score in the open field test of mice respectively by 22.6%, 34.4%, and 66.6% (all P < 0.01), and a significant increase in the immobility time during the forced swimming test by 110.5% (P < 0.01), but fortunately, after chronic oral administration of high dose quercetin at 40 mg/kg, the abnormalities of the above indicators were significantly reversed by 26.2%, 40.1%, 152.7%, 43.5% (all P < 0.01). Further western blot analysis showed that CUMS caused the phosphorylation or expression levels of phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), Nrf2 and heme oxygenase-1 (HO-1) proteins in the hippocampus of mice to significantly down-regulate by 60.0%, 72.1%, 90.0% and 50.1% (all P < 0.01), while after chronic oral administration of high dose quercetin at 40 mg/kg, the abnormalities of these proteins were significantly up-regulated by 85.8%, 182.0%, 325.1% and 60.3% (all P < 0.01). In addition, CUMS also caused significant reduction in the levels of antioxidants including superoxide dismutase (SOD) and glutathione-s transferase (GST) in the mice hippocampus by 51.3%, 40.3% (both P < 0.01), while after chronic oral administration of high dose quercetin at 40 mg/kg, the abnormalities of the above indicators were significantly reversed by 69.2% and 49.5% (both P < 0.01), as well as significant elevation in the levels of lipid peroxide malondialdehyde (MDA), inflammation medium nitric oxide (NO) and inducible nitric oxide synthase (iNOS) by 156.4%, 255.4% and 72.7% (all P < 0.01), while after chronic oral administration of high dose quercetin at 40 mg/kg, the abnormalities of the above indicators were significantly reversed by 45.9%, 26.8% and 55.2% (all P < 0.01). The medium dose of quercetin (20 mg/kg) only reversed some of the above indicators, while the low dose of quercetin (10 mg/kg) had no reversal effect on the above indicators. Collectively, the present study confirmed for the first time that quercetin weakened CUMS-induced depression in vivo, and its mechanism was at least partially attributable to the upregulation of hippocampal Nrf2 and the inhibition of iNOS, thereby correcting the central inflammatory response, and the imbalance between oxidation and antioxidant.
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http://dx.doi.org/10.1016/j.brainres.2021.147661DOI Listing
December 2021

Antidepressant effect of catalpol on corticosterone-induced depressive-like behavior involves the inhibition of HPA axis hyperactivity, central inflammation and oxidative damage probably via dual regulation of NF-κB and Nrf2.

Brain Res Bull 2021 Dec 6;177:81-91. Epub 2021 Sep 6.

College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China.

This study aimed to investigate the antidepressant effect and mechanism of catalpol on corticosterone (CORT)-induced depressive-like behavior in mice for the first time. As a result, CORT injection induced depressive-like behaviors of mice in behavioral tests, aggravated the serum CORT, adrenocorticotropic hormone, and corticotropin-releasing hormone levels, and conspicuously elevated the phosphorylations of nuclear factor kappa-B (NF-κB) in the hippocampus and frontal cortex, and down-regulated the expression levels of nuclear factor erythroid-2-related factor 2 (Nrf2). Furthermore, CORT exposure dramatically augmented the levels of inflammatory factors (interleukin-1β, tumor necrosis factor-α, nitric oxide synthase, and nitric oxide) and lipid peroxidation product malondialdehyde, and attenuated the levels of antioxidants including reduced glutathione, glutathione S-transferase, total superoxide dismutase, and heme oxygenase-1 in the mouse hippocampus and frontal cortex. On the contrary, catalpol administration markedly suppressed the abnormalities of the above indicators. From the overall results, this study displayed that catalpol exerted a beneficial effect on CORT-induced depressive-like behavior in mice possibly via the inhibition of hypothalamus-pituitary-adrenal (HPA) axis hyperactivity, central inflammation and oxidative damage at least partially through dual regulation of NF-κB and Nrf2.
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http://dx.doi.org/10.1016/j.brainresbull.2021.09.002DOI Listing
December 2021

The effect of evidence-based cluster nursing intervention on the incidences of delirium and the prognoses of ICU patients.

Am J Transl Res 2021 15;13(7):8309-8315. Epub 2021 Jul 15.

Department of Nursing, Shenzhen Longhua District Central Hospital Shenzhen 518110, China.

Objective: This study explored the effect of evidence-based cluster nursing intervention on the incidences of delirium and the prognoses of ICU patients.

Methods: 108 ICU patients admitted to our hospital from January 2020 to June 2020 were recruited as the study cohort and randomly divided into a control group and an observation group, with 54 cases in each group. The patients in the control group underwent routine nursing intervention, and the patients in the observation group underwent evidence-based cluster nursing intervention. The incidences of delirium, the durations of the mechanical ventilation, the ICU hospitalization durations, as well as the satisfaction levels in the two groups of patients were compared.

Result: The incidences of delirium in the observation group were conspicuously lower than they were in the control group (14.81%, 40.74%, = 9.0462, P = 0.0026). The duration of the delirium in the observation group was conspicuously lower than it was in the control group (2.87 ± 1.28), (5.21 ± 1.33), t = 9.3155, P = 0.0000. The durations of the mechanical ventilation and the ICU hospitalizations in the observation group were conspicuously shorter than they were in the control group. The differences were statistically significant (P < 0.05). The nursing satisfaction levels in the observation group were conspicuously higher than they were in the control group (94.44%, 72.22%, = 9.6000, P = 0.0019). There was no significant difference in the in-hospital mortality between the two groups (X = 2.1862, 0.1393).

Conclusion: Evidence-based cluster nursing intervention can conspicuously reduce the incidences of delirium, shorten the durations of the mechanical ventilation and the ICU stays, and improve patient prognosis, so it is worthy of clinical application.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8340266PMC
July 2021

Targeting ATF4-dependent pro-survival autophagy to synergize glutaminolysis inhibition.

Theranostics 2021 25;11(17):8464-8479. Epub 2021 Jul 25.

Department of Medical Oncology, Sir Run Run Shaw Hospital, Medical School of Zhejiang University, Hangzhou, China.

As glutamine plays a central role in cancer metabolism, inhibition of glutaminolysis has become an ideal anticancer therapeutic target. However, glutaminolysis inhibition leads to activation of autophagy, which compromises its antitumor effect. Hence, we investigated the mechanism underlying glutaminolysis inhibition-induced pro-survival autophagy. High-throughput sequencing was performed on colorectal cancer (CRC) cells before and after glutaminolysis inhibition to identify differentially expressed genes. Activating transcription factor 4 (ATF4) pathway enrichment in glutaminolysis inhibited cells was identified through gene set enrichment analysis. ATF4 expression was assessed by quantitative real-time PCR (qRT-PCR) and western blotting. The function of ATF4 on mechanistic target of rapamycin (mTOR) regulation was assessed by western blotting. Luciferase reporter assays and chromatin immunoprecipitation were used to confirm the regulation of DNA damage inducible transcript 4 (DDIT4) by ATF4. mRNA half-life assays, RNA immunoprecipitation, qRT-PCR and western blotting were performed to determine the relationship between FTO alpha-ketoglutarate dependent dioxygenase (FTO), YTH N-methyladenosine RNA binding protein 2 (YTHDF2), and ATF4. ATF4 regulation of pro-survival autophagy was measured by tandem monomeric red fluorescent protein-green fluorescent protein fluorescence microscopy. Finally, the synergistic effect of autophagy and glutaminolysis inhibition was analyzed in an azoxymethane/dextran sodium sulfate mouse model. The ATF4 pathway was activated in CRC cells upon glutaminolysis inhibition. Functionally, ATF4 transcriptionally upregulated DDIT4 to suppress mTOR, which induced pro-survival autophagy during glutaminolysis inhibition. Interestingly, glutaminolysis inhibition promoted mRNA expression by abrogating N-methyladenosine (mA) modification and YTHDF2-mediated RNA decay. Finally, inhibition of ATF4-induced autophagy enhanced the antitumor efficacy of glutaminolysis inhibition. Glutaminolysis inhibition upregulated expression in an mA-dependent manner to activate pro-survival autophagy through transcriptional activation of the mTOR inhibitor DDIT4. Targeting ATF4-induced autophagy is a new strategy to synergize glutaminolysis-targeting therapies for cancer treatment.
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http://dx.doi.org/10.7150/thno.60028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343999PMC
July 2021

Characterization of Human Origin Serovar 1,4,[5],12:i:- in Eastern China, 2014 to 2018.

Foodborne Pathog Dis 2021 11 20;18(11):790-797. Epub 2021 Jul 20.

Department of Food Safety and Assessment, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China.

The prevalence of serovar 1,4,[5],12:i:- among diarrhea patients has increased considerably in many countries around the world, including China. However, the characterization of this serovar of human origin has been less reported from China. We characterized 76 isolates of 1,4,[5],12:i:- gained from diarrhea patients from 2014 to 2018 in the Jiangsu Province of eastern China. These isolates fell into a single-sequence type (ST34) determined by multilocus sequence typing (MLST), and into 44 pulsed-field gel electrophoresis band patterns, with 1 pattern (JSSMM015) comprising 12 isolates (15.9%). By means of PCR-based assays, the seven prophage located virulence genes were detected in our 1,4,[5],12:i:- isolates with a high rate of , , , , , and (93.4-97.4%), and with a moderate rate of (42.1%). In contrast, none of the five plasmid-borne virulence genes (, , , , and ) was identified. We tested the isolates' susceptibility to 18 antibiotics of 9 categories using the VITEK 2 system. A high proportion (89.5%) of the isolates were multidrug resistant (MDR) strains with full resistance to cefazolin, cefotetan, amikacin, gentamycin, and tobramycin, followed by resistance to ampicillin (88.2%) and ampicillin/sulbactam (80.3%). The resistance to piperacillin/tazobactam, ceftazidime, cefepime, and levofloxacin was scarce (2.6-9.2%). Notably, an isolate from 2018 was resistant to carbapenems. and were the most common drug resistance genes presented in cephalosporin- and fluoroquinolone-resistant strains. All 1,4,[5],12:i:- isolates were capable of forming biofilm, with 13.2% of them having strong ability. However, no association was indicated between the scale of biofilm formation ability and MDR. Our results indicate that the combination of these characteristics may together provide a selective and competitive advantage to those 1,4,[5],12:i:- isolates, contributing to their increasing prevalence observed worldwide.
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http://dx.doi.org/10.1089/fpd.2021.0008DOI Listing
November 2021

Online preconcentration of lysozyme in hen egg white using responsive polymer coating in CE.

J Sep Sci 2021 Sep 26;44(18):3477-3488. Epub 2021 Jul 26.

CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, P. R. China.

A mixed polymer brushes material based on poly (2-methyl-2-oxazoline)- and poly (acrylic acid)-coated capillary with switchable protein adsorption/desorption properties was applied for online preconcentration of lysozyme in hen egg white during capillary electrophoresis performance. First, lysozyme in simulated egg white was successfully online preconcentrated and the detection signal of lysozyme was amplified. Ovalbumin, ovomucoid, and conalbumin in egg white were verified show negligible interference on the online preconcentration of lysozyme according to the study on electroosmotic flow mobility. Second, a series validation procedure was carried out to evaluate the proposed method performance. There was a good linearity behavior range from 0.1 to 5.0 ng/mL, limit of detection was 20 pg/mL, and limit of quantity was 50 pg/mL, the accuracy and robustness of this method were also excellent. Last, the proposed method has been successfully used to detect and analyze lysozyme in hen egg white, the determined amounts of lysozyme in hen egg white were consistent with reported normal levels and recoveries were in the range of 96.0-99.2%. After 75 consecutive runs, this prepared capillary was still stable for online preconcentration and determination of lysozyme in hen egg white without being affected by complex matrix.
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http://dx.doi.org/10.1002/jssc.202100246DOI Listing
September 2021

[Application of mixed polymer brushes based on poly(2-methyl-2-oxazoline) and poly(acrylic acid) to on-line preconcentration of lysozyme by capillary electrophoresis].

Se Pu 2020 Sep;38(9):1085-1094

Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, China.

A capillary coated with mixed polymer brushes that shows switchability toward lysozyme adsorption was developed. This capillary was applied for the on-line preconcentration of lysozyme by capillary electrophoresis (CE) in order to enhance the detection sensitivity. First, poly(2-methyl-2-oxazoline) (PMOXA) and poly(acrylic acid) (PAA) were synthesized by cationic ring-opening polymerization and reversible addition-fragmentation chain transfer (RAFT) polymerization, respectively. Then, glycidyl methacrylate (GMA) and PMOXA were used to prepare poly(2-methyl-2-oxazoline)-random-glycidyl methacrylate (PMOXA-r-GMA) via radical copolymerization, and poly(acrylic acid)-block-poly(glycidyl methacrylate) (PAA-b-PGMA) was obtained by the RAFT polymerization of GMA and PAA. A mixed solution of PMOXA-r-GMA and PAA-b-PGMA at a certain mass ratio was then injected into the capillary. Subsequent annealing provided capillary materials coated with mixed polymer brushes based on PMOXA and PAA. X-ray photoelectron spectroscopy (XPS) analysis was performed to determine the surface composition of the capillary raw materials. When the mass concentration of the mixed solution was 20 g/L and the mass ratio of PMOXA-r-GMA and PAA-b-PGMA was 1:1, the carboxyl content in the coating increased with increasing chain length of PAA. Fluorescein isothiocyanate-labeled lysozyme (FITC-lysozyme) adsorption assay demonstrated that the coated capillary had switchable properties for lysozyme adsorption upon pH and ionic strength () trigger. At pH 7 (=10mol/L), the capillary could adsorb a large amount of lysozyme, which could be released at pH 3 (=10mol/L). Subsequently, this coated capillary was applied to the on-line preconcentration of lysozyme by CE. The sensitivity enhancement factor was 17.69 when the chain length of PAA was 2.2 times that of PMOXA. The limit of detection could reach 8.7×10g/L. On-line preconcentration of lysozyme was performed for five successive times on the same day and on five consecutive days. The intraday and interday relative standard deviations (RSDs) for the peak areas were 2.9% and 4.1%, respectively. The intraday and interday RSDs for the migration times were 0.9% and 2.1%, respectively. The developed method for the preparation of the coated capillary with good stability only needs one step in this work, and this research will supply a simple and effective way to analyze trace protein by CE.
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http://dx.doi.org/10.3724/SP.J.1123.2020.02027DOI Listing
September 2020

[Application of poly(2-methyl-2-oxazoline) in protein separation by capillary electrophoresis].

Se Pu 2020 Sep;38(9):1022-1027

Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, China.

Capillary electrophoresis (CE), a commonly used liquid-phase separation technology, has many advantages such as high analysis speed, high separation efficiency, and low sample consumption. Hence, CE has gained popularity in food analysis, medical clinical diagnosis, environmental monitoring, and biological sample separation, especially in the field of protein separation and analysis. However, the fused silica capillaries that are commonly used in CE easily adsorb proteins, resulting in unstable electroosmotic flow and poor reproducibility of the separation results. In addition, due to the short optical path of the typical ultraviolet detectors employed in commercial CE, the detection sensitivity often does not meet the requirements for the direct analysis of low-abundance proteins. Therefore, developing a coating that can prevent protein adsorption and improve detection sensitivity is one of the important challenges in CE separation and analysis of proteins. Poly(2-methyl-2-oxazoline), a peptide-like hydrophilic polymer, not only has hydrophilicity, protein-repellent ability, and biocompatibility similar to the gold standard of the anti-protein adsorption polymer (polyethylene glycol), but also shows better stability than polyethylene glycol due to its peptide-like structure. Therefore, it has been increasingly used in biomass transfer, drug carrier, and impedance protein adsorption in recent years. This article aims to review the recent applications of poly(2-methyl-2-oxazoline) in CE from two standpoints. First, poly(2-methyl-2-oxazoline) was grafted onto the capillary inner wall using polydopamine as an anchor. The resulting coated capillary successfully separated a mixture of proteins (such as lysozyme, cytochrome C, ribonuclease A, and α -pancreas chymosinogen A), in addition to preventing the non-specific adsorption of other proteins during the quantitative analysis of melamine and lactoferrin in milk powder. Thus, the detection efficiency of melamine and lactoferrin in milk powder was improved. Second, poly(2-methyl-2-oxazoline) was used to produce a binary mixed brush coating with a stimulus-responsive polymer (such as polyacrylic acid). The capillary coated with the mixed brushes could adsorb high amounts of the target protein (such as bovine serum albumin and lysozyme) under certain pH and ionic strength conditions, and most of the adsorbed proteins could be desorbed by changing the pH and ionic strength. During the release, poly(2-methyl-2-oxazoline) present on the coating would prevent the adsorption of proteins. Under the dual effects of electroosmotic flow and electrophoresis, the released protein could migrate rapidly, and the instantaneous concentration of the protein reaching the detector could be greatly increased. Therefore, the target proteins could be on-line concentrated and the detection signals could be amplified, resulting in improved detection sensitivity for the protein. Future development trends in the function of poly(2-methyl-2-oxazoline) for the separation of proteins by CE are also discussed.
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http://dx.doi.org/10.3724/SP.J.1123.2020.02028DOI Listing
September 2020

Epstein-Barr virus (EBV) hyperimmune globulin isolated from donors with high gp350 antibody titers protect humanized mice from challenge with EBV.

Virology 2021 09 20;561:80-86. Epub 2021 Jun 20.

Medical Virology Section, Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, MD, 20892, USA. Electronic address:

Primary infection with Epstein-Barr virus (EBV) is associated with post-transplant lymphoproliferative disease and severe disease in patients with X-linked lymphoproliferative disease; no therapies are approved to prevent EBV infection in these patients. Hyperimmune globulin has been used to prevent some virus infections in immunocompromised persons. Here, we identified plasma donors with high titers of EBV gp350 and EBV B cell neutralizing antibodies. Pooled IgG isolated from these donors was compared to intravenous immunoglobulin (IVIG) for its ability to reduce viral load in the blood in humanized mice challenged with EBV. Mice that received EBV hyperimmune globulin had significantly reduced EBV DNA copy numbers compared to animals that received saline control; however, while animals that received EBV hyperimmune globulin had lower EBV DNA copies than those that received IVIG, the difference was not significant. Thus, while EBV hyperimmune globulin reduced viral load compared to IVIG, the effect was modest.
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http://dx.doi.org/10.1016/j.virol.2021.06.006DOI Listing
September 2021

Tissue Implantation Model of Tissue Implantation Model of Lidocaine (LCH)-Encapsulated Dextran (DEX)/Glycol (GLY) Nanoparticles Delivery for Pain Management.

J Biomed Nanotechnol 2021 Jun;17(6):1208-1216

Pain Department, Weifang People's Hospital, Weifang-261041, Shandong, P. R. China.

Lidocaine-loaded nanoparticles are versatile nanomaterials that may be used in pain treatment due to their wound healing properties. The current study describes a wound dressing formulation focused on lidocaine-loaded dextran/ethylene glycol nanoparticles (an anesthetic drug). The lidocaine-loaded dextran/ethylene glycol membranes were fabricated using lidocaine solutions inside the dextran/ethylene glycol medium. The influence of various experimental conditions on dextran/ethylene glycol nanoparticle formations were examined. The sizes of dextran/ethylene glycol and lidocaine-loaded dextran/glycol nanoparticles were examined through the HR-SEM. Moreover, the efficacy antibacterial activity of dextran/glycol and lidocaine-loaded dextran/ethylene glycol nanoparticles was evaluated against the microorganisms grampositive and negative. Furthermore, we observed the wound healing of wounds in skin using a mice model over a 16 days period. In this difference to the wounds of untreated mouse, quick healing was observed in the lidocaine-loaded dextran/glycol nanoparticles-treated wounds with fewer injury. These results specify that lidocaine-loaded dextran/ethylene glycol nanoparticles-based dressing material could be a ground-breaking nanomaterial having wound repair and implantations potential required for wound injury in pain management, which was proven using an animal model.
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http://dx.doi.org/10.1166/jbn.2021.3040DOI Listing
June 2021

Canonical Wnt Signaling Pathway on Polarity Formation of Utricle Hair Cells.

Neural Plast 2021 22;2021:9950533. Epub 2021 May 22.

NHC Key Laboratory of Hearing Medicine (Fudan University), Shanghai 200031, China.

As part of the inner ear, the vestibular system is responsible for sense of balance, which consists of three semicircular canals, the utricle, and the saccule. Increasing evidence has indicated that the noncanonical Wnt/PCP signaling pathway plays a significant role in the development of the polarity of the inner ear. However, the role of canonical Wnt signaling in the polarity of the vestibule is still not completely clear. In this study, we found that canonical Wnt pathway-related genes are expressed in the early stage of development of the utricle and change dynamically. We conditionally knocked out -catenin, a canonical Wnt signaling core protein, and found that the cilia orientation of hair cells was disordered with reduced number of hair cells in the utricle. Moreover, regulating the canonical Wnt pathway (Licl and IWP2) in vitro also affected hair cell polarity and indicated that Axin2 may be important in this process. In conclusion, our results not only confirm that the regulation of canonical Wnt signaling affects the number of hair cells in the utricle but also provide evidence for its role in polarity development.
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http://dx.doi.org/10.1155/2021/9950533DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166501PMC
January 2022

Environment-Driven Adaptations of Leaf Cuticular Waxes Are Inheritable for .

Front Plant Sci 2021 17;12:620245. Epub 2021 May 17.

College of Agronomy and Biotechnology, Southwest University, Chongqing, China.

Cuticular waxes covering the plant surface play pivotal roles in helping plants adapt to changing environments. However, it is still not clear whether the responses of plant cuticular waxes to their growing environments are inheritable. We collected seeds of (a perennial legume) populations from 30 growing sites in northern China and examined the variations of leaf cuticular waxes in a common garden experiment. Four wax genes, , , and , involved in biosynthesis of predominant wax classes (primary alcohol and alkane) and wax precursors, were isolated to test the contributions of genetic variations of the coding sequences (CDS) and the promoter sequences and epigenetic modifications. The plasticity responses of the cuticular waxes were further validated by two stress-modeling experiments (drought and enhancing ultraviolet B). Great variations in total wax coverage and abundance of wax classes or wax compounds were observed among populations in a common garden experiment. Stress-modeling experiments further validated that would alter leaf wax depositions under changed growing conditions. The transcriptional levels of the wax genes were positively or negatively correlated with amounts of cuticular waxes. However, the analysis of promoter methylation showed that the methylation level of the promoter region was not associated with their expressions. Although both promoter sequences and CDS showed a number of polymorphic sites, the promoters were not naturally selected and insignificant difference could be observed in the numbers and types of acting elements of the four wax genes among populations. In contrast, the CDS of the wax genes were naturally selected, with a number of missense mutations resulting in alterations of the amino acid as well as their isoelectric points and polarities, which could impact on enzyme function/activity. We conclude that long-term adaptation under certain environments would induce genetic mutation of wax biosynthesis genes, resulting in inheritable alterations of cuticular wax depositions.
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http://dx.doi.org/10.3389/fpls.2021.620245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165318PMC
May 2021

Risk Prediction Model of Gestational Diabetes Mellitus in a Chinese Population Based on a Risk Scoring System.

Diabetes Ther 2021 Jun 15;12(6):1721-1734. Epub 2021 May 15.

Department of Endocrinology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, No. 321, Zhongshan Road, Nanjing, 210008, China.

Introduction: Gestational diabetes mellitus (GDM) is associated with adverse perinatal outcomes. Accurate models for early prediction of GDM are lacking. This study aimed to explore an early risk prediction model to identify women at high risk of GDM through a risk scoring system.

Methods: This was a retrospective cohort study of 785 control pregnancies and 855 women with GDM. Maternal clinical characteristics and biochemical measures were extracted from the medical records. Logistic regression analysis was used to obtain coefficients of selected predictors for GDM in the training cohort. The discrimination and calibration of the risk scores were evaluated by the receiver-operating characteristic (ROC) curve and a Hosmer-Lemeshow test in the internal and external validation cohort, respectively.

Results: In the training cohort (total = 1640), two risk scores were developed, one including predictors collected at the first antenatal care visit for early prediction of GDM, such as age, height, pre-pregnancy body mass index, educational background, family history of diabetes, menstrual history, history of cesarean delivery, GDM, polycystic ovary syndrome, hypertension, and fasting blood glucose (FBG), and the total risk score also including FBG and triglyceride values during 14-20 gestational weeks. Our total risk score yielded an area under the curve (AUC) of 0.845 (95% CI = 0.805-0.884). This performed better in an external validation cohort, with an AUC of 0.886 (95% CI = 0.856-0.916).

Conclusion: The GDM risk score, which incorporates several potential clinical features with routine biochemical measures of GDM, appears to be a sensitive and reliable screening tool for earlier detection of GDM risk.
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http://dx.doi.org/10.1007/s13300-021-01066-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179863PMC
June 2021

Conductive hearing loss in large vestibular aqueduct syndrome -clinical observations and proof-of-concept predictive modeling by a biomechanical approach.

Int J Pediatr Otorhinolaryngol 2021 Jul 29;146:110752. Epub 2021 Apr 29.

ENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China; NHC Key Laboratory of Hearing Medicine, Fudan University, Shanghai, 200031, China. Electronic address:

Objective: The purpose of this study was to investigate the effect of a dilated vestibular aqueduct on conductive hearing loss (CHL). A biomechanical method was proposed for modeling the patterns of CHL in patients with large vestibular aqueduct syndrome (LVAS).

Study Design: High resolution computed tomography (CT) scans and pure tone audiometry (PTA) were retrospectively collected from 16 patients who were diagnosed with LVAS. Seventeen ears with measurable air-bone gaps (ABGs) on PTA were applied for model development. The sizes of midpoint, operculum and distal segment were measured from CT to calculate the biomechanical parameters of each vestibular aqueduct. The mechanical effect of the dilated vestibular aqueduct on sound conduction was simulated using a lumped-parameter model. The CHL levels predicted by the model were compared with the observed ABGs at 250, 500 and 1000 Hz respectively.

Results: The model was able to predict the trend that greater ABGs occurred at lower frequencies, which were consistent with clinical observations. However, deviations between the predicted and the observed ABGs became larger as the frequency increased. None of the correlation coefficients between the radiologic measures and the observed ABG levels were significant.

Conclusion: These findings lend support to the feasibility of this approach in modeling CHL in LVAS. The presence of a dilated vestibular aqueduct leads to altered impedance and sound pressure, suggesting the impact of a pathological third window. High individual variability of the observed ABGs implies additional factors may also be involved, especially at 500 Hz and 1000 Hz.
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http://dx.doi.org/10.1016/j.ijporl.2021.110752DOI Listing
July 2021

Comparison of Endoscopic Cartilage Myringoplasty in Dry and Wet Ears With Chronic Suppurative Otitis Media.

Ear Nose Throat J 2021 Mar 15:145561321999263. Epub 2021 Mar 15.

ENT Institute and Otorhinolaryngology Department, Affiliated Eye and ENT Hospital, Fudan University, Shanghai, People's Republic of China.

Objectives: This study compared the rate of graft success, as well as hearing improvement and dry ear time between dry ears and wet ears with otomycosis or without otomycosis in patients with chronic suppurative otitis media (CSOM) after endoscopic cartilage myringoplasty.

Methods: This retrospective study was conducted in a tertiary hospital in Shanghai. In total, 83 patients with CSOM (43 with dry ears and 40 with wet ears) were included. Among the 40 patients with CSOM and wet ears, 25 exhibited otomycosis. All patients underwent endoscopic myringoplasty, and perforations were repaired using tragal cartilage with a single-sided perichondrium. Patients were followed up for at least 6 months. Pure-tone hearing was examined preoperatively and at 3 months postoperatively. The graft uptake rate, hearing improvement, and dry ear time were compared between the groups.

Results: The graft success rate did not differ significantly between the dry-ear and wet-ear groups (95.35% and 90.00%, respectively). Furthermore, the graft success rate also did not differ significantly between patients with wet ears and otomycosis and those with wet ears without otomycosis (92.00% and 86.67%, respectively). Hearing gain did not differ significantly between the dry-ear and wet-ear groups. No significant difference in hearing gain was also found in patients with wet ears with or without otomycosis. However, the time to dry ear was significantly longer in the wet-ear group than in the dry-ear group.

Conclusion: Patients with CSOM and wet ears required more time to achieve a completely healthy status. However, the graft success rate and hearing improvement were not affected by a wet middle ear and otomycosis. Thus, endoscopic myringoplasty using tragus cartilage is an effective treatment for refractory CSOM in patients with wet ears and otomycosis.
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http://dx.doi.org/10.1177/0145561321999263DOI Listing
March 2021

Ultraviolet-light-triggered isomerization of Rydberg-excited propanal: Real-time capture of ultrafast structural evolution and dynamics investigation.

J Chem Phys 2021 Feb;154(5):054301

State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, People's Republic of China.

Structure rearrangement processes, such as isomerization, are attracting extensive interest as a potential carrier in molecular scale electronics design. UV-light-triggered isomerization of Rydberg-excited propanal with two UV photons has been investigated with time-resolved photoelectron spectroscopy. By following the photoionization from 3s Rydberg states in the time domain, the ultrafast structural evolution and the corresponding photoisomerization dynamics are observed and tracked in real-time. The conversion barrier for isomerization from cis-propanal to gauche isomer is estimated to be about 1500 ± 100 cm experimentally. Both the photoisomerization yield and the conversion rate have shown strong dependence on the excitation energy. It is observed that whether vibration modes are selectively excited or not, cis-to-gauche photoisomerization of propanal in 3s Rydberg state occurs once the excitation energy is higher than the conversion barrier without any vibrational excitation specificity. This yields a powerful approach to studying structural evolution dynamics in large molecules, which may have applications in molecular devices.
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http://dx.doi.org/10.1063/5.0039802DOI Listing
February 2021

The complete mitochondrial genome of (Jordan & Snyder, 1901).

Mitochondrial DNA B Resour 2020 Aug 12;5(3):3154-3156. Epub 2020 Aug 12.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou, China.

is one of the species in the China-Vietnam Collective Fishery Zone, which has a few relevant studies. In this study, the mitochondrial genome of was determined for the first time using next-generation sequencing; the overall base components of mitogenome consisting of 17,784 bp was 32.45% for A, 25.76% for T, 15.72% for G, 26.08% for C, and its GC content was 41.8%. The mitochondrial circular genome was composed of 13 protein-coding genes, 22 transfer RNAs, 2D-loop, and 2 ribosomal RNAs. Polygenetic analysis showed that the was very close to . It can provide data reference for the analysis of genetic evolution of this species.
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http://dx.doi.org/10.1080/23802359.2020.1806130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782292PMC
August 2020

Subliminal affective priming effect: Dissociated processes for intense versus normal facial expressions.

Brain Cogn 2021 03 31;148:105674. Epub 2020 Dec 31.

Guangdong Provincial Key Laboratory of Social Cognitive Neuroscience and Mental Health, Department of Psychology, Sun Yat-Sen University, Guangzhou 510006, China; Peng Cheng Laboratory, Shenzhen 518055, China. Electronic address:

Positive vs. negative intense-facial expressions are difficult to explicitly distinguish; yet, whether they dissociate when subliminally presented remains unclear. Through three experiments using affective priming paradigms, we assessed how intense facial expressions, when presented briefly (17 ms) and masked, influenced following neutral ambiguous words (Experiment 1) or visible facial expressions (Experiments 2&3). We also compared these results with those of using normal facial expressions as primes in each experiment. All experiments indicated masked affective priming effects (biasing valence judgement of neutral words or facilitating reaction time to faces with the same valence as the prime) in normal facial expression, but not those intense ones. Experiment 3 using event related potentials (ERPs) further revealed that two ERP components N250 and LPP were consistent with behavioral changes in the normal condition (larger when valences of primes and targets were different), but inconsistent in the intense condition. Taken together, our results provided behavioral and neural evidence for distinctive processing between normal and intense facial expressions under masked condition.
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http://dx.doi.org/10.1016/j.bandc.2020.105674DOI Listing
March 2021

Hair cell uptake of gentamicin in the developing mouse utricle.

J Cell Physiol 2021 07 23;236(7):5235-5252. Epub 2020 Dec 23.

ENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China.

Intratympanic injection of gentamicin has proven to be an effective therapy for intractable vestibular dysfunction. However, most studies to date have focused on the cochlea, so little is known about the distribution and uptake of gentamicin by the counterpart of the auditory system, specifically vestibular hair cells (HCs). Here, with a combination of in vivo and in vitro approaches, we used a gentamicin-Texas Red (GTTR) conjugate to investigate the mechanisms of gentamicin vestibulotoxicity in the developing mammalian utricular HCs. In vivo, GTTR fluorescence was concentrated in the apical cytoplasm and the cellular membrane of neonatal utricular HCs, but scarce in the nucleus of HCs and supporting cells. Quantitative analysis showed the GTTR uptake by striolar HCs was significantly higher than that in the extrastriola. In addition, the GTTR fluorescence intensity in the striola was increased gradually from 1 to 8 days, peaking at 8-9 days postnatally. In vitro, utricle explants were incubated with GTTR and candidate uptake conduits, including mechanotransduction (MET) channels and endocytosis in the HC, were inhibited separately. GTTR uptake by HCs could be inhibited by quinine, a blocker of MET channels, under both normal and stressed conditions. Meanwhile, endocytic inhibition only reduced GTTR uptake in the CoCl hypoxia model. In sum, the maturation of MET channels mediated uptake of GTTR into vestibular HCs. Under stressed conditions, MET channels play a pronounced role, manifested by channel-dependent stress enhanced GTTR permeation, while endocytosis participates in GTTR entry in a more selective manner.
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http://dx.doi.org/10.1002/jcp.30228DOI Listing
July 2021

Role of TGF-β1-mediated epithelial-mesenchymal transition in the pathogenesis of tympanosclerosis.

Exp Ther Med 2021 Jan 2;21(1). Epub 2020 Nov 2.

Department of Otorhinolaryngology Head and Neck Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China.

The present study aimed to explore the role of TGF-β1-mediated epithelial-mesenchymal transition (EMT) in the pathogenesis of tympanosclerosis. Sprague Dawley rats were injected with inactivated suspension to establish a rat model of tympanosclerosis. The rats were sacrificed 8 weeks after the model was established. H&E and von Kossa staining was used to observe the morphological changes of middle ear mucosa. Western blotting was used to detect the expression of TGF-β1 and EMT-associated proteins in the mucosa samples. Middle ear mucosal epithelial cells of rats were collected to establish a primary culture. The cultured cells were stimulated with TGF-β1 and the expression of EMT-associated proteins was detected by western blotting and immunofluorescence. In addition, the cells were treated with TGF-β receptor type I/II inhibitor and the expression level of EMT-associated proteins was detected by western blotting. Sclerotic lesions appeared on 72.4% of tympanic membranes, and marked inflammation, inflammatory cell infiltration and fibrosis were found in the middle ear mucosa of rat models of tympanosclerosis. In middle ear mucosa of rats with tympanosclerosis, the expression of mesenchymal cell markers increased and that of epithelial cell markers decreased compared with the control group. TGF-β1 stimulated the activation of the EMT pathway in middle ear mucosal epithelial cells, resulting in an increased expression of fibronectin and N-cadherin. In addition, a decreased expression level of EMT-associated proteins was observed when TGF-β1 was inhibited. In conclusion, the present study indicated that TGF-β1-mediated EMT may play an important role in the pathogenesis of tympanosclerosis.
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http://dx.doi.org/10.3892/etm.2020.9438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7678609PMC
January 2021

Rho guanine nucleotide exchange factor 39 increases the viability, migration and invasion of clear cell renal cell carcinoma cells via the activation of the AKT/ERK signaling pathway.

Genet Mol Biol 2020 18;43(4):e20190383. Epub 2020 Nov 18.

Jining No.1 People's Hospital, Department of Urinary Surgery, Shandong, China.

We attempted to explore the effect of Rho guanine nucleotide exchange factor 39 (ARHGEF39) on the phenotypes of clear cell renal cell carcinoma (ccRCC) cells and the underlying mechanism. Analyses of the data from The Cancer Genome Atlas (TCGA) illustrated that ARHGEF39 expression was upregulated in ccRCC and high ARHGEF39 expression was correlated with a worse prognosis. The mRNA and protein expression of ARHGEF39 in ccRCC and nontumorigenic cells was measured by qRT-PCR and western blotting, respectively. The results showed that ARHGEF39 expression was upregulated in ccRCC cells compared with nontumorigenic cells. CCK8 and clonogenic assays were used to measure the viability of ccRCC cells after knockdown or overexpression of ARHGEF39. Transwell assays were used to examine the changes in cell motility after alterations in ARHGEF39 expression and treatment with LY294002 (an AKT inhibitor) or PD98059 (an ERK inhibitor). ARHGEF39-mediated changes in the phosphorylation of AKT and ERK were measured by western blotting. The results indicated that ARHGEF39 promoted the viability, migration and invasion of ccRCC cells by regulating the activation of the AKT/ERK signaling pathway. Overall, our research suggested that ARHGEF39 was upregulated in ccRCC and possibly facilitated the malignant development of ccRCC by modulating the AKT/ERK signaling pathway.
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http://dx.doi.org/10.1590/1678-4685-GMB-2019-0383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7684694PMC
November 2020
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