Publications by authors named "Yanli Wang"

652 Publications

Genetic variations in ZmSAUR15 contribute to the formation of immature embryo-derived embryonic calluses in maize.

Plant J 2021 Nov 25. Epub 2021 Nov 25.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.

The ability of immature maize embryos to form embryonic calluses (ECs) is highly genotype-dependent, which limits transgenic breeding development in maize. Here, we report the association mapping-based cloning of ZmSAUR15 using an association panel (AP) consisting of 309 inbred lines with diverse formation abilities of EC. We demonstrated that ZmSAUR15, which encodes a small auxin-upregulated RNA, acts as a negative effector in maize EC induction. Polymorphisms in the ZmSAUR15 promoter that influence the expression of ZmSAUR15 transcripts modulate the EC induction capacity in maize. ZmSAUR15 is involved in indole-3-acetic acid biosynthesis and cell division in immature embryo-derived callus. The ability of immature embryos to induce EC formation can be improved by the knockout of ZmSAUR15, which consequently increases the callus regeneration efficiency. Our study provides new insights into overcoming the genotypic limitations associated with EC formation and improving genetic transformation in maize.
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http://dx.doi.org/10.1111/tpj.15609DOI Listing
November 2021

Integrative Analysis for Elucidating Transcriptomics Landscapes of Systemic Lupus Erythematosus.

Front Genet 2021 4;12:782005. Epub 2021 Nov 4.

Department of Rheumatology and Immunology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

Systemic lupus erythematosus (SLE) is a complex and heterogeneous autoimmune disease that the immune system attacks healthy cells and tissues. SLE is difficult to get a correct and timely diagnosis, which makes its morbidity and mortality rate very high. The pathogenesis of SLE remains to be elucidated. To clarify the potential pathogenic mechanism of SLE, we performed an integrated analysis of two RNA-seq datasets of SLE. Differential expression analysis revealed that there were 4,713 and 2,473 differentially expressed genes, respectively, most of which were up-regulated. After integrating differentially expressed genes, we identified 790 common differentially expressed genes (DEGs). Gene functional enrichment analysis was performed and found that common differentially expressed genes were significantly enriched in some important immune-related biological processes and pathways. Our analysis provides new insights into a better understanding of the pathogenic mechanisms and potential candidate markers for systemic lupus erythematosus.
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http://dx.doi.org/10.3389/fgene.2021.782005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599929PMC
November 2021

Lessons from Epstein-Barr virus DNA detection in cerebrospinal fluid as a diagnostic tool for EBV-induced central nervous system dysfunction among HIV-positive patients.

Biomed Pharmacother 2021 Nov 12;145:112392. Epub 2021 Nov 12.

Department of Infectious Diseases, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China. Electronic address:

Polymerase chain reaction (PCR) analysis of Epstein-Barr virus (EBV) DNA in cerebrospinal fluid (CSF) remains vital for evaluating active EBV infection involving the central nervous system (CNS). CSF EBV DNA was often found in conjunction with other microbial infection affecting the CNS among patients infected with human immunodeficiency virus (HIV). Sometimes CSF EBV DNA is detectable in patients without neurological symptoms. This review focused on the clinical and laboratory features of CNS EBV infection among patients with HIV, and discussed various types of EBV-associated CNS infections, and predominant neoplasms involving CNS such as primary central nervous system lymphoma (PCNSL), CNS-non-Hodgkin's lymphoma, smooth muscle tumors and leiomyosarcomas, EBV encephalitis or myelitis, EBV meningitis and EBV coinfection with other causative agents were also included. Furthermore, the metagenomic next-generation sequencing technique with high sensitivity for the detection of pathogenic coinfection in the CSF were also reviewed. We concluded that CSF EBV-DNA detection with high sensitivity and specificity could be a useful diagnostic tool for CNS lymphoma among HIV patients; however, it is still unknown for other CNS diseases. We further summarized and conclude that positive CSF EBV-DNA detection combined with specific brain focal lesions could be a minimally invasive method to diagnose PCNSL. The occurrence of positive CSF EBV-DNA was influenced by PCR detection limit, PCR methods, immunocompromised status, the possible influence of anti-herpetic therapy and anti-HIV therapy, and the size and location of a tumor mass. Uniform PCR methods as vital diagnostic tools and optimal EBV-DNA load threshold need to be established.
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http://dx.doi.org/10.1016/j.biopha.2021.112392DOI Listing
November 2021

Polymorphisms in lncRNA and susceptibility to breast cancer in Chinese population.

Aging (Albany NY) 2021 11 11;13(21):24360-24378. Epub 2021 Nov 11.

Department of Epidemiology and Statistics, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan Province, China.

Background: Published studies based on pharmacokinetics have explored the relationship between the lncRNA and the prognosis of breast cancer (BC) resistance and recurrence. However, the underlying association of SNPs with BC development remains unclear.

Methods: Combining bioinformatics and databases, SNPs (Single Nucleotide Polymorphisms) in the gene were screened, and SNPs in the lncRNA were selected for genotyping among 504 Chinese Han patients and 505 healthy controls, which were frequency-matched for age (±2 years). Logistic regression analysis was used to explore the association between SNPs and the BC risk. Interactions between the SNPs and reproductive factors were further evaluated using the multifactor dimensionality reduction (MDR) method. qRT-PCR was performed to detect expression in individuals with different genotypes of rs34841297. The target miRNA, miR-4456 of rs34841297 was predicted by websites and confirmed by performing dual luciferase gene reporter assays. CCK-8 and Transwell experiments were designed to explore the effects of miR-4456 on the proliferation, invasion and migration of BC cells.

Results: In this study, nine SNPs were screened. After adjusting for age, menarche age, menopausal status, number of pregnancies, history of abortions, breast feeding history and family history of BC, the results of the logistic regression analysis showed the rs34841297 A/- gene polymorphism was positively correlated with the incidence of BC. Compared with the AA genotype, patients with the A-+-- genotype of rs34841297 at age<50 years, and menarche age<14 years, Premenopausal status, history of abortion, no history of breastfeeding and no family history of tumors in first-degree relatives had an increased risk of BC. MDR results revealed that individuals with rs34841297 - (homozygous deletion) of the A allele who were not menopausal and had no history of breastfeeding had a higher risk of BC. qRT-PCR results revealed that homozygous deletion (1.68±1.37) of the rs34841297 A- genotype resulted in higher expression than the heterozygous deletion genotype (0.95±0.94) and wild AA genotype (0.26±0.12). Binding between and miR-4456 was occurred when rs34841297 carried the AA genotype. Moreover, preliminary functional studies indicated that the overexpression of miR-4456 increased the proliferation, invasion and migration of BC cells.

Conclusion: Our study showed that the gene polymorphism may be related to BC susceptibility, and the rs34841297 A/- genotype may probably affect the proliferation, invasion and migration of BC cells by modulating the interactions with of miR-4456.
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http://dx.doi.org/10.18632/aging.203686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8610136PMC
November 2021

Nitrous oxide-related neurological disorders: Clinical, laboratory, neuroimaging, and electrophysiological findings.

Brain Behav 2021 Nov 10:e2402. Epub 2021 Nov 10.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background: Recreational N O abuse is an important etiology of neurological impairment in young patients, which may easily be ignored clinically. Few current studies have investigated the characteristics or the effects experienced by its users. We aimed to explore any correlation between the clinical severity and biomarkers and spinal magnetic resonance imaging (MRI) abnormalities, identify independent factors associated with spinal MRI abnormalities, and ascertain factors affecting depression/anxiety in patients with N O-related neurological disorders.

Methods: Patients with N O-related neurological disorders were enrolled retrospectively between February 2017 and July 2020. Their demographic, clinical, laboratory, neuroimaging, electrophysiological, and neuropsychological findings were analyzed. Correlation analyses were conducted using Spearman's or Pearson's correlation and linear regression analysis. Independent factors associated with spinal MRI abnormalities were identified using univariate and multivariate analyses.

Results: The principal clinical manifestations of N O-related neurological disorders (n = 63; 38 men, 25 women; mean age ± SD: 22.60 ± 4.46 years) were sensory disturbance, followed by gait disturbance and pyramidal tract damage. A significant negative correlation existed between serum vitamin B levels and clinical severity (r = -0.309, p = .014), which disappeared after linear regression. An interval of less than 6 months between initial N O abuse and hospitalization was independently associated with spinal MRI abnormalities (39.47% vs. 72.00%, respectively; χ = 6.40, p = .01). Thirty-eight (60.32%) and 40 (63.49%) patients experienced anxiety and depression, respectively. Moreover, the higher the clinical scores/serum homocysteine levels, the greater the severity of anxiety/depression (r = 0.442, p < .01; r = 0.346, p < .01; r = 0.477, p < .01; r = 0.324, p < .01).

Conclusions: The significant inverse correlation between initial vitamin B levels and clinical severity could aid prognosis prediction in patients with N O-related neurological disorders. Spinal MRI abnormalities were not related to clinical severity but depended on the time interval between initial N O abuse and hospitalization. Anxiety and depression were common comorbidity in these patients, and their severity increased with the intensity of clinical impairment and/or serum homocysteine levels.
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http://dx.doi.org/10.1002/brb3.2402DOI Listing
November 2021

Burnout of Healthcare Workers Based on the Effort-Reward Imbalance Model: A Cross-Sectional Study in China.

Int J Public Health 2021 25;66:599831. Epub 2021 Feb 25.

Department of Epidemiology and Health Statistics, Xiangya School of Public Health, Central South University, Changsha, China.

The effort-reward imbalance (ERI) model is widely used in job stress research. However, few studies using this model have been conducted in developing countries. This study tested the extrinsic and intrinsic hypotheses regarding the burnout of healthcare workers in China with the ERI model. Job stress was assessed by Siegrist's ERI questionnaire, and burnout was evaluated by the Maslach Burnout Inventory-General Survey (MBI-GS). A total of 1,505 effective respondents were included in the final study. Multiple and hierarchical linear regression was used to analyze the association between components in the ERI model and burnout. Emotional exhaustion and cynicism were positively correlated with ERI and overcommitment. Professional efficacy was positively related to ERI but not to overcommitment. ERI was the determining factor of emotional exhaustion and cynicism. Overcommitment moderated the relationship between ERI and emotional exhaustion and between ERI and cynicism. Changing workplace conditions and increasing personal resilience might alleviate burnout among hospital workers in China. The links between professional efficacy and stressful work environment need further exploration.
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http://dx.doi.org/10.3389/ijph.2021.599831DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565289PMC
November 2021

[Retracted] Knockdown of AQP1 inhibits growth and invasion of human ovarian cancer cells.

Mol Med Rep 2021 Dec 22;24(6). Epub 2021 Oct 22.

Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that certain of the western blotting data shown in Fig. 2B and flow cytometric data shown in Fig. 3B bore unexpected similarities to data appearing in different form in other articles by different authors. Furthermore, there were additional concerns regarding the possibly anomalous appearance of cell migration data shown in Fig. 4B. Owing to the fact that some of the contentious data in the above article had already been published elsewhere, or were already under consideration for publication, prior to its submission to , the Editor has decided that this paper should be retracted from the Journal. After having been in contact with the authors, they agreed with the decision to retract the paper. The Editor apologizes to the readership for any inconvenience caused. [the original article was published in 16: 5499‑5504, 2017; DOI: 10.3892/mmr.2017.7282].
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http://dx.doi.org/10.3892/mmr.2021.12503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554380PMC
December 2021

Analysis of clinical characteristics and detection of pathogens in patients with pulmonary tuberculosis complicated with fungal infection.

Minerva Med 2021 Oct 21. Epub 2021 Oct 21.

Department of Respirology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China -

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http://dx.doi.org/10.23736/S0026-4806.21.07881-2DOI Listing
October 2021

Integrating Intrinsic Radiosensitivity and Immune Status for Predicting Benefits of Radiotherapy in Head and Neck Squamous Cell Carcinoma.

Med Sci Monit 2021 Oct 21;27:e932126. Epub 2021 Oct 21.

Department of Radiation Oncology, The First Hospital of China Medical University, Shenyang, Liaoning, China (mainland).

BACKGROUND HNSCC (head and neck squamous cell carcinoma) is a heterogeneous disease for which radiotherapy is a main treatment. As intrinsic radiosensitivity and immune status affect the initial and effective stage of the radiation-induced cancer immunity cycle, respectively, it is important to consider both of them when we select patients who can benefit from radiotherapy. MATERIAL AND METHODS Our study included all HNSCC patients with complete survival and radiotherapy information in TCGA database. Patients were divided into RS (radiosensitive), RR (radioresistant), immune, and non-immune groups according to their RSI (radiosensitivity index) and immune score calculated by the ESTIMATE algorithm. Survival analysis was performed to compare OS (overall survival) between patients receiving and not receiving radiotherapy. GO and KEGG pathway enrichment analysis was performed for functional analysis. Univariate Cox and ridge regression analysis were performed to construct a predictive gene signature based on the combined stratification. RESULTS Only patients in the RS-immune group could benefit from radiotherapy, and the survival analysis results remained consistent after we performed propensity score matching between patients receiving and not receiving radiotherapy. The differentially expressed genes between the RS-immune and non-RS-immune groups were mainly enriched in pathways related to immune process. The 3-gene signature we built exhibited predictive value in training and validation cohorts when treated as a binary or continuous variable. CONCLUSIONS The combined stratification of intrinsic radiosensitivity and immune status was superior to considering intrinsic radiosensitivity or immune status alone and could be used in preclinical evaluation to select patients or to decide whether radiotherapy sensitizers and immunotherapy should be used at the same time.
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http://dx.doi.org/10.12659/MSM.932126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8547194PMC
October 2021

Evaluation of aortic biomechanics in patients with aortic disease via imaging: A review.

Authors:
Yanli Wang Min Gong

J Clin Ultrasound 2021 Oct 20. Epub 2021 Oct 20.

Department of Cardiovascular Ultrasound, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.

As a bridge between the heart and the arteries, the aorta plays an important role in the cardiovascular system. The morbidity and mortality of aortic disease are extremely high, which is a serious threat to human life. The biomechanical abnormality of the aorta is an important factor of a series of pathological changes in the aortic wall. At present, there are many imaging methods to evaluate the biomechanics of the aorta, which will benefit to the early diagnosis and treatment of aortic disease. In this review, we describe the application of various imaging methods and parameters in aortic disease.
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http://dx.doi.org/10.1002/jcu.23087DOI Listing
October 2021

Current trends and future prospective in nanoremediation of heavy metals contaminated soils: A way forward towards sustainable agriculture.

Ecotoxicol Environ Saf 2021 Dec 11;227:112888. Epub 2021 Oct 11.

State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects and Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province. Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. Electronic address:

Heavy metals (HMs) contamination in agricultural soils is a major concern for global food safety and human health. Although, various in-situ and ex-situ remediation methods have been used for the treatment of HMs contaminated soils, however, they also have many drawbacks viz., capital investment, toxicity, and environmental health hazards. Consequently, there is an urgent need to develop a novel method to ameliorate the toxicity of HMs in agricultural soils. In recent years, nanoparticles (NPs) have gained significant attention due to their potential applications in the environment and agriculture fields. Nanoremediation employs NPs that effectively reduce the contents of toxic HMs in the soil-plant system. Several studies have reported that the application of NPs in HMs-polluted soils, which reduced plant-available HMs concentration soils. However, the long-term efficiency of NPs immobilization is still unclear. Here, we provide details about the toxicity of HMs to environmental systems and potential applications NPs to alleviate the accumulation of HMs in agricultural soils. Finally, we present the mechanistic route of HMs-toxicity alleviation in plants by NPs application as well as their long-term efficiency and future prospects.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112888DOI Listing
December 2021

Socioeconomic Status and Prosocial Behavior: The Mediating Roles of Community Identity and Perceived Control.

Int J Environ Res Public Health 2021 09 30;18(19). Epub 2021 Sep 30.

School of Psychology, Guizhou Normal University, Guiyang 550025, China.

Background: Previous studies have examined the association between socioeconomic status and prosocial behavior, but the underlying mechanism between them is unclear. The present study aimed to examine the serially mediating roles of community identity and perceived control in this relationship.

Methods: Using the convenient sampling technique, a total of 477 Chinese adults from Chinese communities, and ranging in age from 20 to 65 completed the questionnaires for objective socioeconomic status, the MacArthur scale of subjective socioeconomic status, an eight-item community identity scale, the perceived control scale, and a prosocial tendencies measure. Bivariate correlation analysis and regression analysis were used to examine the relationships among the major variables.

Results: Socioeconomic status was positively associated with prosocial behavior. It was also found that community identity and perceived control played mediating roles between socioeconomic status and prosocial behavior, respectively. In addition, community identity and perceived control also had a serially mediating role in the relationship.

Conclusions: Community identity and perceived control played a serially mediating role in the association between socioeconomic status and prosocial behavior. The findings in the present study contribute to understanding the underlying mechanism in the association between socioeconomic status and prosocial behavior among adults, and have important implications for interventions aimed at improving prosocial behavior in lower-status individuals.
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http://dx.doi.org/10.3390/ijerph181910308DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508049PMC
September 2021

The Optimal Time of Ovarian Reserve Recovery After Laparoscopic Unilateral Ovarian Non-Endometriotic Cystectomy.

Front Endocrinol (Lausanne) 2021 24;12:671225. Epub 2021 Sep 24.

Department of Obstetrics and Gynecology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai, China.

Background: Laparoscopic ovarian cystectomy is established as the standard surgical approach for the treatment of benign ovarian cysts. However, previous studies have shown that potential fertility can be directly impaired by laparoscopic ovarian cystectomy, diminished ovarian reserve (DOR), and even premature ovarian failure. Therefore, fertility-preserving interventions are required for benign gynecologic diseases. However, there are still little data on the time period required for recovery of ovarian reserve after the laparoscopic unilateral ovarian cystectomy, which is very important for the individualization of treatment protocols. This study aimed at investigating the time needed for the ovarian reserve to recover after laparoscopic unilateral ovarian non-endometriotic cystectomy.

Materials And Methods: Sixty-seven patients with unilateral ovarian non-endometriotic cyst from Zhoupu and Punan Hospitals who underwent laparoscopic unilateral ovarian cystectomy were recruited as a postoperative observation group (POG). Also, 69 healthy age-matched women without ovarian cyst who did not undergo surgery were recruited as a referent group (RFG). Ovarian reserve with the serum anti-Müllerian hormone (AMH), follicle-stimulating hormone (FSH), estradiol (E2) levels, ovarian arterial resistance index (OARI), and antral follicle counts (AFCs) were measured on the third to fifth days of the same menstrual cycle. A postoperative 6-month follow-up of cases was performed.

Results: Compared with RFG, AFC of cyst side in the POG group showed no difference in the first, third, and sixth postoperative month (F = 0.03, F = 0.02, F = 0.55, respectively; p = 0.873, p = 0.878, p = 0.460, respectively). The OARI of cyst side in the POG group revealed no differences in the first, third, and sixth postoperative month (F = 0.73, F = 3.57, F = 1.75, respectively; p = 0.395, p = 0.061, p = 0.701, respectively). In the first month, the postoperative AMH levels significantly declined, reaching 1.88 ng/ml [interquartile range (IQR): 1.61-2.16 ng/ml] in POG and 2.57 ng/ml (IQR: 2.32-2.83 ng/ml) in RFG (F = 13.43, p = 0.000). For the data of AMH levels stratified by age, the same trend was observed between less than 25 and more than 26 years old. At this same time interval, the postoperative rate of decline was significantly lower compared to the preoperative one in POG (32.75%). The same trend was observed between the POG and RFG groups (26.67%).

Conclusions: The optimal time for recovery of ovarian reserve after laparoscopic unilateral ovarian cystectomy is estimated to be 6 months.
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http://dx.doi.org/10.3389/fendo.2021.671225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8498098PMC
September 2021

mmBCFA C17iso ensures endoplasmic reticulum integrity for lipid droplet growth.

J Cell Biol 2021 Nov 8;220(11). Epub 2021 Oct 8.

Center for Life Sciences, School of Life Sciences, Yunnan University, Kunming, Yunnan, China.

In eukaryote cells, lipid droplets (LDs) are key intracellular organelles that dynamically regulate cellular energy homeostasis. LDs originate from the ER and continuously contact the ER during their growth. How the ER affects LD growth is largely unknown. Here, we show that RNAi knockdown of acs-1, encoding an acyl-CoA synthetase required for the biosynthesis of monomethyl branched-chain fatty acids C15iso and C17iso, remarkably prevented LD growth in Caenorhabditis elegans. Dietary C17iso, or complex lipids with C17iso including phosphatidylcholine, phosphatidylethanolamine, and triacylglycerol, could fully restore the LD growth in the acs-1RNAi worms. Mechanistically, C17iso may incorporate into phospholipids to ensure the membrane integrity of the ER so as to maintain the function of ER-resident enzymes such as SCD/stearoyl-CoA desaturase and DGAT2/diacylglycerol acyltransferase for appropriate lipid synthesis and LD growth. Collectively, our work uncovers a unique fatty acid, C17iso, as the side chain of phospholipids for determining the ER homeostasis for LD growth in an intact organism, C. elegans.
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http://dx.doi.org/10.1083/jcb.202102122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563294PMC
November 2021

A randomized, double-blind, single-dose, single-center, parallel phase I clinical study comparing the pharmacokinetics, immunogenicity, safety, and tolerance of pertuzumab injection and Perjeta® in healthy Chinese male subjects.

Expert Opin Biol Ther 2021 Oct 18:1-9. Epub 2021 Oct 18.

Phase I Clinical Trial Laboratory, Affiliated Hospital of Changchun University of Chinese Medicine, Jilin, China.

Background: Perjeta® is a recombinant, humanized monoclonal antibody that has been marketed and approved for the targeted therapy of human epidermal growth factor receptor (HER2) positive breast cancer in the United States. This study compared the bioequivalence, immunogenicity, and safety of pertuzumab injection (a biosimilar of Perjeta® produced by Chia Tai Tianqing Pharmaceutical Group Co., Ltd) and Perjeta® (produced by Roche Pharma AG) in healthy Chinese males.

Research Design And Methods: Healthy Chinese male subjects (N = 87) were randomly given intravenous injection of 5 mg/kg pertuzumab or Perjeta® at a 1:1 ratio. Plasma drug concentrations were detected by enzyme-linked immunosorbent assay, and primary pharmacokinetic parameters were statistically analyzed. We detected the levels of anti-drug antibody (ADA) and neutralizing antibody (nAb) to evaluate drug immunogenicity and safety of the drugs throughout the study.

Results: The geometric mean ratios of AUC, C, and AUC for pertuzumab and Perjeta® were 100.42%, 96.71%, and 101.47%, respectively. The 90% CIs were all within 80%-125%, meeting the bioequivalence standards. The levels of ADA and nAb were similar. In addition, both had good safety in the study.

Conclusion: The study shows that pertuzumab injection and Perjeta® had similar bioequivalence, immunogenicity, and safety.
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http://dx.doi.org/10.1080/14712598.2021.1988567DOI Listing
October 2021

The protective mechanism of a debranched corn starch/konjac glucomannan composite against dyslipidemia and gut microbiota in high-fat-diet induced type 2 diabetes.

Food Funct 2021 Oct 4;12(19):9273-9285. Epub 2021 Oct 4.

State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China.

This study aimed to explore the protection mechanism of a debranched corn starch/konjac glucomannan (DCSK) composite against type 2 diabetes (T2D) related to dyslipidemia and gut microbiota in mice fed on a high-fat diet (HFD). The results showed that the consumption of DCSK led to a significant improvement in the biochemical parameters and physiological indices associated with T2D in the HFD group, including the decrease in blood glucose, triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels, as well as the suppression of the oxidative stress of the liver and kidneys. Furthermore, the health of the intestinal microbiota in the HFD-fed mice was altered dramatically after DCSK consumption. Metabolomics revealed 13 differential metabolites strongly linked to DCSK intervention, and DCSK supplementation regulated amino acid metabolism, nucleotide metabolism, and lipid metabolism. These findings demonstrated that DCSK has an outstanding ability to improve hyperglycemia, hyperlipidemia, and gut microbiota associated with T2D.
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http://dx.doi.org/10.1039/d1fo01233aDOI Listing
October 2021

AKR1C1 alleviates LPS‑induced ALI in mice by activating the JAK2/STAT3 signaling pathway.

Mol Med Rep 2021 Dec 30;24(6). Epub 2021 Sep 30.

Emergency Medicine Department, The First People's Hospital of Lianyungang; 3Emergency Medicine Department, The First Affiliated Hospital of Kangda College of Nanjing Medical University, Lianyungang, Jiangsu 222002, P.R. China.

Acute lung injury (ALI) is a respiratory tract disease characterized by increased alveolar/capillary permeability, lung inflammation and structural damage to lung tissues, which can progress and transform into acute respiratory distress syndrome (ARDS). Although there are several treatment strategies available to manage this condition, there is still no specific cure for ALI. Aldo‑keto reductase family 1 member C1 (AKR1C1) is a member of the aldo‑keto reductase superfamily, and is a well‑known Nrf2 target gene and an oxidative stress gene. The aim of the present study was to investigate the effects of AKR1C1 on a lipopolysaccharide (LPS)‑induced ALI model. After mice received LPS treatment, the mRNA expression levels of AKR1C1 in the bronchoalveolar lavage fluid and serum were measured using reverse transcription‑quantitative PCR and its relationship with the inflammatory factors and malondialdehyde levels were determined using correlation analysis. Next, AKR1C1 was overexpressed or knocked out in mice, and subsequently ALI was induced in mice using LPS. The severity of ALI, oxidative stress and inflammation in the lungs were measured, and the potential involvement of the Janus kinase 2 (JAK2)/signal transduction activator of transcription 3 (STAT3) signaling pathway was assessed by measuring the changes of lung injury parameters after the agonists of JAK2/STAT3 pathway, including interleukin (IL)‑6 and colivelin, were administrated to mice. The results revealed that AKR1C1 expression was decreased in the LPS‑induced ALI mouse model. AKR1C1 expression was inversely correlated with serum tumor necrosis factor‑α, IL‑6 and malondialdehyde levels, and positively correlated with serum IL‑10 levels. AKR1C1 overexpression significantly attenuated lung injury, as shown by the changes in Evans blue leakage in the lung, lung wet/dry weight ratio, PaO/FIO ratio, survival rate of mice and histological lung changes. In addition, the JAK2/STAT3 signaling pathway was significantly deactivated by AKR1C1. When AKR1C1 mice were treated with JAK2/STAT3 agonists, the effects of AKR1C1 overexpression on lung injury and oxidative stress were abolished. In conclusion, AKR1C1 may protect against oxidative stress and serve as a negative regulator of inflammation in ALI/ARDS. In addition, the JAK2/STAT3 signaling pathway could participate in the protective effects of AKR1C1 against ALI.
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http://dx.doi.org/10.3892/mmr.2021.12473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8503743PMC
December 2021

Molecular Characterizations and Functional Analyses of LmR2D2 in the siRNA Pathway.

Insects 2021 Sep 10;12(9). Epub 2021 Sep 10.

Institute of Applied Biology, Shanxi University, Taiyuan 030006, China.

Small interfering RNAs (siRNAs) are non-coding RNAs with a length of 21~23 nucleotides (nt) and present in almost all eukaryotes. The formation of siRNA is a highly conserved post-transcriptional gene-silencing mechanism mediated by key proteins, including Dicer2, Argonaute2 (Ago2) and R2D2. R2D2 has been identified as a double-stranded RNA (dsRNA)-binding protein and reported as an integral component of the siRNA pathway in . However, the involvement of R2D2 in the siRNA pathway of is still unknown. In the present study, we identified an gene from the transcriptome of . It consists of a 954-bp open reading frame that encodes a protein of 318 amino acid residues. Further sequence analysis revealed that LmR2D2 possesses two tandem dsRNA-binding domains (dsRBD) at the N-terminus. Analysis of the developmental expression profile of indicated that its transcript level was stable in third-instar nymphs of , whereas the tissue-dependent expression profile exhibited high levels of expression of in the testis and ovary. When was silenced by RNAi, the RNAi efficiency against as a marker gene was significantly diminished, as indicated by the 37.7% increased transcript level. Additionally, the prokaryotic expression system was used to obtain the LmR2D2 supernatant protein. By incubating the LmR2D2 protein with biotin-dsRNA, we found that LmR2D2 can bind to dsRNA in vitro, which supports our conclusion that LmR2D2 plays an essential role in the siRNA pathway of .
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http://dx.doi.org/10.3390/insects12090812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468669PMC
September 2021

β-1,3-d-Glucan based yeast cell wall system loaded emodin with dual-targeting layers for ulcerative colitis treatment.

Carbohydr Polym 2021 Dec 27;273:118612. Epub 2021 Aug 27.

State Key Laboratory of Southwestern Chinese Medicine Resources, Pharmacy School, Chengdu University of Traditional Chinese Medicine, Chengdu 611130, China. Electronic address:

Herein, a β-1,3-d-glucan based microcarrier, yeast cell wall microparticles (YPs), was used to develop a food-source-based nano-in-micro oral delivery system for ulcerative colitis (UC) treatment. Briefly, lactoferrin (Lf), which targets intestinal epithelial cells, was used to encapsulate emodin (EMO) to form nanoparticles (EMO-NPs), and then loaded into YPs with the natural macrophages targeting ability, forming a final formula with two outer-inner targeting layers (EMO-NYPs). These dual-targeting strategy could enhance the dual-effects of EMO in anti-inflammatory and mucosal repair effects respectively. As expected, cell uptake assessment confirmed that EMO-NPs and EMO-NYPs could target on the Lf and dection-1 receptors on the membranes of Caco-2 cells and macrophages, respectively. Importantly, EMO-NYPs showed the best anti-UC effects compared to EMO-NPs and free EMO, by inhibiting NF-κB pathway to anti-inflammation and promoting intestinal mucosa repair via MLCK/pMLC2 pathway. The results show that EMO-NYPs are a promising food-based oral delivery system in anti-UC.
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http://dx.doi.org/10.1016/j.carbpol.2021.118612DOI Listing
December 2021

Teacher Suggestion Feedback Facilitates Creativity of Students in STEAM Education.

Front Psychol 2021 1;12:723171. Epub 2021 Sep 1.

Shanghai Hui Ye (Lan Zhou) Law Office, Lanzhou, China.

This study examined the influence of the formative feedback types of teachers on creativity in Science, Technology, Engineering, Art, Mathematics (STEAM) education. Participants were 90 undergraduate students who were randomly assigned to the teacher opinions feedback group, the teacher suggestion feedback group, or the non-feedback group, and took part in three courses of STEAM education of 3D-printing technology. Before and after each course, they were asked to fill out the Eugene Creativity Test. The results showed that compare with the teacher opinions feedback group and the non-feedback group, the participants in the teacher suggestion feedback group showed a higher score on the creativity scale. This suggests that the teacher suggestion feedback can be useful for improving the creativity in STEAM education.
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http://dx.doi.org/10.3389/fpsyg.2021.723171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8441010PMC
September 2021

Hypoxia regulates overall mRNA homeostasis by inducing Met-linked linear ubiquitination of AGO2 in cancer cells.

Nat Commun 2021 09 13;12(1):5416. Epub 2021 Sep 13.

State Key Laboratory of Oncogenes and Related Genes, Department of Biochemistry and Molecular Cell Biology & Shanghai Key Laboratory of Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.

Hypoxia is the most prominent feature in human solid tumors and induces activation of hypoxia-inducible factors and their downstream genes to promote cancer progression. However, whether and how hypoxia regulates overall mRNA homeostasis is unclear. Here we show that hypoxia inhibits global-mRNA decay in cancer cells. Mechanistically, hypoxia induces the interaction of AGO2 with LUBAC, the linear ubiquitin chain assembly complex, which co-localizes with miRNA-induced silencing complex and in turn catalyzes AGO2 occurring Met-linked linear ubiquitination (M1-Ubi). A series of biochemical experiments reveal that M1-Ubi of AGO2 restrains miRNA-mediated gene silencing. Moreover, combination analyses of the AGO2-associated mRNA transcriptome by RIP-Seq and the mRNA transcriptome by RNA-Seq confirm that AGO2 M1-Ubi interferes miRNA-targeted mRNA recruiting to AGO2, and thereby facilitates accumulation of global mRNAs. By this mechanism, short-term hypoxia may protect overall mRNAs and enhances stress tolerance, whereas long-term hypoxia in tumor cells results in seriously changing the entire gene expression profile to drive cell malignant evolution.
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http://dx.doi.org/10.1038/s41467-021-25739-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438024PMC
September 2021

Correlation Analysis between Mechanical Power and Lung Ultrasound Score and Their Evaluation of Severity and Prognosis in ARDS Patients.

Biomed Res Int 2021 1;2021:4156162. Epub 2021 Sep 1.

Department of Emergency Medicine, Lianyungang Clinical College of Nanjing Medical University, the First People's Hospital of Lianyungang, Lianyungang, China.

Methods: A total of 121 patients with moderate to severe ARDS admitted to the intensive care unit (ICU) from June 2017 to April 2020 and treated with invasive mechanical ventilation were sequentially included in this study. Their general information was collected, and MP was recorded at 0 h, 24 h, 48 h, and 72 h after admission to the ICU. Professionally trained researchers performed the LUS assessments. Patients were divided into the death and survival groups according to their 28-day prognosis. The trend of MP and LUS at the four time points was analyzed. A receiver operating characteristic curve (ROC) was used to analyze the predictive value of MP and LUS scores at 0 h and 72 h for the prognosis (28-day mortality rate) of patients with moderate to severe ARDS.

Results: 121 patients were included in the analysis, of which 73 were male and 48 were female. When patients entered the ICU, their oxygenation index (: 30885, < 0.01), APACHE II score (: 2.105, < 0.05), and SOFA score (: 4.134, < 0.001) were higher in the death group than the survival group. The death group had significantly higher MP and LUS at each time point (0 h, 24 h, 48 h, and 72 h) compared to the survival group (all < 0.05). There was a significant upward trend over time in the MP and LUS of the death group, contrasting to a significant downward trend in the survival group (all < 0.05). The Pearson correlation analysis showed that MP and LUS were significantly positively correlated at each time point ( values: 0 h: 0.3027; 24 h: 0.3705; 48 h: 0.3902; 72 h: 0.5916; all < 0.01). The ROC curves showed that MP and LUS at 72 h were of significant value in predicting the prognosis of ARDS patients, with areas under the curve of 0.866 ± 0.032 and 0.839 ± 0.037, respectively.

Conclusion: There was a significant correlation between the MP and LUS of ARDS patients at four time points from 0 to 72 h, which has a clinical value in evaluating severity and prognosis.
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http://dx.doi.org/10.1155/2021/4156162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429004PMC
September 2021

Clinical outcomes of uterine arterial chemoembolization with drug-eluting beads for advanced-stage or recurrent cervical cancer.

Abdom Radiol (NY) 2021 12 2;46(12):5715-5722. Epub 2021 Sep 2.

Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, No. 1, East Jian She Road, Zhengzhou, 450052, China.

Purpose: To study the clinical efficacy and safety of transarterial chemoembolization with drug-eluting beads (DEB-TACE) among women with advanced-stage or recurrent cervical cancer.

Methods: This retrospective cohort study enrolled women with cervical cancer who were treated by DEB-TACE between April 3, 2017 and July 12, 2021. Inclusion criteria were pathologic diagnosis of cervical cancer, II-IVa period, being aged 18 to 80 years, patient's inclination of treatment with DEB-TACE, and complete clinicopathologic data. Direct medical cost, hospital stay, resection frequency, treatment responses, adverse events, overall survival, and progression-free survival were investigated.

Results: A total of 16 women with cervical cancer were treated by DEB-TACE. DEB-TACE was successfully performed in all patients, with no major complications or adverse events. A total of 10 minor complications were observed in 9 women (56.3%) after the procedure. Seven (43.8%) women experienced mild to moderate post-embolization pain. The tumors decreased 3 and 6 months after the treatment. The frequency of complete response, partial response, stable disease, and progressive disease was 1 (40%), 3 (40%), 12 (15%), and 0 (0%), respectively, resulting in an objective response rate of 25.0% and a disease control rate of 100.0% after 1 month. The median hospital stay was 9.5 days, and the direct medical cost was 5.9 × 10 ¥. The median follow-up time was 4.1 months (interquartile range 2.6-23.7 months). The median overall survival was 19.1 months, and the 1- and 3-year survival rate was 64.9% and 46.4%, respectively.

Conclusion: DEB-TACE with diamminedichloroplatinum-preloaded beads may be an effective and safe treatment for women with advanced-stage or recurrent cervical cancer.
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http://dx.doi.org/10.1007/s00261-021-03267-6DOI Listing
December 2021

Effects of sub-lethal antimicrobial photodynamic therapy mediated by haematoporphyrin monomethyl ether on polymyxin-resistant Escherichia coli clinical isolate.

Photodiagnosis Photodyn Ther 2021 Aug 29;36:102516. Epub 2021 Aug 29.

Department of Pathogenic Microbiology & Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 710061, PR China. Electronic address:

Background And Aim: It is generally believed that bacteria can not develop resistance to antimicrobial photodynamic therapy (aPDT). This work employed a polymyxin-resistant Escherichia coli clinical isolate (E15017) to study whether it could become resistant to aPDT mediated by haematoporphyrin monomethyl ether (HMME) via consecutive photodynamic treatments at sub-lethal condition.

Methods: The sub-lethal and lethal photodynamic treatment conditions for E15017 were determined by colony forming units (CFU) assay. Bacterial cells of E15017 were treated with 20 cycles of repeated sub-lethal HMME-mediated aPDT, and subsequently subjected to aPDT at lethal condition. The antibiotic susceptibility, zeta-potential and membrane integrity of sub-lethal aPDT treated E15017 cells were also investigated.

Results: After 20 cycles of repeated HMME-mediated aPDT treatments at sub-lethal condition, E15017 cells didn't become more resistant to aPDT. Sub-lethal HMME-mediated aPDT decreased the MIC values of E15017 to ceftazidime and polymyxin E by 4 and 2-fold, respectively, and increased the electronegativity of bacterial surface and affected the bacterial membrane integrity.

Conclusions: The results obtained in this study confirmed that antibiotic-resistant bacteria could not develop resistance to aPDT, and HMME-mediated aPDT is an attractive potential treatment for MDR E. coli caused infections.
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http://dx.doi.org/10.1016/j.pdpdt.2021.102516DOI Listing
August 2021

Improvement in the Output Power of Near-Ultraviolet LEDs of p-GaN Nanorods through SiO Nanosphere Mask Lithography with the Dip-Coating Method.

Nanomaterials (Basel) 2021 Aug 5;11(8). Epub 2021 Aug 5.

Jiangsu Ginjoe Semiconductor Co., Ltd., Xuzhou 221300, China.

In this paper, the conditions of the dip-coating method of SiO nanospheres are optimized, and a neatly arranged single-layer SiO array is obtained. On this basis, a "top-down" inductively coupled plasma (ICP) technique is used to etch the p-GaN layer to prepare a periodic triangular nanopore array. After the etching is completed, the compressive stress in the epitaxial wafer sample is released to a certain extent. Then, die processing is performed on the etched LED epitaxial wafer samples. The LED chip with an etching depth of 150 nm has the highest overall luminous efficiency. Under a 100 mA injection current, the light output power (LOP) of the etched 150 nm sample is 23.61% higher than that of the original unetched sample.
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http://dx.doi.org/10.3390/nano11082009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398248PMC
August 2021

Trait self-control and disinhibited eating in COVID-19: The mediating role of perceived mortality threat and negative affect.

Appetite 2021 12 20;167:105660. Epub 2021 Aug 20.

Key Laboratory of Cognition and Personality, Ministry of Education, Faculty of Psychology, Southwest University, Chongqing, China. Electronic address:

As the coronavirus disease 2019 (COVID-19) spreads globally, people are at risk of developing disinhibited eating behaviors. This study aimed to examine whether perceived mortality threat and negative affect mediate the relationship between trait self-control and disinhibited eating during the pandemic. A longitudinal survey was administered to a sample of college students (N = 634) before the outbreak (September 2019, T1), during the mid-term (February 2020, T2), and in the later stage of the pandemic (April 2020, T3). Self-report measures of trait self-control (T1), perceived mortality threat (T2, T3), negative affect (T2, T3), and disinhibited eating (T2, T3) were successively completed. Trait self-control was found to be negatively associated with negative affect, perceived mortality threat, and disinhibited eating during the mid-term and later stage of the pandemic. Disinhibited eating was positively associated with negative affect and perceived mortality threat. The longitudinal mediation results demonstrated that trait self-control (T1) could negatively predict disinhibited eating (T3) through negative affect (T2) rather than through perceived mortality threat. These findings suggest that trait self-control is of great importance in regulating psychological discomfort and disinhibited eating during stressful periods and that negative affect might be the main psychological mechanism underlying the relationship between self-control ability and disinhibited eating.
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http://dx.doi.org/10.1016/j.appet.2021.105660DOI Listing
December 2021

Tongyang Huoxue Decoction (TYHX) Ameliorating Hypoxia/Reoxygenation-Induced Disequilibrium of Calcium Homeostasis and Redox Imbalance via Regulating Mitochondrial Quality Control in Sinoatrial Node Cells.

Oxid Med Cell Longev 2021 10;2021:3154501. Epub 2021 Aug 10.

Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.

Sick sinus syndrome (SSS) is a disease with bradycardia or arrhythmia. The pathological mechanism of SSS is mainly due to the abnormal conduction function of the sinoatrial node (SAN) caused by interstitial lesions or fibrosis of the SAN or surrounding tissues, SAN pacing dysfunction, and SAN impulse conduction accompanied by SAN fibrosis. Tongyang Huoxue Decoction (TYHX) is widely used in SSS treatment and amelioration of SAN fibrosis. It has a variety of active ingredients to regulate the redox balance and mitochondrial quality control. This study mainly discusses the mechanism of TYHX in ameliorating calcium homeostasis disorder and redox imbalance of sinoatrial node cells (SANCs) and clarifies the protective mechanism of TYHX on the activity of SANCs. The activity of SANCs was determined by CCK-8 and the TUNEL method. The levels of apoptosis, ROS, and calcium release were analyzed by flow cytometry and immunofluorescence. The mRNA and protein levels of calcium channel regulatory molecules and mitochondrial quality control-related molecules were detected by real-time quantitative PCR and Western Blot. The level of calcium release was detected by laser confocal. It was found that after H/R treatment, the viability of SANCs decreased significantly, the levels of apoptosis and ROS increased, and the cells showed calcium overload, redox imbalance, and mitochondrial dysfunction. After treatment with TYHX, the cell survival level was improved, calcium overload and oxidative stress were inhibited, and mitochondrial energy metabolism and mitochondrial function were restored. However, after the SANCs were treated with siRNA (si--tubulin), the regulation of TYHX on calcium homeostasis and redox balance was counteracted. These results suggest that -tubulin interacts with the regulation of mitochondrial function and calcium release. TYHX may regulate mitochondrial quality control, maintain calcium homeostasis and redox balance, and protect SANCs through -tubulin. The regulation mechanism of TYHX on mitochondrial quality control may also become a new target for SSS treatment.
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http://dx.doi.org/10.1155/2021/3154501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373484PMC
August 2021

Mild Hypothermia Therapy Lowers the Inflammatory Level and Apoptosis Rate of Myocardial Cells of Rats with Myocardial Ischemia-Reperfusion Injury via the NLRP3 Inflammasome Pathway.

Comput Math Methods Med 2021 9;2021:6415275. Epub 2021 Aug 9.

The Sixth Department of Cardiology, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China.

Objective: To explore the protective effects and mechanism of mild hypothermia treatment in the treatment of myocardial ischemia-reperfusion injury. . A total of 20 Sprague-Dawley (SD) rats were assigned to 4 groups: the blank control group, sham operation group, ischemia reperfusion group, and mild hypothermia therapy group (each = 5). Some indexes were detected. In addition, myocardial cell models of oxygen-glucose deprivation/reoxygenation injury (OGD) were established. The expression of mRNA IL-6 and TNF- and the key enzyme levels of apoptosis (cleaved-Caspase-3) and the NLRP3 inflammasome/p53 signaling pathway in the models were determined.

Results: The expression of serum IL-6 and TNF- in the mild hypothermia therapy group was significantly lower than that in the ischemia reperfusion group. The mild hypothermia therapy group also showed a significantly lower TUNEL cell count and NLRP3 and p53 phosphorylation levels than the ischemia reperfusion group (all < 0.05). The in vitro mild hypothermia + OGD group also showed significantly lower mRNA expression of IL-6 and TNF- and levels of cleaved Caspase-3, NLRP3, and phosphorylated p53 protein than the OGD group (all < 0.05).

Conclusion: In conclusion, mild hypothermia therapy can inhibit the apoptosis and myocardial inflammation of cells induced by MI/R injury in rats and inhibiting the activity of the NLRP3 inflammasome pathway and p53 signaling pathway may be the mechanism.
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http://dx.doi.org/10.1155/2021/6415275DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371626PMC
November 2021

Comparison of High-flow Nasal Cannula (HFNC) and Conventional Oxygen Therapy in Obese Patients Undergoing Cardiac Surgery: A Systematic Review and Meta-analysis.

In Vivo 2021 Sep-Oct;35(5):2521-2529

Zhaoyuan Renmin Hospital, Yantai, P.R. China;

Background/aim: High-flow nasal cannula (HFNC), a new method for postoperative oxygenation, has increasingly received attention during postoperative care. However, its importance for obese patients undergoing cardiac surgery remains controversial. This systematic review and meta-analysis compared and evaluated HFNC and conventional oxygen therapy (COT) in this patient group.

Materials And Methods: Literature was retrieved by searching eight public databases. Randomized controlled trials (RCTs) were selected. RevMan 5.3 was used to analyze the results and any potential bias. The primary outcome included atelectasis score at 24 h postoperatively. The secondary outcomes included PaO2/FiO2 (ratio), dyspnea score at 24 h postoperatively, intensive care unit (ICU) length of stay, and reintubation.

Results: The search strategy yielded 382 studies after duplicates were removed. Finally, 3 RCTs with a total of 526 patients were included in the present study. Compared with COT, there was no significant difference in atelectasis score, dyspnea score, reintubation, and ICU length of stay.

Conclusion: For obese patients undergoing cardiac surgery, postoperative use of HFNC can maintain patient's oxygenation. Additional clinical studies are needed to investigate the role of HFNC in this patient group.
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http://dx.doi.org/10.21873/invivo.12533DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408737PMC
August 2021

Design and Fabrication of a Sandwichlike Zn/Cu/Al-Zr Coating for Superior Anticorrosive Protection Performance of ZM5 Mg Alloy.

ACS Appl Mater Interfaces 2021 Sep 19;13(34):41120-41130. Epub 2021 Aug 19.

College of Nuclear Science and Technology, Fundamental Science on Nuclear Safety and Simulation Technology Laboratory, Harbin Engineering University, Harbin 150001, P. R. China.

A new three-layered film was fabricated on magnesium (Mg) alloy via electroplating to guard against corrosion in a chloride aqueous environment, which consisted of an underlying double-layered zinc/copper (Zn/Cu) and a top aluminum-zirconium (Al-Zr) layer. The Zn/Cu underlayers not only impeded the galvanic corrosion between the Al-Zr coating and Mg alloy but also improved the adhesive ability between the substrate and the upper Al-Zr layer. Herein, we discussed the nucleus sizes of Al-Zr coatings at the stage of nucleation carried out with different contents of ZrCl in AlCl-1-butyl-3-methylimidazolium chloride ionic liquid. The sandwichlike three-layered Zn/Cu/Al-Zr coatings were systematically investigated by surface morphology, phase structure, hardness, anticorrosion performances, and first-principles calculations. The corrosion current density declined from 1.461 × 10 A·cm of bare Mg to 4.140 × 10 A·cm of the Zn/Cu/Al-Zr3 sample. Neutral salt spray testing demonstrated that the Zn/Cu/Al-Zr3 sample showed no evident signs of corrosion after 6 days of exposure. The enhancement of the corrosion protection property was related to the fact that the application of the Cu layer changed the corrosion direction from initial longitudinal corrosion to extended lateral corrosion and the top Al-Zr coating hindered the transmission of aggressive ions. In addition, upon increasing the Zr content in the alloy films, the Fermi energy reduced initially and then increased. The Al-Zr3 alloy with 8.3 atom % Zr showed the lowest Fermi energy (-3.0823 eV), which exhibited the most efficient corrosion protection. These results showed that the prepared three-layered coating provided reliable corrosion protection to Mg alloy and may thus promote its practical applications.
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http://dx.doi.org/10.1021/acsami.1c11920DOI Listing
September 2021
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