Publications by authors named "Yanjun Liu"

282 Publications

Glutaredoxins and thioredoxin peroxidase involved in defense of emamectin benzoate induced oxidative stress in Grapholita molesta.

Pestic Biochem Physiol 2021 Jul 24;176:104881. Epub 2021 May 24.

Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, China. Electronic address:

Glutaredoxins (Grxs) and thioredoxin peroxidases (Tpxs) are major antioxidant enzyme families involved in regulating cellular redox homeostasis and in defense of enhanced oxidative stress through scavenging reactive oxygen species (ROS). However, the functions of these enzymes have not been reported in the oriental fruit moth, Grapholita molesta (Busck), a worldwide pest of stone and pome fruits. Here, we identified four new antioxidant genes, GmGrx, GmGrx3, GmGrx5, and GmTpx which were induced by exposure with emamectin benzoate, a commonly used biopesticide for G. molesta control. Other environmental factors (low and high temperatures, Escherichia coli and Metarhizium anisopliae) also significantly induced the expression of these genes. After GmGrx or GmTpx silenced by RNA interference (RNAi), the percentage of larval survival to emamectin benzoate were significantly decreased, demonstrating that GmGrx and GmTpx are involved in protecting G. molesta from stresses induced by emamectin benzoate. Furthermore, silenced GmGrx, GmGrx3, GmGrx5, or GmTpx significantly enhanced the enzymatic activities of superoxide dismutase (SOD) (except GmTpx) and peroxidase (POD), as well as the contents of hydrogen peroxide and metabolites ascorbate. Taken together, our results suggest that GmGrx, GmGrx3, GmGrx5, and GmTpx may play critical roles in antioxidant defense. Specially, GmGrx and GmTpx contribute to the defense of oxidative damage induced by exposure to emamectin benzoate through scavenging excessive ROS in G. molesta. Our findings provided a theoretical basis for understanding functions of insect glutaredoxin and peroxidase systems.
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http://dx.doi.org/10.1016/j.pestbp.2021.104881DOI Listing
July 2021

Automatic Quantification Of Subsurface Defects By Analyzing Laser Ultrasonic Signals Using Convolutional Neural Networks And Wavelet Transform.

IEEE Trans Ultrason Ferroelectr Freq Control 2021 Jun 9;PP. Epub 2021 Jun 9.

The conventional machine learning algorithm for analyzing ultrasonic signals to detect structural defects necessarily identifies and extracts either time or frequency domain features manually, which has problems in reliability and effectiveness. This work proposes a novel approach by combining convolution neural networks (CNN) and wavelet transform to analyze the laser generated ultrasonic signals for detecting the width of subsurface defects accurately. The novelty of this work is to convert the laser ultrasonic signals into the scalograms (images) via wavelet transform, which are subsequently utilized as the image input for the pre-trained CNN to extract the defect features automatically to quantify the width of defects, avoiding the necessity and inaccuracy induced by artificial feature selection. The experimentally validated numerical model that simulates the interaction of laser-generated ultrasonic waves with subsurface defects is firstly established, which is further utilized to generate adequate laser ultrasonic signals for training the CNN model. A total number of 3104 data are obtained from simulation and experiments, with 2480 simulated signals for training the CNN model and the remaining 620 simulated data together with 4 experimental signals for verifying the performance of the proposed algorithm. This approach achieves the prediction accuracy of 98.5% on validation set, particularly with the prediction accuracy of 100% for the 4 experimental data. This work proves the feasibility and reliability of the proposed method for quantifying the width of subsurface defects and can be further expanded as an universal approach to various other defects detection, such as defect locations and shapes.
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http://dx.doi.org/10.1109/TUFFC.2021.3087949DOI Listing
June 2021

Performance comparison of ejectors in ejector-based refrigeration cycles with R1234yf, R1234ze(E) and R134a.

Environ Sci Pollut Res Int 2021 Jun 4. Epub 2021 Jun 4.

School of Energy and Power Engineering, Shandong University, Jinan, 266237, China.

To look for new refrigerants replacing R134a, environmentally friendly refrigerants R1234yf and R1234ze(E) were chosen as the alternatives in an ejector refrigeration system. The tested system contained a single-phase ejector, and numerical analysis regarding the performance of the ejector was done by CFD. The entrainment ratio, static pressure and Mach number were chosen as indicators revealing the ejector performance. Changes of the indicators of ejector utilizing a given refrigerant under varying operating temperature conditions were analysed. With the increasing condenser temperature, the shocking position moved upstream until it combined with the first series of oblique shocks. With the increasing generator temperature, the entrainment ratio increased firstly and decreased subsequently. With the increasing evaporator temperature, the primary-fluid jet expansion weakened. Comparisons of the indicators of ejector utilizing three working fluids (R134a, R1234yf and R1234ze(E)) under a given operating temperature condition were also made. The results showed that R1234yf had a greater entrainment ratio than R134a and R1234ze(E). But the shocking position of R1234yf was also closer to the upstream than the other two refrigerants, which caused the smaller critical mode region. Compared with the entrainment ratio close to 1.4 times, the critical temperature difference of only 3 °C could not weaken the advantages of R1234yf. Besides, heat transfer inside the ejector was considered and not much different trends with the three working fluids were found. Therefore, R1234yf could be the better candidate for R134a in the ejector refrigeration system.
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http://dx.doi.org/10.1007/s11356-021-14626-7DOI Listing
June 2021

Intestinal submucous fibrovascular hamartoma: A case report.

Radiol Case Rep 2021 Jul 23;16(7):1857-1861. Epub 2021 May 23.

Number 155, Nanjing North Street, Heping District, Shenyang City, Liaoning Province, China.

Intestinal submucosal fibrovascular hamartoma is considered as a rare intestinal lesion. We present the case of a 63-year-old female with abdominal symptoms, bleeding, and increased serum tumor markers. The abdominal ultrasound revealed that the left abdominal intestinal wall and mesentery were thickened with enlarged multiple lymph nodes, suggesting intestinal obstruction. Other imaging findings confirmed the ultrasound findings. Histopathology of the removed lesion provided the diagnosis of intestinal submucosal fibrovascular hamartoma with hemorrhage, inflammation, and amyloidosis. Intestinal submucosal fibrovascular hamartoma is a hemorrhagic lesion with macroscopic tumor due to the abnormal mixing of the organ's normal components, which still remains a challenge for clinicians and pathologists. We consider routine abdominal ultrasonography and contrast-enhanced ultrasonography (SICUS) to be safe and effective in the diagnosis of intestinal neoplastic lesions.
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http://dx.doi.org/10.1016/j.radcr.2021.04.048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144528PMC
July 2021

Biomineralized calcium carbonate nanohybrids for mild photothermal heating-enhanced gene therapy.

Biomaterials 2021 Jul 13;274:120885. Epub 2021 May 13.

Key Lab of Biomedical Materials of Natural Macromolecules (Beijing University of Chemical Technology), Ministry of Education, Beijing Laboratory of Biomedical Materials, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, China. Electronic address:

It is of great significance to develop multifunctional gene carriers to achieve treatments with enhanced therapeutic effects in an inflammation-free manner. In this work, assembled micelles of polysaccharide were utilized for the biomineralization of calcium carbonate to produce one-dimensional Alg-CaCO nanoparticles. In order to introduce both functions of mild hyperthermia and gene transfection, polydopamine (PDA) coating was applied to conjugate cationic polymers on the surface of nanoparticles. The resultant ACDP nanohybrids exhibited enhanced performance as gene carriers under near infrared (NIR) light irradiation at a low power density. Meanwhile, the pH-responsive degradation of gene carriers could further promote gene release for better effectiveness. The enhanced gene therapy induces tumor cell apoptosis, which could prevent inflammatory responses. The feasibility of mild hyperthermia-enhanced gene therapy for tumor treatment was investigated in vitro and in vivo. In addition, dual-modal ultrasound (US) and photoacoustic (PA) imaging was also realized to monitor and guide the treatment processes. The current work provides a new avenue for the construction of multifunctional platform to realize cancer therapy with improved therapeutic effectiveness in an inflammation-free manner.
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http://dx.doi.org/10.1016/j.biomaterials.2021.120885DOI Listing
July 2021

Cost-effectiveness analysis of COPD screening programs in primary care for high-risk patients in China.

NPJ Prim Care Respir Med 2021 May 20;31(1):28. Epub 2021 May 20.

National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

We built a decision-analytic model to compare the cost-effectiveness of using portable spirometer and questionnaire to screen chronic obstructive pulmonary diseases (COPD) with no screening (i.e. usual care) among chronic bronchitis patient in China. A lifetime horizon and a payer perspective were adopted. Cost data of health services including spirometry screening and treatment costs covered both maintenance and exacerbation. The result indicated that portable spirometer screening was cost-saving compared with questionnaire screening and no screening, with an incremental cost-effectiveness ratio (ICER) of -5026 and -1766 per QALY, respectively. Sensitivity analyses confirmed the robustness of the results. In summary, portable spirometer screening is likely the optimal option for COPD screening among chronic bronchitis patients China.
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http://dx.doi.org/10.1038/s41533-021-00233-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137942PMC
May 2021

Plasmalogen attenuates the development of hepatic steatosis and cognitive deficit through mechanism involving p75NTR inhibition.

Redox Biol 2021 Jul 7;43:102002. Epub 2021 May 7.

College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, China; Qingdao National Laboratory for Marine Science and Technology, 266235, Qingdao, Shandong Province, China. Electronic address:

Emerging evidence suggests that the reduction of ethanolamine plasmalogen (PlsEtn) is associated with in Alzheimer's disease and metabolic diseases. However, the mechanistic bases for PlsEtn on the these diseases are not well understood. Plasmalogens are primarily synthesized in the liver and enriched in brain. To this end, the present study sought to investigate the potential role of PlsEtn on steatohepatitis and memory impairments and its underlying mechanism. Here we show that peroxisome dysfunction and impairment of PlsEtn synthesis pathway occurs in both of hippocampus and liver, resulting in the decrease of PlsEtn level in APP/PS1 mice and HFD-fed mice. shGNPAT induced PlsEtn deficiency in hepatocytes induces p75NTR enhancement leading to decreased lipolysis activity, thereby exacerbating steatosis. Moreover, in the brain, PlsEtn administration appears to not only improve steatosis but also prevent Alzheimer's disease through restoration of TrkA/p75NTR balance. Together, our findings reveal a molecular mechanistic insight into the preventive role of plasmalogen modulation against steatosis and memory impairments via p75NTR inhibition.
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http://dx.doi.org/10.1016/j.redox.2021.102002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134739PMC
July 2021

A Fluorescence Sensor for Pb Detection Based on Liquid Crystals and Aggregation-Induced Emission Luminogens.

ACS Appl Mater Interfaces 2021 May 9;13(19):22361-22367. Epub 2021 May 9.

Department of Electrical and Electronic Engineering, Southern University of Science and Technology, Shenzhen 518055, China.

Heavy metals, such as lead ions, are regarded as the main environmental contaminants and have a negative impact on human bodies, making detection technologies of lead ions critical. However, most existing detection methods suffer from time consumption, complicated sample pretreatment, and expensive equipment, which hinder their broad use in real-time detection. Herein, we show a new fluorescence sensor for detecting lead ions derived from liquid crystals doped with an aggregation-induced emission luminogen. The mechanism is based on the variation of fluorescence intensity caused by the disturbance of an ordered liquid crystal configuration in the presence of Pb, induced by DNAzyme and its catalytic cleavage. The proposed fluorescence sensor exhibits a low detection limit of 0.65 nM, which is 2 orders of magnitude lower than that previously reported in an optical sensor based on liquid crystals. The detection range of the Pb fluorescence sensor is broad, from 20 nM to 100 μM, and it also selects lead ions from numerous metal ions exactly, resulting in a highly sensitive, highly selective, simple, and low-cost detection strategy of Pb with potential applications in chemical and biological fields. This approach to designing a liquid crystal fluorescence sensor offers an inspiring stage for detecting biomacromolecules or other heavy metal ions by varying decorated molecules.
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http://dx.doi.org/10.1021/acsami.1c02585DOI Listing
May 2021

The effects of multiple controlled ovarian hyperstimulation over a 2-year period on ovarian reserve and reactivity: a retrospective clinical study.

Ann Palliat Med 2021 Apr;10(4):4391-4397

Department of Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.

Background: To evaluate the effects of controlled ovarian hyperstimulation (COH) on ovarian reserve function during in vitro fertilization and embryo transfer (IVF-ET).

Methods: From August 2018 to August 2020, the medical records of patients who received IVF-ET in the Department of Reproductive Medicine, Beijing Gynaecology and Obstetrics Hospital, Capital Medical University were analyzed retrospectively. Among them, 372 patients received 2 cycles of COH, 54 patients received 3 cycles, and 13 patients received 4 cycles. The levels of follicle-stimulating hormone (FSH), the number of antral follicles, levels of anti-Müllerian hormone (AMH), the total amount of gonadotropin (GN), the time of ovulation induction, the number of eggs obtained, the number of available embryos, and the number of high-quality embryos cycles were compared in different treatment.

Results: The age of female patients did not significantly affect the levels of AMH or FSH during menstruation, nor the number of antral follicles before ovulation induction (P>0.05). However, with an increase in age, an increase in the number of controlled COH cycles was observed. In patients who underwent 2 COH cycles, the number of high-quality embryos in the second cycle increased significantly compared to the first cycle (P<0.05). However, there were no significant differences in the ovulation induction time, the number of eggs, the GN dosage, and the number of available embryos (P>0.05). In patients with 3 treatment cycles, the GN dose used in the third cycle was significantly lower than that used in the first cycle (P<0.05). There were no significant differences in the ovulation induction time, the number of eggs obtained, and the quality of embryos (P>0.05). In patients with 4 treatment cycles, significant differences were observed in the ovulation induction time between the first and the fourth controlled COH cycle (P<0.05). However, no significant differences were detected in GN dosage, ovulation induction time, the number of eggs obtained, the number of available embryos, and the quality of embryos (P>0.05).

Conclusions: Ovarian reserve function was not significantly affected in patients with up to 4 ovarian stimulation cycles.
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http://dx.doi.org/10.21037/apm-21-330DOI Listing
April 2021

Ultrasonographic differentiation and Ultrasound-based management of partially cystic thyroid nodules.

Arch Endocrinol Metab 2021 Apr 29. Epub 2021 Apr 29.

Department of Endocrinology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, PR China,

Objective: To determine sonographic features of malignancy in partially cystic thyroid nodules and assess the diagnostic efficacy of these features for differentiating between benign and malignant lesions in the nodules with indeterminate cytology.

Methods: From January 2016 to December 2017, a total of 91 patients with 94 partially cystic thyroid nodules who had undergone ultrasound-guided fine-needle aspiration biopsy and thyroid surgery in our hospital were included in this study. The sonographic features of the thyroid nodules were analyzed to identify the predictive features of malignancy and assess the diagnostic efficacy of these features.

Results: The features of hypoechogenicity, microcalcification, composition, and an eccentric solid component with an acute angle had statistically significant associations with malignant nodule (p<005) by univariable analysis. Binary logistic regression analysis showed that microcalcification and hypoechogenicity were significantly associated with malignancy. Using the combination of microcalcification, hypoechogenicity, and a solid component comprising of greater than or equal to 50% of the total volume, the diagnostic sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy were 97.6%, 32.7%, 53.9%, and 94.4%, respectively. In these nodules with indeterminate cytology, this combination also exhibited a high sensitivity of 92.3% and an NPV of 83.3%.

Conclusion: This study demonstrated that microcalcification and hypoechogenicity were independently associated with malignancy in partially cystic thyroid nodules. The combination of microcalcification, hypoechogenicity, and a solid portion that is greater than or equal to 50% of the total volume will help guide clinical decisions in mixed cystic solid nodules.
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http://dx.doi.org/10.20945/2359-3997000000367DOI Listing
April 2021

Activation of anterior thalamic reticular nucleus GABAergic neurons promotes arousal from propofol anesthesia in mice.

Acta Biochim Biophys Sin (Shanghai) 2021 Apr 30. Epub 2021 Apr 30.

Department of Anesthesiology, Eye and ENT Hospital, Fudan University, Shanghai 200031, China.

Propofol is widely used for the induction and maintenance of anesthesia, which causes a rapid loss of consciousness. However, the mechanisms underlying the hypnosis effect of propofol are still not fully understood. The thalamic reticular nucleus (TRN) is crucial for regulating wakefulness, sleep rhythm generation, and sleep stability, while the role of TRN in the process of propofol-induced anesthesia is still unknown. Here, we investigated the function of the anterior TRN in propofol general anesthesia. Our results demonstrated that the neural activity of anterior TRN is suppressed during propofol anesthesia, whereas it is robustly activated from anesthesia by recording the calcium signals using fiber photometry technology. The results showed that the activation of anterior TRN neurons by chemogenetic and optogenetic methods shortens the emergency time without changing the induction time. Conversely, chemogenetic or optogenetic inhibition of the TRN neurons leads to a delay in the recovery time. Our study showed that anterior TRN is crucial for behavioral arousal without affecting the induction time of propofol anesthesia.
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http://dx.doi.org/10.1093/abbs/gmab056DOI Listing
April 2021

Probiotics Can Further Reduce Waist Circumference in Adults with Morbid Obesity after Bariatric Surgery: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Evid Based Complement Alternat Med 2021 1;2021:5542626. Epub 2021 Apr 1.

The Center of Gastrointestinal and Minimally Invasive Surgery, Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu, Chengdu, China.

Whether probiotics could be used as an adjunct to bariatric surgery is controversial. This meta-analysis aimed to evaluate the effects of probiotics on body weight, body mass index (BMI), percentage of the excess weight loss (%EWL), waist circumference (WC), and C-reactive protein (CRP) in adults with obesity after bariatric surgery (BS). PUBMED, EMBASE, and the Cochrane Central Registry of Controlled Trials were searched from the earliest record to March 2020. All randomized controlled trials (RCTs) on the effects of probiotics in adults with obesity after bariatric surgery were analyzed according to the eligibility criteria. Four RCTs, including 172 participants, were analyzed. There was a statistically significant difference in probiotics in the reduction of waist circumference at 12 months after bariatric surgery. However, probiotics were not effective in weight, BMI, %EWL, WC, and CRP both within 3 months and at 12 months postoperation. Probiotics aid adults with morbid obesity in achieving further waist circumference improvement after BS, with no significant effect on weight, BMI, %EWL, and CRP. More quality clinical studies are needed to confirm the efficacy and safety of probiotics, and address a number of practical issues before the routine clinical use of probiotics in adults with obesity undergoing BS.
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http://dx.doi.org/10.1155/2021/5542626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032506PMC
April 2021

Decreased 11β-Hydroxysteroid Dehydrogenase Type 2 Expression in the Kidney May Contribute to Nicotine/Smoking-Induced Blood Pressure Elevation in Mice.

Hypertension 2021 Jun 5;77(6):1940-1952. Epub 2021 Apr 5.

Department of Pediatrics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, China (Y.W., R.Y., Y.L.).

[Figure: see text].
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.120.16458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119334PMC
June 2021

Cyclin-dependent kinase inhibitor roscovitine attenuates liver inflammation and fibrosis by influencing initiating steps of liver injury.

Clin Sci (Lond) 2021 Apr;135(7):925-941

Department of Gastroenterology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, 1665 Kongjiang Road, Shanghai 200092, China.

Liver diseases present a significant public health burden worldwide. Although the mechanisms of liver diseases are complex, it is generally accepted that inflammation is commonly involved in the pathogenesis. Ongoing inflammatory responses exacerbate liver injury, or even result in fibrosis and cirrhosis. Here we report that roscovitine, a cyclin-dependent kinase (CDK) inhibitor, exerts beneficial effects on acute and chronic liver inflammation as well as fibrosis. Animal models of lipopolysaccharide (LPS)/d-galactosamine- and acute or chronic CCl4-induced liver injury showed that roscovitine administration markedly attenuated liver injury, inflammation and histological damage in LPS/d-galactosamine- and CCl4-induced acute liver injury models, which is consistent with the results in vitro. RNA sequencing (RNA-seq) analysis showed that roscovitine treatment repressed the transcription of a broad set of pro-inflammatory genes involved in many aspects of inflammation, including cytokine production and immune cell proliferation and migration, and inhibited the TGF-β signaling pathway and the biological process of tissue remodeling. For further validation, the beneficial effect of roscovitine against inflammation was evaluated in chronic CCl4-challenged mice. The anti-inflammation effect of roscovitine was observed in this model, accompanied with reduced liver fibrosis. The anti-fibrotic mechanism involved inhibition of profibrotic genes and blocking of hepatic stellate cell (HSC) activation. Our data show that roscovitine administration protects against liver diseases through inhibition of macrophage inflammatory actions and HSC activation at the onset of liver injury.
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http://dx.doi.org/10.1042/CS20201111DOI Listing
April 2021

Robust Standard Gradient Descent Algorithm for ARX Models Using Aitken Acceleration Technique.

IEEE Trans Cybern 2021 Mar 23;PP. Epub 2021 Mar 23.

A robust standard gradient descent (SGD) algorithm for ARX models using the Aitken acceleration method is developed. Considering that the SGD algorithm has slow convergence rates and is sensitive to the step size, a robust and accelerative SGD (RA-SGD) algorithm is derived. This algorithm is based on the Aitken acceleration method, and its convergence rate is improved from linear convergence to at least quadratic convergence in general. Furthermore, the RA-SGD algorithm is always convergent with no limitation of the step size. Both the convergence analysis and the simulation examples demonstrate that the presented algorithm is effective.
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http://dx.doi.org/10.1109/TCYB.2021.3063113DOI Listing
March 2021

Roles of the Dynamic Tumor Immune Microenvironment in the Individualized Treatment of Advanced Clear Cell Renal Cell Carcinoma.

Front Immunol 2021 4;12:653358. Epub 2021 Mar 4.

Department of Urology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Immune checkpoint inhibitors (ICIs) are currently a first-line treatment option for clear cell renal cell carcinoma (ccRCC). However, recent clinical studies have shown that a large number of patients do not respond to ICIs. Moreover, only a few patients achieve a stable and durable response even with combination therapy based on ICIs. Available studies have concluded that the response to immunotherapy and targeted therapy in patients with ccRCC is affected by the tumor immune microenvironment (TIME), which can be manipulated by targeted therapy and tumor genomic characteristics. Therefore, an in-depth understanding of the dynamic nature of the TIME is important for improving the efficacy of immunotherapy or combination therapy in patients with advanced ccRCC. Here, we explore the possible mechanisms by which the TIME affects the efficacy of immunotherapy and targeted therapy, as well as the factors that drive dynamic changes in the TIME in ccRCC, including the immunomodulatory effect of targeted therapy and genomic changes. We also describe the progress on novel therapeutic modalities for advanced ccRCC based on the TIME. Overall, this review provides valuable information on the optimization of combination therapy and development of individualized therapy for advanced ccRCC.
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http://dx.doi.org/10.3389/fimmu.2021.653358DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970116PMC
March 2021

Clinical Significance of a Novel Tumor Progression-Associated Immune Signature in Colorectal Adenocarcinoma.

Front Cell Dev Biol 2021 25;9:625212. Epub 2021 Feb 25.

The Center of Gastrointestinal and Minimally Invasive Surgery, Department of General Surgery, The Third People's Hospital of Chengdu, Chengdu, China.

Background: Some colorectal adenocarcinoma (CRC) patients are susceptible to recurrence, and they rapidly progress to advanced cancer stages and have a poor prognosis. There is an urgent need for efficient screening criteria to identify patients who tend to relapse in order to treat them earlier and more systematically.

Methods: We identified two groups of patients with significantly different outcomes by unsupervised cluster analysis of GSE39582 based on 101 significantly differentially expressed immune genes. To develop an accurate and specific signature based on immune-related genes to predict the recurrence of CRC, a multivariate Cox risk regression model was constructed with a training cohort composed of 519 CRC samples. The model was then validated using 129, 292, and 446 samples in the real-time quantitative reverse transcription PCR (qRT-PCR), test, and validation cohorts, respectively.

Results: This classification system can also be used to predict the prognosis in clinical subgroups and patients with different mutation states. Four independent datasets, including qRT-PCR and The Cancer Genome Atlas (TCGA), demonstrated that they can also be used to accurately predict the overall survival of CRC patients. Further analysis suggested that high-risk patients were characterized by worse effects of chemotherapy and immunotherapy, as well as lower immune scores. Ultimately, the signature was identified as an independent prognostic factor.

Conclusion: The signature can accurately predict recurrence and overall survival in patients with CRC and may serve as a powerful prognostic tool to further optimize cancer immunotherapy.
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http://dx.doi.org/10.3389/fcell.2021.625212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959763PMC
February 2021

High expression of CD52 in adipocytes: a potential therapeutic target for obesity with type 2 diabetes.

Aging (Albany NY) 2021 03 11;13(8):11043-11060. Epub 2021 Mar 11.

The Center of Gastrointestinal and Minimally Invasive Surgery, The Third People's Hospital of Chengdu, Affiliated Hospital of Southwest Jiaotong University, Chengdu 610031, China.

The aim of the present study was to evaluate the involvement of CD52 in adipocytes as well as to explore its effect on type 2 diabetes mellitus (T2DM), and to improve our understanding of the potential molecular events of obesity with type 2 diabetes. Global changes in the CD52 expression patterns were detected in adipocytes and preadipocytes derived from obese and lean individuals. In particular, CD52 was identified as significantly differentially upregulated and was analyzed, both and , using various approaches. experiments, CD52 was a significantly up-regulated mRNA in mature adipocytes and preadipocytes. In addition, CD52 gradually increased with the differentiation of preadipocytes. experiments, the expression of CD52 in high-fat diet (HFD) -fed mice tended to be higher than that in regular diet (RD) -fed mice. Further analysis showed that CD52 expression was positively correlated with Smad3 and TGF-β in mice, and the downregulation of CD52 was accompanied by increased glucose tolerance and insulin sensitivity. Moreover, a comparison of CD4+CD52 T cells and CD4+CD52 T cells showed that many T2DM-related genes were aberrantly expressed. Overall, CD52 may functioned as an important potential target for obesity with T2DM via TGF-β/Smad3 axis.
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http://dx.doi.org/10.18632/aging.202714DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8109061PMC
March 2021

Discovery and characterization of a novel glucose-6-phosphate dehydrogenase (G6PD) inhibitor via high-throughput screening.

Bioorg Med Chem Lett 2021 May 6;40:127905. Epub 2021 Mar 6.

School of Chinese Materia Medica, Nanjing University of Chinese Medicine, 138 Xianlin Road, Qixia, Nanjing 210023, Jiangsu, China; The Center for Chemical Biology, Drug Discovery and Design Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Road, Shanghai 201203, China; School of Pharmaceutical Science and Technology, Hangzhou Institute for Advanced Study, UCAS, Hangzhou 310024, China. Electronic address:

Altered glucose-6-phosphate dehydrogenase (G6PD) status is influential in many cellular pathophysiological processes and diseases, making G6PD a potential target for cancer therapy. However, the available G6PD inhibitors are very limited and restricted. Here we developed a reducing equivalent nicotinamide adenine dinucleotide phosphate (NADPH) absorption photometry assay based on enzyme kinetics to characterize G6PD activity. In this way, we performed a high-throughput screening (HTS) to an in house library. And then we identified compound named Wedelolactone inhibiting G6PD strongly in a non-competitive, reversible way. In addition, we did the surface Plasmon Resonance (SPR) assay and indicated the K between Wedelolactone and G6PD protein was 3.64 μM. Furthermore, our basic colony formation assay showed the inhibitory effect of Wedelolactone on the proliferation of ovarian cancer cells (IC ~ 10 µM). Thus, we provided a high-throughput screening assay to quickly and efficiently discover G6PD inhibitors, and identified Wedelolactone as a G6PD inhibitor, implying that Wedelolactone suppresses ovarian cancer partly through targeting G6PD.
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http://dx.doi.org/10.1016/j.bmcl.2021.127905DOI Listing
May 2021

Controlled Synthesis and Surface Engineering of Janus Chitosan-Gold Nanoparticles for Photoacoustic Imaging-Guided Synergistic Gene/Photothermal Therapy.

Small 2021 Mar 23;17(11):e2006004. Epub 2021 Feb 23.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.

The unsymmetrical morphology and unique properties of Janus nanoparticles (JNPs) provide superior performances for biomedical applications. In this work, a general and facile strategy is developed to construct a series of symmetrical and unsymmetrical chitosan/gold nanoparticles. Taking advantage of the active motion derived from Janus structure, selective surface functionalization of polysaccharide domain, and photothermal effect of gold nanorods, Janus chitosan/gold nanoparticles (J-Au-CS) are selected as a model system to construct Janus-structured chitosan/gold nanohybrids (J-ACP). Near-infrared (NIR)-responsive J-ACP composed of polycationic chitosan nanospheres and PEGylated gold nanorods hold great potential to realize photoacoustic (PA) imaging-guided complementary photothermal therapy (PTT)/gene therapy for breast cancer. The morphology effect of chitosan/gold nanostructures on enhanced PTT, cellular uptake, and gene transfection is investigated. The feasibility of PA imaging to track the accumulation of J-ACP and guide PTT is also explored. Notably, synergistic therapy is achieved based on PTT-enhanced gene therapy. In addition, the loading function of chitosan/gold nanoparticles for fluorescence imaging is demonstrated. The current work extends the application of JNPs for imaging-guided synergistic cancer therapy and provides flexible candidates with distinct structures for diverse biomedical applications.
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http://dx.doi.org/10.1002/smll.202006004DOI Listing
March 2021

Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways.

Biosci Biotechnol Biochem 2021 Feb;85(3):675-686

College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong, People's Republic of China.

Gangliosides (GLSs) are ubiquitously distributed in all tissues but highly enriched in nervous system. Currently, it is unclear how exogenous GLSs regulate neuritogenesis, although neural functions of endogenous GLSs are widely studied. Herein, we evaluated the neuritogenic activities and mechanism of sea urchin gangliosides (SU-GLSs) in vitro. These different glycosylated SU-GLSs, including GM4(1S), GD4(1S), GD4(2A), and GD4(2G), promoted differentiation of NGF-induced PC12 cells in a dose-dependent and structure-selective manner. Sulfate-type and disialo-type GLSs exhibited stronger neuritogenic effects than monosialoganglioside GM1. Furthermore, SU-GLSs might act as neurotrophic factors possessing neuritogenic effects, via targeting tyrosine-kinase receptors (TrkA and TrkB) and activating MEK1/2-ERK1/2-CREB and PI3K-Akt-CREB pathways. This activation resulted in increased expression and secretion of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). These pathways were verified by specific inhibitors. Our results confirmed the neuritogenic functions of SU-GLS in vitro and indicated their potential roles as natural nutrition for neuritogenesis.
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http://dx.doi.org/10.1093/bbb/zbaa088DOI Listing
February 2021

Improvement of left ventricular systolic function in morbidly obese patients after bariatric surgery: Case report.

Medicine (Baltimore) 2021 Feb;100(6):e24309

Affiliated to Southwest Jiaotong University Obesity and Metabolism Center, Third People's Hospital of Chengdu.

Introduction: Morbid obesity (body mass index > 40 kg/m2) is a risk factor for the development of left ventricular systolic dysfunction (LVSD) and can complicate the management of LVSD. Bariatric surgery is increasingly recognized as a safe and effective way to achieve marked weight loss, but studies on improving LVSD populations are limited. We retrospectively analyzed the first case of the Asia-Pacific region with morbid obesity and left ventricular ejection fraction (LVEF) < 50% who underwent bariatric surgery at our medical center.

Patient Concerns: The patient was admitted to the hospital due to progressive weight gain for more than 10 years. The patient used to be in good health. One year before admission, the patient was hospitalized in another hospital due to shortness of breath. After the relevant examination, the patient was diagnosed with dilated cardiomyopathy.

Diagnosis: The body mass index of the patient was 45.9 kg/m2, and the patient was diagnosed with morbid obesity. He was diagnosed with dilated cardiomyopathy and cardiac function class IV in another hospital. After completing a preoperative examination, the patient was diagnosed with hyperuricemia, hyperlipidemia, fatty liver disease and severe sleep apnea.

Interventions: The patient successfully underwent laparoscopic sleeve gastrectomy plus jejunal bypass.

Outcomes: Six months after the surgery, patient weight lost was 33.6 kg, and the LVEF increased from 31% to 55%. The cardiac function of the patient recovered from class IV to class I, and the patient's hyperuricemia, hyperlipidemia and sleep apnea were significantly improved.

Conclusion: Bariatric surgery may be a safe and effective intervention for morbidly obese patients with LVSD. Bariatric surgery was associated with an improvement in LVEF. However, the specific mechanism still needs further study.
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http://dx.doi.org/10.1097/MD.0000000000024309DOI Listing
February 2021

Quadrangular resection versus chordal replacement for degenerative posterior mitral leaflet prolapse.

Ann Transl Med 2021 Jan;9(1):60

Department of Cardiovascular Surgery, Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Guangzhou, China.

Background: The aims of the present study was to compare midterm results of quadrangular leaflet resection versus chordal replacement for the repair of degenerative posterior mitral leaflet (PML) prolapse, and to explore the risk factors for recurrent severe mitral regurgitation (MR).

Methods: From January 2012 to December 2018, 1,423 consecutive patients underwent mitral valve (MV) repair. A total of 317 had degenerative PML prolapse and constituted the study population. Of these, 74 (23.3%) underwent quadrangular leaflet resection, and 243 (76.7%) underwent chordal replacement. Outcomes were compared by using unadjusted data and propensity score-matched analyses.

Results: Patients with multiple leaflet prolapse were more likely to undergo chordal replacement (18.4% 41.9%, P<0.001), and performed as a minimally invasive approach (47.3% 61.7%, P=0.027). Of the entire cohort, 1 death (0.3%) occurred due to intraoperative aortic dissection, and 1 patient who had undergone chordal replacement required reoperation before discharge for posterior leaflet tearing. There was no significant difference in the probability of freedom from recurrent severe MR at 82 months between the resection and neochordae groups in both the pre-matched (95.6% 88.8%, P=0.105) and matched (95.2% 88.5%, P=0.170) cohorts, which was consistent across all of the examined subgroups (P>0.05). Multivariate Cox regression indicated that dilated left ventricular end-systolic diameter (LVESD) was an independent risk factor for recurrent severe MR [<40 >40 mm, hazards ratio (HR): 3.17, 95% confidence interval (CI): 1.20-8.39, P=0.020]; however, surgical technique was not (resection neochordae, HR: 0.31, 95% CI: 0.07-1.37, P=0.122).

Conclusions: Chordal replacement for the repair of degenerative posterior MV prolapse yields similar satisfactory outcomes when compared with quadrangular resection, and is promising in minimally invasive cardiac surgery for various lesions. However, it is also associated with more recurrent severe MR, albeit non-significant, for which patients with dilated LVESD are at high risk.
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http://dx.doi.org/10.21037/atm-20-7475DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859790PMC
January 2021

Observer-Based Neuro-Adaptive Optimized Control of Strict-Feedback Nonlinear Systems With State Constraints.

IEEE Trans Neural Netw Learn Syst 2021 Jan 26;PP. Epub 2021 Jan 26.

This article proposes an adaptive neural network (NN) output feedback optimized control design for a class of strict-feedback nonlinear systems that contain unknown internal dynamics and the states that are immeasurable and constrained within some predefined compact sets. NNs are used to approximate the unknown internal dynamics, and an adaptive NN state observer is developed to estimate the immeasurable states. By constructing a barrier type of optimal cost functions for subsystems and employing an observer and the actor-critic architecture, the virtual and actual optimal controllers are developed under the framework of backstepping technique. In addition to ensuring the boundedness of all closed-loop signals, the proposed strategy can also guarantee that system states are confined within some preselected compact sets all the time. This is achieved by means of barrier Lyapunov functions which have been successfully applied to various kinds of nonlinear systems such as strict-feedback and pure-feedback dynamics. Besides, our developed optimal controller requires less conditions on system dynamics than some existing approaches concerning optimal control. The effectiveness of the proposed optimal control approach is eventually validated by numerical as well as practical examples.
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http://dx.doi.org/10.1109/TNNLS.2021.3051030DOI Listing
January 2021

Nomograms for Predicting Non-remission in Patients Who Underwent Bariatric Surgery: A Multicenter Retrospective Study in China.

Obes Surg 2021 05 8;31(5):1967-1978. Epub 2021 Jan 8.

Affiliated Hospital of Southwest Jiaotong University, Chengdu, 610036, China.

Background: As a reflection of the increasing global incidence of obesity, there is a corresponding increase in the proportion of obese patients undergoing bariatric surgery. This study reviewed the factors and outcomes of patients who underwent bariatric surgical procedures and determined the relationships and developed a nomogram to calculate individualized patient risk.

Methods: The nomogram was based on a retrospective study on 259 patients who underwent bariatric surgery at the Chengdu Third People's Hospital from June 2017 to June 2019. The predictive accuracy and discriminative ability of the nomogram were determined by the ROC curve and C-index, respectively. The results were validated using bootstrap resampling and a retrospective study on 121 patients operated on from May 2015 to May 2019 at the Tenth People's Hospital of Shanghai.

Results: The predictors contained in the prediction nomogram included age, sex, surgical approach, hyperlipidemia, blood pressure (BP), hyperuricemia, body mass index (BMI), and waist circumference (WC). The 6-month model displayed good discrimination with a C-index of 0.765 (95% CI: 0.756 to 0.774) and good calibration. The 1-year model reached a C-index of 0.768 (95% CI, 0.759 to 0.777) in the training cohort.

Conclusions: The proposed nomogram resulted in more accurate non-remission prediction for patients with obesity after bariatric surgery and may provide a reference for the preoperative choice of surgical methods.
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http://dx.doi.org/10.1007/s11695-020-05206-8DOI Listing
May 2021

Association between serum beta-human chorionic gonadotropin and inflammation, oxidative stress in pregnancy-induced hypertension.

Microvasc Res 2021 05 30;135:104130. Epub 2020 Dec 30.

Department of Obstetrics and Gynecology, Liaocheng People's Hospital, No. 67 of Dongchang West Road, Liaocheng 252000, Shandong, China. Electronic address:

The aberrant increase of circulating beta-human chorionic gonadotropin (β-HCG) at early stages of pregnancy can be used to predict gestational hypertension. However, the association of β-HCG and inflammation, oxidative stress in pregnancy-caused hypertensive disorder on perinatal stage remains unclear. A case-controlled study was performed, with 133 adult pregnant women participated in their perinatal stage. Participants in this research included 45 with mild preeclampsia, 40 with severe preeclampsia and 48 without hypertension. Higher circulating β-HCG level was correlated with severer pregnancy-induced hypertension. Independent contribution of inflammatory factors including interleukin-6, tumor necrosis factor-α and interferon-γ and oxidative stress factors including thiobarbituric acid reactive substance and total antioxidant capacity to severe pregnancy-induced hypertension was significant (P < 0.001). The correlation of circulating β-HCG levels with inflammatory and oxidative stress markers in patients with pregnancy-induced hypertension in perinatal stage was statistically significant.
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http://dx.doi.org/10.1016/j.mvr.2020.104130DOI Listing
May 2021

Influence of drying methods on the structure and properties of cellulose formate and its application as a reducing agent.

Int J Biol Macromol 2021 Feb 28;170:397-405. Epub 2020 Dec 28.

CAS Key Laboratory of Biofuels, Dalian National Laboratory for Clean Energy, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China. Electronic address:

Cellulose formate (CF) with surface formyl groups can be prepared through the esterification between cellulose and formic acid (FA). The properties of CF are sensitive to temperature, which is of great importance for its end application. In this work, the effect of four drying methods on the structure and properties of the resultant CF was investigated. Results showed that the CF samples as special cellulose nanofibrils with cellulose II crystal form and fibrous structure were sensitive to drying temperature and drying time. The freeze-dried CF sample maintained its original structure, while the air-dried and oven-dried CF samples with amorphous structure showed the aggregation state. Furthermore, the CF/Ag composites were prepared using silver mirror reaction where the never dried CF was used as a reducing agent. SEM and TEM images exhibited a large number of Ag nanoparticles with the diameter of 20-50 nm on the surface of CF samples. As expected, the fabricated CF/Ag composites showed strong antibacterial activity against both Escherichia coli and Bacillus subtilis, and thus the prepared composites have great potential applications in antibacterial daily necessities and medical supplies.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.12.185DOI Listing
February 2021

Identifying 4 Novel lncRNAs as Potential Biomarkers for Acute Rejection and Graft Loss of Renal Allograft.

J Immunol Res 2020 28;2020:2415374. Epub 2020 Nov 28.

Department of Urology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.

Acute rejection (AR) after kidney transplant is one of the major obstacles to obtain ideal graft survival. Reliable molecular biomarkers for AR and renal allograft loss are lacking. This study was performed to identify novel long noncoding RNAs (lncRNAs) for diagnosing AR and predicting the risk of graft loss. The several microarray datasets with AR and nonrejection specimens of renal allograft downloaded from Gene Expression Omnibus database were analyzed to screen differentially expressed lncRNAs (DElncRNAs) and mRNAs (DEmRNAs). Univariate and multivariate Cox regression analyses were used to identify optimal prognosis-related DElncRNAs for constructing a risk score model. 39 common DElncRNAs and 185 common DEmRNAs were identified to construct a lncRNA-mRNA regulatory relationship network. DElncRNAs were revealed to regulate immune cell activation and proliferation. Then, 4 optimal DElncRNAs, ATP1A1-AS1, CTD-3080P12.3, EMX2OS, and LINC00645, were selected from 17 prognostic DElncRNAs to establish the 4-lncRNA risk score model. In the training set, the high-risk patients were more inclined to graft loss than the low-risk patients. Time-dependent receiver operating characteristics analysis revealed the model had good sensitivity and specificity in prediction of 1-, 2-, and 3-year graft survival after biopsy (AUC = 0.891, 0.836, and 0.733, respectively). The internal testing set verified the result well. Gene set enrichment analysis which expounded NOD-like receptor, the Toll-like receptor signaling pathways, and other else playing important role in immune response was enriched by the 4 lncRNAs. Allograft-infiltrating immune cells analysis elucidated the expression of 4 lncRNAs correlated with gamma delta T cells and eosinophils, etc. Our study identified 4 novel lncRNAs as potential biomarkers for AR of renal allograft and constructed a lncRNA-based model for predicting the risk of graft loss, which would provide new insights into mechanisms of AR.
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http://dx.doi.org/10.1155/2020/2415374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7739051PMC
November 2020

Aberrant Amplitude of Low-Frequency Fluctuations in Different Frequency Bands in Patients With Parkinson's Disease.

Front Aging Neurosci 2020 3;12:576682. Epub 2020 Dec 3.

Department of Radiology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, China.

Previous studies reported abnormal spontaneous neural activity in Parkinson's disease (PD) patients using resting-state functional magnetic resonance imaging (R-fMRI). However, the frequency-dependent neural activity in PD is largely unknown. Here, 35 PD patients and 35 age- and education-matched healthy controls (HCs) underwent R-fMRI scanning to investigate abnormal spontaneous neural activity of PD using the amplitude of low-frequency fluctuation (ALFF) approach within the conventional band (typical band: 0.01-0.08 Hz) and specific frequency bands (slow-5: 0.010-0.027 Hz and slow-4: 0.027-0.073 Hz). Compared with HCs, PD patients exhibited increased ALFF in the parieto-temporo-occipital regions, such as the bilateral inferior temporal gyrus/fusiform gyrus (ITG/FG) and left angular gyrus/posterior middle temporal gyrus (AG/pMTG), and displayed decreased ALFF in the left cerebellum, right precuneus, and left postcentral gyrus/supramarginal gyrus (PostC/SMG) in the typical band. PD patients showed greater increased ALFF in the left caudate/putamen, left anterior cingulate cortex/medial superior frontal gyrus (ACC/mSFG), left middle cingulate cortex (MCC), right ITG, and left hippocampus, along with greater decreased ALFF in the left pallidum in the slow-5 band, whereas greater increased ALFF in the left ITG/FG/hippocampus accompanied by greater decreased ALFF in the precentral gyrus/PostC was found in the slow-4 band (uncorrected). Additionally, the left caudate/putamen was positively correlated with levodopa equivalent daily dose (LEDD), Hoehn and Yahr (HY) stage, and disease duration. Our results suggest that PD is related to widespread abnormal brain activities and that the abnormalities of ALFF in PD are associated with specific frequency bands. Future studies should take frequency band effects into account when examining spontaneous neural activity in PD.
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http://dx.doi.org/10.3389/fnagi.2020.576682DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744880PMC
December 2020