Publications by authors named "Yanhua Xu"

123 Publications

Onset Symptom Clusters in Multiple Sclerosis: Characteristics, Comorbidities, and Risk Factors.

Front Neurol 2021 6;12:693440. Epub 2021 Jul 6.

Swiss MS Registry, Epidemiology, Biostatistics and Prevention Institute, University of Zurich, Zurich, Switzerland.

Multiple sclerosis (MS) symptoms are expected to aggregate in specific patterns across different stages of the disease. Here, we studied the clustering of onset symptoms and examined their characteristics, comorbidity patterns and associations with potential risk factors. Data stem from the Swiss Multiple Sclerosis Registry, a prospective study including 2,063 participants by November 2019. MS onset symptoms were clustered using latent class analysis (LCA). The latent classes were further examined using information on socio-demographic characteristics, MS-related features, potential risk factors, and comorbid diseases. The LCA model with six classes (frequencies ranging from 12 to 24%) was selected for further analyses. The latent classes comprised a multiple symptoms class with high probabilities across several symptoms, contrasting with two classes with solitary onset symptoms: vision problems and paresthesia. Two gait classes emerged between these extremes: the gait-balance class and the gait-paralysis class. The last class was the fatigue-weakness-class, also accompanied by depression symptoms, memory, and gastro-intestinal problems. There was a moderate variation by sex and by MS types. The multiple symptoms class yielded increased comorbidity with other autoimmune disorders. Similar to the fatigue-weakness class, the multiple symptoms class showed associations with angina, skin diseases, migraine, and lifetime prevalence of smoking. Mononucleosis was more frequently reported in the fatigue-weakness and the paresthesia class. Familial aggregation did not differ among the classes. Clustering of MS onset symptoms provides new perspectives on the heterogeneity of MS. The clusters comprise different potential risk factors and comorbidities. They point toward different risk mechanisms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2021.693440DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290323PMC
July 2021

The Impact of General Self-Efficacy on Psychological Resilience During the COVID-19 Pandemic: The Mediating Role of Posttraumatic Growth and the Moderating Role of Deliberate Rumination.

Front Psychol 2021 23;12:684354. Epub 2021 Jun 23.

Office of International Cooperation and Exchange, Dongguan University of Technology, Dongguan, China.

This study used a moderated mediation model to explore the relationship between general self-efficacy (GSE) and psychological resilience (PR) and the associated mechanisms, the mediating role of posttraumatic growth (PTG), and the moderating role of deliberate rumination (DR) during the coronavirus disease 2019 (COVID-19) pandemic. Knowledge of the relationship between these four variables examined further understanding of the PR improvement mechanism of college students and even the general public. The college students who participated in this study came from an independent college in Guangdong Province, China. A total of 918 college students completed the survey, and the final data sample size was 881. SPSS 23.0 and PROCESS (version 3.3) were used to conduct Pearson's correlation analysis and hierarchical regression linear analysis on the data. (1) The correlation analysis showed that GSE and PR were positively correlated and that PTG was positively correlated with GSE and PR. DR was positively correlated with GSE, PTG, and PR. (2) The results of mediation analysis showed that GSE had a direct predictive effect on DR, and PTG partially mediated the relationship between the two. (3) The results of moderating effect analysis showed that DR hindered the effect of GSE on PTG but enhanced the positive impact of PTG on PR. General self-efficacy can improve PR under the mediating influence of PTG. DR played a positive moderating role in the relationship between GSE and PTG, and played a negative moderating role in the relationship between PTG and PR. These results advance the understanding of the mechanism between GSE and PR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyg.2021.684354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261126PMC
June 2021

LncRNA HCP5 : A Potential Biomarker for Diagnosing Gastric Cancer.

Front Oncol 2021 18;11:684531. Epub 2021 Jun 18.

Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, China.

Background: It has been reported that long non-coding RNAs (lncRNAs) can be regarded as a biomarker and had particular clinical significance for early screening and gastric cancer (GC) diagnosis. Therefore, this study aimed to investigate whether serum HCP5 could be a new diagnostic biomarker.

Methods: Filtered out the HCP5 from the GEO database. The specificity of HCP5 was verified by real-time fluorescence quantitative PCR (qRT-PCR), and then the stability of HCP5 was verified by room temperature storage and repeated freeze-thaw experiments. Meanwhile, the accuracy of HCP5 was verified by agarose gel electrophoresis (AGE) and Sanger sequencing. Simultaneously, the expression level of serum HCP5 was detected by qRT-PCR in 98 patients with primary gastric cancer, 21 gastritis patients, 82 healthy donors, and multiple cancer types. Then, the methodology analysis was carried on. Moreover, receiver operating characteristic (ROC) was used to evaluate its diagnostic efficiency.

Results: qRT-PCR method had good repeatability and stability in detecting HCP5. The expression level of HCP5 in the serum of gastric cancer patients was remarkably higher than that of healthy controls, and it could distinguish gastritis patients from healthy donors. Besides, the expression of HCP5 was increased dramatically in MKN-45 and MGC-803. The FISH assay showed that HCP5 was mainly distributed in the cytoplasm of MKN-45 and BGC-823 cells. When HCP5 was combined with existing tumor markers, the diagnostic efficiency of HCP5 was the best, and the combined diagnosis of carcinoembryonic antigen (CEA), carbohydrate antigen199 (CA199), and HCP5 can significantly improve the diagnostic sensitivity. Besides, compared with the expression levels of thyroid cancer (THCA), colorectal cancer (CRC), and breast cancer (BRCA), serum HCP5 in gastric cancer was the most specific. Moreover, the high expression of serum HCP5 was related to differentiation, lymph node metastasis, and nerve invasion. The term of serum HCP5 after the operation was significantly lower than that of patients with primary gastric cancer.

Conclusion: Serum HCP5 can be used as a potential biomarker of non-invasive fluid biopsy, which had a unique value in the early diagnosis, development, and prognosis of gastric cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.684531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8252797PMC
June 2021

Programmed death-1 mediates venous neointimal hyperplasia in humans and rats.

Aging (Albany NY) 2021 06 24;13(12):16656-16666. Epub 2021 Jun 24.

School of Material Science and Engineering & Henan Key Laboratory of Advanced Magnesium Alloy & Key Laboratory of Materials Processing and Mold Technology, Ministry of Education, Zhengzhou University, Henan, China.

Venous neointimal hyperplasia can be a problem after vein interventions. We hypothesized that inhibiting programmed death-1 (PD-1) can decrease venous neointimal hyperplasia in a rat inferior vena cava (IVC) patch venoplasty model. The rats were divided into four groups: the control group was only decellularized without other special treatment; the PD-1 group was injected with a single dose of humanized PD-1 antibody (4 mg/kg); the PD-1 antibody coated patches group; the BMS-1 (a PD-1 small molecular inhibitor) coated patches group (PD-1 inhibitor-1). Patches were implanted to the rat IVC and harvested on day 14 and analyzed. Immunohistochemical analysis showed PD-1-positive cells in the neointima in the human samples. There was high protein expression of PD-1 in the neointima in the rat IVC venoplasty model. PD-1 antibody injection can significantly decrease neointimal thickness ( < 0.0001). PD-1 antibody or BMS-1 was successfully conjugated to the decellularized rat thoracic artery patch by hyaluronic acid with altered morphology and reduced the water contact angle (WCA). Patches coated with humanized PD-1 antibody or BMS-1 both can also decrease neointimal hyperplasia and inflammatory cells infiltration. PD-1-positive cells are present in venous neointima in both human and rat samples. Inhibition of the PD-1 pathway may be a promising therapeutic strategy to inhibit venous neointimal hyperplasia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.203185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266332PMC
June 2021

Author Correction: Biodegradable black phosphorus-based nanospheres for in vivo photothermal cancer therapy.

Nat Commun 2021 Jun 18;12(1):3923. Epub 2021 Jun 18.

Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, 999077, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-23210-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213816PMC
June 2021

High-efficiency removal of tetracycline by carbon-bridge-doped g-CN/FeO magnetic heterogeneous catalyst through photo-Fenton process.

J Hazard Mater 2021 Jun 7;418:126333. Epub 2021 Jun 7.

Jiangsu Baichuan High-Tech New Materials Co., Ltd, Nanjing, China.

Carbon-bridge-modified malonamide (MLD)/g-CN (CN) was prepared by copolymerization of MLD with urea and melamine and loaded with FeO for the high-efficiency removal of tetracycline (TC) in water under photo-Fenton. The prepared catalysts were characterized by SEM, TEM, N adsorption-desorption analysis, XPS, XRD, and FTIR, which proved that the modification method successfully introduced the C bridge into the carbon nitride molecular system and increased the structural defects of the catalyst. The Carbon-bridge-modified MLD/CN/FeO also had good visible-light response and charge-separation and transport abilities in the photoelectrochemical test. Degradation results showed that the photo-Fenton degradation of TC reached 95.8%, and the mineralization rate was 55.7% within 80 min at 80 mM HO dosage, 0.5 g/L catalyst dosage, and near-neutral pH by 0.8MLD/CN/FeO. Moreover, the oxidation products and mineralization pathways of TC were explored by LC-MS. Toxicity analysis indicated low environmental threat of the intermediates in TC mineralization. EPR analysis and HO decomposition efficiency analyses showed an improvement in the HO decomposition performance of 0.8MLD/CN/FeO. This work could provide a valuable insight for the application of heterogeneous photo-Fenton technology in wastewater treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2021.126333DOI Listing
June 2021

Preparation of Supported Perovskite Catalyst to Purify Membrane Concentrate of Coal Chemical Wastewater in UV-Catalytic Wet Hydrogen Peroxide Oxidation System.

Int J Environ Res Public Health 2021 05 4;18(9). Epub 2021 May 4.

College of Environmental Science and Engineering, Nanjing Tech University, Nanjing 211800, China.

The effective treatment of membrane concentrate is a major technical challenge faced by the new coal chemical industry. In this study, a supported perovskite catalyst LaCoO/X was prepared by a sol-impregnation two-step method. The feasibility of the supported perovskite catalyst LaCoO/X in the UV-catalytic wet hydrogen peroxide oxidation (UV-CWPO) system for the purification of concentrated liquid of coal chemical wastewater was investigated. The effects of catalyst support, calcination temperature, calcination time, and re-use time on catalytic performance were investigated by batch experiments. The catalysts were characterized by X-ray diffraction (XRD), Scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), and X-ray photoelectron spectroscopy (XPS). Experimental results showed that the supported perovskite catalyst LaCoO/CeO prepared using CeO as support, calcination temperature of 800 °C, and calcination time of 8 h had the best catalytic effect. The catalytic performance of the catalyst remained excellent after seven cycles. The best prepared catalyst was used in UV-CWPO of coal chemical wastewater membrane concentrate. The effects of HO dosage, reaction temperature, reaction pressure, and catalyst dosage on UV-CWPO were determined. Under the conditions of HO dosage of 40 mM, reaction temperature of 120 °C, reaction pressure of 0.5 MPa, catalyst dosage of 1 g/L, pH of 3, and reaction time of 60 min, the removal efficiencies of COD, TOC, and UV were 89.7%, 84.6%, and 98.1%, respectively. Under the optimal operating conditions, the oxidized effluent changed from high toxicity to non-toxicity, the BOD/COD increased from 0.02 to 0.412, and the biodegradability of the oxidized effluent was greatly improved. The catalyst has a simple synthesis procedure, excellent catalytic performance, and great potential in the practical application of coal chemical wastewater treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijerph18094906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125239PMC
May 2021

Depression and Creativity During COVID-19: Psychological Resilience as a Mediator and Deliberate Rumination as a Moderator.

Front Psychol 2021 6;12:665961. Epub 2021 May 6.

School of Geography, South China Normal University, Guangzhou, China.

The pandemic of coronavirus disease 2019 (COVID-19), which has had a significant impact on people's lives, has apparently increased the incidence of depression. Although the topic of how depression affects creativity is contested, previous research has revealed a significant relationship between the two. The purpose of this study is to further investigate the relationship and the mechanisms that operate between depression and creativity. A total of 881 students at an independent college in China completed a questionnaire consisting of the Self-Reported Depression Scale, Runco Ideational Behavior Scale, Psychological Resilience Scale, Deliberate Rumination Scale and demographic information. Among the respondents, 317 (36.0%) were male and 564 (64.0%) were female, all of whom were from the same grade. Correlation analyses were conducted, and then the researchers carried out mediation analysis and developed a moderated mediation model. The results indicated that (a) depression was positively related to creativity ( = 0.085, < 0.05); (b) psychological resilience mediated the relationship between depression and creativity; specifically, psychological resilience was negatively related to depression ( = -0.462, < 0.01), which in turn was positively related to creativity ( = 0.198, < 0.01); and (c) deliberate rumination moderated the relationship between depression and psychological resilience, showing a significant negative correlation with depression ( = 0.138, < 0.01), psychological resilience ( = 0.078, < 0.05), and creativity ( = 0.288, < 0.05); specifically, higher levels of deliberate rumination strengthened the negative correlation between psychological resilience and depression. The results suggest that depression is a positive predictor of creativity and may promote creativity to some extent. Further, individuals with greater psychological resilience are more creative than those with less psychological resilience, as it is a question of whether they can and to what extent they can effectively use depression as an emotional resource. Last, an individual's level of deliberate rumination moderates the mediating process, especially at the stage where depression is associated with psychological resilience. These findings advance understanding of the mechanisms that operate between depression and creativity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyg.2021.665961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134673PMC
May 2021

The Influence of Post-Traumatic Growth on College Students' Creativity During the COVID-19 Pandemic: The Mediating Role of General Self-Efficacy and the Moderating Role of Deliberate Rumination.

Front Psychol 2021 14;12:665973. Epub 2021 Apr 14.

School of Geography, South China Normal University, Guangzhou, China.

This study used a moderated mediation model to test the mediating effect of general self-efficacy on the relationship between post-traumatic growth (PTG) and creativity and the moderating effect of deliberate rumination in the second path of the indirect mediation path during the COVID-19 pandemic. A sample of 881 university students from Guangdong Province, China, was surveyed with the Posttraumatic Growth Inventory, the Runco Ideational Behavior Scale, the General Self-Efficacy Scale, and the Deliberate Rumination Inventory. SPSS (23 version) and PROCESS (3.3 version) were used for correlation analyses, mediation analysis, and moderated mediation analysis. (1) PTG was positively correlated with creativity, self-efficacy, and deliberate rumination. Creativity was positively correlated with self-efficacy and deliberate rumination. Deliberate rumination was positively correlated with self-efficacy. (2) Self-efficacy mediated the relationship between PTG and creativity. (3) Deliberate rumination moderated the second half of the path of "PTG → self-efficacy → creativity." PTG affected creativity directly and also indirectly through self-efficacy. In particular, deliberate rumination moderated the relationship between self-efficacy and creativity, such that the association was stronger when the incidence of deliberate rumination was low. These results provide a more comprehensive understanding of the positive link between PTG and creativity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyg.2021.665973DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079774PMC
April 2021

Hydrogel-coated needles prevent puncture site bleeding.

Acta Biomater 2021 Jul 22;128:305-313. Epub 2021 Apr 22.

The Vascular Biology and Therapeutics Program, Yale School of Medicine, New Haven, CT, USA; Departments of Surgery and of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, CT, USA. Electronic address:

Introduction: Incomplete hemostasis after vascular cannulation can cause a hematoma or pseudoaneurysm. We hypothesized that a hydrogel-coated needle would effectively and rapidly stop bleeding after vascular cannulation.

Methods: A hydrogel composed of sodium alginate, hyaluronic acid, and calcium carbonate was coated onto the surface of suture needles. Needles were observed using scanning electronic microscopy (SEM) and immunofluorescence. Cannulation was performed in both mouse and rat models; the liver, kidney, jugular vein, inferior vena cava and aorta were punctured using uncoated and hydrogel-coated needles. Needles coated with a hydrogel with and without CD34 antibody were used to puncture the rat jugular vein and aorta. Tissues were examined by histology and immunofluorescence.

Results: The hydrogel was successfully coated onto the surface of 22G and 30G needles and confirmed by SEM. Hydrogel-coated needles rapidly stopped bleeding after cannulation of the liver, kidney, jugular vein, inferior vena cava and aorta. Hydrogel-coated needles that contained CD34 antibody attracted vascular progenitor cells near the puncture site; there were fewer M1-type macrophages and more M2-type macrophages.

Conclusion: Hydrogel-coated needles can effectively and rapidly stop puncture-site bleeding. The hydrogel that contains CD34 antibody attracted vascular progenitor cells, potentially promoting healing of the site after cannulation.

Statement Of Significance: Incomplete hemostasis after vascular cannulation can cause a hematoma or pseudoaneurysm and remains a significant clinical problem. We developed a hydrogel composed of sodium alginate, hyaluronic acid, and calcium carbonate; hydrogel-coated needles effectively and rapidly stopped bleeding after vascular cannulation. Interestingly, the hydrogel can also serve as a carrier for drugs that are delivered to the puncture site during the short time of cannulation that could additionally promote puncture site healing. Hydrogel-coated needles may be a new method for rapid hemostasis with application to patients especially at risk for bleeding.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.actbio.2021.04.004DOI Listing
July 2021

The behavior of surface acidity on photo-Fenton degradation of ciprofloxacin over sludge derived carbon: Performance and mechanism.

J Colloid Interface Sci 2021 Sep 8;597:84-93. Epub 2021 Apr 8.

School of Environmental Sciences and Engineering, Nanjing Tech University, Nanjing 211816, PR China; School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, PR China. Electronic address:

Sludge derived carbon (SC) has been widely used in advanced oxidation processes as an effective and economic catalyst. In this study, we applied surface modified SC for the first time to catalyze the heterogeneous photo-Fenton process with ciprofloxacin, a highly concerned emerging contaminant, as a model substance. HSO was used to acidify the SCs under varying acid dosages, temperatures, and reaction time lengths. The surface acidity of SCs was quantitatively characterized with NH-TPD. A strong correlation between the surface acidity and the catalytic activity was clearly demonstrated. The highest catalytic activity was obtained with SC whose acidity was 0.149 mmol·g after being modified with 6 mol·L HSO at -20 ℃ for 24 h. In addition, XRD, XRF, BET, XPS, and HRTEM were also used to characterize the obtained SC. ·OH radicals were found to be the main reactive species by EPR. Ten transformation products were identified by GC-MS, based on which three possible reaction pathways were proposed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.03.156DOI Listing
September 2021

Comparison of seven in silico tools for evaluating of daphnia and fish acute toxicity: case study on Chinese Priority Controlled Chemicals and new chemicals.

BMC Bioinformatics 2021 Mar 24;22(1):151. Epub 2021 Mar 24.

Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing, 210042, China.

Background: A number of predictive models for aquatic toxicity are available, however, the accuracy and extent of easy to use of these in silico tools in risk assessment still need further studied. This study evaluated the performance of seven in silico tools to daphnia and fish: ECOSAR, T.E.S.T., Danish QSAR Database, VEGA, KATE, Read Across and Trent Analysis. 37 Priority Controlled Chemicals in China (PCCs) and 92 New Chemicals (NCs) were used as validation dataset.

Results: In the quantitative evaluation to PCCs with the criteria of 10-fold difference between experimental value and estimated value, the accuracies of VEGA is the highest among all of the models, both in prediction of daphnia and fish acute toxicity, with accuracies of 100% and 90% after considering AD, respectively. The performance of KATE, ECOSAR and T.E.S.T. is similar, with accuracies are slightly lower than VEGA. The accuracy of Danish Q.D. is the lowest among the above tools with which QSAR is the main mechanism. The performance of Read Across and Trent Analysis is lowest among all of the tested in silico tools. The predictive ability of models to NCs was lower than that of PCCs possibly because never appeared in training set of the models, and ECOSAR perform best than other in silico tools.

Conclusion: QSAR based in silico tools had the greater prediction accuracy than category approach (Read Across and Trent Analysis) in predicting the acute toxicity of daphnia and fish. Category approach (Read Across and Trent Analysis) requires expert knowledge to be utilized effectively. ECOSAR performs well in both PCCs and NCs, and the application shoud be promoted in both risk assessment and priority activities. We suggest that distribution of multiple data and water solubility should be considered when developing in silico models. Both more intelligent in silico tools and testing are necessary to identify hazards of Chemicals.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12859-020-03903-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992851PMC
March 2021

Single-Cell Transcriptome Analysis Decipher New Potential Regulation Mechanism of ACE2 and NPs Signaling Among Heart Failure Patients Infected With SARS-CoV-2.

Front Cardiovasc Med 2021 23;8:628885. Epub 2021 Feb 23.

Kelly Government Solutions, Rockville, MD, United States.

COVID-19 patients with comorbidities such as hypertension or heart failure (HF) are associated with poor clinical outcomes. The cellular distribution of Angiotensin-converting enzyme 2 (ACE2), the critical enzyme for SARS-CoV-2 infection, in the human heart is unknown. We explore the underlying mechanism that leads to increased susceptibility to SARS-CoV-2 in patients with cardiovascular diseases and patients of cardiac dysfunction have increased risk of multi-organ injury compared with patients of normal cardiac function. We analyzed single-cell RNA sequencing (scRNA-seq) data in both normal and failing hearts. The results demonstrated that ACE2 is present in cardiomyocytes (CMs) and non-CMs, while the number of ACE2-postive (ACE2+) CMs and ACE2 gene expression in these CMs are significantly increased in the failing hearts. Interestingly, both brain natriuretic peptides (BNP) and atrial natriuretic peptide (ANP) are significantly up-regulated in the ACE2+ CMs, which is consistent with other studies that ACE2, ANP, and BNP increased in HF patients. We found that genes related to virus entry, virus replication and suppression of interferon-gamma signaling are all up-regulated in failing CMs, and the increase was significantly higher in ACE2+ CMs, suggesting that these CMs may be more vulnerable to virus infection. As the level of expression of both ACE2 and BNP in CMs were up-regulated, we further performed retrospective analysis of the plasma BNP levels and clinical outcomes of 91 COVID-19 patients from a single-center. Patients with higher plasma BNP were associated with significantly higher mortality and expression levels of inflammatory and infective markers. In the failing heart, the upregulation of ACE2 and virus infection associated genes could potentially facilitate SARS-CoV-2 virus entry and replication in these vulnerable cardiomyocyte subsets. COVID-19 patients with higher plasma BNP levels had poorer clinical outcomes. These observations may allude to a potential regulatory association between ACE2 and BNP in mediating myocarditis associated with COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcvm.2021.628885DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952310PMC
February 2021

Retrospectively assessed trajectories of PTSD symptoms and their subsequent comorbidities.

J Psychiatr Res 2021 04 2;136:71-79. Epub 2021 Feb 2.

Department of Psychiatry, Psychotherapy and Psychosomatics, Psychiatric Hospital, University of Zurich, Switzerland.

Background: Dynamic trajectories of psychopathology, such as post-traumatic stress disorder (PTSD) provide a key to understanding human adjustment processes after trauma exposure. Recent studies have suggested more heterogeneous mental health outcomes than the initially identified four adjustment trajectories. To explore this heterogeneity, we investigated the after-trauma adjustment patterns of psychopathology based on retrospective lifetime data. This was first carried out on the PTSD symptoms (PTSS, including no symptoms, few symptoms, partial and full PTSD), and secondly together with their post-trauma comorbidities.

Methods: Data of trauma and the post-trauma mental disorders were collected for a large and randomly selected community sample, resulting in a N = 960 trauma-exposed subsample. Pattern recognition as carried out by latent class analysis (LCA) was implemented on this subsample. LCA was first exploited to identify the potential trajectory patterns of PTSS and next to explore the patterns of mental adjustments when additional post-trauma comorbid disorders, such as anxiety, mood and substance use disorders, were assessed.

Results: Four PTSS trajectory patterns were found, namely resilient, chronic, recovered, and delayed onset, consistent with findings from longitudinal PTSD studies. When post-trauma comorbidities were evaluated, other than the trajectory pattern of delayed onset which retained a low comorbidity profile, the other three split respectively and paired up with either low, moderate or high comorbidity profile.

Conclusions: Mental health outcomes after trauma exposure were considerably more complex than the four previously established adjustment trajectories. Here, we uncovered additional and more heterogeneous adjustment patterns comprised of PTSS trajectories and post-trauma comorbidity profiles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jpsychires.2021.01.049DOI Listing
April 2021

A novel intramural TGF β 1 hydrogel delivery method to decrease murine abdominal aortic aneurysm and rat aortic pseudoaneurysm formation and progression.

Biomed Pharmacother 2021 May 3;137:111296. Epub 2021 Feb 3.

School of Material Science and Engineering & Henan Key Laboratory of Advanced Magnesium Alloy & Key Laboratory of Materials Processing and Mould Technology (Ministry of Education), Zhengzhou University, Henan, China. Electronic address:

Objectives: Aneurysms are generally the result of dilation of all 3 layers of the vessel wall, and pseudoaneurysms are the result of localized extravasation of blood that is contained by surrounding tissue. Since there is still no recommended protocol to decrease aneurysm formation and progression, we hypothesised that intramural delivery of TGF β1 hydrogel can decrease aneurysm and pseudoaneurysm formation and progression.

Materials: Male C57BL/6 J mice (12-14 wk), SD rats (200 g) and pig abdominal aortas were used, and hydrogels were fabricated by the interaction of sodium alginate (SA), hyaluronic acid (HA) and CaCO.

Methods: A CaCl adventitial incubation model in mice and a decellularized human great saphenous vein patch angioplasty model in rats were used. TGF β1 hydrogel was intramurally delivered after CaCl incubation in mice; at day 7, the abdomen in some mice was reopened, and TGF β1 hydrogel was injected intramurally into the aorta. In rats, TGF β1 hydrogel was delivered intramurally after patch angioplasty completion. Tissues were harvested at day 14 and analysed by histology and immunohistochemistry staining. The pig aorta was also intramurally injected with hydrogel.

Results: In mice, rhodamine hydrogel was still found between the medium and adventitia at day 14. In the mouse aneurysm model, there was a thicker wall and smaller amount of elastin breaks in the TGF β1 hydrogel-delivered groups both at day 0 and day 7 after CaCl incubation, and there were larger numbers of p-smad2- and TAK1-positive cells in the TGF β1 hydrogel-injected groups. In the rat decellularized human saphenous vein patch pseudoaneurysm model, there was a higher incidence of pseudoaneurysm formation when the patch was decellularized using 3% SDS, and delivery of TGF β1 hydrogel could effectively decrease the formation of pseudoaneurysm formation and increase p-smad2 and TAK1 expression. In pig aortas, hydrogels can be delivered between the medium and adventitia easily and successfully.

Conclusions: Intramural delivery of TGF β1 hydrogel can effectively decease aneurysm and pseudoaneurysm formation and progression in both mice and rats, and pig aortas can also be successfully intramurally injected with hydrogel. This technique may be a promising drug delivery method and therapeutic choice to decrease aneurysm and pseudoaneurysm formation and progression in the clinic.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2021.111296DOI Listing
May 2021

Association of serum chemokine ligand 21 levels with asthma control in adults.

Clinics (Sao Paulo) 2021 20;76:e1713. Epub 2021 Jan 20.

Department of Allergy, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.

Objectives: The chemokine ligand (CCL) 21 regulates the maturation, migration, and function of dendritic cells, and has been implicated in the pathogenesis of asthma. This study aimed to investigate the association between serum CCL21 levels and asthma control.

Methods: The serum levels of CCL21 and other inflammatory cytokines were analyzed in patients with asthma (n=44) and healthy controls (n=35) by enzyme-linked immunosorbent assay. IgE levels and eosinophil counts were determined by turbidimetric inhibition immunoassay and fully automatic blood analysis, respectively. The Asthma Control Test (ACT) questionnaire was used, and spirometry and fractional exhaled nitric oxide (FENO) measurements were performed. A multiple unpaired Student's t-test was performed to analyze the differences in CCL21 and interleukin levels between patients with asthma and healthy controls. The correlation of CCL21 levels with disease severity was evaluated using the Pearson's rank correlation test.

Results: Serum CCL21 levels were lower in patients with asthma (254.78±95.66 pg/mL) than in healthy controls (382.95±87.77 pg/mL) (p<0.001). Patients with asthma had significantly higher levels of IL-1β (19.74±16.77 vs. 2.63±5.22 pg/mL), IL-6 (7.55±8.65 vs. 2.37±2.47 pg/mL), and tumor necrosis factor-α (12.70±12.03 vs. 4.82±3.97 pg/mL) compared with the controls. CCL21 levels were positively correlated with the ACT score (rs=0.1653, p=0.0062), forced expiratory volume in 1s (FEV1)/forced vital capacity (rs=0.3607, p<0.0001), and FEV1 (rs=0.2753, p=0.0003), and negatively correlated with FENO (rs=0.1060, p=0.0310). CCL21 levels were negatively correlated with serum IgE levels (rs=0.1114, p=0.0268) and eosinophil counts (rs=0.3476, p<0.0001).

Conclusions: Serum CCL21 levels may be a new biomarker for assessing asthma control.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.6061/clinics/2021/e1713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798131PMC
April 2021

As a biomarker for gastric cancer, circPTPN22 regulates the progression of gastric cancer through the EMT pathway.

Cancer Cell Int 2021 Jan 11;21(1):44. Epub 2021 Jan 11.

Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Xisi Road, No. 20, Nantong, 226001, Jiangsu, China.

Background: Gastric cancer (GC) is one of the most common cancers in the world. Due to the lack of specific symptoms, more than 80% of patients are diagnosed as the advanced stage with a high mortality rate, so the early diagnosis of GC is incredibly essential. Circular RNAs (CircRNAs) are a kind of endogenous non-coding RNA with stable structure, the long half-life, and tumor specificity. It can be used as a diagnostic marker for tumors.

Method: Using circRNA sequencing technology screened three pairs of GC and adjacent tissues, and circRNAs with significant expression differences were screened out. The circular structure and characteristics of circPTPN22 were determined by RT-qPCR, agarose gel electrophoresis, Sanger sequencing, RNase R, and actinomycin D assays. Cell Counting Kit-8, colony formation, Transwell, Wound healing, tumor formation in mice and western blotting assays were used to detect the effects of circPTPN22 on the proliferation, invasion, migration, tumor growth of GC cells in vitro and protein expression.

Result: CircPTPN22 is up-regulated and positively correlated with metastasis in GC tissues, cells, and plasma. RT-qPCR results showed that circPTPN22 had good diagnostic efficacy and could be used to predict the prognosis of GC patients. In vitro and vivo experiments showed that the downregulation of circPTPN22 could inhibit cell proliferation, migration, and invasion through the epithelial-mesenchymal transformation (EMT) pathway. CircPTPN22 may regulate GC progression through the competitive binding of miRNAs.

Conclusion: CircPTPN22 can be used as a potential diagnostic and prognostic marker for GC and can inhibit cell proliferation and metastasis through the competitive binding of miRNA to inhibit the EMT pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12935-020-01701-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802183PMC
January 2021

Photocatalytic degradation of organic dye and tetracycline by ternary AgO/AgBr-CeO photocatalyst under visible-light irradiation.

Sci Rep 2021 01 8;11(1):85. Epub 2021 Jan 8.

Nanjing Tech University, Nanjing, 211800, People's Republic of China.

In this work, CeO nanosheets decorated with AgO and AgBr are successfully fabricated via a simple sediment-precipitation method. The as-prepared ternary AgO/AgBr-CeO composite with double Z-scheme construction was analyzed by various analytical techniques. Ag nanoparticles (NPs) used as the electron medium could reduce the recombination of photoelectrons and holes, thus leading to the improvement of photocatalytic performance of these catalysts. Due to the unique structure and composite advantages, the optimal AgO/AgBr-CeO photocatalysts exhibit the superior tetracycline (TC) degradation efficiency of 93.23% and favorable stability with near-initial capacity under visible light irradiation. This ternary Z-scheme structure materials will be the well-promising photocatalysts or the purification of antibiotic wastewater.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-76997-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794347PMC
January 2021

MiR-519d and miR-328-3p Combinatorially Suppress Breast Cancer Progression.

Onco Targets Ther 2020 18;13:12987-12997. Epub 2020 Dec 18.

Department of Oncology, Guangrao County People's Hospital, Dongying City, Shandong Province 257300, People's Republic of China.

Background: MiR-519d and miR-328-3p had tumor-regulatory properties in different cancers, but their combinatorial effects and potential common target in breast cancer had not been fully reported. This research targeted to study the underlying mechanism of how miR-519d and miR-328-3p cooperatively suppressed breast cancer.

Methods: MiR-519d and miR-328-3p expressions in breast cancer tissues and cells were assessed and Ki-67 expression was also checked. DLR assay was executed to verify whether Ki-67 was a common target of miR-519d and miR-328-3p. Western blot, flow cytometry, colony formation, wound healing and transwell assays were applied to examine the inhibitory roles of these two miRNAs on the malignant behaviors of breast cancer cells and the potential molecular mechanism.

Results: Impeded miR-519d and miR-328-3p expressions and enhanced Ki-67 expression were detected in breast cancer tissues and cells. Ki-67 was confirmed as a target of these two miRNAs. MiR-519d and miR-328-3p hampered cell proliferation and blocked cell cycle via binding to Ki-67 and they also suppressed migration and invasion. The combinatorial effects of two miRNAs were much stronger than a single miRNA.

Conclusion: Our findings proved that miR-519d and miR-328-3p played combinatorial anti-cancer roles in breast cancer by directly targeting a common target Ki-67. Our study suggested that these two miRNAs might own the potential to become novel therapeutic biomarkers involved in the diagnosis and therapy of breast cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/OTT.S281962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755341PMC
December 2020

Exosomal circRNAs: Sorting Mechanisms, Roles and Clinical Applications in Tumors.

Front Cell Dev Biol 2020 25;8:581558. Epub 2020 Nov 25.

Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, China.

Exosomes are a group of nano-sized membrane vesicles and are important mediators of intercellular communication, particularly in tumor microenvironment. Recently, researchers have found that circular RNAs (circRNAs), with the great research significance, are enriched and stable in exosomes. In this review, we summarize the research significance of exosomal circRNAs, sorting mechanisms and their functioning mechanisms in tumor progression. Their clinical applications as clinical tumor biomarkers and as therapeutic targets in inhibiting tumor metastasis, anti-cancer immunity response and drug resistance have been widely discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2020.581558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723975PMC
November 2020

Synthesis and evaluation of cationic flocculant P(DAC-PAPTAC-AM) for flocculation of coal chemical wastewater.

J Environ Sci (China) 2021 Jan 8;99:239-248. Epub 2020 Jul 8.

School of Environmental Sciences and Engineering, Nanjing Tech University, Nanjing 211816, China.

In this study, a high-efficiency cationic flocculant, P(DAC-MAPTAC-AM), was successfully prepared using UV-induced polymerization technology. The monomer Acrylamide (AM): Acryloxyethyl Trimethyl ammonium chloride (DAC): methacrylamido propyl trimethyl ammonium chloride (MAPTAC) ratio, monomer concentration, photoinitiator concentration, urea content, and cationic monomer DAC:MAPTAC ratio, light time, and power of high-pressure mercury lamp were studied. The characteristic groups, characteristic diffraction peaks, and characteristic proton peaks of P(DAC-MAPTAC-AM) were confirmed by fourier transform infrared spectroscopy (FTIR), X-Ray diffraction (XRD), H nuclear magnetic resonance spectrometer (H NMR), and scanning electron microscopy (SEM). The effects of dosage, pH value, and velocity gradient (G) value on the removal efficiencies of turbidity, COD, ammonia nitrogen, and total phenol by poly aluminum ferric chloride (PAFC), P(DAC-MAPTAC-AM), and PAFC/P(DAC-MAPTAC-AM) in the flocculation treatment of coal chemical wastewater were investigated. Results showed that the optimal conditions for the flocculation of coal chemical wastewater using P(DAC-MAPTAC-AM) alone are as follows: dosage of 8-12 mg/L, G value of 100-250 s  , and pH value of 4-8. The optimal dosage of PAFC is 90-150 mg/L with a pH of 2-12. The optimal dosage for PAFC/P(DAC-MAPTAC-AM) is as follows: PAFC dosage of 90-150 mg/L, P(DAC-MAPTAC-AM) dosage of 8-12 mg/L, and pH range of 2-6. When P(DAC-MAPTAC-AM) was used alone, the optimal removal efficiencies of turbidity, COD, ammonia nitrogen, and total phenol were 81.0%, 35.0%, 75.0%, and 80.3%, respectively. PAFC has good tolerance to wastewater pH and good pH buffering. Thus, the flocculation treatment of coal chemical wastewater using the PAFC/P(DAC-MAPTAC-AM) compound also exhibits excellent resistance and buffering capacity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jes.2020.07.001DOI Listing
January 2021

Comprehensive Assessment of Plasma Circ_0004771 as a Novel Diagnostic and Dynamic Monitoring Biomarker in Gastric Cancer.

Onco Targets Ther 2020 8;13:10063-10074. Epub 2020 Oct 8.

Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong 226001, People's Republic of China.

Purpose: Due to the lack of specific and sensitive detection indicators, most patients with GC are already in the advanced stage at the time of diagnosis. Therefore, it is urgent to search for effective diagnostic biomarkers that can be applied in clinic.

Materials And Methods: We screened out circ_0004771 through circRNA sequencing. Exonuclease digestion assay, agarose gel electrophoresis (AGE) and Sanger sequencing verified the potential of circ_0004771 being a biomarker. Additionally, we established quantitative real-time fluorescent polymerase chain reaction (qRT-PCR) to detect the expression level of circ_0004771 and evaluated the methodology. What's more, we collected plasma samples from 120 GC patients, 40 superficial gastritis patients, 20 postoperative GC patients, 20 postoperative recurrence patients and 120 healthy donors. We constructed the receiver operating characteristic curve (ROC) to appraise its diagnostic efficacy.

Results: The expression level of circ_0004771 is up-regulated in GC tissues, which is consistent with circRNA sequencing result (P=0.0001). Circ_0004771 can serve as a promising biomarker because of its stable structure and longer half-life. Plasma circ_0004771 expression is markedly richer in GC patients than that in normal people (P<0.0001), and the area under the ROC (AUC) is 0.831 (95% CI: 0.779-0.883). The diagnostic efficacy of circ_0004771 is higher than that of CEA (AUC=0.747, 95% CI: 0.686-0.808) and CA199 (AUC=0.508, 95% CI: 0.433-0.583). Higher diagnostic efficacy can be achieved by combination diagnosis for distinguishing GC patients from normal people (AUC=0.864). Besides, the expression level of circ_0004771 can distinguish GC patients from gastritis patients (AUC=0.845, 95% CI: 0.772-0.917). The plasma circ_0004771 expression in GC patients decreased to normal after surgery (P<0.0001). In addition, plasma circ_0004771 expression increased again in patients with postoperative recurrence.

Conclusion: Plasma circ_0004771 is differentially expressed in GC patients, postoperative GC patients and patients with recurrence, suggesting that plasma circ_0004771 can be used as a novel diagnostic and dynamic monitoring biomarker in GC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/OTT.S263536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549879PMC
October 2020

Non-thermal plasma combined with zeolites to remove ammonia nitrogen from wastewater.

J Hazard Mater 2021 01 11;401:123627. Epub 2020 Aug 11.

School of Environmental Science and Engineering, Nanjing Tech University, Nanjing 211816, China.

In this work, non-thermal plasma combined with zeolites was used to remove inorganic pollutant ammonia nitrogen from wastewater. Ammonia nitrogen elimination performances at various operating parameters were investigated. Roles of active species in the removal of ammonia nitrogen were also discussed. The experimental results showed that 69.97% ammonia nitrogen can be removed from the plasma/zeolites synergistic system after 30 min treatment. The removal efficiency was 16.23% and 61.55% higher than that in sole zeolites adsorption system and that in sole discharge plasma system, respectively. Higher applied voltage, lower initial ammonia nitrogen concentration and weak acidic conditions were favorable for ammonia nitrogen removal. After the addition of zeolites, part of O and HO generated in the plasma/zeolites system were decomposed into other oxygen species (•OH and O), which improved the oxidation degree of ammonia nitrogen. In addition, the reaction mechanism of ammonia nitrogen in water by plasma/zeolites process was discussed. After repeated use three times, the effect of the zeolites in the plasma/zeolites system remained stable. Characterization of the zeolites after reaction was analyzed through BET, SEM, XRD and FT-IR. The experiments have confirmed the applicability of the plasma/zeolites system for the further treatment of low-concentration ammonia nitrogen wastewater.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2020.123627DOI Listing
January 2021

Identification of Fibroblast Activation Protein as an Osteogenic Suppressor and Anti-osteoporosis Drug Target.

Cell Rep 2020 10;33(2):108252

Institute for Regenerative Medicine, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China. Electronic address:

Osteogenic suppressors such as Sclerostin not only regulate skeletal development and regeneration but also serve as anti-osteoporosis drug targets. However, very few druggable suppressors have been identified due to limited understanding of the molecular mechanisms governing osteogenesis. Here, we show that fibroblast activation protein (Fap), a serine protease inhibited by the bone growth factor Osteolectin, is an osteogenic suppressor. Genetic deletion of Fap significantly ameliorates limb trabecular bone loss during aging. Pharmacological inhibition of Fap significantly promotes bone formation and inhibits bone resorption in wild-type mice by differentially regulating canonical Wnt and nuclear factor κB (NF-κB) pathways. Pharmacological inhibition of Fap promotes osteoblast differentiation, inhibits osteoclast differentiation, and significantly attenuates osteoporosis in ovariectomized mice. Epistasis analyses in zebrafish show that Osteolectin functions as an endogenous inhibitor of Fap to promote vertebrae mineralization. Taken together, we identify Fap as an important osteogenic suppressor and a potential drug target to treat osteoporosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.celrep.2020.108252DOI Listing
October 2020

Effective and simultaneous removal of organic/inorganic arsenic using polymer-based hydrated iron oxide adsorbent: Capacity evaluation and mechanism.

Sci Total Environ 2020 Nov 29;742:140508. Epub 2020 Jun 29.

School of Environmental Sciences and Engineering, Nanjing Tech University, Nanjing 211800, China. Electronic address:

In this study, resin-based hydrated iron oxide (HFOR) composites were prepared and used as a functional adsorbent for the simultaneous removal of p-Arsanilic acid (p-ASA) and arsenate (As (V)). The effects of solution pH and coexisting substances on the adsorption of different arsenic species were also investigated. Results showed that the coexisting substances slightly affected the adsorption process of two arsenic species. Analysis of the adsorption behavior, isotherm equilibrium, and adsorption kinetics, as well as that results of the X-ray photoelectron spectroscopy, zeta potential, and other analytical methods revealed that the satisfactory adsorption performance of HFOR can be attributed to the electrostatic interactions induced by the positively charged groups and the coordination of the hydrated iron oxide nanoparticles, which exhibited excellent specific adsorption for both arsenic species. Moreover, HFOR showed high acid and alkali resistance and reusability, as well as a constant co-removal performance for different arsenic species in five consecutive operating cycles (55 mg As/g of As(V) and 18 mg/g of p-ASA). Results of continuous running fixed-bed column experiments confirmed that HFOR enabled excellent simultaneous adsorption for p-ASA and As(V).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2020.140508DOI Listing
November 2020

Single-cell Transcriptome Analysis Indicates New Potential Regulation Mechanism of ACE2 and NPs signaling among heart failure patients infected with SARS-CoV-2.

medRxiv 2020 May 15. Epub 2020 May 15.

Integrative Bioinformatics, ESCBL, NIEHS, National Institutes of Health, Research Triangle Park, NC, 27709, USA.

Background: COVID-19 patients with comorbidities such as hypertension or heart failure (HF) are associated with poor clinical outcomes. Angiotensin-converting enzyme 2 (ACE2), the critical enzyme for SARS-CoV-2 infection, is broadly expressed in many organs including heart. However, the cellular distribution of ACE2 in the human heart, particularly the failing heart is unknown.

Methods: We analyzed single-cell RNA sequencing (scRNA-seq) data in both normal and failing hearts, and characterized the ACE2 gene expression profile in various cell subsets, especially in cardiomyocyte subsets, as well as its interaction with gene networks relating to various defense and immune responses at the single cell level.

Results: The results demonstrated that ACE2 is present in cardiomyocytes (CMs), endothelial cells, fibroblasts and smooth muscle cells in the heart, while the number of ACE2-postive (ACE2+) CMs and ACE2 gene expression in these CMs are significantly increased in the failing hearts. Interestingly, both brain natriuretic peptides (BNP) and atrial natriuretic peptide (ANP) are significantly up-regulated in the ACE2+ CMs. Further analysis shows that ANP, BNP and ACE2 may form a negative feedback loop with a group of genes associated with the development of heart failure. To our surprise, we found that genes related to virus entry, virus replication and suppression of interferon-gamma(IFN-γ)signaling are all up-regulated in CMs in failing hearts, and the increases were significantly higher in ACE2+ CMs as compared with ACE2 negative (ACE2-) CMs, suggesting that these ACE2+ CMs may be more vulnerable to virus infection. Since ACE2 expression is correlated with BNP expression, we further performed retrospective analysis of the plasma BNP levels and clinic outcome of 91 COVID-19 patients from a single-center. Patients with higher plasma BNP were associated with significantly higher mortality rate and expression levels of inflammatory and infective markers such as procalcitonin and C-reactive protein.

Conclusion: In the failing heart, the upregulation of ACE2 and virus infection associated genes, as well as the increased expression of ANP and BNP could facilitate SARS-CoV-2 virus entry and replication in these vulnerable cardiomyocyte subsets. These findings may advance our understanding of the underlying molecular mechanisms of myocarditis associated with COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1101/2020.04.30.20081257DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241106PMC
May 2020

Static magnetic fields accelerate osteogenesis by regulating FLRT/BMP pathway.

Biochem Biophys Res Commun 2020 06 27;527(1):83-89. Epub 2020 Apr 27.

Department of Orthodontics, Affiliated Stomatology Hospital of Kunming Medical University, Kunming, Yunnan, 650000, China. Electronic address:

Objectives: Static magnetic fields (SMF) have been proved to enhance osteogenic differentiation in mesenchymal stem cells (MSCs). However, the effect of SMF on mandibular condylar chondrocytes (MCCs) are less investigated, which contributes to the vertical formation of mandible. The purpose of the present study was to identify whether SMF accelerate the osteogenesis on mature condylar cartilage and explore the potential regulatory mechanism.

Methods: In this study, we presented a 280 mT SMF stimulation set-up to investigate the genomic effects of SMF exposure on MCCs differentiation and osteoblast-related factor secretion in vitro. Induced by Oricell™ for osteogenesis, MCCs from primary SD Rat were stimulated with or without SMF for cell culture. Cell proliferation was determined by CCK-8. The enhanced osteogenetic capacity of the SMF stimulated MCCs was identified by Alizarin red staining (ARS). Additionally, the effects of SMF on the expression of transmembrane protein marker (FLRT3), terminal differentiation markers (BMP2), and transcription factors (Smad1/5/8) were quantified by Western blot and immunofluorescence analysis.

Results: Compared with the control group, SMF decreased the proliferation of MCCs (p < 0.05) after 14 days osteogenesis-specific induction. The mineral synthesis of MCCs was upregulated by SMF (p < 0.0001). The expression of BMP2, Smad1/5/8 showed decrease trends while the protein level of FLRT3 acted in contrary manner (p < 0.05).

Conclusions: Our findings emphasized the ability of osteogenesis positively respond to SMF stimulation by exhibiting enhanced differentiation via FLRT/BMP signaling.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrc.2020.04.090DOI Listing
June 2020

miR-199 family contributes to regulation of sonic hedgehog expression during craniofacial development.

Dev Dyn 2020 09 4;249(9):1062-1076. Epub 2020 Aug 4.

Department of Orthopaedic Surgery, San Francisco General Hospital, University of California, San Francisco, San Francisco, California, USA.

Background: The frontonasal ectodermal zone (FEZ) is a signaling center that regulates patterned development of the upper jaw, and Sonic hedgehog (SHH) mediates FEZ activity. Induction of SHH expression in the FEZ results from SHH-dependent signals from the brain and neural crest cells. Given the role of miRNAs in modulating gene expression, we investigated the extent to which miRNAs regulate SHH expression and FEZ signaling.

Results: In the FEZ, the miR-199 family appears to be regulated by SHH-dependent signals from the brain; expression of this family increased from HH18 to HH22, and upon activation of SHH signaling in the brain. However, the miR-199 family is more broadly expressed in the mesenchyme of the frontonasal process and adjacent neuroepithelium. Downregulating the miR-199 genes expanded SHH expression in the FEZ, resulting in wider faces, while upregulating miR-199 genes resulted in decreased SHH expression and narrow faces. Hypoxia inducible factor 1 alpha (HIF1A) and mitogen-activated protein kinase kinase kinase 4 (MAP3K4) appear to be potential targets of miR-199b. Reduction of MAP3K4 altered beak development but increased apoptosis, while reducing HIF1A reduced expression of SHH in the FEZ and produced malformations independent of apoptosis.

Conclusions: Our results demonstrate that this miRNA family appears to participate in regulating SHH expression in the FEZ; however, specific molecular mechanisms remain unknown.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/dvdy.191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7484444PMC
September 2020

Enhanced removal of tris(2-chloroethyl) phosphate using a resin-based nanocomposite hydrated iron oxide through a Fenton-like process: Capacity evaluation and pathways.

Water Res 2020 May 28;175:115655. Epub 2020 Feb 28.

Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, State Ministry of Education, Chongqing University, Chongqing, 400045, China.

The effective removal of organophosphorus compounds (OPs) effectively from water environment remains an important but challenging task. In this study, a resin-based nanocomposite of hydrated iron oxide (HD1) was used as Fenton-like catalyst for effectively catalyzing the decomposition of hydrogen peroxide to degrade tris(2-chloroethyl) phosphate (TCEP). The results showed that HD1 was successfully prepared, which had great versatility, catalytic performance and adsorption capacity. Besides, HD1/HO was capable of degrading TCEP completely with less than 0.2 mg/L of inorganic phosphorus (IP) in the effluent at the initial TCEP of 38 mg/L, pH = 4, HO dosage of 20 mM, and the K could result in about 1.0530 min under identical conditions. More attractively, inorganic ions (i.e., Cl-, CO-, SO-, NO-, HCO-, Ca, and Mg) exhibited moderate effect on TCEP degradation. The negative effect of natural organic matters (NOM) (i.e., HA) on the degradation of TCEP was responsible for competition for the active oxygen species. Combined with electron paramagnetic resonance (EPR) spectra, X-ray photoelectron spectroscopy (XPS) and other analytical methods and radical quenching experiments, the possible removal process of TCEP was discussed, including two processes of oxidative degradation and immobilization of IP. Besides, hydroxyl radicals (•OH) was the key active species that contributed to TCEP degradation through hydroxylation-oxidation and C-O bond cracking, and specificity adsorption of HFO on IP was revealed. Furthermore, the results showed that HD1 had desirable acid and alkali resistance. In the continuous running fixed bed column experiment, HD1 showed a satisfactory performance in cycle operations. This work proposed a new enhanced process for removing TCEP in water environment by HD1/HO, and the multi-functional material, HD1 was promising in treatment of water containing organic phosphorus pollutants. This will be believed that this study will provide new ideas and new materials for the treatment of organic phosphorus-based organic pollutants, and lay the foundation for further deepening and expanding the application of adsorption resins in the field of water pollution control.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.watres.2020.115655DOI Listing
May 2020

Base-triggered self-amplifying degradable polyurethanes with the ability to translate local stimulation to continuous long-range degradation.

Chem Sci 2020 Mar 3;11(12):3326-3331. Epub 2020 Mar 3.

Department of Chemistry, University of Illinois at Urbana-Champaign Urbana Illinois 61801 USA

A new type of base-triggered self-amplifying degradable polyurethane is reported that degrades under mild conditions, with the release of increasing amounts of amine product leading to self-amplified degradation. The polymer incorporates a base-sensitive Fmoc-derivative into every repeating unit to enable highly sensitive amine amplified degradation. A sigmoidal degradation curve for the linear polymer was observed consistent with a self-amplifying degradation mechanism. An analogous cross-linked polyurethane gel was prepared and also found to undergo amplified breakdown. In this case, a trace amount of localized base initiates the degradation, which in turn propagates through the material in an amplified manner. The results demonstrate the potential utility of these new generation polyurethanes in enhanced disposability and as stimuli responsive materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9sc06582bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152679PMC
March 2020
-->