Publications by authors named "Yanhong Wang"

334 Publications

Controlled synthesis of [email protected]/NF nanoarrays as binder-free electrodes for water splitting.

Dalton Trans 2021 Jul 24. Epub 2021 Jul 24.

School of Science, North University of China, Taiyuan 030051, People's Republic of China.

The design and development of robust and environmentally friendly electrocatalytic materials are of great significance to the hydrogen production industry for the electrolysis of water. A series of [email protected]/NF materials was firstly successfully synthesized by a hydrothermal method, high temperature calcination and an electrochemical deposition approach when sodium hypophosphite was used as the source of P and Ni(NO3)2·6H2O as the source of nickel and introduced cobalt at the same time. The structure, composition, morphology and electrochemical performance of the [email protected]/NF electrocatalytic material were determined by X-ray diffraction, X-ray photoelectron spectroscopy, scanning electron microscopy and electrochemical performance testing. It is worth noting that the [email protected]/NF material presents excellent hydrogen evolution reaction performance in 1 M KOH alkaline solution. It only needs an overpotential of 181 mV to drive a current density of 100 mA cm-2, which is one of the best catalytic activities reported so far. The experimental results and theoretical calculations demonstrate that the electrocatalytic activity of the [email protected]/NF material is attributed to the faster electron transfer rate, exposure of more active sites, optimal water adsorption energy and better electrical conductivity.
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http://dx.doi.org/10.1039/d1dt01883cDOI Listing
July 2021

A case of chlamydia psittaci caused severe pneumonia and meningitis diagnosed by metagenome next-generation sequencing and clinical analysis: a case report and literature review.

BMC Infect Dis 2021 Jun 30;21(1):621. Epub 2021 Jun 30.

Medical Intensive Care Unit, Department of Respiratory and Critical Care Medicine, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, China.

Background: Psittacosis, which is also known as parrot fever, is Chlamydia psittaci (C. psittaci) caused infectious disease. The clinical manifestations vary from asymptomatic infection to severe atypical pneumonia or even fatal meningitis. Early recognition of psittacosis is difficult because of its nonspecific clinical manifestations. Culture and gene probe techniques for C. psittaci are not available for routine clinical use, which makes the diagnosis difficult too. Although psittacosis has increasingly been recognized and reported in recent years, cure of severe pneumonia complicated with meningitis, with etiologic diagnosis aided by the use of metagenomic next-generation sequencing (mNGS), is still uncommon. So, it is necessary to report and review such potentially fatal case.

Case Presentation: This report describes a 54-year-old woman with C. psittaci caused severe atypical pneumonia and meningitis. She presented with symptoms of fever, dry cough and dyspnea, accompanied by prominent headache. Her condition deteriorated rapidly to respiratory failure and lethargy under the treatment of empirical antibacterial agents, and was treated with invasive mechanical ventilation soon. She denied contact with birds, poultry or horses, but unbiased mNGS of both the bronchoalveolar lavage fluid (BALF) and the cerebrospinal fluid (CSF) identified sequence reads corresponding to C. psittaci infection, and there was no sequence read corresponding to other probable pathogens. Combined use of targeted antimicrobial agents of tetracyclines, macrolides and fluoroquinolones was carried out, and the patient's condition improved and she was discharged home 28 days later. Her status returned close to premorbid condition on day 60 of follow-up.

Conclusions: When clinicians come across a patient with atypical pneumonia accompanied by symptoms of meningitis, psittacosis should be taken into consideration. mNGS is a promising detection method in such condition and is recommended.
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http://dx.doi.org/10.1186/s12879-021-06205-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243071PMC
June 2021

Breast Cancer Resistance to Cyclin-Dependent Kinases 4/6 Inhibitors: Intricacy of the Molecular Mechanisms.

Front Oncol 2021 26;11:651541. Epub 2021 May 26.

Department of Breast Surgery, First Hospital of Shanxi Medical University, Taiyuan, China.

Breast cancer is a common malignant tumor in women, with a highest incidence and mortality among all of the female malignant tumors. Notably, targeted therapy has achieved impressive success in the treatment of breast cancer. As one class of the anti-tumor targeted therapeutics, Cyclin-Dependent Kinases 4/6CDK4/6inhibitors have shown good clinical activity in treating breast cancer. Nevertheless, despite the promising clinical outcomes, intrinsic or acquired resistance to CDK4/6 inhibitors has limited the benefits of this novel target therapy. In the present review, we provide an overview of the currently known molecular mechanisms of resistance to CDK4/6 inhibitors, and discuss the potential strategies to overcoming drug resistance improving the outcomes for breast cancer patients treated with CDK4/6 inhibitors.
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http://dx.doi.org/10.3389/fonc.2021.651541DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187902PMC
May 2021

SNP discovery of gene and their associations with growth traits in goats.

Anim Biotechnol 2021 Jun 9:1-7. Epub 2021 Jun 9.

Institute of Cellular and Molecular Biology, Jiangsu Normal University, Xuzhou, China.

AMPK plays an important role in regulating the metabolism of carbohydrate, lipid and protein in an organism, and is considered to be a key regulator of cellular energy homeostasis. In recent years, attention has been drawn to AMPK subunit polymorphisms and their association with economical traits of domestic animals and fowls. PRKAB1 encodes the β1 regulatory subunit of AMPK, and it has been reported that PRKAB1 may be applied in breeding programs of meat-type chicken. To date, the polymorphism of goat PRKAB1 gene and its associations remain unknown. In this paper, the polymorphism of gene was detected in 316 goats of three breeds. A total of four novel single nucleotide polymorphisms (SNPs) of PRKAB1 gene were revealed by sequence analysis. Among them, three were in the coding region (285 C > A, 297 C > A, 309 C > T), and they were all synonymous. One was in the intron (229 A > G). The associations between polymorphic loci and the growth traits of Xuhuai and Haimen goats were analyzed, and significant associations were found in body length index and trunk index ( < 0.05) for Xuhuai breed, while no significant associations in Haimen breed. Our results provide useful information for the improvement and breeding of Chinese native goats.
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http://dx.doi.org/10.1080/10495398.2021.1920426DOI Listing
June 2021

Predicting Risk of Pulmonary Arterial Hypertension in Systemic Lupus Erythematosus: CSTAR Cohort Study.

Arthritis Rheumatol 2021 Jun 9. Epub 2021 Jun 9.

Department of Rheumatology and Clinical Immunology, Chinese Academy of Medical Sciences & Peking Union Medical College, National Clinical Research Center for Dermatologic and Immunologic Diseases (NCRC-DID), Ministry of Science & Technology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital (PUMCH), Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, China.

Objectives: Pulmonary arterial hypertension is a life-threatening complication of systemic lupus erythematosus. However, there is no algorithm to identify those at high risk. We aimed to develop a prediction model for pulmonary arterial hypertension in lupus patients that provides individualized risk estimates.

Methods: A multicenter, longitudinal cohort study was undertaken from January 2003 to January 2020. The study collected data on 3,624 consecutively evaluated patients diagnosed with lupus. The diagnosis of pulmonary arterial hypertension was confirmed by right heart catheterization. Cox proportional hazards regression and least absolute shrinkage and selection operator were used to fit the model. Model discrimination, calibration, and decision curve analysis were assessed for validation.

Results: Ninety-two lupus patients developed pulmonary arterial hypertension (2.54%) at a median follow-up of 4.84 years (interquartile range, 2.42-8.84). The final prediction model included five clinical variables (acute/subacute cutaneous lupus, arthritis, renal disorder, thrombocytopenia, and interstitial lung disease) and three autoantibodies (anti-RNP, anti-Ro/SSA and anti-La/SSB). A 10-year pulmonary arterial hypertension probability-predictive nomogram was established. The model was internally validated by C statistic (0.78), the Brier score (0.03), and a satisfactory calibration curve. According to the net benefit and predicted probability thresholds, we recommend annual screening in high-risk (> 4.62 %) lupus patients.

Conclusion: We developed a risk stratification model using routine clinical assessments. This new tool may effectively predict the future risk of pulmonary arterial hypertension in patients with systemic lupus erythematosus.
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http://dx.doi.org/10.1002/art.41740DOI Listing
June 2021

Doxorubicin-Induced Cognitive Impairment: The Mechanistic Insights.

Front Oncol 2021 13;11:673340. Epub 2021 May 13.

Department of Breast Surgery, First Hospital of Shanxi Medical University, Taiyuan, China.

Chemotherapy can significantly prolong the survival of patients with breast cancer; Nevertheless, the majority of patients receiving chemotherapy such as doxorubicin may have cognitive deficits that manifest as impairments in learning, reasoning, attention, and memory. The phenomenon of chemotherapy-induced cognitive decline is termed as chemotherapy-related cognitive impairment (CRCI) or chemo-brain. Doxorubicin (DOX), a commonly used drug in adjuvant chemotherapy for patients with breast cancer, has been reported to induce chemo-brain through a variety of mechanisms including DNA damage, oxidative stress, inflammation, dysregulation of apoptosis and autophagy, changes in neurotransmitter levels, mitochondrial dysfunction, glial cell interactions, neurogenesis inhibition, and epigenetic factors. These mechanisms do not operate independently but are inter-related, coordinately contributing to the development of chemo-brain. Here we review the relationships of these mechanisms and pathways in attempt to provide mechanistic insights into the doxorubicin-induced cognitive impairment.
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http://dx.doi.org/10.3389/fonc.2021.673340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158153PMC
May 2021

The p70S6K/PI3K/MAPK feedback loop releases the inhibition effect of high-dose rapamycin on rat mesangial cell proliferation.

Int J Immunopathol Pharmacol 2021 Jan-Dec;35:20587384211000544

Department of Nephrology, The Affiliated People's Hospital of Shanxi Medical University, Shanxi Provincial People's Hospital, Shanxi Kidney Disease Institute, Taiyuan, China.

Glomerular mesangial cell (MC) proliferation is one of the causative factors of glomerular diseases and one of their prominent pathological features. Rapamycin can inhibit MC proliferation and slow the progression to chronic renal fibrosis. The present study was designed to observe the role of rapamycin in MC proliferation and to explore the mechanism by which rapamycin acts on Akt and MAPK/ERK1/2 pathways in mesangial cells. MTT assay and flow cytometry were used to evaluate the proliferation and the cell cycle phase of glomerular mesangial cells respectively. The mRNA expression level of p70S6K was detected by RT-qPCR. Western blotting was performed to determine p70S6K, PI3K/Akt, and PI3K/MAPK protein expression. We found that rapamycin could reduce mesangial cell proliferation and arrest the cell cycle in the G1 phase, however the inhibition effect of 1000 nmol/L rapamycin was not higher than that in the 100 nmol/L group. The results of western blotting showed that 1000 nmol/L rapamycin more significantly inhibited the phosphorylation of p70S6K than 100 nmol/L, suggesting there should be another signaling pathway that activates the proliferation of MCs. Moreover, our results revealed that 1000 nmol/L rapamycin led to Raf1-MEK1/2-ERK pathway activation through a p70S6K-PI3K-mediated feedback loop in MCs. This study demonstrated that high-dose rapamycin leads to ERK1/2 activation through a p70S6K/PI3K/MAPK feedback loop in rat MCs, thus reducing the inhibitory effect of rapamycin on MC proliferation.
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http://dx.doi.org/10.1177/20587384211000544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161859PMC
May 2021

Epigenetic regulation of intestinal peptide transporter PEPT1 as a potential strategy for colorectal cancer sensitization.

Cell Death Dis 2021 May 24;12(6):532. Epub 2021 May 24.

National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 518116, Shenzhen, China.

Human intestinal peptide transporter PEPT1 is commonly repressed in human colorectal cancer (CRC), yet its relationship with sensitivity to the common CRC treatment ubenimex has not previously been elucidated. In this study, we confirmed PEPT1 suppression in CRC using real-time quantitative polymerase chain reaction and western blotting and then investigated the underlying epigenetic pathways involved using bisulfite sequencing, chromatin immunoprecipitation, siRNA knockdown, and reporter gene assays. We found that PEPT1 transcriptional repression was due to both DNMT1-mediated DNA methylation of the proximal promoter region and HDAC1-mediated histone deacetylation, which blocked P300-mediated H3K18/27Ac at the PEPT1 distal promoter. Finally, the effects of the epigenetic activation of PEPT1 on the CRC response to ubenimex were evaluated using sequential combination therapy of decitabine and ubenimex both in vitro and in xenografts. In conclusion, epigenetic silencing of PEPT1 due to increased DNMT1 and HDAC1 expression plays a vital role in the poor response of CRC to ubenimex.
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http://dx.doi.org/10.1038/s41419-021-03814-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144210PMC
May 2021

Associations between risk factors for cardiovascular diseases and frailty among community-dwelling older adults in Lanzhou, China.

Int J Nurs Sci 2021 Apr 25;8(2):168-174. Epub 2021 Mar 25.

School of Nursing, Lanzhou University, Lanzhou, China.

Objectives: To examine the relationship between cardiovascular disease risk factors and frailty in a sample of older Chinese adults.

Methods: A total of 458 community-dwelling older adults (≥65 years) in Lanzhou, Gansu Province of China participated in a cross-sectional survey. Their status was evaluated in terms of frailty phenotype (unintentional weight loss, exhaustion, low activity levels, slowness and weakness). Participants were categorized as not frail, prefrail or frail. Cardiovascular disease risk factors that were assessed included: blood pressure, body mass index, waist circumference, blood glucose, total cholesterol, triglycerides, low-density lipoproteins and high-density lipoproteins.

Results: Individuals with obesity had an increased risk of prefrailty (: 2.26; 95% CI: 1.05, 4.84). Hypertension was inversely associated with frailty among the participants (: 0.31; 95% CI: 0.11, 0.87) after adjusting for covariates.

Conclusions: The findings suggest that much more attention should be paid to weight control of the elderly in the community for preventing them from transition to prefrailty or frailty. Active prevention and control of cardiovascular diseases among the community-dwelling elder are still of great importance.
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http://dx.doi.org/10.1016/j.ijnss.2021.03.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105554PMC
April 2021

Epimutation of MMACHC compound to a genetic mutation in cblC cases.

Mol Genet Genomic Med 2021 Jun 12;9(6):e1625. Epub 2021 May 12.

Department of Pediatric Endocrinology and Genetic Metabolism, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, Zhengzhou, China.

Background: Methylmalonic aciduria (MMA) combined with homocystinuria, cobalamin(cbl)C deficiency type (OMIM 277400), is the most common autosomal recessive inherited disorder of intracellular cobalamin metabolism caused by mutations in the MMACHC gene (OMIM 609831), of which more than 100 mutations have been identified to date. In this study, we only identified a coding mutation in one allele at the MMACHC gene locus, and no large fragments deletion or duplication were found. Up to now, only three epimutation cblC cases were reported. We hypothesized whether the MMACHC was hypermethylated.

Methods: To address this hypothesis, the entire coding region and adjacent splice sites of the panel genes involved in metabolic diseases were sequenced using the Illumina HiSeq X platform, followed by confirmation via Sanger sequencing in their parents and brothers. Methylation analysis of the MMACHC was performed using an EpiTect Bisulfite Kit and methylation-specific PCR (MSP) to investigate the role of epimutations in cblC disease.

Results: We identified a clearly pathogenic single heterozygous c.658_660del, p. (K220del) mutation, which was also identified in the mother. Analysis of the MMACHC indicated a heterozygous epimutation consisting of 34 hypermethylated CpG sites in a CpG island encompassing the promoter and first exon of the MMACHC, which was also identified in the father. Furthermore, we identified a single heterozygous c.*2C>T mutation in the sixth exon of the PRDX1 (OMIM 176763) in patients and their fathers, which was the only sequence variation that segregated with the MMACHC methylation. Neither c.658_660del and epimutation in MMACHC nor c.*2C>T in PRDX1 was discovered in her brother.

Conclusion: We report compound heterozygotes in MMACHC for a genetic mutation and an epimutation in cblC cases. To our best knowledge, this is the first report of two cblC cases from China caused by compound heterozygous mutations with a coding mutation in one allele and an epimutation in the other at the MMACHC locus.
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http://dx.doi.org/10.1002/mgg3.1625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222841PMC
June 2021

Epimutation of MMACHC compound to a genetic mutation in cblC cases.

Mol Genet Genomic Med 2021 Jun 12;9(6):e1625. Epub 2021 May 12.

Department of Pediatric Endocrinology and Genetic Metabolism, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, Zhengzhou, China.

Background: Methylmalonic aciduria (MMA) combined with homocystinuria, cobalamin(cbl)C deficiency type (OMIM 277400), is the most common autosomal recessive inherited disorder of intracellular cobalamin metabolism caused by mutations in the MMACHC gene (OMIM 609831), of which more than 100 mutations have been identified to date. In this study, we only identified a coding mutation in one allele at the MMACHC gene locus, and no large fragments deletion or duplication were found. Up to now, only three epimutation cblC cases were reported. We hypothesized whether the MMACHC was hypermethylated.

Methods: To address this hypothesis, the entire coding region and adjacent splice sites of the panel genes involved in metabolic diseases were sequenced using the Illumina HiSeq X platform, followed by confirmation via Sanger sequencing in their parents and brothers. Methylation analysis of the MMACHC was performed using an EpiTect Bisulfite Kit and methylation-specific PCR (MSP) to investigate the role of epimutations in cblC disease.

Results: We identified a clearly pathogenic single heterozygous c.658_660del, p. (K220del) mutation, which was also identified in the mother. Analysis of the MMACHC indicated a heterozygous epimutation consisting of 34 hypermethylated CpG sites in a CpG island encompassing the promoter and first exon of the MMACHC, which was also identified in the father. Furthermore, we identified a single heterozygous c.*2C>T mutation in the sixth exon of the PRDX1 (OMIM 176763) in patients and their fathers, which was the only sequence variation that segregated with the MMACHC methylation. Neither c.658_660del and epimutation in MMACHC nor c.*2C>T in PRDX1 was discovered in her brother.

Conclusion: We report compound heterozygotes in MMACHC for a genetic mutation and an epimutation in cblC cases. To our best knowledge, this is the first report of two cblC cases from China caused by compound heterozygous mutations with a coding mutation in one allele and an epimutation in the other at the MMACHC locus.
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http://dx.doi.org/10.1002/mgg3.1625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222841PMC
June 2021

Clinical characteristics of axial spondyloarthritis patients in China: results from ChinaSpA, the Chinese Spondyloarthritis Registry.

Clin Exp Rheumatol 2021 Apr 23. Epub 2021 Apr 23.

Department of Rheumatology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences; Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education; National Clinical Research Center for Dermatologic and Immunologic Diseases, Beijing, China.

Objectives: The objective of this study is to describe the clinical features of patients with axial spondyloarthritis (axial SpA) in the ChinaSpA registry.

Methods: Patients with clinical diagnosis of ankylosing spondylitis (AS) or axial SpA were enrolled into the registry. Patients with a complete set of pelvis radiograph, pelvis MRI and HLA-B27 (Complete Set group, CS group) were further categorised based on classification criteria into AS, radiographic axial SpA (r-axSpA) and non-radiographic axial SpA (nr-axSpA). Early axial SpA was defined as symptom duration of less than three years. Descriptive statistics were used to describe clinical characteristics of enrolled patients. ANOVA analyses were used to compare patients in different groups.

Results: A total of 5270 patients were enrolled in the study, and 3223 patients had complete sets of pelvis radiographs, MRIs and HLA-B27 status. Among them, more than 80% patients met both the ASAS criteria for r-axSpA and the modified New York criteria for AS. Among those with early axial SpA, 92% of patients had sacroiliitis on pelvis radiograph, 3.8% had sacroiliitis only on pelvis MRI, and 3.8% were in the clinical arm without any sacroiliitis on imaging studies. Patients in nr-axSpA clinical arm had less diagnosis delay, lower inflammatory markers and ASDAS, compared topatients in the r-axSpA, nr-axSpA MRI arm.

Conclusions: In the ChinaSpA registry, patients in nr-axSpA clinical arm had the shortest diagnostic delay, lower inflammatory markers and ASDAS, but no difference in extra-articular manifestation, compared to patients in the r-axSpA and nr-axSpA MRI arm.
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April 2021

Improved Tolerance of Mycorrhizal Seedlings to Sulfuric Acid Rain Related to Phosphorus and Zinc Contents in Shoots.

J Fungi (Basel) 2021 Apr 14;7(4). Epub 2021 Apr 14.

State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, China.

Acid rain (AR) is an increasingly serious environmental problem that frequently occurs in Southern China with sulfuric acid rain (SAR) as the main type. SAR can negatively affect the growth and physiological properties of trees, but mycorrhizal associations may mitigate such detrimental effects. However, the mechanisms by which arbuscular mycorrhizal fungi control SAR-induced impacts on plants remain unclear. A pot experiment was conducted on seedlings, an economically important tree species in Southern China, in which inoculated and non-inoculated seedlings were subjected to three simulated SAR regimes (pH of 5.6, 4.0, and 2.5, respectively) to examine the effects on the growth, osmotic regulation, and nutrient absorption of these seedlings. The results show that, although SAR had no effect on the accumulation of biomass, it significantly decreased the concentrations of proline and soluble protein, shoot Zn, P, K, and Ca concentrations, and the Fe and Mn concentrations of shoots and roots. Mycorrhizal inoculation, especially with , significantly increased total biomass, proline concentration, and the Zn, P, and K concentrations in the shoots of under lower pH conditions. Moreover, our findings suggest that the combination of root colonization, acid tolerance, and the concentrations of shoot-P, shoot-Zn, and root-Fe of jointly conferred mycorrhizal benefits on the plants under SAR conditions. Given the enhancement of the nutritional quality of owing to mycorrhizal associations, inoculation with may be preferable for the culturing and management of these plants under acidic conditions.
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http://dx.doi.org/10.3390/jof7040296DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070988PMC
April 2021

Dosimetric effects of supine immobilization devices on the skin in intensity-modulated radiation therapy for breast cancer: a retrospective study.

BMC Cancer 2021 Apr 9;21(1):384. Epub 2021 Apr 9.

Second Affiliated Hospital of Fujian Medical University, NO 950, Donghai Street, Fengze District, Quanzhou, 362000, Fujian, China.

Background: The dose perturbation effect of immobilization devices is often overlooked in intensity-modulated radiation therapy (IMRT) for breast cancer (BC). This retrospective study assessed the dosimetric effects of supine immobilization devices on the skin using a commercial treatment planning system.

Methods: Forty women with BC were divided into four groups according to the type of primary surgery: groups A and B included patients with left and right BC, respectively, who received 50 Gy radiotherapy in 25 fractions after radical mastectomy, while groups C and D included patients with left and right BC, respectively, who received breast-conservation surgery (BCS) and 40.05 Gy in 15 fractions as well as a tumor bed simultaneous integrated boost to 45 Gy. A 0.2-cm thick skin contour and two sets of body contours were outlined for each patient. Dose calculations were conducted for the two sets of contours using the same plan. The dose differences were assessed by comparing the dose-volume histogram parameter results and by plan subtraction.

Results: The supine immobilization devices for BC resulted in significantly increased skin doses, which may ultimately lead to skin toxicity. The mean dose increased by approximately 0.5 and 0.45 Gy in groups A and B after radical mastectomy and by 2.7 and 3.25 Gy in groups C and D after BCS; in groups A-D, the percentages of total normal skin volume receiving equal to or greater than 5 Gy (V) increased by 0.54, 1.15, 2.67, and 1.94%, respectively, while the V increased by 1.27, 1.83, 1.36, and 2.88%; the V by 0.85, 1.87, 2.76, and 4.86%; the V by 1.3, 1.24, 10.58, and 11.91%; and the V by 1.29, 0.65, 10, and 10.51%. The dose encompassing the planning target volume and other organs at risk, showed little distinction between IMRT plans without and with consideration of immobilization devices.

Conclusions: The supine immobilization devices significantly increased the dose to the skin, especially for patients with BCS. Thus, immobilization devices should be included in the external contour to account for dose attenuation and skin dose increment.

Trial Registration: This study does not report on interventions in human health care.
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http://dx.doi.org/10.1186/s12885-021-08119-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034111PMC
April 2021

MiR-452 Regulates C2C12 Myoblast Proliferation and Differentiation Targeting .

Front Genet 2021 12;12:640807. Epub 2021 Mar 12.

Institute of Cellular and Molecular Biology, Jiangsu Normal University, Xuzhou, China.

microRNAs are a kind of endogenous, non-coding, single-strand small RNA. They have been reported as an important regulatory factor in skeletal myogenesis. In this study, miR-452 was selected from RNA high-throughput sequencing data to explore its regulatory role in myogenesis. Functionally, miR-452 overexpression could promote C2C12 myoblast proliferation while inhibiting myogenic differentiation. On the contrary, inhibition of miR-452 could suppress C2C12 myoblast proliferation but accelerate myogenic differentiation. Bioinformatics analysis and dual luciferase report assays showed that (), , and were the potential target genes of miR-452. To further confirm the target relationship between , , and with miR-452, the mRNA level and protein level of these genes were detected by using RT-qPCR and Western blot, respectively. Result analysis indicated that was a target gene of miR-452. In addition, knockdown of could obviously promote C2C12 myoblast proliferation but block their differentiation. In summary, these results demonstrated that miR-452 promoted C2C12 myoblast proliferation and inhibited their differentiation targeting .
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http://dx.doi.org/10.3389/fgene.2021.640807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994525PMC
March 2021

Validation of the REVEAL Prognostic Models in Systemic Lupus Erythematosus-Associated Pulmonary Arterial Hypertension.

Front Med (Lausanne) 2021 4;8:618486. Epub 2021 Mar 4.

Department of Epidemiology and Bio-Statistics, Institute of Basic Medical Sciences, China Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

No previous studies have investigated the predictive performance of the Registry to Evaluate Early and Long-term Pulmonary Arterial Hypertension Disease Management (REVEAL) prognostic equation and simplified risk score calculator in patients with systemic lupus erythematosus-associated pulmonary arterial hypertension (SLE-PAH). We aimed to validate these prediction tools in an external cohort of patients with SLE-PAH. In this study, the validation cohort consisted of patients with SLE-PAH registered in a prospective, multicenter, nationwide database between November 2006 and May2016. The follow-up of patients was censored at 1 year. Discrimination, calibration, model fit, and risk stratification of the REVEAL prognostic equation and simplified risk score calculator were validated. As a result, a total of 306 patients with SLE-PAH were included. The 1-year overall survival rate was 91.5%. The C-index of the prognostic equation was 0.736, demonstrating reasonably good discrimination, and it was greater than that for the simplified risk score calculator (0.710). The overall calibration slope was 0.83, and the Brier score was 0.079. The risk of renal insufficiency and World Health Organization Functional Class III (WHO FC III) were underestimated, and the risk assigned to a heart rate >92 bpm in the REVEAL prognostic models was not observed in our validation cohort. Both model discrimination and calibration were poor in the very high-risk group. In conclusion, the REVEAL models exhibit good discriminatory ability when predicting 1-year overall survival in patients with SLE-PAH. Findings from both models should be interpreted with caution in cases of renal insufficiency, WHO FC III, and heart rate >92 bpm.
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http://dx.doi.org/10.3389/fmed.2021.618486DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969505PMC
March 2021

Enhancing flotation separation of chalcopyrite and magnesium silicate minerals by surface synergism between PAAS and GA.

Sci Rep 2021 Mar 18;11(1):6368. Epub 2021 Mar 18.

Key Laboratory of Ministry of Education for Solid Waste Treatment and Resource Recycle, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China.

Separation effects of sodium polyacrylate (PAAS) and gum Arabic (GA) on flotation of chalcopyrite and magnesium silicate minerals using potassium butyl xanthate (PBX) as collector were investigated by micro-flotation experiments, zeta potential, Infrared spectral (IR), SEM-EDS, XPS analysis and copper sulphide ore beneficiation test. The micro-flotation experiments and zeta potential measurements showed that combined depressant consisting of PAAS and GA could efficiently reduce the recoveries of mixed minerals of serpentine and talc more than 25%, while that of chalcopyrite remained above 70% at pH 9.2. Infrared spectral (IR), SEM-EDS and XPS analysis showed that PAAS chemically reacted with Mg on the surface of serpentine, while GA adsorbed on talc surface mainly via physical interaction and hydrogen bond may also play a role. Surface synergism between PAAS and GA was investigated by turbidity test and its depression mechanism was proposed. The technology feasibility of using PAAS and GA to improve the copper sulphide ore flotation performance was verified through artificial mixed ore flotation and laboratory closed-flotation operation.
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http://dx.doi.org/10.1038/s41598-021-85984-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973509PMC
March 2021

sp. nov., isolated from a soil sample of Collins Glacier front, Antarctica.

Int J Syst Evol Microbiol 2019 Jun 16;71(3). Epub 2021 Mar 16.

China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.

A Gram-stain-negative, rod-shaped, and aerobic bacterium, strain 16-35-5, was isolated from Collins Glacier front soil from the Fildes Peninsula, Antarctica. The bacterium grew optimally at 28 °C, pH 7.0 and in the presence of 0-4.0 % (w/v) NaCl. On the basis of the results of 16S rRNA gene sequence phylogenetic analyses, it was concluded that 16-35-5 represented a member of the genus and had the highest sequence similarities with CFBP 3232 (96.48 %). The genome of 16-35-5 is 4.2 Mb long with a DNA G+C content of 66.3 mol%. Average nucleotide identity (ANI) value between the genomes of 16-35-5 and DSM 27981, was 85.29 %. Strain 16-35-5 had ubiquinone-8 (Q-8) as the respiratory ubiquinone. The polar lipids of 16-35-5 were consisted of phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The main fatty acids were summed feature 3 (Cω7 and/or Cω6, 25.2 %), summed feature 8 (Cω7 and/or Cω6, 12.9 %), C (35.2 %), and C cyclo (19.0 %). On the basis of the evidence presented in this study, 16-35-5 should be classified as representing a novel species of the genus , for which the name sp. nov., is proposed, with the type strain 16-35-5 (=CCTCC AB 2019325=KCTC 72915).
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http://dx.doi.org/10.1099/ijsem.0.004744DOI Listing
June 2019

Analytical comparability assessment on glycosylation of ziv-aflibercept and the biosimilar candidate.

Int J Biol Macromol 2021 Jun 6;180:494-509. Epub 2021 Mar 6.

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China. Electronic address:

Ziv-aflibercept (aflibercept) is a recombinant fusion protein which combines the portions of human vascular endothelial growth factor receptors extracellular domains fused to the Fc portion of human IgG1. It is a highly sialylated glycoprotein with 5 N-glycosylation sites. In this study, a comprehensive strategy for comparability study of the complex glycosylation was developed between aflibercept and the biosimilar candidate including the investigations on N-glycosylation sites, site occupancy, site-specific glycoforms, released glycans and sialic acids. The results indicated that same N-glycosylation sites were identified, site occupancy were 100% except N site, site-specific glycoforms and released glycans showed similar glycan species, contents of NANA were at a same level for two products. Minor differences were found between two products. The biosimilar candidate presented lower level of aglycosylation, lower level of glycans containing one terminal sialic acid, higher level of glycans containing two terminal sialic acids, higher level of G0F and Man5, lower level of G1F and G2F compared with aflibercept. However, further studies exhibited no differences were observed in the cell-based biological potency and Fc effector function. Moreover, the biosimilar candidate showed a similar pharmacokinetics curve and bioequivalence compared with aflibercept.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.03.020DOI Listing
June 2021

Increased aluminum and lithium and decreased zinc levels in plasma is related to cognitive impairment in workers at an aluminum factory in China: A cross-sectional study.

Ecotoxicol Environ Saf 2021 May 5;214:112110. Epub 2021 Mar 5.

Department of Occupational Health, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi 030001, China. Electronic address:

Background: Previous studies have shown that multiple imbalances of metal ions in the brain are closely associated with the neurodegenerative disorders. Our studies have shown that long-term working exposure to aluminum induces increased plasma aluminum levels and causes cognitive impairment in workers at aluminum factories.

Objective: To explore the levels of nine metals in plasma and the effect on cognitive function among in-service workers.

Methods: In this cross-sectional study, cognitive function was assessed using the Montreal Cognitive Assessment (MoCA), which included seven subitems: executive/visuospatial abilities; naming; attention and calculation; language; abstract; recall; and orientation. The plasma levels of nine kinds of metals were measured by inductively coupled plasma-mass spectrometry (ICP-MS). A multivariate generalized linear regression model and Bayesian kernel machine regression (BKMR) were selected to estimate the relationship between metal plasma level and MoCA scores with adjustment for confounders.

Results: One hundred and eighty-seven workers participated in this study. In the multivariable generalized linear model, among these nine metals studied, five were related to the MoCA score: aluminum, lithium, cobalt, zinc and chromium. In the BKMR model, a significantly negative correlation between the plasma aluminum, lithium and the total MoCA score was observed. Moreover, for subitems on the MoCA scale, the plasma levels of lithium, aluminum, and zinc had a significant correlation with the executive/visuospatial abilities, naming, and orientation abilities, respectively. The log-transformation concentrations of plasma aluminum and lithium were negatively correlated with the executive/visuospatial abilities and naming abilities, respectively. The log-transformation plasma zinc concentration was positively correlated with orientation abilities.

Conclusion: Based on the results, we determined that increased aluminum and lithium and decreased zinc levels in plasma were associated with the incidence of mild cognitive impairment (MCI) in workers at a Chinese aluminum plant.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112110DOI Listing
May 2021

Experimental study on renoprotective effect of intermedin on diabetic nephropathy.

Mol Cell Endocrinol 2021 05 4;528:111224. Epub 2021 Mar 4.

Department of Nephrology, Postdoctoral Workstation of Shanxi Provincial People's Hospital, The Affiliated People's Hospital of Shanxi Medical University, Shanxi Kidney Disease Institute, Taiyuan, Shanxi, 030012, China. Electronic address:

Intermedin(IMD) is a novel member of the calcitonin/calcitonin gene-related peptide (CT/CGRP) family that has anti-inflammatory, antioxidant and anti-apoptosis properties. This study aimed to evaluate the renoprotective effects of IMD on podocyte apoptotic loss and slit diaphragm protein deficiency the kidneys of rats with in streptozotocin (STZ) induced diabetes in high glucose-exposed podocytes. Our results showed that IMD significantly attenuated proteinuria, and alleviated the abnormal alterations in glomerular ultrastructure in vivo. IMD also improved the induction of slit diaphragm proteins, and restored the decreased Bcl-2 expression and suppressed Bax and caspase-3 induction in the diabetic glomeruli. In addition, IMD attenuated podocyte apoptosis and filamentous actin (F-actin) rearrangement in high glucose-exposed podocytes. Exposure to high glucose elevated the unfolded protein response (UPR) to endoplasmic reticulum (ER) stress in renal podocytes, and IMD treatment blocked such ER stress responses pertinent to podocyte apoptosis and reduced synthesis of slit diaphragm proteins in vivo and in vitro. These observations demonstrate that targeting ER stress is an underlying mechanism of IMD-mediated amelioration of diabetes-associated podocyte injury and dysfunction.
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http://dx.doi.org/10.1016/j.mce.2021.111224DOI Listing
May 2021

Epidemic patterns of antimicrobial resistance of Salmonella enterica serovar Gallinarum biovar Pullorum isolates in China during the past half-century.

Poult Sci 2021 Mar 10;100(3):100894. Epub 2020 Dec 10.

Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou, China. Electronic address:

Pullorum is a chicken-specific systemic disease caused by Salmonella enterica serovar Gallinarum biovar Pullorum (S. Pullorum). This study was carried out to provide basic data for understanding the trends of S. Pullorum. A total of 652 S. Pullorum isolates collected in China during 1962-2019 were examined. Overall, 525 (80.5%) isolates were resistant to at least one antibiotic; 280 (42.9%) isolates resisted 3 or more classes of antibiotics and showed an increasing trend until 2015 and then decreased significantly. The most common multidrug-resistant pattern was ampicillin-tetracycline-nalidixic acid (13.6%). After 2008, 6 classes of antibiotic-resistant strains began to appear, and they have been prevalent ever since. In 2014, a strain resistant to 7 antibiotics (ampicillin-cefazolin-streptomycin-tetracycline-sulphonamides-nalidixic acid-nitrofurantoin) was isolated. The highest antimicrobial resistance was observed for nalidixic acid (71.9%), and the lowest was found for cefotaxime, meropenem, amikacin, gentamicin, fosfomycin, and polymyxin (0%). Our findings monitored the prevalence of the resistance of S. Pullorum during the past half-century in China. Continued surveillance of antimicrobial resistance and the rational use of antimicrobials is necessary and important to control the rapid increase in antimicrobial resistance in S. Pullorum.
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http://dx.doi.org/10.1016/j.psj.2020.12.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936142PMC
March 2021

Regulation of ARL2 in colorectal cancer cell proliferation and tumorigenicity, and its negative association with AXL.

Oncol Lett 2021 Mar 11;21(3):196. Epub 2021 Jan 11.

Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215008, P.R. China.

Colorectal cancer (CRC) is the third most common malignant disease in adults. ADP ribosylation factor-like GTPase 2 (ARL2) is crucial for controlling the dynamics of microtubules and mitochondrial functions. However, the biological function of ARL2 in CRC remains unclear. The present study was performed to identify the expression level and functional role of ARL2 in CRC. A total of 19 CRC and 3 normal healthy colorectal tissues were collected. Furthermore, ARL2 expression was analyzed in healthy colorectal and CRC tissues by immunohistochemistry (IHC). ARL2 overexpression and knockdown was achieved using lentiviral vectors and plasmid transfection in HCT8 and HCT116 cells. The protein and mRNA expression levels of ARL2 and AXL were analyzed using western blot and reverse transcription-quantitative PCR in ARL2 knockdown and ARL2 overexpressing HCT8 and HCT116 cells. Cell Counting Kit-8, colony formation, wound healing, and Matrigel assays were used to investigate the biological functions of ARL2. Taken together, ARL2 protein expression level was upregulated in CRC tissues. Furthermore, ARL2 overexpression decreased proliferation and weakened the colony-formation abilities of the CRC cells, as well as their migratory and invasive abilities. ARL2 interference enhanced proliferation and colony-formation rates of the CRC cells, as well as their migratory and invasive abilities. ARL2 regulated CRC proliferation and tumorigenicity and was negatively associated with AXL. The results of the present study suggested that the proliferation, migration and tumorigenicity of the CRC cells could be inhibited by ARL2 overexpression. The latter may be used as a predicted and potential therapeutic target for CRC.
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http://dx.doi.org/10.3892/ol.2021.12457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816291PMC
March 2021

Controllable synthesis of Cu-Ni-M (M = S, P and Se) hybrid nanoarrays for efficient water splitting reaction.

Dalton Trans 2021 Mar;50(8):2964-2972

School of Science, North University of China, Taiyuan 030051, People's Republic of China.

Electrochemical water splitting has become one of the state of the art approaches to generate hydrogen. It is important to exploit relatively low toxicity, low cost and environmentally friendly water splitting electrocatalysts. A series of Cu-Ni-M (M = S, P and Se) materials were firstly in situ grown on Ni foam and these materials showed excellent water splitting activity. The Cu-Ni-S material shows excellent oxygen evolution reaction performance (200 [email protected] mA cm-2) and the Cu-Ni-P sample shows an effective hydrogen evolution reaction performance (52 [email protected] mA cm-2). When the Cu-Ni-S and Cu-Ni-P materials were assembled into a two-electrode system, the Cu-Ni-S/NF//Cu-Ni-P/NF electrode pairs display superior water splitting activity (1.50 [email protected] mA cm-2), which is one of the best electrocatalytic activities reported so far. The experimental analysis demonstrates that the excellent performance of the Cu-Ni-S/NF and Cu-Ni-P/NF materials is attributed to the rapid electron transfer rate, increased electrocatalytically active area, more exposure to active sites and the superior synergistic catalytic factor of Ni2+ and Cu2+. It was found that amorphous oxides were in situ generated on the outside surface of the catalyst through the analysis of the catalyst after the reaction, and they were the real electrocatalytically active centers. Density functional theory demonstrates that the in situ generated Cu-doped NiOOH shows the optimal water adsorption energy compared with NiOOH. This work offers novel views for the design of relatively low toxicity, stable and inexpensive water splitting electrocatalysts.
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http://dx.doi.org/10.1039/d0dt04179cDOI Listing
March 2021

Clinical Features and Outcomes of Neuropsychiatric Systemic Lupus Erythematosus in China.

J Immunol Res 2021 18;2021:1349042. Epub 2021 Jan 18.

Department of Rheumatology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, National Clinical Research Center for Dermatologic and Immunologic Diseases, Ministry of Science & Technology, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, No. 1 Shuaifuyuan, Beijing 100730, China.

Objective: To identify the clinical characteristics, magnetic resonance imaging (MRI) results, and prognostic factors of neuropsychiatric (NP) systemic lupus erythematosus (SLE; NPSLE) in a relatively large patient series in China.

Methods: Data of patients with NPSLE at Peking Union Medical College Hospital (PUMCH) were collected retrospectively from June 2012 to June 2016. NPSLE patients were compared with 220 non-NPSLE patients. Survival rates were evaluated using the Kaplan-Meier curves, log-rank test, and Cox proportional hazards modeling. Cranial MRI results were also studied.

Results: Of the 194 included patients, sixteen subtypes of NPSLE were identified, and the most common manifestations were seizure (36.6%), acute confusional state (25.3%), and cerebral vascular disease (15.5%). Compared with the non-NPSLE group, NPSLE patients were significantly more likely to have typical lupus symptoms, higher Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2K) scores ( = 0.002), and positive rate of anti-ribosomal P protein antibodies ( = 0.008). Patients with seizure were more likely to have higher SLEDAI-2K scores and positive anti-2GP1 than non-NPSLE patients. Sixteen patients died during follow-up. The most common cause of death was infection (37.5%). NPSLE significantly decreased survival rates of SLE patients. Patients with elevated serum creatinine ( = 0.001), hypocomplementemia ( = 0.031), and SLEDAI - 2K scores ≥ 15 ( = 0.014) had shorter survival periods. Eighty-two patients underwent detailed cranial MRI analysis; of these, 50 (61.0%) had abnormal results. Small vessel disease was the most common abnormal finding, followed by inflammatory-like lesions and large vessel disease.

Conclusions: High disease activity and positive rate of anti-ribosomal P protein antibodies may be risk factors for NPSLE. NPSLE decreases survival rates of SLE patients. Renal insufficiency and high disease activity are predictive of poor prognoses for NPSLE patients.
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http://dx.doi.org/10.1155/2021/1349042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7834780PMC
January 2021

Paeoniflorin Sensitizes Breast Cancer Cells to Tamoxifen by Downregulating microRNA-15b via the FOXO1/CCND1/β-Catenin Axis.

Drug Des Devel Ther 2021 22;15:245-257. Epub 2021 Jan 22.

Department of Traditional Chinese Medicine, Kunming Second People's Hospital, Kunming, Yunnan, 650000 People's Republic of China.

Background: Paeoniflorin (Pae) possesses anti-tumor activity in various malignancies. However, it is unclear whether Pae plays a sensitizer role in breast cancer (BC) and the molecular mechanisms involved in this process. Our oligonucleotide microarray revealed that microRNA (miR)-15b is the most significantly downregulated miRNA in MCF-7/4-hydroxytamoxifen (4-OHT) cells treated with Pae. This paper summarized the relevance of Pae in BC cell endocrine resistance to tamoxifen (Tam) and the molecular mechanisms involved miR-15b expression.

Materials And Methods: 4-OHT-resistant BC cell lines were developed and treated with different concentrations of Pae. Flow cytometry, lactose dehydrogenase activity, caspase-3 activity, colony formation, and EdU assays were carried out to assess the impact of Pae on BC cells. Differentially expressed miRNAs in BC cells treated with Pae were analyzed by microarray. Targeting mRNAs of screened miR-15b as well as the binding of forkhead box O1 (FOXO1) to the cyclin D1 (CCND1) promoter sequence were predicted through bioinformatics analysis. Finally, the expression of β-catenin signaling-related genes in cells was detected by Western blotting.

Results: Pae (100 μg/mL) inhibited the clonality and viability of BC cells, while enhancing apoptosis in vitro. Pae also repressed miR-15b expression. Overexpression of miR-15b restored the growth and resistance of BC cells to 4-OHT. Moreover, Pae promoted FOXO1 expression by downregulating miR-15b, thereby transcriptionally inhibiting CCND1 and subsequently blocking β-catenin signaling.

Conclusion: Pae inhibits 4-OHT resistance in BC cells by regulating the miR-15b/FOXO1/CCND1/β-catenin pathway.
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http://dx.doi.org/10.2147/DDDT.S278002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837563PMC
January 2021

Impacts of frailty on health care costs among community-dwelling older adults: A meta-analysis of cohort studies.

Arch Gerontol Geriatr 2021 May-Jun;94:104344. Epub 2021 Jan 20.

Reproductive Medicine Department of the First Affiliated Hospital of Lanzhou University, NO.1 Donggang West Road, Lanzhou, Gansu 730000, PR China; Key laboratory of reproductive medicine and embryology gansu province, NO.1 Donggang West Road, Lanzhou, Gansu 730000, PR China. Electronic address:

Background: The demands for health care services from the frail elderly individuals in the community continue to increase, which will exert a tremendous burden on health care costs. However, little is known regarding the magnitude of these impacts. In this study, we performed a systematic review and meta-analysis of the evidence to explore the impact of frailty on health care costs among community-dwelling older adults.

Materials And Methods: Relevant published articles were searched from PubMed, Embase, Web of Science, the Cochrane Library, China National Knowledge Infrastructure (CNKI), Chinese Science and Technology Journal Full-text Database (VIP), Wanfang Database, Chinese Biomedical Literature Database (CBM), and the reference lists of articles. Published cohort or cross-sectional studies assessing the impacts of frailty on health care costs among community-dwelling older adults were identified (to June 2020). The outcomes on health care costs before and after baseline were stratified by frailty status.

Results: A total of 7 cohort studies comprised of a total of 3,750,611 participants were included in our study. Our analyses showed that: (1) compared with the robust group, health care costs increased by $79-13,423.83 (standardized mean difference, SMD = 0.22, 95% Confidence interval, 95% CI, 0.22-0.22; P < 0.00001) in the pre-frail elderly and by $616-32,549.96 (SMD = 0.55, 95% CI, 0.44-0.67; P < 0.00001) in the frail elderly in the community. A significantly higher in the increase of health care costs was observed in the frail group compared with the pre-frail group(SMD = 0.35, 95% CI, 0.19-0.51; P < 0.0001); (2) the frailty phenotype components increased the health care costs of the elderly in community (weight loss: $1,630-6,209, SMD = 0.43, 95% CI, 0.17-0.69; P = 0.001; weakness: $275-7,586, SMD = 0.24, 95% CI, 0.08-0.40; P = 0.001; exhaustion: $1,545-10,559, SMD = 0.31, 95% CI, 0.13-0.49; P = 0.0006; slowness: $352-1,1891, SMD = 0.40, 95% CI = 0.14-0.65; P = 0.003; low physical activity: $512-3,459, SMD = 0.26, 95% CI, 0.16-0.36; P < 0.00001); (3) the increase in the frailty index was parallel with the increase in health care costs by $12,363-21,066 (SMD = 0.41, 95% CI, 0.29-0.53; P < 0.00001).

Conclusions And Implications: This study revealed the adverse economic impacts of frailty status, frailty phenotype components, and frailty index on health care costs in community-dwelling older adults. Future research is warranted to investigate costs incurred by interventions to improve frailty, which will provide further insights into additional health care costs due to frailty.
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http://dx.doi.org/10.1016/j.archger.2021.104344DOI Listing
May 2021

Effects of different dissolved organic matter on microbial communities and arsenic mobilization in aquifers.

J Hazard Mater 2021 06 14;411:125146. Epub 2021 Jan 14.

State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China; School of Environmental Studies, China University of Geosciences, Wuhan 430074, PR China.

Dissolved organic matter (DOM) play key roles in the biotransformation of arsenic in groundwater systems. However, the effects of different types of DOM on arsenic biogeochemistry remain poorly understood. In this study, four typical DOM compounds (acetate, lactate, AQS and humic acid) were amended to high As aquifer sediments to investigate their effects on arsenic/iron biotransformation and microbial community response. Results demonstrated that different DOM drove different microbial community shifts and then enhanced microbially-mediated arsenic release and iron reduction. With labile DOM (acetate and lactate) amendment, the abundance of putative dissimilatory iron and sulfate reducers Desulfomicrobium and Clostridium sensu stricto increased within the first week, and subsequently the anaerobic fermentative bacterial genus Acetobacterium and arsenate/sulfate-reducing bacterial genus Fusibacter became predominant. In contrast, recalcitrant DOM (AQS and humic acid) mainly stimulated the abundances of sulfur compounds respiratory genus Desulfomicrobium and fermentative bacterial genus Alkalibacter in the whole incubation. Accompanied with the microbial community structure and function shifts, dissolved organic carbon concentration and oxidation-reduction potential changed and the arsenic/iron reduction increased, which resulted in the enhanced arsenic mobilization. Collectively, the present study linked DOM type to microbial community structure and explored the potential roles of different DOM on arsenic biotransformation in aquifers.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125146DOI Listing
June 2021

The complete plastid genome of (Moraceae).

Mitochondrial DNA B Resour 2020 Sep 21;5(3):3335-3336. Epub 2020 Sep 21.

Center of Laboratory Equipment Management, Heilongjiang Bayi Agricultural University, Heilongjiang, China.

is a wild relative of which is an important economic plant. Here, we determined the complete plastid genome of using the Illumina paired reads to provide genomic feature resources. The whole plastid genome of is 160,603 bp in length, containing two inverted repeats (IRs) of 25,899 bp separated by a large single-copy (LSC 88,640 bp) region and a small single-copy (SSC 20,165 bp) region. The complete plastome sequence of will provide a useful resource for the evolutionary biology study as well as for the phylogenetic studies.
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http://dx.doi.org/10.1080/23802359.2020.1768914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783041PMC
September 2020

The recent progress of synergistic supramolecular polymers: preparation, properties and applications.

Chem Commun (Camb) 2021 Feb;57(12):1413-1429

Integrated Composites Laboratory (ICL), Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN 37996, USA.

Supramolecular polymers have the combined properties of both traditional polymers and supramolecules. They are generally formed via the self-assembled polymerization driven noncovalent interactions such as hydrogen bonding, π-π stacking, metal coordination, and host-guest interaction between building blocks. The driving force for the formation of supramolecular polymers has changed from single noncovalent interactions to multiple noncovalent interactions. The advantages of multiple noncovalent interactions driving the formation of supramolecular polymers are reviewed from four aspects: polymer construction, the enhancement of bonding strength, properties and topological structure. The applications are illustrated with detailed examples including self-healing, drug delivery, bioimaging, biomedicine, environmental sensing and electronics.
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http://dx.doi.org/10.1039/d0cc07247hDOI Listing
February 2021
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