Publications by authors named "Yanhong Li"

425 Publications

Intramuscular adipocyte and fatty acid differences between high-fat and control rabbit groups subject to a restricted diet.

Vet Med Sci 2021 Jul 17. Epub 2021 Jul 17.

College of Animal Science and Technology, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan, P. R. China.

Fatty acids of intramuscular fat (IMF) in rabbits can influence meat quality, but it is unclear which fatty acids benefit to human health. A rabbit model of weight gain and weight loss was constructed using two rabbit groups and two growth stages. Stage 1 included control group1 fed a commercial diet(CG1) and experimental group1 fed a high fat diet (EG1). Stage 2 include control group2(CG2) and experimental group2 (EG2) both fed a restricted commercial diet. We detected differences in blood biochemical indicators as well as changes in intramuscular adipose cells and intramuscular fatty acid content in control and experiment groups at two stages. High fat induction can make rabbits become obese, have higher concentrations of glucose (GLU), total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C) and free fatty acid (FFA), and lower concentrations of insulin (INS). In addition, a high-fat diet promotes hypertrophy of precursor adipocytes in femoral muscles. Conversely, a restricted diet causes weight loss, decreases the concentration of TG, FFA, and INS in CG2 and EG2, and increases the deposition of unsaturated fatty acids in the femoral muscle. The content of monounsaturated trans oleic acid (C18:1n-9T) in EG2 was significantly higher than in CG2, whereas oleic acid (C18:1n-9C) was significantly lower in EG2 than in CG2. The polyunsaturated fatty acids Linolenate (C18:3 n-3) and cis-5,8,11,14,17-Eicosapentaenoate (C20:5 n-3) increased in CG2 and EG2. The content of Linoleate (C18:2 n-6) and γ-Linolenic acid (C18:3 n-6) significantly increased in CG2. The content of cis-11,14-Eicosatrienoic acid (C20:2) decreased significantly in CG2, but increased significantly in EG2.Thus, a high-fat diet can increase the formation of unhealthy fatty acids. Conversely, weight loss due to a restricted diet leads to an increase in unsaturated fatty acids in the femoral muscle, indicating that it reduces obesity symptoms and it may improve meat quality in rabbit.
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http://dx.doi.org/10.1002/vms3.576DOI Listing
July 2021

Chemoradiation treatment patterns among United States Veteran Health Administration patients with unresectable stage III non-small cell lung cancer.

BMC Cancer 2021 Jul 16;21(1):824. Epub 2021 Jul 16.

Duke Clinical Research Institute, Duke University School of Medicine, Durham, NC, USA.

Background: The Veterans Health Administration (VHA) is the largest integrated health care system in the United States (US). Among VHA patients, the rate of use of concurrent chemoradiation therapy (CCRT) among those with unresectable, stage III non-small cell lung cancer (NSCLC) is unknown. The objective was to report recent CCRT treatment patterns in VHA patients and identify characteristics associated with receipt of CCRT.

Methods: Using Department of Veteran Affairs (VA) Cancer Registry System data linked to VA electronic medical records, we determined rates of CCRT, sequential CRT (SCRT), radiation therapy (RT) only, chemotherapy (CT) only, and neither treatment.

Results: Among 4054 VHA patients who met study criteria, CCRT rates slightly increased from 44 to 50% between 2013 and 2017. Factors associated with decreased odds of CCRT receipt compared to any other treatment included increasing age (adjusted odds ratio [aOR] per 10 years = 0.67; 95% CI: 0.60-0.76) and Charlson-Deyo comorbidity score (aOR = 0.94; 95% CI: 0.91-0.97). White race was associated with increased odds of CCRT receipt (aOR = 1.24; 95% CI: 1.004-1.53). In a chart review sample of 200 patients, less than half (n = 85) had a documented reason for not receiving CCRT. Among these, 29% declined treatment, and 71% did not receive CCRT due to "not being a candidate" for reasons related to frailty or lung nodules being too far apart for radiation therapy.

Conclusions: CCRT rates among VHA patients with unresectable, stage III NSCLC slightly increased from 2013 to 2017; however in 2017, only half were receiving CCRT. Older patients and those with multiple comorbidities were less likely to receive CCRT and even when controlling for these factors, non-white patients were less likely to receive CCRT.
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http://dx.doi.org/10.1186/s12885-021-08577-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285779PMC
July 2021

Therapeutic potential of human umbilical cord mesenchymal stem cells on aortic atherosclerotic plaque in a high-fat diet rabbit model.

Stem Cell Res Ther 2021 Jul 15;12(1):407. Epub 2021 Jul 15.

Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, CAMS&PUMC; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing, 100021, China.

Background: Atherosclerosis (AS) is a complex disease caused in part by dyslipidemia and chronic inflammation. AS is associated with serious cardiovascular disease and remains the leading cause of mortality worldwide. Mesenchymal stem cells (MSCs) have evolved as an attractive therapeutic agent in various diseases including AS. Human umbilical cord MSCs (UCSCs) have been used in cell therapy trials due to their ability to differentiate and proliferate. The present study aimed to investigate the effect of UCSCs treatment on atherosclerotic plaque formation and the progression of lesions in a high-fat diet rabbit model.

Methods: Rabbits were fed a high-fat diet and then randomly divided into three groups: control, model, and treatment groups. Rabbits in the treatment group were injected with UCSCs (6 × 10 in 500 μL phosphate buffered saline) after 1 month of high-fat diet, once every 2 weeks, for 3 months. The model group was given PBS only. We analyzed serum biomarkers, used ultrasound and histopathology to detect arterial plaques and laser Doppler imaging to measure peripheral blood vessel blood filling, and analyzed the intestinal flora and metabolism.

Results: Histological analysis showed that the aortic plaque area was significantly reduced in the treatment group. We also found a significant decrease in macrophage accumulation and apoptosis, an increase in expression of scavenger receptors CD36 and SRA1, a decrease in uptake of modified low-density protein (ox-LDL), and a decrease in levels of pro-inflammatory cytokines interleukin (IL)-6 and tumor necrosis factor (TNF)-α following UCSCs treatment. We also found that anti-inflammatory cytokines IL-10 and transforming growth factor (TGF)-β expression increased in the aorta atherosclerotic plaque of the treatment group. UCSCs treatment improved the early peripheral blood filling, reduced the serum lipid level, and inhibited inflammation progression by regulating the intestinal flora dysbiosis caused by the high-fat diet. More specifically, levels of the microbiota-dependent metabolite trimethylamine-N-oxide (TMAO) were down-regulated in the treatment group.

Conclusions: UCSCs treatment alleviated atherosclerotic plaque burden by reducing inflammation, regulating the intestinal flora and TMAO levels, and repairing the damaged endothelium.
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http://dx.doi.org/10.1186/s13287-021-02490-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281645PMC
July 2021

Aldosterone signaling defect in young infants: single-center report and review.

BMC Endocr Disord 2021 Jul 9;21(1):149. Epub 2021 Jul 9.

Department of Pediatrics, The First affiliated Hospital, Sun Yat-Sen University, No. 58, Zhongshan II Rd, Guangzhou, 510080, P. R. China.

Background: Aldosterone (Ald) is a crucial factor in maintaining electrolyte and water homeostasis. Defect in either its synthesis or function causes salt wasting (SW) manifestation. This disease group is rare, while most reported cases are sporadic. This study aimed to obtain an overview of the etiology and clinical picture of patients with the above condition and report our rare cases.

Methods: A combination of retrospective review and case studies was conducted at the Pediatric Endocrine unit of The First Affiliated Hospital Sun Yat Sen University from September 1989 to June 2020.

Results: A total of 187 patients with SW were enrolled, of which 90.4% (n = 169) were diagnosed with congenital adrenal hyperplasia (CAH). SW type 21-hydroxylase deficiency accounted for 98.8% (n = 167) of CAH diagnosis, while 1.2% (n = 2) was of lipoid CAH. Non-CAH comprised 9.6% (n = 18) of the total patients whose etiologies included SF-1 gene mutation (n = 1), X-linked adrenal hypoplasia congenita (n = 9), aldosterone synthase deficiency (ASD, n = 4), and pseudo-hypoaldosteronism type 1 (PHA1, n = 1). Etiologies were not identified in three patients. All of patients with ASD and PHA1 exhibited SW syndrome in their early neonatal period. DNA sequencing showed mutations of CYP11B2 for P1-P4 and NR3C2 for P5. P1 and P2 were sibling brothers affected by compound heterozygous mutations of c.1121G > A (p.R374Q) and c.1486delC p.(L496fs); likewise, P4 was identified with compound heterozygous mutations of c.1200 + 1G > A and c.240-1 G > T; meanwhile P3 demonstrated c.1303G > A p.(G435S) homozygous mutation in CYP11B2 gene. Lastly, P5 showed c.1768 C > T p.(R590*) heterozygous mutation in the NR3C2 gene.

Conclusion: Etiology of infant with aldosterone defect was mostly congenital. Renal and adrenal imaging are recommended to exclude renal causes. If clinical picture is suggestive, normal plasma Ald in early infancy cannot rule out aldosterone insufficiency.
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http://dx.doi.org/10.1186/s12902-021-00811-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272273PMC
July 2021

High-sensitivity and wide-temperature-range dual-mode optical thermometry under dual-wavelength excitation in a novel double perovskite tellurate oxide.

Dalton Trans 2021 Jul 6. Epub 2021 Jul 6.

School of Science, Chongqing University of Posts and Telecommunications, Chongqing 400065, P. R. China.

Novel double perovskite SrLaLiTeO6 (abbreviated as SLLT):Mn4+,Dy3+ phosphors synthesized using a solid-state reaction strategy exhibit distinct dual-emission of Mn4+ and Dy3+. High-sensitivity and wide-temperature-range dual-mode optical thermometry was exploited taking advantage of the diverse thermal quenching between Mn4+ and Dy3+ and the decay lifetime of Mn4+. The thermometric properties in the range of 298-673 K were investigated by utilizing the fluorescence intensity ratio (FIR) of Dy3+ (4F9/2 → 6H13/2)/Mn4+ (2Eg → 4A2g) and the Mn4+ (2Eg → 4A2g) lifetime under 351 nm and 453 nm excitation, respectively. The maximum relative sensitivities (SR) of the resultant SLLT:1.2%Mn4+,7%Dy3+ phosphor under 351 nm and 453 nm excitation employing the FIR technology were determined to be 1.60% K-1 at 673 K and 1.44% K-1 at 673 K, respectively. Additionally, the maximum SR values based on the lifetime-mode were 1.59% K-1 at 673 K and 2.18% K-1 at 673 K, respectively. It is noteworthy that the SR values can be manipulated by different excitation wavelengths and multi-modal optical thermometry. These results suggest that the SLLT:Mn4+,Dy3+ phosphor has prospective potential in optical thermometry and provide conducive guidance for designing high-sensitivity multi-modal optical thermometers.
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http://dx.doi.org/10.1039/d1dt01147bDOI Listing
July 2021

Correction to: Transmission of H7N9 influenza virus in mice by different infective routes.

Virol J 2021 Jul 6;18(1):140. Epub 2021 Jul 6.

Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center, Peking Union Medical Collage (PUMC); Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, No. 5 Pan Jia Yuan Nan Li, Chaoyang District, Beijing, 100021, China.

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http://dx.doi.org/10.1186/s12985-021-01603-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261966PMC
July 2021

Effectiveness of omalizumab in patients with severe allergic asthma: A retrospective study in China.

Respir Med 2021 Jun 29;186:106522. Epub 2021 Jun 29.

Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Background: We conducted the first real-world study of treatment with omalizumab, a humanized monoclonal anti-immunoglobulin E antibody, in Chinese patients with severe allergic asthma.

Objective: The primary objective was the steroid-sparing effect of omalizumab after 12 and 16 weeks of treatment. Characteristics of the patient population, treatment patterns, response rate, and other measures of therapeutic effectiveness were also reported.

Methods: This nationwide, retrospective, real-world study was conducted in patients with severe allergic asthma who were treated with omalizumab in China. Data, including demographics, Asthma Control Test (ACT) and laboratory and lung function test results, and omalizumab use information, were extracted from patient records collected as part of a previously conducted real-world survey (Asthma Group of the Respiratory Disease Society of the Chinese Medical Association).

Results: In total, 139 patient records were included; 131 and 118 patients remained on treatment at the ≥12- and ≥16-week time points, respectively. The mean ± standard deviation age and median asthma duration (interquartile range) were 47.4 ± 14.3 and 7 (4, 15) years, respectively; 75.6% of patients had a history of allergic disease. Reductions (versus baseline) in inhaled corticosteroid/long-acting β2 agonists or oral corticosteroids were reported in 61.1% and 63.6% of patients at ≥12 and ≥ 16 weeks, respectively. There were significant improvements in ACT scores (6.08, P < .001) and nitric oxide fraction in exhaled air (-13.0, P = .01) from baseline. Multivariate analysis revealed that age and allergic medical history were predictors of omalizumab treatment response. No serious adverse events were reported.

Conclusion: Real-world omalizumab treatment was efficacious and well-tolerated in Chinese patients with severe allergic asthma.
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http://dx.doi.org/10.1016/j.rmed.2021.106522DOI Listing
June 2021

MiR-208b Regulates Rabbit Preadipocyte Proliferation and Differentiation.

Genes (Basel) 2021 Jun 9;12(6). Epub 2021 Jun 9.

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

microRNAs (miRNAs) play an important role in gene regulation in animals by pairing with target gene mRNA. Many miRNAs are differentially expressed in the adipose tissue, often with conserved expression. In our study, we found that miR-208b expression was observed differently in the preadipocyte differentiation model. When miR-208b was overexpressed in the preadipocyte differentiation model, the overexpressed group displayed higher expression of and -the markers of preadipocyte differentiation. Oil Red O staining revealed that the count of lipid droplets was increased in the overexpressed group. When the expression of miR-208b was inhibited, the above indicators showed an opposite trend. Moreover, results from both 5-ethynyl-2'-deoxyuridine (EDU) and cell counting kit (CCK) analysis showed that miR-208b promoted the proliferation of preadipocyte. Expression of gene , a direct miR-208b target, was downregulated in the overexpressed group, providing a possible link to multiple signal pathways. Overall, our data indicate that miR-208b play a positive regulatory effect on the proliferation and differentiation of rabbit preadipocyte.
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http://dx.doi.org/10.3390/genes12060890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228405PMC
June 2021

Untargeted Metabolomic Characteristics of Skeletal Muscle Dysfunction in Rabbits Induced by a High Fat Diet.

Animals (Basel) 2021 Jun 9;11(6). Epub 2021 Jun 9.

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

Type 2 diabetes and metabolic syndrome caused by a high fat diet (HFD) have become public health problems worldwide. These diseases are characterized by the oxidation of skeletal muscle mitochondria and disruption of insulin resistance, but the mechanisms are not well understood. Therefore, this study aims to reveal how high-fat diet causes skeletal muscle metabolic disorders. In total, 16 weaned rabbits were randomly divided into two groups, one group was fed a standard normal diet (SND) and the other group was fed a high fat diet (HFD) for 5 weeks. At the end of the five-week experiment, skeletal muscle tissue samples were taken from each rabbit. Untargeted metabolomic analysis was performed using ultra-performance liquid chromatography combined with mass spectrometry (UHPLC-MS/MS). The results showed that high fat diet significantly altered the expression levels of phospholipids, LCACs, histidine, carnosine, and tetrahydrocorticosterone in skeletal muscle. Principal component analysis (PCA) and least squares discriminant analysis (PLS-DA) showed that, compared with the SND group, skeletal muscle metabolism in HFD group was significantly up-regulated. Among 43 skeletal muscle metabolites in the HFD group, phospholipids, LCACs, histidine, carnosine, and tetrahydrocorticosteroids were identified as biomarkers of skeletal muscle metabolic diseases, and may become potential physiological targets of related diseases in the future. Untargeted metabonomics analysis showed that high-fat diet altered the metabolism of phospholipids, carnitine, amino acids and steroids in skeletal muscle of rabbits. Notably, phospholipids, LCACs, histidine, carnopeptide, and tetrahydrocorticosteroids block the oxidative capacity of mitochondria and disrupt the oxidative capacity of glucose and the fatty acid-glucose cycle in rabbit skeletal muscle.
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http://dx.doi.org/10.3390/ani11061722DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228676PMC
June 2021

Obesity Challenge Drives Distinct Maternal Immune Response Changes in Normal Pregnant and Abortion-Prone Mouse Models.

Front Immunol 2021 9;12:694077. Epub 2021 Jun 9.

Laboratory for Reproductive Immunology, NHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, China.

Obesity is prevalent among women of reproductive age and is associated with increased risk of developing multiple pregnancy disorders. Pregnancy must induce immune tolerance to avoid fetal rejection, while obesity can cause chronic inflammation through activating the immune system. Impaired maternal immuno-tolerance leads to pregnancy failure, such as recurrent spontaneous abortion (RSA), one of the most common complications during early pregnancy. How does maternal immune response change under obesity stress in normal pregnancy and RSA? In turn, is obesity affected by different gestational statuses? Limited information is presently available now. Our study investigated pregnancy outcomes and maternal immune responses in two murine models (normal pregnancy and spontaneous abortion models) after obesity challenge with a high-fat diet (HFD). Abortion-prone mice fed HFD had significantly higher weight gains during pregnancy than normal pregnant mice with HFD feeding. Nonetheless, the embryo implantation and resorption rates were comparable between HFD and normal chow diet (NCD)-fed mice in each model. Evaluation of immune cell subsets showed HFD-induced obesity drove the upregulation of activated NK cell-activating receptor (NKp46) NK cells and pro-inflammatory macrophages (MHCII M) as well as CD4 and CD8 T cells in the normal pregnancy group. However, in the abortion-prone group, relative more immature NK cells with decreased activity phenotypes were found in obese mice. Moreover, there were increased DCreg (CD11b DC) cells and decreased CD4 and CD8 T cells detected in the HFD abortion-prone mice relative to those fed the NCD diet. Our findings reveal how pregnancy obesity and maternal immune regulation are mutually influenced. It is worth noting that the abortion-prone model where active maternal immune status was intensified by obesity, in turn stimulated an overcompensation response, leading to an over-tolerized immune status, and predisposing to potential risks of perinatal complications.
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http://dx.doi.org/10.3389/fimmu.2021.694077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219966PMC
June 2021

Low-Cost, Environmentally Friendly, and High-Performance Triboelectric Nanogenerator Based on a Common Waste Material.

ACS Appl Mater Interfaces 2021 Jul 24;13(26):30776-30784. Epub 2021 Jun 24.

Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, P. R. China.

With the great progress in human activities and production technologies, the waste inevitably produced causes not only environmental pollution but also resource waste; meanwhile, the mobile and portable electronic devices urgently need a distributed and sustainable energy source to ensure their stable operation. Here, the waste pollutants (milk cartons) generated from daily life, commonly associated with environmental concerns, are instead identified as an available resource for preparing an emerging energy harvester (triboelectric nanogenerator, TENG), which can convert ubiquitous mechanical energy into electric power. Consequently, based on the waste material, the initial charge density of the TENG is as low as 0.035 mC m, which can be tremendously improved to 1.00 mC m through combining a charge excitation circuit, achieving efficient energy harvesting. In addition, compared to the common dielectric film, the waste material can reduce the cost and simplify the process of the preparation of TENG. This work provides not only an innovative approach to simultaneously realize environmental protection and energy harvesting but also more material choice for the preparation of a low-cost and high-performance TENG.
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http://dx.doi.org/10.1021/acsami.1c09192DOI Listing
July 2021

Comparison of human bone mineral densities in subjects on plant-based and omnivorous diets: a systematic review and meta-analysis.

Arch Osteoporos 2021 06 18;16(1):95. Epub 2021 Jun 18.

Department of Endocrinology, Hubei Province Jingmen Hospital of Traditional Chinese Medicine, No. 15, Baimiao Road, Duodao District, Jingmen City, 448000, Hubei Province, China.

Background: The increased consumption of plant-based diets has encouraged studies of bone mineral density (BMD). The present systematic review and meta-analysis compared the effects of plant-based and omnivorous diets on BMD.

Methods: We searched the Cochrane Library, PubMed, EMBASE, and the Web of Science to July 1, 2020. We used the mean differences (MDs) with 95% CIs to compare group outcomes. We compared the lumbar spine, femoral neck, and whole-body BMDs of those on plant-based and omnivorous diets. We performed subgroup analyses by various clinical characteristics. Two reviewers independently assessed trial quality and extracted data. All statistical analyses were performed using Review Manager version 5.2.

Results: A total of 17 cross-sectional studies including 13,888 patients were identified. The pooled results indicated that those on plant-based diets (compared to omnivores) exhibited lower BMDs at the lumbar spine (MD - 0.04; 95% CI - 0.06 to - 0.02; P < 0.0001) and femoral neck (MD - 0.04; 95% CI - 0.05 to - 0.02; P < 0.00001), and a reduced whole-body BMD (MD - 0.03; 95% CI - 0.06 to - 0.01; P = 0.0009). Both vegetarians and vegans exhibited lower lumbar spine, femoral neck, and whole-body BMDs than omnivores.

Conclusions: Plant-based diets were associated with lower BMDs than those of an omnivore population. Plant-based diets may compromise overall bone health; prospective research is required.
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http://dx.doi.org/10.1007/s11657-021-00955-0DOI Listing
June 2021

Cumulative Deficits Frailty Index Predicts Outcomes for Solid Organ Transplant Candidates.

Transplant Direct 2021 Mar 22;7(3):e677. Epub 2021 Feb 22.

Ajmera Transplant Centre, Toronto General Hospital, University Health Network, Toronto, Ontario, Canada.

Despite comprehensive multidisciplinary candidacy assessments to determine appropriateness for solid organ transplantation, limitations persist in identifying candidates at risk of adverse outcomes. Frailty measures may help inform candidacy evaluation. Our main objective was to create a solid organ transplant frailty index (FI), using the cumulative deficits model, from data routinely collected during candidacy assessments. Secondary objectives included creating a social vulnerability index (SVI) from assessment data and evaluating associations between the FI and assessment, waitlist, and posttransplant outcomes.

Methods: In this retrospective cohort study of solid organ transplant candidates from Toronto General Hospital, cumulative deficits FI and SVI were created from data collected during candidacy evaluations for consecutive kidney, heart, liver, and lung transplant candidates. Regression modeling measured associations between the FI and transplant listing, death or removal from the transplant waitlist, and survival after waitlist placement.

Results: For 794 patients, 40 variable FI and 10 variable SVI were created (258 lung, 222 kidney, 201 liver, and 113 heart transplant candidates). The FI correlated with assessment outcomes; patients with medical contraindications (mean FI 0.35 ± 0.10) had higher FI scores than those listed (0.29 ± 0.09), < 0.001. For listed patients, adjusted for age, sex, transplant type, and SVI, higher FI was associated with an increased risk of death (pretransplant or posttransplant) or delisting (hazard ratio 1.03 per 0.01 FI score, 95% confidence interval, 1.01-1.05, = 0.01).

Conclusions: A cumulative deficits FI can be derived from routine organ transplant candidacy evaluations and may identify candidates at higher risk of adverse outcomes.
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http://dx.doi.org/10.1097/TXD.0000000000001094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183975PMC
March 2021

Highly Selective CO Conversion to Methanol in a Bifunctional Zeolite Catalytic Membrane Reactor.

Angew Chem Int Ed Engl 2021 Jun 10. Epub 2021 Jun 10.

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, East China Normal University, 500 Dongchuan Road, 200241, Shanghai, China.

The hydrogenation of sequestrated CO to methanol can reduce CO emission and establish a sustainable carbon circuit. However, the transformation of CO into methanol is challenging because of the thermodynamic equilibrium limitation and the deactivation of catalysts by water. In the present work, different reactor types have been evaluated for CO catalytic hydrogenation to methanol. Best results have been obtained in a bifunctional catalytic membrane reactor (CMR) based on a zeolite LTA membrane and a catalytic Cu-ZnO-Al O -ZrO layer on top. Due to the in situ and rapid removal of the produced water from the catalytic layer through the hydrophilic zeolite LTA membrane, it is effective to break the thermodynamic equilibrium limitation, thus significantly increasing the CO conversion (36.1 %) and methanol selectivity (100 %). Further, the catalyst deactivation by the produced water can be effectively inhibited, thus maintaining a high long-term activity of the CMR.
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http://dx.doi.org/10.1002/anie.202106277DOI Listing
June 2021

In PICU acute kidney injury stage 3 or mortality is associated with early excretion of urinary renin.

Pediatr Res 2021 Jun 3. Epub 2021 Jun 3.

Department of Nephrology and Immunology, Children's Hospital of Soochow University, Suzhou, Jiangsu province, China.

Background: Urinary renin is proposed to be a novel prognostic biomarker of acute kidney injury (AKI) in adults. The intention of our study was to evaluate the early predictive value of urinary renin for AKI and pediatric intensive care unit (PICU) mortality in critically ill children.

Methods: The first available urine sample during the first 24 h after admission was collected upon PICU admission for the measurement of renin using ELISA. Urinary renin concentrations were corrected for urinary creatinine (urinary renin-to-creatinine ratio, uRenCR). AKI was defined based on KDIGO criteria.

Results: Of the 207 children, 22 developed AKI, including 6 with stage 1, 6 with stage 2, and 10 with stage 3, and 14 died during PICU stay. There was a significant difference in uRenCR between non-AKI children and those with AKI stage 3 (P = 0.001), but not with AKI stage 1 or 2. The uRenCR remained associated with AKI stage 3 and PICU mortality after adjustment for potential confounders. The area under the receiver operating characteristic curve of uRenCR for discrimination of AKI stage 3 was 0.805, and PICU mortality was 0.801.

Conclusions: Urinary renin was associated with the increased risk for AKI stage 3 and PICU mortality in critically ill children.

Impact: Urinary renin is proposed to be a novel prognostic biomarker of AKI in adult patients. There are some differences between children and adults in physiological and pathophysiological characteristics. This study demonstrated that urinary renin was associated with the increased risk for AKI stage 3 and PICU mortality in critically ill children. Accurate identification of patients with severe renal injury or at high risk for mortality early in the disease course could augment the efficacy of available interventions and improve patient outcomes.
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http://dx.doi.org/10.1038/s41390-021-01592-6DOI Listing
June 2021

Detection of sialic acid using boronic-acid-functionalized metal organic framework [email protected](OH).

Talanta 2021 Sep 18;232:122434. Epub 2021 Apr 18.

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, East China Normal University, Dongchuan Road 500, Shanghai, 200241, China. Electronic address:

Sialic acid (SA) is a crucial component of glycoproteins and glycolipids on the cellular membrane, which is essential for maintaining the function of cell membranes, such as cell recognition and communication. Simultaneously, sialic acid plays a significant role in many physiological and pathological processes. Hence, it is urgent to develop a simple and sensitive strategy for determining sialic acid. In this work, a new metal-organic framework called [email protected](OH) has been designed and synthesized for the recognition and detection of sialic acid. The boronic acid functional group in [email protected](OH) can bind to a diol moiety of the glycerol side chain of sialic acid, which will attenuate or even quench the fluorescence of [email protected](OH), thus opening a new road to detect sialic acid. Based on the measurement results, sialic acid can be quantitatively measured in a linear range of 0.05-2.5 mmoL/L with the [email protected](OH) probe. The detection limit of sialic acid is as low as 0.025 mmol/L. Furthermore, the boronic-acid functionalized probe [email protected](OH) displays high sensitivity and high selectivity to recognize the sialic acid in mouse serum samples. Therefore, the developed [email protected](OH) can be used as a promising probe to identify and detect sialic acid in the practical application.
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http://dx.doi.org/10.1016/j.talanta.2021.122434DOI Listing
September 2021

Untargeted Metabolomics Reveals Intestinal Pathogenesis and Self-Repair in Rabbits Fed an Antibiotic-Free Diet.

Animals (Basel) 2021 May 27;11(6). Epub 2021 May 27.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.

The prohibition of the use of growth-promoting drug additives in feeds was implemented in China in 2020. However, rabbits can experience symptoms of intestinal disease, such as diarrhea and flatulence, when switching from standard normal diets with antibiotics to antibiotic-free diets. The molecular mechanisms related to the occurrence of these diseases as well as associated physiological and metabolic changes in the intestine are unclear. Thus, the objectives of this study were to study the pathogenesis of intestinal inflammation using untargeted metabolomics. This was done to identify differential metabolites between a group of antibiotic-free feed Hyplus rabbits (Dia) whose diet was abruptly changed from a standard normal diet with antibiotics to an antibiotic-free diet, and an antibiotic diet group Hyplus rabbits (Con) that was fed a standard normal diet with antibiotics. Morphological damage to the three intestinal tissues was determined through visual microscopic examination of intestinal Dia and Con tissue samples stained with hematoxylin and eosin (HE). A total of 1969 different metabolites were identified in the three intestinal tissues from Dia and Con rabbits. The level of 1280 metabolites was significantly higher and the level of 761 metabolites was significantly lower in the Dia than in the Con group. These differential metabolites were involved in five metabolic pathways associated with intestinal inflammation (tryptophan metabolism, pyrimidine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, lysine degradation, and bile secretion). Rabbits in the Dia group developed metabolic disorders that affected the intestinal microbiota and changed the permeability of the intestinal tract, thereby triggering intestinal inflammation, affecting feed utilization, reducing production performance, and activating the intestinal tract self-repair mechanism. Thus, the abrupt transition from a diet with antibiotics to an antibiotic-free diet affected the structure and metabolism of the intestinal tract in Hyplus rabbits. Consequently, to avoid these problems, the antibiotic content in a rabbit diet should be changed gradually or alternative antibiotics should be found.
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http://dx.doi.org/10.3390/ani11061560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228699PMC
May 2021

Structural Characterization and Molecular Simulation of Baoqing Lignite.

ACS Omega 2021 Apr 6;6(15):10281-10287. Epub 2021 Apr 6.

Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

The molecular structure of Baoqing lignite was analyzed by ultimate analysis, Fourier transform infrared spectroscopy, X-ray diffraction spectroscopy, C solid-state nuclear magnetic resonance, and X-ray photoelectron spectroscopy. The results revealed that the aromaticity of Baoqing lignite is 27.64%, and the aromatic structure mainly contains benzene and naphthalene. The aliphatic structure consists of alkyl side chains and cycloalkyl. Oxygen atoms are present in phenol, ether, carbonyl, and carboxyl groups; nitrogen atoms are chiefly in pyridine and pyrrole; sulfur atoms mainly exist in sulfoxide sulfur. The molecular structure model of Baoqing lignite was constructed based on experimental data, and the molecular formula is CHONS. The molecular configuration was optimized by adopting the M06-2X basis set in the framework of density functional theory. Moreover, the simulated FTIR spectrum was in good agreement with the experimental spectra, proving the accuracy of the molecular structure. The molecular model of Baoqing lignite contains a majority of aliphatic structures and aromatic rings with a poor condensation degree. Moreover, the aromatic layers irregularly arrange in space.
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http://dx.doi.org/10.1021/acsomega.1c00627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153762PMC
April 2021

Key Markers and Epigenetic Modifications of Dental-Derived Mesenchymal Stromal Cells.

Stem Cells Int 2021 8;2021:5521715. Epub 2021 May 8.

Yunnan Provincial Key Laboratory of Stomatology, The Affiliated Stomatology Hospital of Kunming Medical University, Kunming, Yunnan, China.

As a novel research hotspot in tissue regeneration, dental-derived mesenchymal stromal cells (MSCs) are famous for their accessibility, multipotent differentiation ability, and high proliferation. However, cellular heterogeneity is a major obstacle to the clinical application of dental-derived MSCs. Here, we reviewed the heterogeneity of dental-derived MSCs firstly and then discussed the key markers and epigenetic modifications related to the proliferation, differentiation, immunomodulation, and aging of dental-derived MSCs. These messages help to control the composition and function of dental-derived MSCs and thus accelerate the translation of cell therapy into clinical practice.
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http://dx.doi.org/10.1155/2021/5521715DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128613PMC
May 2021

Ganoderma lucidum triterpenoids and polysaccharides attenuate atherosclerotic plaque in high-fat diet rabbits.

Nutr Metab Cardiovasc Dis 2021 06 6;31(6):1929-1938. Epub 2021 Apr 6.

Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, CAMS & PUMC, Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Beijing, 100021, China. Electronic address:

Background And Aims: Atherosclerosis is characterized by lipid deposition, oxidative stress, and inflammation in the arterial intima. Ganoderma lucidum triterpenoids (GLTs) and polysaccharides (GLPs) are traditional Chinese medicines with potential cardiovascular benefits. We aimed to comprehensively evaluate the effect of GLTs and GLPs on atherosclerosis and the associated underlying mechanisms in vivo and in vitro.

Methods And Results: Japanese big-ear white rabbits were randomly divided into three groups of blank, model, and treatment, and the treatment group was fed with GLSO and GLSP (0.3 g/kg body-weight/day) for 4 months. Serum levels of triglyceride (TG), total (TC), and low density lipoprotein cholesterol (LDL-C) in GL treatment group were significantly lower than those in the model group. The area of aortic plaques was significantly reduced in the treatment group. Further, GL administration in oxidized low-density lipoprotein (ox-LDL) stimulated human umbilical vein endothelial cells (HUVECs) reduced the generation of reactive oxygen species (ROS) and malondialdehyde (MDA) by inhibiting the upregulation of the nuclear transcription factor (NF)-κB p65 and the relative receptor LOX-1. In THP-1 cells treated with phorbol myristate acetate, GL inhibited the inflammatory polarization of macrophages (as evidenced by reduced TNF-α levels) via regulation of Notch1 and DLL4 pathways. Ox-LDL-stimulated THP-1 cells treated with GL showed an increase in the apoptosis of foam cells.

Conclusions: GLTs and GLPs attenuated the progression of atherosclerosis by alleviating endothelial dysfunction and inflammatory polarization of macrophages, thus promoting apoptosis of foam cells.
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http://dx.doi.org/10.1016/j.numecd.2021.03.023DOI Listing
June 2021

Multi-Omics Analysis of Key microRNA-mRNA Metabolic Regulatory Networks in Skeletal Muscle of Obese Rabbits.

Int J Mol Sci 2021 Apr 19;22(8). Epub 2021 Apr 19.

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

microRNAs (miRNAs), small non-coding RNA with a length of about 22 nucleotides, are involved in the energy metabolism of skeletal muscle cells. However, their molecular mechanism of metabolism in rabbit skeletal muscle is still unclear. In this study, 16 rabbits, 8 in the control group (CON-G) and 8 in the experimental group (HFD-G), were chosen to construct an obese model induced by a high-fat diet fed from 35 to 70 days of age. Subsequently, 54 differentially expressed miRNAs, 248 differentially expressed mRNAs, and 108 differentially expressed proteins related to the metabolism of skeletal muscle were detected and analyzed with three sequencing techniques (small RNA sequencing, transcriptome sequencing, and tandem mass tab (TMT) protein technology). It was found that 12 miRNAs and 12 core genes (e.g., , and ) were significantly different in skeletal muscle from rabbits in the two groups. The network analysis showed that seven miRNA-mRNA pairs were involved in metabolism. Importantly, two miRNAs (miR-92a-3p and miR-30a/c/d-5p) regulated three transcription factors (MYBL2, STAT1 and IKZF1) that may be essential for lipid metabolism. These results enhance our understanding of molecular mechanisms associated with rabbit skeletal muscle metabolism and provide a basis for future studies in the metabolic diseases of human obesity.
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http://dx.doi.org/10.3390/ijms22084204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072691PMC
April 2021

Integrated Proteomics and Metabolomics Analysis of Perirenal Adipose Tissue in Obese Rabbits Treated with a Restricted Diet.

Biology (Basel) 2021 Apr 12;10(4). Epub 2021 Apr 12.

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

In recent years, many people have shown an excess of fat accumulation. Known as obesity, this lesion poses an increased risk for multiple diseases, such as endocrine disease, diabetes, and cancer, and has reached epidemic proportions. Accompanied by the development of obesity, concern over body image and weight loss behavior is a growing social problem and public health threat, causing concern for many health professionals. However, the consequences of rapid weight loss remain largely unclear. Here, we applied an integrated proteomics and metabolomics analysis to investigate the effects of dieting on the proteins and metabolites in obese rabbits. Our study revealed that 343 differentially expressed proteins (136 upregulated and 207 downregulated) and 150 differentially expressed metabolites (91 upregulated and 59 downregulated) were identified. These molecules are mainly involved in the biological processes, including amino acid metabolism, lipid metabolism, and membrane and cytoskeleton reconstruction. The integrated analysis found that mevalonic acid, arachidonic acid, 15(S)-HpETE, cholecalciferol, hydrocortisone, lipoxin B4, lithocholic acid, etc. were associated with multiple pathways, and they may be the key factors to fight inflammation induced by a high-fat diet (HFD). Overall, this study provides further insight into the consequences of dieting-mediated weight loss and may contribute to the prevention and treatment of obesity.
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http://dx.doi.org/10.3390/biology10040321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069198PMC
April 2021

Phagocytosis of polymeric nanoparticles aided activation of macrophages to increase atherosclerotic plaques in ApoE mice.

J Nanobiotechnology 2021 Apr 28;19(1):121. Epub 2021 Apr 28.

Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400044, China.

The unique physiochemical properties of nanomaterials have been widely used in drug delivery systems and diagnostic contrast agents. The safety issues of biomaterials with exceptional biocompatibility and hemo-compatibility have also received extensive attention at the nanoscale, especially in cardiovascular disease. Therefore, we conducted a study of the effects of poly (lactic-co-glycolic acid) nanoparticles (PLGA NPs) on the development of aortic atherosclerotic plaques in ApoE mice. The particle size of PLGA NPs was 92.69 ± 3.1 nm and the zeta potential were - 31.6 ± 2.8 mV, with good blood compatibility. ApoE mice were continuously injected with PLGA NPs intravenously for 4 and 12 weeks. Examination of oil red O stained aortic sinuses confirmed that the accumulation of PLGA NPs caused a significantly higher extension of atherosclerotic plaques and increasing the expression of associated inflammatory factors, such as TNF-α and IL-6. The combined exposure of ox-LDL and PLGA NPs accelerated the conversion of macrophages to foam cells. Our results highlight further understanding the interaction between PLGA NPs and the atherosclerotic plaques, which we should consider in future nanomaterial design and pay more attention to the process of using nano-medicines on cardiovascular diseases.
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http://dx.doi.org/10.1186/s12951-021-00863-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082811PMC
April 2021

Genome-wide identification and characterization of perirenal adipose tissue microRNAs in rabbits fed a high-fat diet.

Biosci Rep 2021 Apr;41(4)

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

MicroRNAs (miRNAs) are a class of endogenous single-stranded RNA molecules that play an important role in gene regulation in animals by pairing with target gene mRNA. Extensive evidence shows that miRNAs are key players in metabolic regulation and the development of obesity. However, the systemic understanding of miRNAs in the adipogenesis of obese rabbits need further investigation. Here, seven small RNA libraries from rabbits fed either a standard normal diet (SND; n=3) or high-fat diet (HFD; n=4) were constructed and sequenced. Differentially expressed (DE) miRNAs were identified using the edgeR data analysis package from R. Software miRanda and RNAhybrid were used to predict the target genes of miRNAs. To further explore the functions of DE miRNAs, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed. A total of 81449996 clean reads were obtained from the seven libraries, of which, 52 known DE miRNAs (24 up-regulated, 28 down-regulated) and 31 novel DE miRNAs (14 up-regulated, 17 down-regulated) were identified. GO enrichment analysis revealed that the DE miRNAs target genes were involved in intermediate filament cytoskeleton organization, intermediate filament-based process, and α-tubulin binding. DE miRNAs were involved in p53 signaling, linoleic acid metabolism, and other adipogenesis-related KEGG pathways. Our study further elucidates the possible functions of DE miRNAs in rabbit adipogenesis, contributing to the understanding of rabbit obesity.
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http://dx.doi.org/10.1042/BSR20204297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082595PMC
April 2021

Vitamin D Levels and the Risk of Posttransplant Diabetes Mellitus After Kidney Transplantation.

Prog Transplant 2021 Jun 1;31(2):133-141. Epub 2021 Apr 1.

Division of Nephrology and the Kidney Transplant Program, 7989University Health Network, Toronto, Ontario, Canada.

Introduction: Given the burden of posttransplant diabetes mellitus and the high prevalence of low vitamin D levels in kidney transplant recipients, it is reasonable to consider vitamin D as a novel and potentially modifiable risk factor in this patient population.

Research Question: To determine the association between 25- hydroxyvitamin D (25(OH)D) level and posttransplant diabetes among kidney transplant recipients. Design: In a multi-center cohort study of 442 patients who received a kidney transplant between January 1, 2005 and December 31, 2010, serum samples within one-year before transplant were analyzed for 25(OH)D levels. The association between 25(OH)D and posttransplant diabetes were examined in Cox proportional hazard models.

Results: The median 25(OH)D level was 66 nmol/L. The cumulative probability of diabetes at 12-months by quartiles of 25(OH)D (< 42, 42 to 64.9, 65 to 94.9, and > 95 nmol/L) were 23.4%, 26.9%, 21.4%, and 15.6%, respectively. Compared to the highest 25(OH)D quartile, hazard ratios (95% CI) for the risk were 1.85 (1.03, 3.32), 2.01 (1.12, 3.60), 1.77 (0.96, 3.25) across the first to third quartiles, respectively. The associations were accentuated in a model restricted to patients on tacrolimus. When modeled as a continuous variable, 25(OH)D levels were significantly associated with a higher risk of diabetes (hazard ratio 1.06, 95% CI: 1.01, 1.13 per 10 nmol/L decrease).

Discussion: Serum 25(OH)D was an independent predictor of posttransplant diabetes in kidney transplant recipients. These results may inform the design of trials using vitamin D to reduce the risk in kidney transplant recipients.
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http://dx.doi.org/10.1177/15269248211002796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182337PMC
June 2021

Decidualization-derived cAMP regulates phenotypic and functional conversion of decidual NK cells from CD56CD16 NK cells.

Cell Mol Immunol 2021 Jun 30;18(6):1596-1598. Epub 2021 Mar 30.

NHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Hospital of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, China.

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http://dx.doi.org/10.1038/s41423-021-00675-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166858PMC
June 2021

What Are the Burden, Causes, and Costs of Early Hospital Readmissions After Kidney Transplantation?

Prog Transplant 2021 Jun 24;31(2):160-167. Epub 2021 Mar 24.

Division of Nephrology and the Kidney Transplant Program, Toronto General Hospital, University Health Network, University of Toronto, Toronto, Ontario, Canada.

Introduction: Kidney transplant recipients are at risk for complications resulting in early hospital readmission. This study sought to determine the incidences, risk factors, causes, and financial costs of early readmissions.

Design: This single-centre cohort study included 1461 kidney recipients from 1 Jul 2004 to 31 Dec 2012, with at least 1-year follow-up. Early readmission was defined as hospitalization within 30 or 90-days postdischarge from transplant admission. Associations between various parameters and 30 and 90-days posttransplant were determined using multivariable Cox proportional hazards models. The hospital-associated costs of were assessed.

Results: The rates of early readmission were 19.4% at 30 days and 26.8% at 90 days posttransplant. Mean cost per 30-day readmission was 11 606 CAD. Infectious complications were the most common reasons and resulted in the greatest cost burden. Factors associated with 30 and 90-days in multivariable models were recipient history of chronic lung disease (hazard ratio or HR 1.78 [95%CI: 1.14, 2.76] and HR 1.68 [1.14, 2.48], respectively), median time on dialysis (HR 1.07 [95% CI: 1.01, 1.13]and HR 1.06 [95% CI: 1.01, 1.11], respectively), being transplanted preemptively (HR 1.75 [95% CI: 1.07, 2.88] and HR 1.66 [95% CI: 1.07, 2.57], respectively), and having a transplant hospitalization lasting of and more than 11 days (HR 1.52 [95% CI: 1.01, 2.27] and HR 1.65 [95% CI: 1.16, 2.34], respectively).

Discussion: Early hospital readmission after transplantation was common and costly. Strategies to reduce the burden of early hospital readmissions are needed for all patients.
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http://dx.doi.org/10.1177/15269248211003563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182333PMC
June 2021

Ureteral strictures post-kidney transplantation: Trends, impact on patient outcomes, and clinical management.

Can Urol Assoc J 2021 Mar 18. Epub 2021 Mar 18.

Ajmera Transplant Centre, Toronto General Hospital, University Health Network, Toronto, ON, Canada.

Introduction: Ureteral strictures post-kidney transplantation (KT) can be a significant morbidity to the patient, often requiring surgical intervention and impacting graft function. We sought to investigate the incidence, clinical management, and outcomes of ureteral strictures among kidney transplant recipients (KTRs) at a large, multi-organ transplant center.

Methods: We conducted a single-center cohort study looking at KTRs who had transplant surgery from January 1, 2005 to March 31, 2017 with at least one-year followup (n=1742). Any KTRs done outside of our center or simultaneous multiorgan transplants were excluded. The Kaplan-Meier product-limit method was used to determine the incidence of ureteral strictures. Risk factors for ureteric strictures and clinical outcomes among patients with vs. without ureteric strictures were analyzed using Cox proportional hazards models.

Results: The incidence of ureteral strictures was 1.31 (95% confidence interval [CI] 0.85, 2.01) per 100 person-years or a cumulative incidence of 1.2%. We did not find any donor or recipient demographic variables that were independently associated with an increased risk of ureteral stricture development. A large proportion was managed successfully with radiologic intervention alone (47.6%). Ureteral strictures were associated with death-censored graft failure (hazard ratio [HR] 7.17, 95% CI 2.81, 18.30), total graft failure (HR 3.04, 95% CI 1.41, 6.59), and hospital readmission (HR 2.52, 95% CI 1.58, 4.00).

Conclusions: Although uncommon, ureteral strictures can significantly impact patient outcomes after KT. A better understanding of risk factors and clinical management will be important to ensure optimal graft outcomes.
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http://dx.doi.org/10.5489/cuaj.7003DOI Listing
March 2021

Novel Copper(II) Complex with a 4-Acylpyrazolone Derivative and Coligand Induce Apoptosis in Liver Cancer Cells.

ACS Med Chem Lett 2021 Mar 26;12(3):467-476. Epub 2021 Feb 26.

College of Life Sciences, Xinjiang Normal University, Urumqi 830054, China.

A novel pyrazolone-based copper complex [CuL(phen)(CHOH)][CuL(phen)]·CHCHOH·CHOH (P-FAH-Cu-phen) was synthesized and characterized. The asymmetric structural unit of P-FAH-Cu-phen was composed of two independent complex units [CuL(phen)(CHOH)] and [CuL(phen)]:Cu1 center with six coordination mode and Cu2 center with five coordination mode. The growth of BEL-7404 cells and H22 cells was significantly inhibited by P-FAH-Cu-phen with IC values of 1.175 μg/mL and 1.097 μg/mL, respectively, which were much lower than IC of cisplatin for BEL-7404 cells (23.32 μg/mL) and H22 cells (27.5 μg/mL). P-FAH-Cu-phen induced cell cycle arrest at G2/M and apoptosis in BEL-7404 cells through mitochondria- and endoplasmic reticulum stress-associated pathways. Moreover, P-FAH-Cu-phen significantly suppressed the migration of BEL-7404 cells and the tumor growth in H22 tumor mouse model without severe side effects and improved the survival of tumor mice. The results suggested that P-FAH-Cu-phen might be a potential drug candidate for the treatment of live cancer.
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http://dx.doi.org/10.1021/acsmedchemlett.0c00680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957940PMC
March 2021

Crosstalk Between Trophoblasts and Decidual Immune Cells: The Cornerstone of Maternal-Fetal Immunotolerance.

Front Immunol 2021 25;12:642392. Epub 2021 Feb 25.

Laboratory for Reproductive Immunology, NHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, China.

The success of pregnancy relies on the fine adjustment of the maternal immune system to tolerate the allogeneic fetus. Trophoblasts carrying paternal antigens are the only fetal-derived cells that come into direct contact with the maternal immune cells at the maternal-fetal interface. The crosstalk between trophoblasts and decidual immune cells (DICs) via cell-cell direct interaction and soluble factors such as chemokines and cytokines is a core event contributing to the unique immunotolerant microenvironment. Abnormal trophoblasts-DICs crosstalk can lead to dysregulated immune situations, which is well known to be a potential cause of a series of pregnancy complications including recurrent spontaneous abortion (RSA), which is the most common one. Immunotherapy has been applied to RSA. However, its development has been far less rapid or mature than that of cancer immunotherapy. Elucidating the mechanism of maternal-fetal immune tolerance, the theoretical basis for RSA immunotherapy, not only helps to understand the establishment and maintenance of normal pregnancy but also provides new therapeutic strategies and promotes the progress of immunotherapy against pregnancy-related diseases caused by disrupted immunotolerance. In this review, we focus on recent progress in the maternal-fetal immune tolerance mediated by trophoblasts-DICs crosstalk and clinical application of immunotherapy in RSA. Advancement in this area will further accelerate the basic research and clinical transformation of reproductive immunity and tumor immunity.
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http://dx.doi.org/10.3389/fimmu.2021.642392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947923PMC
February 2021