Publications by authors named "Yangyang Zhang"

227 Publications

RACK1 degrades MAVS to promote bovine ephemeral fever virus replication via upregulating E3 ubiquitin ligase STUB1.

Vet Microbiol 2021 Apr 28;257:109096. Epub 2021 Apr 28.

Ruminant Diseases Research Center, College of Life Sciences, Shandong Normal University, Jinan, 250014, China. Electronic address:

Receptors for activated C kinase 1 (RACK1) could competitively combine with mitochondrial antiviral signaling protein (MAVS) to inhibit the type I interferon (IFN) signaling pathway during viral infection in vitro. However, whether RACK1 can degrade MAVS to enhance viral replication is still unknown. In this study, we found that bovine epidemic fever virus (BEFV) infection triggered the expression of RACK1. Overexpression of RACK1 promoted BEFV replication, while knockdown of RACK1 inhibited the replication of BEFV. Further research showed that RACK1 inhibited the type I IFN signaling pathway during BEFV infection by degrading MAVS, and RACK1 degraded MAVS via the ubiquitin-proteasome system. Mechanistically, RACK1 up-regulated the expression of E3 ubiquitin ligase STIP1 homology and U-box containing protein 1 (STUB1), thereby promoting the ubiquitination and degradation of MAVS. In addition, RACK1 degraded MAVS by enhancing the interaction between STUB1 and MAVS but not via its interaction with STUB1. Overall, our study reveals a novel mechanism by which RACK1 inhibits the type I IFN signaling pathway to BEFV infection through degradation of MAVS, thereby promoting viral infection. These findings provide a new perspective for the MAVS degradation regulated by RACK1.
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http://dx.doi.org/10.1016/j.vetmic.2021.109096DOI Listing
April 2021

The Effects of Obesity on Mortality Following Coronary Artery Bypass Graft Surgery: A Retrospective Study from a Single Center in China.

Med Sci Monit 2021 Apr 27;27:e929912. Epub 2021 Apr 27.

Department of Cardiovascular Surgery, Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China (mainland).

BACKGROUND Coronary artery bypass graft (CABG) surgery has become a routine surgical procedure for patients with occlusive coronary artery atherosclerosis. Worldwide, increasing levels of obesity are associated with ischemic heart disease and systemic comorbidities. This retrospective study from a single center in China aimed to investigate the effects of obesity on patient mortality following CABG surgery. MATERIAL AND METHODS Patients undergoing CABG (N=1471) were grouped according to body mass index (BMI) as normal weight (N=596), overweight (N=684), or obese (N=191). Baseline clinical characteristics and outcomes were recorded. Logistic regression analysis was performed for 30-day postoperative mortality. Kaplan-Meier survival curves were plotted, and Cox regression analysis investigated risk and protective factors for long-term mortality, with subgroup analysis for differences between on-pump and off-pump CABG groups. RESULTS The 30-day postoperative mortality was 5.0% in the normal-weight group, 1.3% in the overweight group, and 0% in the obese group. BMI was an independent protective factor for 30-day postoperative mortality (odds ratio=0.748; 95% confidence interval, 0.640-0.874; P<0.001). The 10-year mortality for the groups was 13.2% (normal), 7.8% (overweight), and 12.7% (obese). The >20-year mortality rates for the groups were 33.0% (normal), 41.5% (overweight), and 12.7% (obese). There was no significant correlation between BMI and long-term mortality. Being overweight had a protective effect against long-term mortality in the off-pump CABG subgroup. CONCLUSIONS An "obesity paradox" was identified in postoperative outcomes in patients following CABG surgery, with an increased BMI associated with reduced 30-day postoperative mortality. This association was more significant in the off-pump CABG group.
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http://dx.doi.org/10.12659/MSM.929912DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088123PMC
April 2021

Effects of peak time of myocardial injury biomarkers on mid-term outcomes of patients undergoing OPCABG.

BMC Cardiovasc Disord 2021 Apr 24;21(1):208. Epub 2021 Apr 24.

Department of Cardiovascular Surgery, Shanghai East Hospital, School of Medicine,Tongji University, 150 Jimo Road, Shanghai, 200120, China.

Background: With the development of cardiac surgery techniques, myocardial injury is gradually reduced, but cannot be completely avoided. Myocardial injury biomarkers (MIBs) can quickly and specifically reflect the degree of myocardial injury. Due to various reasons, there is no consensus on the specific values of MIBs in evaluating postoperative prognosis. This retrospective study was aimed to investigate the impact of MIBs on the mid-term prognosis of patients undergoing off-pump coronary artery bypass grafting (OPCABG).

Methods: Totally 564 patients undergoing OPCABG with normal courses were included. Cardiac troponin T (cTnT) and creatine kinase myocardial band (CK-MB) were assessed within 48 h before operation and at 6, 12, 24, 48, 72, 96 and 120 h after operation. Patients were grouped by peak values and peak time courses of MIBs. The profile of MIBs and clinical variables as well as their correlations with mid-term prognosis were analyzed by univariable and multivariable Cox regression models.

Result: Continuous assessment showed that MIBs increased first (12 h after surgery) and then decreased. The peak cTnT and peak CK-MB occurred within 24 h after operation in 76.8% and 67.7% of the patients respectively. No significant correlation was found between CK-MB and mid-term mortality. Delayed cTnT peak (peak cTnT elevated after 24 h after operation) was correlated with lower creatinine clearance rate (69.36 ± 21.67 vs. 82.18 ± 25.17 ml/min/1.73 m), body mass index (24.35 ± 2.58 vs. 25.27 ± 3.26 kg/m), less arterial grafts (1.24 ± 0.77 vs. 1.45 ± 0.86), higher EuroSCORE II (2.22 ± 1.12 vs.1.72 ± 0.91) and mid-term mortality (26.5 vs.7.9%). Age (HR: 1.067, CI: 1.006-1.133), left ventricular ejection fraction (HR: 0.950, CI: 0.910-0.993), New York Heart Association score (HR: 1.839, CI: 1.159-2.917), total venous grafting (HR: 2.833, CI: 1.054-7.614) and cTnT peak occurrence within 24 h (HR: 0.362, CI: 0.196-0.668) were independent predictors of mid-term mortality.

Conclusion: cTnT is a better indicator than CK-MB. The peak value and peak occurrence of cTnT are related to mid-term mortality in patients undergoing OPCABG, and the peak phases have stronger predictive ability.

Trial Registration: Chinese Clinical Trial Registry, ChiCTR2000033850. Registered 14 June 2020, http://www.chictr.org.cn/edit.aspx?pid=55162&htm=4 .
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http://dx.doi.org/10.1186/s12872-021-02006-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066968PMC
April 2021

Exosomal LINC00355 derived from cancer-associated fibroblasts promotes bladder cancer cell proliferation and invasion by regulating miR-15a-5p/HMGA2 axis.

Acta Biochim Biophys Sin (Shanghai) 2021 Apr 21. Epub 2021 Apr 21.

Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.

We have previously demonstrated that exosomes derived from cancer-associated fibroblasts (CAFs) promote bladder cancer (BC) cell proliferation and invasion by transferring LINC00355. In this study, the molecular mechanisms underlying the pro-bladder cancer action of exosomal LINC00355 were explored. CAFs were obtained from BC tumor tissues, and normal fibroblasts (NFs) were obtained from adjacent normal tissues. Human BC cell lines (T24 and 5367) were incubated with NF-Exo (exosomes from NFs), CAF-Exo (exosomes from CAFs), CAFsi-Ctrl-Exo (exosomes from si-Ctrl-transfected CAFs), and CAFsi-LINC00355-Exo (exosomes from si-LINC00355-transfected CAFs). BC cell proliferation and invasion were evaluated by MTT and Transwell assays, respectively. The interaction between miR-15a-5p and LINC00355 or HMGA2 was examined by online bioinformatics analysis and luciferase activity assay. Results showed that HMGA2 is a direct target of miR-15a-5p, and LINC00355 functions as a sponge of miR-15a-5p to upregulate HMGA2 expression. The promoting effects of CAF-Exo on HMGA2 expression, cell proliferation, and cell invasion were hindered when LINC00355 expression was inhibited in BC cells. These promoting effects were also hindered when miR-15a-5p was overexpressed or HMGA2 was silenced in BC cells. In conclusion, exosomal LINC00355 derived from CAFs promotes BC cell proliferation and invasion by regulating miR-15a-5p/HMGA2 axis.
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http://dx.doi.org/10.1093/abbs/gmab041DOI Listing
April 2021

Evolution of secondary inorganic aerosols amidst improving PM air quality in the North China plain.

Environ Pollut 2021 Mar 30;281:117027. Epub 2021 Mar 30.

Department of Atmospheric Science, Colorado State University, Fort Collins, CO, 80523, USA.

The Clean Air Action implemented by the Chinese government in 2013 has greatly improved air quality in the North China Plain (NCP). In this work, we report changes in the chemical components of atmospheric fine particulate matter (PM) at four NCP sampling sites from 2012/2013 to 2017 to investigate the impacts and drivers of the Clean Air Action on aerosol chemistry, especially for secondary inorganic aerosols (SIA). During the observation period, the concentrations of PM and its chemical components (especially SIA, organic carbon (OC), and elemental carbon (EC)) and the frequency of polluted days (daily PM concentration ≥ 75 μg m) in the NCP, declined significantly at all four sites. Asynchronized reduction in SIA components (large decreases in SO with stable or even increased NO and NH) was observed in urban Beijing, revealing a shift of the primary form of SIA, which suggested the fractions of NO increased more rapidly than SO during PM pollution episodes, especially in 2016 and 2017. In addition, unexpected increases in the sulfur oxidation ratio (SOR) and the nitrogen oxidation ratio (NOR) were observed among sites and across years in the substantially decreased PM levels. They were largely determined by secondary aerosol precursors (i.e. decreased SO and NO), photochemical oxidants (e.g. increased O), temperature, and relative humidity via gas-phase and heterogeneous reactions. Our results not only highlight the effectiveness of the Action Plan for improving air quality in the NCP, but also suggest an increasing importance of SIA in determining PM concentration and composition.
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http://dx.doi.org/10.1016/j.envpol.2021.117027DOI Listing
March 2021

Analyzing the Effects of Hyperspectral ZhuHai-1 Band Combinations on LAI Estimation Based on the PROSAIL Model.

Sensors (Basel) 2021 Mar 7;21(5). Epub 2021 Mar 7.

School of Geography and Information Engineering, China University of Geosciences, Wuhan 430074, China.

Leaf area index (LAI) is a key biophysical variable to characterize vegetation canopy. Accurate and quantitative LAI estimation is significant for monitoring vegetation growth status. ZhuHai-1 (ZH-1), which is a commercial remote sensing micro-nano satellite, provides a possibility for quantitative detection of vegetation with high spatial and spectral resolution. However, the band characteristics of ZH-1 are closely related to the accuracy of vegetation monitoring. In this study, a simulation dataset containing 32 bands of ZH-1 was generated by using the PROSAIL model, which was used to analyze the performance of 32 bands for LAI estimation by using the hybrid inversion method. Meanwhile, the effect of different band combinations on LAI estimation was discussed based on sensitivity analysis and the correlation between bands. Then, the optimal band combination from ZH-1 hyperspectral satellite data for LAI estimation was obtained. LAI estimation was performed based on the selected optimal band combination of ZH-1 satellite images in Xiantao city, Hubei province, and compared with the Sentinel-2 normalized difference vegetation index (NDVI) values and LAI product. The results demonstrated that the obtained LAI map based on the optimal band combination of ZH-1 was generally consistent with the overall distribution of Sentinel-2 NDVI and the LAI product, but had a moderate correlation with Sentinel-2 LAI (R = 0.60), which may not favorably indicate the validity of indirect validation. However, the method of this study on the analysis of hyperspectral data bands has application potential to provide a reference for selecting appropriate bands of hyperspectral satellite data to estimate LAI and improve the application of hyperspectral data such as ZH-1 in vegetation monitoring.
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http://dx.doi.org/10.3390/s21051869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962187PMC
March 2021

Microbial mechanism of enhancing methane production from anaerobic digestion of food waste via phase separation and pH control.

J Environ Manage 2021 Jun 26;288:112460. Epub 2021 Mar 26.

Shenzhen Engineering Research Laboratory for Sludge and Food Waste Treatment and Resource Recovery, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China.

Phase separation and pH control are commonly used to improve methane production during anaerobic digestion (AD) of food waste, but their influencing mechanisms have not been fully discovered through microbial analysis. In this study, single-phase AD (SPAD), two-phase AD without pH control (TPAD-pHUC), and TPAD with fermentation pH controlled at 6.0 and 4.5 were conducted. The results showed that phase separation decreased the ratio of total bacteria to total archaea in the methanogenic phase. At the organic loading rate (OLR) of 1.9 g/(L·d), methanogenesis was dominated by acetoclastic Methanosaeta in both SPAD and TPAD-pHUC, while elevated Methanoculleus and active hydrogen production initiated a shift from the acetoclastic to hydrogenotrophic pathway in SPAD as OLR increased, eventually resulting in excessive acidification at OLR 3.2 g/(L·d). TPAD-pHUC was dominated by Methanosaeta with scarce hydrogen production genes, and thus maintained a delicate balance between fewer acidogens and methanogens at OLR 3.2-3.7 g/(L·d). TPAD with pH control exhibited higher methane yield (460-482 ml/g) at OLR 1.9 g/(L·d) due to the enhancement of protein degradation and the conversion from methylated compounds to methane by Methanosarcina. High Na concentration facilitated the proliferation of hydrogen production bacteria, but inhibited acetoclastic methanogenesis at OLR 2.4 g/(L·d). In comparison with SPAD and pH control, TPAD without pH control, integrating 4 d acidogenesis and 22 d methanogenesis, exhibited the best and steady performance at OLR 3.7 g/(L·d) with methane production exceeding 370 ml/g.
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http://dx.doi.org/10.1016/j.jenvman.2021.112460DOI Listing
June 2021

Deficiency of cold-inducible RNA-binding protein exacerbated monocrotaline-induced pulmonary artery hypertension through Caveolin1 and CAVIN1.

J Cell Mol Med 2021 May 23;25(10):4732-4743. Epub 2021 Mar 23.

Key Laboratory of Arrhythmias, Ministry of Education, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Cold-inducible RNA-binding protein (CIRP) was a crucial regulator in multiple diseases. However, its role in pulmonary artery hypertension (PAH) is still unknown. Here, we first established monocrotaline (MCT)-induced rat PAH model and discovered that CIRP was down-regulated predominantly in the endothelium of pulmonary artery after MCT injection. We then generated Cirp-knockout (Cirp-KO) rats, which manifested severer PAH with exacerbated endothelium damage in response to MCT. Subsequently, Caveolin1 (Cav1) and Cavin1 were identified as downstream targets of CIRP in MCT-induced PAH, and the decreased expression of these two genes aggravated the injury and apoptosis of pulmonary artery endothelium. Moreover, CIRP deficiency intensified monocrotaline pyrrole (MCTP)-induced rat pulmonary artery endothelial cells (rPAECs) injuries both in vivo and in vitro, which was counteracted by Cav1 or Cavin1 overexpression. In addition, CIRP regulated the proliferative effect of conditioned media from MCTP-treated rPAECs on rat pulmonary artery smooth muscle cells, which partially explained the exceedingly thickened pulmonary artery intimal media in Cirp-KO rats after MCT treatment. These results demonstrated that CIRP acts as a critical protective factor in MCT-induced rat PAH by directly regulating CAV1 and CAVIN1 expression, which may facilitate the development of new therapeutic targets for the intervention of PAH.
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http://dx.doi.org/10.1111/jcmm.16437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107102PMC
May 2021

Screening and bioinformatical analysis of differentially expressed genes in nasopharyngeal carcinoma.

J Cancer 2021 30;12(7):1867-1883. Epub 2021 Jan 30.

Department of Radiation Oncology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, China.

To identify differentially expressed genes via bioinformatical analysis for nasopharyngeal carcinoma (NPC) and explore potential biomarkers for NPC. We downloaded the NPC gene expression datasets (GSE40290, GSE53819) and obtained differentially expressed genes (DEGs) via GEO2R. Functional analysis of DEGs was performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. In order to explore the interaction of DEGs and screen the core genes, we established protein-protein interaction (PPI) network. Then the expression level, prognostic and diagnostic analysis of the core genes in NPC were performed to reveal their potential effects on NPC. Furthermore, we obtained the transcription factors (TF) and microRNAs of core genes to construct the coregulatory network. We obtained 124 up-regulated genes and 190 down-regulated genes in total. These genes were found to be related to signal transduction, extracellular matrix organization and cell adhesion based on GO analysis. KEGG analysis revealed that the NF-kappa B (NF-κB) signaling pathway, pathways in cancer were mainly enriched signaling pathways. 25 core genes were obtained by constructing PPI network. Then the high expression of 10 core genes in NPC were verified via GEPIA, Oncomine databases and laboratory experiments. The TF-microRNA coregulatory network of the 10 core genes was built. Survival and diagnostic analysis indicated that SPP1 had negative influence on the prognosis of NPC patients based on two datasets and nine up-regulated core genes (FN1, MMP1, MMP3, PLAU, PLAUR, SERPINE1, SPP1, COL8A1, COL10A1) might be diagnostic markers for NPC. Core genes of NPC were screened out by bioinformatical analysis in the present study and these genes may serve as prognostic and diagnostic biomarkers for NPC.
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http://dx.doi.org/10.7150/jca.48979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974527PMC
January 2021

Human Cytomegalovirus miR-US33as-5p Targets IFNAR1 to Achieve Immune Evasion During Both Lytic and Latent Infection.

Front Immunol 2021 5;12:628364. Epub 2021 Mar 5.

Institute of Health Service and Transfusion Medicine, Academy of Military Medical Sciences, Beijing, China.

As the first line of antiviral defense, type I interferon (IFN) binds IFN receptor 1 (IFNAR1) and IFNAR2 to activate the Jak-STAT signal transduction pathway, producing IFN-stimulated genes (ISGs) to control viral infection. The mechanisms by which human cytomegalovirus (HCMV) counteracts the IFN pathway are only partially defined. We show that miR-US33as-5p encoded by HCMV is expressed in both lytic and latent infection. By analysis with RNA hybrid and screening with luciferase reporter assays, we identified IFNAR1 as a target of hcmv-miR-US33as-5p, which was further verified by examining the expression of two IFNAR1 mutants and the binding of IFNAR1 to miR-US33as-5p/miR-US33as-5p-M1/miR-US33as-5p-M2. We found that after the transfection of miR-US33as-5p mimics into different cell lines, the phosphorylation of downstream proteins and ISG expression were downregulated. Immunofluorescence showed that the miR-US33as-5p mimics also inhibited STAT1 translocation into the nucleus. Furthermore, we constructed HCMV with mutant miR-US33as-5p and determined that the mutation did not affect HCMV replication. We found that MRC-5/human foreskin fibroblast (HFF) cells infected with ΔmiRNA HCMV exhibited higher IFNAR1 and ISG expression and a reduced viral load in the presence of exogenous IFN than cells infected with WT HCMV did, confirming that the knockout of miR-US33as-5p impaired viral resistance to IFN. Finally, we tested the effect of ΔmiRNA HCMV on THP-1 and d-THP-1 cells, common models of latent infection and reactivation, respectively. Again, we found that cells infected with ΔmiRNA HCMV showed a reduced viral load in the presence of IFN than the control cells did, confirming that miR-US33as-5p also affects IFN resistance during both latency and reactivation. These results indicate a new microRNA (miRNA)-based immune evasion mechanism employed by HCMV to achieve lifelong infection.
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http://dx.doi.org/10.3389/fimmu.2021.628364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973039PMC
March 2021

Exosomal LINC00355 derived from cancer-associated fibroblasts promotes bladder cancer cell resistance to cisplatin by regulating miR-34b-5p/ABCB1 axis.

Acta Biochim Biophys Sin (Shanghai) 2021 Apr;53(5):558-566

Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.

Cisplatin resistance is a major challenge for bladder cancer (BC). Evidence indicates that exosome derived from cancer-associated fibroblasts (CAF-Exo) can promote chemotherapy resistance in various human tumors by delivering bioactive molecules. We have previously demonstrated that CAF-derived exosomal LINC00355 promotes BC cell proliferation and invasion. However, the underlying mechanisms are still unclear. In this study, we aimed to investigate the role and mechanisms of CAF-derived exosomal LINC00355 in BC cell resistance to cisplatin. Exosomes were isolated from normal fibroblasts (NFs) and BC tumor-derived CAFs, namely, NF-Exo and CAF-Exo. CAFs were transfected with si-Ctrl or si-LINC00355 and then different exosomes were isolated, namely, CAFsi-Ctrl-Exo and CAFsi-LINC00355-Exo. The human BC cell lines (T24 and 5367) were incubated with NF-Exo, CAF-Exo, CAFsi-Ctrl-Exo, and CAFsi-LINC00355-Exo in the presence of cisplatin. MTT proliferation assay and flow cytometric analysis showed that CAF-Exo promoted BC cell resistance to cisplatin and upregulated ABCB1 expression in BC cells by transferring LINC00355 to BC cells. Luciferase activity assay confirmed the interaction between miR-34b-5p and LINC00355 or ABCB1. qRT-PCR and western blot analysis further showed that LINC00355 sponged miR-34b-5p to upregulate ABCB1 expression. However, the promoting effects of CAF-Exo on BC cell resistance to cisplatin were abolished by miR-34b-5p overexpression and ABCB1 silencing. In conclusion, exosomal LINC00355 derived from CAFs promotes BC cell resistance to cisplatin by regulating the miR-34b-5p/ABCB1 axis.
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http://dx.doi.org/10.1093/abbs/gmab023DOI Listing
April 2021

Distribution of perfluorooctane sulfonate in mice and its effect on liver lipidomic.

Talanta 2021 May 30;226:122150. Epub 2021 Jan 30.

Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing Mass Spectrum Center, Beijing, 100190, China; Graduate School, University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

Perfluorooctane sulfonate (PFOS) is an emerging persistent organic pollutant (POP), and the harm caused by the enrichment of PFOS in living organism has attracted more and more attention. In this work, animal exposure model to PFOS was established. Mass spectrometry (MS), mass spectrometry imaging (MSI), hematoxylin and eosin (H&E) staining and lipidomics were combined for the study of the organ targeting of PFOS, the toxicity and possible mechanism caused by PFOS. PFOS most accumulated in the liver, followed by the lungs, kidneys, spleen, heart and brain. Combined with H&E staining and matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) results, it was found that the accumulation of PFOS indeed caused damage in particular areas of specific organ, like in the liver and in the marginal area of the heart. This work found that PFOS could cross the blood-brain barrier, entered the brain and caused the neurotoxicity, which was surprising and might be the reason that high dose of PFOS could cause convulsions. From the liver lipidomic analysis, we found that PFOS exposure mainly affected glycerophospholipid metabolism and sphingolipid metabolism. The up-regulated ceramide and lysophosphatidylcholine (LPC) might lead to liver cell apoptosis, and the decrease in liver triglyceride (TG) content might result in insufficient energy in mice and cause liver morphological damage. Phosphatidylcholine (PC) synthesis via phosphatidylethanolamine N-methyltransferase (PEMT) pathway might be a mechanism of self-protection in animals against PFOS induced inflammation. This study might provide new insight into underlying toxicity mechanism after exposure to PFOS.
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http://dx.doi.org/10.1016/j.talanta.2021.122150DOI Listing
May 2021

Identification of Regulatory circRNAs Involved in the Pathogenesis of Acute Myocardial Infarction.

Front Genet 2020 3;11:626492. Epub 2021 Feb 3.

Shanghai East Hospital of Clinical Medical College, Nanjing Medical University, Shanghai, China.

Background: Acute myocardial infarction (AMI) has high morbidity and mortality worldwide. However, the pathogenesis of AMI is still unclear, and the impact of circular RNAs (circRNAs) on AMI has rarely been recognized and needs to be explored.

Materials And Methods: The circRNA array was applied to investigate the expression level of circRNAs in the blood samples of coronary arteries of three AMI patients and three normal persons. Principal component analysis (PCA) and unsupervised clustering analysis were performed to reveal the distinguished expression patterns of circRNAs. The miRNA expression profiles of AMI patients were identified from a public dataset from the Gene Expression Omnibus (GEO) database (GSE31568). The miRNA binding sites on the circRNAs were predicted by miRanda. The miRNA enrichment analysis and annotation tool were used to explore the pathways that the dysregulated circRNAs may participate in.

Results: In total, 142 differentially expressed circRNAs, including 89 upregulated and 53 downregulated in AMI samples, were identified by the differential expression analysis. AMI patients had quite different circRNA expression profiles to those of normal controls. Functional characterization revealed that circRNAs that had the potential to regulate miRNAs were mainly involved in seven pathways, such as the Runt-related transcription factor-1 (RUNX1) expression and activity-related pathway. Specifically, hsa_circRNA_001654, hsa_circRNA_091761, hsa_circRNA_405624, and hsa_circRNA_406698 were predicted to sponge four miRNAs including hsa-miR-491-3p, hsa-miR-646, hsa-miR-603, and hsa-miR-922, thereby regulating RUNX1 expression or activity.

Conclusion: We identified dysregulated blood circRNAs in the coronary arteries of AMI patients and predicted that four upregulated circRNAs were involved in the regulation of RUNX1 expression or activity through sponging four miRNAs.
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http://dx.doi.org/10.3389/fgene.2020.626492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886988PMC
February 2021

Prognostic Value of the Albumin-to-Alkaline Phosphatase Ratio before Chemoradiotherapy in Patients with Nonmetastatic Nasopharyngeal Carcinoma.

Chemotherapy 2021 Feb 18:1-7. Epub 2021 Feb 18.

The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

Introduction: The aim of this study was to evaluate the prognostic value of the albumin-to-alkaline phosphatase ratio (AAPR) in patients with nonmetastatic nasopharyngeal carcinoma (NPC).

Methods: Patients with nonmetastatic NPC who underwent chemoradiotherapy (CRT) were retrospectively analyzed. The AAPR was calculated using the last value of albumin to alkaline phosphatase that was measured within 1 week before CRT. The optimal cutoff value for the AAPR value was determined by an X-tile plot. Propensity score matching (PSM) was performed to balance the differences of the baseline characteristics. The Kaplan-Meier method and log-rank test were used to calculate the survival. A Cox proportional hazards regression model was conducted for the multivariate analysis.

Results: Totally, 87 patients with nonmetastatic NPC who underwent CRT were included in the analysis. The optimal cutoff level for the AAPR was 0.46. The group with an AAPR ≤0.46 was more likely to have poorer overall survival (OS), progression-free survival (PFS), and distant metastasis-free survival (DMFS) (p = 0.023, p = 0.031 and p = 0.027, for OS, PFS, and DMFS, respectively). In Cox proportional hazards analysis, high AAPR was a better prognostic predictor.

Conclusion: AAPR may be a reliable prognostic index for nonmetastatic NPC patients.
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http://dx.doi.org/10.1159/000513058DOI Listing
February 2021

Dental measurements based on a three-dimensional digital technique: A comparative study on reliability and validity.

Arch Oral Biol 2021 Apr 23;124:105059. Epub 2021 Jan 23.

Department of Prosthodontics, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xin Jiang Uygur Autonomous Region, China; Affiliated Stomatological Hospital of Xinjiang Medical University, Urumqi 830054, Xin Jiang Uygur Autonomous Region, China; Stomatology Research Institute of Xinjiang Uygur Autonomous Region. Urumqi 830054, Xin Jiang Uygur Autonomous Region, China. Electronic address:

Objective: In orthodontics and prosthodontics fields, it is often necessary to measure the crown dimensions of patient's teeth for further diagnosis and treatment. This study aims to assess the reliability and validity of dental measurements by comparing three-dimensional (3D) measurement of digital model derived from 3D handheld scanner with electronic digital caliper measurement of plaster model, providing a promising technology for dentistry.

Design: The mesiodistal diameter, buccolingual diameter and clinical crown height of 2800 teeth were measured on 100 sets of maxillary and mandibular dental plaster models as well as on the corresponding 3D digital models obtained by structured light scanning of the plaster models. Each measurement was performed twice by qualified operators and averaged. Twenty sets of maxillary and mandibular dentition models were randomly selected for intra and inter observer errors. The reliability was evaluated by intraclass correlation coefficient (ICC). Bland-Altman analysis was utilized to evaluate the validity of methods.

Results: Excellent reliability (ICC > 0.75) of intra and inter observer on traditional and digital methods were exhibited. Bland-Altman analysis showed the largest difference was the mesiodistal diameter (170/2800), and the smallest (130/2800) was the clinical crown height. Compared with other teeth, buccolingual diameter of incisors and molars, mesiodistal diameter and clinical crown height of premolars and molars displayed relative differences. The mean biases were close to zero, upper and lower 95 % limits of agreement were within 0.5 mm.

Conclusions: Linear measurements obtained from 3D technique are consistent with the conventional method. The 3D technology can be clinically accepted and suitable for dental metrology.
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http://dx.doi.org/10.1016/j.archoralbio.2021.105059DOI Listing
April 2021

Embedding ruthenium nanoparticles in the shell layer of titanium zirconium oxide hollow spheres to catalyze the degradation of alkali lignin under mild condition.

J Hazard Mater 2021 Jun 15;411:125161. Epub 2021 Jan 15.

Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.

To catalyze the degradation of lignin in refractory wastewater efficiently, a new nanocomposite with Ru nanoparticles embedded on the surface of TiZrO hollow spheres was fabricated with three method a "sol-gel + calcination + vacuum-impregnation" template method, and the unique binary composition of TiZrO/Ru prevented the aggregation of Ru and keep its high activity. During 3-h catalytic-oxidation at 160 °C and 2.0 MPa O, 98% alkali lignin was degraded and 70% organic carbon was mineralized with the catalysis of TiZrO/Ru, while the values were only 50% and 25% without analysts. The catalyst increased the catalytic-oxidation rate constant k (h) of alkali lignin from 0.282 h to 1.175 h because of high-efficiency hydroxyl radical production, as determined by EPR. LC-OCD showed that the catalyst decomposed alkali lignin with molecular weight 1-2 kDa to small molecules. Butyl acetate was the main intermediate product, which should be derived from the auto synthesis of butanol and acetic acid. In addition to high conversion efficiency, the catalyst had good stability with 95% capability after five cycles. In real biogas slurry treatment, an increase of biochemical to COD ratio from 0.28 to 0.51, with obvious decoloration, indicated TiZrO/Ru enhanced the biodegradability of the refractory wastewater significantly.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125161DOI Listing
June 2021

The complete chloroplast genome sequence of var (Bambusodae).

Mitochondrial DNA B Resour 2020 Oct 5;5(3):3467-3468. Epub 2020 Oct 5.

College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, P. R. China.

var is mainly distributed in South China to Southwest China, growing on hillsides or river banks. In the current study, we sequenced the complete chloroplast genome of var. and reported for the first time. The genome is 139,402 bp in total length, include a large single-copy (LSC) region of 82,936 bp, small single-copy (SSC) region of 12,868 bp, a pair of invert repeats (IR) regions of 21,799 bp. Plastid genome contains 132 genes, 85 protein-coding genes, 39 tRNA genes, and 8 rRNA genes. Phylogenetic analysis based on 25 chloroplast genomes indicates that var is closely related to , and in Bambusodae.
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http://dx.doi.org/10.1080/23802359.2020.1823275DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783032PMC
October 2020

Complete chloroplast genome sequence of (Bambusodae).

Mitochondrial DNA B Resour 2020 Jun 26;5(3):2609-2610. Epub 2020 Jun 26.

College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, PR China.

is found in Xinyi county, Maoming city, Guangdong province, China. In the current study, we sequenced the complete chloroplast genome of and reported for the first time. The genome was 139,444 bp in total length, including a large single-copy (LSC) region of 82,956 bp, a small single-copy (SSC) region of 12,897 bp, and a pair of invert repeats (IR) regions of 21,798 bp. Plastid genome comprised of 127 genes in total; 82 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Phylogenetic analysis based on 25 chloroplast genomes indicates that is closely related to in Bambusodae.
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http://dx.doi.org/10.1080/23802359.2020.1781577DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783132PMC
June 2020

Impact of the 2019 Novel Coronavirus Disease (COVID-19) Epidemic on Radiotherapy-Treated Patients with Cancer: A Single-Center Descriptive Study.

Cancer Manag Res 2021 7;13:37-43. Epub 2021 Jan 7.

Department of Radiation Oncology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui Province 230031, People's Republic of China.

Introduction: The present study investigated hospitalization data of patients receiving radiotherapy at Anhui Cancer Hospital during the COVID-19 epidemic and analyzed the impact of the epidemic on the clinical data of radiotherapy patients to provide references for the feasibility and safety of radiotherapy at other medical institutions.

Methods: The present study performed a retrospective analysis of hospitalization data of patients undergoing radiotherapy at the Radiation Department (from January 5 to March 19, 2020 according to the Chinese lunar calendar), who were defined as the epidemic group. Hospitalization data for patients undergoing radiotherapy during the same period in 2019 were used as the control group for comparison with the epidemic group in terms of sex, age, distribution of various cancer types, hospitalization costs, average length of stay, completion rate of radiotherapy, treatment mode, and purpose of radiotherapy.

Results: A total of 79 and 115 patients received radiotherapy in the epidemic group and control group, respectively. The number of patients who received radiotherapy declined 31.3% during the epidemic period. The number of head and neck cancer patients who received radiotherapy was 36 (45.57%) in the epidemic group and 32 (27.83%) in the control group, which was a significant difference ( =6.476, =0.011). The proportions of patients with other types of cancer decreased, with no significant difference between the two groups >0.05). No significant differences between the two groups were found in terms of other hospitalization data (>0.05).

Conclusion: The total number of patients who received radiotherapy decreased during the epidemic period, but the proportion of head and neck cancer increased. The epidemic had no significant effect on other hospitalization data. While strengthening prevention and control measures, we should actively perform radiotherapy to ensure that cancer patients receive timely and safe treatment.
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http://dx.doi.org/10.2147/CMAR.S281323DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7800447PMC
January 2021

Comparison of the predictive value of progesterone-related indicators for pregnancy outcomes of women undergoing the short-acting GnRH agonist long protocol: a retrospective study.

J Ovarian Res 2021 Jan 12;14(1):14. Epub 2021 Jan 12.

Department of Obstetrics & Gynecology, Peking University First Hospital, 100034, Beijing, China.

Background: There are many progesterone (P) elevation-related indicators for predicting pregnancy outcomes, including the serum P, P-to-oestradiol ratio (P/E), P-to-follicle index (PFI), and P-to-mature oocyte index (PMOI); however, due to inconsistencies in study populations and controlled ovarian hyperstimulation (COH) protocols among studies, these indicators are controversial. Moreover, no researchers have included these four commonly used indicators in one study to compare their predictive efficacies. The objective of this study was to compare the predictive value of P-related indicators for pregnancy outcomes of women undergoing the short-acting GnRH agonist long protocol.

Methods: A total of 612 infertile women undergoing IVF/ICSI were recruited for this study. Serum samples were obtained on the morning of HCG injection for serum P and E measurements. Transvaginal ultrasound was performed to determine the follicle count (≥ 14 mm in diameter). The number of mature oocytes was observed in the embryo laboratory after oocyte retrieval.

Results: In cases of P < 2.5 ng/ml, there was no significant difference in the serum P level or P/E between the pregnant group and the non-pregnant group. The PFI and PMOI of the pregnant group were significantly lower than those of the non-pregnant group. According to the stratified analysis of the ovarian response, only the PMI and PMOI of the pregnant women in the normal ovarian response group were lower than those of the non-pregnant women. To compare the predictive value of the PFI and PMOI in IVF/ICSI outcomes, the patients were divided into four groups. The good-quality embryo rate and clinical pregnancy rate were highest in Group A (low PFI and low PMOI) and lowest in Group D (high PFI and high PMOI). In the two groups with discordant PFI and PMOI, namely Group B (low PFI and high PMOI) and Group C (high PFI and low PMOI), the good-quality embryo rate and clinical pregnancy rate were not significantly different.

Conclusions: The PFI and PMOI had equal value in predicting clinical pregnancy outcomes in the normal ovarian response group undergoing the short-acting GnRH agonist long protocol. Each clinical centre can choose one of the indicators according to their actual situation in clinical practice and establish individual cut-off values for PFI and PMOI based on their own hormonal measurements.
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http://dx.doi.org/10.1186/s13048-021-00768-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802138PMC
January 2021

Gut microbiome analysis as a predictive marker for the gastric cancer patients.

Appl Microbiol Biotechnol 2021 Jan 6;105(2):803-814. Epub 2021 Jan 6.

Bengbu Medical College, Bengbu, Anhui, China.

Gut microbiota have been implicated in the development of cancer. Colorectal and gastric cancers, the major gastrointestinal tract cancers, are closely connected with the gut microbiome. Nevertheless, the characteristics of gut microbiota composition that correlate with gastric cancer are unclear. In this study, we investigated gut microbiota alterations during the progression of gastric cancer to identify the most relevant taxa associated with gastric cancer and evaluated the potential of the microbiome as an indicator for the diagnosis of gastric cancer. Compared with the healthy group, gut microbiota composition and diversity shifted in patients with gastric cancer. Different bacteria were used to design a random forest model, which provided an area under the curve value of 0.91. Verification samples achieved a true positive rate of 0.83 in gastric cancer. Principal component analysis showed that gastritis shares some microbiome characteristics of gastric cancer. Chemotherapy reduced the elevated bacteria levels in gastric cancer by more than half. More importantly, we found that the genera Lactobacillus and Megasphaera were associated with gastric cancer.Key Points• Gut microbiota has high sensitivity and specificity in distinguishing patients with gastric cancer from healthy individuals, indicating that gut microbiota is a potential noninvasive tool for the diagnosis of gastric cancer.• Gastritis shares some microbiota features with gastric cancer, and chemotherapy reduces the microbial abundance and diversity in gastric cancer patients.• Two bacterial taxa, namely, Lactobacillus and Megasphaera, are predictive markers for gastric cancer.
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http://dx.doi.org/10.1007/s00253-020-11043-7DOI Listing
January 2021

Successive biochar amendment affected crop yield by regulating soil nitrogen functional microbes in wheat-maize rotation farmland.

Environ Res 2021 03 30;194:110671. Epub 2020 Dec 30.

National Engineering Research Center for Wheat, State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, 450046, China.

Biochar has attracted increased attention because of its potential benefits for carbon sequestration, soil fertility, and contaminant immobilization. However, mechanism of long-term successive biochar amendment affected crop yield by regulating soil properties and nitrogen (N) functional microbes is still unclear by now. A field fixed experiment was carried out from 2011 to 2018 that aimed to study the effects of successive biochar on soil properties, soil nitrogen functional microbial genes, and grain yield in wheat and maize rotation farmland in Northern China. Four straw biochar treatments were tested in this study: 0 (BC0, CK), 2.25 (BC2.25), 6.75 (BC6.75), and 11.25 (BC11.25) Mg ha. The results showed that, after seven wheat-maize rotations, the total organic carbon (TOC), total N (TN), NO, available potassium (AK), and the C/N ratio in 0-20 cm topsoil were increased significantly following biochar application; however, there were no obvious differences in available phosphorus (AP) and NH among biochar treatments. Biochar also resulted in a significant increase in crop yield and NO accumulation in 0-200 cm soil layer, with the highest yield in BC6.75. Furthermore, a marked increase was found in the amoA gene abundance in topsoil; however, it decreased significantly with excessive biochar application (BC11.25). At wheat maturity, the nirS gene abundance consistently decreased following biochar application, whereas the nosZ gene abundance initially increased and then decreased (peaking in BC6.75); however, no obvious changes in the nirK gene were observed. At maize maturity, biochar significantly increased the nirS and nosZ gene abundance in topsoil, especially in BC6.75. In addition, redundancy analysis indicated that the soil moisture content, AP, AK, TN, TOC, NO, NH, pH, and C/N ratio had markedly effects on the abundance of the amoA, nirK, nirS, and nosZ genes. In general, biochar-induced alterations of soil properties resulted in changes of gene abundance of soil nitrifying and denitrifying bacteria, and eventually affecting crop yields.
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http://dx.doi.org/10.1016/j.envres.2020.110671DOI Listing
March 2021

Complete chloroplast genome sequence of (Arundinarodae).

Mitochondrial DNA B Resour 2020 1;5(3):2237-2238. Epub 2020 Jun 1.

College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, PR China.

is native to Guizhou Province, China, and grows at an altitude of 260-320 m. It can be used as a raw material for weaving and papermaking. In the current study, the complete chloroplast (cp) genome of was sequenced and is reported for the first time. The complete cp sequence was 139,504 bp, include large single-copy (LSC), small single-copy (SSC), and a pair of invert repeats (IR) region of 83,103 bp, 12,813 bp, and 21,793 bp, respectively. Besides, the plastid genome comprised a total of 132 genes, including protein-coding, tRNA, and rRNA genes as 85, 39, and 8 genes, respectively. Phylogenetic analysis based on 28 cp genomes reveals that is closely associated with in Arundinarodae.
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http://dx.doi.org/10.1080/23802359.2020.1768939DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510662PMC
June 2020

Improving characteristic band selection in leaf biochemical property estimation considering interrelations among biochemical parameters based on the PROSPECT-D model.

Opt Express 2021 Jan;29(1):400-414

At present, many studies have mainly focused on analyzing the sensitivity and correlation to select characteristic bands. However, the interrelations between biochemical parameters were ignored, which may significantly influence the accuracy of biochemical concentration retrieval. The study aims to propose a new band selection method and to focus on the improving magnitude of characteristic band combination in leaf trait estimation when taking interrelations among different traits into consideration. Thus, in this study, firstly a ranking- and searching-based method considering the sensitivity and correlation between different wavelengths, which can enhance the reliability of spectral band selection, was proposed to select a subset of characteristic bands for leaf structure index and five leaf biochemical parameters (including chlorophyll (Chl), carotenoid (Car), leaf dry matter per area (LMA), equivalent water thickness (EWT), and anthocyanin (Anth)) based on the PROSPECT-D model. These characteristic bands were then validated based on a physical model for retrieving five biochemical properties using one synthetic dataset and six experimental datasets on leaf-level spectra. Secondly, and more innovatively, to explore interrelations among different biochemical parameters, trait-trait band combinations were adopted to retrieve and analyze how the five biochemical participants above affected each other. The results demonstrated that the combination of LMA (809 and 2278 nm), EWT (1386, 1414, and 1894 nm) is more beneficial in LMA and EWT estimation than respective retrieval: LMA-EWT band combination retrieval improves R by 0.5782 and 0.1824 in two datasets, respectively, compared with solely LMA characteristic bands retrieval. What's more, the accuracy of Chl, EWT, Car, and Anth estimation can be also improved when considering interrelations between biochemical parameters. The experimental results show that the ranking- and searching-based method is an effective and efficient way to select a set of spectral bands related to the foliar information about plant traits, and trait-trait combinations, which focus on exploring latent interrelations between leaf traits, are useful in furthering improve retrieval accuracy. This research will provide notably advanced insight into identifying the spectral responses of biochemical traits in foliage and canopies.
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http://dx.doi.org/10.1364/OE.414050DOI Listing
January 2021

Cholesterol-Rich Lipid Rafts in the Cellular Membrane Play an Essential Role in Avian Reovirus Replication.

Front Microbiol 2020 2;11:597794. Epub 2020 Nov 2.

Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China.

Cholesterol is an essential component of lipid rafts in cellular plasma membranes. Although lipid rafts have been reported to have several functions in multiple stages of the life cycles of many different enveloped viruses, the mechanisms by which non-enveloped viruses, which lack outer lipid membranes, infect host cells remain unclear. In this study, to investigate the dependence of non-enveloped avian reovirus (ARV) infection on the integrity of cholesterol-rich membrane rafts, methyl-β-cyclodextrin (MβCD) was used to deplete cellular membrane cholesterol at the ARV attachment, entry, and post-entry stages. Treatment with MβCD significantly inhibited ARV replication at both the entry and post-entry stages in a dose-dependent manner, but MβCD had a statistically insignificant effect when it was added at the attachment stage. Moreover, MβCD treatment markedly reduced syncytium formation, which occurs at a relatively late stage of the ARV life cycle and is involved in cell-cell transmission and release. Furthermore, the addition of exogenous cholesterol reversed the effects mentioned above. Colocalization data also showed that the ARV proteins σC, μNS, and p10 prefer to localize to cholesterol-rich lipid raft regions during ARV infection. Altogether, these results suggest that cellular cholesterol in lipid rafts plays a critical role in ARV replication.
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http://dx.doi.org/10.3389/fmicb.2020.597794DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667042PMC
November 2020

Efficient and clean preparation of rare prosaikogenin D by enzymatic hydrolysis of saikosaponin B and response surface methodology optimization.

Enzyme Microb Technol 2020 Dec 13;142:109690. Epub 2020 Oct 13.

School of Pharmacy, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China. Electronic address:

Prosaikogenin D, a rare secondary saponin in Radix Bupleuri, has much higher in vivo bioactivities than its original glycoside saikosaponin B. Its preparation methods, such as conventional acid hydrolysis and column chromatograph, are unfriendly to environment with serious pollution and undesired products. The aim of this study was to establish an efficient and clean approach for convenient preparation of this rare steroid saponin based on the enzymatic hydrolysis. Cellulase was selected from four commercial enzymes due to its higher hydrolysis performance. Then the hydrolysis conditions were optimized by response surface methodology after preliminary investigation on affecting factors by single-factor experiments. The reaction system was constructed by 100 μg/mL of saikosaponin B and 8.00 mg/mL of cellulase, which was incubated in HAc-NaAc buffer (pH 4.7) at 60 °C for 33 h. Consequently, a high conversion ratio of the substrate has been achieved at 95.04 %. The newly developed strategy is an efficient and clean approach for the preparation of prosaikogenin D and it is a promising technology in industrial application.
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http://dx.doi.org/10.1016/j.enzmictec.2020.109690DOI Listing
December 2020

Intratumoral Glutathione Activatable Nanoprobes for Fluorescence and F Magnetic Resonance Turn-On Imaging.

Anal Chem 2020 12 11;92(23):15679-15684. Epub 2020 Nov 11.

State Key Laboratory of Chemical Resource Engineering, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, P. R. China.

Tumor microenvironment turn-on nanoprobes that could specifically detect the occurrence of diseases possess great potential in early diagnosis. Here, a GSH activated nanoprobe was designed for fluorescence and F magnetic resonance (MR) dual-modal turn-on imaging of tumors. Specifically, fluorescence AgInS quantum dots (QDs for fluorescence imaging) were co-encapsulated with perfluoro-15-crown-5-ether (PFCE forF MRI) by amphiphilic polymers and further coated with in situ formed manganese dioxide (MnO) nanoshells, which served as efficient fluorescence and F MR quenchers due to energy transfer and paramagnetic relaxation effects, respectively. The over-expressed GSH in tumors would decompose the MnO nanoshells, resulting in remarkable enhancement of both fluorescence and F MRI signals of the nanoprobes, accordingly lighting up the tumor site.
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http://dx.doi.org/10.1021/acs.analchem.0c04301DOI Listing
December 2020

Percutaneous endoscopic cervical foraminotomy as a new treatment for cervical radiculopathy: A systematic review and meta-analysis.

Medicine (Baltimore) 2020 Nov;99(45):e22744

Department of Spinal Surgery, the First Affiliated Hospital of University of South China, Hengyang, Hunan, People's Republic of China.

Background: Anterior cervical discectomy and fusion (ACDF) is the gold standard treatment for this cervical radiculopathy. Posterior endoscopic cervical foraminotomy (PECF), an effective alternative to ACDF, is becoming widely used by an increasing number of surgeons. However, comparisons of the clinical outcomes of ACDF and PECF remain poorly explored. The purpose of this study was to evaluate and compare visual analog scale (VAS)-arm scores, VAS-neck scores, neck disability index (NDI) scores, reoperation, and complications in PECF and ACDF.

Materials And Methods: We comprehensively searched electronic databases or platforms, including PubMed, Web of Science, EMBASE, and the Cochrane Controlled Trial Center, using the PRISMA guidelines. The required information, including VAS-arm scores, VAS-neck scores, NDI scores, reoperation, and complications, was extracted from qualified studies and independently tested and compared by 2 researchers. The methodological index for nonrandomized studies was used to evaluate study quality.

Results: Nine studies consisting of 230 males and 256 females were included. The mean age of the included patients was 49.6 years, and the mean follow-up time was 20.6 months. The VAS-arm scores were significantly higher, and VAS-neck scores and NDI scores of PECF showed greater improvement trends for PECF than ACDF. The complication proportion of patients with PECF was lower, while the proportion of reoperation was similar between PECF and ACDF. ACDF was the most common revision surgery. The most common complication of PECF was transient paresthesia.

Conclusion: Compared with ACDF, PECF is safe and effective in patients with unilateral cervical radiculopathy without myelopathy, and PECF does not increase the probability of reoperation and complications.
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http://dx.doi.org/10.1097/MD.0000000000022744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647593PMC
November 2020

Predictive ability of EuroSCORE II integrating cardiactroponin T in patients undergoing OPCABG.

BMC Cardiovasc Disord 2020 10 28;20(1):463. Epub 2020 Oct 28.

Department of Cardiovascular Surgery, Shanghai East Hospital, Tongji University School of Medicine, 150 Jimo Road, Shanghai, 200120, People's Republic of China.

Background: Preoperative risk evaluation systems are significant and important to the allocation of medical resources and the communication between doctors and patients. The European System for Cardiac Operative Risk Evaluation II (EuroSCORE II) is widely used in clinical practice. Cardiac troponin T (cTnT) can specifically and accurately reflect myocardial injury. Whether EuroSCORE II can improve the predictive power after integrating with cTnT is still unclear. This study was a retrospective single center study designed to assess the predictive ability of EuroSCORE II integrated with cTnT for patients undergoing isolated off-pump coronary artery bypass grafting (OPCABG).

Methods: This retrospective and observational cohort study included 1887 patients who underwent first isolated OPCABG. cTnT was detected within 48 h before operation in each patient. According to myocardial injury, patients were divided by cTnT into 4 stages. A new risk evaluation system was created through logistic regression with EuroSCORE II and myocardial injury classification as covariates. Then the two risk evaluation systems were comparatively assessed by regression analysis, receiver operator characteristic curves, net reclassification index, Bland-Altman plots and decision curve analysis.

Results: There were 43 in-hospital deaths, with a mortality of 2.30% (43/1887). The logistic regression analysis showed that preoperative myocardial injury classification was a significant risk factor for in-hospital mortality in both total cohort (OR 1.491, 95%CI 1.049-2.119) and subsets (OR 1.761, 95%CI 1.102-2.814). The new risk evaluation system has higher calibration and discrimination power than EuroSCORE II, both for overall cohort and subsets. Especially, the new system has obvious advantages in discrimination power in the subset of acute myocardial infarction (AUC 0.813 vs. 0.772, 0.906 vs. 0.841, and 0.715 vs. 0.646, respectively).

Conclusions: Both myocardial injury classification and EuroSCORE II are independent risk factors of in-hospital mortality in OPCABG patients. The new risk evaluation system has higher predictive ability than EuroSCORE II, especially in patients with a recent history of AMI.
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http://dx.doi.org/10.1186/s12872-020-01745-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7594339PMC
October 2020

Expression and promoter methylation status of OPCML and its functions in the inhibition of cell proliferation, migration, and invasion in breast cancer.

Breast Cancer 2021 Mar 27;28(2):448-458. Epub 2020 Oct 27.

Department of Oncology Surgery, General Hospital of Ningxia Medical University, Yinchuan, 750004, Ningxia, China.

Background: Opioid binding protein/cell adhesion molecule-like (OPCML) has been demonstrated to be a tumor suppressor gene, as it has been shown in previous studies to play a tumor-suppressive role in a variety of cancers. However, the role of OPCML in breast tumorigenesis remains unclear.

Methods: In this study, we analyzed OPCML expression in breast cancers and adjacent non-tumor tissue samples and examined its molecular function in the breast cancer-derived cell lines MDA-MB-231 and MCF7.

Results: We found that OPCML was downregulated in most breast cancer samples but that this protein was expressed in most adjacent non-tumor samples. The loss or downregulation of OPCML is associated with hypermethylation of its promoter. Methylation of the OPCML promoter was detected in all breast cancer cell lines and primary tumors but was not detected in surgical margin tissues and normal breast tissues. Furthermore, functional assays showed that ectopic OPCML expression could inhibit breast tumor cell proliferation in vivo and in vitro and further suppresses tumor cell migration and invasion.

Conclusion: Our results show that OPCML exerts its tumor-suppressive functions in human breast cancer cells. Moreover, the promoter-specific hypermethylation of OPCML plays an important role in human breast cancer development.
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http://dx.doi.org/10.1007/s12282-020-01179-9DOI Listing
March 2021