Publications by authors named "Yangyang Guo"

51 Publications

Itaconic acid exerts anti-inflammatory and antibacterial effects via promoting pentose phosphate pathway to produce ROS.

Sci Rep 2021 Sep 13;11(1):18173. Epub 2021 Sep 13.

Singapore Phenome Center, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, 636921, Singapore.

Itaconic acid is produced by immune responsive gene 1 (IRG1)-coded enzyme in activated macrophages and known to play an important role in metabolism and immunity. In this study, mechanism of itaconic acid functioning as an anti-inflammatory metabolite was investigated with molecular biology and immunology techniques, by employing IRG1-null (prepared with CRISPR) and wild-type macrophages. Experimental results showed that itaconic acid significantly promoted the pentose phosphate pathway (PPP), which subsequently led to significantly higher NADPH oxidase activity and more reactive oxygen species (ROS) production. ROS production increased the expression of anti-inflammatory gene A20, which in turn decreased the production of inflammatory cytokines IL-6, IL-1β and TNF-α. NF-κB, which can up-regulate A20, was also vital in controlling IRG1 and itaconic acid involved immune-modulatory responses in LPS-stimulated macrophage in this study. In addition, itaconic acid inhibited the growth of Salmonella typhimurium in cell through increasing ROS production from NADPH oxidase and the hatching of Schistosoma japonicum eggs in vitro. In short, this study revealed an alternative mechanism by which itaconic acid acts as an anti-inflammatory metabolite and confirmed the inhibition of bacterial pathogens with itaconic acid via ROS in cell. These findings provide the basic knowledge for future biological applications of itaconic acid in anti-inflammation and related pathogens control.
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http://dx.doi.org/10.1038/s41598-021-97352-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438069PMC
September 2021

Nanoantennas Involved Optical Plasmonic Cavity for Improved Luminescence of Quantum Dots Light-Emitting Diodes.

ACS Appl Mater Interfaces 2021 Sep 10. Epub 2021 Sep 10.

State Key Laboratory of Solidification Processing, Center for Nano Energy Materials, School of Materials Science and Engineering, Shaanxi Joint Laboratory of Graphene (NPU), Northwestern Polytechnical University, Xi'an 710072, P. R. China.

The optical plasmonic cavity (OPC) including the metallic optical nanoantennas and a metal film exhibits extreme field enhancement for the increased spontaneous emission rate of emitters. The resonance wavelength of the OPC can be easily controlled by the volume of the OPC and the localized surface plasmonic resonances (LSPRs) of the nanoantennas, facilitating the effective coupling of OPC and the emitters. However, involving the OPC into the light emission-enhanced solution-processed devices is still a difficult challenge. The trade-off between the metallic structure of OPC and the solution procedures limits the performance enhancement of the electrical-driven devices. In this work, we construct a device-compatible OPC that allows the characterization of the carrier dynamics of quantum dot (QD) films in the real devices in-suit. The radiative recombination rate and relaxation rate of carriers in QDs are increased by the LSPR effect of the silver nanocubes for luminescence enhancement. The OPC further increases the spontaneous emission rate of QD films, achieving a Purcell factor of 166 and improving the electroluminescence of the OPC-based QD light-emitting diodes (QLEDs). The design of the OPC-involved QLEDs offers a solution for addressing the limitation of fabrication of OPC-combined solution-processed optoelectronic light sources.
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http://dx.doi.org/10.1021/acsami.1c11995DOI Listing
September 2021

NUDCD1 knockdown inhibits the proliferation, migration, and invasion of pancreatic cancer via the EMT process.

Aging (Albany NY) 2021 07 29;13(14):18298-18309. Epub 2021 Jul 29.

School of Stomatology, Wenzhou Medical University, Wenzhou 110013, China.

NudC domain containing 1 (NUDCD1) is an oncoprotein frequently activated or upregulated in various human cancers, but its role in pancreatic cancer (PC) remains unknown. Thus, we aimed to determine the function and mechanism of NUDCD1 in PC. We employed Western blot and quantitative real-time polymerase chain reaction to assess NUDCD1 expression in cells and PC tissues. NUDCD1 was knocked down in Patu8988 and PANC-1 cells. We conducted real-time cell analysis, wound healing assay, transwell assay and colony formation assay to evaluate the metastatic and proliferative abilities of PC cells. Western blot was conducted to assess the expression of markers associated with apoptosis and epithelial-mesenchymal transition (EMT). Also, we established a tumor xenograft model to determine the role of NUDCD1 . NUDCD1 was overexpressed in PC tissues and cells. NUDCD1 knockdown suppressed the invasion, migration, and proliferative abilities of the cells and induced PC cell apoptosis. The specific mechanism of NUDCD1 was related to the modulation of the EMT process. Data obtained from experiments revealed that NUDCD1 knockdown inhibited the tumor growth, proliferation, and metastasis by modulating the EMT and inducing the apoptosis of PC cells.
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http://dx.doi.org/10.18632/aging.203276DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351729PMC
July 2021

Shenzhi Jiannao formula ameliorates vascular dementia in vivo and in vitro by inhibition glutamate neurotoxicity via promoting clathrin-mediated endocytosis.

Chin Med 2021 Jul 28;16(1):65. Epub 2021 Jul 28.

Shenzhen Hospital of Beijing University of Chinese Medicine (Longgang), No. 1 Dayun Road, Longgang District, Shenzhen, 518172, China.

Background: Synaptic damage and glutamate excitotoxicity have been implicated in the pathogenesis of vascular dementia (VD). Clathrin, RAB5B and N-methyl-D-aspartic acid receptor 1 (NMDAR1) proteins play a vital role in endocytosis of synaptic vesicles in neurons and glutamate over accumulation. Previous researches have been confirmed that Shenzhi Jiannao (SZJN) formula has an anti-apoptotic and neuroprotective effect in VD, but the underlying mechanisms are still unclear. In this study, we aimed to explore the effect of SZJN formula on cognitive impairment and glutamate excitotoxicity via clathrin-mediated endocytosis (CME) in vivo and in vitro.

Methods: SZJN formula consists of Panax ginseng C.A.Mey., Anemarrhena asphodeloides Bunge, and Paeonia anomala subsp. veitchii (Lynch) D.Y.Hong & K.Y.Pan. All herbs were prepared into granules. Both common carotid arteries were permanent occluded (2-vessel occlusion, 2VO) in male Sprague Dawley (SD) rats to model VD. One day after operation, the rats began daily treatment with SZJN formula for 2 weeks. The neuroprotective effects of SZJN formula was subsequently assessed by the novel object recognition test, Morris water maze, hematoxylin-eosin (HE) staining and Nissl staining. Glutamate cytotoxicity was assessed by detecting cell viability and cell death of PC12 cells. Immunohistochemistry, immunofluorescence, Western blot, and quantitative real-time PCR were used to detect the expression levels of clathrin, RAB5B, and NMDAR1.

Results: Administration of SZJN formula effectively improved short-term memory and spatial memory. SZJN formula treatment significantly reduced hippocampal neuronal loss, and recovered the arrangement and morphology of neurons and Nissl bodies. Moreover, SZJN formula promoted the proliferation of PC12 cells and inhibited glutamate-induced cell death. The down-regulation of clathrin and RAB5B, as well as the upregulation of NMDAR1 in the brain induced by 2VO or glutamate was also notably reversed by SZJN formula at both the protein and mRNA levels, which may contribute to SZJN formula induced improved neurological function.

Conclusions: Taken together, our findings provide evidence that the neuroprotective effects of SZJN formula in experimental VD maybe mediated through promoting the expression of clathrin-mediated endocytosis and reducing NMDARs-associated glutamate excitotoxicity. SZJN formula serves as a promising alternative therapy and may be a useful herbal medicine for preventing progression of VD.
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http://dx.doi.org/10.1186/s13020-021-00477-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317332PMC
July 2021

The anti-dysenteric drug fraxetin enhances anti-tumor efficacy of gemcitabine and suppresses pancreatic cancer development by antagonizing STAT3 activation.

Aging (Albany NY) 2021 07 28;13(14):18545-18563. Epub 2021 Jul 28.

Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.

Fraxetin, a natural product isolated and purified from the bark of , has anti-inflammatory, analgesic, and anti-dysenteric activities. This study aimed to investigate the anti-tumor effects of fraxetin in pancreatic ductal adenocarcinoma (PDA). The effects of fraxetin on the malignant biological behavior of PDA were evaluated. Besides, the effects of fraxetin on the sensitivity of PCCs to gemcitabine, angiogenesis, the epithelial-mesenchymal transition (EMT), glucose metabolism, reactive oxygen species (ROS), and STAT3 activity were analyzed. By reversing the EMT, fraxetin suppressed proliferation, invasion, and migration, and induced mitochondrial-dependent apoptosis in PCCs. Also, treatment with fraxetin inhibited PDA growth and metastasis in nude mouse models. Furthermore, fraxetin made PCCs more sensitive to the chemotherapy drug gemcitabine. Mechanically, fraxetin treatment suppressed oncogenic KRAS-triggered STAT3 activation in PCCs and PDA tissues. Fraxetin shows significant interactions with STAT3 Src Homology 2 (SH2) domain residues, thereby preventing its homo-dimer formation, which then blocks the activation of downstream signal pathways. The anti-tumor activity of fraxetin in PDA was functionally rescued by a STAT3 activator colivelin. As a result, fraxetin hindered hypoxia-induced angiogenesis by decreasing HIF-1α and VEGFA expression, controlled glucose metabolism by reducing GLUT1 expression, inhibited the EMT by blocking the Slug-E-cadherin axis, and drove ROS-mediated apoptosis by regulating the STAT3-Ref1 axis. In conclusion, fraxetin enhances the anti-tumor activity of gemcitabine and suppresses pancreatic cancer development by antagonizing STAT3 activation.
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http://dx.doi.org/10.18632/aging.203301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351699PMC
July 2021

Low-medium temperature application of selective catalytic reduction denitration in cement flue gas through a pilot plant.

Chemosphere 2021 Aug 3;276:130182. Epub 2021 Mar 3.

Key Laboratory of Green Process and Engineering, National Engineering Laboratory for Hydrometallurgical Cleaner Production Technology, Institute of Process Engineering, Innovation Academy for Green Manufacture, Chinese Academy of Sciences, Beijing, 100190, China; Center for Excellence in Regional Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China. Electronic address:

Low-medium temperature application of selective catalytic reduction (SCR) denitration in cement flue gas was established and investigated in this study. The 2000 h continuous operation shows the concentration of NO at the outlet can be maintained at 24 mg/Nm on average, while due to the increase of SO in flue gas, the NO concentration increased to 57.5 mg/Nm after long time operation. The sulfur deposition is the main reason for catalyst deactivation, and SO is still a big obstacle for low-medium temperature SCR application in cement flue gas. The denitration efficiency was tested as fluctuated from 73.5% to 86.2%, and ammonia concentration after SCR was as still as high as 22.5-60.0 mg/Nm due to the excessive ammonia injection from selective non-catalytic reduction (SNCR), shows serious ammonia escape problem for SNCR, and the potential application of hybrid SNCR-SCR technology. In order to maintain the denitration efficiency above 85.0%, the gaseous hourly space velocity (GHSV) should not be exceeded 2800 h, the electrostatic precipitators (ESP) setting at 60 kV was relatively appropriate, the temperature of the flue gas should be kept at above 200 °C. The concentrations and toxic equivalent quantities (TEQs) of the PCDD/Fs congeners in the flue gas raised greatly after SCR reactor, indicating the PCDD/Fs concentration should be concerned during the application of low-medium temperature SCR, especially for the waste co-disposal processes.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130182DOI Listing
August 2021

Correction to: Chemopreventive effect of Betulinic acid via mTOR -Caspases/Bcl2/Bax apoptotic signaling in pancreatic cancer.

BMC Complement Med Ther 2021 Apr 26;21(1):131. Epub 2021 Apr 26.

Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, Wenzhou Medical University First Affiliated Hospital, Wenzhou, Zhejiang, China.

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http://dx.doi.org/10.1186/s12906-021-03254-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074483PMC
April 2021

Impaired Intestinal Akkermansia muciniphila and Aryl Hydrocarbon Receptor Ligands Contribute to Nonalcoholic Fatty Liver Disease in Mice.

mSystems 2021 Feb 23;6(1). Epub 2021 Feb 23.

Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Innovation Academy for Precision Measurement Science and Technology, CAS, Wuhan, China

Noncaloric artificial sweeteners (NAS) are extensively introduced into commonly consumed drinks and foods worldwide. However, data on the health effects of NAS consumption remain elusive. Saccharin and sucralose have been shown to pass through the human gastrointestinal tract without undergoing absorption and metabolism and directly encounter the gut microbiota community. Here, we aimed to identify a novel mechanism linking intestinal and the aryl hydrocarbon receptor (AHR) to saccharin/sucralose-induced nonalcoholic fatty liver disease (NAFLD) in mice. Saccharin/sucralose consumption altered the gut microbial community structure, with significant depletion of abundance in the cecal contents of mice, resulting in disruption of intestinal permeability and a high level of serum lipopolysaccharide, which likely contributed to systemic inflammation and caused NAFLD in mice. Saccharin/sucralose also markedly decreased microbiota-derived AHR ligands and colonic AHR expression, which are closely associated with many metabolic syndromes. Metformin or fructo-oligosaccharide supplementation significantly restored and AHR ligands in sucralose-consuming mice, consequently ameliorating NAFLD. Our findings indicate that the gut-liver signaling axis contributes to saccharin/sucralose consumption-induced NAFLD. Supplementation with metformin or fructo-oligosaccharide is a potential therapeutic strategy for NAFLD treatment. In addition, we also developed a new nutritional strategy by using a natural sweetener (neohesperidin dihydrochalcone [NHDC]) as a substitute for NAS and free sugars.
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http://dx.doi.org/10.1128/mSystems.00985-20DOI Listing
February 2021

A Machine Vision-Based Method Optimized for Restoring Broiler Chicken Images Occluded by Feeding and Drinking Equipment.

Animals (Basel) 2021 Jan 8;11(1). Epub 2021 Jan 8.

Department of Poultry Science, University of Georgia, Athens, GA 30602, USA.

The presence equipment (e.g., water pipes, feed buckets, and other presence equipment, etc.) in the poultry house can occlude the areas of broiler chickens taken via top view. This can affect the analysis of chicken behaviors through a vision-based machine learning imaging method. In our previous study, we developed a machine vision-based method for monitoring the broiler chicken floor distribution, and here we processed and restored the areas of broiler chickens which were occluded by presence equipment. To verify the performance of the developed restoration method, a top-view video of broiler chickens was recorded in two research broiler houses (240 birds equally raised in 12 pens per house). First, a target detection algorithm was used to initially detect the target areas in each image, and then Hough transform and color features were used to remove the occlusion equipment in the detection result further. In poultry images, the broiler chicken occluded by equipment has either two areas (TA) or one area (OA). To reconstruct the occluded area of broiler chickens, the linear restoration method and the elliptical fitting restoration method were developed and tested. Three evaluation indices of the overlap rate (OR), false-positive rate (FPR), and false-negative rate (FNR) were used to evaluate the restoration method. From images collected on d2, d9, d16, and d23, about 100-sample images were selected for testing the proposed method. And then, around 80 high-quality broiler areas detected were further evaluated for occlusion restoration. According to the results, the average value of OR, FPR, and FNR for TA was 0.8150, 0.0032, and 0.1850, respectively. For OA, the average values of OR, FPR, and FNR were 0.8788, 0.2227, and 0.1212, respectively. The study provides a new method for restoring occluded chicken areas that can hamper the success of vision-based machine predictions.
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http://dx.doi.org/10.3390/ani11010123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826920PMC
January 2021

Short-Term Intake of Hesperetin-7--Glucoside Affects Fecal Microbiota and Host Metabolic Homeostasis in Mice.

J Agric Food Chem 2021 Feb 22;69(5):1478-1486. Epub 2020 Dec 22.

Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy of Precision Measurement Science and Technology, CAS, Wuhan 430071, China.

Hesperetin-7--glucoside (Hes-7-G) is a typical flavonoid monoglucoside isolated from Citri Reticulatae Pericarpium (CRP), which is commonly used as a food adjuvant and exhibits potential biological activities. To explore the interaction between Hes-7-G ingestion and microbiome and host metabolism, here, 16S rRNA gene sequencing was first used to analyze the alteration of fecal microbiome in mice after Hes-7-G intake. Metabolic homeostasis in mice was subsequently investigated using untargeted H NMR-based metabolomics and targeted metabolite profiling. We found that dietary Hes-7-G significantly regulated fecal microbiota and its derived metabolites, including short-chain fatty acids (SCFAs) and tryptophan metabolites (indole and its derivatives), in feces of mice. Regulation of microbiota was further confirmed by the significantly changed urinary hippurate and trimethylamine -oxide (TMAO), co-metabolites of the microbe and host. We also found that dietary Hes-7-G modulated the host tricarboxylic acid cycle (TCA) involved in energy metabolism. These findings suggested that Hes-7-G exhibits potential beneficial effects for human health.
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http://dx.doi.org/10.1021/acs.jafc.0c05921DOI Listing
February 2021

CC-223, NSC781406, and BGT226 Exerts a Cytotoxic Effect Against Pancreatic Cancer Cells via mTOR Signaling.

Front Pharmacol 2020 11;11:580407. Epub 2020 Nov 11.

Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, Wenzhou Medical University First Affiliated Hospital, Wenzhou, China.

The mTOR signaling pathway is abnormally activated in pancreatic cancer and is related to tumor glucose metabolism. However, its specific regulation mechanism is still unclear. Therefore, this study aims to investigate whether Sestrin2 affects the glucose metabolism of pancreatic cancer by modulating mTOR signal and then affects its biological behavior. We have observed that l-leucine can promote the proliferation of pancreatic cancer cells and increase the expression of Sestrin2 and p-mTOR proteins. In order to further study the role of Sestrin2 and mTOR signaling in pancreatic cancer, we conducted Sestrin2 overexpression and mTOR pharmacological inhibition experiments. We found that Sestrin2 overexpression can increase glycolysis of pancreatic cancer cells and promote their proliferation. This effect can be eliminated by mTOR inhibitors. Finally, we found that Sestrin2 knockdown could inhibit the growth of pancreatic cancer . In conclusion, these findings suggest that Sestrin2 may promote the occurrence and development of pancreatic cancer through mTOR signaling.
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http://dx.doi.org/10.3389/fphar.2020.580407DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7741184PMC
November 2020

Solution-processed PEDOT:PSS:GO/Ag NWs composite electrode for flexible organic light-emitting diodes.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Mar 1;248:119267. Epub 2020 Dec 1.

School of Physical Science and Information Technology, Liaocheng University, Shandong 252059, China; Shandong Provincial Key Laboratory of Optical Communication Science and Technology, Shandong 252059, China; Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), School of Physical and Mathematical Sciences, Nanjing Tech University (NanjingTech), Nanjing 211800, China.

Flexible organic light emitting diodes (OLEDs) have attracted considerable attention for the reason of light weight, high mechanical flexibility in display and lighting. The most widely used transparent anode indium tin oxide (ITO) is unsuitable for flexible OLEDs because of its easy cracking upon bending. In this paper, we proposed a simple two steps solution processing method to fabricate flexible PEDOT:PSS:GO/Ag NWs composite electrodes. The optimized PEDOT:PSS:GO/Ag NWs composite electrode exhibits an optical transmittance of 88.7% at a wavelength of 550 nm and a low sheet resistance of 17 Ω/sq, which arecomparable to that of ITO. With PEDOT:PSS:GO/Ag NWs composite electrodes, the turn on voltage, current density and maximum brightness of OLEDs based on composite electrode were 2.1 V, 6.2 cd/A and 22894 cd/m, respectively, which were superior to that OLED based on ITO anode. The enhanced performance of OLEDs based on composite anode mainly attributed to the lower sheet resistance, smoother surface of the composite anode and the far surface plasma resonance (Far SPR) effect, a lower waveguide optical loss because of the introduction of Ag NWs in the electrode.
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http://dx.doi.org/10.1016/j.saa.2020.119267DOI Listing
March 2021

Effects of long-term antibiotic treatment on mice urinary aromatic amino acid profiles.

Biosci Rep 2021 01;41(1)

Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

The gut microbiota-host co-metabolites are good indicators for representing the cross-talk between host and gut microbiota in a bi-direct manner. There is increasing evidence that levels of aromatic amino acids (AAAs) are associated with the alteration of intestinal microbial community though the effects of long-term microbial disturbance remain unclear. Here we monitored the gut microbiota composition and host-microbiota co-metabolites AAA profiles of mice after gentamicin and ceftriaxone treatments for nearly 4 months since their weaning to reveal the relationship between host and microbiome in long- term microbial disturbances. The study was performed employing targeted LC-MS measurement of AAA-related metabolites and 16S RNA sequence of mice cecal contents. The results showed obvious decreased gut microbial diversity and decreased Firmicutes/Bacteroidetes ratio in the cecal contents after long-term antibiotics treatment. The accumulated AAA (tyrosine, phenylalanine and tryptophan) and re-distribution of their downstreaming metabolites that produced under the existence of intestinal flora were found in mice treated with antibiotics for 4 months. Our results suggested that the long-term antibiotic treatment significantly changed the composition of the gut microbiota and destroyed the homeostasis in the intestinal metabolism. And the urinary AAA could be an indicator for exploring interactions between host and gut microbiota.
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http://dx.doi.org/10.1042/BSR20203498DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786327PMC
January 2021

Inhibition of proliferation-linked signaling cascades with atractylenolide I reduces myofibroblastic phenotype and renal fibrosis.

Biochem Pharmacol 2021 01 19;183:114344. Epub 2020 Nov 19.

Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China; Institute of Chronic Kidney Disease, Wenzhou Medical University, Wenzhou 325000, China; Center for Health Assessment, Wenzhou Medical University, Wenzhou 325000, China. Electronic address:

Renal fibrosis is a frequent axis contributing to the occurrence of end-stage nephropathy. Previously, it has been reported that atractylenolide Ⅰ (ATL-1), a natural compound extracted from Atractylodes macrocephala, has anti-cancer and antioxidant effects. However, the renal anti-fibrotic effects of action remain unclear. In this study, the anti-fibrotic effects of ATL-1 were examined in fibroblasts, tubular epithelial cells (TECs) triggered by TGF-β1 in vitro, and using a unilateral ureteral obstruction (UUO) mouse model in vivo. We found that ATL-1 represses the myofibroblastic phenotype and fibrosis development in UUO kidneys by targeting the fibroblast-myofibroblast differentiation (FMD), as well as epithelial-mesenchymal transition (EMT). The anti-fibrotic effects of ATL-1 were associated with reduced cell growth in the interstitium and tubules, leading to suppression of the proliferation-linked cascades activity consisting of JAK2/STAT3, PI3K/Akt, p38 MAPK, and Wnt/β-catenin pathways. Besides, ATL-1 treatment repressed TGF-β1-triggered FMD and the myofibroblastic phenotype in fibroblasts by antagonizing the activation of proliferation-linked cascades. Likewise, TGF-β1-triggered excessive activation of the proliferation-linked signaling in TECs triggered EMT. The myofibroblastic phenotype was repressed by ATL-1. The anti-fibrotic and anti-proliferative effects of ATL-1 were linked to the inactivation of Smad2/3 signaling, partially reversing FMD, as well as EMT and the repression of the myofibroblastic phenotype. Thus, the inhibition of myofibroblastic phenotype and fibrosis development in vivo and in vitro through proliferation-linked cascades of ATL-1 makes it a prospective therapeutic bio-agent to prevent renal fibrosis.
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http://dx.doi.org/10.1016/j.bcp.2020.114344DOI Listing
January 2021

Baohuoside I via mTOR Apoptotic Signaling to Inhibit Glioma Cell Growth.

Cancer Manag Res 2020 10;12:11435-11444. Epub 2020 Nov 10.

Neurology Department, Wenzhou Medical University First Affiliated Hospital, Wenzhou, Zhejiang, People's Republic of China.

Introduction: Baohuoside I, a novel oncotherapeutic agent, has been reported to have anti-cancer effects on a variety of cancers, but its role in glioma and its molecular mechanism are still unclear.

Methods: The proliferation of U251 cells was detected by real-time cellular analysis (RTCA), CCK-8, Ki67 immunofluorescence and colony formation assay. The effect of Baohuoside I on the invasion and migration of U251 cells was measured by transwell and scratch tests. The apoptosis of U251 cells was detected by flow cytometry. The expression level of related protein was detected by western blotting.

Results: Baohuoside I could inhibit the proliferation of human glioma cells and induce apoptosis. Further study showed that the migration and invasion ability of glioma was significantly decreased by Baohuoside I. Western blot revealed the expression of p-AMPKα1 protein was up-regulated, and the expression of p-mTOR and p-S6K was down-regulated after Baohuoside I treatment. Tumorigenesis in nude mice showed that Baohuoside I had an anti-glioma effect in vivo.

Conclusion: We propose a natural product, which can inhibit the proliferation, invasion and migration of glioma and may be a valuable anti-tumor candidate. The inhibitory effect of Baohuoside I on the glioma is achieved by inducing the apoptosis of the tumor cells, rather than autophagy. In addition, the pathway to induce cell apoptosis of Baohuoside I is to target the mTOR signal.
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http://dx.doi.org/10.2147/CMAR.S265803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667174PMC
November 2020

Quercetin suppresses pancreatic ductal adenocarcinoma progression via inhibition of SHH and TGF-β/Smad signaling pathways.

Cell Biol Toxicol 2021 06 17;37(3):479-496. Epub 2020 Oct 17.

Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.

Pancreatic ductal adenocarcinoma (PDA) is an aggressive type of malignant tumor with a poor prognosis and high mortality. Aberrant activation of hedgehog signaling plays a crucial role in the maintenance and progression of PDA. Here, we report that the dietary bioflavonoid quercetin has therapeutic potential for PDA by targeting sonic hedgehog (SHH) signaling. The effects of quercetin on the proliferation, apoptosis, migration, and invasion of pancreatic cancer cells (PCCs) and tumor growth and metastasis in PDA xenograft mouse models were evaluated. Additionally, SHH signaling activity was determined. Quercetin significantly inhibited PCC proliferation by downregulating c-Myc expression. In addition, quercetin suppressed epithelial-mesenchymal transition (EMT) by reducing TGF-β1 level, which resulted in inhibition of PCC migration and invasion. Moreover, quercetin induced PCC apoptosis through mitochondrial and death receptor pathways. In nude mouse models, PDA growth and metastasis were reduced by quercetin treatment. Mechanically, quercetin exerts its therapeutic effects on PDA by decreasing SHH activity. Interestingly, quercetin-induced SHH inactivation is mainly dependent on Gli2, but not Gli1. Enhance SHH activity by recombinant Shh protein abolished the quercetin-mediated inhibition of PCC proliferation, migration, and invasion. Furthermore, Shh activated TGF-β1/Smad2/3 signaling and promoted EMT by inducing the expression of Zeb2 and Snail1 that eventually resulted in a partial reversal of quercetin-mediated inhibition of PCC migration and invasion. We conclude that quercetin inhibited the growth, migration, and invasion and induced apoptosis of PCCs by antagonizing SHH and TGF-β/Smad signaling pathways. Thus, quercetin may be a potential candidate for PDA treatment.
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http://dx.doi.org/10.1007/s10565-020-09562-0DOI Listing
June 2021

Experimental kinetic studies on the effects of organic additives on ammonia-based selective non-catalytic reduction process.

Environ Sci Pollut Res Int 2021 Feb 8;28(6):7440-7449. Epub 2020 Oct 8.

Beijing Engineering Research Centre of Process Pollution Control, Key Laboratory of Green Process and Engineering, National Engineering Laboratory for Hydrometallurgical Cleaner Production Technology, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.

The effect of organic components including ethanol, acetic acid, and benzene on ammonia-based selective non-catalytic reduction (SNCR) process has been investigated. Experiments were performed through a quartz tube reactor with simulated flue gas and conducted from 973 to 1373 K. The combustion and pyrolysis properties of organic components were carried out first, it was found that all the combustion processes were completed ahead of the pyrolysis, and the combustion and pyrolysis temperatures for benzene are the highest among the three organic components. Ethanol addition promoted the removal of NO in the temperature range of 973 to 1073 K, and the NO reaction temperature window was broadened, while NO removal was greatly inhibited under a higher temperature over 1100 K. With regard to the effect of addition of multiple organic components on NO removal, the combination of CHO and CHO significantly promoted this process, while the combination of CHO/CHO and CH showed a negative effect on NO removal. The mechanism based on the radicals' reaction has been illustrated, showing the competition of these reactions under different situations.
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http://dx.doi.org/10.1007/s11356-020-10994-8DOI Listing
February 2021

First Report of Powdery Mildew Caused by Podosphaera fusca on Helianthus tuberosus in China.

Plant Dis 2020 Sep 23. Epub 2020 Sep 23.

, China, 476000;

Helianthus tuberosus L. (Jerusalem artichoke) is an herbaceous perennial plant in the Asteraceae that is native to North America but introduced to China in the 17th century. The tubers of H. tuberosus are used as a vegetable, for the pharmaceutical production of inulin and as a source of ethanol biofuel, several B vitamins and minerals。From June to September 2009, severe powdery mildew infection was observed on H. tuberosus in a vegetable garden at Shangqiu Normal University, Shangqiu, China. Approximately 60% of leaves on individual plants were symptomatic, and almost 70% of the plants were infected. Initially, discrete powdery mildew colonies appeared on the upper surface of the leaves and on stems of the plants. Later, mycelial growth was amphigenous, thick, forming irregular white patches, covering the whole leaf surface. Eventually, leaves turned yellow, withered, and abscissed. Microscopic observations showed that conidia on infected leaves were ellipsoid-ovoid to barrel-shaped, with distinct fibrosin bodies visible in their cytoplasm, measuring 28 to 38 × 15 to 22 μm (n = 40). Conidiophores were unbranched, straight, 80 to 210 × 8 to 14 µm (n = 40) in size, and produced two to six immature conidia in chains with intercellular diaphragms. Foot cells of conidiophores were cylindrical and 45 to 65 μm × 8 to 14 µm (n = 40), with slight constrictions at basal septa, and followed by one to three short cells. Fungal hyphae were septate, branched, and flexuous to straight and 5 to 8 µm wide with indistinct to slightly nipple-shaped appressoria. These structures are typical of the genus Podosphaera, although chasmothecia were not observed. The sequence of ITS1-5.8s-ITS2 region of rDNA were amplified from conidia collected from infected leaves with universal primers ITS1F and ITS4 (White et al. 1990), sequenced and analyzed using the BLASTn search of GenBank. Amplicons were 603 bp (GQ927254) and exhibited 99.83% sequence identity with sequence of P. fusca from Cucurbita pepo (KJ698669) in Italy (Pirondi et al. 2015). The fungal species was identified as P. fusca (synonym P. xanthii ) by morphological characteristics and molecular analysis (Braun and Takamatsu 2000; Braun and Cook 2012). Pathogenicity tests were conducted by gently pressing the infected leaves onto leaves of six healthy H. tuberosus plants while six noninoculated plants served as controls. Plants were maintained in a greenhouse at 25 ± 2°C. Eight days after inoculation, symptoms similar to those observed under natural conditions developed on the inoculated leaves of H. tuberosus plants, whereas the control plants remained symptomless. The fungus on inoculated leaves was morphologically identical to that first observed in the field. P. fusca parasitizes a large number of asteraceous species including Euryops pectinatus (Saenz et al. 2000), Coreopsis lanceolata (Garibaldi et al. 2007), Cosmos caudatus (Siddiqui et al. 2011), Herba eupatorii (Ding et al. 2013) and etc. Powdery mildew caused by P. fusca has been reported on H. tuberosus in Russia (Farr and Rossman 2019). Golovinomyces ambrosiae was previously recorded on H. tuberosus in China (Huang et al. 2017; Radisek et al. 2018). This is the first report to our knowledge of powdery mildew P. fusca on H. tuberosus in China. It could cause significant yield losses and become a threat to production of H. tuberosus .
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http://dx.doi.org/10.1094/PDIS-07-20-1488-PDNDOI Listing
September 2020

Corrigendum to "Baohuoside-1 targeting mTOR inducing apoptsis to inhibit hepatocellular carcinoma proliferation, invasion and migration" [Biomed. Pharmacother. 2020 (128) 110366].

Biomed Pharmacother 2020 Oct 19;130:110631. Epub 2020 Aug 19.

Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, The First Affiffiffiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China. Electronic address:

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http://dx.doi.org/10.1016/j.biopha.2020.110631DOI Listing
October 2020

The electron affinity of astatine.

Nat Commun 2020 07 30;11(1):3824. Epub 2020 Jul 30.

CERN, Geneva, Switzerland.

One of the most important properties influencing the chemical behavior of an element is the electron affinity (EA). Among the remaining elements with unknown EA is astatine, where one of its isotopes, At, is remarkably well suited for targeted radionuclide therapy of cancer. With the At anion being involved in many aspects of current astatine labeling protocols, the knowledge of the electron affinity of this element is of prime importance. Here we report the measured value of the EA of astatine to be 2.41578(7) eV. This result is compared to state-of-the-art relativistic quantum mechanical calculations that incorporate both the Breit and the quantum electrodynamics (QED) corrections and the electron-electron correlation effects on the highest level that can be currently achieved for many-electron systems. The developed technique of laser-photodetachment spectroscopy of radioisotopes opens the path for future EA measurements of other radioelements such as polonium, and eventually super-heavy elements.
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http://dx.doi.org/10.1038/s41467-020-17599-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7393155PMC
July 2020

Potential use of king grass (Pennisetum purpureum Schumach. × Pennisetum glaucum (L.) R.Br.) for phytoextraction of cadmium from fields.

Environ Sci Pollut Res Int 2020 Oct 26;27(28):35249-35260. Epub 2020 Jun 26.

College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.

Using king grass (Pennisetum purpureum Schumach. × Pennisetum glaucum (L.) R.Br.) for phytoextraction is a promising technology for producing large amounts of biomass fuel while remediating contaminated soil. To assess the practical phytoextraction capacity of king grass, we conducted a field experiment with three different soil types (loam, sandy loam, clay loam) and cadmium (Cd) concentrations (0, 0.25, 0.5, 1, 2, 4, 8, and 16 mg kg, aged stably for 6 years). King grass were harvested at two different periods (elongation and maturity) to identify the optimal harvest time for extraction efficiency. The results showed that all treatments had bioconcentration factor (BCF) > 1 and translocation factor (TF) < 1; Cd is mainly stored in the roots. However, due to a high shoot biomass, the highest quantity of Cd extracted from shoots was 2.75 mg plant, from the experimental group with 16 mg kg Cd added in sandy loam. A significant positive relationship (P < 0.05) was observed between the amount of Cd extracted from king grass stems, leaves, and roots from soil with the diethylene triamine pentacetate acid (DTPA) extractable Cd concentration. The Cd concentration in shoots at the maturity stage is lower than at the elongation stage, mainly due to the effect of biological dilution. Meanwhile, there is significantly more biomass (P < 0.05) at the maturity stage than at the elongation stage. At the latter, the extraction efficiency of the three soils was loam > sandy loam > clay loam, while at maturity it was sandy loam > clay loam > loam. This change in extraction efficiency can be attributed mainly to differences in soil DTPA-extractable Cd concentration and growth rate caused by differences in soil physical and chemical properties. According to calculations from multiple harvests using three types of soil, remediating contaminated soil with 0-16 mg kg Cd would take 13.9-224.5 and 19.5-250.6 years, extracting 7.21-265.23 and 4.96-330.52 g ha Cd while producing 33.62-66.50 and 73.8-110.5 t ha dry biomass at the elongation (90 days) and maturity (120 days) stages, respectively. In summary, king grass has major potential for remediating Cd-contaminated soil while producing large volumes of biofuel.
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http://dx.doi.org/10.1007/s11356-020-09844-4DOI Listing
October 2020

Corrigendum to "Baohuoside-1 targeting mTOR inducing apoptsis to inhibit hepatocellular carcinoma proliferation, invasion and migration" [Biomed. Pharmacother. 128 (2020) 110366].

Biomed Pharmacother 2020 Sep 17;129:110394. Epub 2020 Jun 17.

Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China. Electronic address:

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http://dx.doi.org/10.1016/j.biopha.2020.110394DOI Listing
September 2020

Precision Landing Test and Simulation of the Agricultural UAV on Apron.

Sensors (Basel) 2020 Jun 14;20(12). Epub 2020 Jun 14.

College of Mechanical and Electronic Engineering, Northwest A&F University, Yangling 712100, China.

Unmanned aerial vehicle (UAV) has been used to assist agricultural production. Precision landing control of UAV is critical for application of it in some specific areas such as greenhouses or livestock/poultry houses. For controlling UAV landing on a fixed or mobile apron/platform accurately, this study proposed an automatic method and tested it under three scenarios: (1) UAV landing at high operating altitude based on the GPS signal of the mobile apron; (2) UAV landing at low operating altitude based on the image recognition on the mobile apron; and (3) UAV landing progress control based on the fixed landing device and image detection to achieve a stable landing action. To verify the effectiveness of the proposed control method, apron at both stationary and mobile (e.g., 3 km/h moving speed) statuses were tested. Besides, a simulation was conducted for the UAV landing on a fixed apron by using a commercial poultry house as a model (135 L × 15 W × 3 H m). Results show that the average landing errors in high altitude and low altitude can be controlled within 6.78 cm and 13.29 cm, respectively. For the poultry house simulation, the landing errors were 6.22 ± 2.59 cm, 6.79 ± 3.26 cm, and 7.14 ± 2.41cm at the running speed of 2 km/h, 3 km/h, and 4 km/h, respectively. This study provides the basis for applying the UAV in agricultural facilities such as poultry or animal houses where requires a stricter landing control than open fields.
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http://dx.doi.org/10.3390/s20123369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349159PMC
June 2020

Baohuoside-1 targeting mTOR inducing apoptsis to inhibit hepatocellular carcinoma proliferation, invasion and migration.

Biomed Pharmacother 2020 Aug 8;128:110366. Epub 2020 Jun 8.

Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China. Electronic address:

Background: Baohuoside-1 is a flavonoid compound isolated from Epimedium koreanum Nakai. This study tried to systematically explore the potential anti-cancer functions of Baohuoside-1 in Hepatocellular Carcinoma and study related molecular mechanism. Moreover, as a potential candidate anti-cancer agent, Baohuoside-1 has relatively low toxic side effect.

Methods: The anti-cancer function including proliferation, invasion and migration of Baohuoside-1 in liver cancer was systematically assessed via colony formation, transwell assay and migration assay. Moreover, the anti-cancer functions of Baohuoside-1 was confirmed based on the nude mouse transplantation tumor experiment. The potential targeted signaling pathway was tested via flow cytometery and western blot analysis.

Results: In this study, we present the anti-HCC activity of Baohuoside-1 isolated from Epimedium through examing the effect of Baohuoside-1 on two different human liver cancer cell lines (HuH-7 and HepG2). Baohuoside-1 significantly inhibited the proliferation, invasion and migration of two liver cancer cell lines. Furthermore, the anticancer activity of Baohuoside-1 was confirmed via inhibiting liver tumor growth in nude mice in vivo. Additionally, the influence of Baohuoside-1 on liver cancer apoptosis was examined by analyzing the expression of pro/anti-apoptotic proteins (BAX, Bcl-2, caspase-3, and caspase-8). The potential targeting signaling of Baohuoside-1 was determined via testing key members' expression changes of mTOR and JAK2 signaling.

Conclusion: The inhibition of liver cancer by Baohuoside-1 is through targeting mTOR signaling not JAK2 signaling to induce apoptosis. Our study indicates that Baohuoside-1 is a potential candidate drug for therapy against liver cancer.
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http://dx.doi.org/10.1016/j.biopha.2020.110366DOI Listing
August 2020

Chemopreventive effect of Betulinic acid via mTOR -Caspases/Bcl2/Bax apoptotic signaling in pancreatic cancer.

BMC Complement Med Ther 2020 Jun 8;20(1):178. Epub 2020 Jun 8.

Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, Wenzhou Medical University First Affiliated Hospital, Wenzhou, Zhejiang, China.

Background: Pancreatic cancer is aggressive with no symptoms until the advanced stage reached. The increased resistance of pancreatic cancer to chemotherapy demonstrates a dilemma in the clinical field. Hence, it is a matter of great urgency to develop an effective drug to treat patients with pancreatic cancer. Betulinic acid is a major triterpene isolated from spina date seed. Several studies have suggested its low toxicity and side effects to patients with malaria and inflammation. However, relevant studies on betulinic acid in inhibiting cancer were insufficient and the molecular mechanism was unclear. This study aimed to systematically explore the potential anti-cancer functions of betulinic acid in pancreatic cancer, and investigate its underlying molecular mechanism.

Methods: The Counting Kit-8 assay, colony formation, transwell invasion assay, wound healing assay, flow cytometry and xenograft nude mice model were used to evaluate the effect of betulinic acid on the proliferation, invasion and migration ability of pancreatic cancer cells.

Results: Our results showed that betulinic acid obviously suppressed pancreatic cancer both in vitro and in vivo in a dose-dependent manner. We also determined that betulinic acid inhibited pancreatic cancer by specifically targeting mTOR signaling rather than Nrf2 or JAK2.

Conclusions: These findings clarify that betulinic acid is a potential and valuable anticancer agent for pancreatic cancer, and indicate the specific molecular target of betulinic acid.
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http://dx.doi.org/10.1186/s12906-020-02976-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7282238PMC
June 2020

A Machine Vision-Based Method for Monitoring Broiler Chicken Floor Distribution.

Sensors (Basel) 2020 Jun 3;20(11). Epub 2020 Jun 3.

Department of Poultry Science, College of Agricultural & Environmental Sciences, University of Georgia, Athens, GA 30602, USA.

The proper spatial distribution of chickens is an indication of a healthy flock. Routine inspections of broiler chicken floor distribution are done manually in commercial grow-out houses every day, which is labor intensive and time consuming. This task requires an efficient and automatic system that can monitor the chicken's floor distributions. In the current study, a machine vision-based method was developed and tested in an experimental broiler house. For the new method to recognize bird distribution in the images, the pen floor was virtually defined/divided into drinking, feeding, and rest/exercise zones. As broiler chickens grew, the images collected each day were analyzed separately to avoid biases caused by changes of body weight/size over time. About 7000 chicken areas/profiles were extracted from images collected from 18 to 35 days of age to build a BP neural network model for floor distribution analysis, and another 200 images were used to validate the model. The results showed that the identification accuracies of bird distribution in the drinking and feeding zones were 0.9419 and 0.9544, respectively. The correlation coefficient (R), mean square error (MSE), and mean absolute error (MAE) of the BP model were 0.996, 0.038, and 0.178, respectively, in our analysis of broiler distribution. Missed detections were mainly caused by interference with the equipment (e.g., the feeder hanging chain and water line); studies are ongoing to address these issues. This study provides the basis for devising a real-time evaluation tool to detect broiler chicken floor distribution and behavior in commercial facilities.
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http://dx.doi.org/10.3390/s20113179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309057PMC
June 2020

Residue behavior and removal of iprodione in garlic, green garlic, and garlic shoot.

J Sci Food Agric 2020 Oct 29;100(13):4705-4713. Epub 2020 Jun 29.

College of Science, China Agricultural University, Beijing, China.

Background: Iprodione is considered to be an endocrine-disturbing pesticide, which could harm consumers. The garlic crop has three edible parts: the garlic, the green garlic, and the garlic shoot, which correspond to different stages of its growth. In this study, iprodione residue dissipation and distribution in these three edible parts were investigated, and dietary risk was evaluated.

Results: Iprodione residues were present in these samples in the following order: green garlic > garlic shoot > > garlic. The dissipation of iprodione in green garlic was slow with a half-life of 5.82-19.25 days. A very high RQ value of 207.35-407.30% suggested that the residual iprodione in green garlic had an unacceptable level of risk. Iprodione residue was significantly eliminated (59-90%) by an alkaline solution. The order for removing iprodione by soaking was the alkaline solutions (0.5% and 2% NaHCO ) > the acidic solutions (5% and 10% of vinegar) ≈ the neutral solutions (the 1% and 2% of table salt) > tap water. Processing factors (PFs) were <1, indicating that processing could decrease the iprodione residue level.

Conclusion: This work could contribute to establishing maximum residue limits (MRLs) for iprodione in garlic, green garlic, and garlic shoots, and could provide guidance on the safe and appropriate use of iprodione in the garlic crop. © 2020 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.10527DOI Listing
October 2020

Optimization of CO Adsorption on Solid-Supported Amines and Thermal Regeneration Mode Comparison.

ACS Omega 2020 May 20;5(17):9641-9648. Epub 2020 Apr 20.

Beijing Engineering Research Centre of Process Pollution Control, Key Laboratory of Green Process and Engineering, National Engineering Laboratory for Hydrometallurgical Cleaner Production Technology, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.

For improving the CO adsorption capacity of solid-supported amines, five commercial porous supports have been selected and impregnated with tetraethylenepentamine (TEPA), and their CO adsorption performances have been evaluated using a fixed-bed reactor coupled with mass spectrometry. For solid-supported amines, CO adsorption capacities coincide with the texture characterization of the adsorbent supports (mesoporous alumina, montmorillonite, silica gel, porous resin, MCM-41 molecular sieve), and the optimum TEPA loading amount is mainly affected by the pore volume. The mesoporous supports were found to be more conducive to uniform loading of organic amine, with more than 370 mg/g CO adsorbed per unit TEPA. Other components in flue gas, especially HO, favor CO adsorption on solid-supported amines. SO inhibited the CO adsorption, which was mainly attributable to the strong and irreversible binding of SO on some amine sites. NO had little effect on CO adsorption. Thermal stabilities of solid-supported amines have been tested based on thermogravimetry curves, and the main weight loss peak for TEPA appears at 513 K for solid-supported amines. Linear and step regeneration modes have been compared, revealing that the temperature for step regeneration is 37 K lower than that for the linear regeneration mode. Moreover, the desorption peak area for the step regeneration mode is 20% higher than that for the linear regeneration mode, indicating that the step regeneration mode can be used in practical applications, to reduce energy consumption during regeneration.
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http://dx.doi.org/10.1021/acsomega.9b03374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7203696PMC
May 2020

Effect of carboxylation cellulose nanocrystal and grape peel extracts on the physical, mechanical and antioxidant properties of konjac glucomannan films.

Int J Biol Macromol 2020 Aug 17;156:874-884. Epub 2020 Apr 17.

College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address:

Active bionanocomposite films were prepared by incorporating konjac glucomannan (KGM) as a matrix, with carboxylation cellulose nanocrystal (C-CNC) as a reinforcement agent and grape peel extracts (GPE) as a natural antioxidation agent. The effects of C-CNC and/or GPE addition on the structural, morphological, barrier, thermal, mechanical and antioxidant properties of the bionanocomposite films were investigated. The rheological results of film forming solutions revealed that C-CNC and GPE were well dispersed in the KGM matrix. Scanning electron micrographs observed the addition of C-CNC had little effect on the microstructure, while more roughness and unevenness were observed on the film surface and cross-section with the C-CNC and GPE. Furthermore, the water vapor barrier property and transparency of the films improved by the addition of the C-CNC and GPE. Notably, the incorporating of C-CNC or GPE significantly alter the mechanical of the KGM/C-CNC/GPE bionanocomposite films. The highest tensile strength was achieved for the KGM/GPE bionanocomposite film with 10 wt% C-CNC, indicating C-CNC and GPE had synergistic effect on enhancing the TS of KGM film. Moreover, the KGM/C-CNC/GPE films exhibited strong antioxidant activity. These results suggested that KGM/C-CNC/GPE bionanocomposite films can be used as an active food packaging for increasing shelf life of packaged foods.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.04.051DOI Listing
August 2020

A network-regulative pattern in the pathogenesis of kidney injury following severe acute pancreatitis.

Biomed Pharmacother 2020 May 12;125:109978. Epub 2020 Feb 12.

Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China; Institute of Kidney Health, Center for Health Assessment, Wenzhou Medical University, Wenzhou, 325000, China. Electronic address:

Severe acute pancreatitis (SAP), a critical inflammatory pathological disease of the pancreas, is crucial for the manifestation of lethal multiple organ dysfunction syndrome and systemic inflammatory response syndrome. Acute kidney injury (AKI) is one of the most severe complications of severe acute pancreatitis. Yet, the specific pathogenesis of AKI following SAP is defectively understood, and involves in multiple pathological processes in a "network-regulative" pattern, including dysfunction of the intestinal barrier, prolonged activation of coagulation, elevated discharge of damage-associated molecular patterns, complication of abdominal compartment syndrome, excessive release of inflammatory mediators, overexpression of procalcitonin, and incitement of chronic metabolic diseases. Therefore, in this review, we summarize the current knowledge on the pathogenesis of kidney injury following SAP to provide a better understanding of the interactions involved and to encourage the identification of novel targeted therapies to treat SAP and associated AKI.
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http://dx.doi.org/10.1016/j.biopha.2020.109978DOI Listing
May 2020
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