Publications by authors named "Yanglin Wu"

3 Publications

  • Page 1 of 1

Melatonin alleviates titanium nanoparticles induced osteolysis via activation of butyrate/GPR109A signaling pathway.

J Nanobiotechnology 2021 Jun 6;19(1):170. Epub 2021 Jun 6.

Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Soochow University, No. 188 Shizi Street, Suzhou, 215006, Jiangsu, China.

Background: Inflammatory osteolysis after total joint replacement (TJR) may cause implant failure, periprosthetic fractures, and be a severe threat to global public health. Our previous studies demonstrated that melatonin had a therapeutic effect on wear-particles induced osteolysis. Gut microbiota is closely related to bone homeostasis, and has been proven to be affected by melatonin. However, whether melatonin could play its anti-osteolysis effects through reprogramming gut microbiota remains elusive.

Results: Here, we demonstrated that melatonin could alleviate Ti-particles induced osteolysis, while this therapeutic effect was blocked by antibiotic cocktail treatment. Interestingly, transplantation of fecal microbiota from mice treated with melatonin reappeared the same beneficial effect. Analysis of the 16S rRNA revealed that melatonin could reverse dysbacteriosis triggered by osteolysis, and elevate the relative abundance of some short chain fatty acid (SCFA) producing bacteria. Moreover, butyrate was enriched by exogenous melatonin administration, while acetate and propionate did not show an evident difference. This was consistent with the results of the metagenomic approach (PICRUSt2) analysis, which revealed a general increase in the synthetic enzymes of butyrate. More importantly, direct supplementation of butyrate could also recapitulate the anti-osteolysis effect of melatonin. Further analysis identified that butyrate alleviated osteolysis via activating its receptor GPR109A, and thus to suppress the activation of NLRP3 inflammasome triggered by Ti-particles.

Conclusions: Taken together, our results suggested that the benefits of melatonin mainly depend on the ability of modulating gut microbiota and regulating butyrate production.
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June 2021

SPECT imaging and highly efficient therapy of rheumatoid arthritis based on hyperbranched semiconducting polymer nanoparticles.

Biomater Sci 2021 Mar;9(5):1845-1854

Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Soochow University, Suzhou 215006, China.

Rheumatoid arthritis (RA) is an inflammatory autoimmune disease. Although significant progress has been made in clinical treatment, joint inflammation may continue or worsen, and may even progress to the end-stage that requires joint replacement. Traditional therapy using methotrexate (MTX) would cause serious off-target systemic toxicities. Therefore, it is crucial to effectively and specifically deliver MTX to targeted inflamed joints to decrease its adverse systemic toxicities and improve its therapeutic index. Herein, we develop multifunctional nanocarriers for diagnostic radioisotope (99mTc) labeling and therapeutic targeted drug (MTX) delivery by using PEGylated hyperbranched semiconducting polymer nanoparticles (HSP-PEG-NPs) as carriers. Upon intravenous administration, the nanoparticles can extravasate through the turbulent blood-joint barrier and access the inflamed joints. In vivo SPECT/CT imaging shows high accumulation in the inflamed joints of mice with RA after intravenous injection of HSP-PEG-NPs with 99mTc labeling (99mTc-HSP-PEG). In vivo therapeutic evaluations suggest that [email protected] significantly alleviate RA with a high therapeutic index and relatively low adverse systemic toxicities in comparison with free MTX at the same dose. Our study shows that HSP-PEG-NPs could serve as multifunctional vehicles to deliver radioisotopes for in vivo imaging, and MTX for RA treatment, highlighting the innovative development of the nanoparticle-based RA treatment strategy for clinical applications.
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March 2021

Formation and evolution of the unexpected PbI2 phase at the interface during the growth of evaporated perovskite films.

Phys Chem Chem Phys 2016 Jul 27;18(27):18607-13. Epub 2016 Jun 27.

School of Materials Science and Engineering, Shanghai University, 99 Shangda Road, Shanghai 200444, China.

The interface chemistry and evolution of the evaporated perovskite films on ITO, pedot/ITO, Si and glass substrates are studied. As evidenced by X-ray diffraction and X-ray photoemission spectroscopy (XPS) results, the PbI2 phase is found to be inevitably formed at the very initial growth stage, even under the conditions of a MAI-rich environment. The extremely low binding energy of adsorbed MAI particles on all the above substrates, as compared to that of PbI2 particles, is responsible for the presence of the PbI2 phase at the interface. The formation of both hole and electron barriers at the interface of PbI2/MAPbI3, as evidenced by XPS measurements, could block carrier transport into the electrode and thus deteriorate solar cell performance. This result reveals the origin of the poor performance of perovskite solar cells (PSCs) by the vacuum evaporation method, and may help to improve the performance of PSCs made using the vacuum evaporation method.
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July 2016