Publications by authors named "Yanghui Cao"

29 Publications

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Application of laparoscopic dual localization of small lesions in radical resection of transverse colon: A video vignette.

Colorectal Dis 2021 Jul 1. Epub 2021 Jul 1.

Department of General Surgery, Affiliated Cancer Hospital of ZhengZhou University, Zhengzhou, China.

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http://dx.doi.org/10.1111/codi.15800DOI Listing
July 2021

A risk signature with inflammatory and immune cells infiltration predicts survival and efficiency of chemotherapy in gastric cancer.

Int Immunopharmacol 2021 Jul 24;96:107589. Epub 2021 May 24.

Department of General Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, China. Electronic address:

Objective: Tumor immune microenvironment biomarkers might add predictive value for outcomes. This study aimed to construct a risk signature with tumor infiltration immune and inflammatory cells to improve the prediction of survival.

Methods: A risk signature model in combination with CD66b + neutrophils, CD3+ T, CD8+ T lymphocytes, and FOXP3 + regulatory T cells was developed in a training cohort of 327 GC patients undergoing surgical resection between 2011 and 2012, and validated in a validation cohort of 285 patients from 2012 to 2013.

Results: The high CD66b expression predicted the poor disease special survival (DSS) and inversely correlated with the CD8 (P < 0.05) and FOXP3 expression (P < 0.05) in the training cohort. This was comparable to the disease-free survival (DFS) findings observed in the validation cohort. Furthermore, a risk stratification was developed from the integration of CD66b + neutrophils and T immune cells. For DFS and DSS, both demonstrated the worse prognosis in the high-risk group, when compared to the low-risk group in both the training cohort and validation cohort (all P < 0.05). In addition, the high-risk group was associated with post-operative relapses, and this risk signature model increased the predictive accuracy and efficiency for post-operative relapses. Moreover, the high-risk group identified a subgroup of GC patients who tended not to benefit from the adjuvant chemotherapy.

Conclusions: The incorporation of neutrophils into T lymphocytes could provide more accurate prognostic information in GC and this risk stratification has potential for identifying the subgroup of GC patients who could benefit from adjuvant chemotherapy.
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http://dx.doi.org/10.1016/j.intimp.2021.107589DOI Listing
July 2021

Chromosome-level genome reference and genome editing of the tea geometrid.

Mol Ecol Resour 2021 Aug 7;21(6):2034-2049. Epub 2021 Apr 7.

CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.

The tea geometrid is a destructive insect pest on tea plants, which seriously affects tea production in terms of both yield and quality and causes severe economic losses. The tea geometrid also provides an important study system to address the ecological adaptive mechanisms underlying its unique host plant adaptation and protective resemblance. In this study, we fully sequenced and de novo assembled the reference genome of the tea geometrid, Ectropis grisescens, using long sequencing reads. We presented a highly continuous, near-complete genome reference (787.4 Mb; scaffold N50: 26.9 Mb), along with the annotation of 18,746 protein-coding genes and 53.3% repeat contents. Importantly, we successfully placed 97.8% of the assembly in 31 chromosomes based on Hi-C interactions and characterized the sex chromosome based on sex-biased sequencing coverage. Multiple quality-control assays and chromosome-scale synteny with the model species all supported the high quality of the presented genome reference. We focused biological annotations on gene families related to the host plant adaptation and camouflage in the tea geometrid and performed comparisons with other representative lepidopteran species. Important findings include the E. grisescens-specific expansion of CYP6 P450 genes that might be involved in metabolism of tea defensive chemicals and unexpected massive expansion of gustatory receptor gene families that suggests potential polyphagy for this tea pest. Furthermore, we developed an efficient genome editing system based on CRISPR/Cas9 technology and successfully implement mutagenesis of a Hox gene in the tea geometrid. Our study provides key genomic resources both for exploring unique mechanisms underlying the ecological adaptation of tea geometrids and for developing environment-friendly strategies for tea pest management.
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http://dx.doi.org/10.1111/1755-0998.13385DOI Listing
August 2021

Application of laparoscopic locating surgical margin in sigmoidectomy-a video vignette.

Colorectal Dis 2021 May 19;23(5):1276-1277. Epub 2021 Feb 19.

Department of General Surgery, Affiliated Cancer Hospital of ZhengZhou University, Zhengzhou, China.

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http://dx.doi.org/10.1111/codi.15560DOI Listing
May 2021

Review of the leafhopper genus Thaia Ghauri and related genera (Hemiptera: Cicadellidae: Typhlocybinae: Erythroneurini).

Zootaxa 2020 Oct 28;4868(3):zootaxa.4868.3.2. Epub 2020 Oct 28.

Key Laboratory of Plant Protection Resources and Pest Management, National Ministry of Education, Entomological Museum, Northwest AF University, Yangling, Shaanxi 712100, P.R.China Illinois Nature History Survey, Prairie Research Institute, University of Illinois at Urbana-Champaign, 1816 S Oak St., Champaign, IL 61820, USA.

The leafhopper genus Thaia Ghauri, 1962 and two related genera, Parathaia Kuoh, 1982 and Pseudothaia Kuoh, 1982, are revised. Nlunga Dworakowska, 1974, previously treated as a subgenus of Thaia, is elevated to the genus level, and Parathaia Kuoh, 1982, previously treated as a synonym of Thaia, is reinstated to valid status. The Oriental species lacking foveae on the pronotum are excluded from Nlunga and transferred to a new genus, Etmaria gen. n. Two species, Pseudothaia caudata Song Li, 2013 and Thaia (Nlunga) leishanensis (Song Li, 2007), are treated as Incertae sedis within Erythroneurini. Moreover, nine additional new species are described and illustrated: Nlunga parareeneni, Etmaria brevis, E. chaiyaphumica, E. dentata, E. indonesica, E. magna, E. triquetra, E. ulterior and Pseudothaia forcipis spp. nov.
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http://dx.doi.org/10.11646/zootaxa.4868.3.2DOI Listing
October 2020

Structure, gene order, and nucleotide composition of mitochondrial genomes in parasitic lice from Amblycera.

Gene 2021 Feb 18;768:145312. Epub 2020 Nov 18.

Department of Entomology, Purdue University, West Lafayette, IN, USA.

Parasitic lice have unique mitochondrial (mt) genomes characterized by rearranged gene orders, variable genome structures, and less AT content compared to most other insects. However, relatively little is known about the mt genomes of Amblycera, the suborder sister to all other parasitic lice. Comparing among nine different genera (including representative of all seven families), we show that Amblycera have variable and highly rearranged mt genomes. Some genera have fragmented genomes that vary considerably in length, whereas others have a single mt chromosome. Notably, these genomes are more AT-biased than most other lice. We also recover genus-level phylogenetic relationships among Amblycera that are consistent with those reported from large nuclear datasets, indicating that mt sequences are reliable for reconstructing evolutionary relationships in Amblycera. However, gene order data cannot reliably recover these same relationships. Overall, our results suggest that the mt genomes of lice, already know to be distinctive, are even more variable than previously thought.
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http://dx.doi.org/10.1016/j.gene.2020.145312DOI Listing
February 2021

Taxonomic study of some Afrotropical erythroneurine leafhoppers (Hemiptera: Cicadellidae: Typhlocybinae).

Zootaxa 2020 Oct 13;4860(3):zootaxa.4860.3.1. Epub 2020 Oct 13.

Key Laboratory of Plant Protection Resources and Pest Management, National Ministry of Education, Entomological Museum, Northwest AF University, Yangling, Shaanxi, CN-712100, P.R.China Illinois Nature History Survey, Prairie Research Institute, University of Illinois at Urbana-Champaign, 1816 S Oak St., Champaign, IL, US-61820, USA.

Six Afrotropical genera of the leafhopper tribe Erythroneurini are redescribed and two new genera, Laminaris gen. nov. and Projecta gen. nov. are established. Sixteen new species are described and illustrated: Laminaris angusta sp. nov., Laminaris cuspidatima sp. nov., Laminaris serrata sp. nov., Laminaris tenuis sp. nov., Projecta auriculata sp. nov., Projecta brevis sp. nov., Projecta depressa sp. nov., Projecta draciformis sp. nov., Accacidia obunca sp. nov., Imbecilla bifurca sp. nov., Imbecilla spinalis sp. nov., Lublinia anchoroides sp. nov., Molopopterus hastata sp. nov., Molopopterus ugandica sp. nov., Lamtoana exigua sp. nov. and Nsesa cameroonica sp. nov. Nsesa Dworakowska, 1974 is newly recorded from Cameroon; Nsimbala Dworakowska, 1974 and Szymczakowskia Dworakowska, 1974 from the Central African Republic; Lublinia Dworakowska, 1970, Lamtoana Dworakowska, 1972 and Ivorycoasta Dworakowska, 1972 from the Republic of Congo. Keys to species of the genera Accacidia Dworakowska, Imbecilla Dworakowska, Lublinia Dworakowska, Molopopterus Jacobi, Lamtoana Dworakowska and Nsesa Dworakowska are also given.
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http://dx.doi.org/10.11646/zootaxa.4860.3.1DOI Listing
October 2020

Circulating miR-1246 Targeting UBE2C, TNNI3, TRAIP, UCHL1 Genes and Key Pathways as a Potential Biomarker for Lung Adenocarcinoma: Integrated Biological Network Analysis.

J Pers Med 2020 Oct 11;10(4). Epub 2020 Oct 11.

Department of Respiratory, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Road, Zhengzhou 450052, China.

Analysis of circulating miRNAs (cmiRNAs) before surgical operation (BSO) and after the surgical operation (ASO) has been informative for lung adenocarcinoma (LUAD) diagnosis, progression, and outcomes of treatment. Thus, we performed a biological network analysis to identify the potential target genes (PTGs) of the overexpressed cmiRNA signatures from LUAD samples that had undergone surgical therapy. Differential expression (DE) analysis of microarray datasets, including cmiRNAs (GSE137140) and cmRNAs (GSE69732), was conducted using the Limma package. cmiR-1246 was predicted as a significantly upregulated cmiRNA of LUAD samples BSO and ASO. Then, 9802 miR-1246 target genes (TGs) were predicted using 12 TG prediction platforms (MiRWalk, miRDB, and TargetScan). Briefly, 425 highly expressed overlapping miRNA-1246 TGs were observed between the prediction platform and the cmiRNA dataset. ClueGO predicted cell projection morphogenesis, chemosensory behavior, and glycosaminoglycan binding, and the PI3K-Akt signaling pathways were enriched metabolic interactions regulating miRNA-1245 overlapping TGs in LUAD. Using 425 overlapping miR-1246 TGs, a protein-protein interaction network was constructed. Then, 12 PTGs of three different Walktrap modules were identified; among them, ubiquitin-conjugating enzyme E2C (UBE2C), troponin T1(TNNT1), T-cell receptor alpha locus interacting protein (TRAIP), and ubiquitin c-terminal hydrolase L1(UCHL1) were positively correlated with miR-1246, and the high expression of these genes was associated with better overall survival of LUAD. We conclude that PTGs of cmiRNA-1246 and key pathways, namely, ubiquitin-mediated proteolysis, glycosaminoglycan binding, the DNA metabolic process, and the PI3K-Akt-mTOR signaling pathway, the neurotrophin and cardiomyopathy signaling pathway, and the MAPK signaling pathway provide new insights on a noninvasive prognostic biomarker for LUAD.
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http://dx.doi.org/10.3390/jpm10040162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712139PMC
October 2020

Cancer-associated fibroblasts promote the migration and invasion of gastric cancer cells via activating IL-17a/JAK2/STAT3 signaling.

Ann Transl Med 2020 Jul;8(14):877

Department of Thoracic Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, China.

Background: Cancer-associated fibroblasts (CAFs), as the activated stroma cells, contribute to tumor progression via the release of cytokines, growth factors, and hormones. However, neither the factors produced by CAFs nor the molecular mechanisms were illuminated very well in gastric cancer (GC).

Methods: Immunohistochemical staining of alpha-smooth muscle actin (α-SMA) was applied to examine the number of CAFs in GC samples from 227 patients. ELISA and qRT-PCR were performed to detect the expression of interleukin 17a (IL-17a). The migration and invasion of GC cells were determined by the Transwell assay. The expressions of JAK2, STAT3, MMP-2, MMP-9, TIMP-1, and TIMP-2 were measured by western blotting. IL-17a was blocked with a polyclonal antibody, and JAK2/STAT3 signaling pathway was blocked by a specific inhibitor AG490.

Results: High CAFs in GC tissues were positively correlated with advanced TNM stage and perineural invasion. Furthermore, GC patients with high CAFs in tumor tissues had an obvious worse disease-free survival (DFS) and disease-special survival (DSS). Multivariate analysis showed that high CAFs in GC tissues were an independent risk factor for DFS and DSS. CAFs expressed IL-17a significantly after GC cell co-culture. CAFs markedly enhanced the migration and invasion abilities of AGS and SGC-7901 cells. Moreover, CAFs co-culture resulted in increased levels of MMP2/9, reduced expressions of TIMP1/2, and activation of the JAK2/STAT3 signaling pathway in the GC cells. IL-17a neutralizing antibody or JAK2 inhibitor AG490 can significantly inhibit the effects of CAFs on the migration, invasion, MMP2/9, TIMP1/2, and JAK2/STAT3 pathways of GC cells.

Conclusions: CAFs correlated with unfavorable clinical features and poor prognosis of GC patients. CAFs secreted IL-17a, which promoted the migration and invasion of GC cells through activating JAK2/STAT3 signaling. These results may identify IL-17a as a promising prognostic marker and therapeutic target of GC.
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http://dx.doi.org/10.21037/atm-20-4843DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396760PMC
July 2020

A timetree for phytoplasmas (Mollicutes) with new insights on patterns of evolution and diversification.

Mol Phylogenet Evol 2020 08 10;149:106826. Epub 2020 Apr 10.

Illinois Natural History Survey, Prairie Research Institute, University of Illinois, Champaign, IL 61820, USA.

The first comprehensive timetree is presented for phytoplasmas, a diverse group of obligate intracellular bacteria restricted to phloem sieve elements of vascular plants and tissues of their hemipteran insect vectors. Maximum likelihood-based phylogenetic analysis of DNA sequence data from the 16S rRNA and methionine aminopeptidase (map) genes yielded well resolved estimates of phylogenetic relationships among major phytoplasma lineages, 16Sr groups and known strains of phytoplasmas. Age estimates for divergences among two major lineages of Mollicutes based on a previous comprehensive bacterial timetree were used to calibrate an initial 16S timetree. A separate timetree was estimated based on the more rapidly-evolving map gene, with an internal calibration based on a recent divergence within two related 16Sr phytoplasma subgroups in group 16SrV thought to have been driven by the introduction of the North American leafhopper vector Scaphoideus titanus Ball into Europe during the early part of the 20th century. Combining the resulting divergence time estimates into a final 16S timetree suggests that evolutionary rates have remained relatively constant overall through the evolution of phytoplasmas and that the origin of this lineage, at ~641 million years ago (Ma), preceded the origin of land plants and hemipteran insects. Nevertheless, the crown group of phytoplasmas is estimated to have begun diversifying ~316 Ma, roughly coinciding with the origin of seed plants and Hemiptera. Some phytoplasma groups apparently associated with particular plant families or insect vector lineages generally arose more recently than their respective hosts and vectors, suggesting that vector-mediated host shifts have been an important mechanism in the evolutionary diversification of phytoplasmas. Further progress in understanding macroevolutionary patterns in phytoplasmas is hindered by large gaps in knowledge of the identity of competent vectors and lack of data on phytoplasma associations with non-economically important plants.
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http://dx.doi.org/10.1016/j.ympev.2020.106826DOI Listing
August 2020

Genomic landscape and genetic manipulation of the black soldier fly Hermetia illucens, a natural waste recycler.

Cell Res 2020 01 25;30(1):50-60. Epub 2019 Nov 25.

CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.

The black soldier fly (BSF), Hermetia illucens (Diptera: Stratiomyidae), is renowned for its bioconversion of organic waste into a sustainable source of animal feed. We report a high-quality genome of 1.1 Gb and a consensus set of 16,770 gene models for this beneficial species. Compared to those of other dipteran species, the BSF genome has undergone a substantial expansion in functional modules related to septic adaptation, including immune system factors, olfactory receptors, and cytochrome P450s. We further profiled midgut transcriptomes and associated microbiomes of BSF larvae fed with representative types of organic waste. We find that the pathways related to digestive system and fighting infection are commonly enriched and that Firmicutes bacteria dominate the microbial community in BSF across all diets. To extend its potential practical applications, we further developed an efficient CRISPR/Cas9-based gene editing approach and implemented this to yield flightless and enhanced feeding capacity phenotypes, both of which could expand BSF production capabilities. Our study provides valuable genomic and technical resources for optimizing BSF lines for industrialization.
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http://dx.doi.org/10.1038/s41422-019-0252-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6951338PMC
January 2020

New erythroneurine leafhopper species from the Australian realm (Hemiptera: Cicadellidae: Typhlocybinae).

Zootaxa 2019 Sep 17;4671(2):zootaxa.4671.2.5. Epub 2019 Sep 17.

Key Laboratory of Plant Protection Resources and Pest Management, National Ministry of Education, Entomological Museum, Northwest AF University, Yangling, Shaanxi 712100, P.R.China Illinois Nature History Survey, Prairie Research Institute, University of Illinois at Urbana-Champaign, 1816 S Oak St., Champaign, IL 61820, USA.

Generic characteristics of three Australian erythroneurine genera are revised based on three new species: Baya lata, Zinga longa and Pettya tenuis spp. nov. Male genitalia characteristics of genus Pettya Kirkaldy are described for the first time.
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http://dx.doi.org/10.11646/zootaxa.4671.2.5DOI Listing
September 2019

Draft genome of the cotton aphid Aphis gossypii.

Insect Biochem Mol Biol 2019 02 24;105:25-32. Epub 2018 Dec 24.

Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Science, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China. Electronic address:

The cotton aphid Aphis gossypii Glover is a worldwide agricultural pest that feeds on cotton, melon, and other landscape plants, causing a high level of economic loss. In addition to the common characteristics shared with other aphids, Ap. gossypii has evolved multiple biotypes that present substantial differences in host adaption. These intriguing biological features are of interest from both a fundamental and applied perspective. However, the molecular studies of Ap. gossypii have been restrained by the lack of a reference genome. Furthermore, in order to establish a platform for the development of novel and sustainable control methods, it is necessary to generate genomic resources for Ap. gossypii. Here, we present a 294 Mb draft genome sequence of Ap. gossypii, which consists of 4,724 scaffolds with an N50 size of 438 kb. Compared to other aphid species with published genomes, Ap. gossypii presents the most compact genome size. A total of 14,694 protein-coding genes were predicted and annotated in the consensus gene set, 98.03% of CEGMA genes and 93.5% of BUSCO genes were captured respectively. Genome-wide selection analyses revealed that significantly evolving pathways in the genus Aphis are related to biological processes of detoxification, steroid biosynthesis, and ethylbenzene degradation. The acquisition of the genome of Ap. gossypii makes it possible to understand the molecular mechanism of intricate biological traits of this species, and will further facilitate the study of aphid evolution.
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http://dx.doi.org/10.1016/j.ibmb.2018.12.007DOI Listing
February 2019

Fall webworm genomes yield insights into rapid adaptation of invasive species.

Nat Ecol Evol 2019 01 10;3(1):105-115. Epub 2018 Dec 10.

CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.

Invasive species cause considerable ecological and economic damage. Despite decades of broad impacts of invasives on diversity and agriculture, the genetic adaptations and near-term evolution of invading populations are poorly understood. The fall webworm, Hyphantria cunea, a highly successful invasive species that originated in North America, spread throughout the Northern Hemisphere during the past 80 years. Here, we use whole-genome sequencing of invasive populations and transcriptome profiling to probe the underlying genetic bases for the rapid adaptation of this species to new environments and host plants. We find substantial reductions in genomic diversity consistent with founder effects. Genes and pathways associated with carbohydrate metabolism and gustatory receptors are substantially expanded in the webworm genome and show strong signatures of functional polymorphisms in the invasive population. We also find that silk-yielding-associated genes maintained a relatively low level of functional diversity, and identify candidate genes that may regulate the development of silk glands in fall webworms. These data suggest that the fall webworm's ability to colonize novel hosts, mediated by plasticity in their gustatory capabilities along with an increased ability to utilize novel nutrition sources and substrates, has facilitated the rapid and successful adaptation of the species throughout its range.
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http://dx.doi.org/10.1038/s41559-018-0746-5DOI Listing
January 2019

Disruption of sex-specific doublesex exons results in male- and female-specific defects in the black cutworm, Agrotis ipsilon.

Pest Manag Sci 2019 Jun 7;75(6):1697-1706. Epub 2019 Jan 7.

Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.

Background: Doublesex (dsx), the downstream gene in the insect sex-determination pathway, is a key regulator of sexually dimorphic development and behavior across a variety of insects. Manipulating expression of dsx could be useful in the genetic control of insects. However, information on the sex-specific function of dsx in non-model insects is lacking.

Results: In this work, we isolated a dsx homolog, which is alternatively spliced into six female-specific and one male-specific isoforms, from an important agricultural pest, the black cutworm, Agrotis ipsilon. Studies on the expression of sex-specific Aidsx mRNA during embryonic development showed that the sixth hour post oviposition is the key stage for sex determination in A. ipsilon. Functional analysis of Aidsx was conducted using a CRISPR/Cas9 system targeting female- and male-specific Aidsx exons. Disruptions of sex-specific Aidsx exons resulted in sex-specific, sexually dimorphic defects in external genitals, gonads and antennae, and expression of sex-specific genes as well as production of offspring in both sexes.

Conclusion: Our results not only demonstrate that dsx is a key player determining A. ipsilon sexually dimorphic traits, but also provide a potential method for the genetic control of this pest. © 2018 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.5290DOI Listing
June 2019

New species of the leafhopper genera Kusala Dworakowska and Diomma Motschulsky (Hemiptera: Cicadellidae: Typhlocybinae: Erythroneurini) with description of a new subgenus.

Zootaxa 2018 Nov 7;4514(2):263-272. Epub 2018 Nov 7.

Key Laboratory of Plant Protection Resources and Pest Management, National Ministry of Education, Entomological Museum, Northwest A&F University, Yangling, Shaanxi 712100, P.R.China.

Generic characteristics of the leafhopper genus Kusala Dworakowska are revised, and a new subgenus Scodela is established. A species checklist of the Kusala is provided and three new species are added: Kusala (Kusala) viraktamathi, K. (Scodela) directa and K. (S.) sinuata spp. nov.. The related genus Diomma Motschulsky is also redescribed and a new species is described: Diomma (Diomma) sangzhiensis sp. nov..
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http://dx.doi.org/10.11646/zootaxa.4514.2.9DOI Listing
November 2018

Review of the leafhopper genus Anufrievia Dworakowska (Hemiptera: Cicadellidae: Typhlocybinae: Erythroneurini).

Zootaxa 2018 Jul 17;4446(2):203-232. Epub 2018 Jul 17.

Key Laboratory of Plant Protection Resources and Pest Management, National Ministry of Education, Entomological Museum, P. O. Box 55#, Northwest A&F University, Yangling, Shaanxi 712100, China.

General characteristics of Anufrievia Dworakowska and a key to the species from China and Thailand are provided. Twenty-one new species, Anufrievia adaucta, A. arcuata, A. curva, A. expensa, A. falcata, A. forcipiformis, A. fusina, A. jinghongensis, A. liubanus, A. parisakazu, A. plana, A. qinlingensis, A. quadrata, A. sphenoides, A. subapicifixa, A. subdentata, A. sufflata, A. symmetrica, A. triangulata, A. triprocessa and A. wolongensis are described. Four species are newly recorded from China: A. akazu (Matsumura), A. badjawae Dworakowska, A. bauhinicola Dworakowska Viraktamath and A. zelta Dworakowska. A. badjawae is also a new record to Thailand.
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http://dx.doi.org/10.11646/zootaxa.4446.2.2DOI Listing
July 2018

The genomic and functional landscapes of developmental plasticity in the American cockroach.

Nat Commun 2018 03 20;9(1):1008. Epub 2018 Mar 20.

CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.

Many cockroach species have adapted to urban environments, and some have been serious pests of public health in the tropics and subtropics. Here, we present the 3.38-Gb genome and a consensus gene set of the American cockroach, Periplaneta americana. We report insights from both genomic and functional investigations into the underlying basis of its adaptation to urban environments and developmental plasticity. In comparison with other insects, expansions of gene families in P. americana exist for most core gene families likely associated with environmental adaptation, such as chemoreception and detoxification. Multiple pathways regulating metamorphic development are well conserved, and RNAi experiments inform on key roles of 20-hydroxyecdysone, juvenile hormone, insulin, and decapentaplegic signals in regulating plasticity. Our analyses reveal a high level of sequence identity in genes between the American cockroach and two termite species, advancing it as a valuable model to study the evolutionary relationships between cockroaches and termites.
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http://dx.doi.org/10.1038/s41467-018-03281-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861062PMC
March 2018

Identification of yellow gene family in Agrotis ipsilon and functional analysis of Aiyellow-y by CRISPR/Cas9.

Insect Biochem Mol Biol 2018 03 11;94:1-9. Epub 2018 Jan 11.

Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China. Electronic address:

The yellow gene family has been identified in several model insects, but yellow genes were poorly identified in non-model insects and the functions of yellow genes are largely unknown. In this study, we identified seven yellow genes in an important agricultural pest Agrotis ipsilon. Each gene encodes a protein containing a major royal jelly domain. Phylogenetic analysis defined these genes as yellow-y, -b, -b2, -c, -d, -e, and -h, respectively. The A. ipsilon yellow genes yellow-b, -b2, and -c were stably expressed in all developmental stages and tissues analyzed, whereas the other four yellow genes had unique expression patterns, suggesting distinct physiological roles of each gene. Using the CRISPR/Cas9 system, we successfully disrupted yellow-y in A. ipsilon and obtained G insects with somatic mutations. Unlike the black of wild-type newly hatched larvae and of adults, the mutants were yellow, although in the pupal stage mutant coloration did not differ from wild-type coloration. This phenotype was inherited by G offspring. The G mutants did not show any growth deficiency compared with control insects; however, a dehydration-like phenotype was observed in newly hatched G larvae from sibling crossed mutants. Our results indicate that A. ipsilon yellow-y gene plays a role in body pigmentation and also might function in waterproofing.
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http://dx.doi.org/10.1016/j.ibmb.2018.01.002DOI Listing
March 2018

[Technical advantages of nano carbon development combined with artery approach in lymph node sorting of rectal cancer].

Zhonghua Wei Chang Wai Ke Za Zhi 2017 Jun;20(6):680-683

Department of Gastrointestinal Surgery, Tumor Hospital of Zhengzhou University, Zhengzhou 450008, China.

Objective: To explore the technical advantages of nano carbon development combined with artery approach in lymph node sorting of rectal cancer.

Methods: From December 2015 to June 2016, 70 patients with of rectal cancer in General Surgery Department of Henan Cancer Hospital were randomly divided into nano carbon development combined with artery approach group(artery approach group) and conventional group. Specimen of artery approach group was placed on the sorting table. Anatomy was performed from the root of inferior mesenteric artery to left colonic artery, sigmoid artery and superior rectal artery. Along the arterial vessel shape, the black-stained lymph nodes and non-stained lymph nodes (perhaps pink, pale yellow, white or pale brown) were examined carefully using visual and haptic combination method for identification of lymph node. From the root of inferior mesenteric artery, central lymph nodes were sorted. Along the vessel shape, vascular lymph nodes were sorted. Intestinal lymph nodes around the rectum were examined as well. Then, specimen was reversed on the sorting table and underwent sorting as above after the examination of obverse. The conventional group received routine method. The total number, the average harvested number, the number of positive lymph nodes and the number of patients with lymph nodes less than 12 were compared between two groups.

Results: Among 70 cases, 37 were male and 33 were female with the median age of 57(32-88) years old. Dixon resection was performed in 46 cases, and Miles resection in 24 cases. Total sorting lymph node was 1 105, including 641 of artery approach group and 464 of control group with significant difference (t=20.717, P=0.000). Lymph node sorting time of artery approach group was (12.6±3.9) minutes, which was shorter than (18.2±4.1) minutes of control group (t=12.464, P=0.000). In artery approach group, number of lymph node with diameter less than 5 mm was 142, sorting rate was 22.2%(142/641), of which 29 were positive(20.4%). In conventional group, 37 lymph nodes with diameter less than 5 mm were found, and sorting rate was 8.0%(37/464), of which 6 were positive(16.2%). Number of the first station of lymph node sorting in artery approach group and conventional group was 282(44%) and 169(36.4%); number of the second station lymph node sorting was 230(35.9%) and 180(38.8%); number of the third station lymph node sorting was 129(20.1%) and 115(24.8%).

Conclusion: The method of nano carbon development combined with artery approach in lymph node sorting of rectal cancer has some advantages, such as simple operation, more harvested lymph nodes, and more accurate pathological staging.
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June 2017

[Application of three-stitch preventive transverse colostomy in anterior resection of low rectal cancer].

Zhonghua Wei Chang Wai Ke Za Zhi 2017 Apr;20(4):439-442

Department of Gastrointestinal Surgery, Tumor Hospital of Zhengzhou University, Zhengzhou 450008, China.

Objective: To explore the application of three-stitch preventive transverse colostomy in anterior resection of low rectal cancer.

Method: From May 2015 to March 2016, 70 consecutive low rectal cancer patients undergoing anterior resection and preventive transverse colostomy in our department were recruited in this prospective study. According to the random number table method, 70 patients were divided into three-stitch transverse colostomy group(observation group, n=35) and traditional transverse colostomy group(control group, n=35). Procedure of three-stitch preventive transverse colostomy was as follows: firstly, at the upper 1/3 incision 0.5-1.0 cm distance from the skin, 7# silk was used to suture from outside to inside, then the needle belt line went through the transverse edge of the mesangial avascular zone. At the lower 1/3 incision 0.5-1.0 cm distance from the skin, 7# silk was used to suture from inside to outside, then silk went through the transverse edge of the mesangial avascular zone again and was ligatured. Finally, in the upper and lower ends of the stoma, 7# silk was used to suture and fix transverse seromuscular layer and the skin. The operation time and morbidity of postoperative complications associated with colostomy were compared between two groups.

Results: There were no significant differences in baseline data between the two groups(all P>0.05). The operative time of observation group was shorter than that of control group [(3.2±1.3) min vs. (15.5±3.4) min, P<0.05]. Incidences of colostomy skin-mucous separation, dermatitis, stoma rebound were significantly lower in observation group [5.7%(2/35) vs. 34.3%(12/35), P=0.007; 8.6%(3/35) vs. 31.4%(11/35), P=0.036; 0 vs. 17.1%(6/35), P=0.025, respectively], while incidences of parastomal hernia and stoma prolapse in two groups were similar (both P>0.05).

Conclusion: Compared with traditional transverse colostomy method, the three-stitch preventive transverse colostomy has more operating advantages and can reduce postoperative complications associated with colostomy.
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April 2017

Hepneriana Dworakowska (Hemiptera: Cicadellidae: Typhlocybinae: Erythroneurini), first record from China, with descriptions of eleven new species.

Zootaxa 2016 Nov 15;4193(2):zootaxa.4193.2.4. Epub 2016 Nov 15.

Key Laboratory of Plant Protection Resources and Pest Management, National Ministry of Education, Entomological Museum, P. O. Box 55#, Northwest A&F University, Yangling, Shaanxi 712100, China. Shaanxi Institute of Zoology, No.88 Xing Qing Ave, Xi'an, Shaanxi 710032, China.

A redescription of the erythroneurine leafhopper genus Hepneriana Dworakowska and a key to Chinese species of the genus are provided. Eleven new species, Hepneriana applanata, H. bicruris, H. concava, H. euryaedeaga, H. longa, H. menglunensis, H. paragamma, H. prostrata, H. robusta, H. taibaoensis and H. undulata spp. nov. are described and H. joannae (Dworakowska) is newly recorded from China.
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http://dx.doi.org/10.11646/zootaxa.4193.2.4DOI Listing
November 2016

Review of the leafhopper genus Alnetoidia Dlabola (Hemiptera: Cicadellidae: Typhlocybinae: Erythroneurini) from China, with descriptions of  two new species.

Zootaxa 2016 Nov 17;4193(3):zootaxa.4193.3.7. Epub 2016 Nov 17.

Key Laboratory of Plant Protection Resources and Pest Management, National Ministry of Education, Entomological Museum, P. O. Box 55#, Northwest A&F University, Yangling, Shaanxi 712100, China..

Generic characteristics of genus Alnetoidia Dlabola are revised based on study of nine species from China, including two new species: A. (Alnetoidia) gracilis, A. (Alnella) dentata spp. nov., and four new records to China. A key to species for identification of Chinese adult males is provided.
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http://dx.doi.org/10.11646/zootaxa.4193.3.7DOI Listing
November 2016

Taxonomic study of the leafhopper genera Gredzinskiya Dworakowska and Musbrnoia Dworakowska (Hemiptera: Cicadellidae: Typhlocybinae: Erythroneurini) with descriptions of seven new species.

Zootaxa 2014 Jan 3;3753:59-70. Epub 2014 Jan 3.

Key Laboratory of Plant Protection Resources and Pest Management, National Ministry of Education, Entomological Museum, P. O. Box 55#, Northwest A&F University, Yangling, Shaanxi 712100, China; Email:

The genera Gredzinskiya Dworakowska and Musbrnoia Dworakowska are revised and seven new species are described: Gredzinskiya lamellaris, G. serrata, G. spinalis, G. zhangae, Musbrnoia angusta, M. corollaris and M. lata spp. nov.. Habitus photos, illustrations of male genitalia and keys to adult males for both genera are provided.
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http://dx.doi.org/10.11646/zootaxa.3753.1.5DOI Listing
January 2014

Review of the leafhopper genus Singapora Mahmood (Hemiptera: Cicadellidae: Typhlocybinae: Erythroneurini).

Zootaxa 2014 Mar 7;3774:333-50. Epub 2014 Mar 7.

Key Laboratory of Plant Protection Resources and Pest Management, National Ministry of Education, Entomological Museum, P. O. Box 55#, Northwest A&F University, Yangling, Shaanxi 712100, China; Email:

General characteristics of Singapora Dworakowska and a key to known species of the genus are provided. Six new species, Singapora candela, S. excedens, S. falcata, S. longiantrosa, S. triacantha and S. yingjiangica spp. nov. are described and illustrated, with two new records, S. shivae Dworakowska from China and S. bannaensis Song & Li from Thailand.
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http://dx.doi.org/10.11646/zootaxa.3774.4.3DOI Listing
March 2014

Review of the leafhopper genus Coloana Dworakowska (Hemiptera: Cicadellidae: Typhlocybinae: Erythroneurini).

Zootaxa 2014 May 27(3802):346-58. Epub 2014 May 27.

Key Laboratory of Plant Protection Resources and Pest Management, National Ministry of Education, Entomological Museum, P. O. Box 55#, Northwest A&F University, Yangling, Shaanxi 712100, China; Email:

General characteristics of Coloana Dworakowska and a key to known species of the genus are provided. Six new species, Coloana accrescens, C. hainanensis, C. latiprocessa, C. orthoprocessa, C. recta and C. sinuata spp. nov. from China and Thailand are described and illustrated. C. cinerea Dworakowska is newly recorded from China.
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http://dx.doi.org/10.11646/zootaxa.3802.3.4DOI Listing
May 2014

Two new erythroneurine leafhopper genera and first record of Thailus Mahmood from China (Hemiptera: Cicadellidae: Typhlocybinae), with description of four new species.

Zootaxa 2013 ;3686:363-72

Key Laboratory ofPlant Protection Resources and Pest Management, National Ministry of Education, Entomological Museum, Northwest A&F University, Yangling, Shaanxi 712100, China.

Two new genera of tribe Erythroneurini from China, Saccata gen. nov. and Variolosa gen. nov. are described and illustrated based on type species Saccata insolita sp. nov. and Variolosa meni sp. nov. respectively. Genus Thailus Mahmood with new species Thailus versicolor sp. nov. is reported from China for the first time and another new species, T rectilinea sp. nov. from Thailand is also described. A key to adult males of Thailus is provided.
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http://dx.doi.org/10.11646/zootaxa.3686.3.5DOI Listing
November 2015

Taxonomic study of the genus Kapsa Dworakowska with a new subgenus, and new combinations and records for Tautoneura Anufriev (Hemiptera: Cicadellidae: Typhlocybinae: Erythroneurini).

Zootaxa 2013 ;3630:117-42

Key Laboratory of Plant Protection Resources and Pest Management, National Ministry of Education, Entomological Museum, P 0. Box 55, Northwest A & F University, Yangling, Shaanxi 712100, China.

The genus Kapsa Dworakowska is redescribed and a new subgenus Kapsa (Rigida) Cao & Zhang is established to include thirteen species, a key to males of the subgenus is provided, and eight new species from China are described and illustrated: Kapsa aculeiformis, K. apicispina, K. brevis, K. explanata, K. furcata, K. imminuta, K. mnegaprocessa and K. serrata spp. nov.. Based on detailed study of previously described species, Kapsa yanheensis Song & Li, 2012 is considered a synonym of Tautoneura albida (Dworakowska, 1970). Four species previously placed in Kapsa are transferred to Tautoneura Anufriev: Tautoneura decorata (Dworakowska, 1981), T. diasonica (Chiang & Knight, 1990), T. elscinta (Chiang & Knight, 1990) and T. puerensis (Song & Li, 2012). K. alba Dworakowska, 1981 and T. ahmedi Dworakowska, 1977 are reported for the first time from China.
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http://dx.doi.org/10.11646/zootaxa.3630.1.4DOI Listing
August 2015

Synonymy, new species and new combinations in the leafhopper genus Matsumurina Dworakowska (Hemiptera: Cicadellidae: Typhlocybinae: Erythroneurini) .

Zootaxa 2013 Nov 7;3734:453-68. Epub 2013 Nov 7.

Key Laboratory of Plant Protection Resources and Pest Management, National Ministry of Education, Entomological Museum, P. O. Box 55#, Northwest A&F University, Yangling, Shaanxi 712100, China; Email:

The leafhopper genus Takama Dworakowska & Viraktamath, 1975 is considered a junior synonym of Matsumurina Dworakowska, 1972, with seven known species all transferred to the latter. Another new combination is proposed: Matsumurina macra (Kuoh, 1982) n. comb. Seven additional species: Matsumurina abbreviata, M. angusta, M. furcata, M. longa, M. longissima, M. qini and M. serriformis spp. nov. are newly described and illustrated. A key for identification of adult males of Matsumurina is provided.
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http://dx.doi.org/10.11646/zootaxa.3734.4.3DOI Listing
November 2013
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