Publications by authors named "Yang Zhao"

2,763 Publications

  • Page 1 of 1

Urinary triclosan in south China adults and implications for human exposure.

Environ Pollut 2021 Jun 9;286:117561. Epub 2021 Jun 9.

School of Public Health (Shenzhen), Sun Yat-sen University, Guangzhou, China. Electronic address:

Triclosan (TCS) is widely applied in personal care products (PCPs) as an antimicrobial preservative. Due to its toxicity and potential risk to human health, TCS has attracted mounting concerns in recent years. However, biomonitoring of TCS in large human populations remains limited in China. In this study, 1163 adults in South China were recruited and urinary TCS concentrations were determined. TCS was detected in 99.5% of urine samples, indicating broad exposure in the study population. Urinary concentrations of TCS ranged from below the limit of detection (LOD) to 270 μg/L, with a median value of 3.67 μg/L. Urinary TCS concentrations from individuals were all lower than the Biomonitoring Equivalents reference dose, suggesting relatively low health risk in the participants. TCS concentrations did not differ significantly between sexes or education levels (p > 0.05). Nevertheless, marital status and age were found to be positively influence TCS levels (p < 0.001). After adjustment for body mass index (BMI), age was determined to be positively associated with TCS concentrations (p < 0.05), particularly in the age group from 31 to 51 years old. This study provides a baseline of urinary TCS exposure in South China general adult populations.
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http://dx.doi.org/10.1016/j.envpol.2021.117561DOI Listing
June 2021

An Overview of the Posttranslational Modifications and Related Molecular Mechanisms in Diabetic Nephropathy.

Front Cell Dev Biol 2021 28;9:630401. Epub 2021 May 28.

Department of Chinese Materia Medica and Natural Medicines, School of Pharmacy, The Air Force Medical University, Xi'an, China.

Diabetic nephropathy (DN), a common diabetic microvascular complication, is characterized by its complex pathogenesis, higher risk of mortality, and the lack of effective diagnosis and treatment methods. Many studies focus on the diagnosis and treatment of diabetes mellitus (DM) and have reported that the pathophysiology of DN is very complex, involving many molecules and abnormal cellular activities. Given the respective pivotal roles of NF-κB, Nrf2, and TGF-β in inflammation, oxidative stress, and fibrosis during DN, we first review the effect of posttranslational modifications on these vital molecules in DN. Then, we describe the relationship between these molecules and related abnormal cellular activities in DN. Finally, we discuss some potential directions for DN treatment and diagnosis. The information reviewed here may be significant in the design of further studies to identify valuable therapeutic targets for DN.
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http://dx.doi.org/10.3389/fcell.2021.630401DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193943PMC
May 2021

The correlation between workers' working pressure and physical and mental health analyzed by the job demand-resource stress model.

Work 2021 Jun 5. Epub 2021 Jun 5.

School of Medicine, York University, Toronto, Canada.

Background: Under the background of the information society, teachers' pressure from work and life is increasing. Meanwhile, the working pressure has a potential inevitable connection with the physical and mental health of teachers.

Objective: To analyze the correlation between working pressure of workers and mental health status, expand the application of the job demand-resource stress (JD-RS) model in the adjustment of working characteristic pressure, and achieve the coordinated development between working pressure and mental health.

Methods: The occupation of the teacher is taken as the research object. First, the pressure source questionnaire and Symptom Check List 90 (SCL-90) are chosen to measure the working pressure and mental health. Also, the reliability and validity of the pressure source questionnaire are tested. Second, the gender, duty, teaching age, and workload of teachers are chosen as the foundation for comparing and analyzing the impact of various dimensions and project factors on teachers' working pressure and mental health. Finally, based on the method of univariate linear regression analysis, the correlation between teachers' working pressure and mental health is analyzed and characterized.

Results: The measurement tool based on the pressure source questionnaire has good performance reliability and validity. The five dimensions of Cronbach's coefficients are all greater than 0.8, and the indicators of fitting all meet psychometrics requirements. Significance analysis shows that different genders, duties, teaching ages, and workloads have different levels of significant influence on teachers' working pressure and mental health. Linear regression analysis shows that teachers' working pressure has a significant impact on their physical and mental health, which has a predictive effect. Teachers who bear high-intensity pressure have psychological problems.

Conclusions: The research based on the JD-RS model has a positive role in promoting the balanced and coordinated development of working pressure and the physical and mental health of employed workers.
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http://dx.doi.org/10.3233/WOR-213500DOI Listing
June 2021

Dihydromyricetin promotes apoptosis, suppresses proliferation and tumor necrosis factor-α-mediated nuclear factor kappa-B activation in nasopharyngeal carcinoma CNE-2 cell.

J Tradit Chin Med 2021 Jun;41(3):367-375

Department of Otolaryngology of the Affiliated Hospital of Guangdong Medical University, Zhanjiang 524023, China.

Objective: To investigate the efficacy of dihydromyricetin (DMY) on nasopharyngeal carcinoma (NPC) cell proliferation, apoptosis and to reveal the underlying mechanism in vitro experiments.

Methods: The CNE-2 cell line was treated with different concentrations of DMY and the effects of DMY on cell viability and proliferation were evaluated using cell counting kit-8 (CCK-8) assay and plate colony formation assay. Cellular apoptosis was detected by flow cytometry following Annexin V fluorescein isothiocyanate/propidine iodide staining. Nuclei morphology was observed under a fluorescence microscope following Hoechst 333258 staining. The expression of phosphorylated inhibitor of nuclear factor kappa-B kinase subunit beta (p-IKKβ), phosphorylated inhibitor of nuclear factor kappa-B kinase subunit alpha (p-IKKα), inhibitor of nuclear factor kappa-B alpha (IκB-α), nuclear factor kappa-B (NF-κB)/p65 was examined by Western blot analysis and the nuclear translocation of NF-κB/p65 was observed using a confocal laser scanning microscopy.

Results: DMY inhibited the proliferative capability and colony formation of NPC CNE-2 cells. Meanwhile, DMY induced apoptosis of CNE-2 cells in a dose and time-dependent manner via upregulating B-cell lymphoma-2 associated X, but downregulating B-cell lymphoma-2 and pro-caspase-3. Importantly, we found that DMY suppressed tumor necrosis factor alpha (TNF-α)-mediated NF-κB activation via inhibiting p-IKKβ, p-IKKα and blocking NF-κB subunit p65.

Conclusion: Our experiments demonstrated that DMY had significant antiproliferative and apoptosisinducing effects on CNE-2 cells. Additionally, DMY promoted inactivation of p-IKKβ, p-IKKα, and blocked the nuclear translocation of NF-κB subunit p65. These results suggest that DMY may be an important therapeutic approach for NPC.
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http://dx.doi.org/10.19852/j.cnki.jtcm.2021.03.004DOI Listing
June 2021

Neuroprotective Effects and Related Mechanisms of Echinacoside in MPTP-Induced PD Mice.

Neuropsychiatr Dis Treat 2021 3;17:1779-1792. Epub 2021 Jun 3.

Department of Neurology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210022, People's Republic of China.

Objective: To explore the neuroprotective effect and the related mechanisms of echinacoside (ECH) in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease (PD) mice.

Methods: Parkinson's disease is induced in mice by MPTP and the neurobehaviors of mice in different groups are observed. Then, immunohistochemistry and Western blot analysis are adopted to measure the expression of tyrosine hydroxylase (TH) and α-synuclein in the substantia nigra (SN). The content of dopamine (DA) and other neurotransmitters in the brain is detected by high-performance liquid chromatography. The expression of nerve growth factors and inflammatory factors in SN in mice in each group is measured by quantitative polymerase chain reaction. Finally, the expression of oxidative stress-related parameters in each group is measured.

Results: Compared with the model group, the pole-climbing time among mice in the moderate and high-dose ECH groups is significantly reduced (P < 0.01). The rotarod staying time, as well as fore and hind-limb strides, shows a significant increase (P < 0.01), as does spontaneous activity (P < 0.01). Moreover, the expression levels of TH, DA, glial cell line-derived neurotrophic factor, and brain-derived neurotrophic factor in SN in mice show significant increases in these two groups (P < 0.01). The content of superoxide dismutase, catalase, and glutathione peroxidase indicates significant increases in the low, moderate, and high-dose ECH groups (P < 0.01), and the content of MDA was reduced (P < 0.01). In the high-dose ECH group, the expression of interleukin (IL) 6 and tumor necrosis factor-α is significantly reduced (P < 0.01), while the expression of IL-10 shows a marked increase (P < 0.01) alongside a decrease in the expression of α-synuclein (P < 0.01).

Conclusion: Echinacoside improves neurobehavioral symptoms in PD mice and significantly increases the expression of TH and DA. The neuroprotective effect potentially correlates with anti-inflammation and anti-oxidation actions, promotes the expression of nerve growth factor, and reduces the accumulation of α-synuclein.
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http://dx.doi.org/10.2147/NDT.S299685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184243PMC
June 2021

The prevalence of multimorbidity and its association with physical activity and sleep duration in middle aged and elderly adults: a longitudinal analysis from China.

Int J Behav Nutr Phys Act 2021 Jun 10;18(1):77. Epub 2021 Jun 10.

Women & Child Health Program, GIC, The George Institute for Global Health at Peking University Health Science Center, Beijing, China.

Background: Preventing chronic disease is important in health policy in countries with significantly ageing populations. This study aims to examine the prevalence of chronic disease multimorbidity and its association with physical activity and sleep duration; and to understand whether physical activity modifies associations between sleep duration and multimorbidity.

Methods: We utilized longitudinal data of a nationally-representative sample from the China Health and Retirement Longitudinal Study (in year 2011 and 2015; N = 5321; 54.7% female; age ≥ 45 years old). Fourteen chronic diseases were used to measure multimorbidity (ten self-reported, and four by blood test). Participants were grouped into high, moderate, and low level based on self-reported frequencies and durations of physical activity with different intensities for at least 10 min at a time in a usual week. Poor and good sleepers were categorized according to average hours of actual sleep at each night during the past month. Panel data method of random-effects logistic regression model was applied to estimate the association of physical activity and sleep with multimorbidity, adjusting for social-demographic and behavioural confounders.

Results: From 2011 to 2015, the prevalence of multimorbidity increased from 52.2 to 62.8%. In 2015, the proportion of participants engaging in high, moderate, and low level of physical activity was 30.3, 24.4 and 45.3%, respectively, and 63.6% of adults had good sleep. For both genders, compared with good sleep, poor sleep was associated with higher odds of multimorbidity (OR = 1.527, 95% CI: 1.277, 1.825). Compared to the high-level group, participants with a low level of physical activity were significantly more likely to have multimorbidity (OR = 1.457, 95% CI: 1.277, 1.825), but associations were stronger among women. The relative excess risk due to interaction between poor sleep and moderate or low physical activity was positive but non-significant on multimorbidity.

Conclusions: The burden of multimorbidity was high in China. Low physical activity and poor sleep was independently and significantly associated with a higher likelihood of multimorbidity in women and both genders, separately. Physical activity could modify the association between sleep and multimorbidity.
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http://dx.doi.org/10.1186/s12966-021-01150-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194125PMC
June 2021

More sensitive to drought of young tissues with weak water potential adjustment capacity in two desert shrubs.

Sci Total Environ 2021 May 29;790:148103. Epub 2021 May 29.

Shapotou Desert Research and Experiment Station, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, 320 Donggang West Road, Lanzhou 730000, China. Electronic address:

Water is the main limiting factor for survival and growth for desert plants, and plants can alleviate water deficits under drought by adjusting water potential (Ψ). However, the water potential adjustment capacity and water-sensitivity at the tissue level among shrub species remains unclear. The objective of this study was to evaluate water potential adjustment capacity and water-sensitivity of different tissues in Artemisia ordosica and Caragana korshinskii through calculating the water relation parameters from pressure-volume (P-V) curves. The present study found that the sensitivity coefficients, -1/β and -1/b, were gradually decreased with increasing degree of lignification in A. ordosica and C. korshinskii, suggesting that younger tissues with low lignification are more sensitive to water deficit. Additionally, the younger tissues with more negative osmotic potential at full turgor (Ψ), water potential at turgor loss point (Ψ), and lower the bulk modulus of elasticity (ε), the relative water deficit at turgor loss point (RWD), apoplastic water fraction (AWF) and total hydraulic capacitance (C), which indicated that younger tissues have stronger turgor adjustment capacity compared to osmotic adjustment capacity and them were more easily lose water during times of decreased water potential because of higher cell wall elasticity and weaker water storage capacity. Collectively, the present study highlighted that younger tissues are more sensitive to drought due to their weaker water potential adjustment capacity and provided critical insight into water physiological mechanism or sensitivity of species to drought.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148103DOI Listing
May 2021

Autoimmune encephalitis associated with anti-LGI1 antibody and Sjögren's syndrome: a case report.

Neurol Sci 2021 Jun 10. Epub 2021 Jun 10.

Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin, China.

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http://dx.doi.org/10.1007/s10072-021-05370-4DOI Listing
June 2021

Solid fuel use and cardiovascular events: A systematic review and meta-analysis of observational studies.

Indoor Air 2021 Jun 10. Epub 2021 Jun 10.

Department of Epidemiology and Health Statistics, College of Public Health, Zhengzhou University, Zhengzhou, China.

Although solid fuel use has been increasingly linked to cardiovascular events (CVEs), conclusions have been inconsistent. We systematically searched 3 databases (PubMed, Embase, and Web of Science) up to July 3, 2020, to identify English language reports that assessed the association of solid fuel use with CVEs. Summary relative risks (RRs) and 95% confidence intervals (CIs) were estimated with a random-effects model. Subgroup analyses and sensitivity analyses were conducted to explore the potential sources of heterogeneity and to test the stability of the results. We finally included 13 observational studies (8 cohort, 3 cross-sectional, and 2 case-control studies comprising 791,220 participants) in the meta-analysis. The risk of CVEs was increased 21% with the highest versus the lowest solid fuel use (highest/lowest, RR  = 1.21, 95% CI: 1.10-1.34). As for the subgroup analyses on study design, the pooled RR for cohort studies, case-control studies, and cross-sectional studies were 1.11 (95%CI: 1.03-1.19), 4.80 (95%CI: 2.22-10.39), and 1.46 (95%CI: 0.82-2.62), respectively. The results of this study suggested that high solid fuel use was associated with increased CVE risk, and that reducing the use of solid fuel will be important for improving the health of the populations in developing countries.
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http://dx.doi.org/10.1111/ina.12867DOI Listing
June 2021

Lithium metal anodes working at 60 mA·cm-2 and 60 mAh·cm-2 through nanoscale lithium-ion adsorbing.

Angew Chem Int Ed Engl 2021 Jun 9. Epub 2021 Jun 9.

Fudan University, Deptm. of Macromolecular Science, 2205 Songhu Road, 200438, Shanghai, CHINA.

Achieving high-current-density and high-area-capacity operation of Li metal anodes offers promising opportunities for high-performing next-generation batteries. However, high-rate Li deposition suffers from undesired Li-ion depletion especially at the electrolyte-anode interface, which compromises achievable capacity and lifetime. Here, electronegative graphene quantum dots are synthesized and assembled into an ultra-thin overlayer capable of efficient Li-ion adsorbing at the nanoscale on Li metal to fully relieve Li-ion depletion. The protected Li anode achieves long-term reversible Li plating/stripping over 1000 h at both superior current density of 60 mA·cm -2 and areal capacity of 60 mAh·cm -2 . Implementation of the protected anode allows for the construction of Li-air full battery with both enhanced rate capability and cycling performance.
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http://dx.doi.org/10.1002/anie.202106047DOI Listing
June 2021

Association of assisted reproductive technology, germline de novo mutations and congenital heart defects in a prospective birth cohort study.

Cell Res 2021 Jun 9. Epub 2021 Jun 9.

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu, China.

Emerging evidence suggests that children conceived through assisted reproductive technology (ART) have a higher risk of congenital heart defects (CHDs) even when there is no family history. De novo mutation (DNM) is a well-known cause of sporadic congenital diseases; however, whether ART procedures increase the number of germline DNM (gDNM) has not yet been well studied. Here, we performed whole-genome sequencing of 1137 individuals from 160 families conceived through ART and 205 families conceived spontaneously. Children conceived via ART carried 4.59 more gDNMs than children conceived spontaneously, including 3.32 paternal and 1.26 maternal DNMs, after correcting for parental age at conception, cigarette smoking, alcohol drinking, and exercise behaviors. Paternal DNMs in offspring conceived via ART are characterized by C>T substitutions at CpG sites, which potentially affect protein-coding genes and are significantly associated with the increased risk of CHD. In addition, the accumulation of non-coding functional mutations was independently associated with CHD and 87.9% of the mutations were originated from the father. Among ART offspring, infertility of the father was associated with elevated paternal DNMs; usage of both recombinant and urinary follicle-stimulating hormone and high-dosage human chorionic gonadotropin trigger was associated with an increase of maternal DNMs. In sum, the increased gDNMs in offspring conceived by ART were primarily originated from fathers, indicating that ART itself may not be a major reason for the accumulation of gDNMs. Our findings emphasize the importance of evaluating the germline status of the fathers in families with the use of ART.
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http://dx.doi.org/10.1038/s41422-021-00521-wDOI Listing
June 2021

Simulation-based training following a theoretical lecture enhances the performance of medical students in the interpretation and short-term retention of 20 cross-sectional transesophageal echocardiographic views: a prospective, randomized, controlled trial.

BMC Med Educ 2021 Jun 9;21(1):336. Epub 2021 Jun 9.

Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, 610041, Sichuan, People's Republic of China.

Background: Both simulation-based training and video-based training serve as educational adjuncts for learning TEE among medical students. In the present study, we hypothesized that simulation-based training would better enhance the performance of medical students in the interpretation of 20 cross-sectional views compared to video-based training.

Methods: A total of 120 4th-year undergraduate medical students were enrolled in the present study. The study began with a pre-test of all the participants, followed by a 90-min theoretical lecture and a post-test. Subsequently, the participants were randomly divided into the video-based group (Group V) and simulation-based group (Group S). Next, Group V received 60 min of TEE video learning, while Group S received 60 min of TEE simulator training. After the respective training, both the groups undertook the retention-test 1 and retention-test 2, 1 week and 1 month later, respectively. The performance for each test was evaluated by five views, which were selected randomly and, respectively, from a set of 20 cross-sectional views. The primary outcome was the performance of the retention-test 1. Secondary outcomes included: (1) comparison the performances of the pre-test, post-test, and retention-test 2 between two groups; (2) comparison the performances of pre-test and post-test in the same group; (3) comparison the performances of retention-test 1, and retention-test 2 in the same group.

Results: Better performances were observed in Group S in both retention-test 1 (Group V: 63.2 [52.6, 77.6] vs. Group S: 89.5 [68.4, 100.0], P < 0.001) and retention-test 2 (Group V: 58.0 [48.0, 72.0] vs. Group S: 74.0 [64.0, 80.0], P < 0.001) compared to Group V. No statistically significant differences were observed in the performances of pre-test (Group V: 8.3 [4.2, 12.5] vs. Group S: 8.3 [4.2, 12.5], P = 0.825) or post-test (Group V: 46.2 [38.5, 57.7] vs. Group S: 44.2 [38.5, 56.7], P = 0.694) between the two groups. The improvement had been observed in the post-test, compared with pre-test in the same group, respectively (Group V in post-test: 46.2 [38.5, 57.7] vs. Group V in pre-test: 8.3 [4.2, 12.5], P < 0.001; Group S in post-test: 44.2 [38.5, 56.7] vs. Group S in pre-test: 8.3 [4.2, 12.5], P < 0.001). However, the performance in retention-test 2 was significantly reduced, compared with retention-test 1 in the same group, respectively (Group V in retention-test 2: 58.0 [48.0, 72.0] vs. Group V in retention-test 1: 63.2 [52.6, 77.6] P = 0.005; Group S in retention-test 2: 74.0 [64.0, 80.0] vs. Group S in retention-test 1: 89.5 [68.4, 100.0], P < 0.001).

Conclusions: Following a 90-min theoretical lecture, simulation-based training better enhanced the performance of medical students in the interpretation and short-term retention of 20 cross-sectional views compared to video-based training.

Trial Registration: http://www.chictr.org.cn ( ChiCTR2000033519 , 3/June/2020).
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http://dx.doi.org/10.1186/s12909-021-02753-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191119PMC
June 2021

A case report of acute simultaneous cardiocerebral infarction: possible pathophysiology.

Ann Palliat Med 2021 May;10(5):5887-5890

Department of Emergency, Affiliated Yancheng Hospital, School of Medicine, Nanjing Medical University, Yancheng, China.

The occurrence of acute myocardial infarction (AMI) accompanied by ischemic cerebrovascular accidents has rarely been reported in previous articles. In this report, we present a 72-year-old female patient with massive cerebral infarction secondary to acute anterior and high lateral wall myocardial infarction, finally resulting in a deep coma. The patient ultimately failed to respond to aggressive resuscitation and succumbed to cardiogenic shock and fatal ventricular arrhythmias. We consider that the co-occurrence of these diseases is more than just a coincidence, and that there may be a connection between them. In this article, we performed an in-depth exploration and discussion of the explanation of this phenomenon. It is essential to recognize these situations in the early stages, which determines the follow-up treatment and prognosis. We suggest that decisions regarding patient management should be based on hemodynamic stability, close cardiac monitoring, and the site of cerebral infarction, and also emphasize that the evaluation of hemodynamic status in these patients is a prerequisite for deciding whether to treat the cerebral or coronary infarction first. The present report is written for the purpose of reminding readers of this rare and severe situation, and to emphasize the necessity for further research on how to deal with it best.
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http://dx.doi.org/10.21037/apm-21-808DOI Listing
May 2021

Simulation of Time- and Frequency-Resolved Four-Wave-Mixing Signals at Finite Temperatures: A Thermo-Field Dynamics Approach.

J Chem Theory Comput 2021 Jun 9. Epub 2021 Jun 9.

School of Science, Hangzhou Dianzi University, Hangzhou 310018, China.

We propose a new approach to simulate four-wave-mixing signals of molecular systems at finite temperatures by combining the multiconfigurational Ehrenfest method with the thermo-field dynamics theory. In our approach, the four-time correlation functions at finite temperatures are mapped onto those at zero temperature in an enlarged Hilbert space with twice the vibrational degrees of freedom. As an illustration, we have simulated three multidimensional spectroscopic signals, time- and frequency-resolved fluorescence spectra, transient-absorption pump-probe spectra, and electronic two-dimensional (2D) spectra at finite temperatures, for a conical intersection-mediated singlet fission model of a rubrene crystal. It is shown that a detailed dynamical picture of the singlet fission process can be extracted from the three spectroscopic signals. An increasing temperature leads to lower intensities of the signals and broadened vibrational peaks, which can be attributed to faster singlet-triplet population transfer and stronger bath-induced electronic dephasing at higher temperatures.
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http://dx.doi.org/10.1021/acs.jctc.1c00259DOI Listing
June 2021

Characterization of the complete chloroplast genome of Baill.

Mitochondrial DNA B Resour 2021 May 27;6(6):1770-1771. Epub 2021 May 27.

Guizhou Academy of Forestry, Guiyang, Guizhou, China.

Baill (Fam.: Leguminosae; Trib.: Caesalpinieae) are widely distributed in China. In this study, we assembled the complete chloroplast (cp) genome of . The total cp genome size was 165,315 bp in length, containing a large single-copy region of 92,356 bp, a small single-copy region of 20,449bp, and a pair of inverted repeat regions of 26,255 bp. The all GC content of cp was 34.95%. It encodes a total of 105 unique genes, including 75 protein-coding genes, 26 tRNA genes, and four rRNA genes. Seventeen genes contain a single intron, and two genes ( and ) have two introns. Phylogenetic analysis results strongly supported that was closely related to
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http://dx.doi.org/10.1080/23802359.2021.1931510DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168727PMC
May 2021

TMK1-based auxin signaling regulates abscisic acid responses via phosphorylating ABI1/2 in .

Proc Natl Acad Sci U S A 2021 Jun;118(24)

FAFU-UCR Joint Center, Horticulture and Metabolic Biology Center, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, People's Republic of China;

Differential concentrations of phytohormone trigger distinct outputs, which provides a mechanism for the plasticity of plant development and an adaptation strategy among plants to changing environments. However, the underlying mechanisms of the differential responses remain unclear. Here we report that a high concentration of auxin, distinct from the effect of low auxin concentration, enhances abscisic acid (ABA) responses in , which partially relies on TRANS-MEMBERANE KINASE 1 (TMK1), a key regulator in auxin signaling. We show that high auxin and TMK1 play essential and positive roles in ABA signaling through regulating ABA INSENSITIVE 1 and 2 (ABI1/2), two negative regulators of the ABA pathway. TMK1 inhibits the phosphatase activity of ABI2 by direct phosphorylation of threonine 321 (T321), a conserved phosphorylation site in ABI2 proteins, whose phosphorylation status is important for both auxin and ABA responses. This TMK1-dependent auxin signaling in the regulation of ABA responses provides a possible mechanism underlying the high auxin responses in plants and an alternative mechanism involved in the coordination between auxin and ABA signaling.
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http://dx.doi.org/10.1073/pnas.2102544118DOI Listing
June 2021

Magnesium Deficiency Causes a Reversible, Metabolic, Diastolic Cardiomyopathy.

J Am Heart Assoc 2021 Jun 5;10(12):e020205. Epub 2021 Jun 5.

Division of Cardiology Department of Medicine The Lillehei Heart InstituteUniversity of Minnesota at Twin Cities Minneapolis MN.

Background Dietary Mg intake is associated with a decreased risk of developing heart failure, whereas low circulating Mg level is associated with increased cardiovascular mortality. We investigated whether Mg deficiency alone could cause cardiomyopathy. Methods and Results C57BL/6J mice were fed with a low Mg (low-Mg, 15-30 mg/kg Mg) or a normal Mg (nl-Mg, 600 mg/kg Mg) diet for 6 weeks. To test reversibility, half of the low-Mg mice were fed then with nl-Mg diet for another 6 weeks. Low-Mg diet significantly decreased mouse serum Mg (0.38±0.03 versus 1.14±0.03 mmol/L for nl-Mg; <0.0001) with a reciprocal increase in serum Ca, K, and Na. Low-Mg mice exhibited impaired cardiac relaxation (ratio between mitral peak early filling velocity E and longitudinal tissue velocity of the mitral anterior annulus e, 21.1±1.1 versus 15.4±0.4 for nl-Mg; =0.011). Cellular ATP was decreased significantly in low-Mg hearts. The changes were accompanied by mitochondrial dysfunction with mitochondrial reactive oxygen species overproduction and membrane depolarization. cMyBPC (cardiac myosin-binding protein C) was -glutathionylated in low-Mg mouse hearts. All these changes were normalized with Mg repletion. In vivo (2-(2,2,6,6-tetramethylpiperidin-1-oxyl-4-ylamino)-2-oxoethyl)triphenylphosphonium chloride treatment during low-Mg diet improved cardiac relaxation, increased ATP levels, and reduced -glutathionylated cMyBPC. Conclusions Mg deficiency caused a reversible diastolic cardiomyopathy associated with mitochondrial dysfunction and oxidative modification of cMyBPC. In deficiency states, Mg supplementation may represent a novel treatment for diastolic heart failure.
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http://dx.doi.org/10.1161/JAHA.120.020205DOI Listing
June 2021

Single-Cell Transcriptomics Reveals Endothelial Plasticity During Diabetic Atherogenesis.

Front Cell Dev Biol 2021 19;9:689469. Epub 2021 May 19.

Frankel Cardiovascular Center, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI, United States.

Atherosclerosis is the leading cause of cardiovascular diseases, which is also the primary cause of mortality among diabetic patients. Endothelial cell (EC) dysfunction is a critical early step in the development of atherosclerosis and aggravated in the presence of concurrent diabetes. Although the heterogeneity of the organ-specific ECs has been systematically analyzed at the single-cell level in healthy conditions, their transcriptomic changes in diabetic atherosclerosis remain largely unexplored. Here, we carried out a single-cell RNA sequencing (scRNA-seq) study using EC-enriched single cells from mouse heart and aorta after 12 weeks feeding of a standard chow or a diabetogenic high-fat diet with cholesterol. We identified eight EC clusters, three of which expressed mesenchymal markers, indicative of an endothelial-to-mesenchymal transition (EndMT). Analyses of the marker genes, pathways, and biological functions revealed that ECs are highly heterogeneous and plastic both in normal and atherosclerotic conditions. The metabolic transcriptomic analysis further confirmed that EndMT-derived fibroblast-like cells are prominent in atherosclerosis, with diminished fatty acid oxidation and enhanced biological functions, including regulation of extracellular-matrix organization and apoptosis. In summary, our data characterized the phenotypic and metabolic heterogeneity of ECs in diabetes-associated atherogenesis at the single-cell level and paves the way for a deeper understanding of endothelial cell biology and EC-related cardiovascular diseases.
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http://dx.doi.org/10.3389/fcell.2021.689469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170046PMC
May 2021

Identification of the hub genes and prognostic indicators of gastric cancer and correlation of indicators with tumor-infiltrating immune cell levels.

J Cancer 2021 13;12(13):4025-4038. Epub 2021 May 13.

Henan Institute of Medical and Pharmaceutical Science, Zhengzhou University, No. 40 Daxue Road, Zhengzhou 450052, China.

To identify the hub genes and prognostic indicators of gastric cancer (GC) and determine the correlation between prognostic indicators and the tumor-infiltrating immune cell levels so as to provide useful information for future GC diagnosis and treatment. The Cancer Genome Atlas (TCGA) stomach adenocarcinoma dataset and two microarray datasets were used to screen the overlapping differentially expressed genes (DEGs) between normal gastric and GC tissue samples. Hub genes were screened via protein-protein interaction networks and module analysis of the overlapping DEGs. Their expression was validated at the cell level and tissue level using the ONCOMINE database. The prognostic indicators of overall survival (OS) and disease-free survival was identified by Cox proportional hazards regression analysis based on tumor grade and cancer stage. The expression of hub genes was validated at the cell level. The correlation of prognostic indicators with the tumor-infiltrating immune cell levels was analyzed using Tumor IMmune Estimation Resource. Ten hub genes, namely , , , , , , , , , and , were screened and their upregulation in the GC tissue was verified. Three prognostic factors, namely , , and , were identified; their expression was higher in GC cells than in normal cells. , , and were correlated with tumor-infiltrating immune cell levels in GC. The identified hub genes and prognostic indicators of GC could be useful indicators for future GC diagnosis and treatment.
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http://dx.doi.org/10.7150/jca.52105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176244PMC
May 2021

Hypoxic microenvironment induced spatial transcriptome changes in pancreatic cancer.

Cancer Biol Med 2021 Jun 4. Epub 2021 Jun 4.

Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China.

Objective: Hypoxia is a significant feature of solid tumors, including pancreatic ductal adenocarcinoma (PDAC). It is associated with tumor invasion, metastasis, and drug resistance. However, the spatial distribution of hypoxia-related heterogeneity in PDAC remains unclear.

Methods: Spatial transcriptomics (STs), a new technique, was used to investigate the ST features of engrafted human PDAC in the ischemic hind limbs of nude mice. Transcriptomes from ST spots in the hypoxic tumor and the control were clustered using differentially-expressed genes. These data were compared to determine the spatial organization of hypoxia-induced heterogeneity in PDAC. Clinical relevance was validated using the Tumor Cancer Genome Atlas and KM-plotter databases. The CMAP website was used to identify molecules that may serve as therapeutic targets for PDAC.

Results: ST showed that the tumor cell subgroups decreased to 7 subgroups in the hypoxia group, compared to 9 subgroups in the control group. Different subgroups showed positional characteristics and different gene signatures. Subgroup 6 located at the invasive front showed a higher proliferative ability under hypoxia. Subgroup 6 had active functions including cell proliferation, invasion, and response to stress. Expressions of hypoxia-related genes, , , and , induced changes. CMAP analysis indicated that ADZ-6482, a PI3K inhibitor, was targeted by the invasive subgroup in hypoxic tumors.

Conclusions: This study is the first to describe hypoxic microenvironment-induced spatial transcriptome changes in PDAC, and to identify potential treatment targets for PDAC. These data will provide the basis for further investigations of the prognoses and treatments of hypoxic tumors.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2021.0158DOI Listing
June 2021

A predictive paradigm for COVID-19 prognosis based on the longitudinal measure of biomarkers.

Brief Bioinform 2021 Jun 3. Epub 2021 Jun 3.

Department of Biostatistics, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China, 211166.

Novel coronavirus disease 2019 (COVID-19) is an emerging, rapidly evolving crisis, and the ability to predict prognosis for individual COVID-19 patient is important for guiding treatment. Laboratory examinations were repeatedly measured during hospitalization for COVID-19 patients, which provide the possibility for the individualized early prediction of prognosis. However, previous studies mainly focused on risk prediction based on laboratory measurements at one time point, ignoring disease progression and changes of biomarkers over time. By using historical regression trees (HTREEs), a novel machine learning method, and joint modeling technique, we modeled the longitudinal trajectories of laboratory biomarkers and made dynamically predictions on individual prognosis for 1997 COVID-19 patients. In the discovery phase, based on 358 COVID-19 patients admitted between 10 January and 18 February 2020 from Tongji Hospital, HTREE model identified a set of important variables including 14 prognostic biomarkers. With the trajectories of those biomarkers through 5-day, 10-day and 15-day, the joint model had a good performance in discriminating the survived and deceased COVID-19 patients (mean AUCs of 88.81, 84.81 and 85.62% for the discovery set). The predictive model was successfully validated in two independent datasets (mean AUCs of 87.61, 87.55 and 87.03% for validation the first dataset including 112 patients, 94.97, 95.78 and 94.63% for the second validation dataset including 1527 patients, respectively). In conclusion, our study identified important biomarkers associated with the prognosis of COVID-19 patients, characterized the time-to-event process and obtained dynamic predictions at the individual level.
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http://dx.doi.org/10.1093/bib/bbab206DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195146PMC
June 2021

Peptide nucleic acid-assisted colorimetric detection of single-nucleotide polymorphisms based on the intrinsic peroxidase-like activity of hemin-carbon nanotube nanocomposites.

Talanta 2021 Sep 20;232:122420. Epub 2021 Apr 20.

School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, 315211, PR China. Electronic address:

Here, taking the advantage of single-stranded (ss) DNA specific nuclease (S1) and peptide nucleic acid (PNA), we demonstrated a novel, rapid, and label-free colorimetric nanosensor for the sensitive and accurate detection of SNPs based on the intrinsic peroxidase-like activity of hemin-functionalized single-walled carbon nanotubes (hemin-SWCNTs). PNA, a man-made mimic of DNA with extraordinary stability toward enzymatic degradation, can effectively protect DNA in the fully matched DNA/PNA duplexes from nuclease digestion. While the DNA in DNA/PNA duplexes containing a mismatch can be cleaved into small fragments. This difference can be visually monitored from the specific color change of TMB/HO system by employing the peroxidase activity of hemin-SWCNTs because of its different aggregation states responding to ssPNA or DNA/PNA duplex. Under optimized conditions, the SNPs in the human tumor suppressor gene TP53 have been successfully genotyped in a linear range of 50-1000 nM with a detection limit of 0.11 nM. Moreover, this platform can effectively discriminate a series of single-base mismatches. This assay avoids the assistance of sophisticated instruments and complicated modifications of probes or nanomaterials, and function well for both cell lysate samples and PCR amplicons from standard cell lines, implying its potential practical applications for bioanalysis and biosensors.
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http://dx.doi.org/10.1016/j.talanta.2021.122420DOI Listing
September 2021

A Heterozygous Genotype-Dependent Branched-Spike Wheat and the Potential Genetic Mechanism Revealed by Transcriptome Sequencing.

Biology (Basel) 2021 May 14;10(5). Epub 2021 May 14.

Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Collaborative Innovation of Modern Crops and Food Crops in Jiangsu, Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Agriculture, Yangzhou University, Yangzhou 225009, China.

Wheat ( L.) spike architecture is an important trait associated with spike development and grain yield. Here, we report a naturally occurring wheat mutant with branched spikelets (BSL) from its wild-type YD-16, which has a normal spike trait and confers a moderate level of resistance to wheat Fusarium head blight (FHB). The lateral meristems positioned at the basal parts of the rachis node of the BSL mutant develop into ramified spikelets characterized as multiple spikelets. The BSL mutant shows three to four-day longer growth period but less 1000-grain weight than the wild type, and it becomes highly susceptible to FHB infection, indicating that the locus controlling the BSL trait may have undergone an intensively artificial and/or natural selection in modern breeding process. The self-pollinated descendants of the lines with the BSL trait consistently segregated with an equal ratio of branched and normal spikelets (NSL) wheat, and homozygotes with the BSL trait could not be achieved even after nine cycles of self-pollination. Distinct segregation patterns both from the self-pollinated progenies of the BSL plants and from the reciprocal crosses between the BSL plants with their sister NSL plants suggested that gametophytic male sterility was probably associated with the heterozygosity for the BSL trait. Transcriptome sequencing of the RNA bulks contrasting in the two types of spike trait at the heading stage indicated that the genes on chromosome 2D may be critical for the BSL trait formation since 329 out of 2540 differentially expressed genes (DEGs) were located on that chromosome, and most of them were down-regulated. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that carbohydrate metabolism may be involved in the BSL trait expression. This work provides valuable clues into understanding development and domestication of wheat spike as well as the association of the BSL trait with FHB susceptibility.
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http://dx.doi.org/10.3390/biology10050437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157103PMC
May 2021

Transcriptome and physiological analyses provide insights into the leaf epicuticular wax accumulation mechanism in yellowhorn.

Hortic Res 2021 Jun 1;8(1):134. Epub 2021 Jun 1.

State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, 100091, Beijing, China.

Plantations and production of yellowhorn, one of the most important woody oil and urban greening trees widely cultivated in northern China, have gradually become limited by drought stress. The epicuticular wax layer plays a key role in the protection of yellowhorn trees from drought and other stresses. However, there is no research on the mechanism of wax loading in yellowhorn trees. In this study, we investigated the anatomical and physiological characteristics of leaves from different germplasm resources and different parts of the same tree and compared their cuticle properties. In addition, the different expression patterns of genes involved in wax accumulation were analyzed, and a coexpression network was built based on transcriptome sequencing data. Morphological and physiological comparisons found that the sun leaves from the outer part of the crown had thicker epicuticular wax, which altered the permeability and improved the drought resistance of leaves, than did shade leaves. Based on transcriptome data, a total of 3008 and 1324 differentially expressed genes (DEGs) were identified between the sun leaves and shade leaves in glossy- and non-glossy-type germplasm resources, respectively. We identified 138 DEGs involved in wax biosynthesis and transport, including structural genes (such as LACS8, ECH1, and ns-LTP) and transcription factors (such as MYB, WRKY, and bHLH transcription factor family proteins). The coexpression network showed a strong correlation between these DEGs. The differences in gene expression patterns between G- and NG-type germplasm resources under different light conditions were very clear. These results not only provide a theoretical basis for screening and developing drought-resistant yellowhorn germplasm resources but also provide a data platform to reveal the wax accumulation process of yellowhorn leaves.
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http://dx.doi.org/10.1038/s41438-021-00564-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167135PMC
June 2021

Clinical value of upfront cranial radiotherapy in osimertinib-treated EGFR-mutant non-small-cell lung cancer with brain metastases.

Int J Radiat Oncol Biol Phys 2021 May 28. Epub 2021 May 28.

Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China; Institute of Thoracic Oncology, Fudan University, Shanghai 200032, China. Electronic address:

Background: As a third-generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI), osimertinib has a powerful ability to penetrate the blood-brain barrier and a high potency for controlling brain metastases (BMs) from EGFR-mutant non-small cell lung cancer (NSCLC). The clinical value of cranial radiotherapy in osimertinib-treated NSCLC with BMs remains largely unknown.

Methods: Patients with NSCLC and BMs and receiving osimertinib treatment as the standard of care were retrospectively enrolled from two institutions. Cranial radiotherapy (whole-brain radiotherapy [WBRT] or/and stereotactic radiosurgery [SRS]) performed before disease progression (PD) to osimertinib was categorized as upfront cranial radiotherapy (ucRT group), excluding those performed during prior EGFR-TKI treatment. Overall survival (OS), progression-free survival (PFS), and time to intracranial progression (iTTP) were compared between the two groups, with adjustment by covariates in propensity-score matched (PSM) analyses. The state of having 1-3 BM lesions, with a maximal size of ≤3 cm was defined as oligo-BMs; otherwise; the cases were defined as multiple BMs.

Results: Of the 205 patients enrolled, osimertinib was used as first-line therapy in 74 and second-line therapy in 131. Forty-eight patients received ucRTs, including WBRT in 24 and SRS in 24. All patients with oligo-BMs in the ucRT group received SRS alone (n=17), while most (28, 90.3%) patients with multiple BMs received WBRT. Failure pattern analyses indicated that in the non-ucRT group, 40.2% of the initial PD involved the brain and 76.9% of the cranial PD involved the original sites, indicating the potential roles of ucRT. Indeed, the iTTP was significantly prolonged (p=0.010) in the ucRT group among the whole population. In the PSM-matched oligo-BM cohort, the ucRT group showed superior PFS (p=0.033) and OS (p=0.026) compared with the non-ucRT group, and the difference remained after multivariate Cox analyses. No such differences were observed in the subpopulation with multiple BMs.

Conclusion: In osimertinib-treated NSCLC patients with BMs, oligo-BM status could be a potential factor to select patients for upfront cranial RT, which warrants further investigation by well-designed clinical trials.
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http://dx.doi.org/10.1016/j.ijrobp.2021.05.125DOI Listing
May 2021

An insulin-independent mechanism for transcriptional regulation of Foxo1 in type 2 diabetic mice.

J Biol Chem 2021 May 28:100846. Epub 2021 May 28.

Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing, China. Electronic address:

Hepatic gluconeogenesis is the major contributor to the hyperglycemia observed in both patients and animals with type 2 diabetes. The transcription factor FOXO1 plays a dominant role in stimulating hepatic gluconeogenesis. FOXO1 is mainly regulated by insulin under physiological conditions, but liver-specific disruption of Foxo1 transcription restores normal gluconeogenesis in mice in which insulin signaling has been blocked, suggesting additional regulatory mechanisms exist. Understanding the transcriptional regulation of Foxo1 may be conducive to the development of insulin-independent strategies for the control of hepatic gluconeogenesis. Here, we found that elevated plasma levels of adenine nucleotide in type 2 diabetes are the major regulators of Foxo1 transcription. We treated lean mice with 5'-AMP and examined their transcriptional profiles using RNA-seq. KEGG analysis revealed that the 5'-AMP treatment led to shifted profiles that were similar to db/db mice. Many of the up-regulated genes were in pathways associated with the pathology of type 2 diabetes including Foxo1 signaling. As observed in diabetic db/db mice, lean mice treated with 5'-AMP displayed enhanced Foxo1 transcription, involving an increase in cellular adenosine levels and a decrease in the S-adenosylmethionine to S-adenosylhomocysteine ratio. This reduced methylation potential resulted in declining histone H3K9 methylation in the promoters of Foxo1, G6Pc, and Pepck. In mouse livers and cultured cells, 5'-AMP induced expression of more FOXO1 protein, which was found to be localized in the nucleus, where it could promoting gluconeogenesis. Our results revealed that adenine nucleotide-driven Foxo1 transcription is crucial for excessive glucose production in type 2 diabetic mice.
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http://dx.doi.org/10.1016/j.jbc.2021.100846DOI Listing
May 2021

Hepatic Arterial Infusion Chemotherapy of Oxaliplatin, Fluorouracil, and Leucovorin With or Without Sorafenib as Initial Treatment for Advanced Hepatocellular Carcinoma.

Front Oncol 2021 12;11:619461. Epub 2021 May 12.

Department of Hepatobiliary Oncology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China.

Purpose: Our previous study showed that hepatic arterial infusion chemotherapy (HAIC) using oxaliplatin, fluorouracil, and leucovorin (FOLFOX) plus sorafenib provided a significant survival benefit over sorafenib for advanced hepatocellular carcinoma. However, it is unclear whether the survival benefit should be attributed to the synergism between HAIC and sorafenib or just HAIC alone. We aim to compare HAIC using FOLFOX plus sorafenib with HAIC alone in patients with advanced hepatocellular carcinoma.

Materials And Methods: This was a retrospective study including 225 eligible patients treated with HAIC using FOLFOX (HAIC alone group, n=126, oxaliplatin 85 mg/m², leucovorin 400 mg/m², fluorouracil bolus 400 mg/m² and 2400 mg/m² for 46 hours, every 3 weeks) alone or HAIC plus sorafenib (soraHAIC group, n=99, sorafenib 400 mg twice daily). Survival curves were calculated by the Kaplan-Meier method, and propensity-score matching was used to reduce bias.

Results: The soraHAIC group showed a longer overall survival (12.9 [95% CI, 10.4-15.4] vs. 10.5 [95% CI, 9.5-11.5] months, HR=0.71 [95% CI, 0.53-0.96]; =0.025), a better progression free survival (7.0 [95% CI, 5.3-8.8] vs. 5.3 [95% CI, 3.5-7.1] months, HR=0.76 [95% CI, 0.58-0.99]; =0.046), and a higher disease control rate (RECIST 1.1: 74.8% vs. 61.1%, =0.030) than the HAIC alone group. In multivariate analysis, soraHAIC was an independent favor factor for survival. In terms of the grade 3/4 adverse event, hand-foot skin reaction was more frequent in the soraHAIC group than the HAIC alone group. In the propensity-score matched cohorts (93 pairs), the overall survival, the progression free survival and disease control rates in the soraHAIC group were also better than those in the HAIC group (<0.05).

Conclusion: HAIC plus sorafenib may improve overall survival and progression free survival compared with HAIC alone as initial treatment for advanced hepatocellular carcinoma.
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http://dx.doi.org/10.3389/fonc.2021.619461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149911PMC
May 2021

Exceptional High and Reversible Ammonia Uptake by Two Dimension Few-layer BiI Nanosheets.

ACS Appl Mater Interfaces 2021 Jun 28;13(22):25918-25925. Epub 2021 May 28.

Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Key Laboratory of Green Chemistry, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, School of Chemistry and Chemical Engineering, Henan Normal University, 46 Jianshe Road, Xinxiang, Henan 453007, P. R. China.

The emission of NH into atmosphere is seriously harmful for human health and public safety, thus the capture and recovery of NH from ammonia emissions is highly desirable. In recent years, many kinds of solid adsorbents have been exploited to absorb NH. However, these materials do not show the advantages of high uptake capacity and good recyclability at the same time. Here, nontoxic and low cost few-layer BiI nanosheets have been prepared from bulk BiI powder by a simple and efficient liquid phase exfoliation strategy using green solvents and then applied for the NH capture for the first time. The results show that the adsorption capacity of NH of BiI nanosheets reaches up to 22.6 mmol/g at 1.0 bar and 25 °C, which approaches the record value for NH adsorption. Importantly, the NH uptake in BiI nanosheets is completely reversible and no clear loss in uptake capacity is observed after 10 cycles of adsorption-desorption. Furthermore, the BiI nanosheets exhibit remarkable selectivity for the separation of NH/CO at 70 °C with theoretical selectivity coefficient of 700, which is promising for the selective separation of NH and CO in hot tail gas of some industrial processes. Mechanism studies indicate that such superior NH capacity, excellent reversibility and remarkable selectivity are primarily attributed to the Bi-NH coordination interactions.
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http://dx.doi.org/10.1021/acsami.1c03261DOI Listing
June 2021

Radical treatment of severe open fractures of extremities by orthoplastic surgery: a 10-year retrospective study.

J Orthop Surg Res 2021 May 27;16(1):340. Epub 2021 May 27.

Department of Orthopeadic Surgery, Xijing Hospital, Air Force Military Medical University, No. 127 Changle Xi Road, Xi'an, Shaanxi Province, 710032, People's Republic of China.

Objective: This study aimed to retrospectively analyze clinical data of a series of patients with severe open fractures of extremities (Gustilo IIIb or IIIc), who achieved a satisfactory outcome through radical orthoplastic surgery, so as to provide a reference for determining the treatment of severe open fractures of extremities.

Methods: The clinical data of 41 consecutive patients with severe open fracture (Gustilo IIIb or IIIc) of the limb, who underwent successful surgical debridement, fixation, and soft tissue reconstruction in one stage between January 2008 and January 2019, were retrospectively reviewed. Postoperative indicators, including infection rate and union time, were acquired by a regular follow-up and analyzed.

Results: The mean (±SD) age of the patients was 38 ± 16 years. A total of 90 open fractures and severe soft tissue damages were analyzed. The soft tissue cover was achieved within 72 h. The overall rate of infection was 14.6% (6/41). Sex and the Mangled Extremity Severity Score were associated with infection. The median union time of 40 patients (one amputation) was 32 weeks.

Conclusion: The overall rate of infection exhibited a lower tendency in this study compared with previous studies on high-grade open fractures following a two-stage orthopedic approach. The consequence of infection rate and union time was similar to that in previous studies. These results indicated that the single-stage radical orthoplastic treatment was an effective and reliable option for reconstructing severe open fractures.
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http://dx.doi.org/10.1186/s13018-021-02479-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157635PMC
May 2021

Screening for mutants with altered Ca signal response using aequorin-based Ca reporter system.

STAR Protoc 2021 Jun 23;2(2):100558. Epub 2021 May 23.

Shanghai Center for Plant Stress Biology, and CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China.

Environmental stimuli evoke transient increases of the cytosolic Ca level. To identify upstream components of Ca signaling, we have optimized two forward genetic screening systems based on Ca reporter aequorin. AEQ and AEQ plants were used for generating ethyl methanesulfonate (EMS)-mutagenized libraries. The AEQ EMS-mutagenized library was preferably used to screen the mutants with reduced Ca signal response due to its high effectiveness, while the AEQ EMS-mutagenized library was used for screening of the mutants with altered Ca signal response. For complete details on the use and execution of this protocol, please refer to Chen et al. (2020) and Zhu et al. (2013).
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http://dx.doi.org/10.1016/j.xpro.2021.100558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144734PMC
June 2021