Publications by authors named "Yang Zhang"

5,079 Publications

  • Page 1 of 1

Alprostadil for hypertensive nephropathy: A systematic review and meta-analysis of randomized controlled trials.

PLoS One 2022 26;17(5):e0269111. Epub 2022 May 26.

Infectious Disease Department, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan Province, People's Republic of China.

We performed a meta-analysis to evaluate the efficacy of alprostadil in the treatment of hypertensive nephropathy. Seven online databases (PubMed, Embase, Cochrane Library, China National Knowledge Infrastructure [CNKI] database, Wanfang Data Knowledge Service Platform, VIP Information Resource Integration Service Platform [cqVIP], and China Biology Medicine Disc [SinoMed]) were searched from inception to January 31, 2022, and a set of clinical indicators for hypertensive nephropathy was selected. The main indicators were 24-h urinary protein, serum creatinine, endogenous serum creatinine clearance rate, blood urea nitrogen, cystatin C, and mean arterial pressure. The methodological quality of the included trials was analyzed using a risk of bias assessment according to the Cochrane Manual guidelines, and a meta-analysis was performed. A random-effects model was implemented to pool the results. A total of 20 randomized controlled trials involving 1441 patients with hypertensive nephropathy were included in this review. Our findings showed that alprostadil had a positive effect on 24-h urinary protein (mean difference [MD] = -0.79, 95% confidence interval [CI] [-1.16, -0.42], P < 0.0001), serum creatinine (MD = -13.83, 95% CI [-19.34, -8.32], P < 0.00001), endogenous serum creatinine clearance rate (MD = 6.09, 95% CI [3.59, 8.59], P < 0.00001), blood urea nitrogen (MD = -6.42, 95% CI [-8.63, -4.21], P < 0.00001), cystatin C (MD = -0.26, 95% CI [-0.34, -0.18], P < 0.00001), and mean arterial pressure levels(MD = -13.65, 95% CI [-16.08, -11.21], P < 0.00001). Compared to conventional treatment alone, alprostadil combined with conventional treatment can improve renal function in patients with hypertensive nephropathy more effectively. However, additional large-scale, multicenter, rigorously designed randomized controlled trials are needed to verify these results. This is the first meta-analysis to evaluate the efficacy of alprostadil for hypertensive nephropathy, and the results may guide clinical practice.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0269111PLOS
May 2022

Refractory Childhood Glaucoma Secondary to Weill-Marchesani Syndrome. Case Management with Combined CO Laser-Assisted Sclerectomy Surgery and Trabeculectomy.

Chin Med Sci J 2022 May 16. Epub 2022 May 16.

Department of Ophthalmology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China.

A 2-year-old girl was diagnosed as Weill-Marchesani syndrome with typical systemic features of short stature, short and stubby hands and feet,language disorders and mental retardation, who developed bilateral angle closure glaucoma, ectopia lentis and suffered visual loss from the ocular features of Weill-Marchesani syndrome. The child was successfully treated by combined CO laser-assisted sclerectomy surgery and trabeculectomy.
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http://dx.doi.org/10.24920/003956DOI Listing
May 2022

GLP-1 Analog Alleviated Cognitive Dysfunction in Aged Rats Anaesthetized with Sevoflurane.

Biomed Res Int 2022 16;2022:9339824. Epub 2022 May 16.

Department of Endocrinology and Metabolism, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.

Postoperative cognitive dysfunction (POCD) in elderly patients undergoing general anesthesia is a major problem in the aging society. Sevoflurane is the most widely applied anesthetic in clinical practice. In this study, we investigated the effects of the GLP-1 analogue liraglutide on cognitive function in aged rats anesthetized by sevoflurane. Specifically, 48 Sprague-Dawley rats were divided into the control (C) group, the liraglutide (L) group, the sevoflurane (S) group, and the sevoflurane+liraglutide (SL) group, each group with 12 rats. In the S group and the SL group, the rats were injected subcutaneously with normal saline and liraglutide after inhalation of a mixture of 3% sevoflurane and pure oxygen. In the C group and the L group, normal saline and liraglutide were injected subcutaneously into the rats after inhalation of pure oxygen. Morris Water Maze Task was applied for the detection of spatial learning and memory in rats; HE and TUNEL for staining; and western blot for quantifying Bax, Bcl-2 expression, and examining caspase-3 activity in hippocampal tissues as well as for revealing the antiapoptotic mechanism. Besides, the accumulation of inflammatory factors NF-B and IL-1 in the hippocampal tissue was quantitatively studied to reveal the anti-inflammatory mechanism. The protective effect of liraglutide on sevoflurane toxicity was the first to be confirmed in this study. Additionally, this study elucidated the mechanism of the above effect. The results of this study might be helpful to find an effective medical solution for the treatment of POCD caused by sevoflurane anesthesia.
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http://dx.doi.org/10.1155/2022/9339824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9126689PMC
May 2022

After-hour elective total knee arthroplasty does not affect clinical outcomes but negatively affects alignment.

Arch Orthop Trauma Surg 2022 May 25. Epub 2022 May 25.

Division of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, 1838 Guangzhou Avenue, Guangzhou, 510515, Guangdong, China.

Introduction: In cases of limited medical resources, elective total knee arthroplasty (TKA) sometimes needs to be performed after typical work hours. However, surgeon fatigue and logistical factors may potentially affect outcomes. This study aimed to detect whether after-hour procedures impair outcomes after TKA.

Materials And Methods: Elective unilateral TKA from Jan 1, 2016 to Nov 31, 2018 was retrospectively selected and separated into two groups. Procedures started from 8:00 A.M. to 5:29 P.M. were identified as day-time surgeries, whereas those started from 5:30 P.M. to 11:59 P.M. were considered after-hour surgeries. Operative period, Knee Society Score (KSS), range of motion (ROM), total blood loss, length of hospital stay (LOS), and postoperative adverse events and complications were compared. Additionally, the components were evaluated radiologically.

Results: A total of 321 patients were selected, including 258 (80.37%) patients in the day-time group and 63 (19.63%) patients in the after-hour group. Operative period, LOS, total blood loss were similar between groups. The overall and each specific incidence of postoperative complications were comparable between the two groups, but the incidence of postoperative vomiting (POV) was higher in the after-hour group. There was no significant difference in knee joint function as shown by the KSS and ROM, both on the 3rd day and at 2 years after surgeries. Radiologically, there were no significant differences between the two groups in the femoral notches (P = 0.592). However, better coronal alignment was detected in the day-time group (P = 0.002), consistent with which there were less outliers (P = 0.033).

Conclusion: After-hour TKA procedure does not exert an impact on clinical outcomes, but negatively affects lower limb alignment. Besides, after-hour TKA surgery impairs patients' comfort by increasing POV.
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http://dx.doi.org/10.1007/s00402-022-04490-6DOI Listing
May 2022

A Disposable Nitinol Memory Alloy Anal Fistula Clip (AFC) for the Treatment of Cryptoglandular Fistula-In-Ano: a Prospective, Randomized, Controlled Study With Short-Term Follow-Up.

J Gastrointest Surg 2022 May 25. Epub 2022 May 25.

National Colorectal Disease Center of Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210022, Jiangsu Province, China.

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http://dx.doi.org/10.1007/s11605-022-05355-4DOI Listing
May 2022

IDH mutation and cancer stem cell.

Essays Biochem 2022 May 25. Epub 2022 May 25.

Neuro-Oncology Branch, Center for Cancer Research, National Cancer Institute, MD 20892, U.S.A.

Cancer stem cells (CSCs) are a small population of cells in human malignancies that resemble the biology of human pluripotent stem cells. CSCs are closely related to the critical hallmarks in human cancers, ranging from oncogenesis to disease progression, therapeutic resistance, and overall outcome. Mutations in isocitrate dehydrogenase (IDH) were recently identified as founder mutations for human cancers. An increasing amount of evidence indicates that IDH mutations are closely related to the establishment and maintenance of CSCs. Biosynthesis of oncometabolite, metabolic reprogramming, and epigenetic shifts establish distinctive molecular signatures in IDH-mutated CSCs. Additionally, IDH mutation and IDH-related pathways could be valuable molecular targets to impact the CSC components in human cancers and to improve the disease outcome.
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http://dx.doi.org/10.1042/EBC20220008DOI Listing
May 2022

[Using plant extracts and their active ingredients to inhibit bacterial biofilms].

Sheng Wu Gong Cheng Xue Bao 2022 May;38(5):1753-1767

Immunology Research Center, Medical Research Institute, Southwest University, Chongqing 402460, China.

Numerous studies have reported that the resistance of biofilm bacteria to antibiotics can be up to 10-1 000 fold higher than that of planktonic bacteria. Bacterial biofilms are reported to be responsible for more than 80% of human microbial infection, posing great challenges to the healthcare sector. Many studies have reported that plant extracts and their active ingredients can inhibit the formation and development of bacterial biofilms, including reducing biofilm biomass and the number of viable bacteria in biofilms, as well as eradicating mature biofilms. This review summarized the plant extracts and their active ingredients that are inhibitory to bacterial biofilms, and analyzed the underpinning mechanisms. This review may serve as a reference for the development of plant drugs to prevent and treat biofilm infections.
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http://dx.doi.org/10.13345/j.cjb.210561DOI Listing
May 2022

Glutamate excitotoxicity: Potential therapeutic target for ischemic stroke.

Biomed Pharmacother 2022 May 20;151:113125. Epub 2022 May 20.

Department of Cardiovascular, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China. Electronic address:

Glutamate-mediated excitotoxicity is an important mechanism leading to post ischemic stroke damage. After acute stroke, the sudden reduction in cerebral blood flow is most initially followed by ion transport protein dysfunction and disruption of ion homeostasis, which in turn leads to impaired glutamate release, reuptake, and excessive N-methyl-D-aspartate receptor (NMDAR) activation, promoting neuronal death. Despite extensive evidence from preclinical studies suggesting that excessive NMDAR stimulation during ischemic stroke is a central step in post-stroke damage, NMDAR blockers have failed to translate into clinical stroke treatment. Current treatment options for stroke are very limited, and there is therefore a great need to develop new targets for neuroprotective therapeutic agents in ischemic stroke to extend the therapeutic time window. In this review, we highlight recent findings on glutamate release, reuptake mechanisms, NMDAR and its downstream cellular signaling pathways in post-ischemic stroke damage, and review the pathological changes in each link to help develop viable new therapeutic targets. We then also summarize potential neuroprotective drugs and therapeutic approaches for these new targets in the treatment of ischemic stroke.
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http://dx.doi.org/10.1016/j.biopha.2022.113125DOI Listing
May 2022

Innate immune mediator, Interleukin-1 receptor accessory protein (IL1RAP), is expressed and pro-tumorigenic in pancreatic cancer.

J Hematol Oncol 2022 May 23;15(1):70. Epub 2022 May 23.

Albert Einstein College of Medicine, Montefiore Medical Center, 1300 Morris Park Avenue, Bronx, NY, 10461, USA.

Advanced pancreatic ductal adenocarcinoma (PDAC) is usually an incurable malignancy that needs newer therapeutic targets. Interleukin-1 receptor accessory protein (IL1RAP) is an innate immune mediator that regulates activation of pro-inflammatory and mitogenic signaling pathways. Immunohistochemistry on tissue microarrays demonstrated expression of IL1RAP in majority of human PDAC specimens and in murine pancreatic tumors from K-Ras/p53/PDXCre (KPC) mice. Single cell RNA-Seq analysis of human primary pre-neoplastic lesions and adenocarcinoma specimens indicated that overexpression occurs during carcinogenesis. IL1RAP overexpression was associated with worse overall survival. IL1RAP knockdown significantly reduced cell viability, invasiveness, and clonogenic growth in pancreatic cancer cell lines. Inhibition of the downstream interleukin-1 receptor-associated kinase 4 (IRAK4) using two pharmacologic inhibitors, CA-4948 and PF06650833, resulted in reduced growth in pancreatic cancer cell lines and in xenograft models.
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http://dx.doi.org/10.1186/s13045-022-01286-4DOI Listing
May 2022

Metagenomic Next-Generation Sequencing for Accurate Diagnosis of Acute HIV Infection with Aseptic Meningitis: A Case Report.

Infect Drug Resist 2022 16;15:2529-2536. Epub 2022 May 16.

Department of Infectious Disease, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, Zhejiang, 321000, People's Republic of China.

Background: Although individuals infected with HIV for the first time manifest a series of acute syndromes, most patients show mild or no symptoms, which complicates the initial clinical diagnosis. Early diagnosis is important for effective prevention and management of patients. Metagenomic next-generation sequencing technology (mNGS) can rapidly detect a wide range of pathogenic microorganisms, even in atypical cases. However, to date, few studies have reported the application of mNGS to diagnose acute HIV infection with aseptic meningitis.

Case Presentation: A 38-year-old man was admitted to the Department of Infectious Diseases due to repeated fever, headache, and scattered rashes on his limbs. Routine blood analysis revealed elevated absolute lymphocytes and monocytes. Moreover, monocytes were found to be significantly increased following a lumbar puncture and cerebrospinal fluid detection. mNGS results revealed the presence of the human immunodeficiency virus (HIV-1), with HIV RNA of 910 copies/mL in his cerebrospinal fluid. The HIV antigen/antibody test was negative. According to a study by Fie Big et al, a clear diagnosis of acute HIV infection at Fiebig stage I. The patient's condition improved after treatment, and he was prescribed antiretroviral therapy (ART) after discharge.

Conclusion: Aseptic meningitis is easily misdiagnosed during the initial stages of acute HIV infection. mNGS can be used to identify the pathogen early, rapidly, and accurately, thereby improving the treatment of acute HIV infections.
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http://dx.doi.org/10.2147/IDR.S361049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122666PMC
May 2022

Identification of excretory and secretory proteins from Haemonchus contortus inducing a Th9 immune response in goats.

Vet Res 2022 May 21;53(1):36. Epub 2022 May 21.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.

Th9 cells have been shown to play crucial roles in anti-parasite immunity, pathogenic microbe infection, and allergy. Previous studies have demonstrated that Haemonchus contortus excretory and secretory proteins (HcESPs) induce the proliferation of Th9 cells and alter the transcriptional level of IL-9 as well as its related pathways in the Th9 immune response after infection. However, the exact molecule(s) in HcESPs inducing the Th9 immune response is not yet known. In this study, flow cytometry, co-immunoprecipitation (Co-IP) and shotgun liquid chromatography tandem-mass spectrometry (LC-MS/MS) were used, and a total of 218 proteins from HcESPs that might interact with goat Th9 cells were identified. By in vitro culture of Th9 cells with HcESPs, 40 binding proteins were identified. In vivo, 38, 47, 42 and 142 binding proteins were identified at 7, 15, 35 and 50 days post-infection (dpi), respectively. Furthermore, 2 of the 218 HcESPs, named DNA/RNA helicase domain containing protein (HcDR) and GATA transcription factor (HcGATA), were confirmed to induce the proliferation of Th9 cells and promote the expression of IL-9 when incubated with goat peripheral blood mononuclear cells (PBMCs). This study represents a proteomics-guided investigation of the interactions between Th9 cells and HcESPs. It provides a new way to explore immunostimulatory antigens among HcESPs and identifies candidates for immune-mediated prevention of H. contortus infection.
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http://dx.doi.org/10.1186/s13567-022-01055-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123704PMC
May 2022

Integrin α3/α6 and αV are implicated in ADAM15-activated FAK and EGFR signalling pathway individually and promote non-small-cell lung cancer progression.

Cell Death Dis 2022 May 21;13(5):486. Epub 2022 May 21.

Department of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Soochow University, Suzhou, 215006, China.

Disintegrin-metalloproteinase 15(ADAM15), a member of disintegrin metalloproteinases (ADAMs), plays important roles in various cancer types. However, the underlying ADAM15 functioning in lung cancer is still unclear. In the present study, we find that ADAM15 regulates the epidermal growth factor receptor/focal adhesion kinase (EGFR/FAK) signalling pathway by interactions with integrins. Integrin αV is involved in ADAM15-mediated FAK signalling. Further, we find that ADAM15 and CD151 were co-expressed, and the presence of ADAM15 affected the integrin α3/α6-related EGFR signalling pathway by cooperating with CD151. In addition, we also prove the effect of ADAM15 on proliferation in nude mice. Finally, we show that ADAM15 is a direct target of miR-204-5p by luciferase reporter assays, qRT-PCR and western blot analyses. Our findings provide molecular and cellular evidence that ADAM15 promotes cell proliferation and metastasis in NSCLC, which might provide a potential target for NSCLC treatment.
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http://dx.doi.org/10.1038/s41419-022-04928-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124216PMC
May 2022

Sulfated Cyclocarya paliurus polysaccharides improve immune function of immunosuppressed mice by modulating intestinal microbiota.

Int J Biol Macromol 2022 May 18;212:31-42. Epub 2022 May 18.

Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China. Electronic address:

The study was aimed to investigate the effect of Cyclocarya paliurus polysaccharides (CPP) and the sulfation derivative (S-CPP) on modulate intestinal mucosal immunity and intestinal microbiota in cyclophosphamide-induced mice. The results showed that CPP and S-CPP effectively alleviated intestinal villi injury, enhanced the contents of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in small intestinal tissue and serum, and upregulated IL-1β at gene levels, zonula occludens-1 (ZO-1), Occludin and Claudin-1 at gene and protein levels, thereby promoting the repair of intestinal mechanical barrier and enhancing intestinal mucosal immunity. Moreover, the beneficial modulation of CPP and S-CPP on the overall structure of intestinal microbiota was revealed by performing 16S ribosomal RNA (16S rRNA) sequencing. Sulfated modification could improve the protection of CPP on the intestinal barrier and the regulation of systemic immunity. S-CPP had a stronger potential to reduce the damage of cyclophosphamide (Cy) on immunity and intestinal microbiota.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.05.110DOI Listing
May 2022

Response of bacterial communities and nitrogen-cycling genes in newly reclaimed mudflat paddy soils to nitrogen fertilizer gradients.

Environ Sci Pollut Res Int 2022 May 20. Epub 2022 May 20.

Jiangsu Key Laboratory of Crop Genetic and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Research Institute of Rice Industrial Engineering Technology, Yangzhou University, Yangzhou, 225009, China.

Conversion of coastal mudflats into paddy soils is an effective measure to alleviate the pressures on land resources. However, few studies have evaluated the effects of nitrogen (N) fertilizers on bacterial communities in newly reclaimed mudflat paddy soils. We performed a field plot experiment with six N fertilizer rates (0, 210, 255, 300, 345, and 390 kg N ha) in a newly reclaimed mudflat paddy for 2 consecutive years and used Illumina sequencing and qPCR to investigate the effects of N fertilizers on bacterial communities and N-cycling genes. Results showed that high N fertilization (above 300 kg N ha) increased the contents of organic matter (OM), total N (TN), ammonium (NH), and nitrate (NO) and significantly decreased the diversity and richness of bacteria. Furthermore, high N fertilization had a stronger effect on bacterial communities than low N fertilization, probably due to high concentrations of NH, OM, and NO. Additionally, in paddy soils with high N fertilizer application, the relative abundances of Bacteroidetes, γ-proteobacteria, and Actinobacteria increased significantly, but the reverse was true for those of Chloroflexi, Firmicutes, δ-proteobacteria, α-proteobacteria, Acidobacteria, and β-proteobacteria. The results of qPCR indicated that high N fertilization significantly increased the relative abundance of nifH genes involved in N fixation and decreased that of amoA-archaea involved in ammonia oxidation, nirS genes involved in nitrite reduction, and nosZ genes involved in nitrous oxide reduction, which suggested that high N fertilization increased the potential of available N retention and reduced the potential of nitrous oxide emission. Overall, N fertilizers with an N fertilizer rate of above 300 kg N ha significantly altered the bacterial communities and N-cycle of mudflat paddy soils.
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http://dx.doi.org/10.1007/s11356-022-20770-5DOI Listing
May 2022

Large-scale single-cell encapsulation in microgels through metastable droplet-templating combined with microfluidic-integration.

Biofabrication 2022 May 19. Epub 2022 May 19.

Harvard School of Engineering and Applied Sciences, Harvard University, Pierce Hall, 29 Oxford Street, Cambridge, MA 02138, Cambridge, Massachusetts, MA 02138, UNITED STATES.

Current techniques for the generation of cell-laden microgels are limited by numerous challenges, including poorly uncontrolled batch-to-batch variations, processes that are both labor- and time-consuming, the high expense of devices and reagents, and low production rates; this hampers the translation of laboratory findings to clinical applications. To address these challenges, we develop a droplet-based microfluidic strategy based on metastable droplet-templating and microchannel integration for the substantial large-scale production of single cell-laden alginate microgels. Specifically, we present a continuous processing method for microgel generation by introducing amphiphilic perfluoronated alcohols to obtain metastable emulsion droplets as sacrificial templates. In addition, to adapt to the metastable emulsion system, integrated microfluidic chips containing 80 drop-maker units are designed and optimized based on the computational fluid dynamics simulation. This strategy allows single cell encapsulation in microgels at a maximum production rate of 10 ml hr-1 of cell suspension while retaining cell viability and functionality. These results represent a significant advance toward using cell-laden microgels for clinical-relevant applications, including cell therapy, tissue regeneration and 3D bioprinting.
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http://dx.doi.org/10.1088/1758-5090/ac7168DOI Listing
May 2022

Isolated-Oxygen-Vacancy Hardening in Lead-Free Piezoelectrics.

Adv Mater 2022 May 20:e2202558. Epub 2022 May 20.

Y.-X. Liu, H.-C. Thong, T. N. Nguyen, J.-W. Li, Prof. J.-F. Li, and Prof. K. Wang, State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, 100084, P. R. China.

Defect engineering is a well-established approach to customize the functionalities of perovskite oxides. In demanding high-power applications of piezoelectric materials; acceptor doping serves as the state-of-the-art hardening approach but inevitably deteriorates the electromechanical properties. Here; we propose a new hardening effect associated with the isolated oxygen vacancy for achieving well-balanced performances. Guided by theoretical design; a well-balanced performance of mechanical quality factor (Q ) and piezoelectric coefficient (d ) is achieved in lead-free potassium sodium niobate ceramics; where Q increases by over 60% while d remains almost unchanged. By atomic-scale Z-contrast imaging; hysteresis measurement; and quantitative piezoresponse force microscopy analysis; we reveal that the improved Q results from the inhibition of both extrinsic and intrinsic losses while the unchanged d is associated with the retainment of polarization contributes. More encouragingly; the hardening effect shows exceptional stability with increasing vibration velocity; offering the potential in material designing for practical high-power applications; such as pharmaceutical extraction and ultrasonic osteotome. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202202558DOI Listing
May 2022

Relationship Between Four Non-Insulin-Based Indexes of Insulin Resistance and Serum Uric Acid in Patients with Type 2 Diabetes: A Cross-Sectional Study.

Diabetes Metab Syndr Obes 2022 9;15:1461-1471. Epub 2022 May 9.

Department of Endocrinology, Tianjin First Center Hospital, Tianjin, 300192, People's Republic of China.

Aim: The aim of this study was to investigate the association between serum uric acid (SUA) levels and four insulin resistance surrogates in patients with type 2 diabetes (T2DM). The four non-insulin-based indexes of insulin resistance (IR) include the glucose and triglycerides index (TyG), TyG index with body mass index (TyG-BMI), ratio of triglycerides to high-density lipoprotein cholesterol (TG/HDL-c) and metabolic score for insulin resistance (METS-IR).

Methods: A total of 687 patients with T2DM were enrolled in the current study. Patients were stratified into three groups according to their levels of SUA. Spearman correlation was used to analyze the correlation between SUA and clinical variables. Multiple linear regression analysis was used to assess the association between SUA and the four insulin resistance surrogates. Receiver operating characteristic (ROC) analyses and the area under the ROC curve (AUC) were then used to assess the ability of TyG, TyG-BMI, TG/HDL-c, and METS-IR to discriminate hyperuricemia (HUA) in T2DM.

Results: SUA in T2DM was significantly positively correlated with TyG (r 0.406 P < 0.01), TyG-BMI (r 0.272 P < 0.01), TG/HDL-c (r 0.493 P < 0.01), and METS-IR (r 0.238 P < 0.01). Furthermore, higher values of the four insulin resistance surrogates were independently correlated with higher SUA levels in T2DM patients (P < 0.01 for all) after adjusting for confounding factors. TyG, TyG-BMI, TG/HDL-c, and METS-IR all had a significant discriminative ability for HUA in patients with T2DM. The AUC values were 0.693 (95% CI 0.645-0.741), 0.649 (95% CI 0.599-0.699), 0.768 (95% CI 0.726-0.811), and 0.660 (95% CI 0.609-0.710), respectively.

Conclusion: The present study suggests that TyG, TyG-BMI, TG/HDL-c and METS-IR had a significant correlation with SUA in T2DM. TG/HDL-c was the best marker among the four insulin resistance surrogates for the identification of HUA in T2DM.
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http://dx.doi.org/10.2147/DMSO.S362248DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113036PMC
May 2022

Correction: Influence of frailty and its interaction with comorbidity on outcomes among total joint replacement.

BMC Musculoskelet Disord 2022 May 19;23(1):478. Epub 2022 May 19.

Division of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, 1838 Guangzhou Avenue, Guangzhou, 510515, Guangdong, China.

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http://dx.doi.org/10.1186/s12891-022-05425-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121550PMC
May 2022

Rutin Inhibits the Progression of Osteoarthritis Through -Mediated Signaling.

DNA Cell Biol 2022 May 19. Epub 2022 May 19.

Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Osteoarthritis (OA) is a chronic joint disease characterized by the deterioration of cartilage and subchondral bone in the joints. Currently, there is no complete cure for OA, only treatments designed to temporarily relieve pain and improve function. Compared with the high cost of surgical treatment, medical treatment of OA is more acceptable and cost-effective. Rutin, as a flavonoid, has been shown to have anti-OA properties. We evaluated the effects of rutin on chondrocytes in lipopolysaccharide (LPS)-induced OA and on OA in rats induced by anterior cruciate ligament transection. We found that rutin effectively reduced the expression levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), and matrix metalloproteinase 13 (MMP-13) and increased the expression of Col II and aggrecan ( < 0.001). In addition, we also found that rutin increased the expression of cystathionine-β-synthase (CBS) and inhibited the expression of Rho-related coiled-coil protein kinase (ROCK) in chondrocytes ( < 0.05), thereby effectively inhibiting the inflammatory progression of OA. We concluded that rutin inhibits the inflammatory progression of OA through the -mediated signaling pathway.
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http://dx.doi.org/10.1089/dna.2021.1182DOI Listing
May 2022

Assessing the attitude and problem-based learning in mathematics through PLS-SEM modeling.

PLoS One 2022 19;17(5):e0266363. Epub 2022 May 19.

School of Education, Shaanxi Normal University, Xi'an, P.R. China.

Mathematics plays a leading part in day-to-day life and has enhanced a necessary component for human accomplishments. Students from many countries do not reach the expected level in mathematics. Therefore, it is essential to pay close consideration to the causes related to ability in mathematics. Mathematics attitude is considered as one of the critical variables in the process of mathematics learning. This study aimed to determine students' attitudes and achievements through problem-based learning in mathematics. The selected study group contained 600 students and 35 teachers from rural public secondary schools in District Rawalpindi, Pakistan. The data collection was done using questionnaires from students and teachers and collected data analyzed by SPSS 23 and Amos 23. This study's result was carried out using Partial Least square structural equation Model (PLS-SEM), descriptive analysis, and hypotheses testing. The outcomes in this study indicated that the mean fluctuated between 1 to 4.5, 3.71 to 4.20, and Std. Deviation fluctuated between 0.6 to 2.0 and 0.75 to 1.55 in the students and teacher models, respectively. The results of the PLS-SEM students' model show a negative attitude towards mathematics. The teachers' PLS-SEM model showed the Effects of using problem-based learning (PBL) on students' achievements. According to the hypotheses testing, the acceptance of hypotheses by stating that the Confidence in Learning Mathematics Scale (C), Value of Mathematics Scale (V), and Student Mathematics Motivation Scale (M) are significant effects for the Students' Attitude Toward Problem-Based Learning (ATPBL). But the Attitude Toward Enjoyment in Mathematics Scale (AE) was rejected, and it did not significantly affect the ATPBL. As well as, the Problem-solving learning and students' achievement (PLA), Advantages of problem-solving learning (APL) and Difficulties in using problem-solving learning (DPL) have a significant positive effect on the ATPBL. Finally, this study suggested that teachers also adopt new teaching methods corresponding to mathematics, and there is a need to explore particular mathematics skills to enhance students' learning abilities.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0266363PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9119563PMC
May 2022

Nonahybrids that consisit of p-type, nitrogen-doped ZnO and graphene nanostructures: synthesis, photophysical properties, and biosensing application.

Nanotechnology 2022 May 18. Epub 2022 May 18.

Hangzhou Dianzi University, Hangzhou Dianzi University, Hangzhou, 310018, CHINA.

ZnO, a promising material for optoelectronic applications, has attracted considerable attention due to its wide and direct band gap and large exciton binding energy. To understand the applications of this material, fabrication of high quality p-type ZnO is a key step. However, a reliable p-type doping of this material remains a major challenge. In this study, we report p-type nitrogen-doped ZnO nanoparticle, grown in a nitrogen doped graphene layer matrix by a plasma heating process using a natural protein and zinc nitrate as the precursors. The structural characterizations are developed by several microscopic techniques including the field emission electron microscopy (FE-SEM), high resolution transmission electron microscopy (HR-TEM), X-ray photoelectron spectroscopy (XPS), and micro-Raman analysis. In addition, the ultraviolet (UV)-visible absorption characteristics and photoluminescence properties of the samples are studied. Its p-type conduction behaviour is confirmed by the Hall effect measurement, which was ascribed to the high nitrogen dopant concentration in the Zn-poor ZnO, and the related mechanism for the p-type behaviour is also discussed. Moreover, the results of the glucose detection based on the strong green luminescence of glucose indicate that the nitrogen-doped ZnO nanodots/nitrogen-doped graphene layer nanohybrid is also a competitive candidate in the biosensing field.
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http://dx.doi.org/10.1088/1361-6528/ac70e4DOI Listing
May 2022

Dangling Octahedra Enable Edge States in 2D Lead Halide Perovskites.

Adv Mater 2022 May 18:e2201666. Epub 2022 May 18.

School of Materials Science and Engineering, Tianjin Key Laboratory of Metal and Molecule-Based Material Chemistry, Nankai University, Tianjin, 300350, China.

The structural reconstruction at the crystal layer edges of 2D lead halide perovskites (LHPs) leads to unique edge states (ES) which are manifested by prolonged carrier lifetime and reduced emission energy. These special ES could effectively enhance the optoelectronic performance of devices, but their intrinsic origin and working mechanism remain elusive. Here we demonstrate that the ES of a family of 2D Ruddlesden-Popper LHPs [BA CsPb Br , BA MAPb Br and BA MA Pb Br (BA = butylammonium; MA = methylammonium)] arise from the rotational symmetry elevation of the PbBr octahedra dangling at the crystal layer edges. These dangling octahedra give rise to localized electronic states that enable an effective transport of electrons from the interior to layer edges, and the population of electrons in both the intrinsic states and ES can be manipulated via controlling the external fields. Moreover, the abundant phonons, activated by the dangling octahedra, can interact with electrons to facilitate radiative recombination, counterintuitive to the suppressive role commonly observed in conventional semiconductors. This work unveils the intrinsic atomistic and electronic origins of ES in 2D LHPs, which could stimulate the exploration of ES-based exotic optoelectronic properties and corresponding design of high-performance devices for these emergent low-dimensional semiconductors. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202201666DOI Listing
May 2022

Dual nanoenzymes loaded hollow mesoporous organotantalum nanospheres for chemo-radio sensitization.

J Control Release 2022 May 18;347:369-378. Epub 2022 May 18.

Guangzhou Key Laboratory of Tumor Immunology Research, Cancer Research Institute, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China; Experimental Education/Administration Center, School of Basic Medical Science, Southern Medical University, Guangzhou 510515, China. Electronic address:

Chemo-radiotherapy has been extensively used in clinics, displaying substantial advantages in treatment and prognosis. Stimuli-responsive biodegradable nanoagents that can achieve not only delivery and controlled release of chemotherapeutics, but also hypoxia alleviation to enhance chemoradiotherapy therefore has tremendous potential. Herein, glutathione (GSH)-responsive, biodegradable, doxorubicin-carrying hollow mesoporous organotantalum nanospheres modified with Au and Pt dual nanoenzymes ([email protected]@[email protected]) were constructed for chemo-radio sensitization. Degradation of [email protected]@[email protected] can be self-activated through GSH stimulation and on-demand release packaged Dox owing to the disulfide bond in the hybrid framework of organotantalum nanospheres. Au and Pt nanoenzymes triggered cascade catalytic reactions that could alleviate hypoxia by utilizing β-d-glucose and HO, thereby sensitizing ROS-based chemoradiotherapy with synergistic starving therapy. Given the radiosensitization of high-Z elements (Ta, Pt, Au), nanoenzymes induced cascade catalytic reaction for hypoxia relief, and the depletion of the predominant antioxidant GSH, desirable tumor suppression could be achieved both in vitro and in vivo, indicating that [email protected]@[email protected] is a promising nanoagent to boost chemo-radiotherapy.
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http://dx.doi.org/10.1016/j.jconrel.2022.05.018DOI Listing
May 2022

Programming a Metal-Organic Framework toward Excellent Hypergolicity.

ACS Appl Mater Interfaces 2022 May 16. Epub 2022 May 16.

Henan Key Laboratory of Crystalline Molecular Functional Materials, Green Catalysis Center and College of Chemistry, Zhengzhou University, Zhengzhou 450001, China.

Exploring novel hypergolic fuels for modern space propulsion is highly desired. However, the analysis and understanding of the structure and hypergolic performance at the molecular level are still insufficient. To understand the factors that dictate hypergolicity, we conducted a comparative study on a series of metal-organic frameworks (MOFs) characterized by the same topology but with varied ligand structures. The ignition delay (ID) time trend was found to be imidazole < triazole < tetrazole, and the rapid ID time was 8 ms. By combining experimental studies and density functional theory (DFT) calculations, we found that propargyl and cyanoborohydride groups that functioned as dual hypergolic triggers contributed to the hypergolicity, and a distinct electronic structure was detrimental to ID time. The structure-performance relationships presented herein can potentially provide some fundamental insights into the field of developing high-performance hypergolic fuels.
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http://dx.doi.org/10.1021/acsami.2c05252DOI Listing
May 2022

Simultaneous determination of abiraterone and its five metabolites in human plasma by LC-MS/MS: Application to pharmacokinetic study in healthy Chinese subjects.

J Pharm Biomed Anal 2022 May 10;217:114826. Epub 2022 May 10.

Clinical Trial Center, Zhongnan Hospital of Wuhan University, Wuhan, China. Electronic address:

In this study, a rapid, simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously quantify abiraterone (ABI), a widely used anti-metastatic castration-resistant prostate cancer drug, and its metabolites comprising Δ-abiraterone (D4A), 3-keto-5α-abiraterone (5αA), abiraterone N-oxide (A-NO), abiraterone sulfate (A-Sul) and abiraterone N-oxide sulfate (A-NO-Sul) in human plasma. The analytes were extracted by protein precipitation with acetonitrile and ideal chromatographic separation was achieved on ACE-C18 column (2.1 × 50 mm, 5 µm) using a gradient elution. Triple Quad™ 6500 mass spectrometer equipped with an electrospray ionization (ESI) source was used and the multiple reaction mode (MRM) was performed. In terms of method validation, good linearity was observed in preassigned validated concentration range for each analyte of interest. Both intra- and inter-batch accuracy was within the range of 87.6-113.8% for all analytes, while intra- and inter-batch precision was below 14.0%. Additionally, both low matrix effects and high recovery were obtained. All analytes remained stable in human plasma at room temperature for 4 h, on wet ice for 8 h, at - 80 °C for 42 d, over three freeze-thaw cycles and under auto-sampler temperature (4 °C) for 48 h post sample preparation. Subsequently, the validated LC-MS/MS method was applied for pharmacokinetic study in healthy Chinese volunteers following an oral dose of 250 mg abiraterone acetate tablet under fasted conditions. Our study for the first time reported the pharmacokinetic parameters of the ABI metabolites in Chinese subjects.
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http://dx.doi.org/10.1016/j.jpba.2022.114826DOI Listing
May 2022

Molecular mechanisms of ARID5B-mediated genetic susceptibility to acute lymphoblastic leukemia.

J Natl Cancer Inst 2022 May 16. Epub 2022 May 16.

Department of Pharmaceutical Sciences, St. Jude Children's Research Hospital, Memphis, TN, USA.

Background: There is growing evidence for the inherited basis of susceptibility to childhood acute lymphoblastic leukemia (ALL). Genome-wide association studies have identified non-coding ALL risk variants at the ARID5B gene locus, but their exact functional effects and the molecular mechanism linking ARID5B to B-ALL leukemogenesis remain largely unknown.

Methods: We performed targeted sequencing of ARID5B in germline DNA of 5,008 children with ALL. Variants were evaluated for association with ALL susceptibility using 3,644 subjects from the UK10K cohort as non-ALL controls, under an additive model. Cis-regulatory elements in ARID5B were systematically identified using CRISPRi enhancer screen in ALL cells. Disruption of transcription factor binding by ARID5B variant was predicted informatically and then confirmed using chromatin immunoprecipitation and co-immunoprecipitation. ARID5B variant association with hematological traits was examined using UK Biobank dataset. All statistical tests are two-sided.

Results: We identified 54 common variants in ARID5B significantly associated with leukemia risk, all of which were non-coding. Six cis-regulatory elements at the ARID5B locus were discovered using CRISPR-based high-throughput enhancer screening. Strikingly, the top ALL risk variant (rs7090445, P=5.57 × 10-45) is located precisely within the strongest enhancer element, which is also distally tethered to the ARID5B promoter. The variant allele disrupts the MEF2C binding motif sequence, resulting in reduced MEF2C affinity and decreased local chromosome accessibility. MEF2C influences ARID5B expression in ALL, likely via a transcription factor complex with RUNX1. Using the UK Biobank dataset (n = 349,861), we showed that rs7090445 was also associated with lymphocyte percentage and count in the general population (P=8.6 × 10-22 and 2.1 × 10-18, respectively).

Conclusion: Our results indicate that ALL risk variants in ARID5B function by modulating cis-regulatory elements at this locus.
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http://dx.doi.org/10.1093/jnci/djac101DOI Listing
May 2022

Specialized Pro-Resolving Mediators Reduce Scarring After Cleft Lip Repair.

Front Immunol 2022 27;13:871200. Epub 2022 Apr 27.

Center for Clinical and Translational Research, Forsyth Institute, Cambridge, MA, United States.

Objective: Residual scarring after cleft lip repair surgery remains a challenge for both surgeons and patients and novel therapeutics are critically needed. The objective of this preclinical experimental study was to evaluate the impact of the methyl-ester of pro-resolving lipid mediator lipoxin A (LXA-ME) on scarring in a novel rabbit model of cleft lip repair.

Methods: A defect of the lip was surgically created and repaired in eight six-week old New Zealand white rabbits to simulate human cleft lip scars. Rabbits were randomly assigned to topical application of PBS (control) or 1 ug of LXA-ME (treatment). 42 days post surgery all animals were euthanized. Photographs of the cleft lip area defect and histologic specimens were evaluated. Multiple scar assessment scales were used to compare scarring.

Results: Animals treated with LXA-ME exhibited lower Visual Scar Assessment scores compared to animals treated with PBS. Treatment with LXA-ME resulted in a significant reduction of inflammatory cell infiltrate and density of collagen fibers. Control animals showed reduced 2D directional variance (orientation) of collagen fibers compared to animals treated with LXA-ME demonstrating thicker and more parallel collagen fibers, consistent with scar tissue.

Conclusions: These data suggest that LXA-ME limits scarring after cleft lip repair and improves wound healing outcomes in rabbits favoring the resolution of inflammation. Further studies are needed to explore the mechanisms that underlie the positive therapeutic impact of LXA-ME on scarring to set the stage for future human clinical trials of LXA-ME for scar prevention or treatment after cleft lip repair.
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http://dx.doi.org/10.3389/fimmu.2022.871200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094441PMC
May 2022

Ferritin Light Chain: A Candidate Autoantigen in Immuno-Related Pancytopenia.

Front Immunol 2022 28;13:851096. Epub 2022 Apr 28.

Department of Hematology, Tianjin Medical University General Hospital, Tianjin, China.

The characteristic feature of immune-related pancytopenia (IRP) is autoantibody-mediated bone marrow (BM) damage and peripheral blood cytopenia. We found that the potential antigen of IRP was Ferritin light chain (FTL) by SEREX (serological analysis of recombinant cDNA expression libraries) in the previous study. In this study, we tried to explore the antigenic epitopes of FTL and verify its antigenicity in IRP. We found the possible FTL epitope: VNLYLQASYTYLSLG by phage random peptide library. Through ELISPOT, it was found that peptide VNLYLQASYTYLSLG can significantly stimulate the production of interleukin-4 and cannot stimulate the production of interferon-γ, which suggested that the peptide can obviously activate Th2 cells. Peptide-major histocompatibility complex tetramer elicited antigen-specific T cell responses. The expression levels of FTL were significantly increased in the patients with untreated IRP (P < 0.05). In conclusion, we found that FTL is the target antigen for some patients with IRP. The peptide of VNLYLQASYTYLSLG is an epitope of the target antigen. The target antigen is abnormally overexpressed on the membrane of BM cells, especially on the surface of CD34+ BM cells of patients with IRP. In addition, it is related to the severity of disease. These results provide a possible new target for the treatment of IRP in the future.
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http://dx.doi.org/10.3389/fimmu.2022.851096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097271PMC
May 2022

Molecular Characteristics of T Cell-Mediated Tumor Killing in Hepatocellular Carcinoma.

Front Immunol 2022 29;13:868480. Epub 2022 Apr 29.

Department of Medical Imaging, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China.

Background: Although checkpoint blockade is a promising approach for the treatment of hepatocellular carcinoma (HCC), subsets of patients expected to show a response have not been established. As T cell-mediated tumor killing (TTK) is the fundamental principle of immune checkpoint inhibitor therapy, we established subtypes based on genes related to the sensitivity to TKK and evaluated their prognostic value for HCC immunotherapies.

Methods: Genes regulating the sensitivity of tumor cells to T cell-mediated killing (referred to as GSTTKs) showing differential expression in HCC and correlations with prognosis were identified by high-throughput screening assays. Unsupervised clustering was applied to classify patients with HCC into subtypes based on the GSTTKs. The tumor microenvironment, metabolic properties, and genetic variation were compared among the subgroups. A scoring algorithm based on the prognostic GSTTKs, referred to as the TCscore, was developed, and its clinical and predictive value for the response to immunotherapy were evaluated.

Results: In total, 18 out of 641 GSTTKs simultaneously showed differential expression in HCC and were correlated with prognosis. Based on the 18 GSTTKs, patients were clustered into two subgroups, which reflected distinct TTK patterns in HCC. Tumor-infiltrating immune cells, immune-related gene expression, glycolipid metabolism, somatic mutations, and signaling pathways differed between the two subgroups. The TCscore effectively distinguished between populations with different responses to chemotherapeutics or immunotherapy and overall survival.

Conclusions: TTK patterns played a nonnegligible role in formation of TME diversity and metabolic complexity. Evaluating the TTK patterns of individual tumor will contribute to enhancing our cognition of TME characterization, reflects differences in the functionality of T cells in HCC and guiding more effective therapy strategies.
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http://dx.doi.org/10.3389/fimmu.2022.868480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100886PMC
May 2022

"Big Data" Approaches for Prevention of the Metabolic Syndrome.

Front Genet 2022 27;13:810152. Epub 2022 Apr 27.

Department of Oncological Neurosurgery, The First Hospital of Jilin University, Changchun, China.

Metabolic syndrome (MetS) is characterized by the concurrence of multiple metabolic disorders resulting in the increased risk of a variety of diseases related to disrupted metabolism homeostasis. The prevalence of MetS has reached a pandemic level worldwide. In recent years, extensive amount of data have been generated throughout the research targeted or related to the condition with techniques including high-throughput screening and artificial intelligence, and with these "big data", the prevention of MetS could be pushed to an earlier stage with different data source, data mining tools and analytic tools at different levels. In this review we briefly summarize the recent advances in the study of "big data" applications in the three-level disease prevention for MetS, and illustrate how these technologies could contribute tobetter preventive strategies.
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http://dx.doi.org/10.3389/fgene.2022.810152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095427PMC
April 2022
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