Publications by authors named "Yang Yu"

4,814 Publications

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Sex-specific association of exposure to bedroom light at night with general and abdominal adiposity in young adults.

Ecotoxicol Environ Saf 2021 Aug 1;223:112561. Epub 2021 Aug 1.

Department of Maternal, Child and Adolescent Health, School of Public Health, Anhui Medical University, Hefei, Anhui, China; Key Laboratory of Population Health Across Life Cycle (Anhui Medical University), Ministry of Education of the People's Republic of China, No 81 Meishan Road, Hefei 230032, Anhui, China; Anhui Provincial Key Laboratory of Population Health and Aristogenics, Anhui Medical University, No 81 Meishan Road, Hefei 230032, Anhui, China. Electronic address:

Experimental animal studies and limited epidemiologic evidence among elder population suggest that exposure to light at night (LAN) may be obesogenic. Nevertheless, little is known about the possible impact of bedroom LAN exposure on subsequent adiposity and the distribution pattern of the accumulated fat, especially in younger population. Here, we estimated longitudinal associations of objectively assessed bedroom LAN exposure with general and abdominal adiposity among young adults. We measured 2-night bedroom LAN exposure using a portable illuminometer in a cohort of young adults (n = 482). Body composition using multifrequency bioelectrical impedance analysis was evaluated at baseline and 1-year follow-up visit. Significant increases in fat mass (2.4 kg, P = 0.015 in male; 1.9 kg, P < 0.001 in female), visceral fat area (10.7 cm, P < 0.001 in male; 5.0 cm, P = 0.01 in female), waist circumference (3.8 cm, P = 0.039 in male; 2.5 cm, P = 0.047 in female) and percentage of body fat (3.6%, P = 0.002 in male; 3.0%, P = 0.001 in female) were observed among individuals with bedroom LAN higher than 5 lx. Compared to the lowest quartile group of bedroom LAN exposure, the highest quartile group was associated with an increase of 0.64 kg/m in BMI (95% CI: 0.18-1.09 kg/m; P = 0.006) and 1.22 kg increase in fat mass (95% CI: 0.10-2.34 kg; P = 0.025) among female participants, and 10.58 cm in visceral fat area (95% CI: 4.85-16.31 cm; P = 0.001) and 2.59 cm in waist circumference (95% CI: 0.37-4.81 cm; P = 0.023) among male participants. In this cohort of Chinese young adults, significant associations were observed between bedroom LAN exposure and adiposity in a sex- and fat depot-specific fashion. Further intervention and longitudinal studies could help elucidate the actual effects and develop sex-specific strategies against lifetime obesity and related metabolic disorders.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112561DOI Listing
August 2021

Vegetable Oil-Based Waterborne Polyurethane as Eco-Binders for Sulfur Cathodes in Lithium-Sulfur Batteries.

Macromol Rapid Commun 2021 Aug 4:e2100342. Epub 2021 Aug 4.

Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China.

Lithium sulfur batteries (LSBs) suffer from well-known fast capacity losses despite their extremely high theoretical capacity and energy density. These losses are caused by dissolution of lithium polysulfide (LiPS) in ether-based electrolytes and have become the main bottleneck to widespread applications of LSBs. Therefore, there is a significant need for electrode materials that have a strong adsorption capacity for LiPS. Herein, a waterborne polyurethane (WPUN) containing sulfamic acid (NH SO H) polymer is designed and synthesized as an aqueous-based, ecofriendly binder by neutralizing sulfamic acid with a tung oil-based polyurethane prepolymer. UV-vis spectroscopy shows that the WPUN strongly immobilizes LiPS and thus is an effective inhibitor of the LiPS. Moreover, the WPUN binder have excellent adhesive and mechanical properties that improves the integrity of sulfur cathodes. The WPUN-based cathodes exhibit a significant improvement in their specific capacity and maintain a capacity of 617 mAh g after 200 cycles at 0.5C. Besides, the LSBs assembled with the WPUN-based cathodes show good rate performance from 0.2C (737 mAh g ) to 4C (586 mAh g ), which is significantly higher than that of LSBs assembled with a commercial polymer binder. The structural design of the presented binder provides a new perspective for obtaining high-performance LSBs. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/marc.202100342DOI Listing
August 2021

Co/N co-doped carbonized wood sponge with 3D porous framework for efficient peroxymonosulfate activation: Performance and internal mechanism.

J Hazard Mater 2021 Jul 24;421:126735. Epub 2021 Jul 24.

School of Chemical Engineering, The University of Adelaide, Adelaide, SA 5005, Australia.

Renewable wood sponge with lamellar structure, compressibility and three-dimensional porous frameworks exhibits excellent functionalization application potential in various fields. Herein, cobalt and nitrogen (Co/N) co-doped carbonized wood sponge (CoNCWS800) was prepared successfully for peroxymonosulfate (PMS) activation to degrade sulfamethoxazole (SMX). The CoNCWS800 material exhibited admirable catalytic activity in PMS activation to oxidize SMX molecules (99.7% within 60 min). Electron paramagnetic resonance (EPR) analysis, quenching tests and electrochemical experiments confirmed the existence of both radical (SO,·OH and O) and non-radical (O and direct charge transfer) pathways during the SMX degradation process. Co species were verified as major contributors for the generation of multiple radicals via activating PMS. Surface defective structure and ketonic CO groups performed the positive linear correlation with reaction kinetic constants, revealing the critical role of the two active sites in PMS activation via non-radical process. This study provides a unique insight in PMS activation mechanism via both radical and non-radical pathways of wood sponge-based functional materials.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126735DOI Listing
July 2021

Particles and corrected particles of LDL and non-HDL are stronger predicters of coronary lesion in postmenopausal women.

BMC Cardiovasc Disord 2021 Aug 2;21(1):369. Epub 2021 Aug 2.

Department of Thoracic and Cardiovascular Surgery, Special Medical Center of Chinese People's Armed Police Force, 220 Chenglin Road, Tianjin, 300162, China.

Background: The optimum lipid indexes, predicting the coronary lesion in postmenopausal women are not clear.

Objective: To evaluate the optimum lipid predicter for coronary lesion in routine and advanced lipid tests.

Method: 300 postmenopausal women were enrolled and assigned into coronary heart disease (CHD) Group (242), and non-CHD Group (58). Routine and advanced lipid indexes were measured with standard laboratory test and nuclear magnetic resonance (NMR) spectroscopy. The correlation and predictivities for CHD of routine and advanced lipid indexes were performed with Logistic regression, Spearman correlation analysis and receiver operating characteristic (ROC).

Results: Age (hazard ratio (HR) 2.58, 95% confidence interval (CI) 1.08-5.86, P = 0.03), apolipoprotein B (ApoB) (HR 1.35, 95% CI 1.15-1.59, P < 0.001), corrected particles of low-density lipoprotein (LDL-p-corr) (HR 1.05, 95% CI 1.03-1.06, P < 0.001) and corrected particles of non-high-density lipoprotein (non-HDL-p-corr) (HR 1.02, 95% CI 1.01-1.03, P < 0.001) were the risk factors of CHD. LDL cholesterol (LDL-C), LDL-p, LDL-p-corr, HDL cholesterol (HDL-C), non-HDL cholesterol (non-HDL-C), non-HDL-p and non-HDL-p-corr were in linear correlation with Gensini score. Advanced lipid indexes LDL-p (area under curve (AUC) = 0.750, P = 0.02), LDL-p-corr (AUC = 0.759, P = 0.02), non-HDL-p (AUC = 0.693, P = 0.03) and non-HDL-p-corr (AUC = 0.699, P = 0.03) were more predictive for CHD than the routine ones (LDL-C and non-HDL-C).

Conclusion: In postmenopausal women, age, ApoB, LDL-p-corr and non-HDL-p-corr were risk factors of CHD. Compared with traditional lipid items, LDL-p, LDL-p-corr, non-HDL-p and non-HDL-p-corr may be better lipid indexes for CHD in postmenopausal women.
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http://dx.doi.org/10.1186/s12872-021-02189-xDOI Listing
August 2021

Genetic diversity and population structure of the rice false smut pathogen in Sichuan-Chongqing region.

Plant Dis 2021 Aug 2. Epub 2021 Aug 2.

Southwest University, 26463, College of Plant Protection, Chongqing, China;

Rice false smut caused by is currently one of the most devastating fungal diseases of rice panicles worldwide. In this study, two novel molecular markers derived from SNP-rich genomic DNA fragments and a previously reported molecular marker were used for analyzing the genetic diversity and population structure of 167 isolates collected from nine areas in Sichuan-Chongqing region, China. A total of 62 haplotypes were identified, and a few haplotypes with high frequency were found and distributed in two to three areas, suggesting gene flow among different geographical populations. All isolates were divided into six genetic groups. The groups Ⅰ and Ⅵ were the largest including 61 and 48 isolates, respectively. The pairwise F values showed significant genetic differentiation among all compared geographical populations. AMOVA showed that intergroup genetic variation accounted for 40.17% of the total genetic variation, while 59.83% of genetic variation came from intragroup. The UPGMA dendrogram and population structure revealed that the genetic composition of isolates collected from ST (Santai), NC (Nanchong), YC (Yongchuan), and WS (Wansheng) dominated by the same genetic subgroup was different from those collected from other areas. In addition, genetic recombination was found in a few isolates. These findings will help to improve the strategies for rice false smut management and resistance breeding, such as evaluating breeding lines with different isolates or haplotypes at different elevations and landforms.
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http://dx.doi.org/10.1094/PDIS-04-21-0750-REDOI Listing
August 2021

Indole Inhibitors of MMP-13 for Arthritic Disorders.

ACS Omega 2021 Jul 19;6(29):18635-18650. Epub 2021 Jul 19.

Boehringer Ingelheim Pharmaceuticals Inc., 900 Ridgebury Road, Ridgefield, Connecticut 06877-0368, United States.

Here, we described the design, by fragment merging and multiparameter optimization, of selective MMP-13 inhibitors that display an appropriate balance of potency and physicochemical properties to qualify as tool compounds suitable for testing. Optimization of potency was guided by structure-based insights, specifically to replace an ester moiety and introduce polar directional hydrogen bonding interactions in the core of the molecule. By introducing polar enthalpic interactions in this series of inhibitors, the overall beneficial physicochemical properties were maintained. These physicochemical properties translated to excellent drug-like properties beyond potency. In a murine model of rheumatoid arthritis, treatment of mice with selective inhibitors of MMP-13 resulted in a statistically significant reduction in the mean arthritic score vs control when dosed over a 14 day period.
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http://dx.doi.org/10.1021/acsomega.1c01320DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319936PMC
July 2021

Ablation of lncRNA attenuates pathological hypertrophy and heart failure.

Theranostics 2021 6;11(16):7995-8007. Epub 2021 Jul 6.

Molecular Cardiology Program, Department of Biomedical Engineering, University of Alabama at Birmingham, School of Medicine and School of Engineering, Birmingham, AL 35294, USA.

The conserved long non-coding RNA (lncRNA) myocardial infarction associate transcript () was identified for its multiple single-nucleotide polymorphisms that are strongly associated with susceptibility to MI, but its role in cardiovascular biology remains elusive. Here we investigated whether regulates cardiac response to pathological hypertrophic stimuli. Both an angiotensin II (Ang II) infusion model and a transverse aortic constriction (TAC) model were used in adult WT and -null knockout (-KO) mice to induce pathological cardiac hypertrophy. Heart structure and function were evaluated by echocardiography and histological assessments. Gene expression in the heart was evaluated by RNA sequencing (RNA-seq), quantitative real-time RT-PCR (qRT-PCR), and Western blotting. Primary WT and -KO mouse cardiomyocytes were isolated and used in Ca transient and contractility measurements. Continuous Ang II infusion for 4 weeks induced concentric hypertrophy in WT mice, but to a lesser extent in -KO mice. Surgical TAC for 6 weeks resulted in decreased systolic function and heart failure in WT mice but not in -KO mice. In both models, -KO mice displayed reduced heart-weight to tibia-length ratio, cardiomyocyte cross-sectional area, cardiomyocyte apoptosis, and cardiac interstitial fibrosis and a better-preserved capillary density, as compared to WT mice. In addition, Ang II treatment led to significantly reduced mRNA and protein expression of the Ca cycling genes Sarcoplasmic/endoplasmic reticulum Ca ATPase 2a (SERCA2a) and ryanodine receptor 2 (RyR2) and a dramatic increase in global RNA splicing events in the left ventricle (LV) of WT mice, and these changes were largely blunted in -KO mice. Consistently, cardiomyocytes isolated from -KO mice demonstrated more efficient Ca cycling and greater contractility. Ablation of attenuates pathological hypertrophy and heart failure, in part, by enhancing cardiomyocyte contractility.
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http://dx.doi.org/10.7150/thno.50990DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315059PMC
July 2021

A light-initiated chemiluminescent assay for the detection of children's milk protein-specific IgE with excellent ability to avoid interference of specific IgG.

J Immunol Methods 2021 Jul 29:113110. Epub 2021 Jul 29.

School of Medical Laboratory, Tianjin Medical University, Tianjin, China.

The detection of allergen-specific IgE is of value for the diagnosis of children's milk allergy. However, its accuracy will interfere with the presence of high levels of specific IgG in the serum of children with milk allergy. To solve this problem, we established a light-initiated chemiluminescent assay (LICA) based on nanomicrospheres, which neutralized the interference of specific IgG by increasing the amount of antigen coated on the microspheres. The ability of this method to resist IgG interference was confirmed by adding extra specific IgG to the serum of allergic patients. Finally, the positive rate of allergen-specific IgE was increased to 85%, which was better than the indirect ELISA (70%), indicating that this method has certain advantages for the detection of specific IgE in children with milk allergy.
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http://dx.doi.org/10.1016/j.jim.2021.113110DOI Listing
July 2021

The effects of high-sensitivity C-reactive protein on the clinical outcomes in obstructive sleep apnea patients undergoing off-pump coronary artery bypass grafting.

BMC Cardiovasc Disord 2021 Jul 31;21(1):366. Epub 2021 Jul 31.

Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, China.

Purpose: To investigate the relationship between obstructive sleep apnea (OSA) severity and high-sensitivity C-reactive protein (Hs-CRP), and their respective impact on the clinical outcomes in patients undergoing off-pump cardiac artery bypass grafting (OPCABG).

Methods: We enrolled consecutive eligible patients listed for elective OPCABG who underwent cardiorespiratory polygraphy before surgery between January 2019 and December 2019 in this prospective observational single-center study. Baseline, intraoperative, and postoperative clinical data were compared between absent-mild and moderate-severe OSA groups. Regression analysis investigated the relationship between Hs-CRP level and severity of OSA, and further assessed the factors influencing postoperative atrial fibrillation, duration of hospitalization, and hospital cost.

Results: Patients with moderate-severe OSA accounted for 42.3% (52/123) of the cohort. Partial pressure of carbon dioxide (PCO), Hs-CRP, apnea hypopnea index (AHI), mean apnea time, maximum apnea time, and oxygen desaturation index ODI ≥ 3% were significantly higher in the moderate-severe OSA group than in the absent-mild OSA group. Left ventricle ejection fraction (LVEF), lowest arterial oxygen saturation (SaO), and mean SaO were significantly lower in the moderate-severe OSA group. Moderate-severe OSA was associated with elevated Hs-CRP level (OR = 2.356, 95% CI 1.101-5.041, P = 0.027). Hs-CRP was an independent risk factor for post-CABG atrial fibrillation (POAF) (OR = 1.212, P = 0.01). Hs-CRP level independently correlated with duration of hospitalization (B = 0.456, P = 0.001) and hospital cost (B = 1.111, P = 0.044).

Conclusion: Hs-CRP level was closely related to OSA severity and have potential utility in predicting POAF, duration of hospitalization, and hospital costs in patients undergoing OPCABG.
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http://dx.doi.org/10.1186/s12872-021-02168-2DOI Listing
July 2021

Acesulfame aerobic biodegradation by enriched consortia and Chelatococcus spp.: Kinetics, transformation products, and genomic characterization.

Water Res 2021 Jul 23;202:117454. Epub 2021 Jul 23.

Environmental Microbiome Engineering and Biotechnology Lab, Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China. Electronic address:

The artificial sweetener Acesulfame (ACE) has been frequently detected in wastewater treatment plants (WWTPs) and is regarded as an emerging pollutant due to its low biodegradability. However, recent observations of ACE biodegradation in WWTPs have stimulated interest in the ACE-degrading bacteria and mineralization pathways. In this study, next-generation sequencing methods, Illumina and Nanopore sequencing, were combined to explore the ACE-degrading communities enriched from the activated sludge of six municipal wastewater treatment plants. Metagenomic investigations indicated that all enrichments were similarly dominated by the phyla Proteobacteria and Planctomycetes. Notably, at the species level, four metagenome-assembled genomes (MAGs) were shared by six enriched communities with considerable abundances, indicating that they may be responsible for ACE biodegradation in the enrichments. Besides, two ACE-degrading pure strains, affiliated to the genus Chelatococcus, were isolated from the enrichment. The genomic analysis showed that these two isolates were the new species that were genetically distinct from their relatives. Two type strains, Chelatococcus asaccharovorans DSM 6462 and Chelatococcus composti DSM 101465, could not degrade ACE, implying that the ACE-degrading capability was not shared among the different species in the genus Chelatococcus. The results of the degradation experiment showed that the two isolates could use ACE as the sole carbon source and mineralize ~90% of the total organic carbon. Three biotransformation products (TP96, TP180B, and TP182B) were demonstrated by UPLC-QTOF-MS. The results of this study provide valuable insights into ACE biodegradation and its biotransformation products.
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http://dx.doi.org/10.1016/j.watres.2021.117454DOI Listing
July 2021

SERS-ELISA determination of human carboxylesterase 1 using metal-organic framework doped with gold nanoparticles as SERS substrate.

Mikrochim Acta 2021 Jul 30;188(8):280. Epub 2021 Jul 30.

Guangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, No.268 Donghuan Road, Chengzhong District, Liuzhou City, 545006, Guangxi Zhuang Autonomous Region, China.

By in situ synthesis of gold nanoparticles (AuNPs) within the acid-etched (AE) MIL-101 (Cr) framework, AE-MIL-101 (Cr) nanocomposites embedded with AuNPs (AuNP/AE-MIL-101 (Cr)) were prepared as surface-enhanced Raman scattering (SERS) substrate. AuNPs are uniformly distributed and stabilized inside the metal-organic framework (MOF), thus forming more SERS hotspots. The SERS performance of AuNP/AE-MIL-101 (Cr) was evaluated using 4-mercaptophenylboronic acid (4-MPBA), 4-mercaptobenzoic acid (4-MBA), benzidine, and rhodamine 6G (R6G). The SERS substrate displays satisfying stability with very low background signal. When benzidine is used as the Raman reporter, the limit of detection (LOD) can reach 6.7 × 10 mol·L, and the relative standard deviation (RSD) of the intra- and inter-batch repetitive tests is less than 5.2%. On this basis, we developed a method for the detection of human carboxylesterase 1 (hCE 1) in human serum using AuNP/AE-MIL-101 (Cr) nanocomposite as SERS substrate and enzyme-linked immunosorbent assay (ELISA) colorimetric substrate as SERS marker. This method was used to determine hCE 1 in clinical serum samples without complicated sample pretreatment, and the detection results were consistent with the data determined by ELISA. In the concentration range 0.1-120 ng·mL, the SERS signal intensity of benzidine at 1609 cm gradually decreases with the increase of hCE 1 concentration (R = 0.9948). The average recoveries of hCE 1 in human serum are in the range 84 to 108%, with RSDs lower than 7.7%. By using AuNP/acid etching-MIL-101(Cr) metal organic framework (MOF) as SERS substrate and enzyme-linked immunosorbent assay (ELISA) colorimetric substrate as the SERS marker, a rapid and sensitive method for the determination of human carboxylesterase 1 (hCE1) in human serum samples has been developed.
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http://dx.doi.org/10.1007/s00604-021-04928-5DOI Listing
July 2021

Characteristic of HBV nucleic acid amplification testing yields from blood donors in China.

BMC Infect Dis 2021 Jul 30;21(1):714. Epub 2021 Jul 30.

Blood Center of Zhejiang Province, Hangzhou, 310052, Zhejiang, People's Republic of China.

Background: Nucleic acid amplification testing (NAT) for blood screening has been previously performed in some countries to determine NAT yields. The current study sought to explore the non-discriminating reactive NAT yields using individual-NAT (ID-NAT) and characteristics of HBV NAT yields through a 10-year retrospective analysis in Zhejiang, China.

Methods: Blood donations were analyzed using individual-NAT mode by the transcription-mediated amplification (TMA) method. Supplementary HBV serological tests were performed using chemiluminescent immunoassay, and HBV viral load assay was performed by real-time polymerase chain reaction. Follow-up studies were performed in partial donors with low HBV viral loads.

Results: Non-discriminating reactive NAT yields and HBV NAT yields varied in different years. The yields ranged from 853.73 per million to 2018.68 per million and 624.60 per million to 1669.50 per million, respectively. In the 476 NAT yields, 19 were probable window periods (WP), 33 probable occult hepatitis B virus infections (OBIs), 409 were confirmed OBIs and 15 were chronic HBV infections. ID-NAT results were categorized in four groups, and the findings showed that the levels of HBV DNA viral loads were different in the four different groups (χ = 275.02, p < 0.01). HBV viral load distribution was significantly different between anti-HBs positive and anti-HBc positive samples (χ = 49.429, p < 0.01). Notably, only 42.03% donors were NAT repeated positive in the 138 repeat donors' follow up tests.

Conclusion: NAT screening of blood donations can reduce the risk of transfusion-transmitted HBV infections. Positive proportions of anti-HBs and anti-HBc are correlated with the HBV viral load level. However, low level of viral load donors pose risks in HBV NAT assays, and show fluctuating state for HBV viral load and leads to non-repeated NAT results during follow up studies.
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http://dx.doi.org/10.1186/s12879-021-06468-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325190PMC
July 2021

Alkaloid derivative ION-31a inhibits breast cancer metastasis and angiogenesis by targeting HSP90α.

Bioorg Chem 2021 Jul 22;115:105201. Epub 2021 Jul 22.

School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China; Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, PR China. Electronic address:

Breast cancer has become the number one killer of women. In our previous study, an active compound, ION-31a, with potential anti-metastasis activity against breast cancer was identified through the synthesis of ionone alkaloid derivatives. In the present study, we aimed to identify the therapeutic target of ION-31a. We used a fluorescence tag labeled probe, molecular docking simulation, and surface plasmon resonance (SPR) analysis to identify the target of ION-31a. The main target of ION-31a was identified as heat shock protein 90 (HSP90). Thus, ION-31a is a novel HSP90 inhibiter that could suppress the metastasis of breast cancer and angiogenesis significantly in vitro and in vivo. ION-31a acts via inhibiting the HSP90/hypoxia inducible factor 1 alpha (HIF-1α)/vascular endothelial growth factor (VEGF)/VEGF receptor 2 (VEGFR2) pathway and downregulating downstream signal pathways, including protein kinase B (AKT)/mammalian target of rapamycin (mTOR), AKT2/protein kinase C epsilon (PKCζ), extracellular regulated kinase 1/2 (ERK1/2), focal adhesion kinase (FAK), and mitogen-activated protein kinase 14 (p38MAPK) pathways. ION-31a affects multiple effectors implicated in tumor metastasis and has the potential to be developed as an anti-metastatic agent to treat patients with breast cancer.
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http://dx.doi.org/10.1016/j.bioorg.2021.105201DOI Listing
July 2021

Water and the Cation-π Interaction.

J Am Chem Soc 2021 Jul 30. Epub 2021 Jul 30.

Center for Supramolecular Chemistry & Catalysis and Department of Chemistry, College of Science, Shanghai University, 99 Shang-Da Road, Shanghai 200444, China.

The cation-π interaction and the hydrophobic effect are important intermolecular forces in chemistry and play major roles in controlling recognition in biological systems. We compared their relative contributions to the binding of molecular "dumbbell" guests in synthetic container hosts in water. The guests offered direct, intramolecular competition between trimethylammonium groups, -N(CH), and -butyl groups, -C(CH), for the internal surfaces (aromatic panels) of the containers. In contrast with previous studies, the container molecules consistently preferred binding to the uncharged -butyl groups, regardless of the presence of anionic, cationic, or zwitterionic groups on the container peripheries. This preference is determined by solvation of the polar trimethylammonium group in water, which outcompetes the attraction between the positive charge and the π-surfaces in the container. The synthetic container complexes provide a direct measure of the relative strengths of cation-π interactions and desolvation in water. Interactions with the uncharged -butyl group are more than 12 kJ mol more favorable than the cation-π interactions with the trimethylammonium group in these cavitand complexes.
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http://dx.doi.org/10.1021/jacs.1c06510DOI Listing
July 2021

Arteannoides U-Z: Six undescribed sesquiterpenoids with anti-inflammatory activities from the aerial parts of Artemisia annua (Qinghao).

Fitoterapia 2021 Jul 26:105002. Epub 2021 Jul 26.

Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drug Research, Jinan University, Guangzhou 510632, China. Electronic address:

Four previously unreported sesquiterpenoid diasteromers, arteannoides U-X (1-4), together with one new norsesquiterpenoid 5 (arteannoide Y) and one undescribed rearranged cadinene sesquiterpenoid 6 (arteannoide Z) were obtained from the dried aerial parts of Artemisia annua (Qinghao). Notably, arteannoides U-X (1-4) are four stereoisomers that possess the same molecules and the same planar connectivity, but differ from each other in configuration at a certain stereocenter. Their accurate structures were unambiguously identified and distinguished by extensive spectroscopic analyses, NMR calculations with DP4+ analysis, electronic circular dichroism (ECD) calculations and X-ray diffraction analyses. Compounds 1, 3, and 4 showed inhibitory activities against the production of inflammatory cytokines (PGE, NO, IL-6 and TNF-α) in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages.
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http://dx.doi.org/10.1016/j.fitote.2021.105002DOI Listing
July 2021

Artificial intelligence in small intestinal diseases: Application and prospects.

World J Gastroenterol 2021 Jul;27(25):3734-3747

Trauma Research Center, The Fourth Medical Center and Medical Innovation Research Division of the Chinese People's Liberation Army General Hospital, Beijing 100048, China.

The small intestine is located in the middle of the gastrointestinal tract, so small intestinal diseases are more difficult to diagnose than other gastrointestinal diseases. However, with the extensive application of artificial intelligence in the field of small intestinal diseases, with its efficient learning capacities and computational power, artificial intelligence plays an important role in the auxiliary diagnosis and prognosis prediction based on the capsule endoscopy and other examination methods, which improves the accuracy of diagnosis and prediction and reduces the workload of doctors. In this review, a comprehensive retrieval was performed on articles published up to October 2020 from PubMed and other databases. Thereby the application status of artificial intelligence in small intestinal diseases was systematically introduced, and the challenges and prospects in this field were also analyzed.
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http://dx.doi.org/10.3748/wjg.v27.i25.3734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291013PMC
July 2021

Prediction of lymphovascular space invasion using a combination of tenascin-C, cox-2, and PET/CT radiomics in patients with early-stage cervical squamous cell carcinoma.

BMC Cancer 2021 Jul 28;21(1):866. Epub 2021 Jul 28.

Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China.

Background: Lymphovascular space invasion is an independent prognostic factor in early-stage cervical cancer. However, there is a lack of non-invasive methods to detect lymphovascular space invasion. Some researchers found that Tenascin-C and Cyclooxygenase-2 was correlated with lymphovascular space invasion. Radiomics has been studied as an emerging tool for distinguishing tumor pathology stage, evaluating treatment response, and predicting prognosis. This study aimed to establish a machine learning model that combines radiomics based on PET imaging with tenascin-C (TNC) and cyclooxygenase-2 (COX-2) for predicting lymphovascular space invasion (LVSI) in patients with early-stage cervical cancer.

Methods: One hundred and twelve patients with early-stage cervical squamous cell carcinoma who underwent PET/CT examination were retrospectively analyzed. Four hundred one radiomics features based on PET/CT images were extracted and integrated into radiomics score (Rad-score). Immunohistochemical analysis was performed to evaluate TNC and COX-2 expression. Mann-Whitney U test was used to distinguish differences in the Rad-score, TNC, and COX-2 between LVSI and non-LVSI groups. The correlations of characteristics were tested by Spearman analysis. Machine learning models including radiomics model, protein model and combined model were established by logistic regression algorithm and evaluated by ROC curve. Pairwise comparisons of ROC curves were tested by DeLong test.

Results: The Rad-score of patients with LVSI was significantly higher than those without. A significant correlation was shown between LVSI and Rad-score (r = 0.631, p < 0.001). TNC was correlated to both the Rad-score (r = 0.244, p = 0.024) and COX-2 (r = 0.227, p = 0.036). The radiomics model had the best predictive performance among all models in training and external dataset (AUCs: 0.914, 0.806, respectively, p < 0.001). However, in testing dataset, the combined model had better efficiency for predicting LVSI than other models (AUCs: 0.801 vs. 0.756 and 0.801 vs. 0.631, respectively).

Conclusion: The machine learning model of the combination of PET radiomics with COX-2 and TNC provides a new tool for detecting LVSI in patients with early-stage cervical cancer. In the future, multicentric studies on larger sample of patients will be used to test the model.

Trial Registration: This is a retrospective study and there is no experimental intervention on human participants. The Ethics Committee has confirmed that retrospectively registered is not required.
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http://dx.doi.org/10.1186/s12885-021-08596-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317359PMC
July 2021

Overexpression of the rice ORANGE gene OsOR negatively regulates carotenoid accumulation, leads to higher tiller numbers and decreases stress tolerance in Nipponbare rice.

Plant Sci 2021 Sep 7;310:110962. Epub 2021 Jun 7.

Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Harbin, China; College of Advanced Agricultural Science, University of Chinese Academy of Sciences, Beijing, China. Electronic address:

The ORANGE (OR) gene has been reported to regulate chromoplast differentiation and enhance carotenoid biosynthesis in many dicotyledonous plants. However, the function of the OR gene in monocotyledons, especially rice, is poorly known. Here, the OR gene from rice, OsOR, was isolated and characterized by generating overexpressing and genome editing mutant lines. The OsOR-overexpressing plants exhibited pleiotropic phenotypes, such as alternating transverse green and white sectors on leaves at the early tillering stage, that were due to changes in thylakoid development and reduced carotenoid content. In addition, the number of tillers significantly increased in OsOR-overexpressing plants but decreased in osor mutant lines, a result similar to that previously reported for the carotenoid isomerase mutant mit3. The expression of the DWARF3 and DWARF53 genes that are involved in the strigolactone signalling pathway were similarly downregulated in OsOR-overexpressing plants but upregulated in osor mutants. Moreover, the OsOR-overexpressing plants exhibited greater sensitivity to salt and cold stress, and had lower total chlorophyll and higher MDA contents. All results suggest that the OsOR gene plays an important role not only in carotenoid accumulation but also in tiller number regulation and in responses to environmental stress in rice.
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http://dx.doi.org/10.1016/j.plantsci.2021.110962DOI Listing
September 2021

Emission characteristics of water-soluble ions in PM released by forest fuel combustion in Great Xing'an Mountains, Inner Mongolia, China.

Ying Yong Sheng Tai Xue Bao 2021 Jul;32(7):2316-2324

College of Forestry, Inner Mongolia Agricultural University, Hohhot 010019, China.

Understanding the emission factors of fine particulate matter (PM) released by forest fuel combustion is important for revealing the impacts of forest fire on atmosphere and ecosystem. Water-soluble ions are important components of fine particulate matter, with great significance to the formation of particulate matter. A self-designed biomass combustion system was used to simulate the combustion of three components (trunks, branches, barks) and their surface dead fuel (litter, semi-humus, humus) of five tree species (, , , , ) and branches of three shrub species (, , ) in Great Xing'an Mountains in Inner Mongolia. The water-soluble ion emission factors (Na, NH, K, Mg, Ca, F, Cl, NO, NO, SO) in PM under two combustion conditions (smoldering and flaming) were measured by ISC1100 ion chromatograph. The results showed that for the water-soluble ion detected in PM from combustion of all types of materials, K, Cl and Na were the main components in smoldering, while K, Cl and SO were the main components in flaming. There was significant difference in the total amount of water-soluble ions in PM from the same type of material under different combustion conditions. During the smoldering period, the emission factor of water-soluble inorganic ions in PM of shrub branches was higher than that of flaming. The cation to anion ratio in PM was 1.26 for all trees, 1.12 for surface dead fuel of trees, and 2.0 for branch of shrub, indicating that the particulate matter was alkaline. Forest fires in Great Xing'an Mountains could not result in ecosystem acidification by releasing water-soluble ions.
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http://dx.doi.org/10.13287/j.1001-9332.202107.003DOI Listing
July 2021

Recognition of hydrophilic molecules in deep cavitand hosts with water-mediated hydrogen bonds.

Chem Commun (Camb) 2021 Jul 27. Epub 2021 Jul 27.

Center for Supramolecular Chemistry & Catalysis and Department of Chemistry, College of Science, Shanghai University, 99 Shang-Da Road, Shanghai 200444, China.

We describe new container host molecules - deep cavitands with benzimidazole walls and ionic feet - to recognize highly hydrophilic guest molecules in water. The aromatic surfaces of the cavity recognize hydrophobic portions of the guest while bound water molecules mediate hydrogen bonding in the complex. Spectroscopic (NMR) evidence indicates slow in/out exchange on the chemical shift timescale and thermodynamic (ITC) methods show large association constants (Ka up to 6 × 104 M-1) for complexation of small, water-soluble molecules such as THF and dioxane. Quantum chemical calculations are employed to optimize the host-guest geometries and elucidate the hydrogen bonding patterns responsible for the binding.
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http://dx.doi.org/10.1039/d1cc02505hDOI Listing
July 2021

Incidence, demographics, and survival of patients with primary pituitary tumors: a SEER database study in 2004-2016.

Sci Rep 2021 Jul 26;11(1):15155. Epub 2021 Jul 26.

Department of Neurosurgery, West China Hospital of Sichuan University, No. 37, Guoxue Alley, Chengdu, 610041, Sichuan Province, People's Republic of China.

Comprehensive investigations on the incidence and prognosis of pituitary tumors are still lacking. The present study aims to summarize the incidence, demographics, and survival outcome of pituitary adenoma on a population-based level. This study includes all pituitary adenomas reported in the Surveillance, Epidemiology, and End Results (SEER) database from 2004 to 2016 in the United States. Extensive clinical and demographic characteristics were extracted and submitted to group comparisons. The standardized incidence rate was calculated and stratified by year at diagnosis, age/sex and age/treatment groups. The Kaplan-Meier analysis and multivariable regressions were performed to identify the factors associated with overall survival. A total of 47,180 pituitary tumors were identified, including 47,030 typical adenomas, 111 uncertain behavior pituitary adenomas, and 39 pituitary carcinomas. The overall standardized incidence rate was 4.8 cases per 100,000 person-years and the annual incidence rate continually trended upwards, with a peak seen in 2015. We noticed a bimodal age-related distribution in females and a unimodal distribution in males. In the multivariate regression analysis, the factors associated with prolonged survival included typical adenoma, younger age, and smaller tumor size. Whereas, black and male patients had worse overall survival. Our study provides a reliable estimate on the incidence of pituitary adenoma and confirms that the annual standardized incidence rate is increasing. Pituitary adenomas have a satisfactory long-term prognosis and age, tumor size, and tumor subtypes are related to overall survival. Though statistically significant, our inferential findings should be constrained within the limitations of SEER database.
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http://dx.doi.org/10.1038/s41598-021-94658-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313564PMC
July 2021

Development of improved SRC-3 inhibitors as breast cancer therapeutic agents.

Endocr Relat Cancer 2021 Jul 1. Epub 2021 Jul 1.

D Lonard, Dept. of Molecular and Cellular Biology, Baylor College of Medicine, Houston, United States.

Steroid receptor coactivators (SRCs) possess specific and distinct oncogenic roles in the initiation of cancer and in cancer progression to a more aggressive disease. These coactivators interact with nuclear receptors and other transcription factors to boost transcription of multiple genes which potentiate cancer cell proliferation, migration, invasion, tumor angiogenesis and epithelial mesenchymal transition (EMT). Targeting SRCs using small molecule inhibitors (SMIs) is a promising approach to control cancer progression and metastasis. By high throughput screening analysis, we recently identified SI-2 as a potent SRC SMI. To develop therapeutic agents, SI-10 and SI-12, the SI-2 analogs, are synthesized that incorporate the addition of fluorine atoms to the SI-2 chemical structure. As a result, these analogs exhibit a significantly prolonged plasma half-life, minimal toxicity and improved hERG activity. Biological functional analysis showed that SI-10 and SI-12 treatment (5-50 nM) can significantly inhibit viability, migration and invasion of breast cancer cells in vitro and repress the growth of breast cancer PDX organoids. Treatment of mice with 10 mg/kg/day of either SI-10 or SI-12 was sufficient to repress growth of xenograft tumors derived from MDA-MB-231 and LM2 cells. Furthermore, in spontaneous and experimental metastasis mouse models developed from MDA-MB-231 and LM2 cells respectively, SI-10 and SI-12 effectively inhibited progression of breast cancer lung metastasis. These results demonstrate that SI-10/SI-12 are promising therapeutic agents and are specifically effective in blocking tumor metastasis, a key point in tumor progression to a more lethal state that results in patient mortality in the majority of cases.
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http://dx.doi.org/10.1530/ERC-20-0402DOI Listing
July 2021

Efficacy and safety of Zi Gui Nv Zhen capsules used in TCM for fertility preservation in patients with diminished ovarian reserve.

Gynecol Endocrinol 2021 Jul 26:1-5. Epub 2021 Jul 26.

Department of Gynecological Endocrinology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.

Objective: To evaluate for the first time whether Zi Gui Nv Zhen capsules (ZGNZC), until now used in traditional Chinese medicine (TCM) for menopausal complaints, can increase the fertility of Chinese women with diminished ovarian reserve (DOR).

Methods: Prospective, randomized, open-labeled 3-monthly study; 109 DOR patients (aged 20-40 years) receiving either ZGNZC (experimental group,  = 75) or not (control group,  = 34). Main outcomes: markers for ovarian function, thickness/type of the endometrium during ovulation, and pregnancy rate. Between-group analysis (A) comparing experimental vs. control group and within-group analysis (B) comparing data at baseline and after study in each of both groups.

Results: (A) Between-group-analysis: patients with ZGNZC had a higher endometrium thickness (0.75 vs. 0.62; <.05) and higher anti-Müllerian hormone (AMH, 0.50 vs. 0.40; <.05) than control group. Pregnancy rates were higher in the experimental than the control group (26.7% vs. 14.7%; n.s.). (B) Within-group-analysis: ZGNZC decreased levels of follicle-stimulating hormone (FSH, 11.42 vs. 8.69), increased estradiol-levels (E, 56.09 vs. 73.36), and type A endometrium rates (5.3% vs. 39.7%) (all < .05) and increased antral follicle count (AFC, 2 vs. 3). All hepato-renal biomarkers remained within the norm. The tolerability was good. There were no adverse events.

Conclusions: In women with DOR who wish to conceive, three months' application of ZGNZC can improve ovarian function and oocyte quality by adjusting the neuroendocrine system, can improve endometrial properties and proliferation, necessary for a healthy pregnancy, and increased the clinical pregnancy rate in our prospective randomized observational study.
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http://dx.doi.org/10.1080/09513590.2021.1957095DOI Listing
July 2021

[The safety of TiRobot-guided percutaneous transpedicular screw implantation].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Jul;35(7):813-817

Department of Orthopedics, Sichuan Academy of Medical Science, People's Hospital of Sichuan Province, Chengdu Sichuan, 610072, P.R.China.

Objective: To evaluate the safety of TiRobot-guided percutaneous transpedicular screw implantation.

Methods: The medical records of 158 patients with thoracolumbar fractures and lumbar degenerative diseases who underwent percutaneous transpedicular screw implantation were retrospectively analyzed between January 2018 and December 2020. The patients were divided into trial group (TiRobot-guided screw implantation, 86 cases) and control group (fluoroscopy-guided screw implantation, 72 cases). There was no significant difference in gender, age, pathology, lesion segment, and the average number of screw implantation per case ( >0.05). The operation time, fluoroscopic dose, fluoroscopic time, and fluoroscopic frequency were compared between the two groups. One day postoperatively, the convergence angle was measured and the penetration of the pedicle cortex was evaluated according to Gertzbein-Robbins classification standard.

Results: The operation time, fluoroscopic dose, fluoroscopic time, and fluoroscopic frequency of the trial group were significantly lesser than those of control group ( <0.05). One day postoperatively, the convergence angle of trial group was (21.10±4.08)°, which was significantly larger than control group (19.17±3.48)° ( =6.810, =0.000). According to the Gertzbein-Robbins classification standard, 446 pedicle screws were implanted in trial group, trajectories were grade A in 377 screws, grade B in 46 screws, grade C in 23 screws, and the accuracy of screw implantation was 94.8%; 380 pedicle screws were implanted in control group, trajectories were grade A in 283 screws, grade B in 45 screws, grade C in 44 screws, grade D in 6 screws, grade E in 2 screws, and the accuracy of screw implantation was 86.3%. There was significant difference in the accuracy of screw implantation between the two groups ( =25.950, =0.000).

Conclusion: Compared with traditional percutaneous transpedicular screw implantation, TiRobot-guided percutaneous transpedicular screw implantation can improve the accuracy of screw implantation, reduce radiation exposure, and improve surgical safety, which has a good application prospect.
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http://dx.doi.org/10.7507/1002-1892.202103072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311213PMC
July 2021

Obstructive Sleep Apnea Increases the Risk of Perioperative Myocardial Infarction Following Off-Pump Coronary Artery Bypass Grafting.

Front Cardiovasc Med 2021 8;8:689795. Epub 2021 Jul 8.

Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

The impact of obstructive sleep apnea (OSA) on perioperative myocardial infarction (PMI) following coronary artery bypass grafting (CABG) remains unclear. Off-pump CABG (OPCABG) has become a common practice for CABG in China. The present study investigated mainly the correlation between OSA and PMI following OPCABG. In this prospective observational single-center study, consecutive eligible patients listed for elective OPCABG underwent cardiorespiratory polygraphy before surgery between January 2019 and June 2020. OSA was defined as an apnea-hypopnea index (AHI) ≥15 events/h. The primary end point was perioperative myocardial infarction (PMI) following OPCABG (type 5 MI). Patients with OSA accounted for 42.2% (62/147) of the cohort. Twenty-four patients (16.3%) met the protocol criteria for PMI: 17 (27.4%) in the OSA group and 7 (8.2%) in the non-OSA group ( = 0.002). Multivariate logistic regression analysis revealed that AHI (OR = 1.115, 95% CI 1.066 to 1.166, < 0.001), high-sensitivity c-reactive protein (hs-CRP) (OR = 1.080, 95% CI 1.025 to 1.138, = 0.004), and SYNTAX score (OR = 1.098, 95% CI 1.056 to 1.141, < 0.001) were associated with PMI incidence. Furthermore, ROC analysis revealed that the AHI (AUC = 0.766, 95% CI 0.689 to 0.832, < 0.001) and SYNTAX score (AUC = 0.789, 95% CI 0.715 to 0.852, < 0.001) had predictive value for PMI. In addition, multiple linear regression analysis showed that the AHI was an independent influencing factor of hs-CRP (B = 0.176, 95% CI 0.090 to 0.263, < 0.001) and the SYNTAX score (B = 0.553, 95% CI 0.397 to 0.709, < 0.001). OSA was independently associated with a higher incidence of PMI following OPCABG, and the formation of severe coronary atherosclerotic lesions aggravated by an enhanced inflammatory response might be the potential mechanism. The efficacy of CPAP treatment for improving prognosis after CABG remains to be further investigated.
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http://dx.doi.org/10.3389/fcvm.2021.689795DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296635PMC
July 2021

The diagnostic value of 3.0T MRI in cesarean scar pregnancy.

Am J Transl Res 2021 15;13(6):6229-6235. Epub 2021 Jun 15.

Department of Radiology, Women's and Children's Health Care Hospital of Linyi Linyi 276016, Shandong Province, China.

Objective: The purpose was to evaluate the diagnostic value of 3.0T MRI in cesarean scar pregnancy (CSP).

Methods: 56 patients with suspected CSP treated in our hospital from August 2018 to July 2020 were recruited as the study cohort and diagnosed using ultrasound and 3.0T MRI. With the pathological examination results as the gold standard and the ultrasound examination results as a comparison, the diagnostic value of 3.0T MRI was evaluated according to the diagnostic accuracy, sensitivity, specificity, and positive and negative misdiagnosis rates, etc. Results: The pathological examination showed that 33 patients were positive for CSP but the other 23 were negative. The accuracy, sensitivity, and specificity of 3.0T MRI in the CSP diagnoses were significantly higher than the accuracy, sensitivity, and specificity of the ultrasound diagnoses (P<0.05), and the positive and negative misdiagnosis rates were significantly lower than the misdiagnosis rates of the ultrasound diagnosis (P<0.05). Moreover, the images from two patients showed that 3.0T MRI can provide clear images of the patients' lesion locations.

Conclusion: Compared with ultrasound diagnoses, 3.0TMRI has significant advantages in diagnosing CSP and has a high clinical value.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290699PMC
June 2021

LncRNA LINC00473 promoted colorectal cancer cell proliferation and invasion by targeting miR-195 expression.

Am J Transl Res 2021 15;13(6):6066-6075. Epub 2021 Jun 15.

Department of General Surgery, Rizhao People's Hospital Rizhao 276800, Shandong, China.

Long noncoding RNAs (lncRNAs) have been shown to play crucial roles in cancer development. However, the role of LINC00473 in colorectal cancer has not been explored. In our study, we showed that LINC00473 expression was upregulated in colorectal cancer samples compared to nontumor samples. The expression of LINC00473 in colorectal cancer tissues from patients with distant metastasis was higher than that from cases without distant metastasis. The higher expression level of LINC00473 was positively correlated with advanced clinical stage. The elevated expression of LINC00473 accelerated colorectal cancer cell proliferation, cell cycle progression and invasion. Moreover, overexpression of LINC00473 induced epithelial to mesenchymal (EMT) progression in HT29 and SW480 cells. Ectopic expression of LINC00473 suppressed miR-195 expression in colorectal cancer cells. miR-195 expression was downregulated in colorectal cancer samples compared with nontumor samples. The expression of miR-195 in colorectal cancer tissues from patients with distant metastasis was lower than that from cases without distant metastasis. The lower expression level of miR-195 was positively correlated with advanced clinical stage. In addition, we showed that the expression of miR-195 was negatively correlated with the LINC00473 expression level in colorectal cancer tissues. LINC00473 accelerated colorectal cancer cell proliferation and cell cycle progression and regulated EMT progression by regulating miR-195 expression. These data suggested that LINC00473 induced cell proliferation, cell cycle progression and EMT progression by acting as a ceRNA for miR-195 in colorectal cancer.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290708PMC
June 2021

The characteristics and risk factors of human papillomavirus infection: an outpatient population-based study in Changsha, Hunan.

Sci Rep 2021 Jul 23;11(1):15128. Epub 2021 Jul 23.

Department of Obstetrics and Gynecology, The Third Xiangya Hospital of Central South University, Changsha, 410013, China.

This cross-sectional study investigated the characteristics of cervical HPV infection in Changsha area and explored the influence of Candida vaginitis on this infection. From 11 August 2017 to 11 September 2018, 12,628 outpatient participants ranged from 19 to 84 years old were enrolled and analyzed. HPV DNA was amplified and tested by HPV GenoArray Test Kit. The vaginal ecology was detected by microscopic and biochemistry examinations. The diagnosis of Candida vaginitis was based on microscopic examination (spores, and/or hypha) and biochemical testing (galactosidase) for vaginal discharge by experts. Statistical analyses were performed using SAS 9.4. Continuous and categorical variables were analyzed by t-tests and by Chi-square tests, respectively. HPV infection risk factors were analyzed using multivariate logistic regression. Of the total number of participants, 1753 were infected with HPV (13.88%). Females aged ≥ 40 to < 50 years constituted the largest population of HPV-infected females (31.26%). The top 5 HPV subtypes affecting this population of 1753 infected females were the following: HPV-52 (28.01%), HPV-58 (14.83%), CP8304 (11.47%), HPV-53 (10.84%), and HPV-39 (9.64%). Age (OR 1.01; 95% CI 1-1.01; P < 0.05) and alcohol consumption (OR 1.30; 95% CI 1.09-1.56; P < 0.01) were found to be risk factors for HPV infection. However, the presence of Candida in the vaginal flora was found to be a protective factor against HPV infection (OR 0.62; 95% CI 0.48-0.8; P < 0.001). Comparing with our previous study of 2016, we conclude that the subtype distribution of HPV infection is relatively constant in Changsha. Our data suggest a negative correlation between vaginal Candida and HPV, however, more radical HPV management is required in this area for perimenopausal women and those who regularly consume alcohol.
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http://dx.doi.org/10.1038/s41598-021-94635-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8302608PMC
July 2021

Efficacy of lenvatinib plus pembrolizumab in hepatocellular carcinoma with metachronous lung metastasis: a report of two cases.

Tumori 2021 Jul 23:3008916211033353. Epub 2021 Jul 23.

Department of Abdominal Oncology, Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China.

Introduction: Immune checkpoint inhibitors (ICIs) and a combination of ICIs with targeted antiangiogenic agents are effective in treatment of advanced hepatocellular carcinoma (HCC). Studies have not explored effective biomarkers for prediction of HCC sensitivity to immunotherapy.

Case Description: The current study explored two consecutive patients with HCC with metachronous lung metastasis who were treated with lenvatinib and pembrolizumab as first-line treatment and third-line treatment, respectively. The two cases showed significant tumor shrinkage and long progression-free survival (>19 months and 12 months, respectively).

Conclusions: The findings of the current study and recently published data indicate lung metastasis as a potential clinical therapeutic indicator for efficacy of immunotherapy against HCC.
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http://dx.doi.org/10.1177/03008916211033353DOI Listing
July 2021

Mapping groundwater-dependent ecosystems in arid Central Asia: Implications for controlling regional land degradation.

Sci Total Environ 2021 Jul 14;797:149027. Epub 2021 Jul 14.

Eurasia Institute of Earth Sciences, Istanbul Technical University, Maslak, Istanbul 34469, Turkey.

Groundwater-dependent ecosystems (GDEs) exist all over the world, especially in water-limited regions. To achieve better water management, it is necessary to map and identify GDEs. Central Asia (CA) is one of the most arid regions in the mid-latitudes and one of the major regions with shallow groundwater tables. However, the role of groundwater in the impacts of climate change and regional anthropogenic activities on environmental risks, especially regional desertification, is inadequately understood due to the limited available research on GDEs. In the present study, a remote sensing-based method was used for mapping GDEs in regional CA, and three means-overlay analysis, correlation analysis, and the water balance method-were adopted to validate the accuracy of the mapping outcomes. Our results indicated that: 1) GDEs were concentrated around large lakes and in central Kazakhstan (between 46°N and 50°N latitudes), and areas "Very Likely" and "Likely" to be GDEs accounted for 36.89%, and 28.85% of the total natural vegetation areas, respectively; 2) at the watershed scale, the Sarysu Basin had the largest proportion (94.02% of the area) of potential GDEs while the Ysyk-Kol Basin had the lowest proportion (17.84%); 3) all the three validation methods indicated a good performance for our GDE mapping results. We concluded that the remote sensing-based GDE identification method can be considered a potential approach for mapping GDEs regionally. Better recognition of relationships among groundwater availability, ecosystem health and groundwater management policies should be developed by conducting further studies, to protect GDEs and to prevent regional land degradation.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149027DOI Listing
July 2021
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