Publications by authors named "Yang Xu"

3,682 Publications

  • Page 1 of 1

MicroRNA expression profiles in the granulosa cells of infertile patients undergoing progestin primed ovarian stimulation.

Eur J Obstet Gynecol Reprod Biol 2022 Aug 6;276:228-235. Epub 2022 Aug 6.

Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing 100034, China. Electronic address:

Research Questions: Does Progesterone primed ovarian stimulation (PPOS) protocol influence the microRNA expression profiles of granulosa cells of follicular fluid in infertile patients? Will adding progestins in the follicular phase affect follicular microenvironment?

Design: This was a prospective study with granulosa cells collected from patients who received PPOS protocol (n = 15) and who received gonadotropin releasing hormones (GnRH) antagonist protocol (n = 15). MicroRNA sequencing was set to find differently expressed microRNAs. The most significant statistically differential expressed miRNAs are chosen to be validated by quantitative real-time polymerase chain reaction (qRT-PCR) following by target genes prediction and enrichment analysis.

Results: The microRNA sequencing found 24 up-regulated miRNAs and 37 down-regulated miRNA among which 10 microRNAs (miR-4697-5p, miR-6788-5p, miR-6869-5p, miR-4644, miR-199a-5p, miR-6801-3p, miR-146b-5p, miR-4261, miR-199a-3p, miR-150-5p) can be detected in all sequencing samples and rank top enrichment scores in all microRNAs. The PCR validation showed that the down-regulation of miR-4261 and up-regulation of miR-6869-5p were consistent with the sequencing results. Putative genes were identified to be confidently predictive. The enrichment analysis showed that the most enriched gene ontology terms involves cell proliferation and apoptosis and the most enriched pathway included Hippo signaling pathway and Wnt signaling pathway.

Conclusions: PPOS protocol could up-regulate the expression of miR-6869-5p and down-regulate the expression of miR-4261 in granulosa cells of follicular fluid in infertile patients which involves a lot of cellular and molecular mechanisms requiring follow-up studies.
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http://dx.doi.org/10.1016/j.ejogrb.2022.08.001DOI Listing
August 2022

Investigating and modeling the differential DNA methylation for early lung adenocarcinoma diagnosis.

Biomark Med 2022 Aug 11. Epub 2022 Aug 11.

Department of Pathology, State Key Laboratory of Complex Severe and Rare Disease, Molecular Pathology Research Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Aberrant DNA methylations serve as rich sources of diagnostic biomarkers, but a further improvement in their accuracy and clinical utility is warranted. Large panel bisulfite sequencing were performed on paired normal and stage I/IV tumors from 226 lung adenocarcinoma cancer patients to characterize the differentially methylated regions (DMRs). Random forest model achieved high prediction accuracy (sensitivity 96% and specificity 97.56%) to separate normal controls from both early and advanced cancer samples, which is superior to most previous prediction models tested in lung adenocarcinoma. Our results suggest that combining the random forest model with targeted bisulfite sequencing have great clinical potentials to accurately predict and early diagnose lung adenocarcinoma during cancer screening.
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http://dx.doi.org/10.2217/bmm-2022-0240DOI Listing
August 2022

β-Cyclodextrin-Grafted Chitosan Enhances Intestinal Drug Absorption and Its Preliminary Mechanism Exploration.

AAPS PharmSciTech 2022 Aug 10;23(6):221. Epub 2022 Aug 10.

College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China.

β-Cyclodextrin (CD) and chitosan (CS) have attracted great attention due to their unique properties and structures. β-Cyclodextrin-grafted chitosan (CD-CS) has been widely used as a drug carrier to prepare nano-formulations for drug delivery. However, few researches have been conducted to investigate the effect of CD-CS as an excipient on cellular uptake and intestinal absorption. Herein, Caco-2 cells were used to investigate the influence of CD-CS on cellular uptake. The MTT assay showed that CD-CS was non-toxic to Caco-2 cells in concentrations ranging from 15.62 to 125 μg/mL. Confocal laser microscopy and flow cytometry measurements indicated that the uptake ability of Caco-2 cells was significantly enhanced after being treated with CD-CS at a concentration of 31.25 μg/mL or incubation for 0.5 h, and the uptake enhancement gradually increased with increasing CD-CS concentration and incubation time. The Caco-2 monolayer cell model and the everted intestinal sac method were employed to preliminarily explore the mechanism of the improved intestinal absorption. The results demonstrated that CD-CS might open the tight junctions and enhance the clathrin-dependent endocytosis, macro-pinocytosis, and phagocytosis of the intestinal epithelial cells. Such findings can serve as references and inspiration for the design of absorption enhancers.
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http://dx.doi.org/10.1208/s12249-022-02380-zDOI Listing
August 2022

Adverse role of colonial morphology and favorable function of microcystins for Microcystis to compete with Scenedesmus.

Harmful Algae 2022 Aug 30;117:102293. Epub 2022 Jul 30.

Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China. Electronic address:

In eutrophic freshwaters, Microcystis usually becomes dominant in phytoplankton communities due to the synergistic effects of its special eco-physiological traits and environmental factors. Colonial morphology can protect Microcystis from zooplankton grazing, which indirectly favors Microcystis to outcompete other phytoplankton, although the colonial form is not conducive to the absorption of nutrients. Moreover, unicellular Microcystis usually has competitive advantages over other phytoplankton due to its efficient absorption capacity for nutrients and releasing microcystins. However, the consequence of direct competition between toxic colonial Microcystis and green algae without external grazing pressure still remained unknown. In this study, the competition between toxic colonial Microcystis aeruginosa and a common green alga Scenedesmus obliquus was explored. Results showed that: (1) colonial M. aeruginosa had a higher requirement for key macro-nutrient phosphorus than S. obliquus, and thus its population declined and was replaced by S. obliquus eventually; (2) microcystins released by colonial M. aeruginosa inhibited the photosynthetic activity and growth of S. obliquus at early stage of the competition; (3) the photosynthetic potential of colonial M. aeruginosa was stimulated in response to the competitive stress from S. obliquus, although the population of colonial M. aeruginosa declined eventually; (4) microcystin production of colonial M. aeruginosa was enhanced by phosphorus limitation due to S. obliquus competition and was positively related to photosynthetic potential of colonial M. aeruginosa. These results indicated that, in the absence of complex natural environment, colonial Microcystis cannot outcompete Scenedesmus in a pure competition, although microcystins can play a favorable role in the competition, which clarified the opposite role of colonies and microcystins in the competition of colonial Microcystis against other phytoplankton.
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http://dx.doi.org/10.1016/j.hal.2022.102293DOI Listing
August 2022

Efficient Tuning of the Spin-Orbit Torque via the Magnetic Phase Transition of FeRh.

ACS Nano 2022 Aug 9. Epub 2022 Aug 9.

Key Laboratory of Polar Materials and Devices (MOE), School of Physics and Electronic Science, East China Normal University, Shanghai 200241, People's Republic of China.

The understanding and control of the spin-orbit torque (SOT) are central to antiferromagnetic spintronics. Despite the fact that a giant SOT efficiency has been achieved in numerous materials, its efficient tuning in a given material has not been established. Materials with magnetic phase transitions (MPTs) offer a new perspective, as the SOT efficiency may vary significantly for the different magnetic orderings across the transition, and the transition itself can be readily tuned by various control parameters. This work reports that the SOT efficiency of a FeRh-based perpendicular magnetized heterostructure can be significantly tuned by varying the temperature across the MPT. The SOT efficiency exhibits a temperature hysteresis associated with the first-order nature of the MPT, and its value in the ferromagnetic phase is seen to be enhanced by ∼450%, simply by a lowering of temperature to drive FeRh into the antiferromagnetic phase. Furthermore, current-induced magnetization switching can be achieved without an assistant magnetic field for both ferromagnetic and antiferromagnetic FeRh, with a low critical switching current density for the latter. These results not only directly establish FeRh as an efficient spin generator but also present a strategy to dynamically tune SOT via varying the temperature across MPTs.
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http://dx.doi.org/10.1021/acsnano.2c04488DOI Listing
August 2022

Metabolic labeling of the bacterial peptidoglycan by functionalized glucosamine.

iScience 2022 Aug 12;25(8):104753. Epub 2022 Jul 12.

Membrane Biochemistry and Biophysics, Bijvoet Centre for Biomolecular Research, Department of Chemistry, Utrecht University, Padualaan 8, 3584 Utrecht, the Netherlands.

-Acetylglucosamine (GlcNAc) is an essential monosaccharide required in almost all organisms. Fluorescent labeling of the peptidoglycan (PG) on -acetylglucosamine has been poorly explored. Here, we report on the labeling of the PG with a bioorthogonal handle on the GlcNAc. We developed a facile one-step synthesis of uridine diphosphate -azidoacetylglucosamine (UDP-GlcNAz) using the glycosyltransferase OleD, followed by incorporation of GlcNAz into the peptidoglycan precursor Lipid II and fluorescent labeling of the azido group via click chemistry. In a PG synthesis assay, fluorescent GlcNAz-labeled Lipid II was incorporated into peptidoglycan by the DD-transpeptidase activity of bifunctional class A penicillin-binding proteins. We further demonstrate the incorporation of GlcNAz into the PG layer of OleD-expressed bacteria by feeding with 2-chloro-4-nitrophenyl GlcNAz (GlcNAz-CNP). Hence, our labeling method using the heterologous expression of OleD is useful to study PG synthesis and possibly other biological processes involving GlcNAc metabolism .
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http://dx.doi.org/10.1016/j.isci.2022.104753DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356107PMC
August 2022

Triazine-based porous organic polymer as pipette tip solid-phase extraction adsorbent coupled with HPLC for the determination of sulfonamide residues in food samples.

Food Chem 2022 Aug 1;397:133831. Epub 2022 Aug 1.

College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua 321004, China.

In this work, triazine-based porous organic polymer (TAPT-BPDA) synthesized by simple solvothermal method with stable chemical properties was employed as pipette tip solid-phase extraction (PT-SPE) adsorbent for the extraction of sulfonamides for the first time. Under the optimal conditions, good linearities (1-300 μg L, R ≥ 0.9987) and low limits of detection (0.10-0.28 μg L) were obtained. The recoveries of sulfonamides in meat, egg and milk samples were in the range of 76.1-114.0 %. The prepared TAPT-BPDA showed good reusability with the recoveries of sulfonamides remained above 80.0 % after eight recycles. The adsorption mechanism between SAs and adsorbent might be the combined effects of electrostatic interactions, hydrogen bonding and π-π interactions. The results demonstrated potential applications of a TAPT-BPDA-based PT-SPE-HPLC method for the analysis of trace sulfonamide residues in food samples.
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http://dx.doi.org/10.1016/j.foodchem.2022.133831DOI Listing
August 2022

Targeting PERK mediated endoplasmic reticulum stress attenuates neuroinflammation and alleviates lipopolysaccharide-induced depressive-like behavior in male mice.

Int Immunopharmacol 2022 Aug 5;111:109092. Epub 2022 Aug 5.

Clinical Anatomy & Reproductive Medicine Application Institute, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China. Electronic address:

Neuroinflammation plays a key role in the development of depression-like behaviors.Endoplasmic reticulum (ER) stress,defined as accumulation of unfolded proteins in the ER,is suggested tocollaboratewithinflammation process to drive sustained neuroinflammation. Protein kinase R-like endoplasmic reticulum kinase (PERK) is ofparticularly attractive target because it plays key rolein the regulation of ER stress-induced neuroinflammation, however, little isknown whether PERKmediatedER stress is implicated in LPS-induced depression-like behaviors.Thus, we aimed to evaluate the induction of PERK pathwayin mice with depression-like behaviors induced by LPS, as well as the alterations in depression-like behaviorsfollowing the blocking of PERK pathway.We found that LPS challenges resulted in enhanced PERK in the hippocampus, with no alteration in the prefrontal cortex. Importantly, we found that PERKinhibitorISRIB reducedthe proinflammatory responsesof microglia in the context of acute LPS-induced brain inflammation, and subsequent the preserved hippocampal neurogenesis, and improvement in depression-like behavioroutcomes following LPS challenges.It was also worth mentioning thatISRIB treatmentreduced the peripheral pro-inflammatory cytokines includingIL-1β, IL-6 and IL-18. Thus, targetingPERK mediated Endoplasmic reticulum stress may be a promising antidepressant and anti-inflammatory candidate drug for the alleviation of neuroinflammationmediated depression, and PERKinhibitorISRIBmay havebenefits for combating major depressive disorder.
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http://dx.doi.org/10.1016/j.intimp.2022.109092DOI Listing
August 2022

RPP30 is a novel diagnostic and prognostic biomarker for gastric cancer.

Front Genet 2022 19;13:888051. Epub 2022 Jul 19.

Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

This study aimed to identify the hub gene in gastric cancer (GC) tumorigenesis. A biomarker prediction model was constructed and analyzed, and protein expression in histopathological samples was verified in a validation cohort. Differentially expressed genes (DEGs) were identified from GC projects in The Cancer Genome Atlas (TCGA) database. Functional enrichment analysis of DEGs was performed between the high- and low- Ribonuclease P protein subunit p30 (RPP30) expression groups. ROC analysis was performed to assess RPP30 expression to discriminate GC from normal tissues. Functional enrichment pathways and immune infiltration of DEGs were analyzed using GSEA and ssGSEA. Survival analysis and nomogram construction were performed to predict patient survival. Immunohistochemical staining of GC tissues was performed to validate RPP30 expression in GC and paracancerous samples. Gene expression data and clinical information of 380 cases (375 GC samples and 32 para-cancerous tissues) were collected from TCGA database. The AUC for RPP30 expression was found to be 0.785. The G alpha S signaling pathway was the most significantly enriched signaling pathway. Primary therapy outcome ( < 0.001, HR = 0.243, 95% CI = 0.156-0.379), age ( = 0.012, HR = 1.748, 95% CI = 1.133-2.698), and RPP30 expression ( < 0.001, HR = 2.069, 95% CI = 1.346-3.181) were identified as independent prognostic factors. As a quantitative approach, a nomogram constructed based on RPP30 expression, age, and primary therapy outcome performed well in predicting patient survival. Nineteen of the 25 tissue samples from the validation cohort showed positive RPP30 expression in GC tissues, whereas 16 cases showed negative RPP30 staining in normal tissues. The difference between the two was statistically significant. High RPP30 expression was significantly correlated with disease progression and poor survival in GC, promoting tumorigenesis and angiogenesis tRNA dysregulation. This study provides new and promising insights into the molecular pathogenesis of tRNA in GC.
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http://dx.doi.org/10.3389/fgene.2022.888051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343801PMC
July 2022

Potato (Solanum tuberosum L.) non-specific lipid transfer protein StLTP6 promotes viral infection by inhibiting virus-induced RNA silencing.

Planta 2022 Aug 4;256(3):54. Epub 2022 Aug 4.

State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, Shandong, China.

Main Conclusion: For the first time it is reported that members of the nsLTP protein family could promote viral infection by inhibiting virus-induced RNA silencing. Non-specific lipid transfer proteins (nsLTPs) are a class of soluble proteins with low relative molecular weight and widely present in higher plants. The role of nsLTPs in biotic and abiotic stresses has been studied, but no report has shown that nsLTPs play a role in the process of viral infection. We report the function and mechanism of the classical nsLTP protein StLTP6 in viral infection. We found that StLTP6 expression was remarkably upregulated in potato infected with potato virus Y and potato virus S. The infection efficiency and virus content of StLTP6-overexpressed potato and Nicotiana benthamiana were remarkable increased. Further study found that the overexpression of StLTP6 inhibited the expression of multiple genes in the RNA silencing pathway, thereby inhibiting virus-induced RNA silencing. This result indicated that StLTP6 expression was induced during viral infection to inhibit the resistance of virus-induced RNA silencing and promote viral infection. In summary, we reported the role of StLTP6 in viral infection, broadening the biological function range of the nsLTP family and providing valuable information for the study of viral infection mechanism.
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http://dx.doi.org/10.1007/s00425-022-03948-6DOI Listing
August 2022

Corrigendum to "OsIAA20, an Aux/IAA protein, mediates abiotic stress tolerance in rice through an ABA pathway" [Plant Sci. 308 (2021) 110903].

Plant Sci 2022 Aug 2:111403. Epub 2022 Aug 2.

College of Life Science, Hebei Normal University, No.20 Road East. 2nd Ring South, Yuhua District, Shijiazhuang 050024, Hebei, China. Electronic address:

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http://dx.doi.org/10.1016/j.plantsci.2022.111403DOI Listing
August 2022

Immunoinformatic screening of Marburgvirus epitopes and computational investigations of epitope-allele complexes.

Int Immunopharmacol 2022 Aug 1;111:109109. Epub 2022 Aug 1.

Department of Physics, Florida International University, Miami, FL 33199, USA; Biomolecular Sciences Institute, Florida International University, Miami, FL 33199, USA. Electronic address:

Marburgvirus (MARV), a member of the Filovirus family, causes severe hemorrhagic fever in humans. Currently, there are no approved vaccines or post exposure treatment methods available against MARV. With the aim of identifying vaccine candidates against MARV, we employ different sequence-based computational methods to predict the MHC-I and MHC-II T-cell epitopes as well as B-cell epitopes for the complete MARV genome. We analyzed the variations in the predicted epitopes among four MARV variants, the Lake Victoria, Angola, Musoke, and Ravn. We used a consensus approach to identify several epitopes, including novel epitopes, and narrowed down the selection based on different parameters such as antigenicity and IC values. The selected epitopes can be used in various vaccine constructs that give effective antibody responses. The MHC-I epitope-allele complexes for GP and NP with favorably low IC values were investigated using molecular dynamics computations to determine the molecular details of the epitope-allele complexes. This study provides information for further experimental validation of the potential epitopes and the design and development of MARV vaccines.
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http://dx.doi.org/10.1016/j.intimp.2022.109109DOI Listing
August 2022

A Rare Case of Acute Infectious Purpura Fulminans Caused by and Human Herpesvirus Type 5.

J Inflamm Res 2022 26;15:4251-4260. Epub 2022 Jul 26.

Laboratory Bacteria Room, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101, People's Republic of China.

Background: Purpura fulminans (PF), a rare, life-threatening disorder, is a hematological emergency in which there is skin necrosis, disseminated intravascular coagulation (DIC), and protein C deficiency. In PF, the skin necrosis and DIC are secondary to protein C deficiency. This may progress rapidly to multiorgan failure caused by the thrombotic occlusion of small- and medium-sized blood vessels.

Case Report: This article presents the case of a 22-year-old male with fever as well as necrotic and purpuric skin lesions. The ultrasound and computed tomography scans revealed infections in the skin wounds as well as venous microthrombosis and thrombosis in multiple intracranial and pulmonary vessels. The laboratory tests showed signs of sepsis, thrombocytopenia, an abnormal decrease in protein C and antithrombin III, DIC, multiple organ and system failures, gastric varices, and gastrointestinal hemorrhage. The blood, sputum, and secretions under the skin lesions were cultured and were positive for . The results of the high-throughput genetic testing of the pathogenic microorganism DNA were consistent. In addition, human herpesvirus type 5 was detected. The histopathological examination of the skin lesions revealed pathological features consistent with PF. After successful treatment by the departments of Dermatology, Emergency Critical Care Medicine, and the Intensive Care Unit, the patient was discharged after 67 days of hospitalization.

Conclusion: Adults with acquired protein C and/or S deficiency states, including certain bacterial and viral infections, who drink alcohol and take varieties of non-steroidal anti-inflammatory analgesics at the same time, may develop acute infectious PF. Clinicians should be aware of this for early diagnosis and treatment.
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http://dx.doi.org/10.2147/JIR.S369986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340174PMC
July 2022

A guide of column selection for two-dimensional liquid chromatography method development of natural alkaloids.

Talanta 2022 Jul 21;251:123738. Epub 2022 Jul 21.

Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; Jiangxi Provincial Key Laboratory for Pharmacodynamic Material Basis of Traditional Chinese Medicine, Ganjiang Chinese Medicine Innovation Center, Nanchang, 330000, China. Electronic address:

Natural products, especially alkaloids, are one of the most valuable, potential drug leads in drug discovery. As an efficient tool for complex samples, two-dimensional liquid chromatography (2D-LC) has become a powerful means of analysis and separation of natural alkaloids. Method development of 2D-LC is of great importance because it helps to enhance the selectivity, resolution and peak capacity of a separation system. However, due to the diversity of the nature and subclasses of natural alkaloids, peak tailing occurs frequently, making alkaloid separation complicated and time-consuming. To conquer such difficulties, we proposed a guide for column selection and combination in 2D-LC so as to improve the alkaloid separation. Based on a comprehensive evaluation of applicability and orthogonality of several columns, this guide would provide a simple but clear starting point for column selection of 2D-LC method development. The evaluation included seven columns to involve most separation mechanisms reported in alkaloid separation, and 49 natural alkaloid standards of various subclasses and natures. Detailed studies of peak shapes of every column were carried out as well, providing useful references to better understand the peak tailing issues of some analytes on specific columns. Subsequently, a 2D-LC method was developed using our guide to isolate an alkaloid sample from U. rhynchophylla, generating symmetrical peaks and a high orthogonality of 80.3%. Further, this evaluation process would help to have a quick understanding when a new stationary phase is designed.
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http://dx.doi.org/10.1016/j.talanta.2022.123738DOI Listing
July 2022

Air Pollution Health Impact Monitoring and Health Risk Assessment Technology and Its Application - China, 2006-2019.

China CDC Wkly 2022 Jul;4(26):577-581

China CDC Key Laboratory of Environment and Population Health, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing, China.

Air pollution is a significant risk factor contributing to the burden of disease in China. Health risk assessment and management are important to reduce the impact of air pollution on public health. To help formulate standardized health risk assessment techniques, a series of studies were conducted from 2006 to 2019. Through systematic review, study of molecular mechanisms, epidemiological investigation, and health effect monitoring, the overall project established a monitoring and evaluation indicator system, a comprehensive information platform, software for automatic data cleaning, and standardized health risk assessment techniques. Technical specifications have been issued by the National Health Commission for promoting health risk assessments across China. This paper introduces the project, the research approach, its main research accomplishments, innovations, and public health significance, and describes directions for further research.
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http://dx.doi.org/10.46234/ccdcw2022.125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339357PMC
July 2022

Maize-peanut rotational strip intercropping improves peanut growth and soil properties by optimizing microbial community diversity.

PeerJ 2022 28;10:e13777. Epub 2022 Jul 28.

College of Agronomy, Shenyang Agricultural University, Shenyang City, Liaoning Province, China.

Rotational strip intercropping (RSI) of cereals and legumes has been developed and widely carried out to alleviate continuous cropping obstacles, to control erosion and to improve field use efficiency. In this study, a four-year fixed-field experiment was carried out in northeast China with three treatments: continuous cropping of maize, continuous cropping of peanuts and rotational strip intercropping of maize and peanut. The results show that crop rotation improved the main-stem height, branch number, lateral branch length, and yield and quality of peanuts; the yield was the highest in 2018, when it was increased by 39.5%. RSI improved the contents of total N, available N, total P, available P, total K and available K; the content of available N was the highest in 2018, with an increase of 70%. Rhizosphere soil urease and catalase activities were significantly increased and were the highest in 2017, reaching 183.13% and 91.21%, respectively. According to a high-throughput sequencing analysis, the rhizosphere soil bacterial richness and specific OTUs decreased in peanut rhizosphere soil, while the fungal increased. There were differences in the bacterial and fungal community structures; specifically, the abundance of Acidobacteria and Planctomycetes increased among bacteria and the abundance of beneficial microorganisms such as Ascomycota increased among fungi. In conclusion, rotational strip intercropping of maize and peanut increased the yield and quality of peanuts and conducive to alleviating the obstacles facing the continuous cropping of peanuts. Among then, soil physicochemical properties, enzyme activity and microbial diversity were significantly affected the yield of peanut.
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http://dx.doi.org/10.7717/peerj.13777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339216PMC
July 2022

5-Fluorouracil reduces the fibrotic scar via inhibiting matrix metalloproteinase 9 and stabilizing microtubules after spinal cord injury.

CNS Neurosci Ther 2022 Aug 2. Epub 2022 Aug 2.

Institute of Neurological Disease, West China Hospital, Sichuan University & The Research Units of West China, Chinese Academy of Medical Sciences, Chengdu, China.

Aims: Fibrotic scars composed of a dense extracellular matrix are the major obstacles for axonal regeneration. Previous studies have reported that antitumor drugs promote neurofunctional recovery.

Methods: We investigated the effects of 5-fluorouracil (5-FU), a classical antitumor drug with a high therapeutic index, on fibrotic scar formation, axonal regeneration, and functional recovery after spinal cord injury (SCI).

Results: 5-FU administration after hemisection SCI improved hind limb sensorimotor function of the ipsilateral hind paws. 5-FU application also significantly reduced the fibrotic scar formation labeled with aggrecan and fibronectin-positive components, Iba1 /CD11b macrophages/microglia, vimentin, chondroitin sulfate proteoglycan 4 (NG2/CSPG4), and platelet-derived growth factor receptor beta (PDGFRβ) pericytes. Moreover, 5-FU treatment promoted stromal cells apoptosis and inhibited fibroblast proliferation and migration by abrogating the polarity of these cells and reducing matrix metalloproteinase 9 expression and promoted axonal growth of spinal neurons via the neuron-specific protein doublecortin-like kinase 1 (DCLK1). Therefore, 5-FU administration impedes the formation of fibrotic scars and promotes axonal regeneration to further restore sensorimotor function after SCI.
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http://dx.doi.org/10.1111/cns.13930DOI Listing
August 2022

An F-FDG PET/CT radiomics nomogram for differentiation of high-risk and non-high-risk patients of the International Neuroblastoma Risk Group Staging System.

Eur J Radiol 2022 Jul 21;154:110444. Epub 2022 Jul 21.

Department of Nuclear Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China. Electronic address:

Purpose: To develop and validate an F-FDG PET/CT radiomics nomogram for non-invasive differentiation of high-risk and non-high-risk patients of the International Neuroblastoma Risk Group (INRG) Staging System (INRGSS).

Method: One hundred thirty-nine neuroblastoma patients were retrospectively enrolled and classified into a training set (n = 84) and validation set (n = 55). Radiomics features were extracted from F-FDG PET/CT images, a radiomics signature was constructed, and a radiomics score (Rad score) was calculated. Then, univariate and multivariate logistic regression analyses were used to screen out the independent clinical factors and construct the clinical model. A radiomics nomogram was developed based on the Rad score and independent clinical factors. The performance of the clinical model, Rad score, and nomogram was assessed by receiver operating characteristic (ROC) curves, calibration curves, and decision curves analysis (DCA).

Results: Seven radiomics features were selected to build the radiomics signature. The age at diagnosis, the INRG stage, neuron-specific enolase (NSE) and Rad score showed a significant difference between the high-risk and non-high-risk patients. The radiomics nomogram incorporating the Rad score and the above clinical factors demonstrated favorable predictive value for differentiating high-risk from non-high-risk, yielded AUCs of 0.988 and 0.971 in the training and validation sets, respectively. The calibration curves showed that the radiomics nomogram had the goodness of fit, and the DCA demonstrated that the radiomics nomogram was clinically useful.

Conclusions: The radiomics nomogram, which incorporates the Rad score and clinical factors can well predict high-risk and non-high-risk patients of the INRGSS. It may help the disease follow-up and management in clinical practice and assist in personalized and precise treatment of neuroblastoma.
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http://dx.doi.org/10.1016/j.ejrad.2022.110444DOI Listing
July 2022

Uncovering the Heterogeneity and Clinical Relevance of Circulating Tumor-Initiating Cells in Hepatocellular Carcinoma Using an Integrated Immunomagnetic-Microfluidic Platform.

ACS Appl Mater Interfaces 2022 Aug 2. Epub 2022 Aug 2.

Department of Liver Surgery & Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University; Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, 180 Fenglin Road, Shanghai 200032, P. R. China.

Circulating tumor-initiating cells (CTICs) with stem cell-like properties play pivotal roles in tumor metastasis and recurrence. However, little is known about the biology and clinical relevance of CTICs in hepatocellular carcinoma (HCC). Here, we investigated the molecular heterogeneity and clinical relevance of CTICs in HCC using a novel integrated immunomagnetic-microfluidic platform (iMAC). We constructed the iMAC and evaluated its ability to detect CTICs using a series of spiked cell experiments. A four-channel microfluidic chip was applied to investigate the composition of CTICs in patients with primary and recurrent HCC utilizing microbeads labeled with one of four stem-related markers: epithelial cell adhesion molecule (EpCAM), CD133, CD90, and CD24. The dynamic changes of these four CTIC subsets were serially monitored during treatment courses. Finally, single-cell RNA profiling was used to reveal the molecular characteristics of the four CTIC subsets. The iMAC platform detected significantly more EpCAM CTICs in the blood samples from 33 HCC patients than the FDA-approved CellSearch system (0.92 ± 0.94 0.23 ± 0.36, < 0.001). The number of EpCAM CTICs (≥0.75/mL) detected by iMAC was a predictor of early recurrence ( = 0.007). The distinct stem-related markers' expression of CTICs could distinguish primary HCC, recurrent HCC, and TACE-resistant HCC. Single-cell transcriptional profiling proved the heterogeneity among individual CTICs and separated the four CTIC subsets into distinct phenotypes. Dissecting the heterogeneity of CTICs using the iMAC represents a novel and informative method for accurate CTIC detection and characterization. This innovative technology will enable more indepth cancer biology research and clinical cancer management than is currently available.
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http://dx.doi.org/10.1021/acsami.2c09085DOI Listing
August 2022

A tunable bilayer Hubbard model in twisted WSe.

Nat Nanotechnol 2022 Aug 1. Epub 2022 Aug 1.

School of Applied and Engineering Physics, Cornell University, Ithaca, NY, USA.

Moiré materials with flat electronic bands provide a highly controllable quantum system for studies of strong-correlation physics and topology. In particular, angle-aligned heterobilayers of semiconducting transition metal dichalcogenides with large band offset realize the single-band Hubbard model. Introduction of a new layer degree of freedom is expected to foster richer interactions, enabling Hund's physics, interlayer exciton condensation and new superconducting pairing mechanisms to name a few. Here we report competing electronic states in twisted AB-homobilayer WSe, which realizes a bilayer Hubbard model in the weak interlayer hopping limit for holes. By layer-polarizing holes via a perpendicular electric field, we observe a crossover from an excitonic insulator to a charge-transfer insulator at a hole density of ν = 1 (in units of moiré density), a transition from a paramagnetic to an antiferromagnetic charge-transfer insulator at ν = 2 and evidence for a layer-selective Mott insulator at 1 < ν < 2. The unique coupling of charge and spin to external electric and magnetic fields also manifests a giant magnetoelectric response. Our results establish a new solid-state simulator for the bilayer Hubbard model Hamiltonian.
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http://dx.doi.org/10.1038/s41565-022-01180-7DOI Listing
August 2022

Recurrent Novel Translocations in B-Lymphoblastic Leukemia/Lymphoma.

Front Oncol 2022 14;12:896858. Epub 2022 Jul 14.

National Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Translocations involving the immunoglobulin heavy chain () locus are common abnormalities in B-lymphoblastic leukemia/lymphoma (B-ALL) and multiple myeloma. These rearrangements result in a juxtaposition of enhancers to the vicinity of oncogenes, such as and , leading to the upregulation of oncogenes. Here, we identified recurrent novel translocations in three B-ALL patients by transcriptome sequencing. Noncoding exon 1 of was translocated to different sites of the gene, resulting in transcripts of , , and . However, a high expression level of truncated was observed in the patients compared with healthy donors, which might be related to the aggressive clinical course and inferior outcome. In summary, we described recurrent novel translocations with high expression levels of , which may contribute to the guidelines for clinical diagnosis and treatment.
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http://dx.doi.org/10.3389/fonc.2022.896858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330356PMC
July 2022

Identification of an m6A-Related Long Noncoding RNA Risk Model for Predicting Prognosis and Directing Treatments in Patients With Colon Adenocarcinoma.

Front Cell Dev Biol 2022 13;10:910749. Epub 2022 Jul 13.

Department of Liver Surgery, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

N6-methyladenosine (m6A) and lncRNAs have been implicated in the development of colon cancer, including tumorigenesis, migration, and invasion. However, the specific effect of m6A regulators on lncRNAs is not clear, and m6A-related lncRNAs may be new prognostic biomarkers and may help direct treatment and medication. We identified 29 prognostic m6A-related lncRNAs and constructed a risk model using 12 lncRNAs. The model was an independent prognostic factor and could accurately predict the prognosis. A stable and robust nomogram that combined the model and pathologic stage was constructed. A total of 2,424 differentially expressed genes (DEGs) were identified based on the model. Functional analysis of the DEGs showed that they were associated with tumor progression, helping investigate the underlying biological functions and signaling pathways of the risk model. In addition, the low-risk group based on the risk model had more sensitivity to afatinib, metformin, and GW.441756, and patients with low risk would more likely respond to immunotherapy. Moreover, patients with higher risk were more sensitive to olaparib, bexarotene, and doxorubicin.
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http://dx.doi.org/10.3389/fcell.2022.910749DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326028PMC
July 2022

Neural-Network-Based Model-Free Calibration Method for Stereo Fisheye Camera.

Front Bioeng Biotechnol 2022 14;10:955233. Epub 2022 Jul 14.

School of Automation, Wuhan University of Technology, Wuhan, China.

The fisheye camera has a field of view (FOV) of over 180°, which has advantages in the fields of medicine and precision measurement. Ordinary pinhole models have difficulty in fitting the severe barrel distortion of the fisheye camera. Therefore, it is necessary to apply a nonlinear geometric model to model this distortion in measurement applications, while the process is computationally complex. To solve the problem, this paper proposes a model-free stereo calibration method for binocular fisheye camera based on neural-network. The neural-network can implicitly describe the nonlinear mapping relationship between image and spatial coordinates in the scene. We use a feature extraction method based on three-step phase-shift method. Compared with the conventional stereo calibration of fisheye cameras, our method does not require image correction and matching. The spatial coordinates of the points in the common field of view of binocular fisheye camera can all be calculated by the generalized fitting capability of the neural-network. Our method preserves the advantage of the broad field of view of the fisheye camera. The experimental results show that our method is more suitable for fisheye cameras with significant distortion.
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http://dx.doi.org/10.3389/fbioe.2022.955233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334662PMC
July 2022

Morphological design of occlusal wear facets for the mandibular first molar crown using different bite registration methods.

J Prosthodont 2022 Jul 30. Epub 2022 Jul 30.

Center of Digital Dentistry, Faculty of Prosthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health, PR China.

Purpose: To compare the accuracy of occlusal wear facet morphology designed using different bite registration methods for mandibular first molar crowns.

Materials And Methods: The posterior teeth and intraoral intercuspal occlusions of 12 participants were scanned. The abutment shape of the mandibular first molars for complete crowns was virtually designed, and the anatomic coping design method was used to design crowns. In the anatomic coping design, digital wax patterns were constructed by elevating the wear facets on the original surface of the first molars and then adjusting the facets with 2 types of virtual occlusions determined by buccal bite registration (BBR) or segmented tooth registration (STR) methods, where the displacement of teeth under bite force was considered (STR) or not (BBR). The occlusal distance between the original wear facets and the antagonists as well as 3D deviations between the facets on the designed crowns and on the surfaces of the original teeth were measured. Paired-samples t-test was used to analyze the results (α = 0.05).

Results: Regarding occlusal distance, the mean 3D deviation and the root mean square (RMS) values of BBR were greater than those of the STR groups (p < 0.001 and p = 0.008). The mean 3D deviations of the crowns of the BBR and STR groups were 0.19 ±0.04 mm and 0.14 ±0.06 mm, respectively and the RMS values were 0.22 ±0.03 mm and 0.18 ±0.04 mm (p < 0.001), respectively.

Conclusions: The morphology of occlusal wear facets of mandibular first molar crowns designed with the occlusion constructed using the segmented tooth registration method are more coincident with the original morphology.
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http://dx.doi.org/10.1111/jopr.13574DOI Listing
July 2022

Lake reclamation alters molecular-level characteristics of lacustrine dissolved organic matter - A study of nine lakes in the Yangtze Plain, China.

Water Res 2022 Jul 19;222:118884. Epub 2022 Jul 19.

Guangdong Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, 1088 Xueyuan Blvd, Xili, Nanshan, Shenzhen, Guangdong 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China. Electronic address:

In recent decades, the reclamation of lakes has captured 42% of the total lake area of the Yangtze Plain in China and introduced additional pressure on lacustrine water quality. While lacustrine dissolved organic matter (DOM) is critical in regulating biogeochemical processing and aquatic biodiversity, the impact of reclamation on the molecular-level characteristics of lacustrine DOM remains unexplored. Here, the DOM characteristics altered by reclamation practices in the Yangtze Plain lakes were investigated using fluorescence spectroscopy, nuclear magnetic resonance spectroscopy, and Fourier transform ion cyclotron resonance mass spectrometry. Results demonstrated that reclamation not only elevated the quantity (on average +32%) but also altered the characteristics and composition of lacustrine DOM. Compared to the natural water sites close by, reclamation sites did not significantly alter the DOM aromaticity but significantly lowered the average molecular weight and increased the biolability of DOM. The chromophoric DOM and humic-like fluorescent components were remarkably elevated, but not the protein-like fluorescent components. More lipid-like and condensed aromatic-like components were detected in the lacustrine DOM as compared to the lignin-like, carbohydrate-like, and protein-like components, which may be driven by the increased microbial processing. Overall, the significant alteration in characteristics and composition of lacustrine DOM highlights the potential impact of reclamation on the DOM biogeochemical cycle and the environmental quality in aquatic ecosystems.
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http://dx.doi.org/10.1016/j.watres.2022.118884DOI Listing
July 2022

Novel LncRNA ZFHX4-AS1 as a Potential Prognostic Biomarker That Affects the Immune Microenvironment in Ovarian Cancer.

Front Oncol 2022 12;12:945518. Epub 2022 Jul 12.

Department of Gynecology, Harbin Medical University Cancer Hospital, Harbin, China.

Background: Ovarian cancer (OvCa) is a malignant disease of the female reproductive system with a high mortality rate. LncRNA has been confirmed to play a crucial role in the development and progression of various cancer types. Novel lncRNA ZFHX4-AS1 has been reported in several cancers, albeit its functional mechanisms in OvCa remain unclear.

Methods: With reference to the public databases and based on integrating bioinformatics analyses, we explored the expression of ZFHX4-AS1 and its roles in the prognosis of OvCa. We employed the Kaplan-Meier curves to investigate the outcome of patients with different ZFHX4-AS1 expressions. Furthermore, its biological function and the related hallmark pathways were assessed through Gene Ontology (GO) annotation, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, and Gene-set enrichment analysis (GSEA). We explored the correlation between lncRNA ZFHX4-AS1 and tumor-infiltrating immune cells through CIBERSORT. The immune checkpoints associated with lncRNA ZFHX4-AS1 and its related genes were investigated. The effect of lncRNA ZFHX4-AS1 on proliferation, invasion and migration of OvCa cells was verified through Cell Counting Kit (CCK)-8, colony formation, wound healing and transwell assays.

Results: The expression of lncRNA ZFHX4-AS1 was upregulated in OvCa relative to that in normal tissues. Increased lncRNA ZFHX4-AS1 expression was associated with poor overall survival and progression-free survival in OvCa. The GO and KEGG pathway analyses revealed the role of lncRNA ZFHX4-AS1 in cell metabolism, protein synthesis, cell proliferation, and cell cycle. GSEA indicated the hallmark gene sets that were significantly enriched in the high and low expression groups. The CIBERSORT database revealed M2 macrophages, memory B-cells, naïve B cells, and activated NK cells were affected by lncRNA ZFHX4-AS1 expression (all < 0.05). The expression of lncRNA ZFHX4-AS1 and its related differential genes , , and were significantly correlated with the immune checkpoints. Knockdown of lncRNA ZFHX4-AS1 could inhibit the proliferation, invasion and migration of OvCa cells.

Conclusions: The results suggested that lncRNA ZFHX4-AS1 is a novel prognostic biomarker associated with cell proliferation, metabolism, infiltration, and distribution of tumor-infiltrating immune cells in OvCa, indicating that lncRNA ZFHX4-AS1 can be used as a potential therapeutic target for OvCa in the future.
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http://dx.doi.org/10.3389/fonc.2022.945518DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315108PMC
July 2022

Clinical Application of Different Vertical Position Tests for Posterior Canal-Benign Paroxysmal Positional Vertigo-Cupulolithiasis.

Front Neurol 2022 12;13:930542. Epub 2022 Jul 12.

Department of Neurology, The First Hospital of Hebei Medical University, Shijiazhuang, China.

Background: Posterior canal-benign paroxysmal positional vertigo-cupulolithiasis (PC-BPPV-cu) is a new and controversial type of benign paroxysmal positional vertigo (BPPV). At present, there are few relevant clinical studies as to whether the Half Dix-Hallpike test (Half D-HT) induces more obvious nystagmus than the Dix Hallpike test (D-HT) and straight head hanging test (SHH) in patients with PC-BPPV-cu.

Objectives: To investigate the clinical characteristics of PC-BPPV-cu, and analyze the diagnostic significance of the Dix-Hallpike test (D-HT), Half D-HT, and straight head hanging (SHH) test in these patients.

Methods: A total of 46 patients with PC-BPPV-cu were enrolled, and divided into two groups ( = 23): a group A (induction order: D-HT, Half D-HT, SHH) and a group B (induction order: Half D-HT, D-HT, SHH).

Results: Among 46 patients with PC-BPPV-cu, the bilateral and unilateral abnormality rates of the disease side were 5 cases and 41 cases, respectively. There were significant differences in the proportion of torsional-upbeating nystagmus and upbeating nystagmus among the three headhanging positions in 46 patients with PC-BPPV-cu ( < 0.001). The slow phase velocity (SPV) of induced nystagmus at half D-HT supine position was slower than D-HT supine position ( < 0.05) and SHH supine position ( < 0.05). The nystagmus latency of D-HT supine position was significantly shorter than half D-HT ( < 0.05) and SHH ( < 0.05). PC-BPPV-cu patients were accompanied by 53.5% semicircular canal paresis, 69.6% audiological abnormalities, 76% cervical vestibular evoked myogenic potential (cVEMP), and 75% video head impulse test (vHIT) abnormalities, the concordance rates of the four detection methods were similar (χ = 0.243, = 0.970).

Conclusions: The Half D-HT is simple and feasible, but might have a risk of false-negative diagnoses of the torsional-upbeating nystagmus and upbeating nystagmus. The D-HT is still a classic induction method for PC-BPPV-cu. The two complement each other and may aid in the diagnosis of PC-BPPV-cu patients. Future clinical applications of Half D-HT require extensive research to determine its diagnostic efficacy.
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http://dx.doi.org/10.3389/fneur.2022.930542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315446PMC
July 2022

Undercarboxylated Osteocalcin and Its Associations With Bone Mineral Density, Bone Turnover Markers, and Prevalence of Osteopenia and Osteoporosis in Chinese Population: A Cross-Sectional Study.

Front Endocrinol (Lausanne) 2022 8;13:843912. Epub 2022 Jul 8.

Shanghai Clinical Research Center of Bone Disease, Department of Osteoporosis and Bone Diseases, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Objective: Undercarboxylated osteocalcin (ucOC) is one form of osteocalcin lacking full carboxylation, which plays an important role in bone homeostasis, glucose homeostasis, and energy metabolism. Our aim is to obtain the profile of serum ucOC level according to gender and age and explore its associations with bone mineral density (BMD), bone turnover markers (BTMs), and prevalence of osteopenia and osteoporosis in the Chinese population.

Methods: This is a cross-sectional study with 900 subjects, composed of 431 men and 469 women. Clinical information was collected, and BMD values of the lumbar spine (L1-4), left femoral neck, and total hip were scanned. Biochemical markers including hepatic and renal function, serum calcium, serum phosphorus, procollagen type 1 N-propeptide (P1NP) β-CrossLaps of type I collagen-containing cross-linked C-telopeptide (β-CTX) intact parathyroid hormone (PTH), 25-hydroxyvitamin D (25OHD), and ucOC were measured.

Results: We found that the median ucOC level was higher in men than women [men, 2.6 ng/ml; women, 1.6 ng/ml; < 0.001]. The profile according to age showed that ucOC levels were the lowest at the age of 40-49 years in both men [2.55 ng/ml (95% CI = 1.96-3.13 ng/ml)] and women [1.57 ng/ml (95% CI = 1.12-2.03 ng/ml)]; in patients younger than 49 years, they decreased with age; then over 50 years, they quickly increased. Furthermore, we found that a higher ucOC level was correlated with lower BMD values at the lumbar spine (men, r = -0.128, = 0.013; women, r = -0.321, < 0.001), femoral neck (men, r = -0.095, = 0.062; women, r = -0.260, < 0.001), and total hip (men, r = -0.123, = 0.015; women, r = -0.209, < 0.001) and higher P1NP (men, r = 0.307, < 0.001; women, r = 0.239, < 0.001) and β-CTX (men, r = 0.169, = 0.001; women, r = 0.354, < 0.001) levels in both men and women. Furthermore, we also showed that a 1 - SD increase in ucOC was associated with an odds ratio (OR) of 1.63 and 1.70 for having osteopenia or osteoporosis in men and women, respectively (men, 95% CI = 1.25-2.13, = 0.004; women, 95% CI = 1.19-2.42, = 0.004).

Conclusions: We first revealed the profile of serum ucOC levels according to gender and age in the Chinese population and demonstrated the associations of ucOC with BMD and BTMs and the risk of prevalent osteopenia or osteoporosis. Our findings provide a clue to elucidate the function of ucOC in bone metabolism.
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http://dx.doi.org/10.3389/fendo.2022.843912DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309304PMC
July 2022

Bio-Inspired Hierarchical Micro-/Nanostructures for Anti-Icing Solely Fabricated by Metal-Assisted Chemical Etching.

Micromachines (Basel) 2022 Jul 7;13(7). Epub 2022 Jul 7.

ZJU-UIUC Institute, International Campus, Zhejiang University, Haining 314400, China.

We report a cost-effective and scalable methodology for producing a hierarchical micro-/nanostructured silicon surface solely by metal-assisted chemical etching. It involves two major processing steps of fabricating micropillars and nanowires separately. The process of producing micro-scale structures by masked metal-assisted chemical etching was optimized. Silicon nanowires were created on the micropillar's surface via maskless metal-assisted chemical etching. The hierarchical micro-/nanostructured surface exhibits superhydrophobic properties with a high contact angle of ~156° and a low sliding angle of <2.5° for deionized water. Furthermore, due to the existence of microscale and nanoscale air trapped at the liquid/solid interface, it exhibits a long ice delay time of 2876 s at -5 °C, more than 5 times longer than that of smooth surfaces. Compared to conventional dry etching methods, the metal-assisted chemical etching approach excludes vacuum environments and high-temperature processes and can be applied for applications requiring hierarchical micro-/nanostructured surfaces or structures.
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http://dx.doi.org/10.3390/mi13071077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317431PMC
July 2022

Altered Regional Activity and Network Homogeneity within the Fronto-Limbic Network at Rest in Medicine-Free Obsessive-Compulsive Disorder.

Brain Sci 2022 Jun 29;12(7). Epub 2022 Jun 29.

Department of Psychiatry, Qiqihar Medical University, Qiqihar 161006, China.

Functional abnormalities in brain areas within the fronto-limbic network have been widely reported in obsessive-compulsive disorder (OCD). However, region- and network-level brain activities of the fronto-limbic network at rest have not been simultaneously investigated in OCD. In this study, 40 medicine-free and non-comorbidity patients with OCD and 38 age-, education-, and gender-matched healthy controls (HCs) underwent a resting-state functional magnetic-resonance-imaging scan. Fractional amplitude of low-frequency fluctuations (fALFF), network homogeneity (NH), and support vector machine were used to analyze the data. Patients with OCD showed increased fALFF in the right orbital frontal cortex (OFC), increased NH in the left OFC, and decreased NH in the right putamen. Decreased NH of the right putamen was negatively correlated with the Y-BOCS total and compulsive behavior scores. Furthermore, a combination of NH in the left OFC and right putamen could be applied to differentiate OCD from HCs with optimum specificity and sensitivity. The current findings emphasize the crucial role of the fronto-limbic network in the etiology of OCD.
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http://dx.doi.org/10.3390/brainsci12070857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312952PMC
June 2022
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