Publications by authors named "Yang Wang"

6,480 Publications

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Mobile tigecycline resistance gene tet(X4) persists with different animal manure composting treatments and fertilizer receiving soils.

Chemosphere 2022 Aug 8:135866. Epub 2022 Aug 8.

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

The emergence of plasmid-mediated tigecycline-resistant genes [tet (×3) to tet (×6)] in animals and humans has raised serious concerns over their possible cross-environmental dissemination. However, behavior of these emerging mobile tet(X)-variant genes in manure treatment processes, particularly for different composting treatments, has not yet been studied. Here, we explored the environmental behavior of mobile tet(X)-variant genes in two typical manure composting treatments and amended soils based on a large-scale molecular investigation across eight provinces in China. Results showed that tet (×4) was the predominant mobile tet(X)-variant gene in fresh manure, natural and thermophilic composting products with both the highest detection frequency (82.5% ± 14.7%), and absolute abundance of tet (×4) [4.26 ± 0.09) × 10] copies/g dry weight, followed by tet (×3), tet (×6), and tet (×5). The occurrence of all mobile tet(X)-variant genes, particularly tet (×4), in receiving soil following composting fertilizer application indicated their transmission from manure to soil. Paired-sampling strategy revealed no significant reduction in mobile tet(X)-variant genes by natural composting, while thermophilic composting exhibited clear efficacy in removing tet(X)-variant genes. After thermophilic composting, tet (×4) exhibited the lowest reduction (94.1%) compared with other mobile tet(X)-variant genes (96.9%-99.9%), which may be attributable to its significant correlation with ISCR2 (P < 0.05) facilitating its transfer to various hosts including persisted thermotolerant bacteria. Thus, tet (×4) showed better persistence in livestock-related environments. Collectively, this study highlights the importance of controlling the environmental dissemination of clinically relevant mobile tet(X)-variant genes by establishing a sound process and operational strategy.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135866DOI Listing
August 2022

Visit-to-visit glycated hemoglobin A1c variability in adults with type 2 diabetes: a systematic review and meta-analysis.

Chin Med J (Engl) 2022 Mar 3. Epub 2022 Mar 3.

Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.

Background: Current practice uses the latest measure of glycated hemoglobin (HbA1c) to facilitate clinical decision-making. Studies have demonstrated that HbA1c variability links the risk of death and complications of diabetes. However, the role of HbA1c variability is unclear in clinical practice. This systematic review summarized the evidence of visit-to-visit HbA1c variability regarding different metrics in micro- and macro-vascular complications and death in people with type 2 diabetes.

Methods: We searched PubMed, EMBASE (via OVID), and Cochrane Central Register (CENTRAL, via OVID) for studies investigating the association between HbA1c variability and adverse outcomes in patients with type 2 diabetes and performed random-effects meta-analysis stratified by HbA1c variability metrics in terms of standard deviation (SD), coefficient of variation (CV), and HbA1c variability score (HVS).

Results: In people with type 2 diabetes, the highest quantile of all three HbA1c variability metrics (HbA1c-standard deviation [HbA1c-SD], HbA1c-coefficient of variance [HbA1c-CV], and HVS) is associated with increased risks of all-cause mortality, cardiovascular events, progression to chronic kidney disease, amputation, and peripheral neuropathy. For example, the hazard ratio of HbA1c-SD on all-cause mortality was 1.89 with 95% confidence interval (95% CI) 1.46-2.45 (HbA1c-CV 1.47, 95% CI 1.26-1.72; HVS 1.67, 95% CI 1.34-2.09).

Conclusions: High HbA1c variability leads to micro- and macro-vascular complications of type 2 diabetes and related death. People with type 2 diabetes and high HbA1c variability need additional attention and care for the potential adverse outcomes.
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http://dx.doi.org/10.1097/CM9.0000000000002073DOI Listing
March 2022

Enhanced effect of combining bone marrow mesenchymal stem cells (BMMSCs) and pulsed electromagnetic fields (PEMF) to promote recovery after spinal cord injury in mice.

MedComm (2020) 2022 Sep 3;3(3):e160. Epub 2022 Aug 3.

Rehabilitation Medicine Center and Institute of Rehabilitation Medicine, West China Hospital Sichuan University Chengdu PR China.

Spinal cord injury (SCI) is a destructive traumatic disease of the central nervous system without satisfying therapy efficiency. Bone marrow mesenchymal stem cells (BMMSCs) therapy promotes the neurotrophic factors' secretion and axonal regeneration, thereby promoting recovery of SCI. Pulsed electromagnetic fields (PEMF) therapy has been proven to promote neural growth and regeneration. Both BMMSCs and PEMF have shown curative effects for SCI; PEMF can further promote stem cell differentiation. Thus, we explored the combined effects of BMMSCs and PEMF and the potential interaction between these two therapies in SCI. Compared with the SCI control, BMMSCs, and PEMF groups, the combinational therapy displayed the best therapeutic effect. Combinational therapy increased the expression levels of nutritional factors including brain-derived neurotrophic factor (BDNF), nerve growth factors (NGF) and vascular endothelial growth factor (VEGF), enhanced neuron preservation (NeuN and NF-200), and increased axonal growth (MBP and myelin sheath). Additionally, PEMF promoted the expression levels of BDNF and VEGF in BMMSCs via Wnt/β-catenin signaling pathway. In summary, the combined therapy of BMMSCs and PEMF displayed a more satisfactory effect than BMMSCs and PEMF therapy alone, indicating a promising application of combined therapy for the therapy of SCI.
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http://dx.doi.org/10.1002/mco2.160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350428PMC
September 2022

Associations between isoflavone exposure and reproductive damage in adult males: evidence from human and model system studies.

Biol Reprod 2022 Aug 10. Epub 2022 Aug 10.

Key Lab of Medical Protection for Electromagnetic Radiation, Ministry of Education of China, Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Gaotanyan St 30, Chongqing 400038, China.

It's controversial whether exposure to isoflavones, constituents of certain plants such as soy bean, exerts male reproductive toxicity. This study was designed to investigate whether isoflavone exposure during adulthood could have deleterious impacts on male reproductive health by the cross-sectional study, animal experiments, and in vitro tests. In the cross-sectional study, we observed that urinary isoflavones were not significantly associated with semen quality including sperm concentrations, sperm count, progressive motility, and total motility, respectively (All P-value for trend>0.05). However, negative associations were found between plasma testosterone and urinary Σisoflavones, genistein, glycitein, and dihydrodaidzein (all P-value for trend <0.05). In the animal experiments, serum and intratesticular testosterone levels were decreased in mice exposed to several dosages of genistein. Genistein administration caused up-regulation of estrogen receptor alpha (ERα) and down-regulation of cytochrome P45017A1 (CYP17A1) protein levels in testes of mice. However, genistein treatment during adulthood did not induce appreciable structural damages of reproductive system in mice. In vitro tests, we observed that genistein of different dosages (0.01, 2.5, 10 μM) caused a concentration dependent inhibition of testosterone production by TM3 Leydig cells (half-maximal inhibitory concentration = 3.796 nM, P < 0.05). Elevated protein expression of ERα and decreased mRNA/protein level of CYP17A1 were also observed in genistein-treated cells. Protein level of CYP17A1 and testosterone concentration were significantly restored in the ERα siRNA-transfected cells, compared to cells that treated with genistein alone (P < 0.05). The results demonstrate that exposure to isoflavones during adulthood may be associated with alterations of reproductive hormones. Particularly for genistein, which inhibits testosterone biosynthesis through up-regulation of ERα in Leydig cells of mice, might induce the disruption of testosterone production in human. The present study provides novel perspective into potential targets for male reproductive compromise induced by isoflavone exposure.
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http://dx.doi.org/10.1093/biolre/ioac157DOI Listing
August 2022

Temperate and tropical lizards are vulnerable to climate warming due to increased water loss and heat stress.

Proc Biol Sci 2022 Aug 10;289(1980):20221074. Epub 2022 Aug 10.

Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.

Climate warming has imposed profound impacts on species globally. Understanding the vulnerabilities of species from different latitudinal regions to warming climates is critical for biological conservation. Using five species of lizards as a study system, we quantified physiological and life-history responses and geography range change across latitudes under climate warming. Using integrated biophysical models and hybrid species distribution models, we found: (i) thermal safety margin is larger at high latitudes and is predicted to decrease under climate warming for lizards at all latitudes; (ii) climate warming will speed up embryonic development and increase annual activity time of adult lizards, but will exacerbate water loss of adults across all latitudes; and (iii) species across latitudes are predicted to experience habitat contraction under climate warming due to different limitations-tropical and subtropical species are vulnerable due to increased extremely high temperatures, whereas temperate species are vulnerable due to both extremely high temperatures and increased water loss. This study provides a comprehensive understanding of the vulnerability of species from different latitudinal regions to climate warming in ectotherms, and also highlights the importance of integrating environmental factors, behaviour, physiology and life-history responses in predicting the risk of species to climate warming.
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http://dx.doi.org/10.1098/rspb.2022.1074DOI Listing
August 2022

Posterior temporary fixation of C1-C2 screw-rod system for unstable C1 burst fracture.

Medicine (Baltimore) 2022 Aug;101(31):e29385

Department of Spine Surgery, Ningbo No. 6 Hospital, Ningbo, China.

Whether an unstable C1 burst fracture should be treated surgically or conservatively is controversial. The purpose of this study is to evaluate the effectiveness and motion-preserving function of temporary fixation of C1-C2 screw-rod system for the reduction and fixation of unstable C1 burst fracture. We retrospectively reviewed 10 patients who were treated with posterior C1-C2 temporary fixation without fusion. We assessed age at surgery, gender, pre- and postoperative visual analog scale (VAS), Neck Disability Index (NDI), atlanto-dens interval (ADI), lateral mass distance (LMD), and rotation function of C1-C2 complex. Six males and 4 females were included in our study. The average follow-up duration was 14.1 ± 1.37 months. The left-to-right ROMs of C1-C2 rotation was 9.6° ± 1.42°. The preoperative cervical VAS was 8.30 ± 0.48; the postoperative cervical VAS of C1-C2 fusion was 2.90 ± 0.57. The preoperative VAS for removal was 2.0 ± 0.00, and the postoperative VAS for removal was 2.3 ± 0.48. The preoperative cervical NDI was 81.40% ± 2.07%, the postoperative cervical NDI of C1-C2 fusion was 18.10% ± 1.52%. The preoperative NDI for removal was 15.9% ± 1.20%. The postoperative NDI for removal was 14.5% ± 1.08%. The preoperative ADI was 4.43 ± 0.34 mm, and postoperative ADI was 1.94 ± 0.72 mm. The preoperative LMD was 6.36 ± 0.58 mm, and postoperative LMD was 1.64 ± 0.31 mm. Posterior temporary C1-C2 fixation can achieve a good fusion and satisfied reduction of C1 fracture, relieve the pain, improve the cervical function outcome, but may reduce the rotational range of motion of C1-C2. Posterior C1-C2 temporary fixation without fusion was not suitable for C1 burst fracture. We recommend permanent C1-C2 fixation and fusion for C1 burst fracture if surgery is necessary.
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http://dx.doi.org/10.1097/MD.0000000000029385DOI Listing
August 2022

Visible-light-driven photocatalytic degradation of ofloxacin by BiOBr nanocomposite modified with oxygen vacancies and N-doped CQDs: Enhanced photodegradation performance and mechanism.

Chemosphere 2022 Aug 6:135976. Epub 2022 Aug 6.

Beijing Key Laboratory for Source Control Technology of Water Pollution, Engineering Research Center for Water Pollution Source Control and Eco-remediation, Beijing Forestry University, Beijing, 100083, China.

The rapid recombination of photogenerated carriers and weak light absorption capacity are two major challenges for bismuth-based photocatalysts. Here, N-CQDs/BiOBr micro-flower photocatalysts with the visible-light activity were fabricated through the ethylene glycol solvothermal method for the first time, and oxygen vacancies (OVs) and N-doped carbon quantum dots (N-CQDs) were simultaneously introduced on the surface of BiOBr. OVs were introduced to form defective BiOBr (BiOBr). N-CQDs and BiOBr formed a strong binding effect. Then, the composition, morphology, crystal structure and photoelectric property of photocatalysts were studied, and the mechanism and pathway of ofloxacin (OFL) photodegradation were studied. N-CQDs/BiOBr-4 was a micro-flower composed of nanosheets with a thickness of about 60 nm, this structure produced multiple light reflections. Photoelectrochemical analysis confirmed that the synergistic effect of OVs and N-CQDs significantly promoted the electron-hole separation (3 times vs BiOBr) and enhanced the light absorption range (Eg = 2.96 eV vs 3.24 eV). Meanwhile, the removal rate of OFL by N-CQDs/BiOBr-4 was 6 times higher than that by BiOBr (K of N-CQDs/BiOBr-4 was 32 times higher than that of BiOBr). Electron spin resonances analysis and radical quenching experiments showed that ·O and h played dominant roles in the OFL photodegradation system, and their contribution rates were 89.84% and 70.31%, respectively. There were main degradation pathways for OFL, including oxidation, dealkylation, hydroxylation and decarboxylation. This study explored the synergistic and complementary effects between OVs and N-CQDs, and provided a promising strategy for the photodegradation of toxic antibiotics by visible-light-driven photocatalysts.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135976DOI Listing
August 2022

Safety and Efficacy of Single Condylar Knee Prosthesis When Treating Knee Single Compartment Osteoarthritis: A Prospective, Case-Randomized Controlled Study.

Comput Intell Neurosci 2022 30;2022:3722619. Epub 2022 Jul 30.

Department of Orthopedics, Caidian District People's Hospital, Wuhan 430100, Hubei Province, China.

Objective: The aim of this study is to explore the safety and efficacy of single condylar knee prosthesis when treating knee single compartment osteoarthritis by measuring the decrease of hemoglobin, total postoperative blood loss, maximum reduction of HCT, and knee joint activity.

Methods: A total of 80 patients with knee joint single compartment osteoarthritis treated in our hospital from January 2020 to December 2021 were studied. They were randomly assigned to a study group ( = 40) and a control group ( = 40). The study group was treated with total knee prosthesis, while the control group was treated with simple knee prosthesis. The decrease rate of hemoglobin, the amount of bleeding, and the maximum decrease of hematocrit were compared after treatment. The range of motion of knee joint was evaluated by the Fugl-Meyer motor function scale (FM-B) and Berg balance scale (BBS).

Results: The decrease of hemoglobin in the study group at 24 hours, 36 hours, and 48 hours after treatment was remarkably lower ( < 0.05). The total blood loss and the maximum reduction of hematocrit(HCT) in the study group were lower ( < 0.05). The range of motion (ROM) of the knee joint in the study group at 6 and 12 months after treatment was remarkably higher than that before treatment and remarkably higher compared to the control group ( < 0.05). The FM-B scale and BBS scale of the studied cohort at 6 and 12 months after treatment were remarkably higher than those before treatment and were remarkably higher compared to the control's ( < 0.05).

Conclusion: The unicondylar knee prosthesis is safer and more effective in the treatment of noncompartmental osteoarthritis of the knee, facilitating less trauma and perioperative blood loss and enhancing the patient's motion and balance.
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http://dx.doi.org/10.1155/2022/3722619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356790PMC
August 2022

A 33-residue peptide tag increases solubility and stability of Escherichia coli produced single-chain antibody fragments.

Nat Commun 2022 Aug 8;13(1):4614. Epub 2022 Aug 8.

Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.

Single-chain variable fragments (scFvs), composed of variable domains of heavy and light chains of an antibody joined by a linker, share antigen binding capacity with their parental antibody. Due to intrinsically low solubility and stability, only two Escherichia coli-produced scFvs have been approved for therapy. Here we report that a 33-residue peptide, termed P17 tag, increases the solubility of multiple scFvs produced in Escherichia coli SHuffle strain by up to 11.6 fold. Hydrophilic sequence, especially charged residues, but not the predicted α-helical secondary structure of P17 tag, contribute to the solubility enhancement. Notably, the P17 tag elevates the thermostability of scFv as efficiently as intra-domain disulfide bonds. Moreover, a P17-tagged scFv targeting hepatitis B virus surface proteins shows over two-fold higher antigen-binding affinity and virus-neutralizing activity than the untagged version. These data strongly suggest a type I intramolecular chaperone-like activity of the P17 tag. Hence, the P17 tag could benefit the research, production, and application of scFv.
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http://dx.doi.org/10.1038/s41467-022-32423-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359998PMC
August 2022

Dual-Level Training of Gaussian Processes with Physically Inspired Priors for Geometry Optimizations.

J Chem Theory Comput 2022 Aug 8. Epub 2022 Aug 8.

Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, United States.

Gaussian process (GP) regression has been recently developed as an effective method in molecular geometry optimization. The prior mean function is one of the crucial parts of the GP. We design and validate two types of physically inspired prior mean functions: force-field-based priors and posterior-type priors. In this work, we implement a dual-level training (DLT) optimizer for the posterior-type priors. The DLT optimizers can be considered as a class of optimization algorithms that belong to the delta-machine learning paradigm but with several major differences compared to the previously proposed algorithms in the same paradigm. In the first level of the DLT, we incorporate the classical mechanical descriptions of the equilibrium geometries into the prior function, which enhances the performance of the GP optimizer as compared to the one using a constant (or zero) prior. In the second level, we utilize the surrogate potential energy surfaces (PESs), which incorporate the physics learned in the first-level training, as the prior function to refine the model performance further. We find that the force-field-based priors and posterior-type priors reduce the overall optimization steps by a factor of 2-3 when compared to the limited-memory Broyden-Fletcher-Goldfarb-Shanno (L-BFGS) optimizer as well as the constant-prior GP optimizer proposed in previous works. We also demonstrate the potential of recovering the real PESs with GP with a force-field prior. This work shows the importance of including domain knowledge as an ingredient in the GP, which offers a potentially robust learning model for molecular geometry optimization and for exploring molecular PESs.
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http://dx.doi.org/10.1021/acs.jctc.2c00546DOI Listing
August 2022

Transcatheter arterial chemoembolization is safe and effective for patients with late-stage or recurrent oral carcinoma.

Front Oncol 2022 22;12:831583. Epub 2022 Jul 22.

Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Objective: We reported the long-term outcomes of transcatheter chemoembolization (TACE) for patients with late-stage or recurrent oral carcinoma.

Methods: This retrospective study enrolled 18 patients with late-stage or recurrent oral carcinoma between December 2015 and April 2021. The tumor-feeding artery was catheterized, and cisplatin/oxaliplatin and 5-FU/raltitrexed were infused with embolization using polyvinyl alcohol or gelatin sponge. Computed tomography was performed at about 1, 3, and 6 months after the procedure, and every 6 months after that. During the procedure and follow-up, procedure outcomes, complications, treatment efficacy, and overall survival were analyzed.

Results: A total of 31 sessions of TACE were performed, with a technical success rate of 100%. Of 12 patients combined with oral hemorrhage, two patients showed rebleeding 35 and 37 days later, with a clinical efficiency of hemostasis of 88.9%. Mild complications were observed in 11 patients (61.1%). Severe complications or procedure-related deaths were not observed during or after the procedure. The objective response rate and disease control rate were 20.0% and 86.7%, 38.5% and 61.5%, and 25.0% and 50.0% at 1, 3, and 6 months later, respectively. Seventeen patients (94.4%) were followed up, with a median duration of 37.8 months (IQR 22.3-56.8). Nine patients died of tumor progression, one died of massive rebleeding, and one died of severe lung infection. The median overall survival was 23.8 months.

Conclusion: TACE is a safe and effective procedure with minimal invasiveness for treating late-stage or recurrent oral carcinoma. TACE can be recommended as a palliative treatment, particularly for patients with oral hemorrhage.
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http://dx.doi.org/10.3389/fonc.2022.831583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353522PMC
July 2022

Homogenization of an acoustic coating with a steel backing subject to an obliquely incident sound.

J Acoust Soc Am 2022 Jul;152(1):624

Laboratory of Science and Technology on Integrated Logistics Support, College of Intelligence Science and Technology, National University of Defense Technology, Changsha 410073, China.

An effective homogenization model for the acoustic coating of underwater structures is important for reducing the complexity of acoustic scattering computation, which arises from the huge difference in scale between the integral structure and the inhomogeneous microstructure of the coating. The main difficulty of this homogenization arises from the oblique-incidence effect of external sound waves and the interface effect between the coating and backing. In this work, a hybrid method, combining the Bloch wave analysis and retrieval technique, is proposed to characterize the acoustic behavior of the voided coating backed with a steel plate under the action of external sound waves with an arbitrary incident angle. The effectiveness of this method is validated by numerical simulations and comparison with the Bloch wave method and the traditional retrieval method. The influence of the shear-wave effect under obliquely incident sound waves and the coupling effect between the coating and the backing on the homogenization model is investigated in detail, providing a comprehensive understanding of the effective acoustic behavior of the coating.
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http://dx.doi.org/10.1121/10.0012189DOI Listing
July 2022

Directional Manipulation of Electron Transfer by Energy Level Engineering for Efficient Cathodic Oxygen Reduction.

Nano Lett 2022 Aug 5. Epub 2022 Aug 5.

School of Chemistry and Materials Science, Collaborative Innovation Center of Chemistry for Energy Materials, University of Science and Technology of China, Hefei, Anhui 230026, P.R. China.

Electron transfer plays an important role in determining the energy conversion efficiency of energy devices. Nitrogen-coordinated single metal sites (M-N) materials as electrocatalysts have exhibited great potential in devices. However, there are still great difficulties in how to directionally manipulate electron transfer in M-N catalysts for higher efficiency. Herein, we demonstrated the mechanism of electron transfer being affected by energy level structure based on classical iron phthalocyanine (FePc) molecule/carbon models and proposed an energy level engineering strategy to manipulate electron transfer, preparing high-performance ORR catalysts. Engineering molecular energy level via modulating FePc molecular structure with nitro induces a strong interfacial electronic coupling and efficient charge transfer from carbon to FePc-β-NO molecule. Consequently, the assembled zinc-air battery exhibits ultrahigh performance which is superior to most of M-N catalysts. Energy level engineering provides a universal approach for directionally manipulating electron transfer, bringing a new concept to design efficient and stable M-N electrocatalyst.
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http://dx.doi.org/10.1021/acs.nanolett.2c01933DOI Listing
August 2022

EGF promotes PKM2 O-GlcNAcylation by stimulating O-GlcNAc transferase phosphorylation at Y976 and their subsequent association.

J Biol Chem 2022 Aug 2:102340. Epub 2022 Aug 2.

Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, 130024, China. Electronic address:

Epidermal growth factor (EGF) is one of the most well-characterized growth factors and plays a crucial role in cell proliferation and differentiation. Its receptor EGFR has been extensively explored as a therapeutic target against multiple types of cancers, such as lung cancer and glioblastoma. Recent studies have established a connection between deregulated EGF signaling and metabolic reprogramming, especially rewiring in aerobic glycolysis, which is also known as the Warburg effect and recognized as a hallmark in cancer. Pyruvate kinase M2 (PKM2) is a rate-limiting enzyme controlling the final step of glycolysis and serves as a major regulator of the Warburg effect. We previously showed that PKM2 T405/S406 O-GlcNAcylation, a critical mark important for PKM2 de-tetramerization and activity, was markedly upregulated by EGF. However, the mechanism by which EGF regulates PKM2 O-GlcNAcylation still remains uncharacterized. Here, we demonstrated that EGF promoted O-GlcNAc transferase (OGT) binding to PKM2 by stimulating OGT Y976 phosphorylation. As a consequence, we found PKM2 O-GlcNAcylation and de-tetramerization were upregulated, leading to a significant decrease in PKM2 activity. Moreover, distinct from PKM2, we observed that the association of additional phosphotyrosine binding proteins with OGT was also enhanced when Y976 was phosphorylated. These proteins included STAT1, STAT3, STAT5, PKCδ, and p85, which are reported to be O-GlcNAcylated. Together, we show EGF-dependent Y976 phosphorylation is critical for OGT-PKM2 interaction and propose that this post-translational modification might be important for substrate selection by OGT.
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http://dx.doi.org/10.1016/j.jbc.2022.102340DOI Listing
August 2022

Stereo- and Regioselectivity of Hydrogenation of a Recently Synthesized Carboncone and Its Predictive Models.

J Org Chem 2022 Aug 5. Epub 2022 Aug 5.

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, China.

Since its atomically precise synthesis in recent experiments, the carboncone molecule presents a novel example of discrete nanocarbons with promising applications, but little is known yet about its chemical properties. In this work, we present a comprehensive computational study on the hydrogenation of carboncone with a varying number of added H atoms (from 1 to 12). Unlike planar benzenoid hydrocarbons, carboncone prefers that all H atoms be added to its external, convex surface. The previous topology-based model for hydrogenated fullerenes and benzenoid hydrocarbons is shown to be no longer valid for carboncone. We here propose an extended model capable of predicting the hydrogenation regioselectivity for carboncone, which is largely governed by π delocalization. Yet the H···H repulsion at rim sites also plays an important role in adduct stability. Interestingly, some preferred addition patterns can be understood by counting the size of intact π rings upon H addition. These findings may provide insightful guidance to the functionalization of carboncones and related nanocarbons.
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http://dx.doi.org/10.1021/acs.joc.2c00970DOI Listing
August 2022

Second-line treatment options in advanced thymic carcinoma after failure of platinum-based chemotherapy: A multicenter retrospective study.

Cancer Med 2022 Aug 4. Epub 2022 Aug 4.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Thoracic Oncology, Peking University Cancer Hospital & Institute, Beijing, China.

Background: Currently there is no standard therapy recommended for second-line treatment for thymic carcinoma. Our study compared multidrug chemotherapy, single-agent chemotherapy, and PD-1 inhibitors in patients diagnosed with advanced thymic carcinoma who had previous platinum-based chemotherapy at the clinic.

Methods: The study included patients with thymic carcinoma who failed first-line platinum-based chemotherapy. Kaplan-Meier methods were applied in the study for estimating the progression-free survival (PFS) and overall survival (OS) curves. Pearson chi-square or Fisher's exact chi-square test was adopted to make comparisons of the objective response rate (ORR) between treatment groups. Cox regression was used for the multivariate analyses in PFS and OS.

Results: Among the 92 patients enrolled, multidrug chemotherapy was used in 51 (55.4%) patients for second-line therapy. Thirty-six patients (35.9%) received single-agent chemotherapy, and eight patients (8.7%) underwent PD-1 inhibitors. The multidrug chemotherapy group showed better efficacy than the other two groups, with an ORR of 35.3% (p = 0.006). The median PFS of multidrug chemotherapy, single-agent chemotherapy and PD-1 inhibitors were 5.0 months, 3.0 months, and 4.0 months, respectively (p = 0.008). Patients in the multidrug chemotherapy group also showed an advantage in OS in comparison with the other two treatment groups (p = 0.045), with a median OS of 30.4 months. Multivariate analysis showed that second-line treatment was independent factor for both PFS (p = 0.035) and OS (p = 0.037). Grade 3-4 AEs were mostly detected in patients receiving multidrug chemotherapy and were primarily hematologic. Treatment-related mortality was not found in any of the groups.

Conclusions: Multidrug chemotherapy had a trend toward a more positive response rate and outcomes in longer survival time than single-agent chemotherapy and PD-1 inhibitors. Multidrug chemotherapy is a choice worth considering for second-line therapy in patients with thymic carcinoma if tolerable.
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http://dx.doi.org/10.1002/cam4.5053DOI Listing
August 2022

Investigation on the evolution of hydrothermal biochar.

Chemosphere 2022 Jul 31;307(Pt 2):135774. Epub 2022 Jul 31.

Shenzhen Automotive Research Institute, Beijing Institute of Technology, Shenzhen, 518118, Guangdong, China.

The objective of this study was to visualize trends and current research status of hydrothermal biochar research through a bibliometric analysis by using CiteSpace software. The original article data were collected from the Web of Science core database published between 2009 and 2020. A visual analysis network of national co-authored, institutional co-authored and author co-authored articles was created, countries, institutions and authors were classified accordingly. By visualizing the cited literature and journal co-citation networks, the main subject distribution and core journals were identified respectively. By visualizing journal co-citations, the main research content was identified. Further the cluster analysis revealed the key research directions of knowledge structure. Keyword co-occurrence analysis and key occurrence analysis demonstrate current research hotspots and new research frontiers. Through the above analysis, the cooperation and contributions of hydrothermal biochar research at different levels, from researchers to institutions to countries to macro levels, were explored, the disciplinary areas of knowledge and major knowledge sources of hydrothermal biochar were discovered, and the development lineage, current status, hotspots and trends of hydrothermal biochar were clarified. The results obtained from the study can provide a reference for scholars to gain a deeper understanding of hydrothermal biochar.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135774DOI Listing
July 2022

Crosstalk between arterial components and bioresorbable, 3-D printed poly-L-lactic acid scaffolds.

Biomater Sci 2022 Aug 3. Epub 2022 Aug 3.

Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, 174 Shazheng Street, Chongqing 400030, People's Republic of China.

Bioresorbable scaffolds (BRSs) are designed to provide a temporary support that subsequently leaves behind native vessels after its complete degradation. The accumulation of mechanical changes influences the vascular histological characteristics and , leading to crosstalk and various behaviors in BRSs in different arterial components, which is different from that observed in traditional metal stents. Hence, we analyzed typical elastic and muscular arteries, the abdominal aorta of Sprague-Dawley rats and carotid arteries of New Zealand rabbits, after both received 3-D printed poly-L-lactic acid BRSs. We observed a lower level of scaffold degradation and severe intimal hyperplasia in the carotid arteries of rabbits because of the synthetic phenotypic transformation of vascular smooth muscle cells (SMCs) and endothelial-to-mesenchymal transition of endothelial cells (ECs). Extracellular matrix remodeling and endothelial repair occurred in a less rapid manner in the abdominal aorta of rats. These results suggest that muscular arterial components such as SMCs and ECs are more sensitive to BRS degradation-induced mechanical changes compared to those of elastic arteries. Therefore, the rat abdominal aorta might be more suitable for assessing BRS degradation and safety, while the carotid artery of rabbits could be used to evaluate drug coatings on BRSs, as it closely reflects the recovery of ECs and proliferation of SMCs. Our study also confirmed that the histological characteristics of vasculature should be considered while choosing an animal model for BRS evaluation.
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http://dx.doi.org/10.1039/d2bm00732kDOI Listing
August 2022

Efficacy and safety of a treatment in patients with locoregionally advanced nasopharyngeal carcinoma (LANC) involving carotid artery invasion.

Eur Arch Otorhinolaryngol 2022 Aug 2. Epub 2022 Aug 2.

College of Otolaryngology Head and Neck Surgery, National Clinical Research Center for Otolaryngologic Diseases, Key Lab of Hearing Science, Ministry of Education, Beijing Key Lab of Hearing Impairment for Prevention and Treatment, Medical School of Chinese PLA, 28 Fuxing Road, Beijing, 100853, People's Republic of China.

Purpose: Locally advanced nasopharyngeal carcinoma (LANC) often invades the parapharyngeal space and internal carotid artery. Are patients with LANC invading carotid artery are at risk of massive neck hemorrhage after radiotherapy?

Methods: This retrospective study included 130 LANC patients with carotid artery invasion admitted to our hospital between January 2012 and September 2019. All patients were treated with induction chemotherapy followed by concurrent chemoradiotherapy (CCRT) ± epidermal growth factor receptor (EGFR) inhibitor. Univariate and multivariate analysis of risk factors were conducted for the prognosis and the occurrence of massive neck hemorrhage of LANC patients with carotid artery invasion.

Outcomes: The 5-year progression-free survival (PFS), distant metastasis-free survival (DMFS), local nodal recurrence-free survival (LNRFS), local recurrence-free survival (LRFS), nodal recurrence-free survival (NRFS) and overall survival (OS) of the 130 patients were 75.2%, 76.8%, 90.0%, 93.9%, 95.8% and 87.2%, respectively. The incidence of fatal bleeding after radiotherapy was 2.3% (3/130). The primary site of the three cases were all the pharyngeal recess, with more than 270° carotid artery invasion, suffering nasopharyngeal necrosis after radiotherapy (2 of which were diabetics and 1 received re-radiation after recurrence). Univariate analysis showed that clinical stage was negatively correlated with DMFS and PFS (P < 0.05). The induction chemotherapy TP regimen, platinum-based concurrent chemotherapy and EGFR inhibitors (Nituzumab/Cetuximab) significantly improved PFS and DMFS (P < 0.05). Patients with hemoglobin levels > 110 g/L had significantly inferior PFS, DMFS and OS than those with hemoglobin levels ≤ 110 g/L (P < 0.05). Multivariate analysis showed that the EGFR inhibitor was an independent risk factor for PFS and DMFS, while the lowest hemoglobin level was an independent risk factor for OS.

Conclusions: In LANC patients whose carotid artery invasion was < 270°, induction chemotherapy (IC) followed by helical tomotherapy (HT) and concurrent chemoradiotherapy (CCRT) with EGFR (epidermal growth factor receptor) inhibitor had mild and tolerable side effects, better PFS and DMFS, with no massive hemorrhage. In patients whose primary tumor was pharyngeal recess with carotid artery invasion ≥ 270°, poorly controlled diabetes or re-radiotherapy led to a higher risk of massive hemorrhage after radiotherapy.
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http://dx.doi.org/10.1007/s00405-022-07446-zDOI Listing
August 2022

1D and 3D co-simulation and self-adaptive position control of electrostatic levitation in China's Space Station.

NPJ Microgravity 2022 Aug 2;8(1):29. Epub 2022 Aug 2.

State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, China Academy of Sciences, Shanghai, China.

The greatest challenge of electrostatic levitation for containerless material processing is the stable control of charged material during heating. Recently, high-precision self-adaptive control of electrostatic levitation has been achieved in China's Space Station. Based on the 1D and 3D co-simulation analysis, an optimal scheduling of control strategies of sample release and retrieval in space is developed. Both simulation results and on-orbit experiments demonstrated that the inversion of surface charge is responsible for the heating induced material instability. On-orbit experiments indicated that under laser illuminations, the net surface charge of metal Zr changed from positive to negative at 900 K and from negative to positive at 1300 K. The possible physical mechanism of the charge inversion of heated material is discussed.
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http://dx.doi.org/10.1038/s41526-022-00215-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345915PMC
August 2022

Preclinical and Clinical Amelioration of Bone Fractures with Mesenchymal Stromal Cells: a Systematic Review and Meta-Analysis.

Cell Transplant 2022 Jan-Dec;31:9636897211051743

Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.

Even though reunion of bone fracture confronts clinicians, mesenchymal stromal cells (MSCs) are investigated to be curative in bone fracture. This study aimed to explore the application potential of MSCs for healing bone fractures. By inputting search terms and retrieving studies published up to March 2021, multiple databases, including PubMed, EMBASE, Web of Science, and Cochrane Library, were searched to identify eligible studies. The mean difference (MD) and 95% confidence interval (95% CI) were calculated to analyze the main results in the meta-analysis. Data analysis was performed using Engauge Digitizer 10.8 and R Software. Of the 31 articles, 26 were preclinical studies ( = 913), and 5 were clinical trials ( = 335). Preclinically, MSCs therapy significantly augmented the progress of bone regeneration [(bone volume over tissue volume (MD7.35, 0.01)], despite some non-significant effects (on the callus index, bone strength, work to failure, and stiffness). Clinically, the MSC group had a significantly reduced incidence of poor recovery (odds ratio (OR) 0.30, 0.01); however, a significant decrease in healing time was not observed in the MSC group (MD 2.47, = 0.26). In summary, our data suggest that patients with bone fractures benefited from MSC administration and that MSCs are a potentially useful agent for bone regeneration. Despite these satisfactory outcomes, larger randomised clinical trials (RCTs) are necessary to confirm these findings.
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http://dx.doi.org/10.1177/09636897211051743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350497PMC
August 2022

P38α MAPK is a gatekeeper of uterine progesterone responsiveness at peri-implantation via Ube3c-mediated PGR degradation.

Proc Natl Acad Sci U S A 2022 Aug 1;119(32):e2206000119. Epub 2022 Aug 1.

Fujian Provincial Key Laboratory of Reproductive Health Research, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, Fujian, 361102, China.

Estrogen and progesterone specify the establishment of uterine receptivity mainly through their respective nuclear receptors, ER and PR. PR is transcriptionally induced by estrogen-ER signaling in the endometrium, but how the protein homeostasis of PR in the endometrium is regulated remains elusive. Here, we demonstrated that the uterine-selective depletion of P38α derails normal uterine receptivity ascribed to the dramatic down-regulation of PR protein and disordered progesterone responsiveness in the uterine stromal compartment, leading to defective implantation and female infertility. Specifically, Ube3c, an HECT family E3 ubiquitin ligase, targets PR for polyubiquitination and thus proteasome degradation in the absence of P38α. Moreover, we discovered that P38α restrains the polyubiquitination activity of Ube3c toward PR by phosphorylating the Ube3c at serine741 . In summary, we provided genetic evidence for the regulation of PR protein stability in the endometrium by P38α and identified Ube3c, whose activity was modulated by P38α-mediated phosphorylation, as an E3 ubiquitin ligase for PR in the uterus.
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http://dx.doi.org/10.1073/pnas.2206000119DOI Listing
August 2022

Performance enhancement of nitrogen-polar GaN-based light-emitting diodes prepared by metalorganic chemical vapor deposition.

Opt Lett 2022 Aug;47(15):3628-3631

Nitrogen-polar (N-polar) III-nitride materials have great potential for application in long-wavelength light-emitting diodes (LEDs). However, the poor quality of N-polar nitride materials hinders the development of N-polar devices. In this work, we report the enhanced performance of N-polar GaN-based LEDs with an optimized InGaN/GaN double quantum well (DQW) structure grown by metalorganic chemical vapor deposition. We improved the quality of the N-polar InGaN/GaN DQWs by elevating the growth temperature and introducing hydrogen as the carrier gas during the growth of the quantum barrier layers. N-polar LEDs prepared based on the optimized InGaN/GaN DQWs show significantly enhanced (by over 90%) external quantum efficiency and a weakened droop effect compared with a reference LED. More importantly, the optimized N-polar DQWs show a significantly longer emission wavelength than Ga-polar DQWs grown at the same QW growth temperature. This work provides a feasible approach to improving the quality of the N-polar InGaN/GaN QW structure, and it will promote the development of N-polar GaN-based long-wavelength light-emitting devices for micro-LED displays.
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http://dx.doi.org/10.1364/OL.463618DOI Listing
August 2022

Enzymatic Production of Chondroitin Oligosaccharides and Its Sulfate Derivatives.

Front Bioeng Biotechnol 2022 13;10:951740. Epub 2022 Jul 13.

The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.

Chondroitin sulfate (CS) has a wide range of physiological functions and clinical applications. However, the biosynthesis of chondroitin oligosaccharides (o-CHs) and sulfate derivatives with specific length is always challenging. Herein, we report enzymatic strategies for producing homogeneous o-CHs and its sulfate derivatives from microbial sourced chondroitin. Chondroitin disaccharides, tetrasaccharides, hexasaccharides, octasaccharides, and decasaccharides with defined structure were produced by controllably depolymerizing microbial sourced chondroitin with an engineered chondroitinase ABC I. The highest conversion rates of the above corresponding o-CHs were 65.5%, 32.1%, 12.7%, 7.2%, and 16.3%, respectively. A new efficient enzymatic sulfation system that directly initiates from adenosine 5'-triphosphate (ATP) and sulfate was developed and improved the sulfation of chondroitin from 8.3% to 85.8% by optimizing the temperature, sulfate and ATP concentration. o-CHs decasaccharide, octasaccharide, hexasaccharide, tetrasaccharide and disaccharide were modified and the corresponding sulfate derivatives with one sulfate group were prepared. The enzymatic approaches constructed here for preparing o-CHs and its sulfate derivatives pave the way for the study of structure-activity relationship and applications.
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http://dx.doi.org/10.3389/fbioe.2022.951740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326237PMC
July 2022

NAFLD aggravates acute pancreatitis through bacterial translocation and cholesterol metabolic dysregulation in the liver and pancreas in mice.

Hepatobiliary Pancreat Dis Int 2022 Jul 20. Epub 2022 Jul 20.

Department of Gastroenterology, Peking University People's Hospital, Beijing 100044, China; Clinical Center of Immune-Mediated Digestive Diseases, Peking University People's Hospital, Beijing 100044, China. Electronic address:

Background: Nonalcoholic fatty liver disease (NAFLD) is an independent risk factor for severe acute pancreatitis (AP). The underlying mechanism remains unclear. We sought to determine how bacterial translocation and cholesterol metabolism in the liver and pancreas affect the severity of AP in NAFLD mice.

Methods: C57BL/6N mice were fed on a high-fat diet (HFD) to generate the NAFLD model, and mice in the control group were provided with a normal diet (ND). After being anesthetized with ketamine/xylazine, mice got a retrograde infusion of taurocholic acid sodium into the pancreatic duct to induce AP, and sham operation (SO) was used as control. Serum amylase and Schmidt's pathological score system were used to evaluate AP severity. Bacterial loads, total cholesterol level, and cholesterol metabolic-associated molecules [low-density lipoprotein receptor (LDLR) and ATP-binding cassette transporter A1 (ABCA1)] were analyzed in the liver and pancreas.

Results: Compared with the ND-AP group, mice in the HFD-AP group had severer pancreatitis, manifested with higher serum amylase levels and higher AP pathologic scores, especially the inflammation and hemorrhage scores. Compared with the HFD-SO group and ND-AP group, bacterial loads in the liver and pancreas were significantly higher in the HFD-AP group. Mice in the HFD-AP group showed a decreased LDLR expression and an increased ABCA1 expression in the pancreas, although there was no significant difference in pancreas total cholesterol between the HFD-AP group and the ND-AP group.

Conclusions: NAFLD aggravates AP via increasing bacterial translocation in the liver and pancreas and affecting pancreas cholesterol metabolism in mice.
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http://dx.doi.org/10.1016/j.hbpd.2022.07.004DOI Listing
July 2022

A phase II open label, single arm study of hypofractionated stereotactic radiotherapy with chemoradiotherapy using intensity-modulated radiotherapy for newly diagnosed glioblastoma after surgery: the HSCK-010 trial protocol.

BMC Cancer 2022 Jul 29;22(1):827. Epub 2022 Jul 29.

CyberKnife Center, Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, 12 Wulumuqi Road (M), Shanghai, 200040, China.

Background: The most frequently diagnosed primary brain tumor is glioblastoma (GBM). Nearly all patients experience tumor recurrence and up to 90% of which is local recurrence. Thus, increasing the therapeutic ratio of radiotherapy using hypofractionated stereotactic radiotherapy (HSRT) can reduce treatment time and may increase tumor control and improve survival. To evaluate the efficacy and toxicity of the combination of HSRT and intensity-modulated radiotherapy (IMRT) with temozolomide after surgery in GBM patients and provide evidence for further randomized controlled trials.

Methods/design: HSCK-010 is an open-label, single-arm phase II trial (NCT04547621) which includes newly diagnosed GBM patients who underwent gross total resection. Patients will receive the combination of 30 Gy/5fx HSRT, and 20 Gy/10fx IMRT adjuvant therapy with concurrent temozolomide and adjuvant chemotherapy. The primary endpoint is overall survival (OS). Secondary outcomes include progression-free survival (PFS) rate, objective-response rate (ORR), quality of life (Qol) before and after the treatment, cognitive function before and after the treatment, and rate of treatment-related adverse events (AE). The combination of HSRT and IMRT with temozolomide can benefit the patients after surgery with good survival, acceptable toxicity, and reduced treatment time.

Trial Registration: NCT04547621 . Registered on 14 September 2020.
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http://dx.doi.org/10.1186/s12885-022-09914-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335974PMC
July 2022

Pyro-catalysis for tooth whitening via oral temperature fluctuation.

Nat Commun 2022 Jul 29;13(1):4419. Epub 2022 Jul 29.

Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, 100081, China.

Tooth whitening has recently become one of the most popular aesthetic dentistry procedures. Beyond classic hydrogen peroxide-based whitening agents, photo-catalysts and piezo-catalysts have been demonstrated for non-destructive on-demand tooth whitening. However, their usage has been challenged due to the relatively limited physical stimuli of light irradiation and ultrasonic mechanical vibration. To address this challenge, we report here a non-destructive and convenient tooth whitening strategy based on the pyro-catalysis effect, realized via ubiquitous oral motion-induced temperature fluctuations. Degradation of organic dyes via pyro-catalysis is performed under cooling/heating cycling to simulate natural temperature fluctuations associated with intake and speech. Teeth stained by habitual beverages and flavorings can be whitened by the pyroelectric particles-embedded hydrogel under a small surrounding temperature fluctuation. Furthermore, the pyro-catalysis-based tooth whitening procedure exhibits a therapeutic biosafety and sustainability. In view of the exemplary demonstration, the most prevalent oral temperature fluctuation will enable the pyro-catalysis-based tooth whitening strategy to have tremendous potential for practical applications.
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http://dx.doi.org/10.1038/s41467-022-32132-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338087PMC
July 2022

Biomechanical analysis of atlantoaxial intraarticular fusion cages with posterior pedicle screws fixation using finite element method.

Clin Biomech (Bristol, Avon) 2022 Jul 21;98:105735. Epub 2022 Jul 21.

Ningbo Sixth Hospital, 1059#, Zhongshan Dong Road, Ningbo City, Zhejiang Province, PR China.

Background: Cadaveric biomechanical studies indicated that atlantoaxial intraarticular fusion cages with posterior pedicle screws fixation could increase the multi-axial rigidity. However, the stress distribution of the fixation construct is still unclear.

Methods: From computed tomography images, a nonlinear intact three-dimensional C0-2 finite element model was developed and validated. Four finite element models were reconstructed: intact model, unstable model, bilateral atlantoaxial pedicle screws combined bilateral cages model, bilateral atlantoaxial pedicle screws model. The range of motion and maximum von Mises stresses were compared under flexion, extension, lateral bending, and axial rotation.

Findings: Compared with unstable model, both bilateral atlantoaxial pedicle screws combined bilateral cages model and bilateral atlantoaxial pedicle screws model fixation techniques reduced range of motion by >99% in extension, flexion, lateral bending and axial rotation. For bilateral atlantoaxial pedicle screws combined bilateral cages model, the maximum von Mises stress was in the base of the C2 screw head site. In the bilateral atlantoaxial pedicle screws model was stressed at the rod linked C1 and C2 screws. Compared with bilateral atlantoaxial pedicle screws model, bilateral atlantoaxial pedicle screws combined bilateral cages model reduced the maximum von Mises stress on the implants by >90% in extension, flexion, lateral bending and axial rotation.

Interpretation: The finite element model study indicated that, compared with posterior C1-C2 pedicle screws fixation, atlantoaxial intraarticular fusion cages with posterior pedicle screws fixation could not only significantly restore stability to the atlantoaxial junction, but also dramatically reduce the maximum von Mises stress in the C1-C2 pedicle screws.
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http://dx.doi.org/10.1016/j.clinbiomech.2022.105735DOI Listing
July 2022

Momentum-matching and band-alignment van der Waals heterostructures for high-efficiency infrared photodetection.

Sci Adv 2022 Jul 29;8(30):eabq1781. Epub 2022 Jul 29.

State Key Laboratory of Infrared Physics, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China.

Two-dimensional (2D) infrared photodetectors always suffer from low quantum efficiency (QE) because of the limited atomically thin absorption. Here, we reported 2D black phosphorus (BP)/BiOSe van der Waals (vdW) photodetectors with momentum-matching and band-alignment heterostructures to achieve high QE. The QE was largely improved by optimizing the generation, suppressing the recombination, and improving the collection of photocarriers. Note that momentum-matching BP/BiOSe heterostructures in -space lead to the highly efficient generation and transition of photocarriers. The recombination process can be largely suppressed by lattice mismatching-immune vdW interfaces. Furthermore, type II BP/BiOSe vdW heterostructures could also assist fast transport and collection of photocarriers. By constructing momentum-matching and band-alignment heterostructures, a record-high QE of 84% at 1.3 micrometers and 76.5% at 2 micrometers have been achieved in BP/BiOSe vdW photodetectors.
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http://dx.doi.org/10.1126/sciadv.abq1781DOI Listing
July 2022

Plasma Dermatology: Skin Therapy Using Cold Atmospheric Plasma.

Front Oncol 2022 12;12:918484. Epub 2022 Jul 12.

Department of Surgery, Department of Dermatology, Shanghai Fourth People's Hospital, and School of Medicine, Tongji University, Shanghai, China.

Cold atmospheric plasma-based plasma medicine has been expanding the diversity of its specialties. As an emerging branch, plasma dermatology takes advantage of the beneficial complexity of plasma constituents (e.g., reactive oxygen and nitrogen species, UV photons, and electromagnetic emission), technical versatility (e.g., direct irradiation and indirect aqueous treatment), and practical feasibility (e.g., hand-held compact device and clinician-friendly operation). The objective of this comprehensive review is to summarize recent advances in the CAP-dominated skin therapy by broadly covering three aspects. We start with plasma optimisation of intact skin, detailing the effect of CAP on skin lipids, cells, histology, and blood circulation. We then conduct a clinically oriented and thorough dissection of CAP treatment of various skin diseases, focusing on the wound healing, inflammatory disorders, infectious conditions, parasitic infestations, cutaneous malignancies, and alopecia. Finally, we conclude with a brief analysis on the safety aspect of CAP treatment and a proposal on how to mitigate the potential risks. This comprehensive review endeavors to serve as a mini textbook for clinical dermatologists and a practical manual for plasma biotechnologists. Our collective goal is to consolidate plasma dermatology's lead in modern personalized medicine.
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http://dx.doi.org/10.3389/fonc.2022.918484DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314643PMC
July 2022
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