Publications by authors named "Yang Wang"

5,434 Publications

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Model-oriented Dose Optimization of Voriconazole in Critically Ill Children.

Antimicrob Agents Chemother 2021 Jun 21:AAC0049321. Epub 2021 Jun 21.

Department of Clinical Pharmacy, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan, China.

This study aimed to employ a population pharmacokinetic (PK) model to optimize the dosing regimen of voriconazole (VRC) in children with a critical illness. A total of 99 children aged from 0.44 to 13.58 years old were included in this study. The stability and predictive performance of the final model were evaluated by statistical and graphical methods. The optimal dosing regimen was proposed for children with different body weight, CYP2C19 phenotype, and co-administration with omeprazole. The PK of VRC was described by a two-compartment model with nonlinear Michaelis-Menten elimination. Body weight, CYP2C19 phenotype, and omeprazole were significant covariates on maximum velocity of elimination (V), which had an estimated typical value of 18.13 mg·h. Bayesian estimation suggested that dose-normalized concentration and total exposure (C/D, C/D, AUC/D) were significantly different between extensive metabolizers (EM) patients and poor metabolizer (PM) patients. To achieve the target concentration early, two loading doses of 9 mg·kg q12h were reliable for most children, whereas three loading doses of 6-7.5 mg·kg q8h were warranted for young children weighted ≤18kg (except PM patients). The maintenance doses decreased about 30-40% in PM patients than that in EM patients. For children aged < 2 years in EM, the maintenance dose could be as high as 9 mg·kg. The maintenance dose of VRC was supposed to decrease slightly when co-administration with omeprazole. A population PK model of intravenous VRC for critically ill children has been successful developed. It is necessary to adjust dosing regimens according to CYP2C19 genotype. The optimal dosing regimens have been recommended basing on the final model.
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http://dx.doi.org/10.1128/AAC.00493-21DOI Listing
June 2021

Serological Analysis Reveals an Imbalanced IgG Subclass Composition Associated with COVID-19 Disease Severity.

Cell Rep Med 2021 Jun 15:100329. Epub 2021 Jun 15.

Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, NY, 12208 USA.

COVID-19 is associated with a wide spectrum of disease presentation, ranging from asymptomatic infection to acute respiratory distress syndrome (ARDS). Paradoxically, a direct relationship has been suggested between COVID-19 disease severity and the levels of circulating SARS-CoV-2-specific antibodies, including virus neutralizing titers. A serological analysis of 536 convalescent healthcare workers reveal that SARS-CoV-2-specific and virus-neutralizing antibody levels are elevated in individuals that experience severe disease. The severity-associated increase in SARS-CoV-2-specific antibody is dominated by IgG, with an IgG subclass ratio skewed towards elevated receptor binding domain (RBD)- and S1-specific IgG3. In addition, individuals that experience severe disease show elevated SARS-CoV-2 specific antibody binding to the inflammatory receptor FcɣRIIIa. Based on these correlational studies, we propose that Spike-specific IgG subclass utilization may contribute to COVID-19 disease severity through potent Fc-mediated effector functions. These results may have significant implications for SARS-CoV-2 vaccine design and convalescent plasma therapy.
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http://dx.doi.org/10.1016/j.xcrm.2021.100329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205277PMC
June 2021

Stabilization of an At-Labeled Antibody with Sodium Ascorbate.

ACS Omega 2021 Jun 31;6(23):14887-14895. Epub 2021 May 31.

Department of Immune Medicine, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, 104-0045 Tokyo, Japan.

At, an α-particle emitter, has recently attracted attention for radioimmunotherapy of intractable cancers. However, our sodium dodecyl sulfate polyacrylamide gel electrophoresis and flow cytometry analyses revealed that At-labeled immunoconjugates are easily disrupted. Luminol assay revealed that reactive oxygen species generated from radiolysis of water caused the disruption of At-labeled immunoconjugates. To retain their functions, we explored methods to protect At-immunoconjugates from oxidation and enhance their stability. Among several other reducing agents, sodium ascorbate most safely and successfully protected At-labeled trastuzumab from oxidative stress and retained the stability of the At-labeled antibody and its cytotoxicity against antigen-expressing cells for several days.
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http://dx.doi.org/10.1021/acsomega.1c00684DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209801PMC
June 2021

METTL3 induces bone marrow mesenchymal stem cells osteogenic differentiation and migration through facilitating M1 macrophage differentiation.

Am J Transl Res 2021 15;13(5):4376-4388. Epub 2021 May 15.

Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University Harbin 150081, China.

Despite the crucial role of mA methyltransferase METTL3 in multiple diseases onset and progression, there are still lacking hard evidence proving that METTL3 could affect macrophage polarization in the stage of bone repair. Here, we aimed to explore the potential involvement of METTL3 in bone repair through modulating macrophage polarization and decipher the underlying cellular/molecular mechanisms. Here we treated RAW 264.7 cells and BM-derived primary macrophages (BMDM) with lipopolysaccharide (LPS) to induce M1 differentiation. METTL3 expression was upregulated in pro-inflammatory macrophages (M1) as compared with macrophages (M0). And overexpression of METTL3 promoted the expression of IL-6 and iNOS secretion by M1 macrophage. In the coculture condition, M1 macrophages with forced expression of METTL3 significantly enhanced migration ability of BMSCs, and also remarkably facilitated osteogenesis ability of BMSCs; the opposite was true when expression of METTL3 was knockdown. In addition, the mA-RIP microarray suggested that METTL3 silencing significantly reduce the mA modification of DUSP14, HDAC5 and Nfam1. Furthermore, the findings showed that expression of HADC5 was downregulated in M1 macrophages with METTL3 knockdown, while the DUSP14 expression had slight change and Nfam1 expression was very low. In contrast, METTL3 overexpression promoted HDAC5 expression, indicating that HDAC5 is the critical target gene of METTL3. Under such a theme, we proposed that METTL3 overexpression might be a new approach of replacement therapy for the treatment of bone repair.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205672PMC
May 2021

Cyclooxygenase-2 expressed hepatocellular carcinoma induces cytotoxic T lymphocytes exhaustion through M2 macrophage polarization.

Am J Transl Res 2021 15;13(5):4360-4375. Epub 2021 May 15.

Department of Hepatobiliary Surgery, Peking University People's Hospital, Beijing Key Laboratory of Basic Research on Liver Cirrhosis and Cancer, UMHS-PUHSC Joint Institute for Translational and Clinical Research Beijing, China.

Objective: Due to the tumor immune microenvironment (TIME) complexity and cancer heterogeneity, the clinical outcomes of hepatocellular carcinoma (HCC) are barely elicited from the conventional treatment options, even from the promising anti-cancer immunotherapy. As a suppressive TIME-related marker, the role played by cyclooxygenase-2 (COX-2) in HCC TIME, and its potential effects on anti-cancer T cell immune response remains unknown. In our study, to investigate the COX-2-dependent immune regulation pathway, we verified that the macrophages phenotypes were correlated to COX-2/PGE2 expressions among HCC patients. A multi-cellular co-culture platform containing HCC cells, macrophages, and T cells were established to mimic HCC TIME in vitro and in animal model. M2 macrophage polarization and activated CD8 T cells exhaustion were observed under high COX-2 levels in HCC cells, with further evaluation using CRISPR/Cas9-based PTGS2 knocking out and COX-2 blockade (celecoxib) treatment controls. PGE2, TGF-β, Granzyme B, and IFN-γ levels were testified by flow cytometry and ELISA to fully understand the mechanism of COX-2 suppressive effects on T cell-based anti-HCC responses. The activation of the TGF-β pathway evaluated by auto-western blot in T cells was confirmed which increased the level of phosphorylated Smad3, phosphorylated Samd2, and FoxP1, leading to T cell de-lymphotoxin. In conclusion, high COX-2-expressing HCC cell lines can induce anti-tumor abilities exhaustion in activated CD8 T cell through M2 TAMs polarization and TGF beta pathway. COX-2 inhibitors may reduce the inhibitory effect on CD8 T cells through regulating TAMs in TIME, thus enhance the T cell-based cytotoxicity and improve the prognosis of HCC patients.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205841PMC
May 2021

Retreatment With Flow Diverters and Coiling for Recurrent Aneurysms After Initial Endovascular Treatment: A Propensity Score-Matched Comparative Analysis.

Front Neurol 2021 3;12:625652. Epub 2021 Jun 3.

Department of Interventional Neuroradiology, Beijing Neurosurgical Institute and Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Flow diverters and conventional coiling are established modalities for the retreatment of intracranial recurrent aneurysms after initial endovascular treatment. We aimed to compare the efficacy of these techniques. We retrospectively analyzed data for patients with recurrent aneurysms after initial endovascular treatment retreated in our center with either a pipeline embolization device (PED) or conventional coil embolization from January 2012 to July 2020. We performed 1:2 propensity score matching (PSM) using the nearest neighbor method. We controlled for: initial treatment strategy, aneurysm size, neck diameter, symptom presentation, history of aneurysm rupture, age, sex, fusiform-dissecting aneurysm, bifurcation aneurysm, and aneurysm location. The clinical and morphological factors of all patients at initial treatment and the angiographic and clinical results at the second treatment were collected and compared between the propensity-matched pairs. A total of 105 intracranial aneurysms were identified; 18 patients (17.1%) were treated with a PED, and 87 (82.9%) were treated via conventional coil embolization. PSM resulted in 12 matched pairs (12 patients in the PED group and 24 in the coiling group). There was no significant difference of ischemic and hemorrhagic complications between the groups, the obliteration rate of branches covered by stent, or modified Rankin Scale scores at the last clinical follow-up. Importantly, the retreatment strategy in the PED group provided significantly different results vs. the coiling group ( < 0.001), with a lower recurrence rate (0.0 vs. 29.2%, respectively; = 0.037). However, the procedural failure rate and the parent artery stenosis were more frequently in PED group compared with coiling group (both were 16.7 vs. 0.0%; = 0.040). Endovascular retreatment for recurrent aneurysms after initial endovascular treatment might be safe and effective. Flow diverters might be associated with reduced risk of recanalization and an increased risk of procedural failure and mild parent artery stenosis.
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http://dx.doi.org/10.3389/fneur.2021.625652DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209336PMC
June 2021

Laser-Etched Stretchable Graphene-Polymer Composite Array for Sensitive Strain and Viscosity Sensors.

Nanomicro Lett 2019 Nov 16;11(1):99. Epub 2019 Nov 16.

CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Micro-nano Energy and Sensor, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, People's Republic of China.

The ability to control surface wettability and liquid spreading on textured surfaces is of interest for extensive applications. Soft materials have prominent advantages for producing the smart coatings with multiple functions for strain sensing. Here, we report a simple method to prepare flexible hydrophobic smart coatings using graphene-polymer films. Arrays of individual patterns in the films were created by laser engraving and controlled the contact angle of small drops by pinning the contact lines in a horizontal tensile range of 0-200%. By means of experiments and model, we demonstrate that the ductility of drops is relied on the height-to-spacing ratio of the individual pattern and the intrinsic contact angle. Moreover, the change of drop size was utilized to measure the applied strain and liquid viscosity, enabling a strain sensitivity as high as 1068 μm/%. The proposed laser-etched stretchable graphene-polymer composite has potential applications in DNA microarrays, biological assays, soft robots, and so on.
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http://dx.doi.org/10.1007/s40820-019-0333-6DOI Listing
November 2019

High-Power and Ultralong-Life Aqueous Zinc-Ion Hybrid Capacitors Based on Pseudocapacitive Charge Storage.

Nanomicro Lett 2019 Oct 31;11(1):94. Epub 2019 Oct 31.

Centre for Clean Energy Technology, Faculty of Science, University of Technology Sydney, Sydney, NSW, 2007, Australia.

Rechargeable aqueous zinc-ion hybrid capacitors and zinc-ion batteries are promising safe energy storage systems. In this study, amorphous RuO·HO for the first time was employed to achieve fast and ultralong-life Zn storage based on a pseudocapacitive storage mechanism. In the RuO·HO||Zn zinc-ion hybrid capacitors with Zn(CFSO) aqueous electrolyte, the RuO·HO cathode can reversibly store Zn in a voltage window of 0.4-1.6 V (vs. Zn/Zn), delivering a high discharge capacity of 122 mAh g. In particular, the zinc-ion hybrid capacitors can be rapidly charged/discharged within 36 s with a very high power density of 16.74 kW kg and a high energy density of 82 Wh kg. Besides, the zinc-ion hybrid capacitors demonstrate an ultralong cycle life (over 10,000 charge/discharge cycles). The kinetic analysis elucidates that the ultrafast Zn storage in the RuO·HO cathode originates from redox pseudocapacitive reactions. This work could greatly facilitate the development of high-power and safe electrochemical energy storage.
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http://dx.doi.org/10.1007/s40820-019-0328-3DOI Listing
October 2019

Diagnostic and prognostic value of tumor-infiltrating B cells in lymph node metastases of papillary thyroid carcinoma.

Virchows Arch 2021 Jun 19. Epub 2021 Jun 19.

Breast and Thyroid Surgical Department, Chongqing General Hospital, University of Chinese Academy of Sciences, Chongqing, China.

Lymph node metastases are strongly associated with unfavorable prognosis in papillary thyroid carcinoma (PTC) patients. However, there are few sensitive or specific indicators that can diagnose or predict lymph node metastases in PTC. The objective of our study was to identify reliable indicators for the diagnosis and prediction of lymph node metastases of PTC. The PTC data set was obtained from The Cancer Genome Atlas (TCGA) cohort. Information on tumor-infiltrating immune cells in PTC was acquired using single-sample gene set enrichment analysis (ssGSEA). Then, the progression-free survival (PFS) rates of PTC patients were evaluated by Kaplan-Meier curves. A tissue microarray including 58 normal thyroid tissues and 57 PTC tissues was processed for CD19 immunohistochemistry staining. Finally, evaluation of phenotype permutations was performed using gene set enrichment analysis (GSEA). There was an appreciable association between immune infiltration and lymph node metastases in PTC. Among those immune cells, B cells and cytotoxic cells showed significant predictive accuracy for lymph node metastases in PTC. Tumor-infiltrating B cells and NK cells were associated with favorable prognosis, while tumor-associated NK CD56 cells were correlated with poor prognosis in PTC patients. IHC analyses of PTC further confirmed a notably negative correlation between B cell infiltration and lymph node metastases in PTC. Additionally, mutations in BRAF, a dominant cause of tumor mutation burden (TMB), were positively correlated with reduced B cell infiltration and lymph node metastases in PTC. GSEA revealed that epithelial-mesenchymal transition, IL-6/JAK/STAT3 signaling, the inflammatory response, and TNF-α signaling via the NFκB pathway were remarkably suppressed pathways in patients with BRAF mutations. Tumor-associated lymphocytic infiltration, especially B cell infiltration, provides diagnostic and prognostic value for lymph node metastases in PTC.
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http://dx.doi.org/10.1007/s00428-021-03137-yDOI Listing
June 2021

Synthesis and biological evaluation of 1,2,4-oxadiazole core derivatives as potential neuroprotectants against acute Ischemic stroke.

Neurochem Int 2021 Jun 17:105103. Epub 2021 Jun 17.

Department of Medicinal chemistry, School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, 510006, P. R. China. Electronic address:

Here, we report the synthesis and neuroprotective capacity of 27 compounds with a bisphenol hydroxyl-substituted 1,2,4-triazole core or 1,2,4-oxadiazole core for stroke therapy. In vitro studies of the neuroprotective effects of compounds 1-27 on sodium nitroprusside (SNP)-induced apoptosis in PC12 cells indicate that compound 24 is the most effective compound conferring potent protection against oxidative injury. Compound 24 inhibits reactive oxygen species (ROS) accumulation and restores the mitochondrial membrane potential (MMP). Moreover, further analysis of the mechanism showed that compound 24 activates the antioxidant defence system by promoting the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and increasing the expression of haem oxygenase 1 (HO-1). An in vivo study was performed in a rat model of transient focal cerebral ischaemia generated by the intraluminal occlusion of the middle cerebral artery (MCAO). Compound 24 significantly reduced brain infarction and improved neurological function. Overall, compound 24 potentially represents a promising compound for the treatment of stroke.
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http://dx.doi.org/10.1016/j.neuint.2021.105103DOI Listing
June 2021

Sweat gland necrosis in systemic lupus erythematosus.

Rheumatology (Oxford) 2021 Jun;60(6):3031-3032

Department of Dermatology and Venerology, Peking University First Hospital, Beijing, China.

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http://dx.doi.org/10.1093/rheumatology/keaa707DOI Listing
June 2021

Exposure to heavy metal and antibiotic enriches antibiotic resistant genes on the tire particles in soil.

Sci Total Environ 2021 Jun 10;792:148417. Epub 2021 Jun 10.

CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 17 Chunhui Road, Yantai 264003, China.

The widespread occurrence of tire particles (TPs) in soils has attracted considerable attention due to their potential threats. The assemblage of bacteria and associated antibiotic resistant genes (ARGs) on TPs is yet largely unknown, especially under the stress of soil pollutants. In the present study, TPs were incubated in soils with or without the stress of heavy metal (Cu) or/and antibiotic (tetracycline), and bacterial community and ARG profile on TPs and in soils were explored using high-throughput sequencing and high-throughput quantitative PCR. Results indicated that bacterial community structure on TPs was significantly different from the surrounding soils, with a lower diversity, and significantly shifted by heavy metal and antibiotic exposure. Additionally, a diverse set of ARGs were detected on TPs, and their abundances were significantly increased under the stress of heavy metal and antibiotic, revealing a strong synergistic effect. Moreover, a good fit was observed for the correlation between bacterial community and ARG profile on TPs. Taken together, this study, for the first time, demonstrates that TPs can provide a novel niche for soil bacteria and soil resistome, and heavy metal and antibiotic exposure may potentially increase the abundance of ARGs on TPs, threatening soil ecosystems and human health.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148417DOI Listing
June 2021

Geniposide ameliorates chronic unpredictable mild stress induced depression-like behavior through inhibition of ceramide-PP2A signaling via the PI3K/Akt/GSK3β axis.

Psychopharmacology (Berl) 2021 Jun 17. Epub 2021 Jun 17.

Department of Neurosurgery & Neurocritical Care, Huashan Hospital Affiliated To Fudan University, 958 Jinguang Road, Minhang District, Shanghai, 200040, China.

Background: Depression is a severe mental disorder. Unfortunately, more than half of patients with major depression disorder cannot achieve remission after initial treatment with an antidepressant. Geniposide, a bioactive iridoid glycoside isolated from Gardenia jasminoides Ellis, can ameliorate depressive-like behaviors in mice. However, the underlying mechanism is still not very clear.

Methods: The pharmacological methods including ELISA, immunofluorescence, and Western blot were used to investigate the role of geniposide on chronic unpredictable mild stress (CUMS)-induced depression mice.

Results: In this study, we found that geniposide could inhibit CUMS-induced depressive-like behaviors in mice. Geniposide is able to reduce the levels of ceramide and lower the activity of acid sphingomyelinase (ASM) in hippocampus; besides, ASM inhibitor (amitriptyline) can decrease the concentration of ceramide and ameliorate depressive-like behaviors of mice. Moreover, geniposide can also alleviate CUMS-induced hippocampal neuronal apoptosis and increase the phosphorylated form of PI3K, Akt, and GSK3β. Additionally, PI3K inhibitor (LY294002) can also abolish the neuroprotective effect of geniposide on hippocampal neurons in vitro.

Conclusions: These results indicate that geniposide exert a potential antidepressant-like effect on CUMS mice, and its effect might be associated with activated PI3K/Akt/GSK3β signaling, reduced the level of ceramide and hippocampal neuron apoptosis.
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http://dx.doi.org/10.1007/s00213-021-05895-8DOI Listing
June 2021

Massive PD-L1 and CD8 double positive TILs characterize an immunosuppressive microenvironment with high mutational burden in lung cancer.

J Immunother Cancer 2021 Jun;9(6)

Department of Thoracic Surgery, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China

Background: Programmed cell death ligand 1 (PD-L1) expressed on tumor and immune cells are both associated with the response to programmed cell death 1 (PD-1) pathway blockade therapy. Here, we examine the role of CD8+PD-L1+ tumor-infiltrating lymphocyte (Tils) in the tumor microenvironment of non-small cell lung cancer (NSCLC).

Methods: Tumor tissue samples of a total of 378 patients from two NSCLC cohorts were collected retrospectively. Tumor genetic variations were measured by targeted next-generation sequencing of 543 oncogenes. Tils were assessed by multiplex immunohistochemistry assay. Correlations among Tils, tumor genetic variations, and clinicopathological characteristics were analyzed.

Results: The levels of CD8+PD-L1+ Tils varied in NSCLC tumor tissues. Tumor samples with high CD8+PD-L1+ Tils had higher levels of CD8+ Tils, CD68+ macrophages, PD-L1+ tumor cells, PD-1+ Tils, and CD163+ M2-type macrophages, and also had a higher tumor mutation burden, all of which collectively constituted a typically hot but immunosuppressive tumor microenvironment. Therefore, in a non-immunotherapy cohort, we observed that the higher the CD8+PD-L1+ Tils level in the tumor tissue, the worse the prognosis (progression-free survival; cohort A, stage I-II tumor; p=0.005). Contrarily, in an immunotherapy cohort, where the immune suppression was blocked by anti-PD-1 treatment, the higher the CD8+PD-L1+ Tils level, the better the response to the anti-PD-1 treatment (complete response/partial response vs stable disease/progressive disease; cohort B; p=0.0337).

Conclusions: CD8+PD-L1+ Tils may be an indicator of the hot but immunosuppressive tumor microenvironment which is related to a high tumor mutation burden. PD-1 pathway blockade therapy can help to mitigate this immunosuppression and obtain better curative effects.
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http://dx.doi.org/10.1136/jitc-2021-002356DOI Listing
June 2021

Designed new magnetic functional three-dimensional hierarchical flowerlike micro-nano structure of [email protected]/NiCo-layered double oxides for highly efficient co-adsorption of multiple environmental pollutants.

Authors:
Yang Wang Lei Zhang

J Colloid Interface Sci 2021 Jun 6;602:469-479. Epub 2021 Jun 6.

College of Chemistry, Liaoning University, Shenyang 110036, PR China. Electronic address:

In order to eliminate multiple coexisting pollutants in environmental wastewater, a magnetic three-dimensional hierarchical porous flower-like N, Co-doped graphitic carbon nano-polyhedra decorated NiCo-layered double oxides ([email protected]/NiCo-LDOs) adsorption material was synthesized, which consisted of two-dimensional LDOs nanosheets with functionalized surfaces (N, Co-doped graphitic carbon loaded on both sides of NiCo-LDOs nanosheets). The adsorption properties of [email protected]/NiCo-LDOs for five types of typical pollutants (cationic dyes: rhodamine b, methylene blue; pesticides: ethofenprox, bifenthrin; anionic dyes: methyl orange, congo red; inorganic cations: Cr, Cd, Pb, Zn, inorganic anions: CrO, AsO) were investigated systematically in single and coexisting systems. Combined with the results of FTIR and zeta potential, the adsorption mechanism was discussed. By virtue of its hierarchical porous architecture and the combined effect of functionalized surfaces and LODs supporter, the as-prepared [email protected]/NiCo-LDOs demonstrates excellent adsorption performance towards five types of typical pollutants with fast adsorption rate, high adsorption capacity and good co-adsorption performance. More importantly, the [email protected]/NiCo-LDOs showed satisfactory removal efficiency, stability and reusability in model wastewater. The broad-spectrum, rapid, easily separable, and reusable adsorption properties make [email protected]/NiCo-LDOs promising for highly efficient wastewater treatments. This work also provides a feasible way for the preparation of adsorption materials for the treatment of complex wastewater systems.
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http://dx.doi.org/10.1016/j.jcis.2021.06.023DOI Listing
June 2021

Impact of Amine Additives on Perovskite Precursor Aging: A Case Study of Light-Emitting Diodes.

J Phys Chem Lett 2021 Jun 17:5836-5843. Epub 2021 Jun 17.

Department of Physics Chemistry and Biology (IFM), Linköping University, Linköping SE-58183, Sweden.

Amines are widely employed as additives for improving the performance of metal halide perovskite optoelectronic devices. However, amines are well-known for their high chemical reactivity, the impact of which has yet to receive enough attention from the perovskite light-emitting diode community. Here, by investigating an unusual positive aging effect of CHNHI/CsI/PbI precursor solutions as an example, we reveal that amines gradually undergo N-formylation in perovskite precursors over time. This reaction is initialized by hydrolysis of dimethylformamide in the acidic chemical environment. Further investigations suggest that the reaction products collectively impact perovskite crystallization and eventually lead to significantly enhanced external quantum efficiency values, increasing from ∼2% for fresh solutions to ≳12% for aged ones. While this case study provides a positive aging effect, a negative aging effect is possible in other perovksite systems. Our findings pave the way for more reliable and reproducible device fabrication and call for further attention to underlying chemical reactions within the perovskite inks once amine additives are included.
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http://dx.doi.org/10.1021/acs.jpclett.1c01349DOI Listing
June 2021

Towards High-Energy and Anti-Self-Discharge Zn-Ion Hybrid Supercapacitors with New Understanding of the Electrochemistry.

Nanomicro Lett 2021 Mar 18;13(1):95. Epub 2021 Mar 18.

College of Chemistry and Materials Science, Jinan University, Guangzhou, 511443, People's Republic of China.

Aqueous Zn-ion hybrid supercapacitors (ZHSs) are increasingly being studied as a novel electrochemical energy storage system with prominent electrochemical performance, high safety and low cost. Herein, high-energy and anti-self-discharge ZHSs are realized based on the fibrous carbon cathodes with hierarchically porous surface and O/N heteroatom functional groups. Hierarchically porous surface of the fabricated free-standing fibrous carbon cathodes not only provides abundant active sites for divalent ion storage, but also optimizes ion transport kinetics. Consequently, the cathodes show a high gravimetric capacity of 156 mAh g, superior rate capability (79 mAh g with a very short charge/discharge time of 14 s) and exceptional cycling stability. Meanwhile, hierarchical pore structure and suitable surface functional groups of the cathodes endow ZHSs with a high energy density of 127 Wh kg, a high power density of 15.3 kW kg and good anti-self-discharge performance. Mechanism investigation reveals that ZHS electrochemistry involves cation adsorption/desorption and ZnSO(OH)·5HO formation/dissolution at low voltage and anion adsorption/desorption at high voltage on carbon cathodes. The roles of these reactions in energy storage of ZHSs are elucidated. This work not only paves a way for high-performance cathode materials of ZHSs, but also provides a deeper understanding of ZHS electrochemistry.
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http://dx.doi.org/10.1007/s40820-021-00625-3DOI Listing
March 2021

Thermochromic Silks for Temperature Management and Dynamic Textile Displays.

Nanomicro Lett 2021 Feb 14;13(1):72. Epub 2021 Feb 14.

School of Physical Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Shanghai, 201210, People's Republic of China.

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http://dx.doi.org/10.1007/s40820-021-00591-wDOI Listing
February 2021

Pathogen identification of root-knot nematode disease in Yulong, China.

J Nematol 2021 12;53. Epub 2021 Jun 12.

School of Landscape and Horticulture, Southwest Forestry University, Yunnan, Kunming 650233, China.

In September 2020, samples of galled roots with rhizosphere soil were collected from declining in Yulong County, China. The pathogenic nematodes were identified by observing morphological characteristics of females, second-stage juveniles and perineal pattern, sequence alignments, and specific amplification of sequence characterized amplified region (SCAR). The results showed that the perineal pattern of this nematode was round or oval, the dorsal arch was moderately high or low, one side or both of the lateral field extended to form a wing shape, the tail region had punctations, and the morphological characteristics and morphometric values of second-stage juveniles and females were similar to those of . The ITS region fragment of this nematode were highly similar to those of in NCBI database, with a similarity of over 99.35%. Using the SCAR specific primers, a specific band with an expected size of approximately 440 bp was amplified from this nematode. Morphological and molecular identification supports the nematode species found on Gentiana macrophylla as . This is the first report of this regulated root-knot nematode on in China.
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http://dx.doi.org/10.21307/jofnem-2021-056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204197PMC
June 2021

The small GTP-binding protein TaRop10 interacts with TaTrxh9 and functions as a negative regulator of wheat resistance against the stripe rust.

Plant Sci 2021 Aug 14;309:110937. Epub 2021 May 14.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address:

Small GTP-binding proteins, also known as ROPs (Rho of Plants), are a subfamily of the Ras superfamily of signaling G-proteins and are required for numerous signaling processes, ranging from growth and development to biotic and abiotic signaling. In this study, we cloned and characterized wheat TaRop10, a homolog of Arabidopsis ROP10 and member of the class II ROP, and uncovered a role for TaRop10 in wheat response to Puccinia striiformis f. sp. tritici (Pst). TaRop10 was downregulated by actin depolymerization and was observed to be differentially induced by abiotic stress and the perception of plant hormones. A combination of yeast two-hybrid and bimolecular fluorescence complementation assays revealed that TaRop10 interacted with a h-type thioredoxin (TaTrxh9). Knocking-down of TaRop10 and TaTrxh9 was performed using the BSMV-VIGS (barley stripe mosaic virus-based virus-induced gene silencing) technique and revealed that TaRop10 and TaTrxh9 play a role in the negative regulation of defense signaling in response to Pst infection. In total, the data presented herein further illuminate our understanding of how intact plant cells accommodate fungal infection structures, and furthermore, support the function of TaRop10 and TaTrxh9 in negative modulation of defense signaling in response to stripe rust infection.
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http://dx.doi.org/10.1016/j.plantsci.2021.110937DOI Listing
August 2021

Gene expression profile analysis and target gene discovery of Mycobacterium tuberculosis biofilm.

Appl Microbiol Biotechnol 2021 Jun 14. Epub 2021 Jun 14.

Key Laboratory for Zoonoses Research, Ministry of Education, Institute of Zoonosis, Department of Infectious Diseases, First Hospital of Jilin University, College of Veterinary Medicine, Jilin University, Changchun, 130062, China.

Tuberculosis (TB) caused by Mycobacterium tuberculosis (M. tuberculosis) is a fatal infectious disease to human health, and the drug tolerance and immune evasion of M. tuberculosis were reported to be related to its biofilm formation; however, the difficulty of M. tuberculosis biofilm culture and its unknown global mechanism impede its further research. Here, we developed a modified in vitro M. tuberculosis biofilm model with shorter culture time. Then we used Illumina RNA-seq technology to determine the global gene expression profile of M. tuberculosis H37Rv biofilms. Over 437 genes are expressed at significantly different levels in biofilm cells than in planktonic cells; among them, 153 were downregulated and 284 were upregulated. Go enrichment analysis and KEGG pathway analysis showed that genes involved in biosynthesis and metabolism of sulfur metabolism, steroid degradation, atrazine degradation, mammalian cell entry protein complex, etc. are involved in M. tuberculosis biofilm cells. Especially, ATP-binding cassette (ABC) transporters Rv1217c and Rv1218c were significantly upregulated in biofilm, whereas efflux pump inhibitors (EPIs) piperine and 1-(1-naphthylmethyl)-piperazine (NMP) inhibited biofilm formation and the expression of the Rv1217c and Rv1218c genes in a concentration-dependent manner, respectively, indicating Rv1217c and Rv1218c are potential target genes of M. tuberculosis biofilm. This study is the first RNA-Seq-based transcriptome profiling of M. tuberculosis biofilms and provides insights into a potential strategy for M. tuberculosis biofilm inhibition. KEY POINTS: • Characterize M. tuberculosis transcriptomes in biofilm cells by RNA-seq. • Inhibit the expression of Rv1217c and Rv1218c repressed biofilm formation.
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http://dx.doi.org/10.1007/s00253-021-11361-4DOI Listing
June 2021

Rapid detection of human origin colistin-resistance genes mcr-1, mcr-3, mcr-8, mcr-10 in clinical fecal samples.

Arch Microbiol 2021 Jun 14. Epub 2021 Jun 14.

Key Laboratory of Molecular Epidemiology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Guangdong Province, No. 8 Longyuan Road, Nanshan district, Shenzhen City, 518055, People's Republic of China.

Plasmid-mediated colistin-resistance genes have been reported in human origin clinical samples worldwide which raises its threats to human infections. Notably, mcr-1, mcr-3, mcr-8, and mcr-10 have been reported isolated directly from clinical samples which creates more seriously threaten to human health than other mcr gene types. A multiplex polymerase chain reaction (Multi-PCR) protocol was developed to detect and genotype mobile colistin-resistance genes (mcr-1, mcr-3, mcr-8, mcr-10) in Enterobacteria for clinical laboratory purposes. We first designed four pairs of new primers for the amplification of mcr-1, mcr-3, mcr-8, and mcr-10 gene respectively to achieve stepwise separation of amplicons between 216 and 241 bp, and complete this Multi-PCR system with the assistance of another pair of universal primer. Among which the forward primers for mcr-8 and mcr-10 amplicons were identical. The protocol was validated by testing 11 clinical isolates of Escherichia coli and 3 clinical isolates of Klebsiella from human origin, each well characterized and prospectively validated. The Multi-PCR assay showed full concordance with whole-genome sequence data and displayed higher sensitivity and 100% specificity. The assay could detect all variants of the various mcr alleles described. The Multi-PCR assay successfully genotyped of mcr alleles described in one test.
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http://dx.doi.org/10.1007/s00203-021-02407-2DOI Listing
June 2021

A Patient-Controlled Intravenous Analgesia With Tramadol Ameliorates Postpartum Depression in High-Risk Woman After Cesarean Section: A Randomized Controlled Trial.

Front Med (Lausanne) 2021 27;8:679159. Epub 2021 May 27.

Department of Anesthesiology, Second Affiliated Hospital of Army Medical University, People's Liberation Army of China (PLA), Chongqing, China.

Postpartum depression (PPD) is a severe psychiatric disorder. Its risk is associated with the cesarean section (CS). Currently, there are few early intervention strategies for these women with PPD who underwent CS. This was a parallel-group randomized controlled trial of singleton pregnant women who underwent elective CS in a tertiary referral hospital in China from October, 2017 to September, 2019. After operation, patients received randomly tramadol patient-controlled intravenous analgesia (PCIA; 4 mg/ml; TRA group), hydromorphone PCIA (0.04 mg/ml; HYD group), or ropivacaine patient-controlled epidural analgesia (PCEA; 1.5 mg/ml; ROP group) for 48 h in a 1:1:1 ratio. Total blinding during hospitalization was not feasible due to differences between the PCEA and PCIA treatments. All investigators who performed the follow-up were blinded to the group assignment. A total of 1,230 patients were enrolled for eligibility. Intention-to-treat analysis showed reduced incidence of PPD in the TRA group ( = 27 [6.6%]) than that in the HYD (10.2%, OR 1.62, 95% CI 0.98~2.68; = 0.059) and ROP groups (10.5%, OR 1.66, 95% CI 1.01~2.75; = 0.046) at 4 weeks post-operation, however, the difference was not statistically significant (Bonferroni corrected = 0.118, = 0.098, respectively). Subgroup analysis in high-risk women (preoperative Edinburgh Postpartum Depression Scale [EPDS] ≥10) showed a significantly lower incidence of PPD in the TRA group (16.5%) than in the HYD (32.6%) and ROP groups (30.9%) (Bonferroni corrected = 0.022 and = 0.038, respectively). The per-protocol analysis yielded similar results. Reported adverse events (AEs) were mostly mild. None of the women or infant discontinued treatment due to AEs. Tramadol PCIA after CS in high-risk women can help to reduce the risk of PPD at 4 weeks after elective CS. https://clinicaltrials.gov/ct2/show/NCT03309163?term=ETPPD&draw=2&rank=1; ClinicalTrials.gov (NCT03309163).
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http://dx.doi.org/10.3389/fmed.2021.679159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191376PMC
May 2021

Genotypic Males Play an Important Role in the Creation of Genetic Diversity in Gynogenetic Gibel Carp.

Front Genet 2021 28;12:691923. Epub 2021 May 28.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Hubei Hongshan Laboratory, Chinese Academy of Sciences, Wuhan, China.

Unisexual lineages are commonly considered to be short-lived in the evolutionary process as accumulation of deleterious mutations stated by Muller's ratchet. However, the gynogenetic hexaploid gibel carp () with existence over 0.5 million years has wider ecological distribution and higher genetic diversity than its sexual progenitors, which provides an ideal model to investigate the underlying mechanisms on countering Muller's ratchet in unisexual taxa. Unlike other unisexual lineages, the wild populations of gibel carp contain rare and variable proportions of males (1-26%), which are determined via two strategies including genotypic sex determination and temperature-dependent sex determination. Here, we used a maternal gibel carp from strain F to be mated with a genotypic male from strain A, a temperature-dependent male from strain A, and a male from another species common carp (), respectively. When the maternal individual was mated with the genotypic male, a variant of gynogenesis was initiated, along with male occurrence, accumulation of microchromosomes, and creation of genetic diversity in the offspring. When the maternal individual was mated with the temperature-dependent male and common carp, typical gynogenesis was initiated that all the offspring showed the same genetic information as the maternal individual. Subsequently, we found out that the genotypic male nucleus swelled and contacted with the female nucleus after fertilization although it was extruded from the female nucleus eventually, which might be associated with the genetic variation in the offspring. These results reveal that genotypic males play an important role in the creation of genetic diversity in gynogenetic gibel carp, which provides insights into the evolution of unisexual reproduction.
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http://dx.doi.org/10.3389/fgene.2021.691923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194356PMC
May 2021

Do Experiences Studying Abroad Promote Dialectical Thinking? Empirical Evidence From Chinese International Students.

Front Psychol 2021 28;12:595935. Epub 2021 May 28.

Department of Psychology, Tsinghua University, Beijing, China.

Our current work seeks to provide direct empirical evidence on whether Chinese international students' experiences studying abroad promote dialectical thinking. We collected behavioral data from 258 Chinese international students studying in multiple regions. We found that there was a main effect among the four conditions (i.e., studying abroad, exposure to foreign culture, hometown, and typical day). More specifically, when primed with studying abroad or typical day (relative to hometown culture), participants were more likely to show tolerance for contradiction by deeming both sides of contradictory scientific statements as convincing and rating them more favorably. Therefore, it is plausible that Chinese international students' experiences studying abroad promote their dialectical thinking. More work is needed to further this line of research by (1) extending these effects with other measures of dialectical thinking such as perception of interconnectedness and prediction of change, (2) adopting differing paradigms to provide more robust findings, and (3) probing the underlying processes as to why experiences studying abroad promote dialectical thinking.
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http://dx.doi.org/10.3389/fpsyg.2021.595935DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195590PMC
May 2021

First report of Meloidogyne arenaria infecting Maidong (Ophiopogon japonicus) in China.

Plant Dis 2021 Jun 11. Epub 2021 Jun 11.

Southwest Foresttry UniversityKunming, Yunnan, China, 650224;

Maidong (Ophiopogon japonicus) is a perennial evergreen plant of the Asparagaceae, occurring mainly in China, Japan, Vietnam, and India. It grows in the damp place on the hillside below 2000 meters above sea level, under the forest or beside the stream;It has been widely cultivated in the Sichuan ofhina for medicinal uses; and it is included in the Chinese Pharmacopoeia. During April 2019, Maidong plants exhibiting symptoms of stunting, leaf wilting, and multiple galls in the roots associated with root-knot nematode (Meloidogyne sp.) were detected in a commercial field in near the city of Mianyang (N105°42', E30°93'), Sichuan, China. The second-stage juveniles (J2) were collected from the soil in the root zone, and adult females were dissected from roots. Population densities of J2 ranged from 190 to 255 per 100 cm3. Subsequently, individual females (n=20) were extracted from root samples and submitted to Meloidogyne species identification by perineal pattern morphological analysis (n=20), and morphometric measurements of second stage juveniles (J2) (n = 20). The J2 showed the following morphometric characters:body length = 475.5 ± 24.2 µm, tail length = 55.2 ± 6.43µm, stylet length = 12.4 ± 1.56 µm and distance from dorsal esophageal gland opening to the stylet knot (DGO) = 2.97 ± 0.44 μm; perineal patterns of females showed a low dorsal arch, with lateral field marked by forked and broken striae, no punctate markings between anus and tail terminus were observed. These morphological characteristics are consistent with Meloidogyne arenaria (Neves et al. 2016). In addition, to confirm species identification, DNA was extracted from females (Blok, et al. 1997) and D2/D3 fragments of the 28S rRNA was amplified using the universal primers D2A/D3B. The DNA fragment obtained showed a 754 bp length (GenBank accession no. MW965614) that was sequenced and analyzed, sequences were 99.8% identical to the MH359158, KX151138 and EU364889 M. arenaria sequences. Furthermore, species-specific SCAR primers Far/Rar were used as described by Zijlstra et al. 2000. The PCR produced approximately 420 bp sequences, which was identical to that previously reported for M. arenaria (Zijlstra et al. 2000). Morphological and molecular characterization supports the identification of the isolate found on Ophiopogon japonicus as M. arenaria. To verify the nematode pathogenicity on Maidong plants, Maidong seed were planted in 20-cm diameter, 10-cm deep plastic pots containing 1000 cm3 sterilized soil and infested with 2000 M. arenaria J2 per seedling, using a sterilized micropipette. Plants were maintained at 20-25°C in a greenhouse. Control plants received sterile water, and the pathogenicity test was repeated three times. After 60 days, all inoculated plants showed reduced growth compared with control. The symptoms were similar to those observed in the field, a large number of galls (38.5 ± 2.4) and egg masses (18.5 ± 0.2) were found on each root system. Maidong was considered a good host for M. arenaria in Mianyang. M. arenaria is one of the most important plant parasitic nematode with a wide geographic distribution and causes great losses in many crops around the world (Perry et al. 2009). Through investigation, this is the first report worldwide of M. arenaria infecting Ophiopogon japonicus.
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http://dx.doi.org/10.1094/PDIS-03-21-0584-PDNDOI Listing
June 2021

Rabies Virus-Induced Autophagy Is Dependent on Viral Load in BV2 Cells.

Front Microbiol 2021 25;12:595678. Epub 2021 May 25.

College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

An increasing number of studies are showing that autophagy plays a vital role in viral replication and escape. Rabies virus (RABV), a typical neurotropic virus, has been proven to induce autophagy in neurons. However, there are no reports indicating that RABV can cause autophagy in other cells of the central nervous system. Thus, we aimed to explore the relationship between autophagy and RABV infection in BV2 cells in this study. Results of viral growth curves showed that the titers of microglial BV2 cells infected with RABV peaked at 12 hours post-infection (hpi) and then decreased continuously over time. However, it was found that the viral genome RNA and structural proteins can express normally in BV2 cells. In addition, Western blotting indicated that RABV infection increased LC3-II and p62 expression in BV2 cells. LC3 punctate increased with RABV infection in BV2 cells after the transfection of fluorescent protein-tagged LC3 plasmids. Moreover, autophagy cargo protein further accumulated with RABV infection in Bafilomycin A1-treated cells. Subsequently, RABV infection inhibited the fusion of autophagosomes with lysosomes by using a tandem fluorescent marker. Furthermore, a higher multiplicity of infection induced stronger autophagy. Thus, RABV can induce autophagy in BV2 cells, and the autophagy is positively associated with the viral load.
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http://dx.doi.org/10.3389/fmicb.2021.595678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186530PMC
May 2021

Improving NKCC1 Function Increases the Excitability of DRG Neurons Exacerbating Pain Induced After TRPV1 Activation of Primary Sensory Neurons.

Front Cell Neurosci 2021 25;15:665596. Epub 2021 May 25.

The Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Department of Physiology, Shihezi University Medical College, Shihezi, China.

Our aim was to investigate the effects of the protein expression and the function of sodium, potassium, and chloride co-transporter (NKCC1) in the dorsal root ganglion (DRG) after activation of transient receptor potential vanilloid 1 receptor (TRPV1) in capsaicin-induced acute inflammatory pain and the possible mechanism of action. Male Sprague-Dawley rats were randomly divided into control, capsaicin, and inhibitor groups. The expression and distribution of TRPV1 and NKCC1 in rat DRG were observed by immunofluorescence. Thermal radiation and acetone test were used to detect the pain threshold of heat and cold noxious stimulation in each group. The expressions of NKCC1 mRNA, NKCC1 protein, and p-NKCC1 in the DRG were detected by PCR and western blotting (WB). Patch clamp and chloride fluorescent probe were used to observe the changes of GABA activation current and intracellular chloride concentration. After intrathecal injection of protein kinase C (PKC) inhibitor (GF109203X) or MEK/extracellular signal-regulated kinase (ERK) inhibitor (U0126), the behavioral changes and the expression of NKCC1 and p-ERK protein in L DRG were observed. TRPV1 and NKCC1 were co-expressed in the DRG. Compared with the control group, the immunofluorescence intensity of NKCC1 and p-NKCC1 in the capsaicin group was significantly higher, and the expression of NKCC1 in the nuclear membrane was significantly higher than that in the control group. The expression of NKCC1 mRNA and protein of NKCC1 and p-NKCC1 in the capsaicin group were higher than those in the control group. After capsaicin injection, GF109203X inhibited the protein expression of NKCC1 and p-ERK, while U0126 inhibited the protein expression of NKCC1. In the capsaicin group, paw withdrawal thermal latency (WTL) was decreased, while cold withdrawal latency (CWL) was prolonged. Bumetanide, GF109203X, or U0126 could reverse the effect. GABA activation current significantly increased in the DRG cells of the capsaicin group, which could be reversed by bumetanide. The concentration of chloride in the DRG cells of the capsaicin group increased, but decreased after bumetanide, GF109203X, and U0126 were administered. Activation of TRPV1 by exogenous agonists can increase the expression and function of NKCC1 protein in DRG, which is mediated by activation of PKC/p-ERK signaling pathway. These results suggest that DRG NKCC1 may participate in the inflammatory pain induced by TRPV1.
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http://dx.doi.org/10.3389/fncel.2021.665596DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185156PMC
May 2021

GPC3 affects the prognosis of lung adenocarcinoma and lung squamous cell carcinoma.

BMC Pulm Med 2021 Jun 10;21(1):199. Epub 2021 Jun 10.

Molecular Oncology Department of Cancer Research Institution, The First Hospital of China Medical University, Nanjingbei Street, Heping District, Shenyang, 110001, Liaoning Province, China.

Background: Glypican 3 (GPC3) is a heparin sulphate proteoglycan whose expression is associated with several malignancies. However, its expression in non-small-cell lung carcinoma (NSCLC) is limited and ambiguous. This study aimed to comprehensively evaluate the expression of GPC3 in NSCLC and develop a risk-score model for predicting the prognosis of NSCLC.

Methods: The gene expression profiles of lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) were downloaded from the UCSC Xena database. Using the limma package, the differentially expressed genes (DEGs) between different comparison groups were analysed and the differential expression of GPC3 was calculated. A functional enrichment analysis was conducted for GPC3-associated genes using the DAVID tool. For the GPC3-associated genes shared by the four comparison groups, a protein-protein interaction network was built using the Cytoscape software. After conducting a survival analysis and a Cox regression analysis, the genes found to be significantly correlated with prognosis were selected to construct a risk-score model. Besides, the gene and protein levels of GPC3 were examined by quantitative reverse transcriptase-PCR (qRT-PCR) and immunohistochemistry (IHC) in LUSC tissues and paracancer tissues.

Results: The differential expression of GPC3 was significant (adjusted P < 0.05) in the NSCLC vs. normal, LUAD vs. normal, LUSC versus normal, and LUAD versus. LUSC comparison groups. GPC3 directly interacted with SERPINA1, MFI2, and FOXM1. Moreover, GPC3 expression was significantly correlated with pathologic N, pathologic T, gender, and tumour stage in LUAD samples. Finally, the risk-score model (involving MFI2, FOXM1, and GPC3) for LUAD and that (involving SERPINA1 and FOXM1) for LUSC were established separately. The qRT-PCR result showed that GPC3 expression was much higher in the LUSC tissues than that in the normal group. The IHC results further showed that GPC3 is highly expressed in LUSC tissues, but low in paracancer tissues.

Conclusion: The three-gene risk-score model for LUAD and the two-gene risk-score model for LUSC might be valuable in improving the prognosis of these carcinomas.
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http://dx.doi.org/10.1186/s12890-021-01549-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194200PMC
June 2021

Optimization of plasmonic metal structures for improving the hydrogen production efficiency of metal-organic frameworks.

Nanoscale 2021 Jun 10. Epub 2021 Jun 10.

Department of Chemistry, School of Science, Beijing Jiaotong University, Beijing 100044, People's Republic of China.

The introduction of plasmonic metals of gold (Au) nanoparticles onto metal-organic frameworks (MOFs) has been testified as an efficient approach to boost the H2 evolution ability because the excited Au particles can generate hot electrons that can then be injected into MOFs to inhibit the recombination of charges. Generally, Au particles possess two modes of polarization, which are transverse and longitudinal polarizations. However, which of the two modes is more efficient remains unclear and is yet to be disclosed. Herein, we report a strategy of finely controlling the transverse and longitudinal polarizations by gradually reducing the length, without changing the width of Au rods, and then combining with MOF [email protected] to construct [email protected]/Au Schottky junctions, for investigating the polarization mode effects. The results indicate that with the reduction in the length of Au rods from 67 nm to 38 nm, the transverse polarization will be increased, which can lead to a significant enhancement in the electron-hole separation efficiency and H2 generation activity. When Au is in spherical shape, the transverse polarization effect reaches the highest level, thereby achieving the highest H2 production rate of 265.1 μmol g-1 h, which is 1.6 times more than that of 67 nm Au rods. The enhancement can be attributed to the significant accelerated electron transfer rate induced by transverse polarization and evidenced by ESR analysis. The results indicate that the transverse polarization is more efficient for hot electron injection and highlight that the Au sphere is a more appropriate candidate for producing the maximum SPR effect during photocatalysis.
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http://dx.doi.org/10.1039/d1nr01197aDOI Listing
June 2021