Publications by authors named "Yang Qiu"

355 Publications

Multi-omic profiling of plasma reveals molecular alterations in children with COVID-19.

Theranostics 2021 6;11(16):8008-8026. Epub 2021 Jul 6.

Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510120, China.

Children usually develop less severe symptoms responding to Coronavirus Disease 2019 (COVID-19) than adults. However, little is known about the molecular alterations and pathogenesis of COVID-19 in children. We conducted plasma proteomic and metabolomic profilings of the blood samples of a cohort containing 18 COVID-19-children with mild symptoms and 12 healthy children, which were enrolled from hospital admissions and outpatients, respectively. Statistical analyses were performed to identify molecules specifically altered in COVID-19-children. We also developed a machine learning-based pipeline named inference of biomolecular combinations with minimal bias (iBM) to prioritize proteins and metabolites strongly altered in COVID-19-children, and experimentally validated the predictions. By comparing to the multi-omic data in adults, we identified 44 proteins and 249 metabolites differentially altered in COVID-19-children against healthy children or COVID-19-adults. Further analyses demonstrated that both deteriorative immune response/inflammation processes and protective antioxidant or anti-inflammatory processes were markedly induced in COVID-19-children. Using iBM, we prioritized two combinations that contained 5 proteins and 5 metabolites, respectively, each exhibiting a total area under curve (AUC) value of 100% to accurately distinguish COVID-19-children from healthy children or COVID-19-adults. Further experiments validated that all the 5 proteins were up-regulated upon coronavirus infection. Interestingly, we found that the prioritized metabolites inhibited the expression of pro-inflammatory factors, and two of them, methylmalonic acid (MMA) and mannitol, also suppressed coronaviral replication, implying a protective role of these metabolites in COVID-19-children. The finding of a strong antagonism of deteriorative and protective effects provided new insights on the mechanism and pathogenesis of COVID-19 in children that mostly underwent mild symptoms. The identified metabolites strongly altered in COVID-19-children could serve as potential therapeutic agents of COVID-19.
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http://dx.doi.org/10.7150/thno.61832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315065PMC
July 2021

MiR-1299 functions as a tumor suppressor to inhibit the proliferation and metastasis of gastric cancer by targeting ARF6.

Genes Genomics 2021 Jul 27. Epub 2021 Jul 27.

Department of Breast Surgery, Nanchang Third Hospital, Jiangxi, 330002, Nanchang, China.

Background: MiRNAs belong to non-coding RNAs that are involved in cancer development. Acting as a mediator, they could regulate the expression level of numerous gens. However, the expression and function of miR-1299 in gastric cancer (GC) are not clear.

Objective: To explore the role of miR-1299 in the process of GC.

Methods: We detected the levels of miR-1299 in clinical samples of GC and investigated the role of miR-1299 in the regulation of the GC cells proliferation, apoptosis and metastasis. Luciferase reporter assay was employed to verify the target of miR-1299. Additionally, the proliferation, apoptosis and metastasis of AGS and SGC7901 cells were analyzed after the overexpression of miR-1299.

Results: Our study showed the expression of miR-1299 was decreased in GC tissues and cell lines. It indicated that the cell proliferation, migration and invasion was inhibited, while the cell apoptosis was promoted by the over-expressed miR-1299. Also, we found that miR-1299 could directly target the 3'-untranslated region (3'UTR) of ARF6 genes. In addition, rescue assay demonstrated that miR-1299 overexpression promoted the cell apoptosis and inhibited cell growth, which could be attenuated by the overexpression of ARF6.

Conclusions: These findings indicate that miR-1299 regulates cell progression in GC by targeting ARF6 genes, and suggest that miR-1299 may be a tumor suppressor in the GC progression.
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http://dx.doi.org/10.1007/s13258-021-01124-wDOI Listing
July 2021

[Effects of Water and Fertilization Management on CH and NO Emissions in Double-rice Paddy Fields in Tropical Regions].

Huan Jing Ke Xue 2021 Jul;42(7):3458-3471

College of Tropical Crops, Hainan University, Haikou 570228, China.

Paddy soils are widely considered a main source of methane (CH) and nitrous oxide (NO). Comprehensively evaluating CH and NO emissions from double-rice systems in tropical regions with different water irrigation and fertilizer applications is of great significance for addressing greenhouse gas emissions from such systems in China. In this study, eight treatments were evaluated:conventional irrigation-PK fertilizer (D-PK), conventional irrigation-NPK fertilizer (D-NPK), conventional irrigation-NPK+organic fertilizer (D-NPK+M), conventional irrigation-organic fertilizer (D-M), continuous flooding-PK fertilizer (F-PK), continuous flooding-NPK fertilizer (F-NPK), continuous flooding-NPK+organic fertilizer (F-NPK+M), and continuous flooding-organic fertilizer (F-M). CH and NO emissions in double-rice fields in tropical region of china were monitored in situ by closed static chamber-chromatography method and crop yields as well as global warming potential (GWP) and greenhouse gas intensity (GHGI) were determined. The results show that:① The cumulative CH emissions from early rice and late rice are 10.3-78.9 kg·hmand 84.6-185.5 kg·hm, respectively. Compared with F-PK and F-NPK treatments, F-NPK+M and F-M treatments significantly increased the cumulative emissions of CH from early rice season. Under the same fertilizer conditions, the cumulative CH emissions under continuous flooding condition were significantly higher than that under conventional irrigation condition. Irrigation and fertilization had extremely significant effects on CH emission in the early rice season. ② The cumulative NO emissions across all treatments were 0.18-0.76 kg·hm in early rice season and 0.15-0.58 kg·hmin late rice season, respectively. During early rice season, compared with F-PK, F-NPK significantly increased the cumulative NO emission; however, compared with D-PK, D-NPK, D-NPK+M, and D-M treatments significantly increased the cumulative NO emissions. Compared with F-PK, other three treatments under continuous flooding condition significantly increased NO cumulative emission in late rice season; compared with D-PK, D-NPK, and D-M treatment significantly increased the cumulative NO emission. Irrigation and fertilization had significant impacts on NO emissions in late rice season, and fertilization had significant impacts on NO emission in early rice season. ③ Early and late rice yields were 7310.7-9402.4 kg·hm and 3902.8-7354.6 kg·hm, respectively. Early rice yields in both F-NPK and F-M treatments were significantly higher than those in F-PK, D-PK, and D-NPK treatments. Compared with PK, the other three fertilization treatments under the same irrigation condition significantly increased late rice yield. The GWP and GHGI in early rice season were 580.8-2818.5 kg·hmand 0.08-0.30 kg·kg, respectively. There was no significant difference in GWP among four fertilizer treatments under conventional irrigation condition in the early rice season. However, F-NPK+M and F-M treatments had a significant increase in GWP compared with F-PK. The GHGI in F-NPK+M and F-M treatments were significantly higher than that in other treatments. The GWP and GHGI in late rice season were 3091.6-6334.2 kg·hm and 0.50-1.23 kg·kg, respectively. Irrigation significantly affected GWP and GHGI in both early and late rice seasons but fertilization had no significant impact on GWP and GHGI in late rice season. ④ Correlation analysis results showed that soil NH-N content and soil temperature below 5 cm soil layer had an extremely significant negative correlation with CH emissions. Soil pH was extremely significant positive correlated with CH emissions but significantly negatively correlated with NO emission. Soil NH-N and NO-N concentrations were extremely significantly negatively correlated with NO emission. Given crop yield, GWP, GHGI, and D-NPK+M can be recommended for local water and fertilizer management to reduce greenhouse gas emissions while maintaining rice yields.
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http://dx.doi.org/10.13227/j.hjkx.202011181DOI Listing
July 2021

Molecular Cloning and Functional Characterization of Three 5-HT Receptor Genes (, , and ) in Chickens.

Genes (Basel) 2021 Jun 9;12(6). Epub 2021 Jun 9.

Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China.

The serotonin (5-hydroxytryptamine, 5-HT) signaling system is involved in a variety of physiological functions, including the control of cognition, reward, learning, memory, and vasoconstriction in vertebrates. Contrary to the extensive studies in the mammalian system, little is known about the molecular characteristics of the avian serotonin signaling network. In this study, we cloned and characterized the full-length cDNA of three serotonin receptor genes (, and ) in chicken pituitaries. Synteny analyses indicated that , and were highly conserved across vertebrates. Cell-based luciferase reporter assays showed that the three chicken HTRs were functional, capable of binding their natural ligands (5-HT) or selective agonists (CP94253, BRL54443, and LY344864) and inhibiting intracellular cAMP production in a dose-dependent manner. Moreover, activation of these receptors could stimulate the MAPK/ERK signaling cascade. Quantitative real-time PCR analyses revealed that , and were primarily expressed in various brain regions and the pituitary. In cultured chicken pituitary cells, we found that LY344864 could significantly inhibit the secretion of PRL stimulated by vasoactive intestinal peptide (VIP) or forskolin, revealing that HTR1F might be involved in the release of prolactin in chicken. Our findings provide insights into the molecular mechanism and facilitate a better understanding of the serotonergic modulation via HTR1B, HTR1E and HTR1F in avian species.
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http://dx.doi.org/10.3390/genes12060891DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230051PMC
June 2021

Natural product therapies in chronic kidney diseases: An update.

Nephrol Ther 2021 Jun 26. Epub 2021 Jun 26.

Department of nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. Electronic address:

Chronic kidney disease is one of the major worldwide public health problems. Traditional Chinese medications have been widely used for chronic kidney disease treatment. As the development of modern phytochemistry technology, natural products have been isolated from traditional Chinese medications, which provide a more precise method for the investigation of traditional Chinese medications. In this article, we selected eight natural products from traditional Chinese medications for chronic kidney disease therapy to summarize the recent advances for the development of new medications.
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http://dx.doi.org/10.1016/j.nephro.2021.05.003DOI Listing
June 2021

Limited recurrence distance of glioblastoma under modern radiotherapy era.

BMC Cancer 2021 Jun 22;21(1):720. Epub 2021 Jun 22.

NHC Key Laboratory of Personalized Diagnosis and Treatment of Nasopharyngeal Carcinoma (Jiangxi Cancer Hospital of Nanchang University), 519 East Beijing Road, Nanchang, 330029, China.

Background: The optimal treatment volume for Glioblastoma multiforme (GBM) is still a subject of debate worldwide. The current study was aimed to determine the distances between recurring tumors and the edge of primary lesions, and thereby provide evidence for accurate target area delineation.

Methods: Between October 2007 and March 2019, 68 recurrent patients with GBM were included in our study. We measured the distance from the initial tumor to the recurrent lesion of GBM patients by expanding the initial gross tumor volume (GTV) to overlap the center of recurrent lesion, with the help of the Pinnacle Treatment Planning System.

Results: Recurrences were local in 47(69.1%) patients, distant in 12(17.7%) patients, and both in 9(13.2%) patients. Factors significantly influencing local recurrence were age (P = 0.049), sex (P = 0.049), and the size of peritumoral edema (P = 0.00). A total number of 91 recurrent tumors were analyzed. All local recurrences occurred within 2 cm and 94.8% (55/58) occurred within 1 cm of the original GTV based on T1 enhanced imaging. All local recurrences occurred within 1.5 cm and 98.3%(57/58) occurred within 0.5 cm of the original GTV based on T2-FLAIR imaging. 90.9% (30/33) and 81.8% (27/33) distant recurrences occurred >3 cm of T1 enhanced and T2-Flair primary tumor margins, respectively.

Conclusions: The 1 cm margin from T1 enhanced lesions and 0.5 cm margin from T2-Flair abnormal lesions could cover 94.8 and 98.3% local recurrences respectively, which deserves further prospective study as a limited but effective target area.
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http://dx.doi.org/10.1186/s12885-021-08467-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218451PMC
June 2021

Risk factors for the mortality of hemodialysis patients with COVID-19: A multicenter study from the overall hemodialysis population in Wuhan.

Semin Dial 2021 Jun 16. Epub 2021 Jun 16.

Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Introduction: Maintenance hemodialysis (MHD) patients are highly threatened in the novel coronavirus disease 2019 (COVID-19) pandemic, but evidence of risk factors for mortality in this population is still lacking.

Methods: We followed outcomes of the overall MHD population of Wuhan, including 7154 MHD patients from 65 hemodialysis centers, from January 1 to May 4, 2020. Among them, 130 were diagnosed with COVID-19. The demographic and clinical data of them were collected and compared between survivors and nonsurvivors.

Results: Compared to the corresponding period of last year, the all-cause mortality rate of the Wuhan MHD population significantly rose in February, and dropped down in March 2020. Of the 130 COVID-19 cases, 51 (39.2%) were deceased. Advanced age, decreased oxygen saturation, low diastolic blood pressure (DBP) on admission, and complications including acute cardiac injury (HR 5.03 [95% CI 2.21-11.14], p < 0.001), cerebrovascular event (HR 2.80 [95% CI 1.14-6.86], p = 0.025) and acute respiratory distress syndrome (HR 3.50 [95% CI 1.63-7.51], p = 0.001) were identified as independent risk factors for the death of COVID-19. The median virus shedding period of survivors was 25 days, longer than the general population.

Conclusions: Maintenance hemodialysis patients are a highly vulnerable population at increased risk of mortality and prolonged virus shedding period in the ongoing COVID-19 pandemic. Advanced age, decreased oxygen saturation, low DBP on admission, and complications like acute cardiac injury are parameters independently associated with poor prognosis.
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http://dx.doi.org/10.1111/sdi.12995DOI Listing
June 2021

Photophysical properties of -methyl and -acetyl substituted alloxazines: a theoretical investigation.

Phys Chem Chem Phys 2021 Jun;23(24):13734-13744

Institut für Physikalische und Theoretische Chemie, Universität Regensburg, Universitätsstr. 31, Regensburg, 93053, Germany.

The electronic structure and photophysical properties of a series of N-methyl and N-acetyl substituted alloxazines (AZs) were investigated with extensive density functional theory (DFT) and time-dependent density functional theory (TD-DFT) based calculations. We showed that non-radiative decays from the lowest singlet and triplet excited states of these AZs are dominant over their radiative counterparts. The fast non-radiative decays of the excited AZs can be attributed to the energy consumption (Ereorg) through structural reorganization facilitated by the intrinsic normal modes of the alloxazine framework, as well as their coupling with those of the functional groups. Substitution with functional groups may lead to further perturbation of the electronic structure of the AZ chromophore, which may enhance intersystem crossing with the ππ* states of the AZs. Due to the different bonding of N1 and N3 within the alloxazine framework, substitution may result in AZs with different photophysical properties. Specifically, functionalization at N1 may help in maintaining or even reducing Ereorg and would promote the absorption and radiative decay from the excited AZs. However, the strong coupling of the vibrational modes of acetyl at N3 with the intrinsic normal modes of the alloxazine framework would contribute significantly to Ereorg, and benefit the non-radiative decay of the excited AZs. We expect that the findings would pave the way for rational design of novel AZs with extraordinary photophysical properties.
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http://dx.doi.org/10.1039/d1cp01201kDOI Listing
June 2021

Association between maternal exposure to gaseous pollutants and atrial septal defect in China: A nationwide population-based study.

Environ Res 2021 Jun 9;200:111472. Epub 2021 Jun 9.

The Joint Laboratory for Pulmonary Development and Related Diseases, West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, 610041, China; National Center for Birth Defects Monitoring, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, 610041, China; Med-X Center for Informatics, Sichuan University, Chengdu, Sichuan, 610041, China. Electronic address:

Background: The association between maternal exposure to gaseous air pollutants and congenital heart defects (CHD) remains unclear. The concentration-response relationship and the time windows of susceptibility to gaseous pollutants may vary by pollutant species and CHD subtypes.

Objective: We aimed to examine the relationship between maternal exposures to four species of gaseous pollutants (NO, O, SO, and CO) and atrial septal defect (ASD), which is a common subtype of CHD, and to determine the critical time windows of susceptibility for each gaseous pollutant.

Methods: Among 1,253,633 infants born between October 1, 2013 and December 31, 2016 in China, 1937 newborns were diagnosed with isolated ASD, a prevalence of 1.55‰. Maternal exposures to the gaseous pollutants were estimated by matching the geocoded maternal addresses with the gridded ambient concentrations. The adjusted odds ratios (aOR) between exposures and ASD were quantified by using mixed-effects logistic regression models.

Results: We found significantly positive associations between ASD and maternal exposures to NO, O, SO, and CO during entire pregnancy, first-, second-, and third-trimester. However, no statistically significant association was found between maternal exposure to PM, PM and ASD risk (P > 0.05). In the fully adjusted model with respect to average exposure over entire pregnancy, the adjusted odds ratios (aOR) for each 10 μg/m increment of NO, O, SO were 1.33 (95% CI: 1.22-1.45), 1.13 (95% CI: 1.10-1.16), 1.28 (95% CI: 1.20-1.35), respectively; the aOR for each 100 μg/m increment of CO was 1.10 (95% CI: 1.06-1.15). The observed concentration-response relationships varied by exposure periods and pollutants, with the strongest association for NO during the 1st-8th embryology weeks, for O during the third trimester, for SO during the second trimester, and for CO without obvious variation.

Conclusions: The findings suggest an increased risk of ASD in association with maternal exposures to four common gaseous pollutants. From the perspective of birth defects prevention and ASD risk mitigation, it is critical to reduce maternal exposure to gaseous pollutants especially during the most susceptible time windows.
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http://dx.doi.org/10.1016/j.envres.2021.111472DOI Listing
June 2021

Cucurbit[7]uril as a Broad-Spectrum Antiviral Agent against Diverse RNA Viruses.

Virol Sin 2021 May 26. Epub 2021 May 26.

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, China.

The emergence and re-emergence of RNA virus outbreaks highlight the urgent need for the development of broad-spectrum antivirals. Polyamines are positively-charged small molecules required for the infectivity of a wide range of RNA viruses, therefore may become good antiviral targets. Cucurbit[7]uril (CB[7]), a synthetic macrocyclic molecule, which can bind with amine-based organic compounds with high affinity, has been shown to regulate bioactive molecules through competitive binding. In this study, we tested the antiviral activity of CB[7] against diverse RNA viruses, including a panel of enteroviruses (i.e. human enterovirus A71, coxsackievirus A16, coxsackievirus B3, and echovirus 11), some flaviviruses (i.e. dengue virus and Zika virus), and an alphavirus representative Semliki forest virus. CB[7] can inhibit virus replications in a variety of cell lines, and its mechanism of action is through the competitive binding with polyamines. Our findings not only for the first time provide evidence that CB[7] can be a promising broad-spectrum antiviral agent, but more importantly, offer a novel therapeutic strategy to fight against RNA viruses by supramolecular sequestration of polyamines.
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http://dx.doi.org/10.1007/s12250-021-00404-9DOI Listing
May 2021

The intervention of intestinal Wnt/β-catenin pathway alters inflammation and disease severity of CIA.

Immunol Res 2021 May 26. Epub 2021 May 26.

Department of Rheumatology and Immunology, Changhai Hospital, The Second Military Medical University, Shanghai, China.

Autoreactive T cell is one of the leading causes of immunological tolerance defects in the chronic inflammatory lesions of rheumatoid arthritis (RA). There have been several extracellular signals and intracellular pathways reported in regulating this process but largely remain unknown yet. In this study, we explored the roles of intestinal Wnt/β-catenin on disease severity during collagen-induced arthritis model (CIA), an animal model of RA. We first testified the activity pattern Wnt/β-catenin shifted by intragastric administration of LiCl and DKK-1 in the intestine by real-time PCR and WB analysis. The arthritis scores showing the disease severity in the DKK-1 group was significantly ameliorated compared with the control group at the late stage of the disease, while in the LiCl group, the scores were significantly elevated which was consistent with pathology score analysis of H&E staining. Next, ELISA was performed and showed that TNF-α and IL-17 in the LiCl group were significantly higher than that of the control group. IL-10 in the DKK-1 group was significantly higher than that in the LiCl-1 group and control group, P < 0.05. Flow cytometry of spleen T cells differentiation ratio showed that: Th1 from the DKK-1 and LiCl groups and Th17 from the LiCl group was significantly different from that of the blank model group, P < 0.05. Finally, we explored the effects of intestinal Wnt/β-catenin on T cell differentiation regulator ROR-γt and TCF1 and found that both transcription factors were up-regulated in the LiCl group. Together, these data suggested the pro-information role of Wnt/β-catenin pathway from the intestine in the CIA mouse, implying its use as a potential therapeutic target for the treatment of inflammatory diseases such as RA.
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http://dx.doi.org/10.1007/s12026-021-09190-8DOI Listing
May 2021

Effect of miR-630 expression on esophageal cancer cell invasion and migration.

J Clin Lab Anal 2021 Jun 21;35(6):e23815. Epub 2021 May 21.

The First Hospital Affiliated to AMU, Chongqing, China.

Background: Esophageal cancer (EC) is a common malignancy of the digestive tract, with high incidence. The objective of this study was to investigate the effect of miR-630 expression on esophageal cancer (EC) cell invasion and migration.

Methods: The study group comprised 58 EC patients admitted to our hospital from April 2014 to 2016, and the control group comprised 60 healthy people visiting the hospital during the same period. miR-630 levels in the peripheral blood of the two groups were compared, and the diagnostic value of miR-630 for EC was analyzed. EC cell lines were used to evaluate the influence of miR-630 expression on EC cell invasion and migration.

Results: miR-630 expression was low in EC (p < 0.050). A receiver operating characteristic curve analysis showed that miR-630 expression had a good diagnostic value for EC (p < 0.050) and was associated with disease course, pathological stage, differentiation degree, tumor metastasis, and patient prognosis and survival (p < 0.05). The ROC curve analysis showed that when cutoff value was 5.38, the diagnostic sensitivity and specificity of miR-630 for EC were 73.33% and 76.67%, respectively; area under the ROC curve was 0.778 (95%CI 0.695-0.861). Transfection of miR-630 into EC cells indicated that miR-630 overexpression can reduce EC cell invasion and migration (p < 0.05). miR-630 expression is low in EC and has good diagnostic value for EC.

Conclusion: miR-630 overexpression can reduce EC cell invasion and migration, showing a possible key role of miR-630 in EC diagnosis and treatment in the future.
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http://dx.doi.org/10.1002/jcla.23815DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183945PMC
June 2021

A Comprehensive Review of Computational Methods for Drug-drug Interaction Detection.

IEEE/ACM Trans Comput Biol Bioinform 2021 May 18;PP. Epub 2021 May 18.

The detection of drug-drug interactions (DDIs) is a crucial task for drug safety surveillance, which provides effective and safe co-prescriptions of multiple drugs. Since laboratory researches are often complicated, costly and time-consuming, it's urgent to develop computational approaches to detect drug-drug interactions. In this paper, we conduct a comprehensive review of state-of-the-art computational methods falling into three categories: literature-based extraction methods, machine learning-based prediction methods and pharmacovigilance-based data mining methods. Literature-based extraction methods detect DDIs from published literature using natural language processing techniques; machine learning-based prediction methods build prediction models based on the known DDIs in databases and predict novel ones; pharmacovigilance-based data mining methods usually apply statistical techniques on various electronic data to detect drug-drug interaction signals. We first present the taxonomy of drug-drug interaction detection methods and provide the outlines of three categories of methods. Afterwards, we respectively introduce research backgrounds and data sources of three categories, and illustrate their representative approaches as well as evaluation metrics. Finally, we discuss the current challenges of existing methods and highlight potential opportunities for future directions.
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http://dx.doi.org/10.1109/TCBB.2021.3081268DOI Listing
May 2021

Economy or health: Environmental challenges in rapid developing China and beyond.

Environ Res 2021 May 13;200:111308. Epub 2021 May 13.

Department of Environment, College of Architecture and Environment, Sichuan University, Chengdu, Sichuan, 610065, China; Environmental Health Trust, China. Electronic address:

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http://dx.doi.org/10.1016/j.envres.2021.111308DOI Listing
May 2021

UV Light Activated Multi-Cycle Photoelectric Properties of TiO₂ and CdS/TiO ₂ Films in Formaldehyde.

J Nanosci Nanotechnol 2021 11;21(11):5642-5647

Key Laboratory of Hunan Province on Information Photonics and Freespace Optical Communications, School of Physics and Electronic Science, Hunan Institute of Science and Technology, Yueyang 414006, China.

In this work, UV light activated multi-cycle photoelectric properties of TiO₂ and CdS/TiO₂ films in formaldehyde were researched. TiO₂ film was prepared by screen printing, CdS/TiO₂ compounded film was synthesized by SILAR method. XRD and FE-SEM was used to characterize the TiO₂ and CdS/TiO₂ samples. Multi-cycle photoelectric properties of TiO₂ and CdS/TiO₂ with uv light on and off were evaluated by testing the photocurrent. On one hand, under the same bias voltage, CdS/TiO ₂showed a higher photocurrent than that by TiO₂. The reason for this result should be ascribed to the compounded structure in CdS/TiO₂, with which the separation and transfer of photogenerated electron-hole pairs could be improved. On the other hand, with the testing cycle number increased, the photocurrent amplitudes of TiO₂ and CdS/TiO₂ increased. These results suggested that the time to reach a stable photocurrent value for TiO₂ and CdS/TiO₂ is much longer than one cycle time (300 S). To illustrate the increased photocurrent amplitude value cycle by cycle, the photocurrent of CdS/TiO₂ to a much longer time (more than 4000 seconds) was also tested. To explain these results, corresponding possible illustrations were presented.
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http://dx.doi.org/10.1166/jnn.2021.19465DOI Listing
November 2021

Study on the Rehydration Quality Improvement of Mushroom by Combined Drying Methods.

Foods 2021 Apr 3;10(4). Epub 2021 Apr 3.

Food Biobased Research, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.

The aim of study is to improve the rehydration quality of dried mushrooms for their instant food manufacturers and fast restaurants. Serial combined drying methods were investigated to achieve this objective: either instant controlled pressure drop drying (DIC) or freeze drying (FD) was used as the treatments for microstructure improvement, and they were applied either before or after an additional drying step at low (35 °C) or high (65 °C) temperatures. Dried mushrooms were assessed for quality indicators like relative volume, rehydration rate, dry matter loss and sensory scores. Microstructure properties were inferred to understand the physical mechanisms of quality changes. Principal component analysis (PCA) was used to cluster treatments and to identify combinations of drying techniques, rendering improved quality. Consequently, it was shown that DIC treatment before hot air drying at 35 °C was shown to be the most promising combined drying method to enhance the rehydration quality, leading to a high volume recovery ratio, low dry matter loss after rehydration, and high rehydration rates. This good performance could be explained by the retention of pore interconnectivity resulting from the slight expansion of porous structure during DIC and the retention of cell membrane integrity.
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http://dx.doi.org/10.3390/foods10040769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065859PMC
April 2021

Guaico Culex virus NSP2 has RNA helicase and chaperoning activities.

J Gen Virol 2021 04;102(4)

State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences (CAS), Wuhan 430071, PR China.

RNA-remodelling proteins, including RNA helicases and chaperones, function to remodel structured RNAs and/or RNA-protein interactions and play indispensable roles in viral life cycles. Guaico Culex virus (GCXV) is the first uncovered animal-infected multicomponent virus with segmented positive-sense genomic RNAs. GCXV belongs to the Jingmenvirus group, a diverse clade of segmented viruses that are related to the prototypically unsegmented . However, little is known about the exact functions of the GCXV-encoded proteins. Here, we show that the putative non-structural protein (NSP) 2 on segment 2 of GCXV functions as an RNA helicase that unwinds RNA helix bidirectionally in an adenosine triphosphate (ATP)-dependent manner, and an RNA chaperone that remodels structured RNAs and facilitates RNA strand annealing independently of ATP. Together, our findings are the first demonstration of RNA-remodelling activity encoded by Jingmenvirus and highlight the functional significance of NSP2 in the GCXV life cycle.
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http://dx.doi.org/10.1099/jgv.0.001589DOI Listing
April 2021

Semen quality and windows of susceptibility: A case study during COVID-19 outbreak in China.

Environ Res 2021 06 2;197:111085. Epub 2021 Apr 2.

Department of Environmental Sciences and Engineering, College of Architecture and Environment, Sichuan University, PR China. Electronic address:

Background: To evaluate the impact of air pollution exposure on semen quality parameters during COVID-19 outbreak in China, and to identify potential windows of susceptibility for semen quality.

Methods: A retrospective observational study was carried out on 1991 semen samples collected between November 23, 2019 and July 23, 2020 (a period covering COVID-19 lock-down in China) from 781 sperm donor candidates at University-affiliated Sichuan Provincial Human Sperm Bank. Multivariate mixed-effects regression models were constructed to investigate the relationship between pollution exposure, windows of susceptibility, and semen quality, while controlling for biographic and meteorologic confounders.

Result(s): The results indicated multiple windows of susceptibility for semen quality, especially sperm motility, due to ambient pollution exposure. Exposure to particulate matters (PM and PM), O and NO during late stages of spermatogenesis appeared to have weak but positive association with semen quality. Exposure to CO late in sperm development appeared to have inverse relationship with sperm movement parameters. Exposure to SO appeared to influence semen quality throughout spermatogenesis.

Conclusion(s): Potential windows of susceptibility for semen quality varied depending on air pollutants. Sperm motility was sensitive to pollution exposure. Findings from current study further elucidate the importance of sensitive periods during spermatogenesis and provide new evidence for the determinants of male fertility.
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http://dx.doi.org/10.1016/j.envres.2021.111085DOI Listing
June 2021

Antiviral Peptides Targeting the Helicase Activity of Enterovirus Nonstructural Protein 2C.

J Virol 2021 05 24;95(12). Epub 2021 May 24.

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China

Enteroviruses belong to the genus of the family and include four human enterovirus groups (EV-A to -D): the epidemic of enteroviruses such as human enterovirus A71 (EV-A71) and coxsackievirus A16 (CVA16) is a threat to global public health. Enteroviral protein 2C is the most conserved nonstructural protein among all enteroviruses and possesses RNA helicase activity that plays pivotal roles during enteroviral life cycles, which makes 2C an attractive target for developing antienterovirus drugs. In this study, we designed a peptide, named 2CL, based on the structure of EV-A71 2C. This peptide effectively impaired the oligomerization of EV-A71 2C protein and inhibited the RNA helicase activities of 2C proteins encoded by EV-A71 and CVA16, both of which belong to EV-A, and showed potent antiviral efficacy against EV-A71 and CVA16 in cells. Moreover, the 2CL treatment elicited a strong protective efficacy against lethal EV-A71 challenge. In addition, the antiviral strategy of targeting the 2C helicase activity can be applied to inhibit the replication of EV-B. Either 2CL or B-2CL, the peptide redesigned based on the 2CL-corresponding sequence of EV-Bs, could exert effective antiviral activity against two important EV-Bs, coxsackievirus B3 and echovirus 11. Together, our findings demonstrated that targeting the helicase activity of 2C with a rationally designed peptide is an efficient antiviral strategy against enteroviruses, and 2CL and B-2CL show promising clinical potential to be further developed as broad-spectrum antienterovirus drugs. Enteroviruses are a large group of positive-sense single-stranded RNA viruses and include numerous human pathogens, such as enterovirus A71 (EV-A71), coxsackieviruses, and echoviruses. However, no approved EV antiviral drugs are available. Enteroviral 2C is the most conserved nonstructural protein among all enteroviruses and contains the RNA helicase activity critical for the viral life cycle. Herein, according to the structure of EV-A71 2C, we designed a peptide that effectively inhibited the RNA helicase activities of EV-A71- and coxsackievirus A16 (CVA16)-encoded 2C proteins. Moreover, this peptide exerted potent antiviral effects against EV-A71 and CVA16 in cells and elicited therapeutic efficacy against lethal EV-A71 challenge Furthermore, we demonstrate that the strategy of targeting the 2C helicase activity can be used for other relevant enteroviruses, including coxsackievirus B3 and echovirus 11. In summary, our findings provide compelling evidence that the designed peptides targeting the helicase activity of 2C could be broad-spectrum antivirals for enteroviruses.
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http://dx.doi.org/10.1128/JVI.02324-20DOI Listing
May 2021

Interleukin-18 promotes the antitumor ability of natural killer cells in colorectal cancer via the miR-574-3p/TGF-β1 axis.

Bioengineered 2021 12;12(1):763-778

Department of Gastroenterology, Shenzhen People's Hospital Longhua Branch (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen 518020, Guangdong, China.

Interleukin (IL)-18 has a clear antitumor effect; however, its mechanisms of action are not understood in patients with colorectal cancer (CRC). Here, we investigated the potential mechanism of IL-18 in CRC. The results showed that IL-18 treatment alone had no effect on HCT116 cells apoptosis, whereas IL-18 in the presence of natural killer (NK) cells resulted in apoptosis and inhibition of cells proliferation in vitro. Profiling of miRNA expression following coculture with NK cells and treatment with IL-18 resulted in significant downregulation of miR-574-3p expression and upregulated expression of the target gene transforming growth factor beta 1 (TGF-β1). miR-574-3p binds to TGF-β1, and miR-574-3p overexpression increased the proliferation and decreased the apoptotic rate of HCT116 cells in NK cells coculture with IL-18 treatment; overexpression of TGF-β1 restored the effect of miR-574-3p overexpression. The miRNA profile of HCT116 undergoes significant alteration before and after coculturing with NK cells and treatment with IL-18. IL-18 alone did not affect HCT116 cells apoptosis but did promote the antitumor ability of NK cells in coculture with HCT116 cells via the miR-574-3p/TGF-β1 axis. Our study suggested that IL-18 can be a new potential target for cancer immunotherapy for CRC.
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http://dx.doi.org/10.1080/21655979.2021.1880717DOI Listing
December 2021

Acupuncture for chronic constipation in patients with diabetes mellitus: A protocol for systematic review.

Medicine (Baltimore) 2021 Mar;100(9):e24886

The Affiliated Hospital of North Sichuan Medical College, Sichuan, China.

Background: Assessing the effectiveness and safety of acupuncture for chronic constipation in patients with diabetes mellitus is the main purpose of this systematic review protocol.

Methods: The following electronic databases will be searched from their respective inception dates to December 1st 2020: PubMed, the Cochrane Library, Embase, World Science Net, the Allied and Complementary Medicine Database, the Web of Science, China National Knowledge Infrastructure, the Chongqing VIP Chinese Science and Technology Periodical Database and the Wanfang Database. All published randomized controlled trials in English or Chinese related to acupuncture for constipation in patient with diabetes mellitus will be included. The Bristol stool scale, spontaneous complete bowel movements, and observing symptoms (yes/no) including defecation feeling, defecation weakness, feeling of incomplete evacuation, bloating, and flatulence were considered as primary measures. The treatment efficiency consideration according to Bristol stool scale was considered as secondary measure. Two reviewers will conduct the study selection, data extraction and assessment independently. The assessment of risk of bias and data synthesis will be conducted with Review Manager Software (RevMan) V.5.2.

Results: The results will provide a high-quality synthesis of current evidence for researchers in this subject area.

Conclusion: The conclusion of our study will provide an evidence to judge whether.Acupuncture is an effective intervention for chronic constipation in patients with diabetes mellitus.

Ethics And Dissemination: Formal ethical approval is not necessary as the data cannot be individualized. The results of this protocol will be disseminated in a peer-reviewed journal or presented at relevant conferences.

Prospero Registration Number: INPLASY202110079.
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http://dx.doi.org/10.1097/MD.0000000000024886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939182PMC
March 2021

Inhomogeneous Kondo-lattice in geometrically frustrated PrIrO.

Nat Commun 2021 Mar 2;12(1):1377. Epub 2021 Mar 2.

Department of Physics, Applied Physics and Astronomy, Binghamton University, Binghamton, NY, USA.

Magnetic fluctuations induced by geometric frustration of local Ir-spins disturb the formation of long-range magnetic order in the family of pyrochlore iridates. As a consequence, PrIrO lies at a tuning-free antiferromagnetic-to-paramagnetic quantum critical point and exhibits an array of complex phenomena including the Kondo effect, biquadratic band structure, and metallic spin liquid. Using spectroscopic imaging with the scanning tunneling microscope, complemented with machine learning, density functional theory and theoretical modeling, we probe the local electronic states in PrIrO and find an electronic phase separation. Nanoscale regions with a well-defined Kondo resonance are interweaved with a non-magnetic metallic phase with Kondo-destruction. These spatial nanoscale patterns display a fractal geometry with power-law behavior extended over two decades, consistent with being in proximity to a critical point. Our discovery reveals a nanoscale tuning route, viz. using a spatial variation of the electronic potential as a means of adjusting the balance between Kondo entanglement and geometric frustration.
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http://dx.doi.org/10.1038/s41467-021-21698-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925525PMC
March 2021

An understanding of the changes in water holding capacity of rehydrated shiitake mushroom (Lentinula edodes) from cell wall, cell membrane and protein.

Food Chem 2021 Jul 5;351:129230. Epub 2021 Feb 5.

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS) / Key, Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.

Water holding capacity (WHC) is an important factor for the evaluation of rehydrated shiitake mushroom as WHC largely affects sensory attributes. Here, WHC of the rehydrated shiitake mushroom as affected by different hot-air drying temperature was investigated from aspects of cell membrane integrity, status of cell wall fibrous material and protein denaturation. Among the three factors, protein denaturation played the most important role to affect the WHC of rehydrated mushroom as protein denaturation was closely related to the maintenance of porous structure after rehydration. Effect of status of the cell wall fibrous material to the WHC changes was relatively weak and was largely correlated to the WHC changes at high temperature (>80 °C). Cell membrane was unlikely to contribute to WHC changes of the rehydrated shiitake mushroom due to the loss of integrity.
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http://dx.doi.org/10.1016/j.foodchem.2021.129230DOI Listing
July 2021

Comparison of different groundwater vulnerability evaluation models of typical karst areas in north China: a case of Hebi City.

Environ Sci Pollut Res Int 2021 Jun 16;28(24):30821-30840. Epub 2021 Feb 16.

School of Environmental Studies, China University of Geosciences, Wuhan, 430074, China.

Groundwater pollution is a serious problem in north China. However, the study on the vulnerability of karst groundwater is mainly in south China, and there are few studies in north China. To study the applicability of different models of karst areas in north China, this paper chose a special study area-Hebi City, where the exposed karst area is widely developed in the hilly area, but the covered karst area is in the eastern part of the study area. The DRASTIC model, the AHP-DRASTIC model, and the improved COPK model were adopted to evaluate the vulnerability of shallow karst groundwater in Hebi City. Cl, SO, NO, and TDS were selected to verify the rationality of the evaluation results. It shows that the improved COPK model is more suitable for the shallow karst groundwater vulnerability evaluation in the karst areas in northern China represented by the study area than the other two. The study area was divided into 4 classes by the improved COPK model: highest (14.07%), high (53.05%), low (21.37%), and lowest (11.51%). Then, the analytic hierarchy process and comprehensive index model were used to evaluate the groundwater pollution load intensity, and the study area was divided into 3 classes: high (23.33%), moderate (64.66%), and low (12.01%). According to the analysis of the relationship between groundwater pollution load intensity and groundwater quality, it can be found that human activities have an obvious influence on groundwater quality in the study area. Finally, combined with human activities, the study area was divided into 3 remediation areas, 1 control area, and 1 protected area. This paper can provide a scientific basis for rational exploitation and utilization of groundwater resources. It can also provide a reference for future generations to evaluate the groundwater vulnerability in the northern China karst areas.
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http://dx.doi.org/10.1007/s11356-021-12719-xDOI Listing
June 2021

SARS-CoV-2 ORF9b inhibits RIG-I-MAVS antiviral signaling by interrupting K63-linked ubiquitination of NEMO.

Cell Rep 2021 02 3;34(7):108761. Epub 2021 Feb 3.

Institute of Engineering Biology and Health, Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China. Electronic address:

Coronavirus disease 2019 (COVID-19) is a current global health threat caused by the novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Emerging evidence indicates that SARS-CoV-2 elicits a dysregulated immune response and a delayed interferon (IFN) expression in patients, which contribute largely to the viral pathogenesis and development of COVID-19. However, underlying mechanisms remain to be elucidated. Here, we report the activation and repression of the innate immune response by SARS-CoV-2. We show that SARS-CoV-2 RNA activates the RIG-I-MAVS-dependent IFN signaling pathway. We further uncover that ORF9b immediately accumulates and antagonizes the antiviral type I IFN response during SARS-CoV-2 infection on primary human pulmonary alveolar epithelial cells. ORF9b targets the nuclear factor κB (NF-κB) essential modulator NEMO and interrupts its K63-linked polyubiquitination upon viral stimulation, thereby inhibiting the canonical IκB kinase alpha (IKKα)/β/γ-NF-κB signaling and subsequent IFN production. Our findings thus unveil the innate immunosuppression by ORF9b and provide insights into the host-virus interplay during the early stage of SARS-CoV-2 infection.
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http://dx.doi.org/10.1016/j.celrep.2021.108761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7857071PMC
February 2021

Quantifying the effects of antibiotic treatment on the extracellular polymer network of antimicrobial resistant and sensitive biofilms using multiple particle tracking.

NPJ Biofilms Microbiomes 2021 02 5;7(1):13. Epub 2021 Feb 5.

Advanced Therapies Group, Cardiff University School of Dentistry, Cardiff, UK.

Novel therapeutics designed to target the polymeric matrix of biofilms requires innovative techniques to accurately assess their efficacy. Here, multiple particle tracking (MPT) was developed to characterize the physical and mechanical properties of antimicrobial resistant (AMR) bacterial biofilms and to quantify the effects of antibiotic treatment. Studies employed nanoparticles (NPs) of varying charge and size (40-500 nm) in Pseudomonas aeruginosa PAO1 and methicillin-resistant Staphylococcus aureus (MRSA) biofilms and also in polymyxin B (PMB) treated Escherichia coli biofilms of PMB-sensitive (PMB) IR57 and PMB-resistant (PMB) PN47 strains. NP size-dependent and strain-related differences in the diffusion coefficient values of biofilms were evident between PAO1 and MRSA. Dose-dependent treatment effects induced by PMB in PMB E. coli biofilms included increases in diffusion and creep compliance (P < 0.05), not evident in PMB treatment of PMB E. coli biofilms. Our results highlight the ability of MPT to quantify the diffusion and mechanical effects of antibiotic therapies within the AMR biofilm matrix, offering a valuable tool for the pre-clinical screening of anti-biofilm therapies.
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http://dx.doi.org/10.1038/s41522-020-00172-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864955PMC
February 2021

The Capsid Protein of Rubella Virus Antagonizes RNA Interference in Mammalian Cells.

Viruses 2021 01 21;13(2). Epub 2021 Jan 21.

State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences (CAS), Wuhan 430071, Hubei, China.

Rubella virus (RuV) is the infectious agent of a series of birth defect diseases termed congenital rubella syndrome, which is a major public health concern all around the world. RNA interference (RNAi) is a crucial antiviral defense mechanism in eukaryotes, and numerous viruses have been found to encode viral suppressors of RNAi (VSRs) to evade antiviral RNAi response. However, there is little knowledge about whether and how RuV antagonizes RNAi. In this study, we identified that the RuV capsid protein is a potent VSR that can efficiently suppress shRNA- and siRNA-induced RNAi in mammalian cells. Moreover, the VSR activity of the RuV capsid is dependent on its dimerization and double-stranded RNA (dsRNA)-binding activity. In addition, ectopic expression of the RuV capsid can effectively rescue the replication defect of a VSR-deficient virus or replicon, implying that the RuV capsid can act as a VSR in the context of viral infection. Together, our findings uncover that RuV encodes a VSR to evade antiviral RNAi response, which expands our understanding of RuV-host interaction and sheds light on the potential therapeutic target against RuV.
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http://dx.doi.org/10.3390/v13020154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910915PMC
January 2021

Effective virus-neutralizing activities in antisera from the first wave of survivors of severe COVID-19.

JCI Insight 2021 02 22;6(4). Epub 2021 Feb 22.

State Key Laboratory of Virology, Institute of Medical Virology and School of Basic Medical Sciences, Wuhan University, Wuhan, China.

The coronavirus disease 19 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become the worst public health crisis in a century. However, knowledge about the dynamics of antibody responses in patients with COVID-19 is still poorly understood. In this study, we performed a serological study with serum specimens collected at the acute and the convalescent phases from 104 patients with severe COVID-19 who were part of the first wave of COVID-19 cases in Wuhan, China. Our findings revealed that neutralizing antibodies to SARS-CoV-2 are persistent for at least 6 months in patients with severe COVID-19, despite that IgG levels against the receptor binding domain (RBD) and nucleocapsid protein (N) IgG declined from the acute to the convalescent phase. Moreover, we demonstrate that the level of RBD-IgG is capable of correlating with SARS-CoV-2-neutralizing activities in COVID-19 serum. In summary, our findings identify the magnitude, functionality, and longevity of antibody responses in patients with COVID-19, which sheds light on the humoral immune response to COVID-19 and would be beneficial for developing vaccines.
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http://dx.doi.org/10.1172/jci.insight.146267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934937PMC
February 2021

Imaging Mass Cytometric Analysis of Postmortem Tissues Reveals Dysregulated Immune Cell and Cytokine Responses in Multiple Organs of COVID-19 Patients.

Front Microbiol 2020 23;11:600989. Epub 2020 Dec 23.

Department of Critical Care Medicine, Tongji Medical College, Union Hospital, Huazhong University of Science and Technology, Wuhan, China.

SARS-coronavirus-2-induced immune dysregulation and inflammatory responses are involved in the pathogenesis of coronavirus disease-2019 (COVID-19). However, very little is known about immune cell and cytokine alterations in specific organs of COVID-19 patients. Here, we evaluated immune cells and cytokines in postmortem tissues, i.e., lungs, intestine, liver, kidneys, and spleen of three patients with COVID-19. Imaging mass cytometry revealed monocyte, macrophage, and dendritic cell (DC) infiltration in the lung, intestine, kidney, and liver tissues. Moreover, in patients with COVID-19, natural killer T cells infiltrated the liver, lungs, and intestine, whereas B cells infiltrated the kidneys, lungs, and intestine. CD11b macrophages and CD11c DCs also infiltrated the lungs and intestine, a phenomenon that was accompanied by overproduction of the immunosuppressive cytokine interleukin (IL)-10. However, CD11b macrophages and CD11c DCs in the lungs or intestine of COVID-19 patients did not express human leukocyte antigen DR isotype. In contrast, tumor necrosis factor (TNF)-α expression was higher in the lungs, intestine, liver, and kidneys, but not in the spleen, of all COVID-19 patients (compared to levels in controls). Collectively, these findings suggested that IL-10 and TNF-α as immunosuppressive and pro-inflammatory agents, respectively,-might be prognostic and could serve as therapeutic targets for COVID-19.
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http://dx.doi.org/10.3389/fmicb.2020.600989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785801PMC
December 2020

Regional and age difference of human rabies prevalence of the past fourteen years in China.

Prev Vet Med 2021 Feb 28;187:105161. Epub 2020 Sep 28.

Department of Clinical Laboratory, Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi, China. Electronic address:

Objective: The aim of this study is to describe the epidemiological characteristics about regional and age difference of human rabies in the past fourteen years in China, and provide a reliable epidemiology basis for further control and prevention of human rabies.

Methods: The database of "China Public Health Science Data Center" affiliated Chinese CDC was searched with the key words of "rabies" or "epidemiology" or "morbidity" or "mortality" from 2004 to 2018 and the corresponding data about human rabies cases was collected referred to regional and age difference for describing the epidemiological characteristics of human rabies.

Results: In this study, a total of nearly 26,315 rabies cases (1754 ± 253) and 25,691 rabies-related deaths (1712 ± 255) (Mean ± SE) were reported, and a decreasing trend about the morbidity and mortality of human rabies existed from 0.2039 and 0.2039 (1/100,000) in 2004 to 0.0304 and 0.0295 in 2018. Otherwise, regional difference of human rabies prevalence significantly existed, and juvenile and middle-aged population especially in 50-60 years old were more easily attacked and infected with rabies (all p < 0.05).

Conclusion: This study proved that human rabies still is a major public health problem in China though a decreasing trend about the morbidity and mortality of human rabies existed in the past fourteen years.
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http://dx.doi.org/10.1016/j.prevetmed.2020.105161DOI Listing
February 2021
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