Publications by authors named "Yang Ni"

224 Publications

A single-center retrospective long-term analysis of 80 cases of ovarian Sertoli-Leydig cell tumors.

Chin Med J (Engl) 2021 May 13. Epub 2021 May 13.

Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China Department of Pathology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.

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http://dx.doi.org/10.1097/CM9.0000000000001489DOI Listing
May 2021

The conformational structural change of β-lactoglobulin via acrolein treatment reduced the allergenicity.

Food Chem X 2021 Jun 20;10:100120. Epub 2021 Apr 20.

College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.

β-lactoglobulin (BLG) is a major allergen of milk. Since lipid peroxidation such as acrolein commonly exists during milk processing, it is necessary to evaluate its influence on BLG structure and potential allergenicity. The structure of acrolein-treated BLG was detected using SDS-PAGE, fluorescence, ultraviolet spectrum (UV), circular dichroism (CD) and LC-MS-MS, and the potential allergenicity was assessed by and assays. Results showed that acrolein could cause structural changes by BLG aggregation, which decreased the IgE binding capacity. Further, the release of mediators and cytokines decreased with acrolein treatment in RBL-2H3 cells. Mice showed lower allergenicity by the levels of BLG-specific antibody and the release of histamine and mMCP-1. These results explained that acrolein-induced BLG aggregation could damage the allergic epitopes and decrease the allergenicity of BLG in milk. The study will provide a new aspect to explore the natural phenomenon of allergen changes during food processing.
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http://dx.doi.org/10.1016/j.fochx.2021.100120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085757PMC
June 2021

Discovery of genes involved in anthocyanin biosynthesis from the rind and pith of three sugarcane varieties using integrated metabolic profiling and RNA-seq analysis.

BMC Plant Biol 2021 May 12;21(1):214. Epub 2021 May 12.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, 100193, Beijing, P. R. China.

Background: Sugarcane (Saccharum officinarum) is one of the most valuable feedstocks for sugar production. In addition to the production of industrial raw materials such as alcohol, papermaking, the fiber of livestock feed, respectively, sugarcane can produce bioactive compounds such as anthocyanins. Elucidation of the anthocyanin biosynthesis pathway is critical for the molecular breeding of sugarcane varieties with favorable traits. We aimed to identify candidate genes involved in anthocyanin biosynthesis by transcriptomic and metabolomic analyses.

Results: Three varieties of sugarcane displaying different colors were used in this study: FN15 (greed rind), ROC22 (red rind), and Badila (purple rind). Sample materials were subjected to metabolomic analysis using UPLC-Q-TOF/MS and RNA-seq analysis. The metabolomic profiling results showed Cyanidin, Cyanidin (6'-malonylglucoside), Cyanidin O-glucoside, and Peonidin O-glucoside were the main components responsible for the rind color. Then, through RNA-seq analysis, we identified a total of 3137, 3302, 3014 differentially expressed genes (DEGs) between the rind and pith tissues for the corresponding varieties Badila rind, ROC22, and FN15. We then compared the expression levels of genes among the rind tissues from the three varieties. We identified 2901, 2821, and 3071 DEGs between Badila rind vs. ROC22 rind, Badila rind vs. FN15 rind, ROC22 rind vs. FN15 rind, respectively. We identified two enriched pathways, including phenylpropanoid biosynthesis and flavonoid biosynthesis. Sequencing similarity search identified a total of 50 unigenes belonging to 15 enzyme families as putative genes involved in anthocyanin biosynthesis in sugarcane rind. Seven of them were identified as candidate genes related to anthocyanin biosynthesis in the rind of sugarcane through co-localization analysis with the anthocyanin content in sugarcane. In total, 25 unigenes were selected and subjected to RT-qPCR analysis, and qRT-PCR results were consistent with those obtained with the RNA-Seq experiments.

Conclusions: We proposed a pathway for anthocyanin biosynthesis in sugarcane rind. This is the first report on the biosynthesis of anthocyanin in sugarcane using the combined transcriptomic and metabolomic methods. The results obtained from this study will lay the foundation for breeding purple pith sugarcane varieties with high anthocyanin contents.
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http://dx.doi.org/10.1186/s12870-021-02986-8DOI Listing
May 2021

Adaptation of a parasitic lifestyle by gronovii Willd. ex Roem. & Schult.: large scale gene deletion, conserved gene orders, and low intraspecific divergence.

Mitochondrial DNA B Resour 2021 Apr 20;6(4):1475-1482. Epub 2021 Apr 20.

Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine from Ministry of Education, Engineering Research Center of Chinese Medicine Resources from Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, P. R. China.

Willd. ex Roem. & Schult. is a parasitic plant and widely distributed in Europe and North America. Here, we compared the plastome sequences of a plant collected from China and other 16 plastomes, including one plastome from North America. The plastome was 86,727 bp in size with a pair of inverted repeats (IRs) of 14,354 bp, a large single-copy (LSC) region of 50,956 bp, and a small single-copy (SSC) region of 7,063 bp. We predicted 97 genes in the plastome, including 61 protein-coding genes, eight ribosomal RNA genes, and 28 transfer RNA genes. We detected a total of 21 microsatellite, 16 tandem, and ten interspersed repeats in the genome. Gene contents analysis of 17 plastomes showed the loss of the entire gene family. The phylogenomic analysis using 22 shared protein sequences shows that the two formed a cluster. Thirteen of plastomes showed no rearrangement. The other three showed smaller inversions and smaller numbers of gene deletion. Next, we identified the two INDELs, one SNP site between the two plastomes. And we identified two putative RNA editing sites. Lastly, to distinguish between and two medicinal species, and , we identified ten molecular markers based on the genome sequences. The overall large-scale gene loss, conserved gene order, low intraspecific divergence are consistent with the adaptation of C. to a parasitic lifestyle.
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http://dx.doi.org/10.1080/23802359.2021.1911702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079069PMC
April 2021

C/EBP β mediates the aberrant inflammatory response and cell cycle arrest in LPS-stimulated human renal tubular epithelial cells by regulating NF-κB pathway.

Arch Med Res 2021 May 1. Epub 2021 May 1.

Emergency department, The second affiliated hospital of Xi'an Jiaotong University, (Xibei Hospital), Xi'an 710004, China. Electronic address:

Background And Aims: The main cause of sepsis-induced Acute kidney injury (AKI) is acute infection after surgery and subsequent progression. However, the mechanism by which AKI is caused and developed from sepsis are not completely known. Herein, we determined the role of CCAAT/enhancer-binding protein β (C/EBP β) in sepsis-induced AKI METHODS: C/EBP β expression was up or down-regulated in LPS-stimulated human renal tubular epithelial cells in vitro by recombinant adenoviruses or siRNA. Subsequent analyses included the test of TNF-α and IL-6 levels by ELISA, cell cycle assay by flow cytometry.

Results: C/EBP β was aberrantly expressed in renal tubular epithelial HK-2 cells exposed to LPS. C/EBP β overexpression significantly enhanced, but C/EBP β silencing obviously decreased the production and secretion of inflammatory cytokines TNF-α and IL-6 induced by LPS stimulus in HK-2 cells. And the cell cycle arrest of HK-2 cells induced by LPS was also enhanced after C/EBP β overexpression while attenuated after C/EBP β silencing. Consistent pattern of changes in Cyclin D1 and p21 expression were observed in LPS-stimulated HK-2 cells after C/EBP β silencing and C/EBP β overexpression. Additionally, the increased p-NF-κB levels induced by LPS were found to be obviously decreased after C/EBP β silencing in HK-2 cells. And the enhanced TNF-α and IL-6 secretion as well as cell cycle arrest by C/EBP β overexpression were blocked by BAY11-7082 inhibitor of NF-κB pathway.

Conclusions: C/EBP β could mediate the LPS-induced aberrant inflammatory response and cell cycle arrest in tubular epithelial cells by NF-κB pathway.
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http://dx.doi.org/10.1016/j.arcmed.2021.03.008DOI Listing
May 2021

Impact of Ambient Air Quality Standards revision on the exposure-response of air pollution in Tianjin, China.

Environ Res 2021 May 1;198:111269. Epub 2021 May 1.

School of Public Health, Tianjin Medical University, Tianjin, 300070, China; Tianjin Centers for Disease Control and Prevention, Tianjin, 300011, China. Electronic address:

To improve air quality, China issued a new version of Ambient Air Quality Standards in 2012, and Tianjin officially implemented these new standards in 2013. In order to observe whether the atmospheric quality and health had been improved after the release of the new standards, this study compared the exposure-response relationships between pollutant concentrations and daily deaths (and years of life lost, YLL) in the six central districts of Tianjin in 2007-2010 and 2014-2017, which were the two periods around the release of the new standards. The exposure-response coefficients were calculated by using the generalized additive model with the time series data. The results showed that the effects of PM and NO during 2014-2017 were lower than those during 2007-2010 (PM: 0.10<0.30, 2.07<4.52; NO2: 0.24<0.87, 6.39<11.98), while the results of SO were opposite (SO: 0.61>0.31, 10.31>1.64). And the excess deaths and YLL caused by the portion of the pollutants that exceeded the new standards also declined (PM: 156<206 persons, 3493<3913 person-years; NO: 64<136 persons, 1844<2895 person-years; SO did not exceed the new standards). The results proved that the new standards had a certain protective effect on the health of the population in the central districts of Tianjin. These findings may provide evidence for the formulation of public health policies and further improvement and development of the Ambient Air Quality Standards.
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http://dx.doi.org/10.1016/j.envres.2021.111269DOI Listing
May 2021

A porphysome-based photodynamic O economizer for hypoxic tumor treatment by inhibiting mitochondrial respiration.

Chem Commun (Camb) 2021 Apr;57(34):4134-4137

Key Laboratory of Molecular Target & Clinical Pharmacology and the State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences & The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou 511436, P. R. China.

A porphysome-based photodynamic O2 economizer (P-PAT) is prepared for hypoxic tumor therapy. The self-assembled porphyrin bilayers of P-PAT possess high loading capacity to atovaquone (ATO) (nearly 70%), which could restrain mitochondrial respiration to relieve hypoxia and enhance photodynamic therapy.
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http://dx.doi.org/10.1039/d1cc00544hDOI Listing
April 2021

Color computational ghost imaging based on a generative adversarial network.

Opt Lett 2021 Apr;46(8):1840-1843

A novel, to the best of our knowledge, color computational ghost imaging scheme is presented for the reconstruction of a color object image, which greatly simplifies the experimental setup and shortens the acquisition time. Compared to conventional schemes, it only adopts one digital light projector to project color speckles and one single-pixel detector to receive the light intensity, instead of utilizing three monochromatic paths separately and synthesizing the three branch results. Severe noise and color distortion, which are common in ghost imaging, can be removed by the utilization of a generative adversarial network, because it has advantages in restoring the image's texture details and generating the image's match to a human's subjective feelings over other generative models in deep learning. The final results can perform consistently better visual quality with more realistic and natural textures, even at the low sampling rate of 0.05.
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http://dx.doi.org/10.1364/OL.418628DOI Listing
April 2021

Long non-coding RNA SNHG14 aggravates LPS-induced acute kidney injury through regulating miR-495-3p/HIPK1.

Acta Biochim Biophys Sin (Shanghai) 2021 Apr 15. Epub 2021 Apr 15.

Department of Emergency, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.

Acute kidney injury (AKI) is a complex syndrome with an abrupt decrease of kidney function, which is associated with high morbidity and mortality. Sepsis is the common cause of AKI. Mounting evidence has demonstrated that long non-coding RNAs (lncRNAs) play critical roles in the development and progression of sepsis-induced AKI. In this study, we aimed to illustrate the function and mechanism of lncRNA SNHG14 in lipopolysaccharide (LPS)-induced AKI. We found that SNHG14 was highly expressed in the plasma of sepsis patients with AKI. SNHG14 inhibited cell proliferation and autophagy and promoted cell apoptosis and inflammatory cytokine production in LPS-stimulated HK-2 cells. Functionally, SNHG14 acted as a competing endogenous RNA (ceRNA) to negatively regulate miR-495-3p expression in HK-2 cells. Furthermore, we identified that HIPK1 is a direct target of miR-495-3p in HK-2 cells. We also revealed that the SNHG14/miR-495-3p/HIPK1 interaction network regulated HK-2 cell proliferation, apoptosis, autophagy, and inflammatory cytokine production upon LPS stimulation. In addition, we demonstrated that the SNHG14/miR-495-3p/HIPK1 interaction network regulated the production of inflammatory cytokines (TNF-α, IL-6, and IL-1β) via modulating NF-κB/p65 signaling in LPS-challenged HK-2 cells. In conclusion, our findings suggested a novel therapeutic axis of SNHG14/miR-495-3p/HIPK1 to treat sepsis-induced AKI.
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http://dx.doi.org/10.1093/abbs/gmab034DOI Listing
April 2021

Adverse body measurements are superior to sarcopenia-associated measurements in predicting chronic diseases.

Sci Rep 2021 Apr 8;11(1):7749. Epub 2021 Apr 8.

Healthy Aging Research Center, Chang Gung University, Taoyuan, Taiwan.

Few studies have demonstrated an association of sarcopenia-associated body measurements with chronic diseases through a comprehensive methodology. This study aims to examine the relationship between sarcopenia-associated body measurements and chronic diseases. This is a cohort study. We recruited 316 community dwellers, including 76 patients with sarcopenia and 240 controls, and obtained their body measurements associated with sarcopenia. We collected three-dimensional anthropometric body-surface measurements from 11,158 participants during 2000-2008 and followed up this cohort for 15 years to examine the association of these measurements with the risk of chronic diseases such as hypertension, type 2 diabetes mellitus (T2DM), heart disease, and nephrotic syndrome. Univariate analysis, canonical correlation, and Cox regression analysis were performed to explore the associations. Decreased waist width, upper left arm circumference, and left thigh circumference were significantly associated with sarcopenia. The adverse body measure score (ABMS) was derived by combining significant measurements, namely left upper arm circumference, waist width, and left thigh circumference, and used to predict the risk of hypertension, T2DM, heart diseases, and nephrotic syndrome. A positive association was observed between the ABMS and chronic diseases. Considering the first quartile of the ABMS as a reference, we determined hazard ratios of 2.259, 2.495, 1.332, and 1.595 for hypertension, T2DM, heart disease, and nephrotic syndrome, respectively, in the fourth quartile. Chronic diseases were more strongly associated with the ABMS than with sarcopenia-related body measurements alone. A high ABMS, which includes higher upper arm circumference, higher waist width, and lower thigh circumference, can significantly predict chronic diseases.
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http://dx.doi.org/10.1038/s41598-021-85316-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032743PMC
April 2021

Comparative transcriptome analysis of transcripts of uncertain coding potential in septic myocardial depression.

BMC Cardiovasc Disord 2021 Apr 8;21(1):166. Epub 2021 Apr 8.

Department of Pediatrics, PICU, Shengjing Hospital of China Medical University, No. 36, SanHao Street, Shenyang City, 110004, Liaoning Province, People's Republic of China.

Background: Septic shock with myocardial depression is very common in intensive care units. However, the exact molecular mechanisms underlying sepsis-induced myocardial depression remain unclear. Whether the profiles of transcripts of uncertain coding potential (TUCPs) differ between patients with and without myocardial depression is also unknown. Our study aimed to find expression differences between groups of TUCPs and determine their potential functions in a preclinical model.

Methods: We generated rat models of hypodynamic septic shock induced by lipopolysaccharide. A total of 12 rats were established and left ventricular tissue from each was collected. We performed RNA-seq to identify TUCPs in each sample. Transcripts with an corrected P value of < 0.05 were defined as differentially expressed (DE). We also performed GO terms and KEGG analysis to identify the potential functions of DE TUCPs.

Results: A total of 4,851 TUCPs were identified in heart samples, 85 of which were expressed differently between the sepsis and control groups. Further bioinformatic analyses suggested that TUCPs play important roles in myocardial contraction, energy regulation, and metabolic processes, and are also involved in the regulation of several pathways.

Conclusion: Our results demonstrate that TUCPs both participate in and mediate the pathological process of myocardial depression. Our study improves the understanding of the basic molecular mechanisms underlying myocardial depression from a novel perspective.
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http://dx.doi.org/10.1186/s12872-021-01973-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8028820PMC
April 2021

Normal tissue adjacent to tumor expression profile analysis developed and validated a prognostic model based on Hippo-related genes in hepatocellular carcinoma.

Cancer Med 2021 05 4;10(9):3139-3152. Epub 2021 Apr 4.

Department of Infectious Diseases, The Key Laboratory of Molecular Biology for Infectious Diseases, Chinese Ministry of Education, Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: Hepatocellular carcinoma (HCC) is the most common malignant disease worldwide. Although the diagnosis and treatment of HCC have greatly improved in the recent years, there is still a lack of accurate methods to predict the prognosis of patients. Evidence has shown that Hippo signaling in tissues adjacent to HCC plays a significant role in HCC development. In the present study, we aimed to construct a model based on the expression of Hippo-related genes (HRGs) in tissues adjacent to HCC to predict the prognosis of HCC patients.

Methods: Gene expression data of paired normal tissues adjacent to HCC (PNTAH) and clinical information were obtained from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases. The HRG signature was constructed using four canonical Hippo-related pathways. Univariate Cox regression analysis was used to screen survival-related HRGs. LASSO and multivariate Cox regression analyses were used to construct the prognostic model. The true and false positive rates of the model were confirmed using receiver operating characteristic (ROC) analysis.

Results: The prognostic model was constructed based on the expression levels of five HRGs (NF2, MYC, BIRC3, CSNK1E, and MINK1) in PNTAH. The mortality rate of HCC patients increased as the risk score determined by the model increased. Furthermore, the risk score was found to be an independent risk factor for the survival of patients. ROC analysis showed that the prognostic model had a better predictive value than the other conventional clinical parameters. Moreover, the reliability of the prognostic model was confirmed in TCGA-LIHC cohort. A nomogram was generated to predict patient survival. An exploration of the predictive value of the model in HCC tissues indicated that the model is PNTAH-specific.

Conclusions: We developed and validated a prognostic model based on the expression levels of five HRGs in PNTAH, and this model should be helpful in predicting the prognosis of patients with HCC.
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http://dx.doi.org/10.1002/cam4.3890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085948PMC
May 2021

Cardiac retinoic acid levels decline in heart failure.

JCI Insight 2021 Apr 22;6(8). Epub 2021 Apr 22.

Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Although low circulating levels of the vitamin A metabolite, all-trans retinoic acid (ATRA), are associated with increased risk of cardiovascular events and all-cause mortality, few studies have addressed whether cardiac retinoid levels are altered in the failing heart. Here, we showed that proteomic analyses of human and guinea pig heart failure (HF) were consistent with a decline in resident cardiac ATRA. Quantitation of the retinoids in ventricular myocardium by mass spectrometry revealed 32% and 39% ATRA decreases in guinea pig HF and in patients with idiopathic dilated cardiomyopathy (IDCM), respectively, despite ample reserves of cardiac vitamin A. ATRA (2 mg/kg/d) was sufficient to mitigate cardiac remodeling and prevent functional decline in guinea pig HF. Although cardiac ATRA declined in guinea pig HF and human IDCM, levels of certain retinoid metabolic enzymes diverged. Specifically, high expression of the ATRA-catabolizing enzyme, CYP26A1, in human IDCM could dampen prospects for an ATRA-based therapy. Pertinently, a pan-CYP26 inhibitor, talarozole, blunted the impact of phenylephrine on ATRA decline and hypertrophy in neonatal rat ventricular myocytes. Taken together, we submit that low cardiac ATRA attenuates the expression of critical ATRA-dependent gene programs in HF and that strategies to normalize ATRA metabolism, like CYP26 inhibition, may have therapeutic potential.
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http://dx.doi.org/10.1172/jci.insight.137593DOI Listing
April 2021

Exogenous melatonin positively regulates lignin biosynthesis in Camellia sinensis.

Int J Biol Macromol 2021 May 5;179:485-499. Epub 2021 Mar 5.

Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address:

Melatonin (MT) is a bioactive molecule that can regulate various developmental processes. Changes in lignin content play important roles in plant growth and development. Herein, quantitative analysis and histochemical staining showed that lignin content significantly increased over time, and melatonin treatment triggered the lignification at 8 and 16 d in tea leaves. The POD activity participated in lignin formation had also been significantly improved. The effect of melatonin on the increase of lignin content was attenuation over time. Sequencing results based on transcriptome at 8 and 16 d showed that 5273 and 3019 differentially expressed genes (DEGs) were identified in CK1 vs. MT1 and CK2 vs. MT2, respectively. A total of 67 DEGs were annotated to lignin biosynthesis, and 38 and 9 genes were significantly up-regulated under melatonin treatment, respectively. Some transcription factor genes such as MYB were also identified among the two pairwise comparisons, which might relate to lignin metabolism. Melatonin increased the degree of lignification in tea leaves by modifying the enzyme genes expression involved in lignin synthesis pathway. These results provide a reference for further study on the molecular mechanism of the dynamic changes of lignin content induced by melatonin treatment in tea plants.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.03.025DOI Listing
May 2021

Visualization and Analysis in the Field of Pan-Cancer Studies and Its Application in Breast Cancer Treatment.

Front Med (Lausanne) 2021 17;8:635035. Epub 2021 Feb 17.

School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Although all cancers are molecularly distinct, many share common driver mutations. Pan-cancer analysis, utilizes next-generation sequencing (NGS), pan-cancer model systems, and pan-cancer projects such as The Cancer Genome Atlas (TCGA), to assess frequently mutated genes and other genomic abnormalities that are common among many cancer types, regardless of the tumor origin, providing new directions for tumor biology research. However, there is currently no study that has objectively analyzed the results of pan-cancer studies on cancer biology. For this study, 999 articles on pan-cancer published from 2006 to 2020 were obtained from the Scopus database, and bibliometric methods were used to analyze citations, international cooperation, co-authorship and keyword co-occurrence clusters. Furthermore, we also focused on and summarized the application of pan-cancer in breast cancer. Our result shows that the pan-cancer studies were first published in 2006 and entered a period of rapid development after 2013. So far, 86 countries have carried out international cooperation in sharing research. Researchers form the United States and Canada have published the most articles and have made the most extensive contribution to this field, respectively. Through author keyword analysis of the 999 articles, TCGA, biomarkers, NGS, immunotherapy, DNA methylation, prognosis, and several other keywords appear frequently, and these terms are hot spots in pan-cancer studies. There are four subtypes of breast cancer (luminalA, luminalB, HER2, and basal-like) according to pan-cancer analysis of breast cancer. Meanwhile, it was found that breast cancer has genetic similarity to pan-gynecological cancers, such as ovarian cancer, which indicates related etiology and possibly similar treatments. Collectively, with the emergence of new detection methods, new cancer databases, and the involvement of more researchers, pan-cancer analyses will play a greater role in cancer biology research.
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http://dx.doi.org/10.3389/fmed.2021.635035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926202PMC
February 2021

Acute changes to breast milk composition following consumption of high-fat and high-sugar meals.

Matern Child Nutr 2021 Mar 3:e13168. Epub 2021 Mar 3.

School of Biosciences, University of Nottingham, Sutton Bonington, Loughborough, UK.

Breast milk composition is influenced by habitual diet, yet little is known about the short-term effects of changes in maternal diet on breast milk macronutrient concentrations. Our aim was to determine the acute effect of increased consumption of sugar/fat on breast milk protein, lactose and lipids. Exclusively breastfeeding women (n = 9) were provided with a control, higher fat (+28 g fat) and higher sugar (+66 g sugar) diet over three separate days at least 1 week apart. Hourly breast milk samples were collected concurrently for the analysis of triglycerides, cholesterol, protein, and lactose concentrations. Breast milk triglycerides increased significantly following both the higher fat and sugar diet with a greater response to the higher sugar compared to control diet (mean differences of 3.05 g/dL ± 0.39 and 13.8 g/dL ± 0.39 in higher fat and sugar diets, respectively [P < 0.001]). Breast milk cholesterol concentrations increased most in response to the higher sugar diet (0.07 g/dL ± 0.005) compared to the control (0.04 g/dL) and the higher fat diet (0.05 g/dL) P < 0.005. Breast milk triglyceride and lactose concentrations increased (P < 0.001, P = 0.006), whereas protein decreased (p = 0.05) in response to the higher fat diet compared to the control. Independent of diet, there were significant variations in breast milk composition over the day; triglycerides and cholesterol concentrations were higher at end of day (P < 0.001), whereas protein and lactose concentrations peaked at Hour 10 (of 12) (P < 0.001). In conclusion, controlled short-term feeding to increase daily sugar/fat consumption altered breast milk triglycerides, cholesterol, protein and lactose. The variations observed in breast milk protein and lactose across the 12 h period is suggestive of a circadian rhythm.
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http://dx.doi.org/10.1111/mcn.13168DOI Listing
March 2021

Impact of capsaicin on aroma release and perception from flavoured solutions.

Lebensm Wiss Technol 2021 Mar;138:110613

Division of Food, Nutrition and Dietetics, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.

Capsaicin is the main component in chilli pepper, which contributes to the spiciness of the food. However, the role of capsaicin on aroma perception has been controversial in the literature. This is the first study exploring the impact of capsaicin on aroma release and perception simultaneously. Flavoured solutions with 3-methylbutanal (nutty note) were made with or without 5 mg/L capsaicin. Real-time APCI-MS analysis was applied to investigate in-nose aroma release during and after consumption of the solutions, and sensory tests were simultaneously conducted to reveal any temporal perception changes over 60 s. The results from 15 participants with triplicates indicated that capsaicin had no significant impact on aroma concentration from aqueous solutions, but the aroma perception rating was significantly higher (p < 0.0001), increasing by 45%. Capsaicin also enhanced average saliva flow by 92% (p < 0.0001), and lower saliva flow participants were found to have lower spiciness and aroma ratings.
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http://dx.doi.org/10.1016/j.lwt.2020.110613DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829613PMC
March 2021

Changes in structure and allergenicity of shrimp tropomyosin by dietary polyphenols treatment.

Food Res Int 2021 Feb 15;140:109997. Epub 2020 Dec 15.

School of Public Health, Health Science Center, Shenzhen University, Shenzhen, Guangdong Province 518060, China. Electronic address:

Here, the potential allergenicity of shrimp tropomyosin (TM) after conjugation with chlorogenic acid (CA) and (-)-epigallo-catechin 3-gallate (EGCG) was assessed. Conformational structures of TM-polyphenol complexes were detected using SDS-PAGE, circular dichroism (CD), and fluorescence. Potential allergenicity was assessed by immunological methods, a rat basophil leukemia cell model (RBL-2H3), and in vivo assays. Indirect ELISA showed that TM-polyphenol complexes caused a conformational change to TM structure, with decreased IgG/IgE binding capacity significantly fewer inflammatory mediators were released with EGCG-TM and CA-TM in a mediator-releasing RBL-2H3 cell line. Mice model showed low allergenicity to serum levels of TM-specific antibody and T-cell cytokine production. EGCG-TM and CA-TM might reduce the potential allergenicity of shrimp TM, which could be used to produce hypoallergenic food in the food industry.
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http://dx.doi.org/10.1016/j.foodres.2020.109997DOI Listing
February 2021

Bayesian biclustering for microbial metagenomic sequencing data via multinomial matrix factorization.

Biostatistics 2021 Feb 26. Epub 2021 Feb 26.

Department of Nutrition and Food Science, Texas A&M University, College Station, TX, USA.

High-throughput sequencing technology provides unprecedented opportunities to quantitatively explore human gut microbiome and its relation to diseases. Microbiome data are compositional, sparse, noisy, and heterogeneous, which pose serious challenges for statistical modeling. We propose an identifiable Bayesian multinomial matrix factorization model to infer overlapping clusters on both microbes and hosts. The proposed method represents the observed over-dispersed zero-inflated count matrix as Dirichlet-multinomial mixtures on which latent cluster structures are built hierarchically. Under the Bayesian framework, the number of clusters is automatically determined and available information from a taxonomic rank tree of microbes is naturally incorporated, which greatly improves the interpretability of our findings. We demonstrate the utility of the proposed approach by comparing to alternative methods in simulations. An application to a human gut microbiome data set involving patients with inflammatory bowel disease reveals interesting clusters, which contain bacteria families Bacteroidaceae, Bifidobacteriaceae, Enterobacteriaceae, Fusobacteriaceae, Lachnospiraceae, Ruminococcaceae, Pasteurellaceae, and Porphyromonadaceae that are known to be related to the inflammatory bowel disease and its subtypes according to biological literature. Our findings can help generate potential hypotheses for future investigation of the heterogeneity of the human gut microbiome.
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http://dx.doi.org/10.1093/biostatistics/kxab002DOI Listing
February 2021

Physiological and TMT-labeled proteomic analyses reveal important roles of sugar and secondary metabolism in Citrus junos under cold stress.

J Proteomics 2021 04 11;237:104145. Epub 2021 Feb 11.

Chenggu Fruit Industry Technical Guidance Station, Chenggu 723200, Shaanxi, China.

Citrus junos is a widely used citrus grafting rootstock in china because of its excellent tolerance to cold stress. However, the physiological and molecular mechanisms underlying this process remain unknown. In this study, physiological and tandem mass tag-based proteomic analyses were performed to elucidate the mechanism of the Citrus junos response to cold stress. Physiological data showed that severe cold stress decreased photosynthetic parameters and caused cell membrane damage and membrane lipid peroxidation in Citrus junos leaves compared to the control. A total of 6, 678 distinct proteins species were identified, and 413 proteins species were significantly differentially accumulated in the leaves of Citrus junos seedling after cold stress. Bioinformatics analysis revealed that the differentially abundance protein species mainly related to the starch and sucrose metabolism, secondary metabolites biosynthesis and phenylpropanoid biosynthesis in the leaves of Citrus junos seedling after cold stress. Further physiological assays showed that the contents of soluble starch, fructose, glucose and phenols were significantly increased in Citrus junos leaves after cold stress. Collectively, our data reveals that sugar and secondary metabolism could play important roles in Citrus junos in response to cold stress.
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http://dx.doi.org/10.1016/j.jprot.2021.104145DOI Listing
April 2021

Protective Role of Sulodexide on Renal Injury Induced by Limb Ischemia-Reperfusion.

Evid Based Complement Alternat Med 2021 30;2021:6629718. Epub 2021 Jan 30.

Department of Vascular Surgery, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.

Background: Though widely known as a potent antithrombin agent with protective effects on the kidney and other remote organs, it is currently ambiguous when it comes to sulodexide's function on ischemia-reperfusion (I/R) injury. With this research, we pursued to further explore how sulodexide exerts its influence on limb I/R injury, in which deleterious effects on the kidney were what we primarily focused on.

Methods: We randomized twenty-four C57BL/6 male rats into three groups, namely, sham operation group (control group), I/R group, and sulodexide pretreatment group. Hematoxylin and eosin staining was applied for discovery of renal histological changes. Serum creatinine (Cr) and serum urea nitrogen (BUN) were measured. Apoptotic parameters were detected by the TdT-mediated dUTP Nick-End Labeling method. To what extent and levels that antiapoptotic and proapoptotic proteins were expressed could be sensitively revealed by immunohistochemistry assay. Lipid peroxidation product propylene glycol and inflammatory factors were examined by enzyme-linked immunosorbent assay. Additionally, an extracorporeal hypoxia-reoxygenation (H/R) model of human renal proximal tubule epithelial HK2 cells was established. Our targets lay in cell proliferation and apoptosis, and we used western blotting to reflect apoptosis-related gene expression.

Results: The levels of serum BUN, Cr, and inflammatory factors in sulodexide-intervened rats manifested significant reduction when compared with the I/R group. Also, sulodexide could protect the kidney from histological changes and could effectively inhibit intraparenchymal apoptosis. Furthermore, adding 2 l/mL or 5 l/mL of sulodexide to H/R model cells in vitro gave rise to significant restoration of the degenerative proliferation capacity of the HK2 cells following H/R injury and late cellular apoptosis experienced dramatic reduction versus the H/R group. When treated with 5 l/mL of sulodexide at a dose of 10 mg/kg, the levels of the antiapoptotic proteins were increased, while the proapoptotic proteins showed opposite trends. Notable escalation on antiapoptotic protein expression level, in contrast with the opposite trends exhibited in proapoptotic proteins, was observed with 5 l/mL sulodexide pretreatment with the dosage being 10 mg/kg.

Conclusion: Sulodexide can protect against kidney damage caused by I/R injury of the lower limbs by enhancing cell proliferation, inhibiting apoptosis, reducing inflammatory reactions, and scavenging oxygen free radicals.
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http://dx.doi.org/10.1155/2021/6629718DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867463PMC
January 2021

MCU Overexpression Rescues Inotropy and Reverses Heart Failure by Reducing SR Ca Leak.

Circ Res 2021 Apr 1;128(8):1191-1204. Epub 2021 Feb 1.

Division of Cardiology, Department of Medicine, The Johns Hopkins University, Baltimore, MD.

[Figure: see text].
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http://dx.doi.org/10.1161/CIRCRESAHA.120.318562DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049980PMC
April 2021

Consensus Monte Carlo for Random Subsets using Shared Anchors.

J Comput Graph Stat 2020 15;29(4):703-714. Epub 2020 Apr 15.

Department of Mathematics, The University of Texas at Austin.

We present a consensus Monte Carlo algorithm that scales existing Bayesian nonparametric models for clustering and feature allocation to big data. The algorithm is valid for any prior on random subsets such as partitions and latent feature allocation, under essentially any sampling model. Motivated by three case studies, we focus on clustering induced by a Dirichlet process mixture sampling model, inference under an Indian buffet process prior with a binomial sampling model, and with a categorical sampling model. We assess the proposed algorithm with simulation studies and show results for inference with three datasets: an MNIST image dataset, a dataset of pancreatic cancer mutations, and a large set of electronic health records (EHR). Supplementary materials for this article are available online.
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http://dx.doi.org/10.1080/10618600.2020.1737085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810350PMC
April 2020

The role of sexual compulsivity in unprotected intercourse among STI patients in Shanghai, China.

BMC Public Health 2021 01 15;21(1):141. Epub 2021 Jan 15.

School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.

Background: Sexual compulsivity (SC) and its relationship with unprotected intercourse (UI) have long been an intriguing topic, but its existential meaning in the management of public health or, more precisely, sexually transmitted infections (STIs) has rarely been studied to date. This study examines whether SC plays a role in UI among sexually active STI patients.

Method: A cross-sectional study was conducted in two sexual transmitted disease (STD) clinicals of Shanghai Skin Diseases Hospital in Shanghai. Totally 664 sexually active STI patients were included.

Results: The ages of the 664 participants ranged from 18 to 76 years, with 58.73% between 26 and 40 years old. 449 (191 male and 258 female) reported had UI during the past 6 months. Although the only statistically significant difference (p < 0.01) was in relation to UI with a casual sexual partner, the difference between male/female and regular/casual sexual partners remained evident.

Conclusions: SC is evidently a potential predictor of UI with a casual sexual partner in male STI patients, while the use of condoms is more likely to be affected by other factors. In addition to general sexual education, counseling interventions should be provided by health institutions, and specific intervention methods targeting gender and sexual partners should be considered.
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http://dx.doi.org/10.1186/s12889-021-10186-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809841PMC
January 2021

FOXG1 Directly Suppresses Wnt5a During the Development of the Hippocampus.

Neurosci Bull 2021 Mar 3;37(3):298-310. Epub 2021 Jan 3.

Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, School of Medicine, Southeast University, Nanjing, 210009, China.

The Wnt signaling pathway plays key roles in various developmental processes. Wnt5a, which activates the non-canonical pathway, has been shown to be particularly important for axon guidance and outgrowth as well as dendrite morphogenesis. However, the mechanism underlying the regulation of Wnt5a remains unclear. Here, through conditional disruption of Foxg1 in hippocampal progenitors and postmitotic neurons achieved by crossing Foxg1 with Emx1-Cre and Nex-Cre, respectively, we found that Wnt5a rather than Wnt3a/Wnt2b was markedly upregulated. Overexpression of Foxg1 had the opposite effects along with decreased dendritic complexity and reduced mossy fibers in the hippocampus. We further demonstrated that FOXG1 directly repressed Wnt5a by binding to its promoter and one enhancer site. These results expand our knowledge of the interaction between Foxg1 and Wnt signaling and help elucidate the mechanisms underlying hippocampal development.
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http://dx.doi.org/10.1007/s12264-020-00618-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954983PMC
March 2021

Probing the effect of aroma compounds on the hydrodynamic properties of mucin glycoproteins.

Eur Biophys J 2020 Dec 13;49(8):799-808. Epub 2020 Nov 13.

Division of Food, Nutrition and Dietetics, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Leicestershire, UK.

Aroma compounds are diverse low molecular weight organic molecules responsible for the flavour of food, medicines or cosmetics. Natural and artificial aroma compounds are manufactured and used by the industry to enhance the flavour and fragrance of products. While the low concentrations of aroma compounds present in food may leave no effect on the structural integrity of the mucosa, the effect of concentrated aroma volatiles is not well understood. At high concentrations, like those found in some flavoured products such as e-cigarettes, some aroma compounds are suggested to elicit a certain degree of change in the mucin glycoprotein network, depending on their functional group. These effects are particularly associated with carbonyl compounds such as aldehydes and ketones, but also phenols which may interact with mucin and other glycoproteins through other interaction mechanisms. This study demonstrates the formation of such interactions in vitro through the use of molecular hydrodynamics. Sedimentation velocity studies reveal that the strength of the carbonyl compound interaction is influenced by compound hydrophobicity, in which the more reactive short chain compounds show the largest increase in mucin-aroma sedimentation coefficients. By contrast, the presence of groups that increases the steric hindrance of the carbonyl group, such as ketones, produced a milder effect. The interaction effects were further demonstrated for hexanal using size exclusion chromatography light scattering (SEC-MALS) and intrinsic viscosity. In addition, phenolic aroma compounds were identified to reduce the sedimentation coefficient of mucin, which is consistent with interactions in the non-glycosylated mucin region.
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http://dx.doi.org/10.1007/s00249-020-01475-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701130PMC
December 2020

Scalable Bayesian Nonparametric Clustering and Classification.

J Comput Graph Stat 2020 19;29(1):53-65. Epub 2019 Jul 19.

Department of Public Health Sciences, The University of Chicago.

We develop a scalable multi-step Monte Carlo algorithm for inference under a large class of nonparametric Bayesian models for clustering and classification. Each step is "embarrassingly parallel" and can be implemented using the same Markov chain Monte Carlo sampler. The simplicity and generality of our approach makes inference for a wide range of Bayesian nonparametric mixture models applicable to large datasets. Specifically, we apply the approach to inference under a product partition model with regression on covariates. We show results for inference with two motivating data sets: a large set of electronic health records (EHR) and a bank telemarketing dataset. We find interesting clusters and competitive classification performance relative to other widely used competing classifiers. Supplementary materials for this article are available online.
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http://dx.doi.org/10.1080/10618600.2019.1624366DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7518195PMC
July 2019

HS Regulation of Metabolism in Cucumber in Response to Salt-Stress Through Transcriptome and Proteome Analysis.

Front Plant Sci 2020 19;11:1283. Epub 2020 Aug 19.

School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, China.

In a previous study, we found that HS alleviates salinity stress in cucumber by maintaining the Na/K balance and by regulating HS metabolism and the oxidative stress response. However, little is known about the molecular mechanisms behind HS-regulated salt-stress tolerance in cucumber. Here, an integrated transcriptomic and proteomic analysis based on RNA-seq and 2-DE was used to investigate the global mechanism underlying HS-regulated salt-stress tolerance. In total, 11,761 differentially expressed genes (DEGs) and 61 differentially expressed proteins (DEPs) were identified. Analysis of the pathways associated with the DEGs showed that salt stress enriched expression of genes in primary and energy metabolism, such as photosynthesis, carbon metabolism and biosynthesis of amino acids. Application of HS significantly decreased these DEGs but enriched DEGs related to plant-pathogen interaction, sulfur-containing metabolism, cell defense, and signal transduction pathways. Notably, changes related to sulfur-containing metabolism and cell defense were also observed through proteome analysis, such as , , , and . We present the first global analysis of the mechanism underlying HS regulation of salt-stress tolerance in cucumber through tracking changes in the expression of specific proteins and genes.
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http://dx.doi.org/10.3389/fpls.2020.01283DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466724PMC
August 2020

Image-guided percutaneous microwave ablation of early-stage non-small cell lung cancer.

Authors:
Yang Ni Hui Xu Xin Ye

Asia Pac J Clin Oncol 2020 Dec 24;16(6):320-325. Epub 2020 Sep 24.

Department of Oncology, The First Affiliated Hospital of Shandong First Medical University, Jinan, Shandong Province, China.

Although surgical lobectomy with systematic mediastinal lymph node evaluation is considered as the "gold standard" for management of early stage non-small cell lung cancer (NSCLC), image-guided percutaneous thermal ablation has been increasingly used for medically inoperable patients. Radiofrequency ablation (RFA) is a research-based technique that has the most studies for medically inoperable early-stage NSCLC. Other thermal ablation techniques used to treat pulmonary tumors include microwave ablation (MWA), cryoablation and laser ablation. MWA has several advantages over RFA including reduced procedural time, reduced heat-sink effect, large ablation zones, decreased susceptibility to tissue impedance, and simultaneous use of multiple antennae. This review article highlights the most relevant updates of MWA for the treatment of early-stage NSCLC, including mechanism of action, clinical outcomes, potential complications, the existing technique problems and future directions.
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http://dx.doi.org/10.1111/ajco.13419DOI Listing
December 2020

Role of Glucuronidation Pathway in Quetiapine Metabolism: An Drug-Drug Interaction Study between Quetiapine and Probenecid.

Saudi J Med Med Sci 2020 Sep-Dec;8(3):196-200. Epub 2020 Aug 20.

Department of Clinical Pharmacy, Wuhan Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Uridine 5'-diphospho-glucuronosyltransferase (UGT) enzymes play a significant role in the metabolism of quetiapine, and coadministration with a UGT inhibitor/inducer drug may change its pharmacokinetic profile.

Objective: The objective of this study was to assess the impact of probenecid, a UGT enzyme inhibitor, on the pharmacokinetic profile of quetiapine.

Materials And Methods: Twelve treatment-naïve, 7-week-old male Sprague-Dawley rats (weighting 161 ± 22 g) were randomly and equally divided into control, quetiapine-alone and quetiapine plus probenecid groups. The quetiapine plus probenecid group received a single oral dose of probenecid (50 mg/kg) followed by 50 mg/kg of quetiapine; the quetiapine-alone group only received 50 mg/kg of quetiapine. Blood samples (0.2 ml) were collected from all rats after 0, 0.25, 0.5, 1, 2, 4, 6, 8, 10, 12 and 24 h of the drug administration in heparinized tubes. The pre-established liquid chromatography-mass spectrometry method was utilized to ascertain the plasma concentration of quetiapine and the control group was used to prepare the controlled standard.

Results: Significant pharmacokinetic differences were observed between the quetiapine-alone and quetiapine plus probenecid groups in terms of C (392 ± 209 vs. 1323 ± 343 ug/L, respectively, = 0.004), AUC ( = 0.04) and T ( = 0.004). Further, in the combined drug group, there was a decrease in drug clearance (CL/F) (from 27 ± 11 to 16 ± 3 L/h/kg; = 0.005) and an increase in the volume of distribution (Vd) ( = 0.01), but there was no significant difference between both groups in terms of half-lives ( = 0.27). No significant within-group variability of pharmacokinetic parameters was observed ( = 0.25).

Conclusion: The results of this animal study suggest that glucuronidation by UGT enzyme system may also play an important role in quetiapine metabolism, which, if proven in future human studies, would imply that the bioavailability and pharmacokinetic parameters of quetiapine may require alterations when co-administered with probenecid to avoid development of quetiapine toxicity.
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http://dx.doi.org/10.4103/sjmms.sjmms_441_19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485652PMC
August 2020