Publications by authors named "Yang Luo"

616 Publications

Evaluation of Cadmium Transfer from Soil to the Human Body Through Maize Consumption in a Cadmium Anomaly Area of Southwestern China.

Environ Toxicol Chem 2021 Jul 21. Epub 2021 Jul 21.

College of Resources and Environmental Engineering, Guizhou University, Guiyang, 550025, China.

Evaluating the bioavailability, bioaccessibility and transferability of cadmium (Cd) in soil-grain-human systems is essential in areas with a Cd anomaly in the karst region of southwestern China. In the present study, the main controlling factors and prediction models for Cd transfer in a soil-grain-human systems were investigated in a typical area where natural processes and anthropogenic activities interact in the karst region of southwestern China. The environmental availability of Cd (diethylenetriaminepentaacetic acid- [DTPA] and CaCl -extractable Cd [Cd ]) in the soil varies significantly because of the diversity of soil properties. However, Cd concentrations in the maize grain were significantly related only to the Cd concentrations in the soil (r = 0.595, p < 0.01), indicating that soil Cd is a good indicator for evaluating Cd uptake by maize grain. Of all the measured soil properties, the soil cation exchange capacity (CEC) and the soil calcium (Ca ) were the most important factor influencing Cd accumulation in the soil-maize grain system. A transfer model combining Cd , soil CEC and Ca was sufficiently reliable for predicting Cd accumulation in the maize grain (R = 0.505 ). Although there is more room for improvement regarding the prediction performance of the chain model combining soil Cd with Ca to predict bioaccessible Cd concentration in the maize grain (R = 0.344 for the gastric phase and R = 0.356 for the gastrointestinal phase), our findings provide a useful reference to further explore a model that can be used for a relatively rapid and reliable estimation of dietary Cd exposure for specific regions prior to crop harvest. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/etc.5171DOI Listing
July 2021

A Dendritic Cells-Targeting Nano-Vaccine by Coupling Polylactic-Co-Glycolic Acid-Encapsulated Allergen with Mannan Induces Regulatory T Cells.

Int Arch Allergy Immunol 2021 Jul 21:1-11. Epub 2021 Jul 21.

Department of Allergy and Rheumatology, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China.

Background: The efficacy of allergen-specific immunotherapy (AIT) is mainly depended on the tolerogenic immune responses elicited. Properly conjugated nano-vaccine has the advantages of both specific targeting and continuous and on-demand release of allergen.

Objectives: The aim of this study is to investigate the effects of a dendritic cells (DCs)-targeting nano-vaccine for AIT.

Methods: The nano-vaccine was produced by coupling polylactic-co-glycolic acid (PLGA)-encapsulated ovalbumin (OVA) with mannan. Allergen capture, human monocytes-derived DCs (hMoDCs) activation, and T cells responses were assessed by flow cytometry, confocal microscopy, quantitative real-time PCR, ELISA, and Cytometric Bead Array. Balb/c mice were immunized with the nano-vaccines, and the immune responses were analyzed.

Results: OVA-PLGA nanoparticle (NP) displayed favorable safety profile. OVA-mannan-PLGA NP was captured more efficiently by hMoDCs than OVA-PLGA NP, which was mediated mainly through DC-specific intercellular adhesion molecule 3-grabbing nonintegrin. A tolerogenic phenotype of hMoDCs was induced by OVA-mannan-PLGA NP, but not OVA-PLGA NP, and increased number of regulatory T (Treg) cells was generated subsequently in in vitro coculture. Immunization of Balb/c mice with OVA-mannan-PLGA NP resulted in lower serum level of OVA-specific immunoglobulins and less production of pro-inflammatory cytokines in splenocytes culture than the mice immunized with OVA-PLAG NP, PLGA NP, or OVA, while the number of splenic Treg cells was higher in OVA-mannan-PLGA group than in other groups. Moreover, preimmunization with OVA-mannan-PLGA NP significantly inhibited the Th2 immune response induced by OVA sensitization.

Conclusions: The biocompatible PLGA-encapsulated OVA coupling with mannan has augmented ability for tolerance induction and could be developed as a novel vaccine for AIT.
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http://dx.doi.org/10.1159/000512872DOI Listing
July 2021

Does Listening to Music Improve Pain Perception and Anxiety in Patients Undergoing Cystoscopy: A Meta-Analysis.

Front Surg 2021 28;8:689782. Epub 2021 Jun 28.

Department of Urology, West China Fourth Hospital of Sichuan University, Chengdu, China.

To identify the effect of music on outpatient-based cystoscopy. We systematically reviewed the effect of music on all reported outpatient for cystoscopy and extracted data from randomized trials from inception to February 3, 2021, with no language restrictions. The analysis was completed STATA version 14.2. A total of 27 studies were initially identified, and 6 articles containing 639 patients were included in the final analysis. In terms of post-procedural pain perception, a pooled analysis of 6 articles containing 639 patients showed that music seems to improve discomfort in patients who undergo cystoscopy (WMD: -1.72; 95%CI: -2.37 to -1.07). This improvement remained consistent in patients undergoing flexible cystoscopy (FC) (WMD: -1.18; 95% CI: -1.39 to -0.98) and rigid cystoscopy (RC) (WMD: -2.56; 95% CI: -3.64 to -1.48). The music group also had less post-procedural anxiety than those in no music group during cystoscopy (WMD: -13.33; 95% CI: -21.61 to -5.06), which was in accordance with the result of FC (WMD: -4.82; 95% CI: -6.38 to -3.26) than RC (WMD: -26.05; 95% CI: -56.13 to 4.04). Besides, we detected a significantly lower post-procedural heart rate (HR) in the music group than no music group during cystoscopy (WMD: -4.04; 95% CI: -5.38 to -2.71), which is similar to the results of subgroup analysis for FC (WMD: -3.77; 95% CI: -5.84 to -1.70) and RC (WMD: -4.24; 95% CI: -5.98 to -2.50). A pooled analysis of three trials indicated that patients in the music group had significantly higher post-operative satisfaction visual analog scale (VAS) scores than those in the no-music group during RC. However, there was no significant difference between the music group and no music group regarding post-procedural systolic pressures (SPs) during cystoscopy (WMD: -3.08; 95% CI: -8.64 to 2.49). For male patients undergoing cystoscopy, the music seemed to exert a similar effect on decreasing anxiety and pain, and it might serve as a useful adjunct to increase procedural satisfaction. These findings indicate that listening to music contributes to the improvement of pain perception, HR, and anxiety feeling during cystoscopy, especially for male patients undergoing RC. Music might serve as a simple, inexpensive, and effective adjunct to sedation during cystoscopy.
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http://dx.doi.org/10.3389/fsurg.2021.689782DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273256PMC
June 2021

Enhances Resistance to Cisplatin via Facilitating Exosomes-mediated Macrophage M2 Polarization in Non-small Cell Lung Cancer.

Immunol Invest 2021 Jul 12:1-14. Epub 2021 Jul 12.

Department of Oncology, Fujian Medical University Union Hospital, Fuzhou, Fujian, China.

Phosphoribosyl pyrophosphate synthetases 2 () is reported as an oncogene in various cancers. However, the role of in cisplatin (DDP) resistance of non-small cell lung cancer (NSCLC) remains unclear. The present study aimed to explore the effect of in DDP resistance of NSCLC. mRNA expression levels of genes were detected by RT-PCR. Enzyme-linked immunosorbent assay (ELISA) and Western blot were used to detect protein expression levels. Cell viability was determined by the MTT assay and colony formation assay. Cell apoptosis was detected using nucleosome ELISA assay and caspase-3 activity assay. silencing was achieved using siRNA transfection. Exosomes of cultured cells were isolated through ultracentrifugation. Elevated was correlated with DDP resistance and poor prognosis in NSCLC patients. silencing enhanced sensitivity of DDP-resistant cells to DDP treatment. NSCLC cell-derived exosome induced M2 macrophage polarization. was enriched in the exosomes of NSCLC cells. Exosomal PRPS2 mediated M2 macrophage polarization to promote DDP resistance of NSCLC cells. In conclusion, potentiates resistance to DDP by promoting exosome-mediated macrophage M2 polarization in NSCLC.
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http://dx.doi.org/10.1080/08820139.2021.1952217DOI Listing
July 2021

Utilization and associated factors of reproductive health services among 20-39-year-old women in rural China: a cross-sectional study.

Reprod Health 2021 Jun 27;18(1):134. Epub 2021 Jun 27.

Xiang Ya Nursing School, Central South University, Changsha, China.

Background: The use of reproductive health (RH) services is important to promote RH. However, little is known about RH services in rural areas, especially in low- and middle-income countries. China is the most populous country in the world, and 40.4% of its population is rural. Our study determined the utilization of and factors associated with RH services in rural China.

Methods: A cross-sectional study of 978 20- to 39-year-old women was performed in four villages of four cities in Hunan Province. A researcher-created structured questionnaire was used to collect the data. The data were entered into EpiData v3.0 and analysed using SPSS v18.0. Statistical significance was defined as a two-sided P-value of less than 0.05. Descriptive statistics were used to examine the socio-demographic factors and the use of RH services by the sample population. Chi-square tests were used to assess associations between categorical variables. Logistic regression analyses were performed to examine factors that correlated with the use of RH services.

Results: The top three services used were antenatal examinations (90.2%), postpartum visits (73.0%) and free folic acid supplements (71.6%). Age, monthly household income, employment, spousal education level, and artificial abortion history were associated with RH service utilization (P < 0.05). The most desired RH service was cervical/breast cancer prevention services (58.9%). The most preferred method participants used to obtain information on RH services was the internet.

Conclusions: The utilization rate for RH services in rural China needs improvement. Future efforts should target high-risk populations of women by providing them with RH-related information and cultivating positive attitudes towards RH services.
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http://dx.doi.org/10.1186/s12978-021-01182-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237466PMC
June 2021

Prostatic fluid exosome-mediated microRNA-155 promotes the pathogenesis of type IIIA chronic prostatitis.

Transl Androl Urol 2021 May;10(5):1976-1987

Department of Urology, Southwest Hospital, The First Affiliated Hospital of Third Military Medical University (Army Medical University), Chongqing, China.

Background: The latest research has shown that exosomes play an important role in cell-to-cell communication and are closely related to the occurrence of many chronic inflammatory diseases. However, no studies have clarified whether exosomes are involved in the pathogenesis of aseptic inflammation, type IIIA chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS-A). This study aimed to explore the relationship between prostatic fluid exosomes and CP/CPPS-A and reveal new pathogenesis.

Methods: Our group collected prostatic fluid samples from CP/CPPS-A patients and normal adult men. Electron microscope, quantitative PCR (qPCR), Western Blot, nanoparticle tracking analysis, hematoxylin-and-eosin (HE) staining, immunofluorescence staining and miRNA-155 functional analysis were used to verify the role of exosomes in CP/CPPS-A and .

Results: Exosomes were abundantly enriched in the prostatic fluid of CP/CPPS-A patients and selectively overloaded with microRNA-155 (). These exosomes were taken up by prostatic stromal cells in large quantities. They activated interleukin (IL)-8 and tumor necrosis factor-alpha (TNF-α) expression , and the integrity of the exosomes' plasma membrane is a necessary condition for information transmission by exosomes. In experiments, histological results showed that prostatic fluid exosomes induced prostatitis in rats. Also, immunofluorescence staining showed excessive activation of IL-8, TNF-α, and inducible nitric oxide synthase (iNOS).

Conclusions: Exosomes in the prostatic fluid and the contained therein were may be involved with the pathogenesis of CP/CPPS-A.
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http://dx.doi.org/10.21037/tau-21-139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185664PMC
May 2021

New frontiers against sorafenib resistance in renal cell carcinoma: From molecular mechanisms to predictive biomarkers.

Pharmacol Res 2021 Jun 15;170:105732. Epub 2021 Jun 15.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu 610041, PR China. Electronic address:

Renal cell carcinoma (RCC) is a highly vascularized tumor and prone to distant metastasis. Sorafenib is the first targeted multikinase inhibitor and first-line chemical drug approved for RCC therapy. In fact, only a small number of RCC patients benefit significantly from sorafenib treatment, while the growing prevalence of sorafenib resistance has become a major obstacle for drug therapy effectivity of sorafenib. The molecular mechanisms of sorafenib resistance in RCC are not completely understood by now. Herein, we comprehensively summarize the underlying mechanisms of sorafenib resistance and molecular biomarkers for predicting sorafenib responsiveness. Moreover, we outline strategies suitable for overcoming sorafenib resistance and prospect potential approaches for identifying biomarkers associated with sorafenib resistance in RCC, which contributes to guide individualized and precision drug therapy.
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http://dx.doi.org/10.1016/j.phrs.2021.105732DOI Listing
June 2021

N6-Methyladenosine-Related RNA Signature Predicting the Prognosis of Ovarian Cancer

Recent Pat Anticancer Drug Discov 2021 06 15. Epub 2021 Jun 15.

The Research Center for Medical Genomics, School of Life Sciences, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang City, 110122, Liaoning, China

Background: N6-Methyladenosine (m6A) RNA methylation is the most universal mRNA modification in eukaryotic cells. M6A mRNA modification affects almost every phases of RNA processing, including splicing, decay, export, translation and expression. Several patents have reported the application of m6A mRNA modification in cancer diagnosis and treatment. Ovarian cancer is the leading cause of death among all gynecological cancers. It is urgent to identify new biomarkers for early diagnosis and prognosis of ovarian cancer.

Objective: In the current study, we aimed to evaluate the m6A RNA methylation regulators and m6A related genes and establish a new gene signature panel for prognosis of ovarian cancer.

Method: We downloaded the Mutations data, FPKM data and corresponding clinical information of 373 patients with ovarian cancer (OC) from the TCGA database. We performed LASSO regression analysis and multivariate cox regression analysis to develop a risk-identifying gene signature panel.

Results: A total of 317 candidate m6A RNA methylation related genes were obtained. Finally, 12 -genes (WTAP, LGR6, ZC2HC1A, SLC4A8, AP2A1, NRAS, CUX1, HDAC1, CD79A, ACE2, FLG2 and LRFN1) were selected to establish the signature panel. We analyzed the genetic alterations of the selected 12 -genes in OC using cBioPortal database. Among the 373 patients, 368 patients have mutations. The results showed that all queried genes were altered in 137 of 368 cases (37.23%). The 12-gene signature panel was confirmed as an independent prognostic indicator (P =2.29E-18, HR = 1.699, 95% CI = 1.508-1.913).

Conclusion: We established an effective m6A-related gene signature panel consisted of 12 -genes, which can predict the outcome of patients with OC. The high risk score indicates unfavorable survival. Our study provided novel insights into the relationship between m6A and OC. This gene signature panel will be helpful in identifying poor prognostic patients with OC and could be a promising prognostic indicator in clinical practice.
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http://dx.doi.org/10.2174/1574892816666210615164645DOI Listing
June 2021

PSP net-based automatic segmentation network model for prostate magnetic resonance imaging.

Comput Methods Programs Biomed 2021 May 29;207:106211. Epub 2021 May 29.

Department of Urology, the Fifth Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong 11100, China.

Purpose: Prostate cancer is a common cancer. To improve the accuracy of early diagnosis, we propose a prostate Magnetic Resonance Imaging (MRI) segmentation model based on Pyramid Scene Parsing Network (PSP Net).

Method: A total of 270 prostate MRI images were collected, and the data set was divided. Contrast limited adaptive histogram equalization (CLAHE) was enhanced in this study. We use the prostate MRI segmentation model based on PSP net, and use segmentation accuracy, under segmentation rate, over segmentation rate and receiver operating characteristic (ROC) curve evaluation index to compare the segmentation effect based on FCN and U-Net.

Results: PSP net has the highest segmentation accuracy of 0.9865, over segmentation rate of 0.0023, under segmentation rate of 0.1111, which is less than FCN and U-Net. The ROC curve of PSP net is closest to the upper left corner, AUC is 0.9427, larger than FCN and U-Net.

Conclusion: This paper proves through a large number of experimental results that the prostate MRI automatic segmentation network model based on PSP Net is able to improve the accuracy of segmentation, relieve the workload of doctors, and is worthy of further clinical promotion.
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http://dx.doi.org/10.1016/j.cmpb.2021.106211DOI Listing
May 2021

Heavy metals accumulation and risk assessment in a soil-maize (Zea mays L.) system around a zinc-smelting area in southwest China.

Environ Geochem Health 2021 Jun 16. Epub 2021 Jun 16.

College of Resources and Environmental Engineering, Guizhou University, Guiyang, 550025, China.

Smelting of nonferrous metals causes significant concerns because of its emissions of heavy metals (HMs) into surface soil, and its potential threat to human health through the food chain. To investigate the HMs concentrations in a soil-maize system, a total of 41 paired soil-maize samples were collected from a typical indigenous zinc-smelting area of northwestern Guizhou Province, China. Results showed that the concentrations of the targeted HMs in the soil were significantly higher than their corresponding background values of Guizhou Province. Results obtained of the geo-accumulation index (I) and the potential ecological risk factor of an individual metal (E ) revealed that Cd and Pb were identified as the top-priority control HMs in the study area. The mean concentrations in maize grain decreased in the order of Zn > Pb > Cu > Cd. Bio-accumulation factor (BAF) indicated a strong ability for Cd to be accumulated in the maize root. Translocation factor (TF) and Fourier transform infrared (FTIR) spectroscopy revealed that the maize root played an important role in reducing the mobilization of HMs to stem, except for Zn. Kriging interpolation results illustrated that the spatial distribution patterns of HMs in the maize grain were generally similar to those in sampled soil, and the higher concentrations for the investigated HMs were partially overlapping between soil and maize grain. The average hazard quotient (HQ) of the investigated HMs for both children and adults were all lower than the threshold value (HQ = 1). The total hazard index (HI) was 5.51E-01 and 4.24E-01 for the two population groups, respectively, implying no potential non-carcinogenic risk for local maize-consumers. Sensitivity analysis demonstrated that the oral ingestion rate (IngR) of grain was the predominated contribution to the output of the risk assessment.
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http://dx.doi.org/10.1007/s10653-021-01003-zDOI Listing
June 2021

Hydrogen-Binding-Initiated Activation of O-H Bonds on a Nitrogen-Doped Surface for the Catalytic Oxidation of Biomass Hydroxyl Compounds.

Angew Chem Int Ed Engl 2021 Jun 14. Epub 2021 Jun 14.

State Key Laboratory of Catalysis, Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, P. R. China.

Hydrogen binding of molecules on solid surfaces is an attractive interaction that can be used as the driving force for bond activation, material-directed assembly, protein protection, etc. However, the lack of a quantitative characterization method for hydrogen bonds (HBs) on surfaces seriously limits its application. We measured the standard Gibbs free energy change (ΔG ) of on-surface HBs using NMR. The HB-accepting ability of the surface was investigated by comparing ΔG values employing the model biomass platform 5-hydroxymethylfurfural on a series of Co-N-C-n catalysts with adjustable electron-rich nitrogen-doped contents. Decreasing ΔG improves the HB-accepting ability of the nitrogen-doped surface and promotes the selectively initiated activation of O-H bonds in the oxidation of 5-hydroxymethylfurfural. As a result, the reaction kinetics is accelerated. In addition to the excellent catalytic performance, the turnover frequency (TOF) for this oxidation is much higher than for reported non-noble-metal catalysts.
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http://dx.doi.org/10.1002/anie.202103604DOI Listing
June 2021

An Intelligent Graphene-Based Biosensing Device for Cytokine Storm Syndrome Biomarkers Detection in Human Biofluids.

Small 2021 Jul 10;17(29):e2101508. Epub 2021 Jun 10.

School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150001, China.

Abnormal elevated levels of cytokines such as interferon (IFN), interleukin (IL), and tumor necrosis factor (TNF), are considered as one of the prognosis biomarkers for indicating the progression to severe or critical COVID-19. Hence, it is of great significance to develop devices for monitoring their levels in COVID-19 patients, and thus enabling detecting COVID-19 patients that are worsening and to treat them before they become critically ill. Here, an intelligent aptameric dual channel graphene-TWEEN 80 field effect transistor (DGTFET) biosensing device for on-site detection of IFN-γ, TNF-α, and IL-6 within 7 min with limits of detection (LODs) of 476 × 10 , 608 × 10 , or 611 × 10 m respectively in biofluids is presented. Using the customized Android App together with this intelligent device, asymptomatic or mild COVID-19 patients can have a preliminary self-detection of cytokines and get a warning reminder while the condition starts to deteriorate. Also, the device can be fabricated on flexible substrates toward wearable applications for moderate or even critical COVID-19 cases for consistently monitoring cytokines under different deformations. Hence, the intelligent aptameric DGTFET biosensing device is promising to be used for point-of-care applications for monitoring conditions of COVID-19 patients who are in different situations.
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http://dx.doi.org/10.1002/smll.202101508DOI Listing
July 2021

Enhancement of the nutritional value of fermented corn stover as ruminant feed using the fungi Pleurotus spp.

Sci Rep 2021 Jun 7;11(1):11961. Epub 2021 Jun 7.

College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, China.

Four Pleurotus spp. fungi (P. diamor, P. eryngii, P. sajor-caju, P. citrinopileatus) were compared for their potential to improve nutritional value of corn stover as ruminant feed. Corn stover was inoculated with the fungi under solid-state conditions and their results showed that P. sajor-caju and P. eryngii were better than the other two species of Pleurotus with respect to decreasing the acid detergent lignin (ADL) (8.99 vs 9.88 vs 10.16 vs 10.46). In contrast, P. eryngii had lower ability to degrade cellulose (13.38%). Corn stover treated with P. citrinopileatus had the highest crude protein (CP) content (7.65%), whereas treatment with P. sajor-caju resulted in the highest increase in essential amino acids (55.11%). Although fungal pre-treatment of lignocellulosic biomass does not always result in high-quality feed, overall, P. eryngii and P. sajor-caju improved the nutritive value of corn stover as a ruminant feed.
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http://dx.doi.org/10.1038/s41598-021-90236-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185111PMC
June 2021

Nanobodies from camelid mice and llamas neutralize SARS-CoV-2 variants.

Nature 2021 07 7;595(7866):278-282. Epub 2021 Jun 7.

Lymphocyte Nuclear Biology, NIAMS, NIH, Bethesda, MD, USA.

Since the start of the COVID-19 pandemic, SARS-CoV-2 has caused millions of deaths worldwide. Although a number of vaccines have been deployed, the continual evolution of the receptor-binding domain (RBD) of the virus has challenged their efficacy. In particular, the emerging variants B.1.1.7, B.1.351 and P.1 (first detected in the UK, South Africa and Brazil, respectively) have compromised the efficacy of sera from patients who have recovered from COVID-19 and immunotherapies that have received emergency use authorization. One potential alternative to avert viral escape is the use of camelid VHHs (variable heavy chain domains of heavy chain antibody (also known as nanobodies)), which can recognize epitopes that are often inaccessible to conventional antibodies. Here, we isolate anti-RBD nanobodies from llamas and from mice that we engineered to produce VHHs cloned from alpacas, dromedaries and Bactrian camels. We identified two groups of highly neutralizing nanobodies. Group 1 circumvents antigenic drift by recognizing an RBD region that is highly conserved in coronaviruses but rarely targeted by human antibodies. Group 2 is almost exclusively focused to the RBD-ACE2 interface and does not neutralize SARS-CoV-2 variants that carry E484K or N501Y substitutions. However, nanobodies in group 2 retain full neutralization activity against these variants when expressed as homotrimers, and-to our knowledge-rival the most potent antibodies against SARS-CoV-2 that have been produced to date. These findings suggest that multivalent nanobodies overcome SARS-CoV-2 mutations through two separate mechanisms: enhanced avidity for the ACE2-binding domain and recognition of conserved epitopes that are largely inaccessible to human antibodies. Therefore, although new SARS-CoV-2 mutants will continue to emerge, nanobodies represent promising tools to prevent COVID-19 mortality when vaccines are compromised.
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http://dx.doi.org/10.1038/s41586-021-03676-zDOI Listing
July 2021

Neoantigen Specific T Cells Derived From T Cell-Derived Induced Pluripotent Stem Cells for the Treatment of Hepatocellular Carcinoma: Potential and Challenges.

Front Immunol 2021 13;12:690565. Epub 2021 May 13.

The First School of Clinical Medicine, Lanzhou University, Lanzhou, China.

Immunotherapy has become an indispensable part of the comprehensive treatment of hepatocellular carcinoma (HCC). Immunotherapy has proven effective in patients with early HCC, advanced HCC, or HCC recurrence after liver transplantation. Clinically, the most commonly used immunotherapy is immune checkpoint inhibition using monoclonal antibodies, such as CTLA-4 and PD-1. However, it cannot fundamentally solve the problems of a weakened immune system and inactivation of immune cells involved in killing tumor cells. T cells can express tumor antigen-recognizing T cell receptors (TCRs) or chimeric antigen receptors (CARs) on the cell surface through gene editing to improve the specificity and responsiveness of immune cells. According to previous studies, TCR-T cell therapy is significantly better than CAR-T cell therapy in the treatment of solid tumors and is one of the most promising immune cell therapies for solid tumors so far. However, its application in the treatment of HCC is still being researched. Technological advancements in induction and redifferentiation of induced pluripotent stem cells (iPSCs) allow us to use T cells to induce T cell-derived iPSCs (T-iPSCs) and then differentiate them into TCR-T cells. This has allowed a convenient strategy to study HCC models and explore optimal treatment strategies. This review gives an overview of the major advances in the development of protocols to generate neoantigen-specific TCR-T cells from T-iPSCs. We will also discuss their potential and challenges in the treatment of HCC.
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http://dx.doi.org/10.3389/fimmu.2021.690565DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155510PMC
May 2021

Risk Factors and Survival of Patients With Liver Metastases at Initial Metastatic Breast Cancer Diagnosis in Han Population.

Front Oncol 2021 11;11:670723. Epub 2021 May 11.

Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

The risk factors for morbidity and mortality in patients with breast cancer liver metastases (BCLM) upon initial metastatic breast cancer (MBC) diagnosis have not been adequately identified in Han population. Data of 3,161 female patients who were initially diagnosed with MBC from December 1991 to September 2019 and treated in the China National Cancer Center were extracted and a total of 2,263 MBC patients were included in our study, among which 550 patients had liver metastases. Multivariable logistic regression was performed to identify risk factors for the presence of liver metastases at initial MBC diagnosis. Univariable and multivariable Cox proportional hazards regression analyses were conducted to determine prognostic factors for the survival of BCLM patients. Patients with hormone receptor (HR)-negative, human epidermal growth factor receptor 2 (HER2)-positive (35.0% of the entire population) subtype had the highest incidence of liver metastases. stage IV breast cancer, HR-/HER2+ and HR+/HER2+ subtypes were associated with higher odds of liver metastases and patients with lung metastases had lower risk of liver metastases at initial MBC diagnosis. The median overall survival of BCLM patients was 31.4 months and BCLM patients with HR+/HER2- subtype had the longest survival of 38.2 months. Older age, worse performance status, later stage of initial breast cancer, triple-negative subtype and lung metastases were significantly associated with a poorer prognosis in BCLM patients. Our study offers insights into the incidence and prognosis of BCLM patients at initial MBC diagnosis in Han population.
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http://dx.doi.org/10.3389/fonc.2021.670723DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147724PMC
May 2021

Phase II study of apatinib in combination with oral vinorelbine in heavily pretreated HER2-negative metastatic breast cancer and clinical implications of monitoring ctDNA.

Cancer Biol Med 2021 May 26. Epub 2021 May 26.

Unit of Medical Oncology, Fondazione Policlinico Universitario A. Gemelli IRCCS, Roma 00176, Italy.

Objective: Apatinib is an oral TKI targeting VEGFR-2. Single-agent apatinib treatment has been shown to produce an objective response in patients with pretreated mBC. Oral vinorelbine also holds promise as a treatment of choice in patients with mBC. This study aimed to investigate the efficacy and safety of the oral vinorelbine-apatinib combination in patients with pretreated mBC. In addition, we detected gene variants in ctDNA to explore the therapeutic implications.

Methods: This study enrolled patients with HER2-negative mBC who were pretreated with anthracycline/taxanes. Patients were treated with apatinib at 500 mg/425 mg daily plus oral vinorelbine 60 mg/m on days 1, 8, and 15 of every cycle (3 weeks). The primary endpoint was PFS. The secondary endpoints were ORR, CBR, OS, and safety. Patients eligible for ctDNA detection were evaluated before and during treatment.

Results: Forty patients were enrolled. The median PFS was 5.2 months (95% CI, 3.4-7.0 months), and the median OS was 17.4 months (95% CI, 8.0-27.0 months). The ORR was 17.1% (6/35), and the CBR was 45.7% (16/35). The most common AEs included gastrointestinal reaction, myelosuppression, and hypertension. In 20 patients, ctDNA was detected at baseline and during treatment. A significant difference was found in PFS for undetected detected baseline ctDNA (13.9 months 3.6 months, = 0.018).

Conclusions: All-oral therapy with apatinib plus vinorelbine displayed objective efficacy in patients with heavily pretreated HER2-negative mBC, with acceptable and manageable toxicity profiles. Patients with no gene variant detected and lower variant allele frequencies in ctDNA at baseline showed longer PFS.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0418DOI Listing
May 2021

[Outcome of modified Kidner procedure with tendoscopy for the treatment of painful accessory navicular].

Zhongguo Gu Shang 2021 May;34(5):448-51

Department of Orthopaedics, General Hospital of PLA, Beijing 100853, China.

Objective: To evaluate clinical efficacy of modified kidner procedure with tendoscopy in treating painful accessory navicular.

Methods: From February 2014 to April 2019, 19 patients with painful accessory navicular were admitted, including 13 males and 6 females with a mean age of 26 years old (ranged from 14 to 58 years old), all of which were unilateral symptoms. The courses of disease ranged from 6 to 60 months. All patients received excision of accessory navicular and reconstruction of posterior tibial tendon insertion on navicular with anchor, and the tendoscopy were used to examin the posterior tibial tendon. American Orthopedic Foot and Ankle Society(AOFAS) midfoot score and visual analogue scale(VAS) were used to evaluate efficacy before operation and at the latest follow-up.

Results: All the patientswere followed up, and the duration ranged from 12 to 73 months, with an average of (35.0±20.9) months. VAS score was 0.20±0.41 at the latest follow-up, showing significant difference when compared with preoperative score of 6.33±1.95(=11.50, <0.01). AOFAS score was 95.53±5.79 at the latest follow-up, showing significant difference when compared with preoperative score of 60.27±21.51 (=5.71, <0.01).

Conclusion: The modified kidner procedure with tendoscopy is a good choice for the treatment of painful accessory navicular, which could obviously relieve foot pain, improve foot function, and has certain clinical efficacy.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2021.05.010DOI Listing
May 2021

Multimodally profiling memory T cells from a tuberculosis cohort identifies cell state associations with demographics, environment and disease.

Nat Immunol 2021 06 24;22(6):781-793. Epub 2021 May 24.

Center for Data Sciences, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.

Multimodal T cell profiling can enable more precise characterization of elusive cell states underlying disease. Here, we integrated single-cell RNA and surface protein data from 500,089 memory T cells to define 31 cell states from 259 individuals in a Peruvian tuberculosis (TB) progression cohort. At immune steady state >4 years after infection and disease resolution, we found that, after accounting for significant effects of age, sex, season and genetic ancestry on T cell composition, a polyfunctional type 17 helper T (T17) cell-like effector state was reduced in abundance and function in individuals who previously progressed from Mycobacterium tuberculosis (M.tb) infection to active TB disease. These cells are capable of responding to M.tb peptides. Deconvoluting this state-uniquely identifiable with multimodal analysis-from public data demonstrated that its depletion may precede and persist beyond active disease. Our study demonstrates the power of integrative multimodal single-cell profiling to define cell states relevant to disease and other traits.
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http://dx.doi.org/10.1038/s41590-021-00933-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162307PMC
June 2021

Lymphadenectomy Around Inferior Mesenteric Artery in Low-Tie vs High-Tie Laparoscopic Anterior Resection: Short- and Long-Term Outcome of a Cohort of 614 Rectal Cancers.

Cancer Manag Res 2021 14;13:3963-3971. Epub 2021 May 14.

Department of Gastrointestinal Surgery, Renji Hospital, Jiao Tong University School of Medicine, Shanghai, 200127, People's Republic of China.

Background: Preservation of the left colic artery in low-tie (LT) of inferior mesenteric artery remains controversial compared to high-tie (HT) in the colon and rectal cancers, for lymph node dissection, anastomotic leakage, and oncological outcome. This cohort study aims to analyze short- and long-term outcomes of laparoscopic anterior resections in LT vs HT for rectal cancers.

Methods: We analyzed a cohort of laparoscopic AR for RC from 2013 to 2016 at Renji Hospital, Shanghai, China. Short- and long-term outcome in LT vs HT group were compared for clinico-demographic characteristics, operative-time, lymph node dissection, short-term 30-day outcome, and long-term 3- and 5-year overall survival as well as disease-free survival. The x, -test, and logistic regressions analysis were used and p<0.05 was considered significant.

Results: The cohort consisted of 614 laparoscopic AR with LT (236) and HT (378). The clinicodemographic characteristics were comparable among the groups. The surgery took longer in LT. The yield of LND was similar. Leakage occurred in 12.21% (n=75). Leakage was fewer in LT than HT, 8.89% vs 14.28%, p=0.047. The postoperative severe complications were higher in HT. The 30-day mortality was nil. The long-term 3- and 5-year overall survival and disease-free survival were similar in LT and HT.

Conclusion: The LT with preservation of left colic artery had similar lymph node yield, but lower leakage and complications than HT in laparoscopic anterior resections for rectal cancers. The long-term 3- and 5-year overall and disease-free survival were similar in the two groups.
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http://dx.doi.org/10.2147/CMAR.S282986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131009PMC
May 2021

Cystoscopy to remove an intrauterine contraceptive device embedded in the urinary bladder wall: a case report and literature review.

J Int Med Res 2021 May;49(5):3000605211015032

Division of Urology, Department of Minimally Invasive Surgery, West China No. 4 Hospital, Sichuan University, Chengdu, 610041 PR China.

Migration and embedding of an intrauterine contraceptive device (IUCD) in the urinary bladder wall is rare. We present such a case of a 30-year-old woman with complaints of persistent lower urinary tract symptoms and a history of IUCD placement 8 years earlier. The IUCD was successfully removed with cystoscopy alone. The patient recovered well and had her second baby after the surgery without complaints of new urinary symptoms.
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http://dx.doi.org/10.1177/03000605211015032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8135220PMC
May 2021

Estimating heritability and its enrichment in tissue-specific gene sets in admixed populations.

Hum Mol Genet 2021 May 13. Epub 2021 May 13.

Center for Data Sciences, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

It is important to study the genetics of complex traits in diverse populations. Here, we introduce covariate-adjusted LD score regression (cov-LDSC), a method to estimate SNP-heritability (${\boldsymbol{h}}_{\boldsymbol{g}}^{\mathbf{2}}\Big)$ and its enrichment in homogenous and admixed populations with summary statistics and in-sample LD estimates. In-sample LD can be estimated from a subset of the GWAS samples, allowing our method to be applied efficiently to very large cohorts. In simulations, we show that unadjusted LDSC underestimates ${\boldsymbol{h}}_{\boldsymbol{g}}^{\mathbf{2}}$ by 10%-60% in admixed populations; in contrast, cov-LDSC is robustly accurate. We apply cov-LDSC to genotyping data from 8124 individuals, mostly of admixed ancestry, from the Slim Initiative in Genomic Medicine for the Americas (SIGMA) study, and to approximately 161 000 Latino-ancestry individuals, 47 000 African American-ancestry individuals, and 135 000 European-ancestry individuals, as classified by 23andMe. We estimate ${\boldsymbol{h}}_{\boldsymbol{g}}^{\mathbf{2}}$ and detect heritability enrichment in three quantitative and five dichotomous phenotypes, making this, to our knowledge, the most comprehensive heritability-based analysis of admixed individuals to date. Most traits have high concordance of ${\boldsymbol{h}}_{\boldsymbol{g}}^{\mathbf{2}}$ and consistent tissue-specific heritability enrichment among different populations. However, for age at menarche, we observe population-specific heritability estimates of ${\boldsymbol{h}}_{\boldsymbol{g}}^{\mathbf{2}}$. We observe consistent patterns of tissue-specific heritability enrichment across populations; for example, in the limbic system for BMI, the per-standardized-annotation effect size 𝛕* is 0.16 ± 0.04, 0.28 ± 0.11 and 0.18 ± 0.03 in the Latino-, African American- and European-ancestry populations, respectively. Our approach is a powerful way to analyze genetic data for complex traits from admixed populations.
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http://dx.doi.org/10.1093/hmg/ddab130DOI Listing
May 2021

Hsa_circ_0091581 promotes glioma progression by regulating RMI1 via sponging miR-1243-5p.

J Cancer 2021 2;12(11):3249-3256. Epub 2021 Apr 2.

Department of Neurosurgery, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.

Glioma is a pervasive malignancy and the main cause of cancer-related deaths worldwide. Circular RNA is an important subject of cancer research, and its role and function in glioma are poorly understood. This study demonstrated that hsa_circ_0091581 is upregulated in glioma tissues and cells. The results of the CCK-8, EdU, and transwell assays indicated that hsa_circ_0091581 promotes proliferation, migration, and invasion of glioma cells. The results of the luciferase reporter and RNA immunoprecipitation assays indicated that the mechanism of the effects of hsa_circ_0091581 on glioma cells involves sponging miR-1243-5p to regulate RMI1. The results of the rescue experiments indicated that hsa_circ_0091581 regulates proliferation, migration, and invasion of glioma cells by targeting RMI1 in a miR-1243-5p dependent manner. The results of the nude mice xenograft assays showed that knockdown of hsa_circ_0091581 inhibits glioma growth . Thus, our study determined the role of hsa_circ_0091581/miR-1243-5p/RMI1 in glioma and suggests that this axis may be a novel therapeutic target in glioma.
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http://dx.doi.org/10.7150/jca.55558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100801PMC
April 2021

M2 Macrophages Promote PDGFRβ Pericytes Migration After Spinal Cord Injury in Mice PDGFB/PDGFRβ Pathway.

Front Pharmacol 2021 15;12:670813. Epub 2021 Apr 15.

Department of Orthopaedics, The Second Hospital of Anhui Medical University, Hefei, China.

Platelet derived growth factor receptor β positive (PDGFRβ) pericytes form fibrotic scar, which prevents axonal regeneration after spinal cord injury (SCI). However, the mechanism by which PDGFRβ pericytes migrate to the injury core is unclear. Here, we investigated the effect and mechanism of macrophages polarization on PDGFRβ pericytes migration after SCI. Macrophages were closely related to the spatiotemporal distribution of PDGFRβ pericytes in the injury core at 3, 7, and 14 days postinjury (dpi). Macrophages appeared M2 polarization at 3 and 7 dpi while M1 polarization at 14 dpi. The expression of platelet derived growth factor B (PDGFB) was significantly increased after SCI and after macrophages M2 polarization. The promoting effect of exogenous PDGFB and M2 macrophages conditioned medium on PDGFRβ pericytes migration could be blocked by SU16f, a PDGFRβ specific inhibitor. These findings indicate that M2 macrophages can secrete PDGFB acting on PDGFRβ to promote PDGFRβ pericytes migration, which can be blocked by a PDGFRβ specific inhibitor SU16f. The PDGFB/PDGFRβ pathway is a promising new target for the treatment of SCI.
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http://dx.doi.org/10.3389/fphar.2021.670813DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082415PMC
April 2021

Bifunctional Ag-Decorated CeO Nanorods Catalysts for Promoted Photodegradation of Methyl Orange and Photocatalytic Hydrogen Evolution.

Nanomaterials (Basel) 2021 Apr 24;11(5). Epub 2021 Apr 24.

Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China.

The photodegradation of organic pollutants and photocatalytic hydrogen generation from water by semiconductor catalysts are regarded as the of the most promising strategies to resolve the crisis of global environmental issues. Herein, we successfully designed and prepared a series of silver-decorated CeO(Ag/CeO) photocatalysts with different morphologies by a facile hydrothermal route. The physical properties, charge transfer behavior and photocatalytic performances (degradation and hydrogen evolution) over diverse catalysts with nanocubes, nanoparticles and nanorods shapes were comprehensively studied. It was found that the Ag-decorated CeO nanorods (Ag/R-CeO) demonstrate the best activity for both photocatalytic methyl orange (MO) degradation and photocatalytic H production reaction with attractive stability during cycling tests, suggesting its desirable practical potential. The superior performance of Ag/R-CeO can be ascribed to (1) the facilitated light absorption due to enriched surface oxygen vacancies (OVs) and plasmonic Ag nanoparticles on nanorods, (2) the facilitated photo-excited charge carrier (e-h) separation efficiency on a metal/oxide hybrid structure and (3) the promoted formation of active reaction intermediates on surface-enriched Ag and oxygen vacancies reactive sites on Ag/CeO nanorods. This study provides a valuable discovery of the utilization of abundant solar energy for diverse catalytic processes.
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http://dx.doi.org/10.3390/nano11051104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145711PMC
April 2021

Zinc salt-catalyzed reduction of α-aryl imino esters, diketones and phenylacetylenes with water as hydrogen source.

Org Biomol Chem 2021 Apr;19(16):3601-3610

Key Laboratory of Chemistry in Ethnic Medicinal Resources (Yunnan Minzu University), State Ethnic Affairs Com-mission & Ministry of Education, Kunming, 650500, China. and Chongqing Key Laboratory of traditional Chinese medicine health, Chongqing Accademy of Chinese Materia Medica, Chongqing, China.

The zinc salt-catalyzed reduction of α-aryl imino esters, diketones and phenylacetylenes with water as hydrogen source and zinc as reductant was successfully conducted. The presented method provides a low-cost, environmentally friendly and practical preparation of α-aryl amino esters, α-hydroxyketones and phenylethylenes. By using D2O as deuterium source, the corresponding products were obtained in high efficiency with excellent deuterium incorporation rate, which gives a cheap and safe tool for access to valuable deuterium-labelled compounds.
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http://dx.doi.org/10.1039/d1ob00155hDOI Listing
April 2021

Safety and efficacy study of oral metronomic vinorelbine combined with trastuzumab (mNH) in HER2-positive metastatic breast cancer: a phase II trial.

Breast Cancer Res Treat 2021 Jul 25;188(2):441-447. Epub 2021 Apr 25.

Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, No.17, Panjiayuan Nanli, Chaoyang District, Beijing, 100021, People's Republic of China.

Purpose: We conducted a single-arm prospective phase II trial to evaluate the efficacy and safety of oral metronomic vinorelbine combined with trastuzumab (mNH) in human epidermal growth factor receptor 2-positive (HER2-positive) metastatic breast cancer (MBC) patients.

Methods: HER2-positive MBC patients received oral vinorelbine 40 mg thrice a week and trastuzumab (loading dose of 8 mg/kg, followed by 6 mg/kg every 3 weeks) until disease progression, unacceptable toxicity, or consent withdrawal. The primary endpoints were objective response rate (ORR), clinical benefit rate (CBR; CR + PR + SD for ≥ 24 weeks). The secondary endpoints were progression-free survival (PFS), tolerability, and overall survival (OS).

Results: Twenty patients with HER2-positive MBC were enrolled, with a median of 1 prior chemotherapy regimens for MBC. Median age was 61.5 years (95% Confidence Interval (CI) 48.6-63.1). Visceral involvements presented in 14 patients (70.0%). ORR was 20.0%, and CBR was 75% with 4 PR (20.0%) and 11 SD (55.0%). The median PFS (mPFS) and median OS (mOS) were 7.4 months (95% CI 3.2-11.5) and 45.8 months (95%CI: not reached), respectively. The mPFS was 17.7 months (95%CI not reached) and 5.8 months (95%CI 5.6-5.9) in mNH as first-line and ≥ second-line therapy (log rank p = 0.03), respectively. The most common grade 1 adverse events (AEs) included nausea (15%), leukopenia (15%), ALT/AST elevation (15%), diarrhea (10%), and peripheral neuropathy (10%). Grade 2 adverse events included leukopenia (5%) and neutropenia (10%). No grade 3/4 AEs were observed.

Conclusions: Oral metronomic vinorelbine combined with trastuzumab is a well-tolerated and effective anti-tumor regimen for HER2-positive MBC.
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http://dx.doi.org/10.1007/s10549-021-06216-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070982PMC
July 2021

Outer membrane vesicles mediated horizontal transfer of an aerobic denitrification gene between Escherichia coli.

Biodegradation 2021 08 22;32(4):435-448. Epub 2021 Apr 22.

Department of Environmental Engineering, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.

Bacterial genetic material can be horizontally transferred between microorganisms via outer membrane vesicles (OMVs) released by bacteria. Up to now, the application of vesicle-mediated horizontal transfer of "degrading genes" in environmental remediation has not been reported. In this study, the nirS gene from an aerobic denitrification bacterium, Pseudomonas stutzeri, was enclosed in a pET28a plasmid, transformed into Escherichia coli (E. coli) DH5α and expressed in E. coli BL21. The E. coli DH5α released OMVs containing the recombination plasmid pET28a-nirS-EGFP. When compared with the free pET28a-nirS-EGFP plasmid's inability to transform, nirS in OMVs could be transferred into E. coli BL21 with the transformation frequency of 2.76 × 10 CFU/g when the dosage of OMVs was 200 µg under natural conditions, and nirS could express successfully in recipient bacteria. Furthermore, the recipient bacteria that received OMVs containing pET28a-nirS-EGFP could produce 18.16 U/mL activity of nitrite reductase.
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http://dx.doi.org/10.1007/s10532-021-09945-yDOI Listing
August 2021

Flipped Quick-Response Code Enables Reliable Blood Grouping.

ACS Nano 2021 04 19;15(4):7649-7658. Epub 2021 Apr 19.

Center of Smart Laboratory and Molecular Medicine, School of Medicine, Chongqing University, Chongqing 400044, People's Republic of China.

Accurate and rapid blood typing plays a vital role in a variety of biomedical and forensic scenarios, but recognizing weak agglutination remains challenging. Herein, we demonstrated a flipping identification with a prompt error-discrimination (FLIPPED) platform for automatic blood group readouts. Bromocresol green dye was exploited as a characteristic chromatography indicator for the differentiation of plasma from whole blood by presenting a teal color against a brown color. After integrating these color changes into a quick-response (QR) code, prompt typing of ABO and Rhesus groups was automatically achieved and data could be uploaded wirelessly within 30 s using a commercially available smartphone to facilitate blood cross-matching. We further designed a color correction model and algorithm to remove potential errors from scanning angles and ambient light intensities, by which weak agglutination could be accurately recognized. With comparable accuracy and repeatability to classical column assay in grouping 450 blood samples, the proposed approach further demonstrates to be a versatile sample-to-result platform for clinical diagnostics, food safety, and environmental monitoring.
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http://dx.doi.org/10.1021/acsnano.1c01215DOI Listing
April 2021

Trace the profile and function of circular RNAs in Sertoli cell only syndrome.

Genomics 2021 Jul 15;113(4):1845-1854. Epub 2021 Apr 15.

NHC Key Laboratory of Human Stem Cell and Reproductive Engineering, Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, Hunan, China; Clinical Research Center for Reproduction and Genetics in Hunan Province, Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, Hunan, China. Electronic address:

Studies increasingly show the involvement of circular RNAs (circRNAs) in several diseases. This study aims to explore the circRNA expression pattern in the testicular tissues of patients with Sertoli only cell syndrome (SCOS) and their potential functions. High throughput circRNA microarray analysis indicated that 399 circRNAs were upregulated and 1195 were down-regulated (fold change >2, P < 0.05) in SCOS relative to obstructive azoospermia (OA). The hsa_circRNA_101222, hsa_circRNA_001387, hsa_circRNA_001153, hsa_circRNA_101373 and hsa_circRNA_103864 were validated by qRT-PCR. Furthermore, the hosting genes of the differentially expressed circRNAs (DEcircRNAs) were enriched in biological processes related to cell cycle and intercellular communication. Also, the overlapping genes between the hosting genes of SCOS-related DEcircRNAs and those highly expressed in Sertoli cells of non-obstructive azoospermia (NOA) were enriched in immune cell development and cell communication. Taken together, aberrantly expressed circRNAs likely mediate SCOS development by regulating the function of Sertoli cells and the spermatogenic microenvironment.
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http://dx.doi.org/10.1016/j.ygeno.2021.04.022DOI Listing
July 2021