Publications by authors named "Yang Jiang"

757 Publications

Purvalanol A induces apoptosis and reverses cisplatin resistance in ovarian cancer.

Anticancer Drugs 2022 Aug 9. Epub 2022 Aug 9.

Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan, Hubei Province 430060, P.R. China.

Cisplatin (DDP) resistance limits therapeutic efficacy in patients diagnosed with ovarian cancer. Purvalanol A (Pur) is a novel cyclin-dependent kinase (CDK) inhibitor that has been demonstrated to induce apoptosis in various cancer cells. The present study investigated the effect of the combination treatment of Pur and DDP, and the potential anticancer mechanisms in epithelial ovarian cancer (EOC) cells in vitro and in vivo. We found that Pur enhanced the anti-tumor efficacy of cisplatin in EOC cells. The combination of Pur and DDP had more significant effects on apoptosis induction in EOC cells compared with the individual-treatment groups and the control group. We further demonstrated that the combination of Pur and DDP may trigger apoptosis and autophagy in EOC cells by inducing reactive oxygen species (ROS). And the ROS/Akt/mammalian target of rapamycin signaling pathway as a potential mechanism for the initiation of autophagy induced by combination therapy. Similar results were observed in vivo. These results demonstrated that Pur sensitized the response of EOC cells to cisplatin in vitro and in vivo, reversing the resistance to cisplatin in ovarian cancer.
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http://dx.doi.org/10.1097/CAD.0000000000001339DOI Listing
August 2022

Study on screening and evaluation methods of cosmetics for people with facial sensitive skin.

Medicine (Baltimore) 2022 Aug;101(31):e29975

Department of Dermatology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, P.R. China.

Objective: The purpose of this study is to screen and evaluate cosmetic products for sensitive skin on the face.

Methods: Thirty-five subjects with positive lactic acid sting test (LAST) were recruited from the staff of our hospital from November 2019 to February 2020. First, the human skin enclosed patch test of cosmetic gel (abbreviated as gel) was performed, and then the tested products were continuously applied for 4 weeks to complete the long-term efficacy test. Subjects' sensation of application, pruritus, tingling and burning were assessed on a 0 to 9 scale prior to, 14, and 28 days after topical application. Moreover, the transepidermal water loss rate (TEWL), stratum corneum (SC) hydration, melanin index (MI), erythema index (EI) and dendritic cells and inflammatory cell infiltration were noninvasively detected by the tester. LAST were performed before applying, 14 and 28 days after application, and then the test results were compared.

Results: In this study, a total of 34 people participated in the test. The results of human skin enclosed patch test indicated that only 1 case of grade 1 reaction occurred among the tested subjects. The subjects felt good after applying the products, and the gel showed high degree of skin comfortable, no irritation and good tolerability. Subjective safety evaluation illustrated that the scores of pruritus, tingling and burning of the subjects decreased in D14 and D28 patient revisit, showing statistically significant differences (P < .05). When the gel was applied for 4 weeks, TEWL (8.42 ± 1.12) and EI (201.35 ± 13.51) were lower than the results before application (P < .05), and the SC hydration (65.36 ± 2.56) was higher than that before application (P < .05). There was no correlation between the SC hydration and TEWL (R = 0.092, P = .416). The results of skin CT indicated that the number of facial dendritic cells decreased in 17 subjects (accounting for 50%) in D28 patient revisit, and the degree of inflammatory cell infiltration decreased in 27 subjects (accounting for 80%). Compared with the baseline value, the LAST score and total sensory score decreased after application the product for 4 weeks, and the difference was statistically significant (the mean value of P < .05).

Conclusion: The subjective feeling of application and efficacy of cosmetics in people with sensitive skin could be successfully evaluated by the comprehensive application of human skin enclosed patch test, long-term trial test, subjective safety evaluation and objective efficacy evaluation. And it provides the basis to judge whether the cosmetic is consistent with the efficacy claim of sensitive skin.
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http://dx.doi.org/10.1097/MD.0000000000029975DOI Listing
August 2022

CircKPNB1 mediates a positive feedback loop and promotes the malignant phenotypes of GSCs via TNF-α/NF-κB signaling.

Cell Death Dis 2022 Aug 9;13(8):697. Epub 2022 Aug 9.

Department of Neurosurgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China.

Glioma stem cells (GSCs) are a special kind of cells in GBM showing tumor initiation, self-renewal, and multi-lineage differentiation abilities. Finding novel circRNAs related to GSCs is of great significance for the study of glioma. qPCR, western blotting, and immunohistochemistry were used to detect the expression levels of circKPNB1, SPI1, DGCR8, and TNF-α. The expression of these molecules in GSCs was regulated by lentiviral-based infection. RNA immunoprecipitation assay, RNA pull-down, dual-luciferase reporter, and chromatin immunoprecipitation assays were used to study the direct regulation mechanisms among these molecules. All the MTS, EDU, transwell, neurosphere formation assays, ELDA assays, and xenograft experiments were used to detect the malignant phenotype of GSCs. We found a novel circRNA circKPNB1 was overexpressed in GBM and associated with GBM patients' poor prognosis. CircKPNB1 overexpression can promote the cell viabilities, proliferation, invasion, neurospheres formation abilities, and stemness of GSCs. Mechanistically, circKPNB1 regulates the protein stability and nuclear translocation of SPI1. SPI1 promotes the malignant phenotype of GSCs via TNF-α mediated NF-κB signaling. SPI1 can also transcriptionally upregulate DGCR8 expression, and the latter can maintain the stability of circKPNB1 and forms a positive feedback loop among DGCR8, circKPNB1 and SPI1. Our study found circKPNB1 was a novel oncogene in GBM and of great significance in the diagnosis and prognosis prediction of GBM and maybe a novel target for molecular targeted therapy.
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http://dx.doi.org/10.1038/s41419-022-05149-1DOI Listing
August 2022

Enriched-bromine surface state for stable sky-blue spectrum perovskite QLEDs with an EQE of 14.6.

Adv Mater 2022 Jul 29:e2205092. Epub 2022 Jul 29.

School of Materials Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China.

Halogen vacancies are of great concern in blue-emitting perovskite quantum dot light-emitting diodes because they affect their efficiency and spectral shift. Here, the enriched-bromine surface state is realised using a facile strategy that employs a PbBr stock solution for anion exchange based on Cd-doped perovskite quantum dots. We find that the doped Cd ions are expected to reduce the formation energy of halogen vacancies filled by the external bromine ions, and the excess free bromine ions in solution are enriched in the surface by anchoring with halogen vacancies as sites, accompanied with the shedding of surface long-chain ligands during the anion exchange process, resulting in a Br-rich and "neat" surface. Moreover, the surface state exhibits good passivation of the surface defects of the controlled perovskite QDs and simultaneously increases the exciton binding energy, leading to excellent optical properties and stability. Finally, the sky-blue emitting perovskite QLEDs (490 nm) are conducted with a record external quantum efficiency of 14.6% and current efficiency of 19.9 cd/A. Meanwhile, the electroluminescence spectra exhibit great stability with negligible shifts under a constant operating voltage from 3 to 7 V. This strategy paves the way for improving the efficiency and stability of perovskite QLEDs. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202205092DOI Listing
July 2022

Reducing White Adipose Tissue Browning Using p38α MAPK Inhibitors Ameliorates Cancer-Associated Cachexia as Assessed by Magnetic Resonance Imaging.

Nutrients 2022 Jul 22;14(15). Epub 2022 Jul 22.

Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, China.

Background: Up to 80% of pancreatic cancer patients suffer from cachexia. White adipose tissue (WAT) browning caused by the tumorigenicity and progression aggravates the cancer-associated cachexia (CAC). Cancer-initiated changes in the protein-38 mitogen-activated protein kinases (p38 MAPK) pathway are likely involved in the development of CAC.

Methods: p38 MAPK inhibitors, VCP979 or SB203580, were used in the and models of pancreatic cancer cachexia. Expression of uncoupling protein 1 (UCP1) in the p38 MARK pathway and the properties and level of white adipocytes were analyzed and correlated to browning, followed by immunohistochemistry and Western blotting validations. Changes in the volume and fat fraction of WAT in animals were monitored by magnetic resonance imaging (MRI).

Results: The size of white adipocytes was increased after being treated with the p38 MAPK inhibitors, along with increase in the MRI-measured volume and fat fraction of WAT. Comparing two p38 MAPK inhibitors, the p38α subunit-specific inhibitor VCP979 had a better therapeutic effect than SB203580, which targets both p38α and β subunits.

Conclusions: Blockade of p38 MAPK reduced the WAT browning that contributes to CAC. Thus, p38 MARK inhibitors can potentially be used as a therapy for treating CAC. Non-invasive MRI can also be applied to assess the progression and treatment responses of CAC.
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http://dx.doi.org/10.3390/nu14153013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331061PMC
July 2022

Oscillator-Network-Based Ising Machine.

Micromachines (Basel) 2022 Jun 27;13(7). Epub 2022 Jun 27.

Faculty of Engineering, The University of Hong Kong, Hong Kong 999077, China.

With the slowdown of Moore's law, many emerging electronic devices and computing architectures have been proposed to sustain the performance advancement of computing. Among them, the Ising machine is a non-von-Neumann solver that has received wide attention in recent years. It is capable of solving intractable combinatorial optimization (CO) problems, which are difficult to be solve using conventional digital computers. In fact, many CO problems can be mapped to finding the corresponding ground states of Ising model. At present, Ising machine prototypes based on different physical principles, such as emerging memristive oscillators, have been demonstrated, among which the Ising Hamiltonian solver based on the coupled oscillator network simultaneously holds the advantages of room-temperature operation, compact footprint, low power consumption, and fast speed to solution. This paper comprehensively surveys the recent developments in this important field, including the types of oscillators, the implementation principle of the Ising model, and the solver's performance. Finally, methods to further improve the performance have also been suggested.
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http://dx.doi.org/10.3390/mi13071016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319543PMC
June 2022

Amine-Modified Chitosan Flocculant Synthesized via Single-Mode Microwave Method for Laundry Wastewater Treatment.

ACS Omega 2022 Jul 5;7(28):24522-24530. Epub 2022 Jul 5.

Liaoning Petrochemical University, Dandong Road, Wanghua District, Fushun City, Liaoning Province 113001, China.

In this study, an effective and environmentally friendly polyaminated cross-linked chitosan (M-PACTS) flocculant was successfully synthesized via circular focus single-mode microwave synthesizer irradiation. Epichlorohydrin and tetraethylenepentamine were used as the cross-linking agent and active cationic reagent, respectively. The same formation was used to prepare cationic lightly cross-linked chitosan (C-PACTS) via the conventional heating method. The flocculant was characterized using Fourier transform infrared spectroscopy, X-ray diffraction analysis, and scanning electron microscopy. The flocculation capability of C-PACTS and M-PACTS was compared using laundry wastewater as a model pollutant. The pH, PACTS dosage, temperature, stirring rate, stirring time, and setting time were systematically investigated. The experimental results showed that circular focus single-mode microwave synthesizer irradiation was a more efficient method to modify chitosan. M-PACTS exhibited a higher capacity for turbidity and chemical oxygen demand (COD) removal. Under optimal conditions, the removal rate values of M-PACTS were up to 96% (turbidity) and 78% (COD). The proposed PACTS is suitable for treatment of polluted wastewater in an eco-friendly manner without causing secondary pollution.
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http://dx.doi.org/10.1021/acsomega.2c02189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301697PMC
July 2022

UPF1/circRPPH1/ATF3 feedback loop promotes the malignant phenotype and stemness of GSCs.

Cell Death Dis 2022 Jul 23;13(7):645. Epub 2022 Jul 23.

Department of Neurosurgery, The First Hospital of China Medical University, Shenyang, China.

Glioblastoma multiforme (GBM) is the most lethal type of craniocerebral gliomas. Glioma stem cells (GSCs) are fundamental reasons for the malignancy and recurrence of GBM. Revealing the critical mechanism within GSCs' self-renewal ability is essential. Our study found a novel circular RNA (circRPPH1) that was up-regulated in GSCs and correlated with poor survival. The effect of circRPPH1 on the malignant phenotype and self-renewal of GSCs was detected in vitro and in vivo. Mechanistically, UPF1 can bind to circRPPH1 and maintain its stability. Therefore, more existing circRPPH1 can interact with transcription factor ATF3 to further transcribe UPF1 and Nestin expression. It formed a feedback loop to keep a stable stream for stemness biomarker Nestin to strengthen tumorigenesis of GSCs continually. Besides, ATF3 can activate the TGF-β signaling to drive GSCs for tumorigenesis. Knocking down the expression of circRPPH1 significantly inhibited the proliferation and clonogenicity of GSCs both in vitro and in vivo. The overexpression of circRPPH1 enhanced the self-renewal of GSCs. Our findings suggest that UPF1/circRPPH1/ATF3 maintains the potential self-renewal of GSCs through interacting with RNA-binding protein and activating the TGF-β signal pathway. Breaking the feedback loop against self-renewing GSCs may represent a novel therapeutic target in GBM treatment.
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http://dx.doi.org/10.1038/s41419-022-05102-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308777PMC
July 2022

Synergistic hydroxyl radical formation, system XC- inhibition and heat shock protein crosslinking tango in ferrotherapy: A prove-of-concept study of "sword and shield" theory.

Mater Today Bio 2022 Dec 7;16:100353. Epub 2022 Jul 7.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Rd, Haidian District, Beijing, 100085, China.

Ferroptosis provide new insights into designing nanomedicines for enhanced cancer therapy; however, its antitumor efficacy is relatively low, mainly due to self-protective mechanism of cancer cells, , heat shock protein (HSP) overexpression. Since HSPs can be modified/inhibited by lipid peroxidation (LPO) ending products, we construct a nanoplatform, namely [email protected], to amplify intracellular reactive oxygen species (ROS) and LPO for synergistic ferrotherapy. Upon tumor acidic microenvironment and local near-infrared stimuli, this nanoplatform releases FeO and reacts with intracellular hydrogen peroxide (HO) to promote Fenton reaction, and yields significant intracellular ROS (specifically hydroxyl radical, OH) and LPO. In turn, LPO ending products crosslink HSPs to destroy self-preservation pathways of cancer cells to enhance anticancer effect. Meanwhile, the released erastin inhibits system X signal pathway to depletes glutathione. FeO loading further provides magnetic resonance imaging T2-weighted signal to guide anti-tumor treatment. Together, this nanoplatform not only provides OH (as a "sword" to attack tumor cells), but also inhibits system X signal pathway and crosslinks HSP (break down the "shield" of tumor cells) to maximize synergistic ferro-therapeutic effect. [email protected] plus laser irradiation possessed highly efficient tumor suppression with magnified the levels of OH and inactive glutathione peroxidase 4 (GPX4), which can promote the development of precise cooperative cancer therapy.
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http://dx.doi.org/10.1016/j.mtbio.2022.100353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294558PMC
December 2022

Response of Source-Sink Characteristics and Rice Quality to High Natural Field Temperature During Reproductive Stage in Irrigated Rice System.

Front Plant Sci 2022 5;13:911181. Epub 2022 Jul 5.

National Key Laboratory of Crop Genetic Improvement, MOA Key Laboratory of Crop Physiology, Ecology and Cultivation (The Middle Reaches of Yangtze River), Huazhong Agricultural University, Wuhan, China.

Global warming greatly affects the development of rice at different growth stages, thereby deteriorating rice quality. However, the effect of high natural field temperature during reproductive stages on rice quality is unclear. Thus, grain filling dynamics, source-sink characteristics and quality-related traits were compared between two contrasting natural field temperature conditions namely normal (low temperature) (LRT) and hot (high temperature) growth season (HRT) during reproductive stage. Compared with LRT, HRT significantly increased chalky grain rate (about 1.6-3.1%), chalkiness level (about 4.7-22.4%), protein content (about 0.93-1.07%), pasting temperature, setback, and consistence, and decreased total starch content (about 4.6-6.2%). Moreover, HRT significantly reduced the leaf area index (LAI, about 0.54-1.11 m m), specific leaf weight (SLW, about 1.27-1.44 mg cm) and source-sink ratio (leaf-sink ratio and/or stem-sink ratio), shortened the active grain filling period by 3.1-3.2 days, and reduced the rations of dry matter translocation to grain (RDMs). In conclusion, we suggested that significant reduction in assimilate translocation after flowering, resulting in the reduced active grain-filling duration and the poor rice quality (high chalkiness and the poor eating and cooking quality), modulated by source-sink characteristics in response to high natural field temperature during reproductive stage. These results enriched the study of high temperature-stressed rice and served as an important reference for selecting high-quality, heat-tolerant varieties and protecting rice quality under high-temperature conditions.
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http://dx.doi.org/10.3389/fpls.2022.911181DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294507PMC
July 2022

Safety-Related Drug Withdrawals in China Between 1999 and 2021: A Systematic Investigation and Analysis.

Drug Saf 2022 Jul 12;45(7):737-745. Epub 2022 Jul 12.

School of Pharmaceutical Sciences, Tsinghua University, Beijing, China.

Introduction: The Chinese National Medical Products Administration (NMPA) has emphasized proactive pharmacovigilance throughout the product life cycle in recent years. However, the safety-related withdrawal of drugs from the Chinese market has received less attention.

Objectives: The primary aim of the study was to investigate the context of withdrawing a drug for safety reasons in China (between 1999 and 2021).

Methods: Withdrawn drugs were first identified from the Chinese NMPA and United States (US) Food and Drug Administration websites and the World Health Organization's (WHO's) consolidated list of products, WHO Drug Information, and WHO Pharmaceuticals Newsletter. We then searched the China National Knowledge Internet database, Chongqing VIP information database, Wanfang database, PubMed, and Google Scholar for drug withdrawal details. We used the Oxford Centre for Evidence-Based Medicine criteria to assess the levels of evidence that support withdrawing a drug.

Results: A total of 30 drugs were withdrawn from the Chinese market between 1999 and 2021. The number of withdrawals increased during the stable Chinese drug surveillance period (2012-2021). Evidence from case-series or case-control studies was primarily used to determine the withdrawals of 16 drugs (53.3%). Fifteen drugs were withdrawn from the markets of China and the US, including five drugs (5/15, 33.3%) that were withdrawn in the same year in China and the US.

Conclusions: The promulgation of regulations and development of advanced passive and active systems have enhanced pharmacovigilance in China. High-quality evidence, coordination with other regulatory authorities, and communication and information sharing should be strengthened to optimize drug safety surveillance and risk management.
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http://dx.doi.org/10.1007/s40264-022-01185-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296427PMC
July 2022

Human Behavior Recognition in Outdoor Sports Based on the Local Error Model and Convolutional Neural Network.

Comput Intell Neurosci 2022 28;2022:6988525. Epub 2022 Jun 28.

ZUGO Intelligence Technology (Shen Zhen) Co. Ltd., ZUGO Digital Energy Building Keji First Road High-Tech Zone, Zhuhai, Guandong 519080, China.

With the rapid development of the Internet, various electronic products based on computer vision play an increasingly important role in people's daily lives. As one of the important topics of computer vision, human action recognition has become the main research hotspot in this field in recent years. The human motion recognition algorithm based on the convolutional neural network can realize the automatic extraction and learning of human motion features and achieve good classification performance. However, deep convolutional neural networks usually have a large number of layers, a large number of parameters, and a large memory footprint, while embedded wearable devices have limited memory space. Based on the traditional cross-entropy error-based training mode, the parameters of all hidden layers must be kept in memory and cannot be released until the end of forward and reverse error propagation. As a result, the memory used to store the parameters of the hidden layer cannot be released and reused, and the memory utilization efficiency is low, which leads to the backhaul locking problem, limiting the deployment and execution of deep convolutional neural networks on wearable sensor devices. Based on this, this topic designs a local error convolutional neural network model for human motion recognition tasks. Compared with the traditional global error, the local error constructed in this paper can train the convolutional neural network layer by layer, and the parameters of each layer can be trained independently according to the local error and does not depend on the gradient propagation of adjacent upper and lower layers. As a result, the memory used to store all hidden layer parameters can be released in advance without waiting for the end of forward and backward propagation, avoiding the problem of backhaul locking, and improving the memory utilization of convolutional neural networks deployed on embedded wearable devices.
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http://dx.doi.org/10.1155/2022/6988525DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256384PMC
July 2022

Diagnostic Value of Spectral-Domain Optical Coherence Tomography for Polypoidal Choroidal Vasculopathy: A Systematic Review and Meta-Analysis.

Front Med (Lausanne) 2022 15;9:878946. Epub 2022 Jun 15.

Department of Ophthalmology, Tianjin Baodi Hospital, Tianjin, China.

Purpose: To evaluate the diagnostic value of spectral-domain optical coherence tomography (SD-OCT) for polypoidal choroidal vasculopathy (PCV).

Methods: A search of electronic databases was conducted from 2010 to 2021 to review the relevant literature on SD-OCT to identify PCV and other lesions causing serious or serosanguinous retinal pigment epithelial detachment (PED), specifically neovascular age-related macular degeneration (nvAMD). The QUADAS-2 scale was used to evaluate the quality of the literature. We performed a meta-analysis, including heterogeneity tests, analyze and synthesize the study data, meta-regression analysis, subgroup analysis, Fagan's plot, sensitivity analysis and publication bias tests.

Results: A total of 12 related studies involving 1,348 eyes were included in this study, and the random-effects model was used for meta-analysis. The results showed that the pooled sensitivity of SD-OCT in the diagnosis of PCV was 0.87 (95% CI: 0.84-0.89), the pooled specificity was 0.83 (95% CI: 0.80-0.86), and the pooled positive/negative likelihood ratios were 5.38 (95% CI: 3.28-8.80) and 0.16 (95% CI: 0.10-0.25), respectively. The diagnostic odds ratio (DOR) was 36.07 (95% CI: 15.98-81.40), and the area under the sROC curve (AUC) was 0.9429. When the pre-test probability was set at 20%, the post-test positive and negative probabilities were 58% and 4%, respectively. Meta-regression indicated that race was the primary source of heterogeneity (P <0.05). The Deeks' funnel plot showed no significant publication bias in this study (P>0.05).

Conclusion: SD-OCT has high sensitivity and specificity for the diagnosis of PCV, as well as significant clinical applicability. Since color fundus photography (CFP) is more clinically available and can improve the diagnostic efficacy, we recommend SD-OCT combined with CFP to diagnose PCV, especially without indocyanine green angiography (ICGA).

Systematic Review Registration: https://inplasy.com/inplasy-2021-12-0048/, identifier: INPLASY2021120048.
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http://dx.doi.org/10.3389/fmed.2022.878946DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9242399PMC
June 2022

mA demethylase ALKBH5 is required for antibacterial innate defense by intrinsic motivation of neutrophil migration.

Signal Transduct Target Ther 2022 06 29;7(1):194. Epub 2022 Jun 29.

Department of Immunology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, 100005, Beijing, China.

Neutrophil migration into the site of infection is necessary for antibacterial innate defense, whereas impaired neutrophil migration may result in excessive inflammation and even sepsis. The neutrophil migration directed by extracellular signals such as chemokines has been extensively studied, yet the intrinsic mechanism for determining neutrophil ability to migrate needs further investigation. N-methyladenosine (mA) RNA modification is important in immunity and inflammation, and our preliminary data indicate downregulation of RNA mA demethylase alkB homolog 5 (ALKBH5) in neutrophils during bacterial infection. Whether mA modification and ALKBH5 might intrinsically modulate neutrophil innate response remain unknown. Here we report that ALKBH5 is required for antibacterial innate defense by enhancing intrinsic ability of neutrophil migration. We found that deficiency of ALKBH5 increased mortality of mice with polymicrobial sepsis induced by cecal ligation and puncture (CLP), and Alkbh5-deficient CLP mice exhibited higher bacterial burden and massive proinflammatory cytokine production in the peritoneal cavity and blood because of less neutrophil migration. Alkbh5-deficient neutrophils had lower CXCR2 expression, thus exhibiting impaired migration toward chemokine CXCL2. Mechanistically, ALKBH5-mediated mA demethylation empowered neutrophils with high migration capability through altering the RNA decay, consequently regulating protein expression of its targets, neutrophil migration-related molecules, including increased expression of neutrophil migration-promoting CXCR2 and NLRP12, but decreased expression of neutrophil migration-suppressive PTGER4, TNC, and WNK1. Our findings reveal a previously unknown role of ALKBH5 in imprinting migration-promoting transcriptome signatures in neutrophils and intrinsically promoting neutrophil migration for antibacterial defense, highlighting the potential application of targeting neutrophil mA modification in controlling bacterial infections.
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http://dx.doi.org/10.1038/s41392-022-01020-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240034PMC
June 2022

Clinicopathologic significance of expression of Her-2 and P53 in gastric cancer.

Asian J Surg 2022 Jun 24. Epub 2022 Jun 24.

Department of Gastrointestinal, Colorectal, and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, China. Electronic address:

Background: To detect the expression of HER-2 and P53 patients with gastric cancer and to analyze their correlation.

Methods: A total of 249 gastric cancer patients with complete clinical data who received surgical treatment from China-Japan Union Hospital of Jilin University were selected. The expression of Her-2 and P53 were detected by immunohistochemistry using the streptavidin-biotin-peroxidase method. The correlations between HER-2 and P53 in gastric cancer were analyzed.

Results: The positive rate of Her-2 and P53 expression was 37.3% (93/249) and 100% in all the specimens, respectively. The intensity of Her-2 expression was significantly different in patients with different degrees of gastric cancer cell differentiation (P = 0.012). Meanwhile, the expression of her-2 was closely related to whether the pathological type of gastric cancer was a signet-ring cell carcinoma (P = 0.022). Different percentage of positive P53 expression was closely related to the grade of tumor differentiation (P = 0.035) and positive Ki67 expression (P = 0.001). There was a significant positive correlation between HER-2 and P53 expression in gastric cancer (P = 0.003). These findings suggest that HER-2 and P53 have synergistic effects in gastric cancer.

Conclusion: Her-2 and P53 are important markers for invasion and metastasis of gastric cancer. Combined detection of P53 and Her-2 expression in gastric cancer tissue can be used to assess prognosis and screen cancer patients at high risk of metastasis.
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http://dx.doi.org/10.1016/j.asjsur.2022.06.039DOI Listing
June 2022

Amyloid-PET Levels in the Precuneus and Posterior Cingulate Cortices Are Associated with Executive Function Scores in Preclinical Alzheimer's Disease Prior to Overt Global Amyloid Positivity.

J Alzheimers Dis 2022 ;88(3):1127-1135

Sanders-Brown Center on Aging, College of Medicine, University of Kentucky, Lexington, KY, USA.

Background: Global amyloid-β (Aβ) deposition in the brain can be quantified by Aβ-PET scans to support or refute a diagnosis of preclinical Alzheimer's disease (pAD). Yet, Aβ-PET scans enable quantitative evaluation of regional Aβ elevations in pAD, potentially allowing even earlier detection of pAD, long before global positivity is achieved. It remains unclear as to whether such regional changes are clinically meaningful.

Objective: Test the hypothesis that early focal regional amyloid deposition in the brain is associated with cognitive performance in specific cognitive domain scores in pAD.

Methods: Global and regional standardized uptake value ratios (SUVr) from 18F-florbetapir PET/CT scanning were determined using the Siemens Syngo.via® Neurology software package across a sample of 99 clinically normal participants with Montreal Cognitive Assessment (MoCA) scores≥23. Relationships between regional SUVr and cognitive test scores were analyzed using linear regression models adjusted for age, sex, and education. Participants were divided into two groups based on SUVr in the posterior cingulate and precuneus gyri (SUVR≥1.17). Between group differences in cognitive test scores were analyzed using ANCOVA models.

Results: Executive function performance was associated with increased regional SUVr in the precuneus and posterior cingulate regions only (p < 0.05). There were no significant associations between memory and Aβ-PET SUVr in any regions of the brain.

Conclusion: These data demonstrate that increased Aβ deposition in the precuneus and posterior cingulate (the earliest brain regions affected with Aβ pathology) is associated with changes in executive function that may precede memory decline in pAD.
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http://dx.doi.org/10.3233/JAD-220294DOI Listing
August 2022

Demonstration of SWIR Silicon-Based Photodetection by Using Thin ITO/Au/Au Nanoparticles/n-Si Structure.

Sensors (Basel) 2022 Jun 16;22(12). Epub 2022 Jun 16.

Key Laboratory for Renewable Energy, Beijing Key Laboratory for New Energy Materials and Devices, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.

Plasmonic photodetection based on the hot-electron generation in nanostructures is a promising strategy for sub-band detection due to the high conversion efficiencies; however, it is plagued with the high dark current. In this paper, we have demonstrated the plasmonic photodetection with dark current suppression to create a Si-based broadband photodetector with enhanced performance in the short-wavelength infrared (SWIR) region. By hybridizing a 3 nm Au layer with the spherical Au nanoparticles (NPs) formed by rapid thermal annealing (RTA) on Si substrate, a well-behaved ITO/Au/Au NPs/n-Si Schottky photodetector with suppressed dark current and enhanced absorption in the SWIR region is obtained. This optimized detector shows a broad detection beyond 1200 nm and a high responsivity of 22.82 mA/W at 1310 nm at -1 V, as well as a low dark current density on the order of 10 A/cm. Such a Si-based plasmon-enhanced detector with desirable performance in dark current will be a promising strategy for realization of the high SNR detector while keeping fabrication costs low.
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http://dx.doi.org/10.3390/s22124536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229662PMC
June 2022

Current status of carbon neutrality in Chinese rice fields (2002-2017) and strategies for its achievement.

Sci Total Environ 2022 Jun 14;842:156713. Epub 2022 Jun 14.

Macro Research Agricultural Institute, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

China has pledged to achieve carbon neutrality by 2060 to address global climate change, and achieving carbon neutrality in rice fields is a vital component of this commitment. However, the current status of carbon neutrality in rice fields in China is unclear, and there are few feasible strategies to achieve its successful implementation. Therefore, this study calculated the net carbon sequestration rate (NCSR, i.e., carbon sequestration minus carbon emissions) of rice fields in China from 2002 to 2017 to clarify the carbon neutrality status of Chinese rice fields. Furthermore, the effects of field management measures, rice sown area, and rice yield on NCSR were analyzed to identify suitable carbon neutralization pathways in Chinese rice fields. Our findings indicated that the annual carbon sequestration rate in rice fields was lower than the carbon emissions, resulting in continuous net emissions of 195.49 Tg CO-eq yr. The NCSR of paddy fields increased first and then decreased with increases in rice sown area and yield. Meta-analysis indicated that management measures such as water conservation and biochar significantly increased NCSR by ~5766.50 kg CO-eq ha yr and 22,296.62 kg CO-eq ha yr, respectively. Our findings suggests that proper control of rice sown area and the adoption of reasonable field management measures (water conservation and biochar) can promote carbon neutrality in Chinese rice fields.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156713DOI Listing
June 2022

Realizing Single Chip White Light InGaN LED via Dual-Wavelength Multiple Quantum Wells.

Materials (Basel) 2022 Jun 3;15(11). Epub 2022 Jun 3.

Key Laboratory for Renewable Energy, Beijing Key Laboratory for New Energy Materials and Devices, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.

Dual-wavelength multiple quantum wells (MQWs) have great potential in realizing high quality illumination, monolithic micro light-emitting diode (LED) displays and other related fields. Here, we demonstrate a single chip white light indium gallium nitride (InGaN) LED via the manipulation of the dual-wavelength MQWs. The MQWs contain four pairs of blue light-emitting MQWs and one pair of green light-emitting QW. The fabricated LED chips with nickel/gold (Ni/Au) as the current spreading layer emit white light with the injection current changing from 0.5 mA to 80 mA. The chromaticity coordinates of (0.3152, 0.329) closing to the white light location in the Commission International de I'Eclairage (CIE) 1931 chromaticity diagram are obtained under a 1 mA current injection with a color rendering index (CRI) Ra of 60 and correlated color temperature (CCT) of 6246 K. This strategy provides a promising route to realize high quality white light in a single chip, which will significantly simplify the production process of incumbent white light LEDs and promote the progress of high-quality illumination.
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http://dx.doi.org/10.3390/ma15113998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181966PMC
June 2022

Universal protein misfolding intermediates can bypass the proteostasis network and remain soluble and less functional.

Nat Commun 2022 06 2;13(1):3081. Epub 2022 Jun 2.

Department of Chemistry, Pennsylvania State University, University Park, PA, 16802, USA.

Some misfolded protein conformations can bypass proteostasis machinery and remain soluble in vivo. This is an unexpected observation, as cellular quality control mechanisms should remove misfolded proteins. Three questions, then, are: how do long-lived, soluble, misfolded proteins bypass proteostasis? How widespread are such misfolded states? And how long do they persist? We address these questions using coarse-grain molecular dynamics simulations of the synthesis, termination, and post-translational dynamics of a representative set of cytosolic E. coli proteins. We predict that half of proteins exhibit misfolded subpopulations that bypass molecular chaperones, avoid aggregation, and will not be rapidly degraded, with some misfolded states persisting for months or longer. The surface properties of these misfolded states are native-like, suggesting they will remain soluble, while self-entanglements make them long-lived kinetic traps. In terms of function, we predict that one-third of proteins can misfold into soluble less-functional states. For the heavily entangled protein glycerol-3-phosphate dehydrogenase, limited-proteolysis mass spectrometry experiments interrogating misfolded conformations of the protein are consistent with the structural changes predicted by our simulations. These results therefore provide an explanation for how proteins can misfold into soluble conformations with reduced functionality that can bypass proteostasis, and indicate, unexpectedly, this may be a wide-spread phenomenon.
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http://dx.doi.org/10.1038/s41467-022-30548-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163053PMC
June 2022

Glioblastoma-associated microglia-derived exosomal circKIF18A promotes angiogenesis by targeting FOXC2.

Oncogene 2022 06 30;41(26):3461-3473. Epub 2022 May 30.

Department of Neurosurgery, The First Hospital of China Medical University, NO. 155 North Nanjing Street, Shenyang, 110001, China.

Glioblastoma multiforme (GBM) is the most lethal primary tumor with active neovascularization in the central nervous system. Studying the novel molecular mechanisms of GBM angiogenesis is very important. The glioblastoma-associated microglia (GAM) M2 polarization was constructed, and microglia-derived exosomes (MDEs) were isolated to co-culture with human brain microvessel endothelial cells (hBMECs). CircRNA sequence and molecular biological experiments were used to detect the expression levels and regulation functions among circKIF18A, FOXC2, ITGB3, CXCR4, DLL4 and the PI3K/AKT signaling. The functional effects of silencing or overexpression of these molecules were evaluated in hBMECs viability, invasion, and tube formation in vitro and tumorigenicity in vivo. M2 microglia polarization is positively correlated with microvessels' density in GBM patients. M2 GAM can promote the angiogenesis of GBM via transporting exosomal circKIF18A into hBMECs. Mechanistically, circKIF18A can bind to, maintain the stability and nuclear translocation of FOXC2 in hBMECs. Furtherly, as a transcription factor, FOXC2 can directly bind to the promoter of ITGB3, CXCR4, and DLL4 and upregulate their expressions. Besides, FOXC2 can also activate the PI3K/AKT signaling and promote the angiogenesis of GBM. Our study identified a novel molecular mechanism for M2 GAM-derived exosomal circKIF18A participating in GBM angiogenesis via targeting FOXC2. This may provide a novel treatment target to improve the outcomes for anti-angiogenic therapies in GBM.
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http://dx.doi.org/10.1038/s41388-022-02360-4DOI Listing
June 2022

One-Step Preparation of Adhesive Composite Hydrogels through Fast and Simultaneous In Situ Formation of Silver Nanoparticles and Crosslinking.

Gels 2022 Apr 21;8(5). Epub 2022 Apr 21.

College of Materials and Textile Engineering & Nanotechnology Research Institute (NRI), Jiaxing University, Jiaxing 314001, China.

In this study, a series of gelatin/silver nanoparticles (AgNPs) composite hydrogels are prepared for the first time through the facile in situ formation of AgNPs. AgNPs, which are formed by reducing Ag using dopamine-conjugated gelatins. These can simultaneously crosslink gelatin molecules, thus generating three-dimentional and porous hydrogels. The gelation time and pore sizes of these composite hydrogels can be controlled by controlling the feeding concentration of AgNO and weight content of gelatin in water, respectively. The feeding concentration of AgNO also has an effect on the equilibrium swelling ratio of the hydrogels. Moreover, these composite hydrogels, with a controllable gelation time and in situ forming ability, exhibit good adhesive properties and can be used as drug-release depots.
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http://dx.doi.org/10.3390/gels8050256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141602PMC
April 2022

A Bioinspired Iron-Molybdenum μ-Nitrido Complex and Its Reactivity toward Ammonia Formation.

Angew Chem Int Ed Engl 2022 07 7;61(30):e202203121. Epub 2022 Jun 7.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian, 116024, China.

Multimetallic nitride species, especially those containing biologically related iron or molybdenum, are fundamentally important to understand the nitrogen reduction process catalysed by FeMo-nitrogenase. However, until now, there remains no report about the construction of structurally well-defined FeMo heteronuclear nitrido complex and its reactivity toward ammonia formation. Herein, a novel thiolate-bridged Fe Mo complex featuring a bent Fe-N≡Mo fragment is synthesized and structurally characterized, which can be easily protonated to form a μ-imido complex. Subsequently, through the proton-coupled electron transfer (PCET) process, this imido species can smoothly convert into the μ-amido complex, which can further undergo reductive protonation to afford the FeMo complex containing an ammine ligand. Overall, we present the first well-defined {Fe(μ-S) Mo} platform that can give a panoramic picture for the late stage (N →NH →NH →NH ) of biological nitrogen reduction by the heterometallic cooperativity.
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http://dx.doi.org/10.1002/anie.202203121DOI Listing
July 2022

N-polar GaN Film Epitaxy on Sapphire Substrate without Intentional Nitridation.

Materials (Basel) 2022 Apr 21;15(9). Epub 2022 Apr 21.

Key Laboratory for Renewable Energy, Beijing Key Laboratory for New Energy Materials and Devices, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.

High-temperature nitridation is commonly thought of as a necessary process to obtain N-polar GaN films on a sapphire substrate. In this work, high-quality N-polar GaN films were grown on a vicinal sapphire substrate with a 100 nm high-temperature (HT) AlN buffer layer (high V/III ratio) and without an intentional nitriding process. The smallest X-ray full width at half maximum (FWHM) values of the (002)/(102) plane were 237/337 arcsec. On the contrary, N-polar GaN film with an intentional nitriding process had a lower crystal quality. In addition, we investigated the effect of different substrate treatments 1 min before the high-temperature AlN layer's growth on the quality of the N-polar GaN films grown on different vicinal sapphire substrates.
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http://dx.doi.org/10.3390/ma15093005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103289PMC
April 2022

Treatment effects on event-related EEG potentials and oscillations in Alzheimer's disease.

Int J Psychophysiol 2022 Jul 16;177:179-201. Epub 2022 May 16.

Sapienza University of Rome, Department of Physiology and Pharmacology "V. Erspamer", Rome, Italy; San Raffaele of Cassino, Cassino, Italy.

Alzheimer's disease dementia (ADD) is the most diffuse neurodegenerative disorder belonging to mild cognitive impairment (MCI) and dementia in old persons. This disease is provoked by an abnormal accumulation of amyloid-beta and tauopathy proteins in the brain. Very recently, the first disease-modifying drug has been licensed with reserve (i.e., Aducanumab). Therefore, there is a need to identify and use biomarkers probing the neurophysiological underpinnings of human cognitive functions to test the clinical efficacy of that drug. In this regard, event-related electroencephalographic potentials (ERPs) and oscillations (EROs) are promising candidates. Here, an Expert Panel from the Electrophysiology Professional Interest Area of the Alzheimer's Association and Global Brain Consortium reviewed the field literature on the effects of the most used symptomatic drug against ADD (i.e., Acetylcholinesterase inhibitors) on ERPs and EROs in ADD patients with MCI and dementia at the group level. The most convincing results were found in ADD patients. In those patients, Acetylcholinesterase inhibitors partially normalized ERP P300 peak latency and amplitude in oddball paradigms using visual stimuli. In these same paradigms, those drugs partially normalize ERO phase-locking at the theta band (4-7 Hz) and spectral coherence between electrode pairs at the gamma (around 40 Hz) band. These results are of great interest and may motivate multicentric, double-blind, randomized, and placebo-controlled clinical trials in MCI and ADD patients for final cross-validation.
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http://dx.doi.org/10.1016/j.ijpsycho.2022.05.008DOI Listing
July 2022

Acetylation of Sesquiterpenyl Epoxy-Cyclohexenoids Regulates Fungal Growth, Stress Resistance, Endocytosis, and Pathogenicity of Nematode-Trapping Fungus via Metabolism and Transcription.

J Agric Food Chem 2022 May 12;70(20):6145-6155. Epub 2022 May 12.

State Key Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming 650091, People's Republic of China.

Sesquiterpenyl epoxy-cyclohexenoids (SECs) that depend on a polyketide synthase-terpenoid synthase (PKS-TPS) pathway are widely distributed in plant pathogenic fungi. However, the biosynthesis and function of the acetylated SECs still remained cryptic. Here, we identified that 00215 273 (273) was responsible for the acetylation of SECs in via the construction of Δ273, in which the acetylated SECs were absent and major antibacterial nonacetylated SECs accumulated. Mutant Δ273 displayed increased trap formation, and nematicidal and antibacterial activities but decreased fungal growth and soil colonization. Glutamine, a key precursor for NH as a trap inducer, was highly accumulated, and biologically active phenylpropanoids and antibiotics were highly enriched in Δ273. The decreased endocytosis and increased autophagosomes, with the most upregulated genes involved in maintaining DNA and transcriptional stability and pathways related to coronavirus disease and exosome, suggested that lack of 273 might result in increased virus infection and the acetylation of SECs played a key role in fungal diverse antagonistic ability.
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http://dx.doi.org/10.1021/acs.jafc.2c01914DOI Listing
May 2022

MiR-34a-5p/Sirt1 axis: A novel pathway for puerarin-mediated hepatoprotection against benzo(a)pyrene.

Free Radic Biol Med 2022 Jun 10;186:53-65. Epub 2022 May 10.

College of Food Science and Engineering, Shandong Agricultural University, Key Laboratory of Food Processing Technology and Quality Control of Shandong Higher Education Institutes, Taian, 271018, People's Republic of China. Electronic address:

Benzo[a]pyrene (BaP) as a carcinogen induces oxidative stress and inflammation, causing health problems including liver damage. Puerarin (a natural flavonoid) is traditionally used to provide hepatoprotective effects. This research was established to meet the rising demand for effective therapies/treatments against hepatic diseases and investigate the mechanism underlying the protective actions of puerarin against BaP-induced liver damage. In mice, puerarin combated effectively the detrimental changes in liver weight, color and function indices caused by BaP. In HepG2 cells, puerarin alleviated BaP-induced cell death, oxidative stress and inflammation, and such effects were positively correlated with puerarin's concentration (12.5-50 μM). Mechanistic studies revealed that BaP induced low Sirt1 expression and high miR-34a-5p expression, and puerarin treatment alleviated these changes. Oxidative stress and inflammation induced by BaP were almost eliminated when miR-34a-5p was silenced. Inhibiting miR-34a-5p or overexpressing Sirt1 had a similar effect to puerain treatment. Overexpression of miR-34a-5p and inhibition of Sirt1 reduced the protective effect of puerarin. Collectively, miR-34a-5p participates in the regulation of puerarin's protective function against BaP-induced injury through targeting Sirt1. There is a novel pathway for suppressing oxidative stress and inflammation via miR-34a-5p/Sirt1 axis in puerarin-mediated hepatoprotection, which opens up a new avenue for alternative therapies.
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http://dx.doi.org/10.1016/j.freeradbiomed.2022.05.006DOI Listing
June 2022

Highly crystallized glass-ceramics from high content gold tailings a one-step direct cooling method.

RSC Adv 2022 May 11;12(22):14175-14182. Epub 2022 May 11.

School of Materials Science and Engineering, Hefei University of Technology Hefei 230009 China

Highly crystalline glass-ceramics were successfully manufactured a one-step direct cooling method using Shuangqishan (Fujian, China) gold tailings as raw materials. A series of glass-ceramics were prepared by controlling the gold tailings addition and post-treatment. X-ray diffraction results show that the crystalline phase of glass-ceramics samples with high tailing addition content (65-80 wt%) is akermanite phase (CaMgSiO). By contrast, the main phase of 60 wt% and 55 wt% tailings addition samples is diopside (CaMgSiO) crystalline phase. In addition, although glass-ceramics have typical fracture characteristics of brittle materials, the crack propagation in the fracture process is disturbed by grains, resulting in the deviation of the fracture path in terms of macroscopic and microcosmic observation. Based on the investigation of samples with different tailings additions, glass-ceramics with 60 wt% tailings contents show excellent mechanical properties with a density of 2.89 g cm, a Vickers hardness value of 8.17 GPa, and a flexural strength of 116 MPa after 950 °C heat treatment. This study further confirms the possibility of using Shuangqi Mountain gold tailings as the raw materials for highly crystalline glass-ceramics, which shows great potential for application in mass production.
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http://dx.doi.org/10.1039/d2ra01012gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092354PMC
May 2022

High performance visible-SWIR flexible photodetector based on large-area InGaAs/InP PIN structure.

Sci Rep 2022 May 10;12(1):7681. Epub 2022 May 10.

Key Laboratory for Renewable Energy, Beijing Key Laboratory for New Energy Materials and Devices, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, 100190, China.

Mechanically flexible optoelectronic devices and systems can enable a much broader range of applications than what their rigid counterparts can do, especially for novel bio-integrated optoelectronic systems, flexible consumer electronics and wearable sensors. Inorganic semiconductor could be a good candidate for the flexible PD when it can keep its high performance under the bending condition. Here, we demonstrate a III-V material-based flexible photodetector operating wavelength from 640 to 1700 nm with the high detectivity of 5.18 × 10 cm‧Hz/W and fast response speed @1550 nm by using a simply top-to-down fabrication process. The optoelectrical performances are stable as the PDs are exposed to bending cycles with a radius of 15 mm up to 1000 times. Furthermore, the mechanical failure mode of the PD is also investigated, which suggests that the cracking and delamination failure mode are dominant in bending up and bending down direction, respectively. Such a flexible III-V material-based PD and design with stable and high performance could be a promising strategy for the application of the flexible broad spectrum detection.
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http://dx.doi.org/10.1038/s41598-022-11946-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9090829PMC
May 2022

Detection and typing of dengue virus by one-step RT-PCR-based high-resolution melting assay.

Virus Genes 2022 Aug 10;58(4):319-326. Epub 2022 May 10.

Shangcheng District Center for Disease Control and Prevention, Hangzhou, China.

Dengue is a rapidly spreading arboviral disease that can be transmitted through any of the four types of dengue virus (DENV) serotypes. Previous studies have observed that individuals who have a pre-existing secondary infection due to a different dengue serotype, experience severe forms of this disease. During a DENV outbreak, a time-sensitive preliminary diagnosis of the origin of DENV might be useful in controlling the epidemic. Here, we developed a rapid and accurate one-step TB Green RT-PCR-based high-resolution melting (HRM) assay to identify and serotype DENV using serotyping primers based on the alignment with the E gene. This assay had a detection limit of 7.7 × 10 (DENV 1), 3.8 × 10 (DENV 2), 6.2 × 10 (DENV 3), and 1.2 × 10 (DENV 4) RNA copies/mL. No cross-reactivity with the Chikungunya, Zika, and Japanese encephalitis viruses was observed. The feasibility of using this assay for clinical diagnosis was evaluated in DENV-positive patient sera. The HRM assay and the RT-qPCR had complete matched results derived from DENV detection, including 51 serum positive and 20 serum negative. Additionally, eight DENV 2 strains in the same serotype were successfully differentiated by an HRM assay. Thus, this assay facilitated accurate detection and serotyping of DENV, along with the time-sensitive identification of the infectious focus of different DENVs.
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http://dx.doi.org/10.1007/s11262-022-01906-8DOI Listing
August 2022
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