Publications by authors named "Yang Jiang"

632 Publications

A New Modified Hysteroscopic-Laparoscopic Surgery for Cesarean Scar Pregnancy of Stable Type III.

Int J Gen Med 2021 3;14:2289-2295. Epub 2021 Jun 3.

Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Hubei, People's Republic of China.

Objective: To introduce a modified hysteroscopic-laparoscopic operation for cesarean scar pregnancy (CSP) of stable type III.

Patients And Methods: We retrospectively studied the case notes of 31 patients with stable type III cesarean scar pregnancy who underwent hysteroscopic-laparoscopic surgery in our hospital. Thirteen patients received the modified hysteroscopic-laparoscopic surgery (modified surgery group), and eighteen patients received traditional hysteroscopic-laparoscopic surgery (traditional surgery group).

Results: There was no significant difference in patients' age, gestational age, number of previous cesarean sections, the serum human chorionic gonadotropin (hCG) level before surgery, gestational sac diameter, myometrium thickness between the two groups. In the modified hysteroscopic-laparoscopic surgery, the mean surgical time was 50.45±24.45 mins, the mean length of stay in hospital was 4.50±0.50 days, which was significantly shorter than the traditional surgery group (84.75±33.28 mins and 5.50±0.75 days, respectively). And the intraoperative hemorrhage in the modified group was also less than that in the traditional group (40.50±12.25 mL vs 75.33±25.45mL). Whereas the time for hCG normalization, postoperative vaginal bleeding and menstrual recovery had no significant difference between the modified surgery group and the traditional surgery group. There was no recurrence of CSP in both groups.

Conclusion: The modified hysteroscopic-laparoscopic surgery had shorter operation time, less blood loss, and sooner recovery time after surgery compared to traditional hysteroscopic-laparoscopic surgery, which could be more beneficial to our patients and should be applied in clinics generally.
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http://dx.doi.org/10.2147/IJGM.S308768DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184249PMC
June 2021

Using Sankey diagrams to visualize drag and drop action sequences in technology-enhanced items.

Behav Res Methods 2021 Jun 9. Epub 2021 Jun 9.

Educational Testing Service, Princeton, NJ, USA.

We propose a Sankey diagram-based visualization method to illustrate test-takers' action sequences in technology-enhanced, drag-and-drop items. This method extends existing studies focusing only on initial or last attempt(s) of answer formulation, and extracts, at the item level, comprehensive information of test-takers' drag-and-drop actions. Using the process data from three mathematical items in the National Assessment of Educational Progress, we exemplify how to develop Sankey diagrams, discover frequent actions at each stage of problem solving, deduce test-takers' response strategies, and discuss the relationship among domain (i.e., mathematics) knowledge, response strategy, and item performance. The applications and future extensions of this method can contribute to process-based problem-solving research in educational assessments.
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http://dx.doi.org/10.3758/s13428-021-01615-4DOI Listing
June 2021

PDCD4 negatively regulated osteogenic differentiation and bone defect repair of mesenchymal stem cells through GSK-3β/β-catenin pathway.

Stem Cells Dev 2021 Jun 4. Epub 2021 Jun 4.

The Second Hospital, Cheeloo College of Medicine, Shandong University, Department of Hematology, jinan, shandong, China;

Mesenchymal stem cells (MSCs) have been shown to be involved in bone injury repair. Programmed cell death 4 (PDCD4) is a tumor suppressor gene, but also plays roles in the regulation of MSCs function. The aim of the study was to uncover PDCD4 potential regulatory roles and mechanisms in the osteogenic differentiation and bone defect repair of MSCs. shRNA technique was used to knockdown PDCD4 expression in umbilical cord-derived MSCs (shPDCD4-UCMSCs). Their phenotype was characterized by flow cytometry and the differentiation potential was verified. We found that PDCD4 knockdown did not affect the surface molecule expression of UCMSCs, but significantly enhanced their osteogenic differentiation and osteogenesis-related molecule expression. Mechanistically, glycogen synthase kinase-3β (GSK-3β) phosphorylation and β-catenin expression were significantly increased in shPDCD4-UCMSCs during the osteogenic differentiation process. The β-catenin inhibitor PNU-74654 reversed shPDCD4-increased osteogenesis and osteogenesis-related molecule expression. The results of animal experiments showed that shPDCD4-UCMSCs markedly improved the defect healing in rabbits. Our findings suggest that PDCD4 acts as a negative regulator of MSC osteogenic differentiation through GSK-3β/β-catenin pathway. Targeting PDCD4 may be a way to improve MSC-mediated therapeutic effects on bone injury.
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http://dx.doi.org/10.1089/scd.2021.0041DOI Listing
June 2021

Developing and Assessing Nonbonded Dummy Models of Magnesium Ion with Different Hydration Free Energy References.

J Chem Inf Model 2021 Jun 3. Epub 2021 Jun 3.

Department of Biological Science and Engineering, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 100083 Beijing, China.

A large diversity in the targeted hydration free energies (HFEs) during model parameterization of metal ions was reported in the literature with a difference by dozens of kcal/mol. Here, we developed a series of nonbonded dummy models of the Mg ion targeting different HFE references in TIP3P water, followed by assessments of the designed models in the simulations of MgCl solution and biological systems. Together with the comparison of existing models, we conclude that the difference in the targeted HFEs has a limited influence on the model performance, while the usability of these models differs from case to case. The feasibility of reproducing more properties of Mg such as diffusion constants and water exchange rates using a nonbonded dummy model is demonstrated. Underestimated activity derivative and osmotic coefficient of MgCl solutions in high concentration reveal a necessity for further optimization of ion-pair interactions. The developed dummy models are applicable to metal coordination with Asp, Glu, and His residues in metalloenzymes, and the performance in predicting monodentate or bidentate binding modes of Asp/Glu residues depends on the complexity of metal centers and the choice of protein force fields. When both the binding modes coexist, the nonbonded dummy models outperform point charge models, probably in need of considering polarization of metal-binding residues by, for instance, charge calibration in classical force fields. This work is valuable for the use and further development of magnesium ion models for simulations of metal-containing systems with good accuracy.
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http://dx.doi.org/10.1021/acs.jcim.1c00281DOI Listing
June 2021

Does Helicobacter pylori infection affect the structure of bacteria in the gastric mucosa and fluid in patients with chronic antral gastritis?

J Gen Appl Microbiol 2021 May 29. Epub 2021 May 29.

Department of Gastroenterology, People's Hospital of Lishui City.

This study aimed to evaluate the composition of the gastric microbiota in the gastric mucosa and gastric fluid of patients with chronic antral gastritis. Specifically, we sought to determine whether Helicobacter pylori (Hp) infection changes the bacterial community in the gastric mucosa or alters the microbiota in the gastric fluid. The bacterial community at another site in the stomach was also investigated. DNA was extracted from 160 samples collected from 40 patients with chronic antral gastritis (20 Hp-positive and 20 Hp-negative cases). Three tissue samples of the gastric mucosa (gastric angle, body, and antral mucosa) and one tube of gastric fluid were collected from every patient. A 16S rRNA amplification library was created, and high-throughput sequencing was performed. A profile of the community composition was obtained using bioinformatics methods, including cluster, taxonomy, and diversity analyses. Analysis of the gastric bacterial community revealed that the community compositions of the gastric mucosa and gastric fluid of patients without Hp are similar to but show differences from those of Hp-positive patients. The microbiota in Hp-positive patients exhibited reduced microbial diversity, and the gastric fluid of these patients contained a small proportion of Hp. The richness of Leptotrichia in mucosal samples was greater than that in gastric fluid samples from Hp-negative patients with chronic antral gastritis. Hp changes the growth of other microbiota in the mucosa and affects the microbiota in the gastric fluid of patients with chronic antral gastritis. In addition to Hp, the presence of other bacteria might be related to the development of chronic antral gastritis.
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http://dx.doi.org/10.2323/jgam.2020.08.005DOI Listing
May 2021

The synergistic protection of EGCG and quercetin against streptozotocin (STZ)-induced NIT-1 pancreatic β cell damage via upregulation of BCL-2 expression by miR-16-5p.

J Nutr Biochem 2021 May 26:108748. Epub 2021 May 26.

College of Food Science and Engineering, Shandong Agricultural University, Key Laboratory of Food Processing Technology and Quality Control of Shandong Higher Education Institutes, Taian 271018, P.R. China. Electronic address:

EGCG and quercetin are flavonoids which usually co-exist in edible plants and they exhibit anti-diabetes effects. This study aimed to explore the mechanisms by which quercetin and EGCG synergistically protected pancreatic β-cells from streptozotocin-induced apoptosis. EGCG, quercetin, and their combinations (both 15 μM) all reversed STZ-induced cells damage and enhanced glucose-stimulated insulin secretion, with the combination being more effective than a single compound. At the molecular level, the EGCG-quercetin combination upregulated BCL-2 expression and caused a greater reduction in miR-16-5p level than EGCG alone or quercetin alone. Overexpression of miR-16-5p could offset the down-regulated apoptotic genes caused by the synergistic action of the combination. These findings suggest that EGCG and quercetin exert synergistic anti-diabetes effect, possibly via decreasing the expression of miR-16-5p that targets directly BCL-2. This is the first report on a miRNA-based mechanism underlying the synergistic protective effect of EGCG and quercetin against pancreatic cell damage.
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http://dx.doi.org/10.1016/j.jnutbio.2021.108748DOI Listing
May 2021

Effects of Aricept on intestinal flora diversity in patients with mild Alzheimer's disease explored through high-throughput sequencing technology.

Am J Transl Res 2021 15;13(4):2486-2498. Epub 2021 Apr 15.

Department of Ophtalmology, Tongde Hospital of Zhejiang Province Hangzhou, Zhejiang Province, China.

Objective: To research the effects of Aricept on the intestinal flora in patients with mild Alzheimer's disease (AD) and explore the relationship between the improvement from Aricept on AD and the changes in intestinal flora.

Methods: One month after Aricept treatment, DNA was extracted from stool samples of patients and the quality of DNA was detected. Then, the library was constructed, quantified, pooled and the quality of the library was checked. Sequencing was conducted using the Miseq sequencer and the related results were analyzed by bioinformatics.

Results: The overall structure of intestinal flora in AD patients was largely changed after Aricept treatment (P<0.05), which was mainly shown as decreased abundance of Firmicutes, Proteobacteria, actinobacteria and fusobacteria, and increased abundance of Bacteroidetes. The average abundance of intestinal flora in lipid metabolism pathwa was also different before and after treatment (P<0.05). The function of target receptor molecules in the Aricept drug target network mainly targets G-protein coupled receptors; biological processes in energy metabolism; and biological pathways mostly target proteoglycans.

Conclusion: The occurrence and progression of AD are closely related to abnormal changes in intestinal flora structure. Bile acids may improve the symptoms of mild AD by changing the intestinal flora through lipid energy metabolism. In other words, Bile acids regulate the activity of the host nervous system through intestinal flora regulation. Intestinal flora maintains the homeostasis of bile acid and further affects the physiological and pathological processes of the host. The analysis of AD related flora structure pattern helps to understand the molecular pathological basis of AD and provides theoretical basis for the development and design of innovative drugs for AD.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129215PMC
April 2021

FMR1/circCHAF1A/miR-211-5p/HOXC8 feedback loop regulates proliferation and tumorigenesis via MDM2-dependent p53 signaling in GSCs.

Oncogene 2021 May 20. Epub 2021 May 20.

Department of Neurosurgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.

Glioma is the most common and fatal primary malignant brain tumor. Glioma stem cells (GSCs) may be an important factor in glioma cell proliferation, invasion, chemoradiotherapy tolerance, and recurrence. Therefore, discovering novel GSCs related circular RNAs (circRNAs) may finds out a prospective target for the treatment of glioma. A novel circRNA-CHAF1A (circCHAF1A) was first found in our study. CircCHAF1A was overexpressed in glioma and related to the low survival rate. Functionally, it was found that no matter in vitro or in vivo, circCHAF1A can facilitate the proliferation and tumorigenesis of TP53wt GSCs. Mechanistically, circCHAF1A upregulated transcription factor HOXC8 expression in GSCs through miR-211-5p sponging. Then, HOXC8 can transcriptionally upregulate MDM2 expression and inhibited the antitumor effect of p53. Furtherly, the RNA binding protein FMR1 can bind to and promoted the expression of circCHAF1A via maintaining its stability, while HOXC8 also transcribed the FMR1 expression to form a feedback loop, which may be involved in the malignant transformation of glioma. The novel feedback loop among FMR1, circCHAF1A, miR-211-5p, and HOXC8 in GSCs can facilitate the proliferation and tumorigenesis of glioma and GSCs. It also provided a helpful biomarker for diagnosis and prognostic evaluation of glioma and may be applied to molecular targeted therapy.
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http://dx.doi.org/10.1038/s41388-021-01833-2DOI Listing
May 2021

Interface Engineering of a Sandwich Flexible Electrode [email protected] Rooted in Carbon Cloth for Enhanced Sodium-Ion Storage.

ACS Appl Mater Interfaces 2021 May 18;13(20):23794-23802. Epub 2021 May 18.

School of Materials Science and Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China.

With the growth of demand for flexible devices, the development of flexible electrodes used in energy storage devices has attracted much attention of researchers. In this work, a thin flexible cathode of Prussian blue [email protected] rooted in carbon cloth has been fabricated. The Prussian blue analogue (PBA) is an electrochemically active material grafted on flexible carbon cloth substrates, which had been precoated with polyaniline. Polyaniline as an intermediate layer can not only improve the overall electronic conductivity of the electrode but also enhance the adhesion and load of the PBAs. The electrochemical properties of the flexible cathode with a "sandwich" structure were determined in half-cells, with a superior capacity of 151 mA h·g and a striking cyclability with 96% capacity retention over 100 cycles at 100 mA h·g. This work proposes a novel perspective for the structural construction and material synthesis of flexible positive electrodes and gives new options for the practical application of flexible batteries.
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http://dx.doi.org/10.1021/acsami.1c04931DOI Listing
May 2021

Therapeutic Mechanism and Effect of Camptothecin on Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice.

J Immunol Res 2021 24;2021:5556659. Epub 2021 Apr 24.

Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China.

Camptothecin (CPT) is a cytotoxic quinoline alkaloid isolated from the bark and branches of the Chinese tree . CPT inhibits topoisomerase I. It possesses various antitumor activities and is mainly used in the treatment of colon, ovarian, liver, and bone cancers as well as leukemia. CPT inhibits the expressions of inflammatory genes and can prevent death from chronic inflammation. Therefore, we investigated the effect of CPT treatment in ulcerative colitis (UC) using DSS-induced UC mouse model; after that, we explored its potential mechanisms. Here, we found that CPT exerted protection on DSS-induced UC in rats. In addition, the administration prominently reduced the disease activity index as well as colon length of the model rats and remarkably reduced the inflammatory cytokines. Further, CPT significantly reduced several vital proinflammatory proteins in LPS-induced RAW264.7 cells. In summary, our findings demonstrate that CPT is hopefully to act as a therapeutic agent for UC.
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http://dx.doi.org/10.1155/2021/5556659DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093050PMC
April 2021

Biomimetic Hairy Whiskers for Robotic Skin Tactility.

Adv Mater 2021 May 8:e2101891. Epub 2021 May 8.

CAS Center for Excellence in Nanoscience, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, China.

Touch sensing is among the most important sensing capabilities of a human, and the same is true for smart robotics. Current research on tactile sensors is mainly concentrated on electronic skin (e-skin), but e-skin is prone to be easily dirtied, damaged, and disturbed after repeated usage, which greatly limits its practical applications in robotics. Here, by mimicking the way that animals explore the environment using hair-based sensors, a bendable biomimetic whisker mechanoreceptor (BWMR) is designed for robotic tactile sensing. Owing to the advantages of triboelectric nanogenerator technology, the BWMR can convert external mechanical stimuli into electrical signals without a power supply, which is conducive to its widespread applications in robots. Because of the leverage effect of the whisker, the BWMR can distinguish an exciting force of 1.129 μN by amplifying external weak signals, which can be further improved by increasing the whisker length. Real-time sensing is demonstrated using a BWMR, exhibiting its potential for robotic tactile systems.
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http://dx.doi.org/10.1002/adma.202101891DOI Listing
May 2021

Suppressing ion migration of CsPbBrInanocrystals by Nickel doping and the application in high-efficiency WLEDs.

Nanotechnology 2021 May 24;32(33). Epub 2021 May 24.

School of Materials Science and Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China.

All inorganic perovskite nanocrystals CsPbX(X = Cl, Br, I) are the great potential candidates for the application of high-performance light emitting diodes (LED) due to their high Photoluminescence Quantum Yield (PLQY), high defect tolerance, narrow full-width half-maximum and tunable wavelength of 410-700 nm. However, the application of red-emitting (630-650 nm) CsPbBrInanocrystals are perplexed by phase segregation due to the composition of mixed halides and the difference in halide ion mobility. Herein, we provide an effective strategy to suppressing the migration of Br/I ions through Nidoping via a facile Hot-Injection method and the PLQY was improved as well. DFT calculations show that the introduction of Nicauses a slight contraction of the host crystal structure, which improves the bond energy between Pb and halides and reduces the level of surface defects. Therefore, the phase stability is improved by Nidoping because the phase segregation caused by ion migration in the mixed phase is effectively inhibited. Meanwhile, the non-radiative recombination in the exciton transition process is reduced and the PLQY is improved. What's more, benefiting from the suppressed ion migration and enhanced PLQY, we combine the Ni-doped CsPbBrInanocrystals with different Br/I ratios and YAG: Cephosphors as color conversion layers to fabricate high efficiency WLED. When the ratio of Br/I is 9:11, WLED has a color coordinate of (0.3621, 0.3458), the color temperature of 4336 K and presents a high luminous efficiency of 113.20 lm W, color rendering index of 94.9 under the driving current of 20 mA and exhibits excellent stability, which shows great potential in the application of LED.
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http://dx.doi.org/10.1088/1361-6528/abfe91DOI Listing
May 2021

Molecular Targeted Agent and Immune Checkpoint Inhibitor Co-Loaded Thermosensitive Hydrogel for Synergistic Therapy of Rectal Cancer.

Front Pharmacol 2021 16;12:671611. Epub 2021 Apr 16.

Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.

Molecular targeted therapy has been proved effective in treatment of rectal cancer. Up-regulated expression of programmed death ligand-1 (PD-L1) was observed after the management of molecular targeted therapy, which made the therapeutic effect discounted. Tumors with higher PD-L1 expression were more sensitive and responsive to treatment of PD-L1 inhibitor. Therefore, the combination of molecular targeted therapy and immune checkpoint blockade makes sense. In this study, the copolymers of poly (ethylene glycol)-block-poly (-leucine) (PEG-PLLeu) were synthesized as a thermosensitive hydrogel composite for consecutive release of regorafenib (REG) and BMS202. The mechanical properties of PEG-PLLeu were investigated, confirming that PEG-PLLeu (5 wt.%) was suitable for injection as drug-delivery composite at low temperature and stable after sol-gel transition at body temperature. Importantly, the double drug loaded hydrogel showed superior antitumour activity over single drugs in an orthotopic rectal cancer model (CT26-Luc). Further analysis of the tumor tissues suggested that REG upregulated the expression of PD-L1 in tumor tissues. In addition, the immunosuppressive tumor microenvironment of CT26-Luc tumor was distinctly relieved under the effect of BMS202, as characterized by increased infiltration of CD8 T cells in tumors and enhanced secretion of antitumour cytokines (IFN-γ and TNF-α). Moreover, the drug-loaded composite showed no obvious toxicity in histological analysis. Taken together, the administration of REG and BMS202 in the PEG-PLLeu composite could induce a synergistic effect in treatment of rectal cancer without obvious toxicity, and thus represented a potential strategy for enhanced therapeutic modality.
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http://dx.doi.org/10.3389/fphar.2021.671611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085774PMC
April 2021

Dual-frequency Doppler velocimeter based on delay interferometric optical phase-locking.

Opt Lett 2021 May;46(9):2103-2106

We present a dual-frequency laser Doppler velocimeter (DF-LDV) relying on a DF laser source (DFLS) generated by optical phase-locking two individual lasers to a common unbalanced Mach-Zehnder interferometer, which allows achieving high stability regardless of the DF separation of the lasers. This DFLS is evaluated using an optical frequency comb, testifying to the generation of DFLS with large DF separation up to terahertz with flexible tunability and high stability. Demonstration of DF-LDV using the DFLS of ${\sim}1.024\; {\rm THz}$ separation has achieved $1.62 \times {10^{- 2}}$ mm/s velocity resolution even for a slow velocity of $1.8\; {\rm mm}/{\rm s}$ in a mere 5 s acquisition time, confirming the high resolution and efficient speckle noise suppression enabled by the proposed DF-LDV. Featuring high precision, flexibility, and robustness, this method is particularly attractive from the practical point of view.
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http://dx.doi.org/10.1364/OL.420402DOI Listing
May 2021

Transcriptome-Wide Analysis for Ginsenoside Rb3 Synthesis-Related Genes and Study on the Expression of Methyl Jasmonate Treatment in .

Life (Basel) 2021 Apr 25;11(5). Epub 2021 Apr 25.

College of Life Science, Jilin Agricultural University, Changchun 130118, China.

C. A. Meyer is a kind of renascent herb that belongs to the genus in the family . It is a traditional Chinese precious herbal medicine with a long history of medicinal use. Ginsenoside Rb3 is one of the important active ingredients in ginseng and has important physiological activity in the treatment of many diseases. In this study, we screened and systematically analyzed the candidate genes related to ginsenoside Rb3 synthesis through bioinformatics methods; discussed the functions, expression patterns, and interactions of the genes related to ginsenoside Rb3 synthesis; and finally, selected seven genes, mainly that directly contribute to the synthesis of ginsenoside Rb3. This study provides a reference for revealing the expression rules of ginsenoside Rb3 synthesis-related genes and elucidating the regulatory mechanism of methyl jasmonate, lays a theoretical foundation for the research of ginsenoside Rb3 synthesis, and provides theoretical and technical support for the factory production of ginsenoside monomer saponins.
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http://dx.doi.org/10.3390/life11050387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146951PMC
April 2021

Paternal Jail Incarceration and Birth Outcomes: Evidence from New York City, 2010-2016.

Matern Child Health J 2021 Apr 29. Epub 2021 Apr 29.

Department of Sociology, Duke University, Durham, NC, United States.

Objectives: To examine population-level associations between paternal jail incarceration during pregnancy and infant birth outcomes using objective measures of health and incarceration.

Methods: We use multivariate logistic regression models and linked records on all births and jail incarcerations in New York City between 2010 and 2016.

Results: 0.8% of live births were exposed to paternal incarceration during pregnancy or at the time of birth. After accounting for parental sociodemographic characteristics, maternal health behaviors, and maternal health care access, paternal incarceration during pregnancy remains associated with late preterm birth (OR = 1.34, 95% CI = 1.21, 1.48), low birthweight (OR = 1.39, 95% CI = 1.27, 1.53), small size for gestational age (OR = 1.35, 95% CI = 1.17, 1.57), and NICU admission (OR = 1.14, 95% CI = 1.05, 1.24).

Conclusions: We found strong positive baseline associations (p < 0.001) between paternal jail incarceration during pregnancy with probabilities of all adverse outcomes examined. These associations did not appear to be driven purely by duration or frequency of paternal incarceration. These associations were partially explained by parental characteristics, maternal health behavior, and health care. These results indicate the need to consider paternal incarceration as a potential stressor and source of trauma for pregnant women and infants.
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http://dx.doi.org/10.1007/s10995-021-03168-6DOI Listing
April 2021

Lithium ion battery degradation: what you need to know.

Phys Chem Chem Phys 2021 Apr 26;23(14):8200-8221. Epub 2021 Mar 26.

Department of Mechanical Engineering, Imperial College London, London SW7 2AZ, UK.

The expansion of lithium-ion batteries from consumer electronics to larger-scale transport and energy storage applications has made understanding the many mechanisms responsible for battery degradation increasingly important. The literature in this complex topic has grown considerably; this perspective aims to distil current knowledge into a succinct form, as a reference and a guide to understanding battery degradation. Unlike other reviews, this work emphasises the coupling between the different mechanisms and the different physical and chemical approaches used to trigger, identify and monitor various mechanisms, as well as the various computational models that attempt to simulate these interactions. Degradation is separated into three levels: the actual mechanisms themselves, the observable consequences at cell level called modes and the operational effects such as capacity or power fade. Five principal and thirteen secondary mechanisms were found that are generally considered to be the cause of degradation during normal operation, which all give rise to five observable modes. A flowchart illustrates the different feedback loops that couple the various forms of degradation, whilst a table is presented to highlight the experimental conditions that are most likely to trigger specific degradation mechanisms. Together, they provide a powerful guide to designing experiments or models for investigating battery degradation.
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http://dx.doi.org/10.1039/d1cp00359cDOI Listing
April 2021

Three-Dimensional Network Pd-Ni/γ-AlO Catalysts for Highly Active Catalytic Hydrogenation of Nitrobenzene to Aniline under Mild Conditions.

ACS Omega 2021 Apr 1;6(14):9780-9790. Epub 2021 Apr 1.

Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, 438West Hebei Street, Qinhuangdao 066004, China.

In view of the current situation of high cost and low catalytic efficiency of the commercial Pd-based catalysts, adding transition metals (Ni, Co, etc.) to form the Pd-M bimetallic catalyst not only reduces the consumption of Pd but also greatly improves the catalytic activity and stability, which has attracted increasing attention. In this work, the three-dimensional network Pd-Ni bimetallic catalysts were prepared successfully by a liquid-phase in situ reduction method with the hydroxylated γ-AlO as the support. Through investigating the effects of the precursor salt amount, reducing agent concentration, stabilizer concentration, and reducing stirring time on the synthesis of the Pd-Ni nanocatalyst, the three-dimensional network Pd-Ni bimetallic nanostructures with four different atomic ratios were prepared under an optimal condition. The obtained wire-like Pd-Ni catalysts have a uniform diameter size of about 5 nm and length up to several microns. After closely combining with the hydroxylated γ-AlO, the supported Pd-Ni/γ-AlO catalysts exhibit nearly 100% conversion rate and selectivity for the hydrogenation of nitrobenzene to aniline at low temperature and normal pressure. The stability testing of the supported Pd-Ni/γ-AlO catalysts shows that the conversion rate still remained above 99% after 10 cycles. There is no doubt that the supported catalysts show significant catalytic efficiency and recyclability, which provides important theoretical basis and technical support for the preparation of low-cost, highly efficient catalysts for the hydrogenation of nitrobenzene to aniline.
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http://dx.doi.org/10.1021/acsomega.1c00441DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047756PMC
April 2021

Measures of resting state EEG rhythms for clinical trials in Alzheimer's disease: Recommendations of an expert panel.

Alzheimers Dement 2021 Apr 15. Epub 2021 Apr 15.

School of Psychology, University of Glasgow, Glasgow, UK.

The Electrophysiology Professional Interest Area (EPIA) and Global Brain Consortium endorsed recommendations on candidate electroencephalography (EEG) measures for Alzheimer's disease (AD) clinical trials. The Panel reviewed the field literature. As most consistent findings, AD patients with mild cognitive impairment and dementia showed abnormalities in peak frequency, power, and "interrelatedness" at posterior alpha (8-12 Hz) and widespread delta (< 4 Hz) and theta (4-8 Hz) rhythms in relation to disease progression and interventions. The following consensus statements were subscribed: (1) Standardization of instructions to patients, resting state EEG (rsEEG) recording methods, and selection of artifact-free rsEEG periods are needed; (2) power density and "interrelatedness" rsEEG measures (e.g., directed transfer function, phase lag index, linear lagged connectivity, etc.) at delta, theta, and alpha frequency bands may be use for stratification of AD patients and monitoring of disease progression and intervention; and (3) international multisectoral initiatives are mandatory for regulatory purposes.
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http://dx.doi.org/10.1002/alz.12311DOI Listing
April 2021

The SRSF1/circATP5B/miR-185-5p/HOXB5 feedback loop regulates the proliferation of glioma stem cells via the IL6-mediated JAK2/STAT3 signaling pathway.

J Exp Clin Cancer Res 2021 Apr 15;40(1):134. Epub 2021 Apr 15.

Department of Neurosurgery, the First Hospital of China Medical University, NO.155, North Nanjing Street, Heping District, Shenyang City, 110001, China.

Background: Glioma is the most common and malignant tumor of central nervous system. The tumor initiation, self-renewal, and multi-lineage differentiation abilities of glioma stem cells (GSCs) are responsible for glioma proliferation and recurrence. Although circular RNAs (circRNAs) play vital roles in the progression of glioma, the detailed mechanisms remain unknown.

Methods: qRT-PCR, western blotting, immunohistochemistry, and bioinformatic analysis were performed to detect the expression of circATP5B, miR-185-5p, HOXB5, and SRSF1. Patient-derived GSCs were established, and MTS, EDU, neurosphere formation, and limiting dilution assays were used to detect the proliferation of GSCs. RNA-binding protein immunoprecipitation, RNA pull-down, luciferase reporter assays, and chromatin immunoprecipitation assays were used to detect these molecules' regulation mechanisms.

Results: We found circATP5B expression was significantly upregulated in GSCs and promoted the proliferation of GSCs. Mechanistically, circATP5B acted as miR-185-5p sponge to upregulate HOXB5 expression. HOXB5 was overexpressed in glioma and transcriptionally regulated IL6 expression and promoted the proliferation of GSCs via JAK2/STAT3 signaling. Moreover, RNA binding protein SRSF1 could bind to and promote circATP5B expression and regulate the proliferation of GSCs, while HOXB5 also transcriptionally regulated SRSF1 expression.

Conclusions: Our study identified the SRSF1/circATP5B/miR-185-5p/HOXB5 feedback loop in GSCs. This provides an effective biomarker for glioma diagnosis and prognostic evaluation.
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http://dx.doi.org/10.1186/s13046-021-01931-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051130PMC
April 2021

Sec62 promotes stemness and chemoresistance of human colorectal cancer through activating Wnt/β-catenin pathway.

J Exp Clin Cancer Res 2021 Apr 15;40(1):132. Epub 2021 Apr 15.

Hepatopancreatobiliary Surgery Department I, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital & Institute, Beijing, 100142, China.

Background: Cancer stem cell (CSC)-related chemoresistance leads to poor outcome of the patients with colorectal cancer (CRC). In this study, we identified the chemoresistance-relevant molecules and decipher the involved mechanisms to provide potential therapeutic target for CRC. We focused on Sec62, a novel target with significantly increased expression in chemoresistant CRC tissues, and further investigated its role in the progression of CRC.

Methods: Through analyzing the differentially-expressed genes between chemoresistant and chemosensitive CRCs, we selected Sec62 as a novel chemoresistance-related target in CRC. The expression and clinical significance of Sec62 were determined by immunoblotting and immunohistochemistry in tissues and cell lines of CRC. The roles of Sec62 in drug resistance, stemness and tumorigenesis were evaluated in vitro and in vivo using functional experiments. GST pull-down, western blot, coimmunoprecipitation and Me-RIP assays were performed to further explore the downstream molecular mechanisms.

Results: Sec62 upregulation was associated with the chemoresistance of CRC and poor outcome of CRC patients. Depletion of Sec62 sensitized CRC cells to chemotherapeutic drugs. Sec62 promoted the stemness of CRC cells through activating Wnt/β-catenin signaling. Mechanistically, Sec62 bound to β-catenin and inhibited the degradation of β-catenin. Sec62 competitively disrupted the interaction between β-catenin and APC to inhibit the β-catenin destruction complex assembly. Moreover, Sec62 expression was upregulated by the mA-mediated stabilization of Sec62 mRNA.

Conclusions: Sec62 upregulated by the METTL3-mediated mA modification promotes the stemness and chemoresistance of CRC by binding to β-catenin and enhancing Wnt signalling. Thus, mA modification-Sec62-β-catenin molecular axis might act as therapeutic targets in improving treatment of CRC.
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http://dx.doi.org/10.1186/s13046-021-01934-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051072PMC
April 2021

Robotic and laparoscopic sacrocolpopexy for pelvic organ prolapse: a systematic review and meta-analysis.

Ann Transl Med 2021 Mar;9(6):449

Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, China.

Background: Sacrocolpopexy is the gold standard procedure for treating pelvic organ prolapse (POP) patients with apical defects. Different surgical approaches have emerged and been utilized successively, including traditional laparoscopy, single-hole laparoscopy, robotic laparoscopy, vaginal-assisted laparoscopy, and transvaginal approaches. Robotic sacrocolpopexy (RSC) has attracted increasing attention as an emerging surgical technique and has unique advantages, such as a "simulated wrist" mechanical arm and high-definition three-dimensional (3D) visual field, which has gradually begun to be utilized in the clinical setting.

Methods: We followed the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) reporting checklist, and a systematic literature search was conducted on six databases from their inception to 1st March 2020. We evaluated patients with POP who underwent RSC or laparoscopic sacrocolpopexy (LSC), outcomes (including perioperative outcomes: blood loss, operating times, blood transfusion, and hospital stay), surgery-related complications, as well as cure and recurrence rates.

Results: A total of 49 articles were available, including 3,014 patients, among which 18 were comparative studies on LSC RSC, and 31 were non-comparative single-arm studies on RSC. For RSC, median operative time was 226 [90-604] minutes, estimated blood loss was 56 [5-1,500] mL, and hospital stay was 1.55 [1-16] days. Intraoperative complications and postoperative complications occurred in 74 (2.7%) and 360 (13.0%) patients, respectively. Of 2,768 RSC patients, 40 had been converted from a robot-assisted approach to other approaches, and 134 of 1,852 patients (7.2%) have recurrent prolapses of any compartment. Compared to LSC, RSC was associated with significantly lower blood loss and lower conversion rate. However, more operative time was observed in RSC. No significant differences were observed in perioperative transfusion, intraoperative and postoperative complications, or objective recurrence between RSC and LSC.

Conclusions: RSC's application seems to contribute some advantages compared to conventional laparoscopic surgery, although both approaches appear to promote equivalent clinical outcomes. Notably, heterogeneity among studies might have affected the outcome of the study. Consequently, high-quality and large-sample randomized trials comparing both techniques are necessitated.
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http://dx.doi.org/10.21037/atm-20-4347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039662PMC
March 2021

Paracrine interleukin-8 affects mesenchymal stem cells through the Akt pathway and enhances human umbilical vein endothelial cell proliferation and migration.

Biosci Rep 2021 May;41(5)

Department of Anatomy, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, P.R. China.

Interleukin-8 (IL-8) promotes cell homing and angiogenesis, but its effects on activating human bone marrow mesenchymal stem cells (BMSCs) and promoting angiogenesis are unclear. We used bioinformatics to predict these processes. In vitro, BMSCs were stimulated in a high-glucose (HG) environment with 50 or 100 μg/ml IL-8 was used as the IL-8 group. A total of 5 μmol/l Triciribine was added to the two IL-8 groups as the Akt inhibitor group. Cultured human umbilical vein endothelial cells (HUVECs) were cultured in BMSCs conditioned medium (CM). The changes in proliferation, apoptosis, migration ability and levels of VEGF and IL-6 in HUVECs were observed in each group. Seventy processes and 26 pathways were involved in vascular development, through which IL-8 affected BMSCs. Compared with the HG control group, HUVEC proliferation absorbance value (A value), Gap closure rate, and Transwell cell migration rate in the IL-8 50 and IL-8 100 CM groups were significantly increased (P<0.01, n=30). However, HUVEC apoptosis was significantly decreased (P<0.01, n=30). Akt and phospho-Akt (P-Akt) protein contents in lysates of BMSCs treated with IL-8, as well as VEGF and IL-6 protein contents in the supernatant of BMSCs treated with IL-8, were all highly expressed (P<0.01, n=15). These analyses confirmed that IL-8 promoted the expression of 41 core proteins in BMSCs through the PI3K Akt pathway, which could promote the proliferation and migration of vascular endothelial cells. Therefore, in an HG environment, IL-8 activated the Akt signaling pathway, promoted paracrine mechanisms of BMSCs, and improved the proliferation and migration of HUVECs.
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http://dx.doi.org/10.1042/BSR20210198DOI Listing
May 2021

Magnetic field measurement based on a fiber laser oscillation circuit merged with a polarization-maintaining fiber Sagnac interference structure.

Opt Express 2021 Mar;29(6):8763-8769

A compact optical magnetic field sensor based on a fiber laser oscillator circuit merged with a Sagnac loop that contains a polarization-maintaining fiber (PMF) and a magnetostrictive rod is proposed. The combination of the PMF-Sagnac loop and fiber laser oscillation circuit significantly increases the signal-to-noise ratio of the reflection spectrum in the system, which is a mixed spectrum that includes interference fringes and lasers. For the proposed system, an erbium-doped fiber amplifier is used to provide gain, a dispersion compensating fiber is used to compensate for dispersion in the system, a PMF is inserted in the Sagnac loop as a microwave filter, while a section of the PMF is bonded to a magnetostrictive rod to achieve magnetic field sensing. The proposed system is shown experimentally to produce a fine reflection interference spectrum. The highest intensity reflection interference peak (from the laser) is over 40 dB with a -3 dB line-width of about 0.03 nm. The system can provide magnetic field sensitivities of 0.07 nm/mT and 0.076 nm/mT for sensor head lengths of 1 m and 2 m, respectively, and exhibits a stability of ±0.029 nm over 1 h. The proposed sensing system has advantages of low cost, high sensitivity, compact structure, and can produce a fine and stable reflection spectrum.
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http://dx.doi.org/10.1364/OE.419306DOI Listing
March 2021

A Usability Study of Low-cost Wireless Brain-Computer Interface for Cursor Control Using Online Linear Model.

IEEE Trans Hum Mach Syst 2020 Aug 14;50(4):287-297. Epub 2020 May 14.

Department of Mechanical, Aerospace, and Biomedical Engineering, University of Tennessee, Knoxville, TN 37996 USA.

Computer cursor control using electroencephalogram (EEG) signals is a common and well-studied brain-computer interface (BCI). The emphasis of the literature has been primarily on evaluation of the objective measures of assistive BCIs such as accuracy of the neural decoder whereas the subjective measures such as user's satisfaction play an essential role for the overall success of a BCI. As far as we know, the BCI literature lacks a comprehensive evaluation of the usability of the mind-controlled computer cursor in terms of decoder efficiency (accuracy), user experience, and relevant confounding variables concerning the platform for the public use. To fill this gap, we conducted a two-dimensional EEG-based cursor control experiment among 28 healthy participants. The computer cursor velocity was controlled by the imagery of hand movement using a paradigm presented in the literature named imagined body kinematics (IBK) with a low-cost wireless EEG headset. We evaluated the usability of the platform for different objective and subjective measures while we investigated the extent to which the training phase may influence the ultimate BCI outcome. We conducted pre- and post- BCI experiment interview questionnaires to evaluate the usability. Analyzing the questionnaires and the testing phase outcome shows a positive correlation between the individuals' ability of visualization and their level of mental controllability of the cursor. Despite individual differences, analyzing training data shows the significance of electrooculogram (EOG) on the predictability of the linear model. The results of this work may provide useful insights towards designing a personalized user-centered assistive BCI.
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http://dx.doi.org/10.1109/thms.2020.2983848DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990128PMC
August 2020

Chemotherapy-induced amenorrhea and its effects on fertility in long-term female survivors of classic osteosarcoma.

Support Care Cancer 2021 Mar 26. Epub 2021 Mar 26.

Bone Oncology, Beijing Jishuitan Hospital, 31 east street of Xinjiekou, Xicheng District, Beijing, China.

Purpose: To explore the effect of chemotherapy-induced amenorrhea (CIA) on female osteosarcoma patients' fertility function, we investigated and analyzed their marital status, fertility, and menstrual status in a retrospective cohort study.

Methods: We selected female osteosarcoma patients from database from January 2004 to December 2013. Patients' characteristics such as age, tumor location, marital status, menstrual status, and fertility status were collected. The data were analyzed using the Statistical Package for the Social Sciences (SPSS), version 22.

Results: A total of 122 female patients met these criteria and finally responded by questionnaire and telephone follow-up. The marriage rate of female osteosarcoma survivors was 50.8% (62/122), which was significantly lower than the control group (p = 0.000). The average marriage age of female osteosarcoma survivors was 25.5, which was obviously higher than the control group (p = 0.000). CIA occurred in 46 (36.1%) patients. We then found that the incidence of CIA was higher in older patients. (p = 0.011). All of the married patients wanted to have children, and 67.8% (42/62) of them had given birth after chemotherapy. The fertility of married patients with CIA was significantly reduced compared to that of married patients without CIA. (p = 0.001).

Conclusions: The patients with CIA have higher risk of impaired reproductive function than those who did not. Fertility preservation option before the start of the chemotherapy is important. And it is much value to record menstrual pattern and detect sex steroid levels after 6 months of therapy in order to be able to evaluate the fertility status.
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http://dx.doi.org/10.1007/s00520-021-06069-0DOI Listing
March 2021

[Astragaloside II inhibits the proliferation of rat pulmonary artery smooth muscle cells induced by hypoxia via blocking NOX/ROS/AKT/mTOR signaling pathway].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Mar;37(3):219-224

Basic Discipline of Integrated Chinese and Western Medicine, Heilongjiang University of Chinese Medicine, Harbin 150000, China. *Corresponding authors, E-mail:

Objective To investigate the inhibitory effect of astragaloside II (AS-II) on the proliferation of pulmonary artery smooth muscle cells (PASMCs) induced by hypoxia and its relevant mechanism. Methods Rat primary PASMCs were divided into normoxia group, hypoxia group, hypoxia combined with 20, 40, 80 μmol/L AS-II treated groups, hypoxia combined with nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) inhibitor VAS2870 treated group, and then cultured either in normoxic (210 mL/L O) or hypoxic (20 mL/L O) condition for 24 hours. The proliferation of PASMCs was detected by CCK-8 assay. The level of intracellular reactive oxygen species (ROS) was detected by DCFH-DA staining. Protein kinase B (AKT), phospho-AKT (p-AKT), mammalian target of rapamycin (mTOR), phospho-mTOR (p-mTOR), proliferating cell nuclear antigen (PCNA), NOX1 and NOX4 protein expression were assessed by Western blotting. Results In the hypoxia group, the proliferation of PASMCs, level of intracellular ROS, protein expression of PCNA, p-AKT, p-mTOR, NOX1 and NOX4 increased significantly compared with those in the normoxia group. However, AS-II treatment inhibited hypoxia-induced PASMCs proliferation, decreased the level of intracellular ROS, and suppressed protein expression of PCNA, p-AKT, p-mTOR, NOX1 and NOX4. Moreover, VAS2870 treatment lead to similar changes. Conclusion AS-II can inhibit the proliferation of PASMCs induced by hypoxia, which may be associated with the blocking of NOX/ROS/AKT/mTOR signaling pathway.
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March 2021

Pan-Cancer Analysis of NOS3 Identifies Its Expression and Clinical Relevance in Gastric Cancer.

Front Oncol 2021 4;11:592761. Epub 2021 Mar 4.

The First Laboratory of Cancer Institute, The First Hospital of China Medical University, Shenyang, China.

(endothelial NOS, ) is a member of the nitric oxide synthase (NOS) enzyme family, mainly participating in nitric oxide (NO) generation. has been reported to inhibit apoptosis and promote angiogenesis, proliferation, and invasiveness. However, the expression pattern of and its diagnostic and prognostic potential has not been investigated in a pan-cancer perspective. Data from the Genotype-Tissue Expression (GTEx), the Cancer Genome Atlas (TCGA), the Cancer Cell Line Encyclopedia (CCLE), and the Cancer Therapeutics Response Portal (CTRP) were employed and expression was comprehensively analyzed in normal tissues, cancer tissues, and cell lines. Immunohistochemical staining of tissue sections were used to validate the prognostic role of in gastric cancer patients. GSVA and GSEA analyses were performed to investigate signaling pathways related to expression. In normal tissues, was expressed highest in the spleen and lowest in the blood. expression was increased in stomach adenocarcinoma (STAD) and significantly associated with poor prognosis of patients. Immunohistochemical staining validated that was an independent prognostic factor of gastric cancer. Several canonical cancer-related pathways were found to be correlated with expression in STAD. The expression of NOS3 was related to the response to QS-11 and brivinib in STAD. was an independent prognostic factor for patients with STAD. Increased expression of influenced occurrence and development of STAD through several canonical cancer-related pathways. Drug response analysis reported drugs to suppress NOS3. NOS3 might be a novel target for gastric cancer treatment.
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http://dx.doi.org/10.3389/fonc.2021.592761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969995PMC
March 2021

Silencing of PGK1 Promotes Sensitivity to Paclitaxel Treatment by Upregulating XAF1-Mediated Apoptosis in Triple-Negative Breast Cancer.

Front Oncol 2021 4;11:535230. Epub 2021 Mar 4.

Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, China.

Triple negative breast cancer (TNBC) is known to have aggressive clinical course and a high risk of recurrence. Given the lack of effective targeted therapy options, paclitaxel-based chemotherapy is still the primary option for TNBC patients. However, patients who fail to achieve a complete response during neoadjuvant chemotherapy may be mainly due to sensitivity and resistance to chemotherapy. Thus, we concentrated the present research on the role of PGK1 in the sensitivity to paclitaxel treatment and the possible underlying mechanisms in TNBC. After exposure to paclitaxel, a cell viability analysis was made to investigate the influence of PGK1 silencing on cell death. The effect of PGK1 on apoptosis induced by paclitaxel treatment was examined by flow cytometry cell apoptosis assays. Western blotting was performed to examine the impact of PGK1 on paclitaxel-induced apoptosis. The correlation of PGK1 with apoptosis-associated protein X-linked inhibitor of apoptosis (XIAP)-associated factor 1 (XAF1) was analyzed in 39 specimens by immunohistochemistry analysis. We observed that silencing PGK1 sensitized triple-negative breast cancer (TNBC) cell lines to paclitaxel treatment as a result of increased drug-induced apoptosis. Furthermore, mechanistic investigations suggested that XAF1 was increased in PGK1-knockdown cells along with the expression of the apoptotic proteins including cleaved caspase-3 and Bax. Immunohistochemistry analysis showed that PGK1 was negatively related to XAF1. Moreover, we found that downregulation of XAF1 reduced paclitaxel-induced apoptosis in PGK1-silenced triple-negative cell lines. Our results identified PGK1 as a potential biomarker for the treatment of TNBC, and inhibition of PGK1 expression might represent a novel strategy to sensitize TNBC to paclitaxel treatment.
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http://dx.doi.org/10.3389/fonc.2021.535230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969978PMC
March 2021

Determination of seven tetracyclines in milk by dissolvable layered double hydroxide-based solid-phase extraction coupled with high-performance liquid chromatography.

Anal Methods 2021 04 18;13(13):1618-1624. Epub 2021 Mar 18.

School of Public Health, Southwest Medical University, Luzhou 646000, China.

A dissolvable layered double hydroxide-based solid-phase extraction combined with high-performance liquid chromatography was developed for the analysis of minocycline, oxytetracycline, tetracycline, demeclocycline, metacycline, chlortetracycline and doxycycline in milk samples. In situ formation of the layered double hydroxide was achieved by the addition of MgCl-AlCl solution to alkaline deproteinized milk. The analytes were efficiently extracted by the Mg/Al layered double hydroxide. After centrifugation, the co-precipitates were dissolved in 0.1 mol L NaEDTA-McIlvaine buffer prior to HPLC analysis. Under optimized conditions, the method achieved low detection limits of 0.414-0.986 μg L and quantification limits of 1.38-3.29 μg L, and good recoveries of 93.5-100% with intra- and inter-day RSDs of 0.498-4.08% and 1.23-10.0%, respectively. This method is convenient, accurate, sensitive, rapid, cost-effective, eco-friendly, and suitable for the determination of seven tetracycline antibiotics in milk samples.
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http://dx.doi.org/10.1039/d1ay00154jDOI Listing
April 2021