Publications by authors named "Yang Deng"

253 Publications

Large-scale analysis of 2,152 Ig-seq datasets reveals key features of B cell biology and the antibody repertoire.

Cell Rep 2021 May;35(6):109110

Department of Epidemiology, Second Military Medical University, 800 Xiangyin Rd., Shanghai 200433, China. Electronic address:

Antibody repertoire sequencing enables researchers to acquire millions of B cell receptors and investigate these molecules at the single-nucleotide level. This power and resolution in studying humoral responses have led to its wide applications. However, most of these studies were conducted with a limited number of samples. Given the extraordinary diversity, assessment of these key features with a large sample set is demanded. Thus, we collect and systematically analyze 2,152 high-quality heavy-chain antibody repertoires. Our study reveals that 52 core variable genes universally contribute to more than 99% of each individual's repertoire; a distal interspersed preferences characterize V gene recombination; the number of public clones between two repertoires follows a linear model, and the positive selection dominates at RGYW motif in somatic hypermutations. Thus, this population-level analysis resolves some critical features of the antibody repertoire and may have significant value to the large cadre of scientists.
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http://dx.doi.org/10.1016/j.celrep.2021.109110DOI Listing
May 2021

Making Waves: Principles for the Design of Sustainable Household Water Treatment.

Authors:
Yang Deng

Water Res 2021 Apr 13;198:117151. Epub 2021 Apr 13.

Department of Earth and Environmental Studies, Montclair State University, Montclair, New Jersey 07043, United States. Electronic address:

Household water treatment (HWT) represents a key engineering intervention for billions of people's daily and emergency water needs. However, established HWT practices experience different challenges to achieve sustainability. Here, five essential principles are identified and discussed for the design of sustainable HWT, including treatment capability, environmental friendliness, user experience, economic viability, and social acceptance. A well-implemented HWT, with a consideration of energy resilience, relies heavily on advanced materials, innovative technologies, and/or creative designs capable of reliably abating both traditional and emerging contaminants, while minimizing undesirable chemical leaching and the formation of harmful disinfection byproducts. Environmental friendliness can be pursued by appreciably reducing environmental and energy footprints and properly managing HWT residuals. Meanwhile, a user-centered design approach and economic consideration need to be integrated into the HWT development to improve end users' willingness to use and pay, respectively. Finally, social acceptance should be intertwined by fostering public acceptance and market adoption for strengthening commercial viability of new-generation HWT products. Given that these principles span across different domains, multidisciplinary collaboration is required throughout different stages of the HWT technology innovation.
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http://dx.doi.org/10.1016/j.watres.2021.117151DOI Listing
April 2021

HIV incidence and sexual behavioral correlates among 4578 men who have sex with men (MSM) in Chengdu, China: a retrospective cohort study.

BMC Public Health 2021 Apr 26;21(1):802. Epub 2021 Apr 26.

School of Public Health, Sun Yat-sen University (North Campus), No.74, Zhongshan second road, Guangzhou, China.

Background: The prevalence of HIV among men who have sex with men (MSM) in southwest China is still increasing. This study aimed to investigate the trend in HIV incidence and its associated risk factors among MSM in Chengdu, China.

Method: Incidence data were collected from the largest local non-governmental organization (NGO) serving MSM in Chengdu between 2012 and 2018, while information on sexual behaviors was collected from 2014. All MSM who received voluntary counseling and testing services (VCT) in the collaborating NGO at least twice during the study period were included. We calculated the HIV incidence density among MSM every 2 years and the overall incidence rate. A Cox proportional hazards regression model was employed to identify risk factors for HIV infection.

Result: A total of 4578 HIV-negative participants were included in the cohort. The total incidence density was 5.95 (95% CI: 5.37-6.56)/100 person-years (PYs) between 2012 and 2018. The segmented incidence density was 9.02 (95% CI: 7.46-10.78), 5.85 (95% CI: 4.86-6.97), 5.43 (95% CI: 4.53-6.46), and 3.09 (95% CI: 2.07-4.41)/100 PYs in 2012-2013, 2014-2015, 2016-2017, and 2018, respectively. After adjusting for sociodemographic characteristics, compared to participants without sexual partners within 6 months, MSM with one fixed partner (Adjusted Hazard Ratio, AHR = 1.18, 95% CI: 0.44-3.19) and more than five partners (AHR = 2.24, 95% CI: 0.81-6.20) had increased risk of HIV infection. MSM who used condom inconsistently had a higher risk of HIV infection (AHR = 1.87, 95% CI: 1.46-2.38) compared to consistent condom users.

Conclusion: The decreased HIV incidence density among MSM was potentially related to the successful comprehensive HIV prevention strategies in Chengdu. Multiple male sexual partnerships and inconsistent condom use during anal intercourse were risk factors associated with HIV occurrence.
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http://dx.doi.org/10.1186/s12889-021-10835-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077884PMC
April 2021

Lipopolysaccharide stimulates bovine endometrium explants through toll‑like receptor 4 signaling and PGE synthesis.

Prostaglandins Leukot Essent Fatty Acids 2021 May 24;168:102272. Epub 2021 Mar 24.

Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, 010018, Hohhot, China. Electronic address:

Bovine endometrium infection with gram-negative bacteria commonly causes uterine diseases. Previous studies indicate that prostaglandin E (PGE) is an inflammatory mediator in bacterial endometritis. However, the mechanism underlying lipopolysaccharide (LPS)-induced inflammatory response regulation in bovine endometrial explants remains elusive. In the present study, bovine explants were pre-treated with 15-hydroxyprostaglandin dehydrogenase (15-PGDH) inhibitors before LPS stimulation. PGE secretion, prostaglandin synthetase, pro-inflammatory factor, damage-associated molecular pattern (DAMP), and related signaling pathway factor levels were evaluated. Using 15-PGDH inhibitors pre-treatment, LPS-treated bovine endometrial explants exhibited augmentation of PGE and DAMP expression, and upregulation of various signaling pathway factors. Protein kinase A (PKA), extracellular-signal-regulated kinase, and c-Jun N-terminal kinase phosphorylation and degradation of nuclear transcription factor-κB (NF-κB) inhibitors were induced in the pre-treated endometrial explants. The mechanism underlying LPS-induced PGE accumulation acting as a pro-inflammatory mediator through toll-like receptor 4 signaling in bovine explants could involve the PKA, mitogen-activated protein kinase, and NF-κB pathways.
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http://dx.doi.org/10.1016/j.plefa.2021.102272DOI Listing
May 2021

Association Between High-Sensitivity Troponin T on Admission and Organ Dysfunction During Hospitalization in Patients Aged 80 Years and Older with Hip Fracture: A Single-Centered Prospective Cohort Study.

Clin Interv Aging 2021 6;16:583-591. Epub 2021 Apr 6.

Department of Geriatric Orthopedics, Sichuan Provincial Orthopedic Hospital, Chengdu, Sichuan, People's Republic of China.

Background: Prognostic evaluation of elderly patients with hip fracture is an issue that has been highly concerned by clinicians. Only a few studies have focused on organ dysfunction after hip fracture in the elderly. This study aimed to investigate the association between high-sensitivity troponin T (hs-TnT) at admission and organ dysfunction during hospitalization in elderly patients with hip fracture.

Methods: We enrolled 168 patients with hip fracture who were aged 80 years and older at Geriatric Orthopaedic Center of Sichuan Provincial Orthopedic Hospital between January 2020 and August 2020. Baseline characteristics, perioperative information, and short-term clinical outcomes were analyzed.

Results: Of the 208 patients admitted during the study period, 168 met the inclusion criteria; of these, 91 (54.2%) had higher hs-TnT than the 99th percentile in the normal population. After adjustment for confounders, elevated hs-TnT was independently associated with multiple organ dysfunction syndrome in the elderly (MODSE) (adjusted OR, 5.76; 95% CI, 1.74-19.10; = 0.004), heart dysfunction (adjusted OR, 7.48; 95% CI, 2.17-25.82; = 0.001), MODS severity score > 3 (adjusted OR, 5.22; 95% CI, 1.32-20.60; = 0.018), and length of hospital stay > 14 days (adjusted OR, 2.38; 95% CI, 1.05-5.36; = 0.037).

Conclusion: Increased hs-TnT on admission is an independent risk factor for MODSE after hip fracture in patients aged 80 years and older. Effective measures should be applied to avoid progression of MODSE from pre-failure stage to failure stage.
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http://dx.doi.org/10.2147/CIA.S303246DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039433PMC
April 2021

Years of Life Lost Due to Premature Death and Their Trends in People With Selected Neurological Disorders in Shanghai, China, 1995-2018: A Population-Based Study.

Front Neurol 2021 5;12:625042. Epub 2021 Mar 5.

Center for Disease Control and Prevention of Pudong New Area, Shanghai, China.

Neurological disorders are the leading cause of long-term disability and the second leading cause of death in the world. We aimed to characterize the long-term trends in mortality and disease burden of selected neurological disorders and quantitatively analyze the contributions of demographic and non-demographic factors on the mortality of selected neurological disorders in Shanghai, China, 1995-2018. Mortality data were derived from the Vital Statistics System of Pudong New Area, Shanghai, China, during 1995-2018. Temporal trends for the mortality rates and burden of selected neurological disorders were analyzed by Joinpoint Regression Program. Years of life lost (YLL) was used to analyze the burden of disease. The increasing mortality rates related to demographic and non-demographic factors were estimated by the decomposition method. A total of 4432 deaths from selected neurological disorders occurred during 1995-2018, accounting for 0.98% of total deaths. The crude mortality rates (CMR) and age-standardized mortality rates (ASMRW) of neurological disorders were 7.14/10 person-years and 4.08/10 person-years, respectively. Extrapyramidal and movement disorders, other degenerative diseases of the nervous system, and episodic and paroxysmal disorders were the three leading causes of mortality and YLL of selected neurological disorders. The CMR, ASMRW, and rate of YLL for deaths from selected neurological disorders showed significantly increasing trends in males, females, and the total population during 1995-2018 (all < 0.001). The contribution rates of increased values of CMR related to demographic factors were more evident than non-demographic factors. The mortality rate and rate of YLL for death from selected neurological disorders increased significantly during 1995-2018 in Pudong New Area, Shanghai. The demographic factors, particularly aging, might be related to an increase in the mortality of neurological disorders. More effective prevention strategies are needed to prevent the aging-related death and burden from neurological disorders in the future.
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http://dx.doi.org/10.3389/fneur.2021.625042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973274PMC
March 2021

The density of tumor-infiltrating lymphocytes and prognosis in resectable hepatocellular carcinoma: a two-phase study.

Aging (Albany NY) 2021 Mar 19;13(7):9665-9678. Epub 2021 Mar 19.

Department of Liver Surgery and Liver Transplantation, West China Hospital, Sichuan University, Chengdu, China.

Aim: Previous studies have focused on the subpopulations of tumor-infiltrating lymphocytes (TILs) in tumors. This study focuses only on the concentration of TILs in the tumor irrespective of type and elucidates its prognostic value.

Methods: We used 315 HCC patients as the discovery phase and another 343 HCC patients as the validation phase. By following the standardized guideline, density of TILs were categorized into low (TILs < 10%), intermediate (10% ≦ TILs < 50%), and high (TILs ≧ 50%) levels. Associations of TILs with prognostic, immune-related, and genetic variables were examined.

Results: We observed a dose-response relation of TILs with overall survival (intermediate: HR, 0.58; 95% confidence interval (CI), 0.36-0.93; high: HR, 0.37; 95% CI, 0.15-0.93) and disease-free survival (intermediate: HR, 0.35; 95% CI, 0.22-0.58; high: HR, 0.23; 95% CI, 0.09-0.58). The prognostic value of TILs was validated in the TCGA set. Mutation burden or the number of neoantigens were not associated with TILs intensity. However, hepatitis B or C virus infection patients had higher TILs intensity in the para-tumor tissue.

Conclusions: The TILs intensity was associated with patients' survival. If confirmed, this would suggest that clinical routine assessment of TILs could provide prognostic information in HCC.
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http://dx.doi.org/10.18632/aging.202710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064144PMC
March 2021

Neural-adjoint method for the inverse design of all-dielectric metasurfaces.

Opt Express 2021 Mar;29(5):7526-7534

All-dielectric metasurfaces exhibit exotic electromagnetic responses, similar to those obtained with metal-based metamaterials. Research in all-dielectric metasurfaces currently uses relatively simple unit-cell designs, but increased geometrical complexity may yield even greater scattering states. Although machine learning has recently been applied to the design of metasurfaces with impressive results, the much more challenging task of finding a geometry that yields a desired spectra remains largely unsolved. We propose and demonstrate a method capable of finding accurate solutions to ill-posed inverse problems, where the conditions of existence and uniqueness are violated. A specific example of finding the metasurface geometry which yields a radiant exitance matching the external quantum efficiency of gallium antimonide is demonstrated. We also show how the neural-adjoint method can intelligently grow the design search space to include designs that increasingly and accurately approximate the desired scattering response. The neural-adjoint method is not restricted to the case demonstrated and may be applied to plasmonics, photonic crystal, and other artificial electromagnetic materials.
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http://dx.doi.org/10.1364/OE.419138DOI Listing
March 2021

Efficacy of traditional herbal medicine versus transcatheter arterial chemoembolization in postsurgical patients with hepatocellular carcinoma: A retrospective study.

Complement Ther Clin Pract 2021 May 6;43:101359. Epub 2021 Mar 6.

School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, Shandong, China. Electronic address:

Background: Recurrence is a major obstacle to improve the prognosis of hepatocellular carcinoma (HCC) patients. Transcatheter arterial chemoembolization (TACE) has been routinely used as an adjuvant therapy in treating HCC, but efficacy of TACE in preventing the recurrence of HCC remains unsatisfactory. This study aimed to compare the efficacy of a traditional herbal medicine (THM) therapy and TACE in preventing tumor recurrence and improving survival in postsurgical patients with HCC.

Materials And Methods: A total of 1506 HCC patients were enrolled from January 2008 to June 2017, including 262 patients who received THM therapy and 1244 patients who were treated with TACE. All patients were followed up until the occurrence of outcome event or June 30th, 2019. The recurrence-free survival (RFS) and overall survival (OS) were calculated by the Kaplan-Meier method, and the differences of RFS and OS between THM group and TACE group were analyzed by the log-rank test. Factors affecting the RFS or OS among these patients were assessed by the Cox proportional hazard regression model. A nomogram was built with the factors based on the Cox regression analysis to predict the prognosis.

Results: The 1-, 3-, and 5-year RFS were 91.0%, 68.3%, and 49.7%, respectively, in the THM group and 79.4%, 38.6%, and 19.3%, respectively, in the TACE group. The RFS in the THM group was significantly higher than that of the TACE group (P = 6.2 × 10). The 1-, 3-, and 5-year OS were significantly improved in the THM group as compared to those in the TACE group (94.3%, 65.2%, and 41.4% vs. 82.7%, 46.0%, and 25.4%, P = 2.2 × 10). Multivariate analysis revealed that serum AFP level ≥400 ng/mL, HBV DNA load ≥500 copies/mL, TNM stage III-IV, tumor diameter ≥5 cm, presence of MVI, and multiple tumor nodules were independent risk factors for RFS, while complete tumor encapsulation and THM therapy were protective factors for RFS and OS. The nomogram demonstrated good accuracy in predicting RFS and OS, with the adjusted C-index of 0.748 and 0.796, respectively.

Conclusion: The efficacy of THM therapy was superior to that of TACE in preventing recurrence and improving survival for HCC patients after hepatectomy.
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http://dx.doi.org/10.1016/j.ctcp.2021.101359DOI Listing
May 2021

Association of body fat distribution and metabolic syndrome with the occurrence of colorectal adenoma: A case-control study.

J Dig Dis 2021 Apr 29;22(4):222-229. Epub 2021 Mar 29.

Department of Surgery, University of Hong Kong-Shenzhen Hospital, Shenzhen, Guangdong Province, China.

Objective: Visceral fat is thought to play different roles in the carcinogenesis of the colon with peripheral fat. Our aim was to evaluate the association of body fat distribution measured by bioelectrical impedance analysis (BIA) with the incidence of colorectal adenoma (CRA).

Methods: A total of 410 asymptomatic participants who underwent a screening colonoscopy from July 2017 to December 2019 in our center were recruited, including 230 with adenomas and 180 without detected adenomas. The participants' body fat was measured by BIA, including their body fat mass (BFM), body fat percentage (BFP), and waist-to-hip ratio. Parameters of metabolic syndrome (MetS), including waist circumference, blood pressure, fasting blood glucose (FBG), blood level of triglyceride, cholesterol, and high-density lipoprotein were measured as well.

Results: According to univariate analysis, age, male sex, body mass index, waist circumference, BFM, waist-to-hip ratio, blood pressure, and FBG were higher in the adenoma group than in the adenoma-free group (P < 0.05). On multivariate logistical analysis (adjusted for age, sex, smoking, drinking, and family history of CRC), a high waist-to-hip ratio was associated with a high incidence of CRA (odds ratio [OR] 1.84, 95% confidence interval [CI] 1.09-3.09, P = 0.02). Only a large waist circumference in components of MetS was independently associated with the incidence of CRA (OR 1.90, 95% CI 1.17-3.08, P = 0.01) in the multivariate analysis.

Conclusion: Body fat distribution is associated with CRA, central obesity is a core risk factor for CRA in MetS. Chinese Clinical Trial Registration number: ChiCTR-RRC-17010862.
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http://dx.doi.org/10.1111/1751-2980.12979DOI Listing
April 2021

Natural variations in a pectin acetylesterase gene, MdPAE10, contribute to prolonged apple fruit shelf life.

Plant Genome 2021 03 18;14(1):e20084. Epub 2021 Feb 18.

College of Horticulture, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China.

Room-temperature shelf life is a key factor in fresh market apple (Malus domestica Borkh.) quality and commercial value. To investigate the genetic and molecular mechanism underlying apple shelf life, quantitative trait loci (QTL) were identified using bulked segregant analysis via sequencing (BSA-seq). Ethylene emission, flesh firmness, or crispness of apple fruit from 1,273 F plants of M. asiatica Nakai 'Zisai Pearl' × M. domestica 'Golden Delicious' were phenotyped prior to and during 6 wk of room-temperature storage. Segregation of ethylene emission and the flesh firmness or crispness traits was detected in the population. Thirteen QTL, including three major ones, were identified on chromosome 03, 08, and 16. A candidate gene encoding pectin acetylesterase, MdPAE10, from the QTL Z16.1 negatively affected fruit shelf life. A 379-bp deletion in the coding sequence of MdPAE10 disrupted its function. A single nucleotide polymorphism (SNP) in the MdPAE10 promoter region reduced its transcription activity. These findings provided insight into the genetic control of fruit shelf life and can be potentially used in apple marker-assisted selection.
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http://dx.doi.org/10.1002/tpg2.20084DOI Listing
March 2021

Adverse Cardiovascular Effects of Phenylephrine Eye Drops Combined With Intravenous Atropine.

Front Pharmacol 2020 27;11:596539. Epub 2021 Jan 27.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

Phenylephrine and atropine can cause serious adverse effects when applied in combination. We investigated the effect of phenylephrine eye drops combined with intravenous atropine on the cardiovascular system in patients under general anesthesia undergoing intraocular surgery. The effects of the drugs were observed through clinical study. Thirteen patients undergoing intraocular surgery under general anesthesia were observed in this study; all were injected intravenously with atropine due to the oculocardiac reflex during surgery. To study the combination of drugs, an study was performed on rats. Seventy-two standard deviation rats that received phenylephrine eye drops and intravenous atropine treatment under general anesthesia were assessed, of which 18 treated with these drugs simultaneously were administered normal saline, neostigmine or esmolol. Blood pressure and heart rate were recorded and analyzed. The age of the patients ranged from seven to 14 years old with an average age of 10.7 years old, and 11 patients were male. In patients, 5% phenylephrine eye drops combined with intravenous atropine led to a significant heart rate increase and the increase lasted 20 min. The significant increase in diastolic blood pressure and systolic blood pressure lasted for 15 and 25 min, respectively. From five to 25 min after intravenous atropine treatment, the systolic blood pressure and diastolic blood pressure were both more than 20% higher than that at baseline. In rats, the changes in blood pressure and heart rate were independent of the phenylephrine and atropine administration sequence but were related to the administration time interval. The neostigmine group showed a significant decrease in blood pressure after the increase from the administration of phenylephrine and atropine. Phenylephrine eye drops combined with intravenous atropine have obvious cardiovascular effects that can be reversed by neostigmine. This drug combination should be used carefully for ophthalmic surgery, especially in patients with cardio-cerebrovascular diseases.
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http://dx.doi.org/10.3389/fphar.2020.596539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873643PMC
January 2021

A sensitive and high-throughput LC-ESI-MS/MS method to detect budesonide in human plasma: application to an evaluation of pharmacokinetics of budesonide intranasal formulations with and without charcoal-block in healthy volunteers.

Drug Dev Ind Pharm 2021 Feb 1;47(2):329-336. Epub 2021 Feb 1.

Department of Pharmacy, The Third Hospital of Changsha, Changsha, China.

Budesonide is one of the intranasal corticosteroids, referred as first-line therapy for allergic rhinitis. Its determination is a challenging task due to its extremely low plasma levels, which limits the progress in the investigation of pharmacokinetics and quality control of preparations. In this study, a sensitive and high-throughput method to determine budesonide in human plasma using budesonide-d8 as the internal standard was developed and validated. A small volume of plasma sample (0.2 mL) was diluted with 0.2 mL water, followed by a solid-phase extraction using Cleanert PEP-2 products. Extracted samples were analyzed by liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Chromatographic separation of analytes was performed on an InertSustain AQ-C18 HP column (3 µm, 2.1 × 50 mm) under the reversed-phase condition with gradient elution. With the assay, linear calibration curves were obtained over the concentration range of 10-1200 pg/mL for budesonide, with considerable extraction recoveries (84.7-89.4%), and negligible matrix effects (<4.1). Moreover, the newly developed method was successfully applied to the evaluation of pharmacokinetics of two budesonide intranasal formulations with and without charcoal block in healthy volunteers.
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http://dx.doi.org/10.1080/03639045.2021.1879842DOI Listing
February 2021

Copper in LaMnO to promote peroxymonosulfate activation by regulating the reactive oxygen species in sulfamethoxazole degradation.

J Hazard Mater 2021 Jun 16;411:125163. Epub 2021 Jan 16.

Department of Earth and Environmental Studies, Montclair State University, Montclair, NJ 07043, USA.

Perovskites with flexible texture structures and excellent catalytic properties have attracted considerable attention in peroxymonosulfate (PMS) activation for addressing organic contaminants in water. In this study, the role of copper to promote PMS activation performance of LaMnO was investigated. 100% sulfamethoxazole (SMX) degradation and 34% mineralization were achieved over copper doped LaMnO while only 60% SMX was removed without TOC removal by LaMnO. Especially, compared with LaMnO, the pseudo-first-order reaction rate constant was increased by 8.30 times when the atomic ratio of Cu/Mn was 1:3. It proved that only O was generated in LaMnO while O, especially •OH and SO• were all detected in Cu-LaMnO/PMS system. The characterization results showed that Cu induced the formation of LaMnO and LaCuO heterostructure with enhanced content of relatively low-valence Mn and Cu and abundant oxygen vacancies (OVs). Hence, the efficient PMS activation by Cu-LaMnO was due to regulating the produced reactive oxygen species (ROS). A radical dominant instead of O involved PMS activation mechanism over LaMnO-LaCuO was proposed for efficient degradation of SMX. Finally, the possible degradation pathways of SMX were discussed based on HPLC-MS analysis. This study provided a new insight of improving the catalytic activity of perovskites in PMS activation in water treatment.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125163DOI Listing
June 2021

Uterus inguinal hernia accompaniment didelphys uterus: A case report.

Int J Surg Case Rep 2020 19;77:570-572. Epub 2020 Nov 19.

Guizhou Medical University, Guizhou, China; Department of General Surgery, Guizhou Provincial People's Hospital, Guizhou, China. Electronic address:

Introduction: Inguinal hernia encompassing the uterus, ovary and fallopian tube are uncommonly present in women of reproductive age, which is often diagnosed and treated during childhood. In this article we report a case of uterus inguinal hernia accompaniment didelphys uterus were confirmed during surgery.

Presentation Of Case: A 15-year-old female presented with a history of a reducible mass located in the right inguinal region. Surgery was performed through an inguinal approach and the entire infantile uterus, both ovary and fallopian tube were found within the hernia sac. Laparoscopic exploration confirmed a normal uterus in the pelvic cavity, and the patient was discharged 3 days after the Lichtenstein operation.

Discussion: Although inguinal herniation is a common condition that occurs most often in elderly male and without any associated clinical symptoms. But uterine inguinal herniation should be considered as a reducible mass showed in the inguinal region. For open surgery, laparoscopy can be clearly diagnosed if it's necessary to avoid blindly cutting and ligating important structures, which may affect fertility function after operation.

Conclusion: Before the final choice of treatment is made, digestive surgeons should bear this rare hernia of uterine in mind for the differential diagnosis of an inguinal masses in women of child-bearing period. Congenital deformity genital organ, meanwhile, also should be considered.
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http://dx.doi.org/10.1016/j.ijscr.2020.11.069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708765PMC
November 2020

Role of MdERF3 and MdERF118 natural variations in apple flesh firmness/crispness retainability and development of QTL-based genomics-assisted prediction.

Plant Biotechnol J 2020 Dec 15. Epub 2020 Dec 15.

College of Horticulture, China Agricultural University, Beijing, China.

Retention of flesh texture attributes during cold storage is critical for the long-term maintenance of fruit quality. The genetic variations determining flesh firmness and crispness retainability are not well understood. The objectives of this study are to identify gene markers based on quantitative trait loci (QTLs) and to develop genomics-assisted prediction (GAP) models for apple flesh firmness and crispness retainability. Phenotype data of 2664 hybrids derived from three Malus domestica cultivars and a M. asiatica cultivar were collected in 2016 and 2017. The phenotype segregated considerably with high broad-sense heritability of 83.85% and 83.64% for flesh firmness and crispness retainability, respectively. Fifty-six candidate genes were predicted from the 62 QTLs identified using bulked segregant analysis and RNA-seq. The genotype effects of the markers designed on each candidate gene were estimated. The genomics-predicted values were obtained using pyramiding marker genotype effects and overall mean phenotype values. Fivefold cross-validation revealed that the prediction accuracy was 0.5541 and 0.6018 for retainability of flesh firmness and crispness, respectively. An 8-bp deletion in the MdERF3 promoter disrupted MdDOF5.3 binding, reduced MdERF3 expression, relieved the inhibition on MdPGLR3, MdPME2, and MdACO4 expression, and ultimately decreased flesh firmness and crispness retainability. A 3-bp deletion in the MdERF118 promoter decreased its expression by disrupting the binding of MdRAVL1, which increased MdPGLR3 and MdACO4 expression and reduced flesh firmness and crispness retainability. These results provide insights regarding the genetic variation network regulating flesh firmness and crispness retainability, and the GAP models can assist in apple breeding.
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http://dx.doi.org/10.1111/pbi.13527DOI Listing
December 2020

Decreases Bioavailability of Arsenic(V) via Biotransformation of Manganese Oxide into Biogenic Oxalate Minerals.

J Fungi (Basel) 2020 Nov 9;6(4). Epub 2020 Nov 9.

Institute of Laboratory Research on Geomaterials, Faculty of Natural Sciences, Comenius University in Bratislava, 84215 Bratislava, Slovakia.

The aim of this work was to evaluate the transformation of manganese oxide (hausmannite) by microscopic filamentous fungus and the effects of the transformation on mobility and bioavailability of arsenic. Our results showed that the strain CBS 140837 greatly affected the stability of hausmannite and induced its transformation into biogenic crystals of manganese oxalates-falottaite and lindbergite. The transformation was enabled by fungal acidolysis of hausmannite and subsequent release of manganese ions into the culture medium. While almost 45% of manganese was bioextracted, the arsenic content in manganese precipitates increased throughout the 25-day static cultivation of fungus. This significantly decreased the bioavailability of arsenic for the fungus. These results highlight the unique strain's ability to act as an active geochemical factor via its ability to acidify its environment and to induce formation of biogenic minerals. This affects not only the manganese speciation, but also bioaccumulation of potentially toxic metals and metalloids associated with manganese oxides, including arsenic.
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http://dx.doi.org/10.3390/jof6040270DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711977PMC
November 2020

Electrochemical oxidation of aniline using Ti/RuO-SnO and Ti/RuO-IrO as anode.

Chemosphere 2021 Apr 25;269:128734. Epub 2020 Oct 25.

School of Water Resources and Environment, China University of Geosciences (Beijing), No.29 Xueyuan Road, Haidian District, Beijing, 100083, China.

Electrocatalytic properties of anode and the electrolyte composition are important parameters influence the degradation efficiency for aniline wastewater. Ti/RuO-SnO and Ti/RuO-IrO have been fabricated using thermal decomposition method and experiments in electrolyte containing 0.05 M NaSO, 0.05 M NaCl and 0.05 M NaSO+0.005 M FeSO at different current density were conducted to study the influence on aniline degradation. Linear sweep voltammetry (LSV) showed that Ti/RuO-SnO had higher oxygen evolution potential and degrade aniline through electrochemical transformation and electrochemical combustion while Ti/RuO-IrO degrade aniline mainly through electrochemical transformation. The study showed that Ti/RuO-SnO had higher electrocatalytic activity towards the degradation of aniline than Ti/RuO-IrO anode in 0.05 M NaSO and in 0.05 M NaCl electrolyte. The maximum TOC removal efficiency for Ti/RuO-SnO was 64.2% at 40 mA cm in NaSO electrolyte while the average MCE was 1.6% and the average EC was 1.51 kWh (g TOC). On the contrary, the maximum TOC removal efficiency for Ti/RuO-IrO was 63.1% at 40 mA cm in NaCl electrolyte while the average MCE was 1.6% and the average EC was 1.95 kWh (g TOC). The presence of Fe in NaSO electrolyte would decrease the TOC removal efficiency except at low current density (20 mA cm) for Ti/RuO-SnO. These results indicated that Ti/RuO-SnO and Ti/RuO-IrO anode were suitable in NaSO and NaCl electrolyte, respectively, while the presence of Fe would inhibit aniline degradation.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128734DOI Listing
April 2021

Chemically enhanced primary treatment of municipal wastewater with ferrate(VI).

Water Environ Res 2020 Nov 1. Epub 2020 Nov 1.

Department of Earth and Environmental Studies, Montclair State University, Montclair, NJ, USA.

Chemically enhanced primary treatment (CEPT) with ferrate(VI), a multifunctional treatment agent, was investigated for the treatment of municipal wastewater in a laboratory-scale study. The treatment performance was evaluated at different ferrate(VI) doses (0.0-9.0 mg/L as Fe) and pH (6.0 and 7.5). The optimal removals of total suspended solids (TSS) (52%), total chemical oxygen demand (COD) (34%), and total phosphorus (47%) were achieved at the highest ferrate(VI) dose (9.0 mg/L as Fe) and the weakly alkaline condition (pH 7.5). The pollutant abatements principally ascribed to the formation of large-sized aggregate and ensuing sedimentation fell within the reported ranges of CEPT with traditional coagulants. However, different from conventional CEPT, ferrate(VI) appreciably removed recalcitrant dissolved organic phosphorus (49%) and simultaneously inactivated total coliform (3.30 log removal) and Escherichia coli (3.67 log removal) at 9.0 mg/L Fe(VI) and pH 7.5. The CEPT with ferrate(VI) offers an innovative alternative for improving municipal wastewater treatment. PRACTITIONER POINTS: Ferrate(VI) represents a promising agent for chemically enhanced primary treatment (CEPT) of municipal wastewater. CEPT with ferrate(VI) can effectively alleviate TSS, total COD, and total P via the formation of large-sized aggregates and ensuing sedimentation. Ferrate(VI) can substantially remove recalcitrant dissolved organic phosphorus in municipal wastewater. Different from other CEPT coagulants, ferrate(VI) can appreciably inactivate bacterial indicators during CEPT. Higher ferrate(VI) dose and weakly alkaline pH favor the performance of ferrate(VI) CEPT.
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http://dx.doi.org/10.1002/wer.1473DOI Listing
November 2020

Intensified Disinfection Amid COVID-19 Pandemic Poses Potential Risks to Water Quality and Safety.

Environ Sci Technol 2021 04 29;55(7):4084-4086. Epub 2020 Oct 29.

State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China.

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http://dx.doi.org/10.1021/acs.est.0c04394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641043PMC
April 2021

Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.

Int J Biol Macromol 2020 Dec 26;165(Pt B):2630-2639. Epub 2020 Oct 26.

College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.

Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions. Water-soluble pectic hydrocolloids from okra stems (HOS) were extracted and purified using polydivinylbenzene HP-20 resins. The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight. Its favorable rheological behaviors were evident on relatively higher concentrations (20, 25, and 30 mg/mL) and moderately lower pH levels (3 and 5) of HOS. The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels. HOS digestion in vivo was fairly conducive to the improvement of energy storage capacity and renal function for physically induced fatigue, compared with the conventional herbal supplement Panax quinquefolium. Accordingly, HOS exhibits potential for reutilization of okra stem waste.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.10.167DOI Listing
December 2020

Solution-Based Synthesis of Layered Two-Dimensional Oxides as Broadband Emitters.

ACS Nano 2020 Nov 19;14(11):15544-15551. Epub 2020 Oct 19.

Department of Materials Science and Engineering, University of California, Berkeley, California 94720, United States.

Preparing transition-metal oxides in their two-dimensional (2D) form is the key to exploring their unrevealed low-dimensional properties, such as the ptype transparent superconductivity, topological Mott insulator state, existence of the condensed 2D electron/hole gas, and strain-tunable catalysis. However, existing approaches suffer from the specific constraint techniques and precursors that limit their product types. Here, we report a solution-based method to directly synthesize KNbO in 2D by an out-of-the-pot growth process at low temperature, which is observed directly in real time. The developed method can also be applied to other 2D ternary oxide syntheses, including CsNbO and composited NaKNbO, and it can be extended to the preparation of self-assembled nanofilms. In addition, We demonstrate the emission of broadband photoluminescence (PL, λ ∼ 350-800 nm) from as-synthesized single-crystal 2D KNbO sheets down to a single unit cell thickness. The ultra-broadband emission is ascribed to the self-trapped excitation state (STEs) from the in-phase distortion of the NbO octahedrons in 2D NbO layers. Beyond the broader luminescent range and the robust material thermal stability of niobates, the absence of sample size restrictions and the large aspect ratio of the 2D oxide sheets will provide opportunities in miniaturizing and advancing 2D-materials integrated optoelectronic devices.
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http://dx.doi.org/10.1021/acsnano.0c06164DOI Listing
November 2020

Years of life lost due to premature death and their trends in people with malignant neoplasm of female genital organs in Shanghai, China during 1995-2018: a population based study.

BMC Public Health 2020 Oct 1;20(1):1489. Epub 2020 Oct 1.

Obstetrics and Gynecology Hospital of Fudan University, 419 Fangxie Road, Shanghai, 200011, China.

Background: The increasing aging population has been posing a significant challenge to disease burden in developing countries. In particular, the contribution of population aging to and long term changes of disease burden of malignant neoplasm of female genital organs (MNFGO) have not been quantitatively demonstrated.

Methods: Data were collected from the Shanghai Vital Statistics System of Pudong New Area (PNA). Crude mortality rate (CMR), age-standardized mortality rate by Segi's world standard population (ASMRW), and years of life lost (YLL) of MNFGO as the underlying cause of death in age and pathology types from 1995 to 2018 were calculated. The joinpoint regression was used to estimate the trends of those rates by identifying the annual percent changes (APCs), and the decomposition method was used to calculate the increased rates and the contribution resulting from demographic and non-demographic factors.

Results: From 1995 to 2018, a total of 2869 MNFGO-specific deaths were reported in PNA, accounting for 0.64% of the total deaths. The CMR and ASMRW of MNFGO were 9.23/10 person-years and 4.80/10 person-years, respectively. Ovary cancer was the most common cause of MNFGO death, accounting for 43.9% (1260/2869) of all MNFGO death. Other common causes of MNFGO death included cervix uteri cancer, uterus unspecified cancer, and corpus uteri cancer. With the increase of age, the mortality rate of MNFGO in residents had shown an upward trend ([APC (95%CI) = 3.46 (2.74, 4.18), P < 0.001)] for each five-year age group from 0 to 4 to 85+ years. From 1995 to 2018, YLL of MNFGO in Shanghai PNA was 42,152.82 years, and the rate of YLL was 135.56 /10. The top three MNFGO types in YLLs were ovary cancer, cervix uteri cancer and uterus unspecified cancer. Demographic factors contributed significantly to the upward trends of CMR, ASMRW, and YLL rates of MNFGO.

Conclusion: With aggravated population aging in Shanghai, MNFGO is and will continue to be a serious threat to women's health. More precise and effective prevention strategies are needed to target high risk population, to achieve efficient health resource allocation and to improve women's health in particular.
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http://dx.doi.org/10.1186/s12889-020-09593-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528500PMC
October 2020

Does the time from symptom onset to surgery affect the outcomes of patients with acute appendicitis? A prospective cohort study of 255 patients.

Asian J Endosc Surg 2020 Sep 29. Epub 2020 Sep 29.

Department of Surgery, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China.

Introduction: The objective of this study was to evaluate the impact of operative timing on outcomes of acute appendicitis.

Methods: This study examined adult patients who had presented to the hospital with acute appendicitis and had undergone appendectomy from December 2017 to February 2019. Time delay and outcomes of perforated and non-perforated appendicitis were compared. Patients were classified into five groups based on the period from symptom onset to operation: group 1, <24 hours; group 2, ≥24 and <48 hours; group 3, ≥48 and <72 hours; group 4, ≥72 and <96 hours; and group 5, ≥96 hours. The five groups were compared, with risk of perforation assessed in particular.

Results: A total of 255 patients were included in the analysis. Symptom duration, operative time, and length of postoperative hospital stay (P < .001) were significantly longer in the perforated group (n = 49) than in the non-perforated group (n = 206). The perforated group also had a higher conversion rate to open procedures (P = .002) and a higher rate of wound infection (P = .034). Group 1 had 53 patients, group 2 had 95 patients, group 3 had 57 patients, group 4 had 32 patients, and group 5 had 18 patients. The incidence of appendiceal perforation and median operative time progressively increased along with symptom duration in the five groups. In multivariate analyses, independent risk factors for appendiceal perforation were male gender (odds ratio = 2.33, 95% confidence interval [CI]: 1.07-5.08) and symptom duration ≥48 hours (relative to ≥24 and <48 hours) (odds ratio = 4.64, 95%CI: 1.76-12.27). Patients with symptom duration ≥72 hours had a significantly longer operative time than those with symptom duration ≥48 and <72 hours (β = 21.38, 95%CI: 5.66-37.11, P = .008).

Conclusion: The risk of perforation increased significantly 48 hours after the onset of appendicitis. Symptoms duration ≥72 hours was associated with a longer operative time.
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http://dx.doi.org/10.1111/ases.12870DOI Listing
September 2020

Genetic landscape and autoimmunity of monocytes in developing Vogt-Koyanagi-Harada disease.

Proc Natl Acad Sci U S A 2020 10 28;117(41):25712-25721. Epub 2020 Sep 28.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Centre, Sun Yat-sen University, 510060 Guangzhou, China;

Vogt-Koyanagi-Harada (VKH) disease is a systemic autoimmune disorder affecting multiple organs, including eyes, skin, and central nervous system. It is known that monocytes significantly contribute to the development of autoimmune disease. However, the subset heterogeneity with unique functions and signatures in human circulating monocytes and the identity of disease-specific monocytic populations remain largely unknown. Here, we employed an advanced single-cell RNA sequencing technology to systematically analyze 11,259 human circulating monocytes and genetically defined their subpopulations. We constructed a precise atlas of human blood monocytes, identified six subpopulations-including S100A12, HLA, CD16, proinflammatory, megakaryocyte-like, and NK-like monocyte subsets-and uncovered two previously unidentified subsets: HLA and megakaryocyte-like monocyte subsets. Relative to healthy individuals, cellular composition, gene expression signatures, and activation states were markedly alternated in VKH patients utilizing cell type-specific programs, especially the CD16 and proinflammatory monocyte subpopulations. Notably, we discovered a disease-relevant subgroup, proinflammatory monocytes, which showed a discriminative gene expression signature indicative of inflammation, antiviral activity, and pathologic activation, and converted into a pathologic activation state implicating the active inflammation during VKH disease. Additionally, we found the cell type-specific transcriptional signature of proinflammatory monocytes, ISG15, whose production might reflect the treatment response. Taken together, in this study, we present discoveries on accurate classification, molecular markers, and signaling pathways for VKH disease-associated monocytes. Therapeutically targeting this proinflammatory monocyte subpopulation would provide an attractive approach for treating VKH, as well as other autoimmune diseases.
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http://dx.doi.org/10.1073/pnas.2002476117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568339PMC
October 2020

sp. nov., from desert gravel soil.

Int J Syst Evol Microbiol 2020 Nov;70(11):5711-5716

Institute of Medicinal Biotechnology, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 10050, PR China.

A Gram-stain-negative, aerobic, non-motile, pink-pigmented, coccus bacterium, designated CPCC 101081, was isolated from a gravel soil sample collected from Badain Jara desert, PR China. Growth of the isolate occurred at 10-37 °C and pH 5.0-8.0, with optimal growth at 28-32 °C and pH 7.0, respectively. The major cellular fatty acids were summed feature 8 (Cω7/C ω6), summed feature 3 (C ω6/Cω7) and C2-OH. Q-10 was detected as the main respiratory quinone. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified phospholipid, an amino-containing lipid and an unidentified glycophospholipid were examined in the polar lipids extraction. The 16S rRNA gene sequence comparison of strain CPCC 101081 with the available sequences in the GenBank database showed that the isolate was closely related to members of the genus , with the highest similarity to DSM 14916 (97.4 %). In the phylogenetic trees based on 16S rRNA gene sequences and the core genomes, strain CPCC 101081 was included within the clade of the genus , representing a species level, with the closest neighbor of DSM 14916 . The genomic DNA G+C content was 68.7 mol%. The average nucleotide identity and the digital DNA-DNA hybridization values between strain CPCC 101081 and the related type strains of the genus were all far lower than the cut-off values for definition species. On the basis of above phenotypic and genotypic characteristics, strain CPCC 101081 is proposed to represent a novel species of the genus with the name sp. nov. strain CPCC 101081 (=KCTC 62852=NBRC 113512) is the type strain of the species.
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http://dx.doi.org/10.1099/ijsem.0.004467DOI Listing
November 2020

Enhancing Charge Separation through Oxygen Vacancy-Mediated Reverse Regulation Strategy Using Porphyrins as Model Molecules.

Small 2020 10 15;16(40):e2001752. Epub 2020 Sep 15.

Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, 730070, P. R. China.

Highly efficient charge separation has been demonstrated as one of the most significant steps playing decisive roles in enhancing the overall efficiency of photoelectrochemical (PEC) processes. In this study, by employing 5,10,15,20-tetrakis (4-carboxyphenyl) porphyrin-Ni (NiTCPP) as a prototype, an oxygen vacancy (Vo)-mediated reverse regulation strategy is proposed for tuning hole transfer, which in turn can accelerate the transport of electrons and thus enhancing charge separation. The optimal NiO/NiTCPP system exhibits much higher (≈40 times) photocurrent and longer (≈13 times) lifetime of charge carriers compared with those of pure NiTCPP. Furthermore, the electron transfer kinetic rate constant (K ) is quantitatively determined by an efficient scanning photoelectrochemical microscopy (SPECM). The K of the optimal system has a 5.7-fold improvement. In addition, the similar enhancement in charge separation from other semiconductors (CoTCPP and FeTCPP) are also observed, indicating that the Vo-mediated reverse regulation strategy is a promising pathway for tuning the properties of light harvesters in solar energy conversion.
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http://dx.doi.org/10.1002/smll.202001752DOI Listing
October 2020

Impacts of pre-oxidation on the formation of disinfection byproducts from algal organic matter in subsequent chlor(am)ination: A review.

Sci Total Environ 2021 Feb 27;754:141955. Epub 2020 Aug 27.

Department of Earth and Environmental Studies, Montclair State University, Montclair, NJ 07043, United States.

Algae cells and algal organic matter (AOM) present in algae impacted source water pose a serious threat to the safety of drinking water. Conventional water treatment processes poorly remove AOM that can transform to harmful disinfection byproducts (DBPs) during ensuing disinfection. This article offers a comprehensive review on the impacts of pre-oxidation on the formation of DBPs from AOM in subsequent chlor(am)ination. Various characterization techniques for algal cells and AOM are first overviewed with an effort to better understanding of correlation between the AOM properties and downstream DBP formation. Then, the present work reviews recent studies on application of different pre-oxidation technologies, such as chlor(am) ination, UV irradiation, ozonation, ferrate (VI), permanganate oxidation and UV-based advanced oxidation processes (AOPs), to remove algal cells and degrade AOM. Pre-oxidation can reduce the stability of algal cells and inactivate algal cells for promoting cell aggregation and thus favoring coagulation. Meanwhile, pre-oxidation can mitigate and degrade AOM into small molecular weight organic compounds to reduce DBP formation potential during subsequent chlor(am)ination. Finally, this review provides an overall evaluation on the applicability of different pre-oxidation processes, and identifies future research demands.
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http://dx.doi.org/10.1016/j.scitotenv.2020.141955DOI Listing
February 2021

Dihydromyricetin inhibits cell proliferation, migration, invasion and promotes apoptosis regulating miR-21 in Human Cholangiocarcinoma Cells.

J Cancer 2020 29;11(19):5689-5699. Epub 2020 Jul 29.

Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, China, 410011.

Dihydromyricetin, the most abundant natural flavonoid isolated from Ampelopsis grossedentata, exhibits broad anti-tumor effects. However, the effects of dihydromyricetin on cholangiocarcinoma remain unclear. This study examined the anti-tumor effects of dihydromyricetin in two human cholangiocarcinoma cell lines HCCC9810 and TFK-1, and the underlying mechanism was also investigated. Our study was the first to show that dihydromyricetin significantly inhibited cell proliferation, migration, invasion and promoted apoptosis in cholangiocarcinoma cells. By analyzing the TCGA dataset, we found that expression of miR-21, an oncogene and a potential target of anticancer drugs for cholangiocarcinoma, was upregulated in cholangiocarcinoma tissues compared to paired control tissues. Moreover, dihydromyricetin significantly reduced the expression of miR-21 in a dose-dependent manner. Overexpression of miR-21 remarkably abolished the inhibitory effects of dihydromyricetin on cell proliferation, migration, invasion and abrogated its effect of promoting cell apoptosis in both HCCC9810 and TFK-1 cells. Dihydromyricetin remarkably increased the expression of PTEN and decreased the expression of phosphorylated Akt, while overexpression of miR-21 abrogated the modulation of PTEN/ Akt pathway by dihydromyricetin. Taken together, our study demonstrates that dihydromyricetin inhibits cell proliferation, migration, invasion and promotes apoptosis in cholangiocarcinoma cells via regulating miR-21.
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http://dx.doi.org/10.7150/jca.45970DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477438PMC
July 2020

Glycomics: Immunoglobulin G -Glycosylation Associated with Mammary Gland Hyperplasia in Women.

OMICS 2020 09 24;24(9):551-558. Epub 2020 Aug 24.

School of Public Health, Shandong First Medical University and Shandong Academy of Medical Sciences, Taian, China.

Mammary gland hyperplasia (MGH) is very common, especially among young and middle-aged women. New diagnostics and biomarkers for MGH are needed for rational clinical management and precision medicine. We report, in this study, new findings using a glycomics approach, with a focus on immunoglobulin G (IgG) -glycosylation. A cross-sectional study was conducted in a community-based population sample in Beijing, China. A total of 387 participants 40-65 years of age were enrolled in this study, including 194 women with MGH (cases) and 193 women who had no MGH (controls). IgG -glycans were characterized in the serum by ultra-performance liquid chromatography. The levels of the glycan peaks (GPs) GP2, GP5, GP6, and GP7 were lower in the MGH group compared with the control group, whereas GP14 was significantly higher in the MGH group ( < 0.05). A predictive model using GP5, GP21, and age was established and a receiver operating characteristic curve analysis was performed. The sensitivity and specificity of the model for MGH was 61.3% and 63.2%, respectively, likely owing to receptor mechanisms and/or inflammation regulation. To the best of our knowledge, this is the first study reporting on an association between IgG -glycosylation and MGH. We suggest person-to-person variations in IgG -glycans and their combination with multiomics biomarker strategies offer a promising avenue to identify novel diagnostics and individuals at increased risk of MGH.
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http://dx.doi.org/10.1089/omi.2020.0091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480711PMC
September 2020