Publications by authors named "Yang Cheng"

1,213 Publications

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Electrochemiluminescent Chiral Discrimination with a Pillar[5]arene Molecular Universal Joint-Coordinated Ruthenium Complex.

Org Lett 2021 May 7. Epub 2021 May 7.

Key Laboratory of Green Chemistry & Technology of Ministry of Education, College of Chemistry, and Healthy Food Evaluation Research Center, Sichuan University, Chengdu 610064, China.

A bicyclic pillar[5]arene derivative fused with a bipyridine side ring, a so-called molecular universal joint (MUJ), was synthesized, and the pair of enantiomers was resolved by high-performance liquid chromatography enantioresolution. The electrochemiluminescent detection based on the ruthenium complex of the enantiopure MUJ showed excellent chiral discrimination toward certain amino acids.
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http://dx.doi.org/10.1021/acs.orglett.1c01016DOI Listing
May 2021

An update on genetic basis of generalized pustular psoriasis (Review).

Int J Mol Med 2021 Jun 6;47(6). Epub 2021 May 6.

Department of Laboratory Medicine, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China.

Generalized pustular psoriasis (GPP) is a rare and severe auto‑inflammatory skin disease that is characterized by recurrent, acute onset, and generalized pustular eruptions on erythematous, inflamed skin. GPP is traditionally classified as a variant of psoriasis vulgaris, even though recent clinical, histological and genetic evidence suggests that it is a heterogeneous disease and requires a separate diagnosis. In recent years, variants of , , and genes have been identified as causative or contributing to genetic defects in a proportion of patients affected by GPP. These disease‑related genes are involved in common inflammatory pathways, in particular in the IL‑1/IL‑36‑chemokines‑neutrophil pathogenic axis. At present, no standard therapeutic guidelines have been established for GPP management, and there is a profound need for novel efficacious treatments of GPP. Among them, biological agents antagonizing the IL‑36 pathway are promising therapeutics. The aim of the present review is to provide the most recent updates on the genetics, genotype‑phenotype correlation and pathological basis of GPP, as well as on biologic treatments available for GPP and relative clinical courses.
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http://dx.doi.org/10.3892/ijmm.2021.4951DOI Listing
June 2021

-Toluenesulfonic acid functionalized imidazole ionic liquids encapsulated into bismuth SBA-16 as high-efficiency catalysts for Friedel-Crafts acylation reaction.

Dalton Trans 2021 May;50(17):5871-5882

School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, P. R. China.

Bismuth SBA-16 catalyst was synthesized by the hydrothermal method. Four kinds of p-toluenesulfonic acid functionalized imidazole ionic liquids were prepared by a two-step method and their molecular structures were characterized by 1H NMR and MS. The post-synthesis impregnation method was used to functionalize the Bi(10)-SBA-16 silicon mesoporous material with the ionic liquids and the obtained materials were characterized by FT-IR, XRD, BET, XPS, and TG. The results show that the volume and pore size of SBA-16 were changed by loading Bi and ionic liquids, while the three-dimensional cubic pore structure of SBA-16 was not destroyed. The composite catalyst was evaluated in Friedel-Crafts acylation of anisole with acetic anhydride. The effects of reaction temperature and the ratio of anisole and acylating agent on the acylation of anisole were investigated by experimental design. The results showed that 1.2ILc@Bi(10)-SBA-16 was used as the catalyst, the conversion of anisole was 85.41% and the yield of aromatic ketone was 69.19% under the conditions of a reaction temperature of 100 °C, a catalyst dosage of 0.5 g, a time period of 4 h and a molar ratio of 1 : 1.5. After 5 recycling runs, the reduction in the overall ratio of reactant conversion and product yield did not exceed 7.5%, indicating that 1.2ILc@Bi(10)-SBA-16 has good stability and reusability.
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http://dx.doi.org/10.1039/d1dt00355kDOI Listing
May 2021

Three-Random-Point Marking Method for Toric Intraocular Lens Alignment Using the iTrace Aberrometer.

J Ophthalmol 2021 8;2021:6695454. Epub 2021 Apr 8.

Department of Ophthalmology, Guangdong Eye Institute, Guangdong Academy of Medical Sciences, Guangdong Provincial People's Hospital, Guangzhou, China.

Purpose: To evaluate the clinical outcome of the three-random-point (TRP) marking method for toric intraocular lens (IOL) alignment using the iTrace aberrometer (Tracey Technologies Corp., Houston, TX). . Department of Ophthalmology, Guangdong Eye Institute, Guangdong Academy of Medical Sciences, Guangdong Provincial People's Hospital, Guangzhou, China.

Design: Prospective, randomized comparative trial.

Method: Thirty eyes of 30 patients undergoing cataract surgery with coexisting corneal astigmatism of over 1.0 D were included in this study. All patients were prospectively randomized into the TRP marking group or slit-lamp horizontal meridian (SHM) marking group. TRP marking involved marking three points randomly in the corneal limbus of the patients and accurately marking the horizontal meridian was not required. The follow-up duration was 3 months after cataract surgery.

Results: Fifteen eyes of 15 patients were in the TRP marking group and 15 eyes of 15 patients in the SHM marking group. There was no statistically significant difference in BCVA, UDVA, preexisting corneal astigmatism, or residual astigmatism between the groups before or after surgery ( > 0.05). The mean toric IOL misalignment was lesser but without significance in the TRP marking group than in the SHM marking group after 3 postoperative months (2.66° ± 1.42° versus 3.29° ± 1.67°; =0.295).

Conclusion: The TRP marking method using the iTrace aberrometer is simple and accurate for preoperative marking of toric IOLs. It can eliminate the potential systematic errors resulting from varying head positions during the preoperative keratometry measurement and from manual marking.
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http://dx.doi.org/10.1155/2021/6695454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049806PMC
April 2021

Asperuloside Enhances Taste Perception and Prevents Weight Gain in High-Fat Fed Mice.

Front Endocrinol (Lausanne) 2021 13;12:615446. Epub 2021 Apr 13.

School of Health Sciences, University of Tasmania, Newnham, TAS, Australia.

Asperuloside is an iridoid glycoside found in many medicinal plants that has produced promising anti-obesity results in animal models. In previous studies, three months of asperuloside administration reduced food intake, body weight, and adipose masses in rats consuming a high fat diet (HFD). However, the mechanisms by which asperuloside exerts its anti-obesity properties were not clarified. Here, we investigated homeostatic and nutrient-sensing mechanisms regulating food intake in mice consuming HFD. We confirmed the anti-obesity properties of asperuloside and, importantly, we identified some mechanisms that could be responsible for its therapeutic effect. Asperuloside reduced body weight and food intake in mice consuming HFD by 10.5 and 12.8% respectively, with no effect on mice eating a standard chow diet. Fasting glucose and plasma insulin were also significantly reduced. Mechanistically, asperuloside significantly reduced hypothalamic mRNA ghrelin, leptin, and pro-opiomelanocortin in mice consuming HFD. The expression of fat lingual receptors (CD36, FFAR1-4), CB1R and sweet lingual receptors (TAS1R2-3) was increased almost 2-fold by the administration of asperuloside. Our findings suggest that asperuloside might exert its therapeutic effects by altering nutrient-sensing receptors in the oral cavity as well as hypothalamic receptors involved in food intake when mice are exposed to obesogenic diets. This signaling pathway is known to influence the subtle hypothalamic equilibrium between energy homeostasis and reward-induced overeating responses. The present pre-clinical study demonstrated that targeting the gustatory system through asperuloside administration could represent a promising and effective new anti-obesity strategy.
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http://dx.doi.org/10.3389/fendo.2021.615446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076851PMC
April 2021

Ellagic Acid Attenuates BLM-Induced Pulmonary Fibrosis via Inhibiting Wnt Signaling Pathway.

Front Pharmacol 2021 12;12:639574. Epub 2021 Apr 12.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, China.

Idiopathic pulmonary fibrosis is a progressive lung disease with high mortality and limited therapy that is characterized by epithelial cell damage and fibroblast activation. Ellagic acid is a natural polyphenol compound widely found in fruits and nuts that has multiple pharmacological activities. In this study, we explored the potential effects and mechanisms of Ellagic acid on pulmonary fibrosis and . studies showed that Ellagic acid significantly alleviated bleomycin (BLM)-induced pulmonary fibrosis in mice. experiments indicated that Ellagic acid could suppress Wnt signaling and attenuate Wnt3a-induced myofibroblast activation and the phosphorylation of Erk2 and Akt. Further studies showed that Ellagic acid could induce autophagy formation in myofibroblasts mainly by suppressing mTOR signaling and promoting apoptosis of myofibroblasts. experiments revealed that Ellagic acid significantly inhibited myofibroblast activation and promoted autophagy formation. Taken together, our results show that Ellagic acid effectively attenuates BLM-induced pulmonary fibrosis in mice by suppressing myofibroblast activation and promoting autophagy and apoptosis of myofibroblasts by inhibiting the Wnt signaling pathway.
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http://dx.doi.org/10.3389/fphar.2021.639574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072668PMC
April 2021

Structures of Foot-and-mouth Disease Virus with neutralizing antibodies derived from recovered natural host reveal a mechanism for cross-serotype neutralization.

PLoS Pathog 2021 Apr 28;17(4):e1009507. Epub 2021 Apr 28.

MOE Key Laboratory of Protein Science & Collaborative Innovation Center of Biotherapy, School of Medicine and School of Life Sciences, Tsinghua University, Beijing, China.

The development of a universal vaccine against foot-and-mouth disease virus (FMDV) is hindered by cross-serotype antigenic diversity and by a lack of knowledge regarding neutralization of the virus in natural hosts. In this study, we isolated serotype O-specific neutralizing antibodies (NAbs) (F145 and B77) from recovered natural bovine hosts by using the single B cell antibody isolation technique. We also identified a serotype O/A cross-reacting NAb (R50) and determined virus-NAb complex structures by cryo-electron microscopy at near-atomic resolution. F145 and B77 were shown to engage the capsid of FMDV-O near the icosahedral threefold axis, binding to the BC/HI-loop of VP2. In contrast, R50 engages the capsids of both FMDV-O and FMDV-A between the 2- and 5-fold axes and binds to the BC/EF/GH-loop of VP1 and to the GH-loop of VP3 from two adjacent protomers, revealing a previously unknown antigenic site. The cross-serotype neutralizing epitope recognized by R50 is highly conserved among serotype O/A. These findings help to elucidate FMDV neutralization by natural hosts and provide epitope information for the development of a universal vaccine for cross-serotype protection against FMDV.
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http://dx.doi.org/10.1371/journal.ppat.1009507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081260PMC
April 2021

Optical zoom imaging systems using adaptive liquid lenses.

Bioinspir Biomim 2021 Apr 27. Epub 2021 Apr 27.

Beijing Institute of Technology, No.5 Yard,Zhong Guan Cun South Street,Haidian District, Beijing, Beijing, 100081, CHINA.

An optical zoom imaging system that can vary the magnification factor without displacing the object and the image plane has been widely used. Nonetheless, conventional optical zoom imaging systems suffer slow response, complicated configuration, vulnerability to misalignment during zoom operation, and are incompatible for miniaturized applications. This review article focuses on state-of-the-art research on novel optical zoom imaging systems that use adaptive liquid lenses. From the aspect of the configuration, according to the amount of the adaptive liquid lenses, we broadly divide the current optical zoom imaging systems using adaptive liquid lenses into two configurations: multiple adaptive liquid lenses, and a single adaptive liquid lens. The principles and configurations of these optical zoom imaging systems are introduced and represented. Three different working principles of the adaptive liquid lens (liquid crystal, polymer elastic membrane, and electrowetting effect) adopted in the optical zoom imaging systems are reviewed. Some representative applications of optical zooming imaging systems using adaptive liquid lenses are introduced. The opportunities and challenges of the optical zoom imaging systems using adaptive liquid lenses are also discussed. This review aims to provide a snapshot of the current state of this research field for attracting more attention to put forward the development of the next-generation optical zoom imaging systems.
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http://dx.doi.org/10.1088/1748-3190/abfc2bDOI Listing
April 2021

fucoidan modifies gut microbiota and intestinal metabolites during alleviation of hyperglycemia in type 2 diabetic mice.

Food Funct 2021 Apr;12(8):3572-3585

College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China.

Type 2 diabetic mellitus (T2DM) is a complicated metabolic disorder that is now considered as a major global public health problem. Fucoidan exhibits diverse biological activities, especially prevention of metabolic diseases. In this regard, we herein aimed to reveal the beneficial effect of Sargassum fusiforme fucoidan (SFF) on high-fat diet (HFD) and streptozotocin (STZ) induced T2DM mice. We noted that on the one hand, SFF significantly decreased fasting blood glucose, diet and water intake, and hyperlipidemia, while on the other hand, it improved glucose tolerance. Furthermore, SFF reduced epididymal fat deposition, attenuated the pathological changes in heart and liver tissues, and decreased oxidative stress in diabetic mice. To explore the underlying mechanisms of these ameliorative effects, the gut microbiota was analyzed. Notably, SFF highly enriched benign microbes including Bacteroides, Faecalibacterium and Blautia, as well as increased levels of (R)-carnitine and choline in the colon of diabetic mice. This may be a potential mechanism for alleviating T2DM, thus implying the benefits of SFF as an adjuvant agent for T2DM treatment. Taken together, this study demonstrated a promising application of fucoidan as one of the adjuvant agents for the management of T2DM in the future.
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http://dx.doi.org/10.1039/d0fo03329dDOI Listing
April 2021

Aggressive surgical procedures on endogenous Aspergillus endophthalmitis after heart transplantation.

Asian J Surg 2021 Apr 21. Epub 2021 Apr 21.

Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2021.03.046DOI Listing
April 2021

Subtyping Salmonella isolated from pet dogs with multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs).

AMB Express 2021 Apr 24;11(1):60. Epub 2021 Apr 24.

Jiangsu Co-Innovationnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, Jiangsu, China.

Salmonella, as a zoonotic pathogen, has attracted widespread attention worldwide, especially in the transmission between household pets and humans. Therefore, we investigated the epidemic distribution of dog Salmonella from pet hospitals and breeding base in Xuzhou, Jiangsu Province, China, and used multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs) to subtype Salmonella isolates. From April 2018 to November 2019, a total of 469 samples were collected from pet hospitals and breeding base, including 339 dog samples and 60 cat samples. S. Kentucky (40.74%) was the most prevalent serotype, but other, such as S. Typhimurium (18.52%) and S. Indiana (18.52%), were also widespread. Eight different sequence type (ST) patterns were identified by MLST and ST198 was the highest proportion of these isolates. CRISPRs analysis showed that 9 different Kentucky CRISPR types (KCTs) was identified from ST198. 48 spacers including 29 (6 News) for CRISPR1 and 19 (4 News) for CRISPR2 that proved the polymorphic of Salmonella genes in samples from different sources. The analysis demonstrated that the common serotypes were widely present in pet hosts in the same area. This analysis shows that CRISPR genes have better recognition ability in the same serotype, which has a positive effect on the traceability of Salmonella and the prevention and treatment of salmonellosis.
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http://dx.doi.org/10.1186/s13568-021-01221-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068741PMC
April 2021

Factors Related to Instrumentation Failure in Titanium Mesh Reconstruction for Thoracic and Lumbar Tumors: Retrospective Analysis of 178 Patients.

Cancer Manag Res 2021 15;13:3345-3355. Epub 2021 Apr 15.

Department of Orthopedics, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, People's Republic of China.

Purpose: To investigate risk factors for instrumentation failure (IF) in titanium (Ti) mesh reconstruction for thoracic and lumbar tumors.

Patients And Methods: The clinical data of patients with thoracic or lumbar tumors who received Ti mesh reconstruction via the posterior approach in our hospital from 2013 to 2018 were analyzed retrospectively. The observation indexes included sex, age, BMI, the vertebra resection mode, the number of resected vertebral segments, application of bone cement, radiotherapy, chemotherapy, revision or primary surgery, and primary tumor metastasis. Correlations between these factors and IF were analyzed by Kaplan-Meier survival and logistics regression analyses.

Results: The 178 patients included 108 males and 70 females with a mean age of 48.09±16.21 (6-78) years and a mean follow-up period of 51.18 (24-90) months. The data showed that 17 patients (9.55%) were inflicted with IF, involving the thoracic vertebra in 11 cases, thoracolumbar vertebrae (T12-L1) in 2 cases, and lumbar vertebrae in 4 cases. The mean interval between surgery to IF was 35.18±14.17 (14-59) months. Univariate analysis showed that total vertebral body resection, the number of resected vertebral segments, radiotherapy and multiple tumor resection were potential factors for IF, while multivariate analysis showed that only total vertebral body resection, the number of resected vertebral segments and radiotherapy were independent factors.

Conclusion: Total vertebra resection, the number of resected vertebral segments (≥2) and radiotherapy before and after operation were significant risk factors related to IF.
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http://dx.doi.org/10.2147/CMAR.S294616DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055544PMC
April 2021

Prognostic role of proliferating CD8 cytotoxic Tcells in human cancers.

Cell Oncol (Dordr) 2021 Apr 17. Epub 2021 Apr 17.

Institute of Pathology, University Medical Centre Hamburg-Eppendorf, Martinistraße 52, D-20246, Hamburg, Germany.

Purpose: Expansion of CD8 cytotoxic Tlymphocytes is a prerequisite for anti-cancer immune activity and has gained interest in the era of immune checkpoint therapy.

Methods: To understand the CD8 T cell dynamics in the tumor microenvironment, we used multiplex fluorescence immunohistochemistry to quantitate CD8 proliferation (Ki67 co-expression) in tissue microarrays from 1107 colorectal, 642 renal cell, 1066 breast, 375 ovarian, 451 pancreatic and 347 gastric cancer samples.

Results: The density and the percentage of proliferating (Ki67) CD8 T cells were both highly variable between tumor types as well as between patients with the same tumor type. Elevated density and percentage of proliferating CD8 cytotoxic T cells were significantly associated with favorable tumor parameters such as low tumor stage, negative nodal stage (p ≤ 0.0041 each), prolonged overall survival (p ≤ 0.0028 each) and an inflamed immune phenotype (p = 0.0025) in colorectal cancer and, in contrast, linked to high tumor stage, advanced ISUP/Fuhrman/Thoenes grading (each p ≤ 0.003), shorter overall survival (p ≤ 0.0330 each) and an immune inflamed phenotype (p = 0.0094) in renal cell cancer. In breast, ovarian, pancreatic and gastric cancer the role of (Ki67)CD8 Tcells was not linked to clinicopathological data.

Conclusion: Our data demonstrate a tumor type dependent prognostic impact of proliferating (Ki67)CD8 Tcells and an inverse impact in colorectal and renal cell cancer.
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http://dx.doi.org/10.1007/s13402-021-00601-4DOI Listing
April 2021

Polymorph Evolution Mechanisms and Regulation Strategies of Lithium Metal Anode under Multiphysical Fields.

Chem Rev 2021 Apr 16. Epub 2021 Apr 16.

Institute of Materials Research, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.

Lithium (Li) metal, a typical alkaline metal, has been hailed as the "holy grail" anode material for next generation batteries owing to its high theoretical capacity and low redox reaction potential. However, the uncontrolled Li plating/stripping issue of Li metal anodes, associated with polymorphous Li formation, "dead Li" accumulation, poor Coulombic efficiency, inferior cyclic stability, and hazardous safety risks (such as explosion), remains as one major roadblock for their practical applications. In principle, polymorphous Li deposits on Li metal anodes includes smooth Li (film-like Li) and a group of irregularly patterned Li (, whisker-like Li (Li whiskers), moss-like Li (Li mosses), tree-like Li (Li dendrites), and their combinations). The nucleation and growth of these Li polymorphs are dominantly dependent on multiphysical fields, involving the ionic concentration field, electric field, stress field, and temperature field, . This review provides a clear picture and in-depth discussion on the classification and initiation/growth mechanisms of polymorphous Li from the new perspective of multiphysical fields, particularly for irregular Li patterns. Specifically, we discuss the impact of multiphysical fields' distribution and intensity on Li plating behavior as well as their connection with the electrochemical and metallurgical properties of Li metal and some other factors (, electrolyte composition, solid electrolyte interphase (SEI) layer, and initial nuclei states). Accordingly, the studies on the progress for delaying/suppressing/redirecting irregular Li evolution to enhance the stability and safety performance of Li metal batteries are reviewed, which are also categorized based on the multiphysical fields. Finally, an overview of the existing challenges and the future development directions of metal anodes are summarized and prospected.
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http://dx.doi.org/10.1021/acs.chemrev.0c01100DOI Listing
April 2021

Macrophages with reduced expressions of classical M1 and M2 surface markers in human bronchoalveolar lavage fluid exhibit pro-inflammatory gene signatures.

Sci Rep 2021 Apr 15;11(1):8282. Epub 2021 Apr 15.

St Paul's Hospital, The University of British Columbia (UBC) Centre for Heart Lung Innovation (HLI), Vancouver, BC, Canada.

The classical M1/M2 polarity of macrophages may not be applicable to inflammatory lung diseases including chronic obstructive pulmonary disease (COPD) due to the complex microenvironment in lungs and the plasticity of macrophages. We examined macrophage sub-phenotypes in bronchoalveolar lavage (BAL) fluid in 25 participants with CD40 (a M1 marker) and CD163 (a M2 marker). Of these, we performed RNA-sequencing on each subtype in 10 patients using the Illumina NextSeq 500. Approximately 25% of the macrophages did not harbor classical M1 or M2 surface markers (double negative, DN), and these cells were significantly enriched in COPD patients compared with non-COPD patients (46.7% vs. 14.5%, p < 0.001). 1886 genes were differentially expressed in the DN subtype compared with  all other subtypes at a 10% false discovery rate. The 602 up-regulated genes included 15 mitochondrial genes and were enriched in 86 gene ontology (GO) biological processes including inflammatory responses. Modules associated with cellular functions including oxidative phosphorylation were significantly down-regulated in the DN subtype. Macrophages in the human BAL fluid, which were negative for both M1/M2 surface markers, harbored a gene signature that was pro-inflammatory and suggested dysfunction in cellular homeostasis. These macrophages may contribute to the pathogenesis and manifestations of inflammatory lung diseases such as COPD.
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http://dx.doi.org/10.1038/s41598-021-87720-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050093PMC
April 2021

Carotenoid composition and antioxidant activities of Chinese orange-colored tomato cultivars and the effects of thermal processing on the bioactive components.

J Food Sci 2021 Apr 15. Epub 2021 Apr 15.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.

To facilitate the production of tomato products with high bioactivity and improve the utilization of orange-colored tomatoes, the carotenoids of 11 tomato cultivars were analyzed using high-performance liquid chromatography with photodiode array detection. Moreover, antioxidant activities were evaluated by four chemical-based assays, and the influences of thermal treatment on the carotenoids in orange-colored tomatoes rich in tetra-cis (7Z, 9Z, 7'Z, and 9'Z)-lycopene, phytofluene, and phytoene were studied. The nine orange-colored tomatoes (OT) were divided into two categories: OT-B, containing five cultivars rich in β-carotene, and OT-L, containing the other four cultivars that were abundant in tetra-cis-lycopene, phytofluene, and phytoene. The antioxidant activities of OT-L were higher than those of OT-B and the SD-2 cultivar in OT-L showed similar antioxidant activity to the red tomatoes. During thermal processing, tetra-cis-lycopene in SD-2 decreased about 38% after being exposed to heat for 2 hr at 80 °C, while its content was still higher than other lycopene isomers. Other-Z-lycopenes and all-trans (E)-lycopene increased from 2.36 ± 0.19 to 14.73 ± 1.16 µg/g fresh weight (FW) and 0.75 ± 0.10 to 5.91 ± 1.02 µg/g FW, respectively. Thus, thermal treatments at lower temperature, such as cold break and pasteurization, were more suitable for processing OT-L. The results demonstrated that OT-L could be an excellent raw material to produce tomato products with high bioavailability and bioactivity. The results of this research could provide helpful information for the research and development of tomato products using orange tomatoes and benefit planters and consumers. PRACTICAL APPLICATION: Some orange tomato cultivars are promising raw materials for tomato products because of their high contents of bioactive tetra-cis-lycopene, phytofluene, and phytoene. This study demonstrated the carotenoid components and antioxidant activities of the widely planted orange-colored tomatoes in China. The obtained knowledge, including the thermal processing effects on the isomerization and degradation of carotenoids in the cultivars, will offer useful information to food processors and benefit the consumers.
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http://dx.doi.org/10.1111/1750-3841.15682DOI Listing
April 2021

Correction to: LncRNA APCDD1L-AS1 induces icotinib resistance by inhibition of EGFR autophagic degradation via the miR-1322/miR-1972/ miR-324-3p-SIRT5 axis in lung adenocarcinoma.

Biomark Res 2021 Apr 14;9(1):25. Epub 2021 Apr 14.

Department of Respiratory and Infectious Disease of Geriatrics, The First Hospital of China Medical University, No.155 Nanjing North Street, Heping District, Shenyang, 110001, Liaoning, China.

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http://dx.doi.org/10.1186/s40364-021-00279-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048049PMC
April 2021

Nuclear translocation of ASPL-TFE3 fusion protein creates favorable metabolism by mediating autophagy in translocation renal cell carcinoma.

Oncogene 2021 May 12;40(18):3303-3317. Epub 2021 Apr 12.

Department of Pathology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu, China.

The ASPL-TFE3 fusion gene, resulting from t(X;17)(p11.2;q25.3), is one of the most commonly identified fusion genes in Xp11 translocation renal cell carcinoma (tRCC). However, its roles and underlying mechanism in RCC development are not yet clear. Here, we identified ASPL-TFE3 fusion as the most common tRCC subtype in a Chinese population (29/126, 23.03%). This fusion protein translocated into the nucleus and promoted RCC cell proliferation both in vitro and in vivo. Mechanistically, the fusion protein transcriptionally activated the lysosome-autophagy pathway by binding to the promoters of lysosome-related genes. Autophagy, activated by ASPL-TFE3, enabled RCC cells to escape energy stress by promoting the utilization of proteins and lipids. Moreover, we found that the ASPL-TFE3 fusion escaped regulation by the classic mTOR-TFE3 signal and instead activated phospho-mTOR and its downstream targets. Finally, targeting both autophagy and the mTOR axis resulted in a greater antiproliferative effect than single pathway inhibition. In summary, these results confirmed the ASPL-TFE3 fusion as a master regulator of metabolic adaptation mediated by autophagy in tRCC. The simultaneous manipulation of autophagy and the mTOR axis may represent a novel treatment strategy for ASPL-TFE3 fusion RCC.
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http://dx.doi.org/10.1038/s41388-021-01776-8DOI Listing
May 2021

Time-dependence and comparison of regional and overall anthropometric features between Asian and Caucasian populations with obstructive sleep apnea: a cumulative meta-analysis.

J Thorac Dis 2021 Mar;13(3):1746-1759

Interdisciplinary Center of Sleep Medicine, Charité University Hospital, Berlin, Germany.

Background: Anthropometric measurements are simple and reachable tools for self-evaluating and screening patients with a high risk of obstructive sleep apnea (OSA). However, the accumulated relationship of obesity on the anthropometric characteristics of OSA is not well understood. The aim of the study was to show the time-dependent trend of OSA patients and compare overall and regional anthropometric between two ethnicities.

Methods: A cumulative meta-analysis was performed to assess obesity metrics in patients with and without OSA between Asians and Caucasians. We searched PubMed, Web of Science, Embase, and Scopus up to Jun 2020. Included studies used body mass index (BMI), neck circumference (NC), waist circumference (WC) and waist-to-hip ratio (WHR) as measures of anthropometric features in the adult OSA population and controls, utilized in-lab polysomnography or home sleep testing with apnea-hypopnea index (AHI) or respiratory disturbance index (RDI) classification, reported ethnicity/race, and were published in English. Any studies lacking one of these criteria or sufficient data were excluded.

Results: Forty studies with a total of 19,142 subjects were investigated. Comparison of changes between patients with and without OSA showed that OSA patients had a higher BMI [mean difference (MD) 3.12, 95% confidence interval (CI): 2.51-3.73], NC (MD 3.10, 95% CI: 2.70-3.51), WC (MD 9.84, 95% CI: 8.42-11.26) and waist-hip ratio (MD 0.04, 95% CI: 0.03-0.05) than the control subjects. The accumulated time-dependent increase in population with OSA was significantly apparent with all anthropometric features. BMI increased from 2000 (MD 0.50) to 2012 (MD 3.08-3.48) and remained stable afterwards (MD 2.70-3.17), NC increased from 2000 (MD 0.40) to 2013 (MD 3.09) and remained stable afterwards too (MD 3.06-3.21). WC increased from 2000 (MD 2.00) to 2012 (MD 9.37-10.03) and also remained stable afterwards (MD 8.99-9.84). WHR was stable from 2000 to 2004 with an MD of 0.01 and then stable from 2007 onwards with an increased MD of 0.03-0.04. Compared with Caucasian patients, Asian patients had lower obesity relevant variates.

Conclusions: BMI, NC, WC and WHR are associated with OSA in both ethnic groups. Anthropometry for overall and regional obesity could facilitate differentiation of patients with OSA from individuals without OSA by ethnicity.
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http://dx.doi.org/10.21037/jtd-20-1799DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024799PMC
March 2021

In vitro and in vivo degradation of programmed cell death ligand 1 (PD-L1) by a proteolysis targeting chimera (PROTAC).

Bioorg Chem 2021 Mar 19;111:104833. Epub 2021 Mar 19.

The State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Tianjin 300071, PR China.

Immunotherapy via immune checkpoints blockade has aroused the attention of researchers worldwide. Inhibition of the programmed cell death-1 (PD-1)/programmed cell death-ligand 1 (PD-L1) interaction has been one of the most promising immunotherapy strategies. Several neutralizing antibodies targeting this interaction have been developed, which have already achieved considerable clinical success. Additionally, numerous pharmaceutical companies have been committed to develop small molecules which could block the interaction between PD-1 and PD-L1. In this study, a novel PROTAC molecule 21a was developed, and effectively induced the degradation of PD-L1 protein in various malignant cells in a proteasome-dependent manner. Moreover, compound 21a could significantly reduce PD-L1 protein levels of MC-38 cancer cells in vivo, by which promoted the invasion of CD8 T cells and inhibited the growth of MC-38 in vivo. This PROTAC molecule could be used as a novel and alternative strategy for cancer immunotherapy.
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http://dx.doi.org/10.1016/j.bioorg.2021.104833DOI Listing
March 2021

E3 ubiquitin ligase PJA1 regulates lung adenocarcinoma apoptosis and invasion through promoting FOXR2 degradation.

Biochem Biophys Res Commun 2021 Jun 8;556:106-113. Epub 2021 Apr 8.

Department of Oncology, The People's Hospital of Jianyang, No.180 Hospital Road, Jianyang City, Sichuan Province, China.

Among all lung cancer cases, lung adenocarcinoma (LAC) represents nearly 40% and remains the leading cause of cancer deaths worldwide. Although the combination therapy of surgical treatment with radiotherapy, chemotherapy, and immunotherapy, has been used to treat LAC, unfortunately, high recurrence rates and poor survival remain. Therefore, novel prognostic markers and new targets for molecular targeted therapy in LAC is urgently needed. Fork-head box R2 (FOXR2) plays a key role in a wide range of cellular processes, including cellular proliferation, invasion, differentiation, and apoptosis, and it has been reported to be implicated in progression of LAC, thus inhibition of FOXR2 may be a novel targeting therapy for lung cancer. This current study found that E3 ligase PJA1 regulates ubiquitin-mediated degradation of FOXR2 and predicts good outcome of patients with LAC. In addition, it was showed force expression of PJA1 significantly inhibited LAC cells invasion and induced apoptosis in vitro through inactivating Wnt/β-catenin signaling pathway. In short, our findings reveal that PJA1 could be a potential diagnostic and prognostic biomarkers and the PJA1- FOXR2 axis could be served as a promising target for LAC therapy.
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http://dx.doi.org/10.1016/j.bbrc.2021.03.137DOI Listing
June 2021

The Effectiveness of the Supine Position in Managing Inferior Breaks in Rhegmatogenous Retinal Detachment After Vitrectomy with Gas Tamponade.

Int J Gen Med 2021 1;14:1179-1184. Epub 2021 Apr 1.

Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430022, People's Republic of China.

Objective: This study aims to determine whether the supine position is effective for the management of inferior peripheral breaks after pars plana vitrectomy with gas tamponade.

Methods: A total of 29 patients (29 eyes) with acute rhegmatogenous retinal detachment and causative peripheral inferior breaks, located between the four o'clock and eight o'clock positions, underwent pars plana vitrectomy with gas tamponade. These patients maintained a face-up supine position for at least six hours each day for 14 days postoperatively. The alternate lateral position was used for the remaining hours, depending on the distribution of the retinal breaks.

Results: The final retinal reattachment rate was 100%, and the visual acuity improvement rate was 100% postoperatively, with no recurrence during the one-year follow up. No patients suffered from any sight-threatening complications. Of the 16 patients with preoperatively clear lenses, 3 were documented to have a cataract during their three-month postoperative follow up. Four patients were documented to have increased intraocular pressure, which was controllable during the early postoperative days.

Conclusion: Postoperative pars plana vitrectomy and gas tamponade in the supine position is effective for managing primary rhegmatogenous retinal detachment with causative breaks between the four o'clock and eight o'clock positions.
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http://dx.doi.org/10.2147/IJGM.S306006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021250PMC
April 2021

MTA2 triggered R-loop trans-regulates BDH1-mediated β-hydroxybutyrylation and potentiates propagation of hepatocellular carcinoma stem cells.

Signal Transduct Target Ther 2021 Apr 2;6(1):135. Epub 2021 Apr 2.

State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, China.

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http://dx.doi.org/10.1038/s41392-021-00464-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016859PMC
April 2021

Finite Element Analysis of Femoral Neck Fracture Treated with Bidirectional Compression-Limited Sliding Screw.

Med Sci Monit 2021 Mar 30;27:e929163. Epub 2021 Mar 30.

Department of Anatomy, School of Basic Medical Science, Southern Medical University and Guangdong Provincial Key Laboratory of Medical Biomechanics and Academy of Orthopedics of Guangdong Province, Guangzhou, Guangdong, China (mainland).

BACKGROUND The rate of femoral neck shortening after internal fixation for femoral neck fracture is high and this complication reduces the function of the affected lower limb. The aim of this study was to design a bidirectional compression-limited sliding screw (BCLSC) that can achieve a full balance between retaining the sliding pressure of the ends of and maintaining the length of the femoral neck. MATERIAL AND METHODS We constructed a 3-dimensional model of a Pauwels III femoral neck fracture and models of 3 internal fixation methods (3 cannulated screws [3CS], dynamic hip screw [DHS]+CS, and BCLSC) by finite element analysis (FEA).The finite element model simulated the loading of the human body when standing on 1 leg. Displacement and stress distribution of the models were calculated based on an axial stress of 600 N. RESULTS The peak von Mises stress (VMS) values of fracture ends in the 3CS, DHS+CS and BCLSC groups were 94.687 MPa, 26.375 MPa and 45.698 MPa; the peak VMS values of internal fixed stress were 451.53 MPa, 174.45 MPa, and 337.34 MPa; the peak VMS values of the lateral femoral wall were 70.021 MPa, 53.033 MPa, and 20.009 MPa; maximum displacements of the femoral head were 1.4482 mm, 1.3813 mm, and 1.3889 mm; and the internal fixed displacement peaks were 4.1134 mm, 3.91 mm, and 4.1004 mm, respectively. CONCLUSIONS The FEA showed that compared with the CS, the new BCLSC showed better performance in resisting shearing force for Pauwels III femoral neck fracture, with better mechanical properties. These data provide a basis for further experiments and clinical application.
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http://dx.doi.org/10.12659/MSM.929163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019266PMC
March 2021

Collaborative compounding of metal-organic frameworks for dispersive solid-phase extraction HPLC-MS/MS determination of tetracyclines in honey.

Food Chem 2021 Sep 26;355:129411. Epub 2021 Feb 26.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Metal-organic frameworks (MOFs), a sort of dispersive solid-phase extraction (d-SPE) material, has shown considerable prospects in the pretreatment of food, biological and other complex samples. Herein, we developed a method for compounding MOFs for d-SPE and trace determination of tetracyclines (TCs) in honey. When the compounding ratio of MIL-101 (Cr), MIL-100 (Fe) and MIL-53 (Al) was 7:1:2, adsorption-extraction was effective. Followed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), the limits of detection were 0.073-0.435 ng/g and the limits of quantitation ranged from 0.239 to 1.449 ng/g for oxytetracycline, tetracycline, chlortetracycline and doxycycline. The method was applied to four kinds of honey samples with recoveries from 88.1% to 126.2%. The compounding of MOFs provides a strategy for purification and multi-target extraction from complex food matrices by d-SPE.
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http://dx.doi.org/10.1016/j.foodchem.2021.129411DOI Listing
September 2021

Comparison of hybrid membrane aerated biofilm reactor (MABR)/suspended growth and conventional biological nutrient removal processes.

Water Sci Technol 2021 Mar;83(6):1418-1428

Department of Environmental Engineering, University of Michigan, 1351 Beal Avenue, Ann Arbor, MI 48109, USA E-mail:

Mathematical modelling was used to investigate the possibility to use membrane aerated biofilm reactors (MABRs) in a largely anoxic suspended growth bioreactor to produce the nitrate-nitrogen required for heterotrophic denitrification and the growth of denitrifying phosphorus accumulating organisms (DPAOs). The results indicate that such a process can be used to achieve a variety of process objectives. The capture of influent biodegradable organic matter while also achieving significant total inorganic nitrogen (TIN) removal can be achieved with or without use of primary treatment by operation at a relatively short suspended growth solids residence time (SRT). Low effluent TIN concentrations can also be achieved, irrespective of the influent wastewater chemical oxygen demand (COD)/total nitrogen (TN) ratio, with somewhat larger suspended growth SRT. Biological phosphorus and nitrogen removal can also be effectively achieved. Further experimental work is needed to confirm these modelling results.
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http://dx.doi.org/10.2166/wst.2021.062DOI Listing
March 2021

Investigating the Aggregation of Imported Cutaneous Leishmaniasis in Henan, Central China.

Biomed Environ Sci 2021 Mar;34(3):247-249

Henan Key Laboratory of Infectious Disease Microbiology, Henan Center for Disease Control and Prevention, Zhengzhou 450016, Henan, China.

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http://dx.doi.org/10.3967/bes2021.032DOI Listing
March 2021

Bioinformatics-Based Identification of HDAC Inhibitors as Potential Drugs to Target EGFR Wild-Type Non-Small-Cell Lung Cancer.

Front Oncol 2021 8;11:620154. Epub 2021 Mar 8.

Department of Respiratory and Infectious Disease of Geriatrics, The First Hospital of China Medical University, Shenyang, China.

Patients with EGFR-mutant non-small-cell lung cancer (NSCLC) greatly benefit from EGFR-tyrosine kinase inhibitors (EGFR-TKIs) while the prognosis of patients who lack EGFR-sensitive mutations (EGFR wild type, EGFR-WT) remains poor due to a lack of effective therapeutic strategies. There is an urgent need to explore the key genes that affect the prognosis and develop potentially effective drugs in EGFR-WT NSCLC patients. In this study, we clustered functional modules related to the survival traits of EGFR-WT patients using weighted gene co-expression network analysis (WGCNA). We used these data to establish a two-gene prognostic signature based on the expression of CYP11B1 and DNALI1 by combining the least absolute shrinkage and selection operator (LASSO) algorithms and Cox proportional hazards regression analysis. Following the calculation of risk score (RS) based on the two-gene signature, patients with high RSs showed a worse prognosis. We further explored targeted drugs that could be effective in patients with a high RS by the connectivity map (CMap). Surprisingly, multiple HDAC inhibitors (HDACis) such as trichostatin A (TSA) and vorinostat (SAHA) that may have efficacy were identified. Also, we proved that HDACis could inhibit the proliferation and metastasis of NSCLC cells . Taken together, our study identified prognostic biomarkers for patients with EGFR-WT NSCLC and confirmed a novel potential role for HDACis in the clinical management of EGFR-WT patients.
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http://dx.doi.org/10.3389/fonc.2021.620154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982742PMC
March 2021