Publications by authors named "Yang Cao"

1,746 Publications

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Quality of Life After Percutaneous Coronary Intervention in No-Touch Saphenous Vein Grafts is Significantly Better Than in Conventional Vein Grafts.

Braz J Cardiovasc Surg 2022 Aug 16;37(4):430-438. Epub 2022 Aug 16.

University Health Care Research Centre, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

Objective: To compare health-related quality of life (HRQoL) of patients primarily treated with a no-touch saphenous vein graft with that of patients who received a conventional graft.

Methods: The study included all individuals treated with a percutaneous coronary intervention (PCI) on a saphenous vein graft (SVG) between January 2006 and June 2020. The RAND-36 health survey was used to assess HRQoL. The Mann-Whitney U test was used to test differences in HRQoL between the two groups. Effect size was estimated via Cohen's d. The average treatment effect between the groups was tested by propensity score matching (PSM).

Results: Of the 346 patients treated with a PCI in a stenosed or occluded SVG, 165 responded to RAND-36 (no-touch: n=48; conventional: n=117). Patients with a no-touch graft reported better mean values on seven of the eight health survey domains. Statistically significant differences were observed for four of the domains, all in favour of the no-touch group. The effect size estimates indicated a small difference for five domains, with the highest values (>0.40) seen for the general health and energy/fatigue domains. PSM confirmed a statistically significant difference for the physical functioning and general health domains.

Conclusion: At a mean follow-up of 5.4 years, patients who received a PCI in no-touch vein grafts showed significantly better HRQoL than those who received a PCI in conventional vein grafts.
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http://dx.doi.org/10.21470/1678-9741-2021-0576DOI Listing
August 2022

Functional and structural characteristics of HLA-B*13:01-mediated specific T cells reaction in dapsone-induced drug hypersensitivity.

J Biomed Sci 2022 Aug 13;29(1):58. Epub 2022 Aug 13.

Department of Mycobacterium, Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Institute of Dermatology & Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, China.

Background: Severe cutaneous adverse drug reactions (SCARs) are a group of serious clinical conditions caused by immune reaction to certain drugs. The allelic variance of human leukocyte antigens of HLA-B*13:01 has been strongly associated with hypersensitivities induced by dapsone (DDS). T-cell receptor mediated activation of cytotoxic T lymphocytes (CTLs) has also been suggested to play an essential role in pathogenesis of SCARs. However, HLA-B*13:01-DDS-TCR immune synapse that plays role in drug-induced hypersensitivity syndrome (DIHS) associated T cells activation remains uncharacterized.

Methods: To investigate the molecular mechanisms for HLA-B*13:01 in the pathogenesis of Dapsone-induced drug hypersensitivity (DDS-DIHS), we performed crystallization and expanded drug-specific CTLs to analyze the pathological role of DDS-DIHS.

Results: Results showed the crystal structure of HLA-B*13:01-beta-2-microglobulin (β2M) complex at 1.5 Å resolution and performed mutation assays demonstrating that I118 or I119, and R121 of HLA-B*13:01 were the key residues that mediate the binding of DDS. Subsequent single-cell TCR and RNA sequencing indicated that TCRs composed of paired TRAV12-3/TRBV28 clonotype with shared CDR3 region specifically recognize HLA-B*13:01-DDS complex to trigger inflammatory cytokines associated with DDS-DIHS.

Conclusion: Our study identified the novel p-i-HLA/TCR as the model of interaction between HLA-B*13:01, DDS and the clonotype-specific TCR in DDS-DIHS.
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http://dx.doi.org/10.1186/s12929-022-00845-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375929PMC
August 2022

Construction of heterotrophic-sulfur autotrophic integrated fluidized bed reactor for simultaneous and efficient removal of compound pollution of perchlorate and nitrate in water.

Chemosphere 2022 Aug 10;307(Pt 3):135944. Epub 2022 Aug 10.

College of Environmental Engineering, Henan University of Technology, Zhengzhou, Henan, 450001, China.

A heterotrophic sulfur autotrophic integrated fluidized bed reactor was established for simultaneous and efficient removal of ClO and NO from water. The optimum operating conditions forecasted through the response surface method (RSM) were the hydraulic retention time (HRT) of 0.50 h, the influent acetate (CHCOO) concentration of 55 mg/L and the reflux ratio of 14, contributing to ClO and NO removal of 98.99% and 99.96%, respectively, without secondary pollution caused by residual carbon (NPOC <3.89 mg/L). Meanwhile, the effluent pH fluctuated in a range of 6.70-8.02 and sulfur-containing by-products (i.e., SO and S) could be controlled by adjusting operation conditions throughout the experimental stage. The increase of the influent CHCOO concentration reduced the load borne by autotrophic reduction process and further reduced SO production. Shortening HRT, increasing the influent CHCOO concentration and decreasing the reflux ratio could all reduce alkalinity consumption. Shortening HRT and decreasing the reflux ratio could shorten contact time between sulfur and water and thus inhibit S disproportionation. High-throughput sequencing result showed that Proteobacteria and Chlorobi were the dominant bacteria. Sulfurovum, Sulfuricurvum and Ignavibacterium were the major heterotrophic denitrifying bacteria (DB)/perchlorate reducing bacteria (PRB), Ferritrophicum and Geothrix were DB, and Chlorobaculum was S disproportionation bacteria.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135944DOI Listing
August 2022

TMUB1 is an endoplasmic reticulum-resident escortase that promotes the p97-mediated extraction of membrane proteins for degradation.

Mol Cell 2022 Aug 10. Epub 2022 Aug 10.

Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address:

Membrane protein clients of endoplasmic reticulum (ER)-associated degradation must be retrotranslocated from the ER membrane by the AAA-ATPase p97 for proteasomal degradation. Before direct engagement with p97, client transmembrane domains (TMDs) that have partially or fully crossed the membrane must be constantly shielded to avoid non-native interactions. How client TMDs are seamlessly escorted from the membrane to p97 is unknown. Here, we identified ER-anchored TMUB1 as a TMD-specific escortase. TMUB1 interacts with the TMD of clients within the membrane and holds ∼10-14 residues of a hydrophobic sequence that is exposed out of membrane, using its transmembrane and cytosolic regions, respectively. The ubiquitin-like domain of TMUB1 recruits p97, which can pull client TMDs from bound TMUB1 into the cytosol. The disruption of TMUB1 escortase activity impairs retrotranslocation and stabilizes retrotranslocating intermediates of client proteins within the ER membrane. Thus, TMUB1 promotes TMD segregation by safeguarding the TMD movement from the membrane to p97.
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http://dx.doi.org/10.1016/j.molcel.2022.07.006DOI Listing
August 2022

Unmet Needs and Associated Factors among Community-living Older People with Disability in China: 2005-2014.

J Aging Soc Policy 2022 Aug 11:1-19. Epub 2022 Aug 11.

Institute of Gerontology, Renmin University, Beijing, P.R. China.

Based on the Chinese Longitudinal Healthy Longevity Survey from 2005 to 2014, this study estimated the prevalence and examined risk factors of under-met needs and completely unmet needs for assistance in activities of daily living (ADLs) among community-living older people with disability in China. As of 2014, over 50% of community-living Chinese elders with disability experienced under-met needs, and nearly 5% had completely unmet needs. From 2005 to 2014, the proportion with completely unmet needs doubled for all disabled elders. Significant risk factors of under-met needs included lower per capita annual household income, more ADL limitations, living alone, and fewer living children, and those of completely unmet needs included less ADL limitations and living alone. More policy attention should be paid to address the gap between long-term care services for older persons with severe disability and supportive services for those who are relatively healthy, toward ultimately establishing a care continuum for the elderly at all stages of their life course. In addition, family care for elders with severe functional impairments should be supplemented by professional long-term care services to best meet their needs.
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http://dx.doi.org/10.1080/08959420.2022.2110806DOI Listing
August 2022

photoacoustic image-guided tumor photothermal therapy and real-time temperature monitoring using a core-shell [email protected] nanohybrid.

Nanoscale 2022 Aug 10. Epub 2022 Aug 10.

Chongqing Key Laboratory of Ultrasound Molecular Imaging, Institute of Ultrasound Imaging, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.

Near-infrared (NIR) laser triggered theranostic platforms are increasingly used in clinical nanomedicine applications. In this work, a core-shell composite consisting of polypyrrole (PPy) coated copper sulfide (CuS) nanospheres with high photothermal efficiency and good photostability has been fabricated a facile interfacial polymerization. The [email protected] nanohybrid had a hydrodynamic diameter of 58.5 nm with a CuS core and PPy shell and exhibited strong optical absorption and photon-to-heat conversion in the NIR region, leading to a sufficient photohyperthermic effect under irradiation with a 808 nm continuous wave laser. studies showed that the [email protected] nanohybrids produced significant photoacoustic signals and exhibited remarkable photothermal therapeutic efficacy. Furthermore, the core-shell composites exhibited improved temperature elevation and photostability. The temperature-induced changes can be detected and monitored using photoacoustic imaging, thus allowing the control of the thermal dose while minimizing photothermal damage to surrounding healthy tissues. In summary, this study demonstrates that this novel platform could potentially be used for photoacoustic image-guided photothermal therapy and real-time temperature monitoring in cancer theranostics.
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http://dx.doi.org/10.1039/d2nr02848dDOI Listing
August 2022

Common Bile Duct Stones Management: A Network Meta-analysis.

J Trauma Acute Care Surg 2022 Aug 5. Epub 2022 Aug 5.

Background: Timely management is critical for treating symptomatic common bile duct (CBD) stones; however, a single optimal management strategy has yet to be defined in the acute care setting. Consequently, this systematic review and network meta-analysis, comparing 1-stage (CBD exploration or intraoperative ERCP with simultaneous cholecystectomy) and 2-stage (pre- or post-cholecystectomy ERCP) procedures, was undertaken with the main outcomes of interest being post-procedural complications and hospital length of stay (LOS).

Methods: PubMed, SCOPUS, MEDLINE, Embase, and Cochrane Central Register of Controlled. Trials were methodically queried for articles from 2010 to 2021. The search terms were a combination of medical subject headings terms and the subsequent terms: Gallstone, Common Bile Duct (stone), Choledocholithiasis, Cholecystitis, Endoscopic Retrograde Cholangiography/ERCP, Common Bile Duct Exploration, Intra-, Pre-, Peri-, and Post-operative Endoscopic Retrograde Cholangiography, Stone Extraction, One-stage- & Two-stage-procedure. Studies that compared ≥2 procedures were included, whereas studies not recording complications (bile leak, hemorrhage, pancreatitis, perforation, intraabdominal infections, and other infections) or LOS were excluded. A network meta-analysis was conducted to compare the four different approaches for managing CBD stones.

Results: A total of 16 studies (8,644 participants) addressing the LOS and 41 studies (19,756 participants) addressing post-procedural complications were included in the analysis. The 1-stage approaches were associated with a decrease in LOS compared to the 2-stage approaches. CBD exploration demonstrated a lower overall risk of complications compared to preoperative ERCP, but there were no differences in the overall risk of complications in the remaining comparisons. However, differences in specific post-procedural complications were detected between the four different approaches managing CBD stones.

Conclusion: This network meta-analysis suggests that both laparoscopic CBDE and intraoperative ERCP have equally good outcomes and provide a preferable single-anesthesia patient pathway with a shorter overall length of hospital stay compared to the 2-stage approaches.

Level Of Evidence: Level II, Systemic review and network meta-analysis.
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http://dx.doi.org/10.1097/TA.0000000000003755DOI Listing
August 2022

Relationship between unmet needs for assistance and healthy aging among disabled older adults in China.

Front Public Health 2022 20;10:914313. Epub 2022 Jul 20.

Department of Labor and Social Security, School of Public Administration, Sichuan University, Chengdu, China.

Background: Although there is a growing consensus around the world that long-term care services and supports are important to help the aged population with disabilities achieve healthy aging, a misallocation of care resources and inefficiency in care delivery still exist in China. The absence or inadequate provision of long-term care services and supports among older adults with disabilities results in a range of adverse health consequences. However, the negative influence of unmet needs for assistance on healthy aging, based on functional perspectives including physiological, psychological, and societal domains, has been underestimated. This study aimed to measure healthy aging based on a person-centered approach and examine the relationship between unmet needs for assistance and healthy aging among older adults with disabilities in China.

Methods: Based on the data from the Chinese Longitudinal Healthy Longevity Survey 2018, we used the latent profile analysis with three indicators to uncover distinctive types of older adults experiencing distinct levels of healthy aging, and applied the ordered logit regression to analyze the correlation between unmet needs for assistance and different levels of healthy aging. To further address the endogeneity bias, the robust test was conducted by the two-stage least-squares instrumental variable estimation and the conditional mixed process instrumental variable estimation.

Results: Three ordered latent classes were identified: a low level of healthy aging (42.83%), a middle level of healthy aging (47.27%), and a high level of healthy aging (9.90%). Disabled older adults with unmet needs had a lower probability of achieving the higher level of healthy aging ( = 0.57, = 0.04, = 0.48-0.66, < 0.001).

Conclusions: This study highlights the need to increase awareness among gerontological practitioners with respect to long-term care services and supports for disabled older adults as a potential for enhancing their healthy aging, and that unmet needs could be a basis for risk assessment and a means for determining the efficacy of long-term care interventions on maintaining health.
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http://dx.doi.org/10.3389/fpubh.2022.914313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350852PMC
August 2022

A simultaneous electroencephalography and eye-tracking dataset in elite athletes during alertness and concentration tasks.

Sci Data 2022 08 2;9(1):465. Epub 2022 Aug 2.

Human Phenome Institute, State Key Laboratory of Medical Neurobiology and Ministry of Education Frontiers Center for Brain Science, School of Life Science, Research Institute of Intelligent Complex Systems, and Institute of Science and Technology for Brain-Inspired Intelligence, Fudan University, Shanghai, China.

The dataset of simultaneous 64-channel electroencephalography (EEG) and high-speed eye-tracking (ET) recordings was collected from 31 professional athletes and 43 college students during alertness behavior task (ABT) and concentration cognitive task (CCT). The CCT experiment lasting 1-2 hours included five sessions for groups of the Shooting, Archery and Modern Pentathlon elite athletes and the controls. Concentration targets included shooting target and combination target with or without 24 different directions of visual distractors and 2 types of music distractors. Meditation and Schulte Grid trainings were done as interventions. Analysis of the dataset aimed to extract effective biological markers of eye movement and EEG that can assess the concentration level of talented athletes compared with same-aged controls. Moreover, this dataset is useful for the research of related visual brain-computer interfaces.
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http://dx.doi.org/10.1038/s41597-022-01575-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345900PMC
August 2022

N-cadherin mimetic hydrogel enhances MSC chondrogenesis through cell metabolism.

Acta Biomater 2022 Jul 30. Epub 2022 Jul 30.

Department of Orthopaedics, Tongji Medical College, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China. Electronic address:

Mesenchymal stem cells (MSCs) are ideal candidates for tissue engineering and regenerative medicine because of their proliferative capacity and differentiation potential. However, the hypertrophic phenotype occurring in late MSCs chondrogenic differentiation severely limits their clinical translation. While hypertrophy inhibition strategies have been explored, the role of cell metabolism in MSCs chondrogenesis has rarely been studied. In this study, we found that hypertrophy occurred in the late stage of MSCs chondrogenesis with increased fatty acid oxidation (FAO) and decreased glycolysis, as well as cell-cell junctions impairment. Therefore, a N-cadherin mimetic hydrogel was developed to enhance cell-cell junctions via N-cadherin mimetic peptides and high seeding density. The N-cadherin mimetic hydrogel attenuated hypertrophy through regulating glycolysis and FAO. The regulation of cell-cell junctions mechanotransduction on cell metabolism was partly mediated by Hif-1α. In addition, 2D and 3D culture of N-cadherin mimetic hydrogel had similar functions on N-cadherin expression and chondrogenesis in MSCs. Our study is the first to reveal that metabolic remodeling induced hypertrophy during MSCs chondrogenesis, and indicate the effect of N-cadherin mimetic hydrogel on hypertrophy inhibition of MSCs. STATEMENT OF SIGNIFICANCE: The development of hypertrophy during MSCs chondrogenesis severely limits its clinical translation. Various strategies have been explored to inhibit hypertrophy by chemical and/or mechanical stimulation. However, the role of cell metabolism in MSCs chondrogenesis has rarely been studied. In this study, we developed an RNA sequencing at day 0, 7, and 21 of MSCs chondrogenesis to clarify the mechanisms that mediate hypertrophy. We found that hypertrophy occurred in the late stage of MSCs chondrogenesis with increased FAO and decreased glycolysis, as well as impaired cell-cell junctions. We also found that N-cadherin mimetic hydrogel attenuated hypertrophy and enhanced chondrogenesis through regulating glycolysis and FAO. Our finding provides new insights into the application of MSCs in tissue engineering and regenerative medicine.
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http://dx.doi.org/10.1016/j.actbio.2022.07.050DOI Listing
July 2022

Nanoparticles in ocular applications and their potential toxicity.

Front Mol Biosci 2022 15;9:931759. Epub 2022 Jul 15.

Southwest Hospital/Southwest Eye Hospital, Third Military Medical University (Army Medical University), Chongqing, China.

Nanotechnology has been developed rapidly in recent decades and widely applied in ocular disease therapy. Nano-drug delivery systems overcome the bottlenecks of current ophthalmic drug delivery and are characterized with strong biocompatibility, stability, efficiency, sustainability, controllability, and few side effects. Nanoparticles have been identified as a promising and generally safe ophthalmic drug-delivery system based on the toxicity assessment in animals. Previous studies have found that common nanoparticles can be toxic to the cornea, conjunctiva, and retina under certain conditions. Because of the species differences between humans and animals, advanced cell culture techniques, such as human organoids, can mimic the human organism to a certain extent, bringing nanoparticle toxicity assessment to a new stage. This review summarizes the advanced application of nanoparticles in ocular drug delivery and the potential toxicity, as well as some of the current challenges and future opportunities in nanotoxicological evaluation.
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http://dx.doi.org/10.3389/fmolb.2022.931759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334523PMC
July 2022

Process water recirculation for catalytic hydrothermal carbonization of anaerobic digestate: Water-Energy-Nutrient Nexus.

Bioresour Technol 2022 Jul 26;361:127694. Epub 2022 Jul 26.

Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China; Research Institute for Future Food, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China. Electronic address:

The process water (PW) from acid-catalyzed hydrothermal carbonization (HTC) is still an environmental burden due to the enriched organics, nutrients, and salts. This study proposed a novel strategy to valorize food waste digestate into multifunctional hydrochar by recirculating the PW in the HCl-catalyzed HTC process. The produced multifunctional hydrochar could be utilized as a high-quality solid fuel with HHV of 27.9 MJ kg (hydrochar without PW recirculation) and a slow-release fertilizer by converting the complex Ca and P compounds from the food waste digestate into a Ca-P deposit (hydroxyapatite) with more than a 93 % P recovery rate (hydrochar with PW recirculation). Adding fresh HCl in the HTC PW recirculation system only displayed a marginal catalytic impact on the hydrochar properties after two cycles of recirculation. This study demonstrated the importance of inherent Ca in the feedstocks and the dual role of HCl in the HTC with PW recirculation.
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http://dx.doi.org/10.1016/j.biortech.2022.127694DOI Listing
July 2022

Effects of the interaction of Notch and TLR4 pathways on inflammation and heart function in septic heart.

Open Life Sci 2022 13;17(1):744-755. Epub 2022 Jul 13.

Intensive Care Unit, Emergency Trauma Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang, China.

We investigated the role of the interaction between the Notch and Toll-like receptor 4 (TLR4) pathways in septic myocardial injury. The sepsis model was induced in rats with lipopolysaccharide (LPS). Rats were divided into control, LPS, LPS + TAK242 ((6)-6-[-(2-chloro-4-fluorophenyl)sulfamoyl]cyclohex-1-ene-1-carboxylate) and LPS + DAPT (-[-(3,5-difluorophenacetyl)-l-alanyl]--phenylglycinetbutylester) groups. Heart function was evaluated with a Cardiac Doppler ultrasound. Myocardial morphological changes were detected by hematoxylin-eosin staining (H&E). Apoptosis was assessed by a TUNEL assay. The mRNA and protein levels were detected with real-time PCR, Western blot, and immunohistochemistry analysis. We found that heart function in the LPS + TAK242 group was significantly improved, but not in the LPS + DAPT group. LPS + TAK242 had a lower level of degeneration and necrosis of cardiomyocytes and inflammatory cell infiltration, as well as lower apoptosis and caspase-3 expression than the LPS group. Compared with the LPS group, the inflammatory cell infiltration was reduced in the LPS + DAPT group, while the degeneration and necrosis of cardiomyocytes were not obviously improved. Additionally, the expression levels of tumor necrosis factor-α and Interleukin-6, the protein contents of Notch intracellular domain and Hes1, and the P65 nuclear factor kappa-B (NF-κB) to P-P65 NF-κB ratio in LPS + TAK242 group and LPS + DAPT group were significantly lower than those in LPS group. Conclusively, the interaction between TLR4 and Notch signaling pathways enhances the inflammatory response in the septic heart by activating NF-κB. Blocking the TLR4 pathway with TAK242 can improve heart dysfunction and myocardial damage in sepsis, while blocking the Notch pathway with DAPT cannot effectively prevent heart dysfunction and myocardial damage in sepsis.
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http://dx.doi.org/10.1515/biol-2022-0076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281592PMC
July 2022

The Relationships between Caregivers' Concern about Child Weight and Their Non-Responsive Feeding Practices: A Systematic Review and Meta-Analysis.

Nutrients 2022 Jul 14;14(14). Epub 2022 Jul 14.

Clinical Epidemiology and Biostatistics, School of Medical Sciences, Örebro University, 70182 Örebro, Sweden.

Background: It is unclear whether caregivers' concern about child weight impacts their non-responsive feeding practices. This systematic review aimed to examine their relationships.

Methods: A systematic search of PubMed, Embase, PsycINFO, Web of Science core collection, CINAHL and grey literature was conducted from inception to March 2022, following PRISMA guidelines. Data synthesis was performed using a semi-quantitative approach and a meta-analysis.

Results: A total of 35 studies with 22,933 respondents were included in the review for semi-quantitative analyses. Thirty-four studies examined 52 associations between concern about child weight and restriction with 40 statistically significant associations being observed. A total of 34 relationships between concern about child weight and pressure to eat were investigated, with 12 being statistically significant. The pooled regression coefficients (β) demonstrated that caregivers' concern about child overweight was positively associated with restriction (β = 0.22; 95%CI: 0.12, 0.31), negatively associated with use of food as a reward (β = -0.06; 95%CI: -0.11, -0.01), and not statistically associated with pressure to eat (β = -0.05; 95%CI: -0.13, 0.04). The pooled odds ratios (ORs) indicated that caregivers who were concerned about child overweight were found to use restrictive feeding more often (OR = 2.34; 95%CI: 1.69, 3.23), while less frequently adopting pressure to eat (OR = 0.76; 95%CI: 0.59, 0.98) compared with those without concerns. The results also showed that caregivers who were concerned about child underweight were more likely to force their children to eat (OR = 1.83; 95%CI: 1.44, 2.33) than those without concerns.

Conclusion: Caregivers' concern about child weight may be an important risk factor for non-responsive feeding practices. Thus, interventions are needed to focus on managing and relieving caregivers' excessive concern about child weight, especially overweight, which may optimize feeding practices and subsequently contribute to child health.
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http://dx.doi.org/10.3390/nu14142885DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323971PMC
July 2022

Proteomic and parallel reaction monitoring approaches to evaluate biomarkers of mutton tenderness.

Food Chem 2022 Jul 19;397:133746. Epub 2022 Jul 19.

Institute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. Electronic address:

Intensive fattening usually results in the changes of meat quality. Tenderness is a central attribute for mutton sensory qualities and consumers' choice. Here, we reported that intensive fattening mutton was more tender than that of traditionally raised sheep. By proteomic approach, we found 49 differentially expressed proteins in longissimus dorsi muscle. After bioinformatics analysis, 5 cytoskeletal proteins, 3 protein binding proteins and 7 metabolic enzymes were identified as potential biomarkers for mutton tenderness. Finally, we verified the expression of these abundant proteins by parallel reaction monitoring (PRM). Collectively, our results reveal that the mutton of sheep raised by intensive fattening is more tender than that of traditionally raised sheep. Myosin-2, myosin-13, vimentin, carbonic anhydrase, carbonic anhydrase-2, Glutathione S-transferase and Microtubule-associated protein 4 isoform X1 can be candidate biomarkers for mutton tenderness. Our data also indicate a central role of cytoskeletal proteins and metabolic enzymes in determining mutton tenderness.
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http://dx.doi.org/10.1016/j.foodchem.2022.133746DOI Listing
July 2022

Room-temperature epitaxial welding of 3D and 2D perovskites.

Nat Mater 2022 Jul 25. Epub 2022 Jul 25.

Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech), Nanjing, China.

Formation of epitaxial heterostructures via post-growth self-assembly is important in the design and preparation of functional hybrid systems combining unique properties of the constituents. This is particularly attractive for the construction of metal halide perovskite heterostructures, since their conventional solution synthesis usually leads to non-uniformity in composition, crystal phase and dimensionality. Herein, we demonstrate that a series of two-dimensional and three-dimensional perovskites of different composition and crystal phase can form epitaxial heterostructures through a ligand-assisted welding process at room temperature. Using the CsPbBr/PEAPbBr heterostructure as a demonstration, in addition to the effective charge and energy transfer across the epitaxial interface, localized lattice strain was observed at the interface, which was extended to the top layer of the two-dimensional perovskite, leading to multiple new sub-bandgap emissions at low temperature. Given the versatility of our strategy, unlimited hybrid systems are anticipated, yielding composition-, interface- and/or orientation-dependent properties.
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http://dx.doi.org/10.1038/s41563-022-01311-4DOI Listing
July 2022

Role of gut microbe-derived metabolites in cardiometabolic diseases: Systems based approach.

Mol Metab 2022 Jul 21;64:101557. Epub 2022 Jul 21.

Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA.

Background: The gut microbiome influences host physiology and cardiometabolic diseases by interacting directly with intestinal cells or by producing molecules that enter the host circulation. Given the large number of microbial species present in the gut and the numerous factors that influence gut bacterial composition, it has been challenging to understand the underlying biological mechanisms that modulate risk of cardiometabolic disease.

Scope Of The Review: Here we discuss a systems-based approach that involves simultaneously examining individuals in populations for gut microbiome composition, molecular traits using "omics" technologies, such as circulating metabolites quantified by mass spectrometry, and clinical traits. We summarize findings from landmark studies using this approach and discuss future applications.

Major Conclusions: Population-based integrative approaches have identified a large number of microbe-derived or microbe-modified metabolites that are associated with cardiometabolic traits. The knowledge gained from these studies provide new opportunities for understanding the mechanisms involved in gut microbiome-host interactions and may have potentially important implications for developing novel therapeutic approaches.
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http://dx.doi.org/10.1016/j.molmet.2022.101557DOI Listing
July 2022

Prospective study of a new endoscopic duodenal-jejunal bypass sleeve in obese patients with non-alcoholic fatty liver disease (with video).

Dig Endosc 2022 Jul 23. Epub 2022 Jul 23.

Department of Gastroenterology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Objectives: To evaluate the safety and efficacy of a new endoscopic duodenal-jejunal bypass sleeve (DJBS) in obese patients with non-alcoholic fatty liver disease (NAFLD), while in situ for 3 months, and at 6 months post-explantation.

Methods: Patients with obesity and NAFLD were enrolled in this single-center, prospective study, wherein TONGEE® DJBS was implanted for 3 months. Primary outcomes were weight loss and changes in hepatic steatosis. Secondary outcomes included changes in liver enzymes, glycemic control, and lipid profile and device safety.

Results: Twenty-six patients (age: 35.2 ± 7.2 years; 61.5% women) underwent DJBS implantation. At 3 months, body weight change from baseline was -8.0 ± 3.6 kg (P < 0.001), corresponding to 8.9 ± 4.0% of total body weight. Hepatic steatosis significantly improved based on controlled attenuation parameter, hepatic steatosis index, and fatty liver index (P < 0.001). Liver enzymes, insulin resistance, and metabolic parameters were also improved. At 6 months post-explantation, weight loss and improvements in hepatic steatosis and liver enzyme levels remained statistically significant. Only one patient had a serious adverse event, namely, upper gastrointestinal hemorrhage.

Conclusions: Three-month TONGEE® DJBS implantation resulted in significant weight loss and improvement in hepatic steatosis, liver enzymes, insulin resistance, and metabolic parameters in obese patients with NAFLD. Randomized controlled trials are required to further elucidate these initial findings.
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http://dx.doi.org/10.1111/den.14409DOI Listing
July 2022

Targeted Therapy of Lung Adenocarcinoma by the Nanoplatform Based on Milk Exosomes Loaded with Paclitaxel.

J Biomed Nanotechnol 2022 Apr;18(4):1075-1083

School of Medicine, Tsinghua University, Beijing, 100084, China.

Lung cancer is the most common cancer throughout the world. Currently, most lung cancer therapies are still limited by serious side effects caused. This paper reports a biocompatible drug delivery system that utilizes milk-derived exosomes to deliver paclitaxel to treat lung adenocarcinoma. First, milk-derived exosomes were modified with integrin ₃, -binding peptide iRGD so that they could successfully target lung adenocarcinoma cells. Then, iRGD modified exosomes were loaded with paclitaxel (PAC) via electroporation and used for tumor therapy. These modified exosomes proved effective in killing lung adenocarcinoma cells, and the exosome-based nanoplatform showed no obvious toxicity to normal cells. Further more, the exosome-based nanoplatform could effectively penetrate the interior of the 3D tumor sphere, reaching more tumor cells and demonstrating that it is a promising tool for lung adenocarcinoma therapy.
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http://dx.doi.org/10.1166/jbn.2022.3278DOI Listing
April 2022

One-year follow-up study after patients with severe COVID-19 received human umbilical cord mesenchymal stem cells treatment.

Stem Cell Res Ther 2022 07 16;13(1):321. Epub 2022 Jul 16.

Department of Pulmonary and Critical Care Medicine, the Second Affiliated Hospital of Nanjing Medical University, No. 121 Jiangjiayuan Rd, Gulou District, Nanjing, 210011, Jiangsu, China.

Background: The novel coronavirus is still mutating, and the pandemic continues. Meanwhile, many COVID-19 survivors have residual postinfection clinical manifestations. Human umbilical cord mesenchymal stem cells (hUC-MSCs) have been shown to be effective in the early stages of COVID-19.

Objectives: The aim of this study was to investigate long-term safety and efficacy of treatment in patients with severe COVID-19 patients who had received hUC-MSCs therapy.

Methods: Twenty-five discharged patients who had severe COVID-19 (including the standard treatment group and the standard treatment plus hUC-MSCs group) were enrolled in a 1-year follow-up. The assessment considered adverse effects (including effects on liver and kidney function, coagulation, ECG, tumor marker, and so on), pulmonary function, St George's Respiratory Questionnaire (SGRQ), postinfection sequelae and serum concentration of Krebs von den Lungen-6 (KL-6), malondialdehyde (MDA), HS, carnitine, and N-6 long-chain polyunsaturated fatty acids (N-6 LC-PUFAs).

Measurements And Main Results: Pulmonary ventilation function had significantly improved at the 1-year follow-up in both the hUC-MSCs group and the control group compared with the 3-month follow-up (P < 0.01). Fatigue (60% [15/25]) remained the most common symptom at the 1-year follow-up. The rate of fatigue relief was significantly reduced in the hUC-MSCs group (25% [2/8]) compared to the control group (76.5% [13/17]) (P = 0.028). The level of KL-6 was significantly lower in the hUC-MSCs group (2585.5 ± 186.5 U/ml) than in the control group (3120.7 ± 158.3 U/ml) (P < 0.001). Compared with the control group, the hUC-MSCs group had a lower level of MDA (9.27 ± 0.54 vs. 9.91 ± 0.72 nmol/ml, P = 0.036). No obvious adverse effects were observed in the hUC-MSCs treatment group at 1 year after discharge.

Conclusions: Intravenous transplantation of hUC-MSCs was a safe approach in the long term in the treatment of patients with severe COVID-19. In addition, hUC-MSCs had a positive effect on postinfection sequelae in COVID-19 survivors.

Trial Registration: Chinese Clinical Trial Registration; ChiCTR2000031494; Registered 02 April 2020-Retrospectively registered, http://www.medresman.org.
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http://dx.doi.org/10.1186/s13287-022-02972-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288258PMC
July 2022

Correction: Comparative effectiveness of aerobic exercise versus Yi Jin Jing on ovarian function in young overweight/obese women with polycystic ovary syndrome: study protocol for a randomized controlled trial.

Trials 2022 Jul 15;23(1):564. Epub 2022 Jul 15.

Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 110 Ganhe Road, Shanghai, 200437, China.

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http://dx.doi.org/10.1186/s13063-022-06547-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288064PMC
July 2022

The snapshot audit methodology: design, implementation and analysis of prospective observational cohort studies in surgery.

Eur J Trauma Emerg Surg 2022 Jul 15. Epub 2022 Jul 15.

Division of Trauma and Emergency Surgery, Orebro University Hospital and Faculty of School of Medical Sciences, Orebro University, 702 81, Orebro, Sweden.

Purpose: For some surgical conditionns and scientific questions, the "real world" effectiveness of surgical patient care may be better explored using a multi-institutional time-bound observational cohort assessment approach (termed a "snapshot audit") than by retrospective review of administrative datasets or by prospective randomized control trials. We discuss when this might be the case, and present the key features of developing, deploying, and assessing snapshot audit outcomes data.

Methods: A narrative review of snapshot audit methodology was generated using the Scale for the Assessment of Narrative Review Articles (SANRA) guideline. Manuscripts were selected from domains including: audit design and deployment, statistical analysis, surgical therapy and technique, surgical outcomes, diagnostic testing, critical care management, concomitant non-surgical disease, implementation science, and guideline compliance.

Results: Snapshot audits all conform to a similar structure: being time-bound, non-interventional, and multi-institutional. A successful diverse steering committee will leverage expertise that includes clinical care and data science, coupled with librarian services. Pre-published protocols (with specified aims and analyses) greatly helps site recruitment. Mentored trainee involvement at collaborating sites should be encouraged through manuscript contributorship. Current funding principally flows from medical professional organizations.

Conclusion: The snapshot audit approach to assessing current care provides insights into care delivery, outcomes, and guideline compliance while generating testable hypotheses.
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http://dx.doi.org/10.1007/s00068-022-02045-3DOI Listing
July 2022

Hierarchical Natural Pollen Cell-Derived Composite Sorbents for Efficient Atmospheric Water Harvesting.

ACS Appl Mater Interfaces 2022 Jul 15. Epub 2022 Jul 15.

State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, P. R. China.

Freshwater scarcity is a critical challenge threatening human survival especially due to poverty and arid and off-grid regions. Sorption-based atmospheric water harvesting (AWH) has emerged as a promising strategy for clean water production. However, most of the high-capacity sorbents are limited by the poor sorption/desorption kinetics and uncontrollable liquid leakage problem. Inspired by the plant transpiration process, we develop an environmentally friendly [email protected] cell-polypyrrole ([email protected]) composite sorbent by confining the LiCl hygroscopic agent in the cages of the PC-PPy. The composite sorbent exhibits much improved sorption/desorption kinetics owing to the hydrophilicity of the hierarchical porous structure of the pollen cells, which provides abundant water sorption active sites and diffusion pathways and forms a concave meniscus on cell skeletons to maximize the thermal utilization efficiency. Moreover, the big cavities of the PC-PPy cages can serve as a water reservoir to reduce liquid leakage. As a result, the sorbent can capture atmospheric water to 85% of its own weight under 60% relative humidity (RH) within 2 h and rapidly release the water within 1 h under weak light irradiation of 0.8 sun. As a proof-of-concept demonstration, the fabricated AWH device can absorb 1.55 g/g at night and collect 1.53 g/g of water in 1-day outdoor operation, and the collected water can meet the drinking water standards defined by the World Health Organization (WHO) and Environmental Protection Agency (EPA).
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http://dx.doi.org/10.1021/acsami.2c04845DOI Listing
July 2022

Synergistic Poly(lactic acid) Antibacterial Surface Combining Superhydrophobicity for Antiadhesion and Chlorophyll for Photodynamic Therapy.

Langmuir 2022 Jul 15;38(29):8987-8998. Epub 2022 Jul 15.

School of Materials Science and Engineering, the Key Laboratory of Material Processing and Mold of Ministry of Education, Henan Key Laboratory of Advanced Nylon Materials and Application, Zhengzhou University, Zhengzhou 450001, China.

The problem of nosocomial infections caused by bacterial growth on material surfaces is an urgent threat to public health. Although numerous materials and methods have been explored to fight against infections, the methods are complicated and the materials are slightly toxic. It is highly desirable to develop an antibacterial strategy that kills bacteria effectively without drug resistance and cytotoxicity. Herein, we present a synergistic antibacterial polylactic acid (PLA) surface with superhydrophobic antibacterial adhesion and photodynamic bactericidal activity. Initially, the surface displayed low-adhesion superhydrophobicity and resisted most bacterial adhesion. Furthermore, completely non-toxic chlorophyll possessed excellent photodynamic bactericidal properties under non-toxic visible light, which was incorporated into micro-/nanoscale PLA surfaces. We achieved efficient antibacterial activity using completely non-toxic materials and a facile non-solvent-induced phase separation process. This non-toxic, simple, good biocompatible, and no drug-resistant strategy has great advantages in combating bacterial infections.
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http://dx.doi.org/10.1021/acs.langmuir.2c01377DOI Listing
July 2022

Add drop multiplexers for terahertz communications using two-wire waveguide-based plasmonic circuits.

Nat Commun 2022 Jul 14;13(1):4090. Epub 2022 Jul 14.

Department of Engineering Physics, École Polytechnique de Montréal, Montreal, QC, H3T 1J4, Canada.

Terahertz (THz) band is considered to be the next frontier in wireless communications. The emerging THz multiplexing techniques are expected to dramatically increase the information capacity of THz communications far beyond a single channel limit. In this work, we explore the THz frequency-division multiplexing modality enabled by an add-drop multiplexer (ADM) design. Based on modular two-wire plasmonic waveguides fabricated using additive manufacturing and metallization techniques, we demonstrate four-port THz ADMs containing grating-loaded side couplers for operation at ~140 GHz carrier frequency. Particular attention is paid to the design of plasmonic waveguide Bragg gratings and directional couplers capable of splitting broadband THz light into spectral and spatial domains. Finally, we demonstrate multi/demultiplexing of THz signals with bit rates up to 6 Gbps using the developed ADMs. We believe that the proposed plasmonic circuits hold strong potential to provide robust integrated solutions for analog signal processing in the upcoming THz communications.
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http://dx.doi.org/10.1038/s41467-022-31590-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283530PMC
July 2022

Neuroimmune Regulation in Sepsis-Associated Encephalopathy: The Interaction Between the Brain and Peripheral Immunity.

Front Neurol 2022 27;13:892480. Epub 2022 Jun 27.

Translational Medicine Research Center, Medical Innovation Research Division and Fourth Medical Center of the Chinese PLA General Hospital, Beijing, China.

Sepsis-associated encephalopathy (SAE), the most popular cause of coma in the intensive care unit (ICU), is the diffuse cerebral damage caused by the septic challenge. SAE is closely related to high mortality and extended cognitive impairment in patients in septic shock. At present, many studies have demonstrated that SAE might be mainly associated with blood-brain barrier damage, abnormal neurotransmitter secretion, oxidative stress, and neuroimmune dysfunction. Nevertheless, the precise mechanism which initiates SAE and contributes to the long-term cognitive impairment remains largely unknown. Recently, a growing body of evidence has indicated that there is close crosstalk between SAE and peripheral immunity. The excessive migration of peripheral immune cells to the brain, the activation of glia, and resulting dysfunction of the central immune system are the main causes of septic nerve damage. This study reviews the update on the pathogenesis of septic encephalopathy, focusing on the over-activation of immune cells in the central nervous system (CNS) and the "neurocentral-endocrine-immune" networks in the development of SAE, aiming to further understand the potential mechanism of SAE and provide new targets for diagnosis and management of septic complications.
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http://dx.doi.org/10.3389/fneur.2022.892480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271799PMC
June 2022

Effects of probiotic administration on the digestibility characteristics and growth performance of finishing beef cattle fed a total mixed ration containing different levels of corn stover.

Anim Sci J 2022 Jan;93(1):e13751

College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Heilongjiang Provincial Key Laboratory of Efficient Utilization of Feed Resources and Nutrition Manipulation in Cold Region, Daqing, China.

In this study, we aimed to assess the effects of probiotic administration on the digestibility characteristics and growth performance of finishing beef cattle fed a total mixed ration (TMR) containing different levels of corn stover. One hundred and sixty Simmental × Continental crossbred bulls were randomly allocated to two animal houses (80 bulls each) and randomly assigned four TMR differing in the level of corn stover-high (HCT) and low (LCT)-with or without probiotics in each animal house. Feeding HCT supplemented with probiotics increased (P < 0.05) the apparent digestibility of crude protein (CP), ether extract (EE), neutral detergent fiber (NDF), and acid detergent fiber (ADF). Regardless of probiotic supplementation, the nitrogen intake and fecal nitrogen levels of animals fed HCT were lower than those fed LCT (P < 0.05). Additionally, feeding probiotics increased (P < 0.05) the efficiency of ruminal fermentation, final body weight, and average daily gain (ADG) of animals, with this effect being stronger in animals fed HCT. In conclusion, supplementing probiotics with HCT has a positive effect on the growth of finishing beef cattle, thereby providing economic benefits.
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http://dx.doi.org/10.1111/asj.13751DOI Listing
January 2022

Regional homogeneity of adolescents with high-functioning autism spectrum disorder and its association with symptom severity.

Brain Behav 2022 Jul 11:e2693. Epub 2022 Jul 11.

Department of Child and Adolescent Psychiatry, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background And Purpose: Previous studies have revealed abnormal regional homogeneity (ReHo) in individuals with autism spectrum disorder (ASD); however, there is little consistency across the findings within these studies, partly due to small sample size and great heterogeneity among participants between studies. Additionally, few studies have explored the association between ReHo aberrance and clinical symptoms in individuals with ASD.

Methods: Forty-eight adolescents with high-functioning ASD and 63 group-matched typically developing (TD) controls received functional magnetic resonance imaging at rest. Group-level analysis was performed to detect differences in ReHo between ASD and TD. Evaluation of symptom severity in individuals with ASD was based on the Autism Behavior Checklist (ABC). Voxel-wise correlation analysis was undergone to examine the correlations between the symptom severity and ReHo map in individuals with ASD within brain areas with ReHo abnormalities.

Results: Compared with the TD controls, individuals with ASD exhibited increased ReHo in the bilateral anterior cingulate cortex, left caudate, right posterior cerebellum (cerebellar tonsil), and bilateral brainstem and decreased ReHo in the left precentral gyrus, left inferior parietal lobule, bilateral postcentral gyrus, and right anterior cerebellum (culmen). The correlation analysis indicated that the ReHo value in the brainstem was negatively associated with the ABC total scores and the scores of Relating factor, respectively.

Conclusions: Our findings indicated that widespread ReHo abnormalities occurred in ASD, shedding light on the underlying neurobiology of pathogenesis and symptomatology of ASD.
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http://dx.doi.org/10.1002/brb3.2693DOI Listing
July 2022

Energy Efficiency Optimization for SWIPT-Enabled IoT Network with Energy Cooperation.

Sensors (Basel) 2022 Jul 4;22(13). Epub 2022 Jul 4.

School of Electrical and Electronic Engineering, Chongqing University of Technology, Chongqing 400054, China.

As an advanced technology, simultaneous wireless information and power transfer (SWIPT), combined with the internet of things (IoT) devices, can effectively extend the online cycle of the terminal. To cope with the fluctuation of energy harvesting by the hybrid access points (H-AP), the energy cooperation base station is introduced to realize the sharing of renewable energy. In this paper, we study the SWIPT-enabled IoT networks with cooperation. Our goal is to maximize the energy efficiency of the system, and at the same time, we need to meet the energy harvesting constraints, user quality of service (QoS) constraints and transmission power constraints. We jointly solve the power allocation, time switching and energy cooperation problems. Because this problem is a nonlinear programming problem, it is difficult to solve directly, so we use the alternating variable method, the iterative algorithm is used to solve the power allocation and time switching problem, and the matching algorithm is used to solve the energy cooperation problem. Simulation results show that the proposed algorithm has obvious advantages in energy efficiency performance compared with the comparison algorithm. At the same time, it is also proved that the introduction of energy cooperation technology can effectively reduce system energy consumption and improve system energy efficiency.
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http://dx.doi.org/10.3390/s22135035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269765PMC
July 2022

Spine-GFlow: A hybrid learning framework for robust multi-tissue segmentation in lumbar MRI without manual annotation.

Comput Med Imaging Graph 2022 Jul 16;99:102091. Epub 2022 Jun 16.

Department of Orthopaedics and Traumatology, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China. Electronic address:

Most learning-based magnetic resonance image (MRI) segmentation methods rely on the manual annotation to provide supervision, which is extremely tedious, especially when multiple anatomical structures are required. In this work, we aim to develop a hybrid framework named Spine-GFlow that combines the image features learned by a CNN model and anatomical priors for multi-tissue segmentation in a sagittal lumbar MRI. Our framework does not require any manual annotation and is robust against image feature variation caused by different image settings and/or underlying pathology. Our contributions include: 1) a rule-based method that automatically generates the weak annotation (initial seed area), 2) a novel proposal generation method that integrates the multi-scale image features and anatomical prior, 3) a comprehensive loss for CNN training that optimizes the pixel classification and feature distribution simultaneously. Our Spine-GFlow has been validated on 2 independent datasets: HKDDC (containing images obtained from 3 different machines) and IVDM3Seg. The segmentation results of vertebral bodies (VB), intervertebral discs (IVD), and spinal canal (SC) are evaluated quantitatively using intersection over union (IoU) and the Dice coefficient. Results show that our method, without requiring manual annotation, has achieved a segmentation performance comparable to a model trained with full supervision (mean Dice 0.914 vs 0.916).
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http://dx.doi.org/10.1016/j.compmedimag.2022.102091DOI Listing
July 2022
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