Publications by authors named "Yang Cao"

1,452 Publications

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Sparsely Wiring Connectivity in the Upper Beta Band Characterizes the Brains of Top Swimming Athletes.

Front Psychol 2021 16;12:661632. Epub 2021 Jul 16.

Human Phenome Institute, State Key Laboratory of Medical Neurobiology and Ministry of Education (MOE) Frontiers Center for Brain Science, School of Life Science and Research Institute of Intelligent Complex Systems, Fudan University, Shanghai, China.

Human brains are extremely energy costly in neural connections and activities. However, it is unknown what is the difference in the brain connectivity between top athletes with long-term professional trainings and age-matched controls. Here we ask whether long-term training can lower brain-wiring cost while have better performance. Since elite swimming requires athletes to move their arms and legs at different tempos in time with high coordination skills, we selected an eye-hand-foot complex reaction (CR) task to examine the relations between the task performance and the brain connections and activities, as well as to explore the energy cost-efficiency of top athletes. Twenty-one master-level professional swimmers and 23 age-matched non-professional swimmers as controls were recruited to perform the CR task with concurrent 8-channel EEG recordings. Reaction time and accuracy of the CR task were recorded. Topological network analysis of various frequency bands was performed using the phase lag index (PLI) technique to avoid volume conduction effects. The wiring number of connections and mean frequency were calculated to reflect the wiring and activity cost, respectively. Results showed that professional athletes demonstrated better eye-hand-foot coordination than controls when performing the CR task, indexing by faster reaction time and higher accuracy. Comparing to controls, athletes' brain demonstrated significantly less connections and weaker correlations in upper beta frequency band between the frontal and parietal regions, while demonstrated stronger connectivity in the low theta frequency band between sites of F3 and Cz/C4. Additionally, athletes showed highly stable and low eye-blinking rates across different reaction performance, while controls had high blinking frequency with high variance. Elite athletes' brain may be characterized with energy efficient sparsely wiring connections in support of superior motor performance and better cognitive performance in the eye-hand-foot complex reaction task.
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http://dx.doi.org/10.3389/fpsyg.2021.661632DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322235PMC
July 2021

Psychological and Behavioural Consistency Value Seeking of Tourists in Niche Tourism: Nostalgia, Authenticity Perception, and Satisfaction.

Psychol Res Behav Manag 2021 24;14:1111-1125. Epub 2021 Jul 24.

China Tourism Academy, Beijing, 100008, People's Republic of China.

Introduction: This study proposed a model based on Izard's theory of differential emotions to examine the effects of heritage nostalgia on perceived authenticity towards tourist satisfaction and the moderation of self-congruity in Chinese niche tourism.

Methods: We examined descriptive statistics to obtain an overview of the sample by using computer program SPSS 22. Then, for evaluation, partial least squares structural equation modelling (PLS-SEM) was employed as the appropriate statistical tool, and the statistical approach was implemented using the Smart PLS 3.0 computer program.

Results: The results of partial least squares structural equation modelling showed that heritage nostalgia positively affects existential and object-based authenticity. Existential and object-based authenticity positively influences tourist satisfaction. Self-congruity positively moderates the relationship between existential or object-based authenticity and heritage nostalgia.

Conclusion: The linkage mechanism of tourists' psychology and behavior in heritage tourism has been explored, the internal mechanisms such as heritage nostalgia, tourists' authenticity and tourists' satisfaction have been discovered, and the internal mechanism of tourists' consistency of psychology and behavior has also been explained.
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http://dx.doi.org/10.2147/PRBM.S322348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319553PMC
July 2021

Epidemiological characteristics of Varicella before and after the implementation of two-dose vaccination schedule in Chaoyang District, Beijing, 2007-2019.

J Infect 2021 Jul 29. Epub 2021 Jul 29.

Chaoyang District Center for Disease Control and Prevention, Beijing, China. Electronic address:

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http://dx.doi.org/10.1016/j.jinf.2021.07.035DOI Listing
July 2021

Anterograde regulation of mitochondrial genes and FGF21 signaling by hepatic LSD1.

JCI Insight 2021 Jul 27. Epub 2021 Jul 27.

Department of Medicine, Physiology and Biophysics, University of California, Irvine, Irvine, United States of America.

Mitochondrial biogenesis and function are controlled by anterograde regulatory pathways involving more than one thousand nuclear-encoded proteins. Transcriptional networks controlling the nuclear-encoded mitochondrial genes remain to be fully elucidated. Here we show that histone demethylase LSD1 knockout from adult mouse liver (LSD1-LKO) reduces the expression of one-third of all nuclear-encoded mitochondrial genes and decreases mitochondrial biogenesis and function. LSD1-modulated histone methylation epigenetically regulates nuclear-encoded mitochondrial genes. Furthermore, LSD1 regulates gene expression and protein methylation of nicotinamide mononucleotide adenylyltransferase 1 (NMNAT1), which controls the final step of NAD+ synthesis and limits NAD+ availability in nucleus. Lsd1 knockout reduces NAD+-dependent SIRT1 and SIRT7 deacetylase activity, leading to hyperacetylation and hypofunctioning of GABPβ and PGC-1α, the major transcriptional factor/cofactor for nuclear-encoded mitochondrial genes. Despite the reduced mitochondrial function in liver, LSD1-LKO mice are protected from diet-induced hepatic steatosis and glucose intolerance, partially due to induction of hepatokine FGF21. Thus, LSD1 orchestrates a core regulatory network involving epigenetic modifications and NAD+ synthesis to control mitochondrial function and hepatokine production.
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http://dx.doi.org/10.1172/jci.insight.147692DOI Listing
July 2021

An in vivo study of the effect of c-Jun on intervertebral disc degeneration in rats.

Bioengineered 2021 Dec;12(1):4320-4330

Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Intervertebral disc degeneration (IDD) has been well-recognized as one of the causes of vast lower back pain. The objective of the current study intends to elucidate the influence and regulatory molecular mechanisms of c-Jun on IDD. This study established an IDD model of Sprague-Dawley (SD) rats by needle puncture. An LV5-c-Jun lentiviral vector was constructed and injected into rats' intervertebral disc (IVD) tissue to increase the c-Jun expression following the establishment of modeling. The pathological changes of IVD tissue structure and collagen fibers were visualized following the processes of hematoxylin-eosin (HE) staining method and transmission electron microscopy. Real-time PCR, western blot, immunohistochemistry, and ELISA assays were performed to detect the expression levels of TGF-β, TIMP-3, COL2A1, and inflammatory cytokines. The collagen fibers were arranged in parallel and the surface was smooth after overexpression, whereas the collagen fibers in the control group were disorderly arranged with a rough surface. The findings indicated that c-Jun was responsible for upregulating expression levels of TGF-β, TIMP-3, and COL2A1 in the mRNA and proteins, but simultaneously downregulating expression levels of inflammatory factors IL-1β, IL-17, IL-6, and TNF-α. c-Jun overexpression produced a positive effect on IDD, inhibited inflammatory response , and might delay the degeneration of IVD. Thus, c-Jun may act as a novel potential agent in treating IDD.
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http://dx.doi.org/10.1080/21655979.2021.1946459DOI Listing
December 2021

Phase I study of Ibrutinib and the CXCR4 antagonist Ulocuplumab in CXCR4 mutated Waldenstrom Macroglobulinemia.

Blood 2021 07 21. Epub 2021 Jul 21.

Harvard Medical School, United States.

MYD88 and CXCR4 mutations are common in Waldenström Macroglobulinemia (WM). Mutated CXCR4 (CXCR4Mut) impacts BTK-inhibitor response. We conducted a Phase I trial of the CXCR4-antagonist ulocuplumab with ibrutinib in this first-ever study to target CXCR4Mut in WM. Ibrutinib was initiated at 420 mg/day with Cycle 1 and continued until intolerance or progression; ulocuplumab was given cycles 1-6, with a 3+3 dose-escalation design. Each cycle was 4 weeks. Thirteen symptomatic patients, nine treatment-naive were enrolled. Twelve were evaluable for response. At best response, their median serum IgM declined from 5,574 to 1,114 mg/dL; bone marrow disease decreased from 65% to 10%; and hemoglobin increased from 10.1 to 14.2 g/dL (p<0.001). The major and VGPR response rates were 100% and 33%, respectively, with VGPRs observed at lower ulocuplumab dose cohorts. Median times to minor and major responses were 0.9 and 1.2 months, respectively. With a median follow-up of 22.4 months, the estimated 2-year PFS was 90%. The most frequent recurring Grade ≥2 adverse events included reversible thrombocytopenia, rash, and skin infections. Ulocuplumab dose-escalation did not impact adverse events. The study demonstrates the feasibility of combining a CXCR4-antagonist with ibrutinib, and provides support for the development of CXCR4-antagonists for CXCR4Mut WM. www.clinicaltrials.gov (NCT03225716).
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http://dx.doi.org/10.1182/blood.2021012953DOI Listing
July 2021

Reviving the "Schottky" Barrier for Flexible Polymer Dielectrics with a Superior 2D Nanoassembly Coating.

Adv Mater 2021 Jul 19:e2101374. Epub 2021 Jul 19.

Polymer Program, Institute of Materials Science, University of Connecticut, Storrs, CT, 06269, USA.

The organic insulator-metal interface is the most important junction in flexible electronics. The strong band offset of organic insulators over the Fermi level of electrodes should theoretically impart a sufficient impediment for charge injection known as the Schottky barrier. However, defect formation through Anderson localization due to topological disorder in polymers leads to reduced barriers and hence cumbersome devices. A facile nanocoating comprising hundreds of highly oriented organic/inorganic alternating nanolayers is self-coassembled on the surface of polymer films to revive the Schottky barrier. Carrier injection over the enhanced barrier is further shunted by anisotropic 2D conduction. This new interface engineering strategy allows a significant elevation of the operating field for organic insulators by 45% and a 7× improvement in discharge efficiency for Kapton at 150 °C. This superior 2D nanocoating thus provides a defect-tolerant approach for effective reviving of the Schottky barrier, one century after its discovery, broadly applicable for flexible electronics.
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http://dx.doi.org/10.1002/adma.202101374DOI Listing
July 2021

Predictors of drug survival: A cohort study comparing anti-tumour necrosis factor agents using the Swedish inflammatory bowel disease quality register.

Aliment Pharmacol Ther 2021 Jul 19. Epub 2021 Jul 19.

Örebro, Sweden.

Background: Whether long-term effectiveness differs between anti-tumour necrosis factor (anti-TNF) agents is unknown.

Aims: To examine drug survival of first-line anti-TNF agents and identify predictors of discontinuation. To reduce channelling bias, we also compared drug survival of the second anti-TNF.

Methods: Biologic-naïve patients (N = 955) recorded in the Swedish IBD Quality Register (SWIBREG) were examined. We used propensity score matching, comparing drug survival over up to three years of follow-up. Cox regression estimated adjusted hazard ratios (aHRs) with 95% confidence intervals (CIs).

Results: In Crohn's disease, discontinuation because of lack/loss of response was 32% [95%CI = 26%-38%] for infliximab versus 16% [95%CI = 11%-21%] for adalimumab. Infliximab [vs adalimumab; aHR = 1.96; 95%CI = 1.20-3.21] and colonic disease (L2) [vs no L2; aHR = 2.17; 95% CI = 1.26-3.75] were associated with higher discontinuation rates, whereas normalised CRP at three months [aHR = 0.40; 95% CI = 0.19-0.81] with a lower rate. Consistently, patients who switched from adalimumab to infliximab (vs infliximab to adalimumab) had earlier discontinuation (P = 0.04). Concomitant use of immunomodulators was associated with a lower adverse drug reaction-mediated discontinuation rate [aHR = 0.46; 95% CI = 0.28-0.77], in part explained by fewer infusion reactions [aHR = 0.27; 95% CI = 0.08-0.89]. In ulcerative colitis, the probability of discontinuation because of lack/loss of response was 40% [95% CI = 33%-47%] for infliximab versus 37% [95% CI = 21%-53%] for adalimumab. Disease duration ≥10 years [aHR = 0.25; 95% CI = 0.10-0.58] and normalised CRP after three months [aHR = 0.39; 95% CI = 0.18-0.84] were associated with lower discontinuation rates.

Conclusions: Clinical characterisation of patients may aid decision-making on anti-TNF treatment. The consistently shorter drug survival for infliximab (vs adalimumab) in Crohn's disease, suggests a potential difference between the two drugs.
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http://dx.doi.org/10.1111/apt.16525DOI Listing
July 2021

Phosphine Sulfide-Based Bipolar Host Materials for Blue Phosphorescent Organic Light-Emitting Diodes.

Molecules 2021 Jul 3;26(13). Epub 2021 Jul 3.

State Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Jiangsu National Synergistic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.

Three phosphine sulfide-based bipolar host materials, , , and , were facilely prepared through a one-pot synthesis in excellent yields. The developed hosts exhibit superior thermal stabilities with the decomposition temperatures () all exceeding 350 °C and the melting temperatures () over 200 °C. In addition, their triplet energy () levels are estimated to be higher than 3.0 eV, illustrating that they are applicable to serve as hosts for blue phosphorescent organic light-emitting diodes (PhOLEDs). The maxima luminance, current efficiency (CE), power efficiency (PE), and external quantum efficiency (EQE) of 17,223 cd m, 36.7 cd A, 37.5 lm W, and 17.5% are achieved for the blue PhOLEDs hosted by . The PhOLEDs based on and show inferior device performance than that of , which might be ascribed to the deteriorated charge transporting balance as the increased number of the constructed carbazole units in and molecules would enhance the hole-transporting ability of the devices to a large extent. Our study demonstrates that the bipolar hosts derived from phosphine sulfide have enormous potential applications in blue PhOLEDs, and the quantity of donors should be well controlled to exploit highly efficient phosphine sulfide-based hosts.
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http://dx.doi.org/10.3390/molecules26134079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271492PMC
July 2021

Mitochondrial quality control in intervertebral disc degeneration.

Exp Mol Med 2021 Jul 16;53(7):1124-1133. Epub 2021 Jul 16.

Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Intervertebral disc degeneration (IDD) is a common and early-onset pathogenesis in the human lifespan that can increase the risk of low back pain. More clarification of the molecular mechanisms associated with the onset and progression of IDD is likely to help establish novel preventive and therapeutic strategies. Recently, mitochondria have been increasingly recognized as participants in regulating glycolytic metabolism, which has historically been regarded as the main metabolic pathway in intervertebral discs due to their avascular properties. Indeed, mitochondrial structural and functional disruption has been observed in degenerated nucleus pulposus (NP) cells and intervertebral discs. Multilevel and well-orchestrated strategies, namely, mitochondrial quality control (MQC), are involved in the maintenance of mitochondrial integrity, mitochondrial proteostasis, the mitochondrial antioxidant system, mitochondrial dynamics, mitophagy, and mitochondrial biogenesis. Here, we address the key evidence and current knowledge of the role of mitochondrial function in the IDD process and consider how MQC strategies contribute to the protective and detrimental properties of mitochondria in NP cell function. The relevant potential therapeutic treatments targeting MQC for IDD intervention are also summarized. Further clarification of the functional and synergistic mechanisms among MQC mechanisms may provide useful clues for use in developing novel IDD treatments.
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http://dx.doi.org/10.1038/s12276-021-00650-7DOI Listing
July 2021

Glycerol-3-phosphate biosynthesis regenerates cytosolic NAD to alleviate mitochondrial disease.

Cell Metab 2021 Jul 8. Epub 2021 Jul 8.

Graduate School of Peking Union Medical College, Beijing 100730, China; National Institute of Biological Sciences, Beijing 102206, China; Beijing Key Laboratory of Cell Biology for Animal Aging, Beijing 102206, China; Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing 102206, China. Electronic address:

Electron transport chain (ETC) dysfunction or hypoxia causes toxic NADH accumulation. How cells regenerate NAD under such conditions remains elusive. Here, integrating bioinformatic analysis and experimental validation, we identify glycerol-3-phosphate (Gro3P) biosynthesis as an endogenous NAD-regeneration pathway. Under genetic or pharmacological ETC inhibition, disrupting Gro3P synthesis inhibits yeast proliferation, shortens lifespan of C. elegans, impairs growth of cancer cells in culture and in xenografts, and causes metabolic derangements in mouse liver. Moreover, the Gro3P shuttle selectively regenerates cytosolic NAD under mitochondrial complex I inhibition; enhancing Gro3P synthesis promotes shuttle activity to restore proliferation of complex I-impaired cells. Mouse brain has much lower levels of Gro3P synthesis enzymes as compared with other organs. Strikingly, enhancing Gro3P synthesis suppresses neuroinflammation and extends lifespan in the Ndufs4 mice. Collectively, our results reveal Gro3P biosynthesis as an evolutionarily conserved coordinator of NADH/NAD redox homeostasis and present a therapeutic target for mitochondrial complex I diseases.
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http://dx.doi.org/10.1016/j.cmet.2021.06.013DOI Listing
July 2021

All-Organic Flexible Ferroelectret Nanogenerator with Fabric-Based Electrodes for Self-Powered Body Area Networks.

Small 2021 Jul 16:e2103161. Epub 2021 Jul 16.

Electrical Insulation Research Center, Institute of Materials Science, University of Connecticut, Storrs, CT, 06269, USA.

Due to their electrically polarized air-filled internal pores, optimized ferroelectrets exhibit a remarkable piezoelectric response, making them suitable for energy harvesting. Expanded polytetrafluoroethylene (ePTFE) ferroelectret films are laminated with two fluorinated-ethylene-propylene (FEP) copolymer films and internally polarized by corona discharge. Poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS)-coated spandex fabric is employed for the electrodes to assemble an all-organic ferroelectret nanogenerator (FENG). The outer electret-plus-electrode double layers form active device layers with deformable electric dipoles that strongly contribute to the overall piezoelectric response in the proposed concept of wearable nanogenerators. Thus, the FENG with spandex electrodes generates a short-circuit current which is twice as high as that with aluminum electrodes. The stacking sequence spandex/FEP/ePTFE/FEP/ePTFE/FEP/spandex with an average pore size of 3 µm in the ePTFE films yields the best overall performance, which is also demonstrated by the displacement-versus-electric-field loop results. The all-organic FENGs are stable up to 90 °C and still perform well 9 months after being polarized. An optimized FENG makes three light emitting diodes (LEDs) blink twice with the energy generated during a single footstep. The new all-organic FENG can thus continuously power wearable electronic devices and is easily integrated, for example, with clothing, other textiles, or shoe insoles.
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http://dx.doi.org/10.1002/smll.202103161DOI Listing
July 2021

Impacts of the COVID-19 epidemic on the department of stomatology in a tertiary hospital: A case study in the General Hospital of the Central Theater Command, Wuhan, China.

Community Dent Oral Epidemiol 2021 Jul 16. Epub 2021 Jul 16.

Clinical Epidemiology and Biostatistics, School of Medical Sciences, Örebro University, Örebro, Sweden.

Objective: The impact of the worldwide COVID-19 pandemic on the dental community is evident. Dental education programmes and academic activities have suffered from the ramifications of the pandemic. This study aimed to depict the impacts of the COVID-19 epidemic on the clinical services and academic activities in the department of stomatology of a tertiary hospital in Wuhan, China.

Methods: We obtained historical data of the Department of Stomatology from the Health Information System of the General Hospital of Central Theater Command, Wuhan, China between January 2018 and June 2020. Mean, standard deviation and median with interquartile range were used to summarize the variables. Line plots were used to illustrate the temporal trend. The Kruskal-Wallis equality-of-populations rank test was used to compare the difference between groups.

Results: A significant decrease was noted in the monthly average number of patients seeking outpatient services for the year 2020, which were decreased by two-thirds from 2018 to 2020. The number of emergency cases also decreased significantly by 57.6% in 2020. The monthly number of teaching hours decreased from 3.8 ± 1.5 in 2018 and 4.7 ± 1.4 in 2019 to 1.7 ± 1.9 in 2020. The number of interns also decreased by more than 77.0% in 2020.

Conclusions: The impacts of COVID-19 in the stomatology clinic were significant with notable decreases in clinical services and education offered to the stomatology students. There is a need to find solutions to keep as many dental professionals as needed remaining on the frontline of oral health care.
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http://dx.doi.org/10.1111/cdoe.12680DOI Listing
July 2021

Sequential CD19/22 CAR T-cell immunotherapy following autologous stem cell transplantation for central nervous system lymphoma.

Blood Cancer J 2021 Jul 15;11(7):131. Epub 2021 Jul 15.

Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Chimeric antigen receptor (CAR) T-cell immunotherapy following autologous stem cell transplantation (ASCT) is a promising method for refractory or relapsed multiple myeloma, but explicit data for central nervous system lymphoma (CNSL) are lacking. Here, we treated 13 CNSL patients with ASCT sequential CD19/22 CAR T-cell infusion and simultaneously evaluated the clinical efficacy and toxicity. The 13 CNSL patients analyzed included four primary CNSL and nine secondary CNSL patients. Patients 1 and 10, who had complete remission status before enrollment, maintained clinical efficacy without recurrence. Nine of the remaining 11 patients responded to our protocol with a median durable time of 14.03 months, and the overall response and complete remission rate were 81.81% and 54.55%, respectively. No patient suffered grades 3-4 cytokine-release syndrome (CRS), and only patient 10 experienced severe immune effector cell-associated neurotoxicity syndrome (ICANS). In addition, increases in serum ferritin and interleukin-6 levels were often accompanied by CRS and ICANS. After a median follow-up time of 14.20 months, the estimated 1-year progression-free survival and overall survival rates were 74.59% and 82.50%, respectively. Sequential CD19/22 CAR T-cell immunotherapy following ASCT as a novel method for CNSL appears to have encouraging long-term efficacy with relatively manageable side effects.
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http://dx.doi.org/10.1038/s41408-021-00523-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282870PMC
July 2021

Beta-Blocker Therapy Is Associated With Increased 1-Year Survival After Hip Fracture Surgery: A Retrospective Cohort Study.

Anesth Analg 2021 Jul 14. Epub 2021 Jul 14.

School of Medical Sciences, Orebro University, Orebro, Sweden.

Background: The high mortality rates seen within the first postoperative year after hip fracture surgery have remained relatively unchanged in many countries for the past 15 years. Recent investigations have shown an association between beta-blocker (BB) therapy and a reduction in risk-adjusted mortality within the first 90 days after hip fracture surgery. We hypothesized that preoperative, and continuous postoperative, BB therapy may also be associated with a decrease in mortality within the first year after hip fracture surgery.

Methods: In this retrospective cohort study, all adults who underwent primary emergency hip fracture surgery in Sweden, between January 1, 2008 and December 31, 2017, were included. Patients with pathological fractures and conservatively managed hip fractures were excluded. Patients who filled a prescription within the year before and after surgery were defined as having ongoing BB therapy. The primary outcome of interest was postoperative mortality within the first year. To reduce the effects of confounding from covariates due to nonrandomization in the current study, the inverse probability of treatment weighting (IPTW) method was used. Subsequently, Cox proportional hazards models were fitted to the weighted cohorts. These analyses were repeated while excluding patients who died within the first 30 days postoperatively. This reduces the effect of early deaths due to surgical and anesthesiologic complications as well as the higher degree of advanced directives present in the study population compared to the general population, which allowed for the evaluation of the long-term association between BB therapy and mortality in isolation. Results are reported as hazard ratios (HR) with 95% confidence intervals (CI). Statistical significance was defined as a 2-sided P value <.05.

Results: A total of 134,915 cases were included in the study. After IPTW, BB therapy was associated with a 42% reduction the risk of mortality within the first postoperative year (adjusted HR = 0.58, 95% CI, 0.57-0.60; P < .001). After excluding patients who died within the first 30 days postoperatively, BB therapy was associated with a 27% reduction in the risk of mortality (adjusted HR = 0.73, 95% CI, 0.71-0.75; P < .001).

Conclusions: A significant reduction in the risk of mortality in the first year following hip fracture surgery was observed in patients with ongoing BB therapy. Further investigations into this finding are warranted.
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http://dx.doi.org/10.1213/ANE.0000000000005659DOI Listing
July 2021

Ferrite-encapsulated nanoparticles with stable photothermal performance for multimodal imaging-guided atherosclerotic plaque neovascularization therapy.

Biomater Sci 2021 Jul 13. Epub 2021 Jul 13.

Department of Cardiology, The First Affiliated Hospital, Cardiovascular Institute, Harbin Medical University, Harbin 150001, P. R. China. and Department of Pathophysiology and Key Laboratory of Cardiovascular Pathophysiology, Harbin Medical University, Key Laboratory of Cardiovascular Medicine Research (Harbin Medical University), Ministry of Education, Harbin 150086, P. R. China.

Pathological angiogenesis is a critical contributor to atherosclerotic plaque rupture. However, there are few effective theranostic strategies to stabilize plaques by suppressing neovascularization. In this study, we fabricated a polymeric nanosystem using 3 nm manganese ferrite (MnFe2O4) and perfluorohexane (PFH) stabilized by polylactic acid-glycolic acid (PLGA) shells and conjugated to the surface of an anti-vascular endothelial growth factor receptor 2 (VEGFR2) antibody [ramucirumab (Ram)]. The [email protected]/MnFe2O4-Ram nanoparticles (NPs) were used as atherosclerotic plaque angiogenesis theranostics for multimodal imaging-guided photothermal therapy (PTT). Three-nanometer MnFe2O4 is an excellent magnetic resonance imaging T1 and photoacoustic imaging contrast agent. Upon exposure to near-infrared (NIR) light, MnFe2O4 in the NPs could transform NIR light into thermal energy for the photothermal elimination of plaque angiogenesis. Additionally, optical droplet vaporization of PFH in the NPs triggered by the thermal effect to form gas bubbles enhanced ultrasound imaging. Our in vitro experiments revealed that [email protected]/MnFe2O4-Ram NPs actively accumulated in rabbit aortic endothelial cells, and NP-mediated PTT promoted endothelial cell apoptosis while inhibiting their proliferation, migration, and tubulogenesis. Notably, the [email protected]/MnFe2O4-Ram NPs possessed excellent photostability and biocompatibility. In the rabbit advanced atherosclerotic plaque model, [email protected]/MnFe2O4-Ram NP-guided PTT significantly induced apoptosis of neovascular endothelial cells and improved the hypoxia status in the plaque 3 days after treatment. On day 28, PTT significantly reduced the density of neovessels and subsequently stabilized rabbit plaques by inhibiting plaque hemorrhage and macrophage infiltration. Collectively, these results suggest that [email protected]/MnFe2O4-Ram NP-guided PTT is a safe and effective theranostic strategy for inhibiting atherosclerotic plaque angiogenesis.
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http://dx.doi.org/10.1039/d1bm00343gDOI Listing
July 2021

Rejuvenation of Senescent Bone Marrow Mesenchymal Stromal Cells by Pulsed Triboelectric Stimulation.

Adv Sci (Weinh) 2021 Jul 14:e2100964. Epub 2021 Jul 14.

Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Stem cell senescence contributes to stem cell exhaustion and drives various aging-associated disorders. However, strategies to rejuvenate senescent stem cells are limited. The present study proposes an approach based on triboelectric stimulation to rejuvenate senescent bone marrow mesenchymal stromal cells (BMSCs) by fabricating a pulsed triboelectric nanogenerator (P-TENG) that can produce stable pulsed current output unaffected by the triggered frequency. The senescence phenotypes of aged BMSCs are reversed by triboelectric stimulation at 30 µA at 1.5 Hz. Triboelectric stimulation enhances the proliferation of aged BMSCs and increases their pluripotency and differentiation capacity. Additionally, mechanistic investigations reveal that pulsed triboelectric stimulation by P-TENG rejuvenates senescent BMSCs by enhancing MDM2-dependent p53 degradation, which is demonstrated by loss-of-function studies of MDM2 and p53. Overall, this study identifies a new approach for the rejuvenation of senescent BMSCs and describes a promising therapeutic intervention for many diseases associated with aged BMSCs.
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http://dx.doi.org/10.1002/advs.202100964DOI Listing
July 2021

The Interaction Between Per-admission β-Blocker Therapy, The Revised Cardiac Risk Index, and Mortality in Geriatric Hip Fracture Patients.

J Trauma Acute Care Surg 2021 Jul 9. Epub 2021 Jul 9.

Department of Orthopedic Surgery; Division of Trauma and Emergency Surgery, Department of Surgery, Orebro University Hospital; and School of Medical Sciences, Orebro University, Orebro, Sweden.

Background: An association between beta-blocker (BB) therapy and a reduced risk of major cardiac events and mortality in patients undergoing surgery for hip fractures has previously been demonstrated. Furthermore, a relationship between an increased Revised Cardiac Risk Index (RCRI) score and a higher risk of postoperative mortality has also been detected. The purpose of the current study was to investigate the interaction between BB therapy and RCRI in relation to 30-day postoperative mortality in geriatric patients after hip fracture surgery.

Methods: All patients over 65 years of age who underwent primary emergency hip fracture surgery in Sweden between January 1, 2008 and December 31, 2017, except for pathological fractures, were included in this retrospective cohort study. Patients were divided into cohorts based on their RCRI score (RCRI 1, 2, 3, and ≥ 4) and whether they had ongoing BB therapy at the time of admission. A Poisson regression model with robust standard errors of variance was used, while adjusting for confounders, to evaluate the association between BB therapy, RCRI, and 30-day mortality.

Results: A total of 126,934 cases met the study inclusion criteria. Beta-blocker therapy was associated with a 65% decrease in the risk of 30-day postoperative mortality in the whole study population [adj. IRR (95% CI): 0.35 (0.32-0.38), p < 0.001]. The use of BB also resulted in a significant reduction in 30-day postoperative mortality within all RCRI cohorts. However, the most pronounced effect of beta-blocker therapy was seen in patients with an RCRI score greater than 0.

Conclusions: Beta-blocker therapy is associated with a reduction in 30-day postoperative mortality, irrespective of RCRI score. Furthermore, patients with an elevated cardiac risk appear to have a greater benefit of beta-blocker therapy.

Level Of Evidence: Level II, Therapeutic / Care Management.
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http://dx.doi.org/10.1097/TA.0000000000003358DOI Listing
July 2021

Roadmap and Direction toward High-Performance MoS Hydrogen Evolution Catalysts.

Authors:
Yang Cao

ACS Nano 2021 Jul 12. Epub 2021 Jul 12.

Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing, 100871 P. R. China.

MoS intrinsically show Pt-like hydrogen evolution reaction (HER) performance. Pristine MoS displayed low HER activity, which was caused by low quantities of catalytic sites and unsatisfactory conductivity. Then, phase engineering and S vacancy were developed as effective strategies to elevate the intrinsic HER performance. Heterojunctions and dopants were successful strategies to improve HER performance significantly. A couple of state-of-the-art MoS catalysts showed HER performance comparable to Pt. Applying multiple strategies in the same electrocatalyst was the key to furnish Pt-like HER performance. In this review, we summarize the available strategies to fabricate superior MoS HER catalysts and tag the important works. We analyze the well-defined strategies for fabricating a superior MoS electrocatalyst, propose complementary strategies which could help meet practical requirements, and help people design highly efficient MoS electrocatalysts. We also provide a brief perspective on assembling practical electrochemical systems by high-performance MoS electrocatalysts, apply MoS in other important electrocatalysis reactions, and develop high-performance two-dimensional (2D) dichalcogenide HER catalysts not limited to MoS. This review will help researchers to obtain a better understanding of development of superior MoS HER electrocatalysts, providing directions for next-generation catalyst development.
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http://dx.doi.org/10.1021/acsnano.1c01879DOI Listing
July 2021

von Willebrand factor variants in C3 glomerulopathy: A Chinese cohort study.

Clin Immunol 2021 Aug 8;229:108794. Epub 2021 Jul 8.

Renal Division, Department of Medicine, Peking University First Hospital; Institute of Nephrology, Peking University, Key Laboratory of Renal Disease, Ministry of Health of China; Key Laboratory of CKD Prevention and Treatment, Ministry of Education of China; Beijing 100034, PR China; Peking-Tsinghua Center for Life Sciences, PR China.

C3 glomerulopathy (C3G) is a rare renal disease characterized by predominant glomerular C3 staining. Complement alternative pathway dysregulation due to inherited complement defects is associated with C3G. To identify novel C3G-related genes, we screened 86 genes in the complement, coagulation and endothelial systems in 35 C3G patients by targeted genomic enrichment and massively parallel sequencing. Surprisingly, the most frequently mutated gene was VWF. Patients with VWF variants had significantly higher proteinuria levels, higher crescent formation and lower factor H (FH) levels. We further selected two VWF variants to transiently express the von Willebrand factor (vWF) protein, we found that vWF expression from the c.1519A > G variant was significantly reduced. In vitro results further indicated that vWF could regulate complement activation, as it could bind to FH and C3b, act as a cofactor for factor I-mediated cleavage of C3b. Thus, we speculated that vWF might be involved in the pathogenesis of C3G.
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http://dx.doi.org/10.1016/j.clim.2021.108794DOI Listing
August 2021

TIDB: a comprehensive database of trained immunity.

Database (Oxford) 2021 Jul;2021

Department of Environmental Medicine, Tianjin Institute of Environmental and Operational Medicine, No.1 Dali Road, Heping District, Tianjin 300050, China.

Trained immunity is a newly emerging concept that defines the ability of the innate immune system to form immune memory and provide long-lasting protection against previously encountered antigens. Accumulating evidence reveals that trained immunity not only has broad benefits to host defense but is also harmful to the host in chronic inflammatory diseases. However, all trained immunity-related information is scattered in the literature and thus is difficult to access. Here, we describe Trained Immunity DataBase (TIDB), a comprehensive database that provides well-studied trained immunity-related genes from human, rat and mouse as well as the related literature evidence. Moreover, TIDB also provides three modules to analyze the function of the trained-immunity-related genes of interest, including Reactome pathway over-representation analysis, Gene Ontology enrichment analysis and protein-protein interaction subnetwork reconstruction. We believe TIDB will help developing valuable strategies for vaccine design and immune-mediated disease therapy. Database URL: http://www.ieom-tm.com/tidb.
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http://dx.doi.org/10.1093/database/baab041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271126PMC
July 2021

Novel high voltage polymer insulators using computational and data-driven techniques.

J Chem Phys 2021 May;154(17):174906

School of Materials Science and Engineering, Georgia Institute of Technology, 771 Ferst Drive NW, Atlanta, Georgia 30332, USA.

One of the key bottlenecks in the development of high voltage electrical systems is the identification of suitable insulating materials capable of supporting high voltages. Under high voltage scenarios, conventional polymer based insulators, which are one of the popular choices of insulators, suffer from the drawback of space charge accumulation, which leads to degradation in desirable electronic properties and facilitates dielectric breakdown. In this work, we aid the development of novel polymers for high voltage insulation applications by enabling the rapid prediction of properties that are correlated with dielectric breakdown, i.e.,the bandgap (E) of the polymer and electron injection barrier (Φ) at the electrode-insulator interface. To accomplish this, density functional theory based methods are used to develop large, chemically diverse datasets of Φ and E. The deviation of the computed properties from experimental observations is addressed using a statistical technique called Bayesian calibration. Furthermore, to enable rapid estimation of these properties for a large set of polymers, machine learning models are developed using the created dataset. These models are further used to predict E and Φ for a set of 13k previously known polymers. Polymers with high values of these properties are selected as potential high voltage insulators and are recommended for synthesis. Finally, the models developed here are deployed at www.polymergenome.org to enable the community use.
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http://dx.doi.org/10.1063/5.0044306DOI Listing
May 2021

Autophagy-Based Unconventional Secretory for AIM2 Inflammasome Drives DNA Damage Resistance During Intervertebral Disc Degeneration.

Front Cell Dev Biol 2021 22;9:672847. Epub 2021 Jun 22.

Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Intervertebral disc degeneration (IDD) is the primary cause of low back pain. Stress-induced DNA damage is closely relevant to the pathogenesis of IDD; however, the underlying mechanisms remain unclear. This study investigated the role of the absent in melanoma 2 (AIM2) inflammasome as a DNA damage sensor in nucleus pulposus (NP) cells. We found that the level of AIM2 increased in degenerated discs and was correlated to the degree of IDD. Knockdown of AIM2 ameliorated HO-induced DNA damage and apoptosis in NP cells , and retarded the progression of IDD . Furthermore, the induction of autophagy protected against cellular DNA damage via the unconventional secretion of AIM2. We further identified the Golgi re-assembly and stacking protein 55 (GRASP55) as mediator of the transport and secretion of AIM2 via an autophagic pathway. Taken together, our researches illustrate the role and regulatory mechanism of the AIM2 inflammasome during IDD. Targeting the AIM2 inflammasome may offer a promising therapeutic strategy for patients with IDD.
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http://dx.doi.org/10.3389/fcell.2021.672847DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258158PMC
June 2021

Autophagic Degradation of Gasdermin D Protects against Nucleus Pulposus Cell Pyroptosis and Retards Intervertebral Disc Degeneration In Vivo.

Oxid Med Cell Longev 2021 17;2021:5584447. Epub 2021 Jun 17.

Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Intervertebral disc degeneration (IDD) is the primary culprit of low back pain and renders heavy social burden worldwide. Pyroptosis is a newly discovered form of programmed cell death, which is also involved in nucleus pulposus (NP) cells during IDD progression. Moderate autophagy activity is critical for NP cell survival, but its relationship with pyroptosis remains unknown. This study is aimed at investigating the relationship between autophagy and pyroptotic cell death. The pyroptosis executor N-terminal domain of gasdermin D (GSDMD-N) and inflammation-related proteins were measured in lipopolysaccharide- (LPS-) treated human NP cells. Inhibition of autophagy by siRNA transfection and chemical drugs aggravated human NP cell pyroptosis. Importantly, we found that the autophagy-lysosome pathway and not the proteasome pathway mediated the degradation of GSDMD-N as lysosome dysfunction promoted the accumulation of cytoplasmic GSDMD-N. Besides, P62/SQSTM1 colocalized with GSDMD-N and mediated its degradation. The administration of the caspase-1 inhibitor VX-765 could reduce cell pyroptosis as confirmed in a rat disc IDD model , whereas ATG5 knockdown significantly accelerated the progression of IDD. In conclusion, our study indicated that autophagy protects against LPS-induced human NP cell pyroptosis via a P62/SQSTM1-mediated degradation mechanism and the inhibition of pyroptosis retards IDD progression . These findings deepen the understanding of IDD pathogenesis and hold implications in unraveling therapeutic targets for IDD treatment.
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http://dx.doi.org/10.1155/2021/5584447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238599PMC
June 2021

Prevalence, trends, and outcomes of atrial fibrillation in hospitalized patients with metastatic cancer: findings from a national sample.

Cancer Med 2021 Jul 7. Epub 2021 Jul 7.

Department of Health Statistics, Second Military Medical University, Shanghai, China.

Background: Epidemiological evidence regarding the link between cancer and atrial fibrillation (AF) are limited and outcomes of metastatic cancer comorbid with AF need to be elucidated.

Objective: This study aims to evaluate the prevalence, temporal trends, and outcomes of AF in hospitalized metastatic cancer patients.

Methods: The National Inpatient Sample (NIS) database was used to identify adult patients with metastatic tumors from 2003 to 2014. We analyzed the trends in AF prevalence, in-hospital mortality, total cost, length of stay (LOS), and comorbidities pertaining to metastatic cancer. Multivariable-adjusted models were used to evaluate the association of AF with clinical factors, in-hospital mortality, total cost, and LOS.

Results: Among 2,478,598 patients with metastatic cancer, 8.74% (216,737) were diagnosed with AF. The proportion of comorbid AF increased from 8.28% in 2003 to 10.06% in 2014 (p < 0.0001). Older age, white race, male, Medicare, higher income, larger hospital bed size, and urban teaching hospital were associated with higher AF occurrence. Among primary tumor sites, lung cancer experienced the highest odds of AF compared to other cancers. Patients with metastasis to lymph node and respiratory organ had higher odds of AF. In metastatic cancer, AF was associated with higher in-hospital mortality (odds ratio: 1.48; 95% confidence interval: 1.43-1.54), 18% longer LOS, and 19% higher cost.

Conclusions: AF prevalence in metastatic cancer continues to increase from 2003 to 2014. AF is linked to poorer prognosis and higher healthcare resource utilization. As the population ages, optimal preventive and treatment management strategies are needed for metastatic cancer comorbid with AF.
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http://dx.doi.org/10.1002/cam4.4105DOI Listing
July 2021

Effect of the Gene on Preadipocyte Differentiation in Sheep.

Front Genet 2021 21;12:649140. Epub 2021 Jun 21.

Institute of Animal Biotechnology, Jilin Academy of Agricultural Science, Changchun, China.

Acetyl-CoA acyltransferase 1 () functions as a key regulator of fatty acid β-oxidation in peroxisomes by catalyzing the cleavage of 3-ketoacyl-CoA to acetyl-CoA and acyl-CoA, which participate in the extension and degradation of fatty acids. Thus, is an important regulator of lipid metabolism and plays an essential role in fatty acid oxidation and lipid metabolism. Our previous study findings revealed that is closely associated with the peroxisome proliferator-activated receptor () signaling and fatty acid metabolism pathways, which are involved in fat deposition in sheep, leading to our hypothesis that may be involved in fat deposition by regulating lipid metabolism. However, the associated molecular mechanism remains unclear. In the present study, to assess the potential function of in sheep preadipocyte differentiation, we knocked down and overexpressed in sheep preadipocytes and evaluated the pattern of gene expression during preadipocyte differentiation by qRT-PCR. was significantly expressed in the early stage of adipocyte differentiation, and then its expression decreased. deficiency increased lipid accumulation and the triglyceride content and promoted sheep preadipocyte differentiation, whereas overexpression inhibited adipogenesis and decreased lipid accumulation and the triglyceride content. Simultaneously, we demonstrated that deficiency upregulated the expressions of the adipogenic marker genes γ and α in sheep preadipocytes, but overexpression inhibited the expressions of these markers, indicating that affects lipid metabolism by regulating adipogenic marker genes. Our results may promote a better understanding of the regulation of adipogenesis by .
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http://dx.doi.org/10.3389/fgene.2021.649140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255805PMC
June 2021

AXL kinase-mediated astrocytic phagocytosis modulates outcomes of traumatic brain injury.

J Neuroinflammation 2021 Jul 7;18(1):154. Epub 2021 Jul 7.

Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Jiefang Road88th, Hangzhou, 310016, China.

Background: Complex changes in the brain microenvironment following traumatic brain injury (TBI) can cause neurological impairments for which there are few efficacious therapeutic interventions. The reactivity of astrocytes is one of the keys to microenvironmental changes, such as neuroinflammation, but its role and the molecular mechanisms that underpin it remain unclear.

Methods: Male C57BL/6J mice were subjected to the controlled cortical impact (CCI) to develop a TBI model. The specific ligand of AXL receptor tyrosine kinase (AXL), recombinant mouse growth arrest-specific 6 (rmGas6) was intracerebroventricularly administered, and selective AXL antagonist R428 was intraperitoneally applied at 30 min post-modeling separately. Post-TBI assessments included neurobehavioral assessments, transmission electron microscopy, immunohistochemistry, and western blotting. Real-time polymerase chain reaction (RT-PCR), siRNA transfection, and flow cytometry were performed for mechanism assessments in primary cultured astrocytes.

Results: AXL is upregulated mainly in astrocytes after TBI and promotes astrocytes switching to a phenotype that exhibits the capability of ingesting degenerated neurons or debris. As a result, this astrocytic transformation promotes the limitation of neuroinflammation and recovery of neurological dysfunction. Pharmacological inhibition of AXL in astrocytes significantly decreased astrocytic phagocytosis both in vivo and in primary astrocyte cultures, in contrast to the effect of treatment with the rmGas6. AXL activates the signal transducer and activator of the transcription 1 (STAT1) pathway thereby further upregulating ATP-binding cassette transporter 1 (ABCA1). Moreover, the supernatant from GAS6-depleted BV2 cells induced limited enhancement of astrocytic phagocytosis in vitro.

Conclusion: Our work establishes the role of AXL in the transformation of astrocytes to a phagocytic phenotype via the AXL/STAT1/ABCA1 pathway which contributes to the separation of healthy brain tissue from injury-induced cell debris, further ameliorating neuroinflammation and neurological impairments after TBI. Collectively, our findings provide a potential therapeutic target for TBI.
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http://dx.doi.org/10.1186/s12974-021-02201-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264993PMC
July 2021

Donor-Acceptor Compensated ZnO Semiconductor for Photoelectrochemical Biosensors.

ACS Appl Mater Interfaces 2021 Jul 7;13(28):33006-33014. Epub 2021 Jul 7.

Key Laboratory of Advanced Materials of Tropical Island Resources, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China.

Hindering the recombination of a photogenerated carrier is a crucial method to enhance the photoelectrochemical performance of ZnO due to its high exciton binding energy. Herein, the intramolecular donor-acceptor compensated semiconductor ZnO (I-D/A ZnO), introducing C dopants and oxygen vacancies, was prepared with the assistance of ascorbic acid (AA). According to the DFT calculations, the asymmetry DOS could lead to the longer carrier lifetime and the smaller electron transfer resistance. Then, the photoelectrochemical biosensor toward glucose was regarded as a model to discuss the application of ZnO in biosensors. As a result, the biosensor based on I-D/A ZnO showed good performance with high sensitivity, low limit of detection, and fine anti-interference, meaning that I-D/A ZnO is a promising semiconductor for photoelectrochemical biosensors.
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http://dx.doi.org/10.1021/acsami.1c07499DOI Listing
July 2021

Amplified antitumor efficacy by a targeted drug retention and chemosensitization strategy-based "combo" nanoagent together with PD-L1 blockade in reversing multidrug resistance.

J Nanobiotechnology 2021 Jul 5;19(1):200. Epub 2021 Jul 5.

Department of Ultrasound, Chongqing Key Laboratory of Ultrasound Molecular Imaging, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, People's Republic of China.

Background: Recent studies have demonstrated that multidrug resistance (MDR) is a critical factor in the low efficacy of cancer chemotherapy. The main mechanism of MDR arises from the overexpression of P-glycoprotein (P-gp), which actively enhances drug efflux and limits the effectiveness of chemotherapeutic agents.

Results: In this study, we fabricated a "combo" nanoagent equipping with triple synergistic strategies for enhancing antitumor efficacy against MDR cells. Tumor homing-penetrating peptide endows the nanosystem with targeting and penetrating capabilities in the first stage of tumor internalization. The abundant amine groups of polyethylenimine (PEI)-modified nanoparticles then trigger a proton sponge effect to promote endo/lysosomal escape, which enhances the intracellular accumulation and retention of anticancer drugs. Furthermore, copper tetrakis(4-carboxyphenyl)porphyrin (CuTCPP) encapsulated in the nanosystem, effectively scavenges endogenous glutathione (GSH) to reduce the detoxification mediated by GSH and sensitize the cancer cells to drugs, while simultaneously serving as a photoacoustic imaging (PAI) contrast agent for image visualization. Moreover, we also verify that these versatile nanoparticles in combination with PD-1/PD-L1 blockade therapy can not only activate immunological responses but also inhibit P-gp expression to obliterate primary and metastatic tumors.

Conclusion: This work shows a significant enhancement in therapeutic efficacy against MDR cells and syngeneic tumors by using multiple MDR reversing strategies compared to an equivalent dose of free paclitaxel.
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http://dx.doi.org/10.1186/s12951-021-00947-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256488PMC
July 2021

Diagnostic Value of the Fimbriae Distribution Pattern in Localization of Urinary Tract Infection.

Front Med (Lausanne) 2021 18;8:602691. Epub 2021 Jun 18.

Key Laboratory of Immune Microenvironment and Disease of the Educational Ministry of China, Tianjin Key Laboratory of Cellular and Molecular Immunology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.

Urinary tract infections (UTIs) are one of the most common infectious diseases. UTIs are mainly caused by uropathogenic (UPEC), and are either upper or lower according to the infection site. Fimbriae are necessary for UPEC to adhere to the host uroepithelium, and are abundant and diverse in UPEC strains. Although great progress has been made in determining the roles of different types of fimbriae in UPEC colonization, the contributions of multiple fimbriae to site-specific attachment also need to be considered. Therefore, the distribution patterns of 22 fimbrial genes in 90 UPEC strains from patients diagnosed with upper or lower UTIs were analyzed using PCR. The distribution patterns correlated with the infection sites, an XGBoost model with a mean accuracy of 83.33% and a mean area under the curve (AUC) of the receiver operating characteristic (ROC) of 0.92 demonstrated that fimbrial gene distribution patterns could predict the localization of upper and lower UTIs.
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http://dx.doi.org/10.3389/fmed.2021.602691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249706PMC
June 2021
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