Publications by authors named "Yanchun Li"

159 Publications

Downregulation of CISD2 Has Prognostic Value in Non-Small Cell Lung Cancer and Inhibits the Tumorigenesis by Inducing Mitochondrial Dysfunction.

Front Oncol 2020 1;10:595524. Epub 2021 Feb 1.

Department of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.

CISD2, a NEET protein that coordinates 2Fe-2S clusters through its CDGSH domain, is critical for normal development and iron homeostasis. CISD2 plays an important role in Fe-S cluster transfer and promotes cancer proliferation. However, its specific role in the development of non-small cell lung cancer (NSCLC) remains unclear. Bioinformatics of pan-cancer analysis from The Cancer Genome Atlas show that CISD2 has an aberrant expression in most types of human cancers. Moreover, CISD2 expression is associated with a higher hazard ratio and exhibits significantly poorer overall survival in lung adenocarcinoma (LUAD), uveal melanoma, head and neck squamous cell carcinoma, brain lower grade glioma, kidney chromophobe, and liver hepatocellular carcinoma. Further investigation revealed that CISD2 is highly expressed in LUAD and LUSC, which is associated with clinical pathological stages. In addition, survival data collected from GSE31210 and GSE13213, two datasets from the NCBI Gene Expression Omnibus, also confirmed that high CISD2 expression is associated with unfavorable survival in patients with LUAD. A cell-based assay indicated that the knockdown of CISD2 inhibited proliferation, invasion, and migration in A549 cells. Additionally, CISD2 knockdown accelerated the accumulation of cellular and mitochondrial reactive oxygen species, destroying the mitochondrial morphology and function. Moreover, CISD2 inhibition activated the iron starvation response, thus, accelerating iron accumulation in A549 cells. Pretreatment with DFO, the iron chelator, blocked mitochondrial dysfunction in CISD2-knockdown cells. Collectively, the present study provides novel insights into the regulatory role of CISD2 in NSCLC and presents a potential target to improve antitumor activity based on oxidative stress.
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http://dx.doi.org/10.3389/fonc.2020.595524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882736PMC
February 2021

High-mechanical strength carboxymethyl chitosan-based hydrogel film for antibacterial wound dressing.

Carbohydr Polym 2021 Mar 4;256:117590. Epub 2021 Jan 4.

Institute for Biomass and Function Materials & National Demonstration Centre for Experimental Light Chemistry Engineering Education, College of Bioresources Chemistry and Materials Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China. Electronic address:

Hydrogels, being highly biocompatible and adaptable with biological tissues, have shown great usability in biomedical applications. In this research, a novel hydrogel film developed from carboxymethyl chitosan (CMCS) loaded with waterborne polyurethane-gelatin hydrolysate was synthesized via aqueous emulsion copolymerization. The synthesized hydrogel film was characterized using mechanical strength tests, FTIR, XPS, SEM, AFM, and various other analysis technologies. The results demonstrated that the hydrogel film exhibited good thermal stability, swelling behavior, as well as controllable biodegradability. Specifically, when the CMCS content was loaded at 6 %, the maximum tensile strength and elongation at the break of the hydrogel film were reached 31.69 MPa and 447.187, respectively. The disk diffusion tests indicated that the hydrogel film presented significant antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). These results indicate that hydrogel films with high mechanical strength and high antibacterial activity could be used for wound dressing applications.
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http://dx.doi.org/10.1016/j.carbpol.2020.117590DOI Listing
March 2021

A promising anticancer drug: a photosensitizer based on the porphyrin skeleton.

RSC Med Chem 2020 Apr 25;11(4):427-437. Epub 2020 Feb 25.

Institute of Pharmacy & Pharmacology , Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study , University of South China , Hengyang City , Hunan Province 421001 , P.R. China . Email:

Photodynamic therapy (PDT) is a minimally invasive combination of treatments that treat tumors and other diseases by using photosensitizers, light and oxygen to produce cytotoxic reactive oxygen species (ROS) inducing tumor cell apoptosis. Photosensitizers are the key part of PDT for clinical application and experimental research, and most of them are porphyrin compounds at present. Due to their unique affinity for tumor tissues, porphyrins are not only excellent photosensitizers, but also good carriers to transport other active drugs into tumor tissues, which can exert synergistic anticancer effects of PDT and chemotherapy. This article reviews the clinical development of porphyrin photosensitizers and the research status of porphyrin containing bioactive groups. Finally, future perspectives and the current challenges of photosensitizers based on the porphyrin skeleton are discussed.
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http://dx.doi.org/10.1039/c9md00558gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460723PMC
April 2020

Corrigendum to "Delivery of oncolytic adenovirus into the nucleus of tumorigenic cells by tumor microparticles for virotherapy" [Biomaterials 89C (2016) 56-66].

Biomaterials 2021 Feb 30;269:120619. Epub 2020 Dec 30.

National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing, 100005, China; Department of Biochemistry & Molecular Biology, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, 430030, China. Electronic address:

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http://dx.doi.org/10.1016/j.biomaterials.2020.120619DOI Listing
February 2021

Vitamin D/VDR attenuate cisplatin-induced AKI by down-regulating NLRP3/Caspase-1/GSDMD pyroptosis pathway.

J Steroid Biochem Mol Biol 2021 Feb 28;206:105789. Epub 2020 Nov 28.

Department of Nephrology, Third Xiangya Hospital, Central South University, Changsha, Hunan, 410013, China. Electronic address:

Vitamin D/Vitamin D receptor (VDR) has been shown to inhibit the NF-κB-mediated inflammatory effects. Up-regulation of the NLRP3(Recombinant NLR Family, Pyrin Domain Containing Protein 3)/Caspase-1/GSDMD (Gasdermin D) pathway through NF-κb is one of the key mechanisms leading to pyroptosis. This study aims to explore the effects of vitamin D/VDR on the pyroptosis pathway in cisplatin induced acute kidney injury (AKI) models. Our results showed that in wide type mice, renal function loss, tissue injury and cell death induced by cisplatin were alleviated by pretreatment of high-dose paricalcitol(a VDR agonist) accompanied with up-regulated VDR and decreased expression of NLRP3, GSDMD-N, Cleaved-Caspase-1 and mature Interleukin- 1β (features of pyroptosis). While, in VDR knock out mice, cisplatin induced more severer renal injury and further increased pyroptosis related protein than the wild type mice and the effect of paricalcitol were also eliminated. In tubular cell specific VDR-over expressing mice, those renal injury index as well as pyroptosis phenotype were significantly reduced by low-dose paricalcitol pretreatment with upregulated VDR expression compared with WT mice. In vitro data using gain and lose function experiments in Human tubular epithelial cell (HK-2) were consistent with the observation as in vivo work. Our further experiments in both animal and cell culture work has found that the level of IκBα(Inhibitor of NF-κB) were decreased and the nuclear level of NF-κB p65 of renal tubular cells were increased after cisplatin injury while VDR activation by paricalcitol could reverse up-regulation of nuclear NF-κB p65 with reduced cell pyroptosis. These data suggested that vitamin D/VDR could alleviate cisplatin-induced acute renal injury partly by inhibiting NF-κB-mediated NLRP3/Caspase-1/GSDMD pyroptosis.
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http://dx.doi.org/10.1016/j.jsbmb.2020.105789DOI Listing
February 2021

Prediction of topological nontrivial semimetals and pressure-induced Lifshitz transition in 1T'-MoS layered bulk polytypes.

Nanoscale 2020 Nov;12(44):22710-22717

Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.

Recently, bulk MoS2 crystals stacked by 1T'-MoS2 monolayers have been synthesized successfully, but little is known about their stacking sequences and topological properties. Based on first-principles calculations and symmetry-based indicator theory, we discovered that three predicted bulk structures of MoS2 (named 2M-, 1T'- and β-MoS2) stacked by 1T' monolayers are topological insulators and nodal line semimetals with and without spin-orbit coupling. Their stacking stability, electronic structure and the topology origin were systematically investigated. Further research proves that in the absence of SOC the open- and closed-type nodal lines can coexist in the momentum space of 2M-MoS2, which also possesses drumhead-like surface state. Moreover, we predicted a pressure-induced Lifshitz transition at about 1.3 GPa in 2M-MoS2. Our findings greatly enrich the topological phases of MoS2 and probably bring MoS2 to the rapidly growing family of layered topological semimetals.
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http://dx.doi.org/10.1039/d0nr05208fDOI Listing
November 2020

Sorafenib induces mitochondrial dysfunction and exhibits synergistic effect with cysteine depletion by promoting HCC cells ferroptosis.

Biochem Biophys Res Commun 2021 Jan 6;534:877-884. Epub 2020 Nov 6.

Department of Laboratory Medicine, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, China. Electronic address:

Hepatocellular carcinoma (HCC) is one of the most common malignant cancers worldwide. The prognosis of HCC remains poor. Currently, sorafenib is the first-line drug for advanced HCC. Although sorafenib's mechanism of action involving several established cancer-related protein kinase targets is well-characterized, the underlying molecular mechanism is still unclear. Here, we found that sorafenib inhibited viability, proliferation, and migration of HCC cells in a dose-dependent manner. Sorafenib treatment of HCC cells destroyed mitochondrial morphology, accompanied by decreased activity of oxidative phosphorylation, collapse of mitochondrial membrane potential, and reduced synthesis of ATP, with consequent cell death due to ferroptosis. Pharmacological utilization of glutathione (GSH) rescued the sorafenib-induced ferroptosis, eliminated the accumulation of cellular mitochondrial reactive oxygen species (ROS), and lipid peroxide. GSH depletion through cysteine deprivation or cysteinase inhibition exacerbated sorafenib-induced ferroptotic cell death and lipid peroxides generation, and enhanced oxidative stress and mitochondrial ROS accumulation. Collectively, these findings indicate that depletion of cysteine acts synergistically with sorafenib and renders HCC cells vulnerable to ferroptosis, presenting the potential value of new therapeutic combinations for advanced HCC.
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http://dx.doi.org/10.1016/j.bbrc.2020.10.083DOI Listing
January 2021

Anomalous mechanical materials squeezing three-dimensional volume compressibility into one dimension.

Nat Commun 2020 Nov 5;11(1):5593. Epub 2020 Nov 5.

Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.

Anomalous mechanical materials, with counterintuitive stress-strain responding behaviors, have emerged as novel type of functional materials with highly enhanced performances. Here we demonstrate that the materials with coexisting negative, zero and positive linear compressibilities can squeeze three-dimensional volume compressibility into one dimension, and provide a general and effective way to precisely stabilize the transmission processes under high pressure. We propose a "corrugated-graphite-like" structural model and discover lithium metaborate (LiBO) to be the first material with such a mechanical behavior. The capability to keep the flux density stability under pressure in LiBO is at least two orders higher than that in conventional materials. Our study opens a way to the design and search of ultrastable transmission materials under extreme conditions.
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http://dx.doi.org/10.1038/s41467-020-19219-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644688PMC
November 2020

Distance makes a difference in crystalline photoluminescence.

Nat Commun 2020 11 4;11(1):5572. Epub 2020 Nov 4.

Key Laboratory of Materials Physics, Anhui Key Laboratory of Nanomaterials and Nanotechnology, CAS Center for Excellence in Nanoscience, Institute of Solid State Physics, Chinese Academy of Sciences, 230031, Hefei, China.

Crystallization-induced photoluminescence weakening was recently revealed in ultrasmall metal nanoparticles. However, the fundamentals of the phenomenon are not understood yet. By obtaining conformational isomer crystals of gold nanoclusters, we investigate crystallization-induced photoluminescence weakening and reveal that the shortening of interparticle distance decreases photoluminescence, which is further supported by high-pressure photoluminescence experiments. To interpret this, we propose a distance-dependent non-radiative transfer model of excitation electrons and support it with additional theoretical and experimental results. This model can also explain both aggregation-induced quenching and aggregation-induced emission phenomena. This work improves our understanding of aggregated-state photoluminescence, contributes to the concept of conformational isomerism in nanoclusters, and demonstrates the utility of high pressure studies in nanochemistry.
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http://dx.doi.org/10.1038/s41467-020-19377-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643180PMC
November 2020

GPR40 deficiency is associated with hepatic FAT/CD36 upregulation, steatosis, inflammation, and cell injury in C57BL/6 mice.

Am J Physiol Endocrinol Metab 2021 01 26;320(1):E30-E42. Epub 2020 Oct 26.

Ralph H. Johnson Veterans Affairs Medical Center, Charleston, South Carolina.

G-protein-coupled receptor 40 (GPR40) is highly expressed in pancreatic islets, and its activation increases glucose-stimulated insulin secretion from pancreas. Therefore, GPR40 is considered as a target for type 2 diabetes mellitus (T2DM). Since nonalcoholic fatty liver disease (NAFLD) is associated with T2DM and GPR40 is also expressed by hepatocytes and macrophages, it is important to understand the role of GPR40 in NAFLD. However, the role of GPR40 in NAFLD in animal models has not been well defined. In this study, we fed wild-type or GPR40 knockout C57BL/6 mice a high-fat diet (HFD) for 20 wk and then assessed the effect of GPR40 deficiency on HFD-induced NAFLD. Assays on metabolic parameters showed that an HFD increased body weight, glucose, insulin, insulin resistance, cholesterol, and alanine aminotransferase (ALT), and GPR40 deficiency did not mitigate the HFD-induced metabolic abnormalities. In contrast, we found that GPR40 deficiency was associated with increased body weight, insulin, insulin resistance, cholesterol, and ALT in control mice fed a low-fat diet (LFD). Surprisingly, histology and Oil Red O staining showed that GPR40 deficiency in LFD-fed mice was associated with steatosis. Immunohistochemical analysis showed that GPR40 deficiency also increased F4/80, a macrophage biomarker, in LFD-fed mice. Furthermore, results showed that GPR40 deficiency led to a robust upregulation of hepatic fatty acid translocase (FAT)/CD36 expression. Finally, our in vitro studies showed that GPR40 knockdown by siRNA or a GPR40 antagonist increased palmitic acid-induced FAT/CD36 mRNA in hepatocytes. Taken together, this study indicates that GPR40 plays an important role in homeostasis of hepatic metabolism and inflammation and inhibits nonalcoholic steatohepatitis by possible modulation of FAT/CD36 expression.
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http://dx.doi.org/10.1152/ajpendo.00257.2020DOI Listing
January 2021

Phenanthroline Derivative Fluorescent Probe for Rapid and Sensitive Detection of Silver (I).

Anal Sci 2020 Oct 23. Epub 2020 Oct 23.

College of Chemistry, Jilin University.

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http://dx.doi.org/10.2116/analsci.20P338DOI Listing
October 2020

Hyaluronic acid and graphene oxide loaded silicon contact lens for corneal epithelial healing.

J Biomater Sci Polym Ed 2021 Feb 28;32(3):372-384. Epub 2020 Oct 28.

Department of Fundus Disease, The Second People's Hospital of Jinan, Jinan, Shandong Province, China.

Hyaluronic acid (HA) eye drop solution is widely used to treat and manage various corneal diseases like keratoconus (after corneal cross-linking) and dry eye syndrome. However, ocular dosage forms like eye drop solution affect the routine life style of patients due to frequent dosing schedule. In this study, HA and reduced graphene oxide (rGO) was directly loaded in the silicon contact lenses (HA-GO-DL) and compared with the conventional soaking method (HA-GO-SM). The contact lenses at lower level of rGO showed permissible swelling and transmittance properties. The water retention property of HA-GO-DL contact lenses was confirmed by water evaporation studies. The flux data of HA-GO-SM contact lenses showed high burst release with 24 h release duration. While, HA-GO-DL lenses confirmed low burst with sustained release up to 96 h. In ocular irritation study, the HA-GO-DL-2 lenses was found to be safe. The HA-GO-DL-2 batch showed high HA-tear fluid concentration (rabbit model) and improvement in the rabbit tear fluid volume (Schirmer strip studies) in comparison to the soaking method (HA-GO-SM-2) and eye drop solution. The study successfully demonstrate the potential of HA-GO loaded contact lenses to improve tear fluid volume to manage various ocular diseases like dry eye syndrome.
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http://dx.doi.org/10.1080/09205063.2020.1836926DOI Listing
February 2021

Alkaloid-based regimen is beneficial for acute myeloid leukemia resembling acute promyelocytic leukemia with NUP98/RARG fusion and RUNX1 mutation: A case report.

Medicine (Baltimore) 2020 Oct;99(40):e22488

Department of Hematology, Cancer Center, the First Hospital of Jilin University, Changchun.

Rationale: Some acute myeloid leukemia (AML) patients present with features mimicking the classical hypergranular subtype of acute promyelocytic leukemia (APL) but without the typical promyelocytic leukemia/retinoic acid receptor α (PML/RARα) rearrangement. Herein, we report an AML patient resembling APL but with nucleoporin 98/retinoid acid receptor gamma gene (NUP98/RARG) fusion transcript and Runt-related transcription factor 1 (RUNX1) mutation.

Patient Concerns: An 18-year-old male presented at the hospital with a diagnosis of AML.

Diagnoses: The patient was diagnosed with bone marrow examination. Bone marrow smear displayed 90.5% promyelocytes. Fluorescence in situ hybridization analysis failed to detect the PML/RARα fusion transcript or RARα amplification. While real-time polymerase chain reaction showed positivity for the NUP98/RARG fusion transcript. G-banding karyotype analysis showed a normal karyotype.

Interventions: The patient showed resistance to arsenic trioxide and standard 3 + 7 chemotherapy, but eventually achieved complete remission through the Homoharringtonine, Cytarabine, and Aclarubicin chemotherapy.

Outcomes: These measures resulted in a rapid response and disease control.

Lessons: Acute myeloid leukemia with the NUP98/RARG fusion gene and the RUNX1 mutation may be a special subtype of AML and may benefit from the alkaloid-based regimen.
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http://dx.doi.org/10.1097/MD.0000000000022488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7535657PMC
October 2020

TEOA Inhibits Proliferation and Induces DNA Damage of Diffuse Large B-Cell Lymphoma Cells Through Activation of the ROS-Dependent p38 MAPK Signaling Pathway.

Front Pharmacol 2020 4;11:554736. Epub 2020 Sep 4.

Phase I Clinical Research Center, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.

Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of lymphoma, accounting for approximately 30% to 40% of non-Hodgkin's lymphomas (NHL). The administration of rituximab significantly improved the outcomes of DLBCL; however, the unavoidable development of resistance limits the long-term efficacy. Therefore, a new generation of less toxic drugs with higher chemotherapy response is required to prevent or reverse chemoresistance. TEOA is a pentacyclic triterpenoid compound isolated from the roots of . Studies have confirmed that TEOA has significant cytotoxicity on gastrointestinal cancer cells. However, there are no relevant reports on DLBCL cells. In this study, we investigated the potential molecular mechanism of the anticancer activity of TEOA in DLBCL cells. The results demonstrated that TEOA inhibited proliferation and induced apoptosis in time-and dose-dependent manners. TEOA induced reactive oxygen species (ROS) generation, which was reversed by N-acetyl cysteine (NAC). TEOA induced DNA damage, increased the level of γ-H2AX, and the phosphorylation of CHK1 and CHK2. In addition, TEOA induced the activation of the p38 MAPK pathway and pretreated with p38 inhibitor SB20358 or ROS scavenger could block TEOA-induced DNA damage. Taken together, these results suggest that ROS mediated activation of the p38 MAPK signal pathway plays an important role in initiating TEOA-induced DNA damage.
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http://dx.doi.org/10.3389/fphar.2020.554736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500465PMC
September 2020

Expression of angiopoietin-like protein 2 in ovarian tissue of rat polycystic ovarian syndrome model and its correlation study.

Reprod Biol Endocrinol 2020 Sep 25;18(1):94. Epub 2020 Sep 25.

Reproduction Center, The Third Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, Henan, China.

Background: This study investigated the expression of angiopoietin-like protein 2 (ANGPTL2) in the tissues of rat models of polycystic ovary syndrome (PCOS) and its correlation with PCOS.

Methods: Six-weeks-old female specific pathogen-free rats (n = 60) were divided into blank control, PCOS model, and metformin groups (n = 20/group). After 21 days of metformin intervention, the serum sex hormones, fasting blood glucose, fasting insulin, and insulin resistance (IR) of rats in each group were measured. The mRNA levels of ANGPTL2, Foxol, and Akt in the ovarian tissues were monitored by real-time fluorescence quantitative PCR.

Results: Compared with the control group, the levels of serum sex hormones, fasting blood glucose, fasting insulin, and IR in the model group showed significant increases, and the levels of ANGPTL2, Foxol, and Akt in the ovarian tissue also showed significant increases. Compared with the PCOS group, the serum sex hormones, fasting blood glucose, fasting insulin, and IR of rats in the metformin group were significantly decreased, and the levels of ANGPTL2, Foxol, and Akt in ovarian tissues also showed significant decreases.

Conclusions: These findings suggest that ANGPTL2 might participate in the development of PCOS through the PI3K/Akt signaling pathway. Metformin improves IR by reducing the expression of ANGPTL2, thus improving the endocrine environment of PCOS and might change the disease outcome.
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http://dx.doi.org/10.1186/s12958-020-00651-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520960PMC
September 2020

Ablation of lncRNA MIAT mitigates high glucose-stimulated inflammation and apoptosis of podocyte via miR-130a-3p/TLR4 signaling axis.

Biochem Biophys Res Commun 2020 Dec 21;533(3):429-436. Epub 2020 Sep 21.

State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Chinese PLA General Hospital, Beijing, 100853, China. Electronic address:

Podocyte injury has been considered as a major contributor to the progression of diabetic nephropathy (DN). Long non-coding RNAs (lncRNAs) are being found to be involved in DN pathogenesis. The current research was designed to elucidate the potential role and latent molecular mechanism of long non-coding RNA MIAT in HG-induced podocyte injury. Our data demonstrated that MIAT expression was substantially elevated but miR-130a-3p was diminished in HG-challenged podocytes. Additionally, lack of MIAT mitigated HG-evoked inflammatory reaction in podocytes as evidenced by the diminished the release of inflammatory mediators TNF-α, IL-6 and IL-1β. Moreover, depletion of MIAT evidently amplified cell viability and alleviated HG-triggered apoptosis, reflected as the downregulation of Bax expression concomitant with the enhancement of Bcl-2 expression in HG-exposed podocytes. Mechanistically, MIAT effectively modulated TLR4 expression through acting as a competing endogenous sponge of miR-130a-3p, and TLR4 was confirmed as a specific target gene of miR-130a-3p. More importantly, the miR-130a-3p/TLR4 crosstalk contributed to the protective effect of MIAT knockdown on HG-provoked podocyte damage. Collectively, these findings highlighted that blocking MIAT/miR-130a-3p/TLR4 network play vital regulatory roles in mitigating HG-induced inflammation damage and apoptosis, thereby protecting podocyte from HG-stimulated injury, implying that MIAT might be a promising therapeutic strategy for developing effective treatments against DN progression.
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http://dx.doi.org/10.1016/j.bbrc.2020.09.034DOI Listing
December 2020

A Nickel- and Cerium-Doped Zeolite Composite: An Affordable Cathode Material for Biohydrogen Production in Microbial Electrolysis Cells.

Chempluschem 2020 10 23;85(10):2290-2297. Epub 2020 Sep 23.

College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan, 030024, P. R. China.

Microbial electrolysis cells (MECs) is one of the promising biohydrogen production technologies for which low-cost cathode materials are required and developed to propel the rapid development of MECs. Herein, the preparation of a low-cost Ce -Ni-Y composite is reported by using Y zeolite as carrier loaded with nickel (Ni) and cerium (Ce) as active components and its prominent electrochemical performance. The XPS analysis reveals that strong electronic interaction between Ni and Ce makes a great contribution to the electrochemical performance enhancement. The Ce -Ni-Y with a peak current density of 39.8 A⋅m in LSV, Tafel slope of 40.81 mV⋅dec , ECSA of 34.3 and hydrogen yield of 0.312±0.013 m ⋅m  d are significantly superior to that of its parent Ni-Y counterpart and rival the performance of commercially Pt/C, which renders it a very promising hydrogen evolution catalyst for MECs.
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http://dx.doi.org/10.1002/cplu.202000492DOI Listing
October 2020

Corrigendum to "Delivery of oncolytic adenovirus into the nucleus of tumorigenic cells by tumor microparticles for virotherapy" [Biomaterials 89 (2016) 56-66].

Biomaterials 2020 Dec 16;263:120372. Epub 2020 Sep 16.

National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing, 100005, China; Department of Biochemistry & Molecular Biology, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, 430030, China. Electronic address:

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http://dx.doi.org/10.1016/j.biomaterials.2020.120372DOI Listing
December 2020

Structurally-thrifty and visible-absorbing fluorophores.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jan 4;245:118907. Epub 2020 Sep 4.

State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. Electronic address:

Fluorophores with a minimal push-pull backbone are actively pursued due to their potentials in biological labelling. Herein a series of structurally-thrifty and visible-absorbing fluorophores (SDXs) were successfully constructed following the D'D-π-A design strategy, in which a secondary donor (D') was introduced in conjugation with the donor (D) to enhance its electron donating capability. For a very small scaffold, SDXs exhibit a surprisingly long-wavelength absorption band in the visible spectral range (λ = 420 nm) and a strong green fluorescence emission (λ = 530 nm) with a fluorescence quantum yield up to 0.84. Notably, fluorescence of SDXs was quenched in hydrogen-bonding solvents, e.g. MeOH and HO. This phenomenon renders SDXs feasibility for imaging of cellular non-hydrogen-bonding microenvironment, as demonstrated with BEAS-2B cells. These results proved that the D'D-π-A is a powerful design strategy to construct novel structurally-thrifty fluorophores.
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http://dx.doi.org/10.1016/j.saa.2020.118907DOI Listing
January 2021

The Molecular Adaptive Responses of Skeletal Muscle to High-Intensity Exercise/Training and Hypoxia.

Antioxidants (Basel) 2020 Jul 24;9(8). Epub 2020 Jul 24.

Institute for Health and Sport (iHeS), Victoria University, P.O. Box 14428, Melbourne 8001, Australia.

High-intensity exercise/training, especially interval exercise/training, has gained popularity in recent years. Hypoxic training was introduced to elite athletes half a century ago and has recently been adopted by the general public. In the current review, we have summarised the molecular adaptive responses of skeletal muscle to high-intensity exercise/training, focusing on mitochondrial biogenesis, angiogenesis, and muscle fibre composition. The literature suggests that (peroxisome proliferator-activated receptor gamma coactivator 1-alpha) PGC-1α, vascular endothelial growth factor (VEGF), and hypoxia-inducible factor 1-alpha (HIF1-α) might be the main mediators of skeletal muscle adaptations to high-intensity exercises in hypoxia. Exercise is known to be anti-inflammatory, while the effects of hypoxia on inflammatory signalling are more complex. The anti-inflammatory effects of a single session of exercise might result from the release of anti-inflammatory myokines and other cytokines, as well as the downregulation of Toll-like receptor signalling, while training-induced anti-inflammatory effects may be due to reductions in abdominal and visceral fat (which are main sources of pro-inflammatory cytokines). Hypoxia can lead to inflammation, and inflammation can result in tissue hypoxia. However, the hypoxic factor HIF1-α is essential for preventing excessive inflammation. Disease-induced hypoxia is related to an upregulation of inflammatory signalling, but the effects of exercise-induced hypoxia on inflammation are less conclusive. The effects of high-intensity exercise under hypoxia on skeletal muscle molecular adaptations and inflammatory signalling have not been fully explored and are worth investigating in future studies. Understanding these effects will lead to a more comprehensive scientific basis for maximising the benefits of high-intensity exercise.
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http://dx.doi.org/10.3390/antiox9080656DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464156PMC
July 2020

Porphyrin-Based Metal-Organic Framework Compounds as Promising Nanomedicines in Photodynamic Therapy.

ChemMedChem 2020 Oct 31;15(19):1766-1775. Epub 2020 Aug 31.

Institute of Pharmacy & Pharmacology, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, University of South China, Hengyang City, Hunan Province, 421001, China.

Porphyrin photosensitizers are widely used in photodynamic therapy (PDT) because of their unique diagnostic and therapeutic functions. However, many factors such as poor water solubility and instability of porphyrin compounds have limited their clinical application. Metal-organic frameworks (MOFs) have the beneficial characteristics of versatility, high porosity, and excellent biocompatibility. Porphyrin-MOF nanomaterials have attracted the attention of researchers because MOFs can effectively suppress the quenching caused by the self-aggregation of porphyrin compounds and promote drug delivery. This article reviews the latest applications of porphyrin-MOF nanomedicine in type II photodynamic therapy by increasing tumour cell oxygen concentration, depleting tumour cell functional molecules and releasing signal molecules. Current potential limitations and future applications are also emphasized and discussed herein.
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http://dx.doi.org/10.1002/cmdc.202000353DOI Listing
October 2020

Phoenixin-20 Ameliorates Lipopolysaccharide-Induced Activation of Microglial NLRP3 Inflammasome.

Neurotox Res 2020 Oct 10;38(3):785-792. Epub 2020 Jul 10.

Department of Neurology, First Affiliated Hospital, Hunan University of Medicine, Huaihua, No.225, Yushi Road, Hecheng District, Huaihua City, 418000, Hunan Province, China.

Injury associated with neuroinflammation has been linked with several kinds of neurodegenerative diseases. The activation of the NLRP3 inflammasome plays an important role in microglia-mediated inflammation. Phoenixin (PNX)-20 is a newly discovered neuropeptide with pleiotropic effects involved in the regulation of reproductive and cognitive function, depression, and food uptake. This study investigated whether PNX-20 possesses a protective effect against lipopolysaccharide (LPS)-induced activation of the NLRP3 inflammasome in microglia. Firstly, our results show that the PNX-20 treatment significantly prevented LPS-induced expression of NADPH oxidase 4 (NOX-4) and the generation of reactive oxygen species (ROS). Secondly, PNX-20 mitigated LPS-induced upregulation of TxNIP, an upstream regulator of NLRP3 inflammasome activation. Thirdly, further evaluation of the major components of the NLRP3 inflammasome revealed that PNX-20 inhibited LPS-mediated upregulation of NLRP3, ASC, and cleaved caspase-1 (P10). Notably, based on our results, the inhibitory effect of PNX-20 on the NLRP3 inflammasome results in the inhibition of IL-1β and IL-18 secretions. Finally, we found that PNX-20 ameliorated the reduction in SIRT1 expression induced by LPS. When microglial SIRT1 was inhibited by nicotine, PNX-20 lost its suppressive effect on the expression of NLRP3, ASC, and caspase-1, as well as the secretion of IL-1β and IL-18. As a result of these findings, we draw the conclusion that the neuroprotective effect of PNX-20 is dependent on SIRT1. Collectively, the study shows that PNX-20 has a regulatory effect via modulation of neuroinflammation.
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http://dx.doi.org/10.1007/s12640-020-00225-wDOI Listing
October 2020

Selective application of neuroendocrine markers in the diagnosis and treatment of small cell lung cancer.

Clin Chim Acta 2020 Oct 24;509:295-303. Epub 2020 Jun 24.

Department of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, China. Electronic address:

Aims: Here, we explored the potential application and selection of neuroendocrine biomarkers in the diagnosis and treatment of small cell lung cancer.

Methods: We retrospectively analyzed 118 patients with small cell lung cancer (SCLC), 166 patients with non-small cell lung cancer (NSCLC), 33 patients with benign lung disease (BLD), and 200 healthy individuals admitted to Zhejiang Provincial People's Hospital between January 1, 2015 and May 31, 2019. All the patients were newly diagnosed with either SCLC, NSCLC, or BLD and previously untreated. Peripheral blood levels of ProGRP, NSE, CEA, and CYFRA21-1 were analyzed during the follow-up treatment, and 2-fold upper limit of reference intervals were defined as effective elevation. We used paired results to analyze the diagnostic efficiency of proGRP and NSE on SCLC.

Results: In the 118 SCLC patients, proGRP levels were significantly higher compared with NSE levels. The diagnostic efficiencies of NSE and ProGRP for SCLC were 0.8554 and 0.9053, respectively. The combined diagnostic efficiency (0.9426) was higher relative to NSE, but there was no significant difference compared with proGRP. The effective elevation rate of proGRP was 45.3% higher than that of NSE in the limited stage of SCLC. In the extensive disease of SCLC patients, 70.7% cases had more than 10-fold increase in proGRP value, whereas 56.9% cases had less than 5-fold increase in NSE value. Compared with pre-treatment, the median concentrations of proGRP increased by 204.0% higher than that of NSE (71.3%) in the progressive group. Besides, the dynamic change in imaging characteristics and tumor size had a strong correlation with the levels of proGRP.

Significance: ProGRP is a reliable neuroendocrine biomarker in SCLC. The effective elevation of proGRP has a potential diagnostic and efficacy value in the evaluation of SCLC. However, the combined detection of proGRP and NSE does not significantly improve the diagnosis of lung cancer.
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http://dx.doi.org/10.1016/j.cca.2020.06.037DOI Listing
October 2020

Ofloxacin laden microemulsion contact lens to treat conjunctivitis.

J Biomater Sci Polym Ed 2020 08 21;31(12):1566-1579. Epub 2020 May 21.

Ophthalmology, Jinan Second People's Hospital, Jinan City, Shandong Province, China.

Currently, conjunctivitis is treated using ofloxacin eye drop solution, which shows low bioavailability and patient non-compliance. Ofloxacin loaded contact lens can be used to overcome the issues related to eye drop solutions (i.e. low bioavailability and frequent instillation). However, the conventional soaking method shows poor ofloxacin uptake and significantly affect the swelling and optical properties of the contact lenses. The current research investigates the effect of microemulsion on the ofloxacin uptake and its effect on the physical properties of the contact lens along with ofloxacin-release kinetics. In comparison to the conventional soaking method (Of-SM, ofloxacin-packaging solution), the ofloxacin loaded microemulsion-soaked contact lenses (Of-ME) showed improved drug uptake and physical properties of the contact lenses. The release data of Of-ME contact lenses showed 72-120 h release profile, while Of-SM contact lenses showed 24-60 h. The drug release data in the tear fluid (New Zealand rabbit's eye) showed high ofloxacin retention in comparison to the eye drop solution. The efficacy study in the rabbit model showed equivalent healing effect with the Of-ME contact lens in comparison to the frequent high dose eye drop therapy. Thus, the study demonstrates the application of microemulsion system to improve the ofloxacin loading capacity in the contact lens along with improvement in the physical properties to treat conjunctivitis.
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http://dx.doi.org/10.1080/09205063.2020.1764165DOI Listing
August 2020

Highly efficient electrosynthesis of hydrogen peroxide on a superhydrophobic three-phase interface by natural air diffusion.

Nat Commun 2020 04 7;11(1):1731. Epub 2020 Apr 7.

Key Laboratory of Pollution Process and Environmental Criteria, Ministry of Education, College of Environmental Science and Engineering, Nankai University, 300350, Tianjin, China.

Hydrogen peroxide (HO) synthesis by electrochemical oxygen reduction reaction has attracted great attention as a green substitute for anthraquinone process. However, low oxygen utilization efficiency (<1%) and high energy consumption remain obstacles. Herein we propose a superhydrophobic natural air diffusion electrode (NADE) to greatly improve the oxygen diffusion coefficient at the cathode about 5.7 times as compared to the normal gas diffusion electrode (GDE) system. NADE allows the oxygen to be naturally diffused to the reaction interface, eliminating the need to pump oxygen/air to overcome the resistance of the gas diffusion layer, resulting in fast HO production (101.67 mg h cm) with a high oxygen utilization efficiency (44.5%-64.9%). Long-term operation stability of NADE and its high current efficiency under high current density indicate great potential to replace normal GDE for HO electrosynthesis and environmental remediation on an industrial scale.
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http://dx.doi.org/10.1038/s41467-020-15597-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138826PMC
April 2020

Identification of Frataxin as a regulator of ferroptosis.

Redox Biol 2020 05 2;32:101483. Epub 2020 Mar 2.

Phase I Clinical Research Center, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, 310014, China; Clinical Research Institute, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, 310014, China; Bengbu Medical College, Bengbu, Anhui, 233000, China. Electronic address:

Ferroptosis is a newly discovered form of non-apoptotic regulated cell death and is characterized by iron-dependent and lipid peroxidation. Due to the enhanced dependence on iron in cancer cells, induction of ferroptosis is becoming a promising therapeutic strategy. However, the precise underlying molecular mechanism and regulation process of ferroptosis remains largely unknown. In the present study, we demonstrate that the protein Frataxin (FXN) is a key regulator of ferroptosis by modulating iron homeostasis and mitochondrial function. Suppression of FXN expression specifically repressed the proliferation, destroyed mitochondrial morphology, impeded Fe-S cluster assembly and activated iron starvation stress. Moreover, suppression of FXN expression significantly enhanced erastin-induced cell death through accelerating free iron accumulation, lipid peroxidation and resulted in dramatic mitochondria morphological damage including enhanced fragmentation and vanished cristae. In addition, this type of cell death was confirmed to be ferroptosis, since it could be pharmacologically restored by ferroptotic inhibitor Fer-1 or GSH, but not by inhibitors of apoptosis, necrosis. Vice versa, enforced expression of FXN blocked iron starvation response and erastin-induced ferroptosis. More importantly, pharmacological or genetic blocking the signal of iron starvation could completely restore the resistance to ferroptosis in FXN knockdown cells and xenograft graft in vivo. This paper suggests that FXN is a novel ferroptosis modulator, as well as a potential provided target to improve the antitumor activity based on ferroptosis.
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http://dx.doi.org/10.1016/j.redox.2020.101483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068686PMC
May 2020

Improving characteristics of biochar produced from collagen-containing solid wastes based on protease application in leather production.

Waste Manag 2020 Mar 5;105:531-539. Epub 2020 Mar 5.

Room L1.42 Gorlaeus Laboratory, Leiden University, the Netherlands. Electronic address:

Preparation of biochar from industrial solid wastes is receiving increasing attention in recent years. In this paper, alkaline protease, neutral protease and collagenolytic protease are used in preliminary steps of leather production, which are expected to replace the traditional chemical agents while preserving quality of the finished leather. The protease application has remarkable positive influence towards characteristics of biochar prepared by collagen-containing solid wastes produced in preliminary steps. The enzymatic action time should be more than 3 h for complete permeation and catalysis, and the diameters of treated collagen fibers were in the range of 10 to 20 nm. The micro-cracks occurring on collagen fibers would have an obviously impact on the formation of biochar. The application of proteases reduce the pollution of traditional production through replacing traditional polluted chemicals, and the characteristics of biochars are obviously improved with good surface area and high carbon content approximately 70%. Its surface area can reach 967 m/g. These biochars contain oxygen-containing functional groups, and the oxygen content of biochars are all over 20%. The enzyme application in leather production are effective to the properties of biochars prepared by collagen-containing solid wastes. This research can serve as a basis for the preparation of biochar derived from of natural bio-wastes thereby promoting the development of biomaterials.
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http://dx.doi.org/10.1016/j.wasman.2020.02.043DOI Listing
March 2020

Magnetically separable Fe-MIL-88B_NH carbonaceous nanocomposites for efficient removal of sulfamethoxazole from aqueous solutions.

J Colloid Interface Sci 2020 Jun 29;570:163-172. Epub 2020 Feb 29.

College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China; Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, China. Electronic address:

Extensive exposure to antibiotics could potentially be harmful to the environment and human health. The development of effective and convenient technologies to remove residual antibiotics from water is imperative. Herein, we successfully developed a facile method via pyrolysis of Fe-MIL-88B_NH to synthesize magnetic nanocomposites (MNC) as potential adsorbents, which exhibited cluster-shape structure and excellent magnetic response. Magnetic nanocomposites carbonized at 700 °C showed high efficiency for sulfamethoxazole (SMX) adsorption (73.53 mg/g). Some experimental conditions including solution pH, ionic strength, coexisting ions and SMX concentration were systematically investigated. The adsorption isotherm and kinetic followed Langmuir and the pseudo-second-order models, and the adsorption process was dependent on the solution pH. The adsorption mechanism hypothesis was pore filling effect, π-π EDA and electrostatic interactions. Moreover, MNC-700 exhibited good reusability and magnetic separation properties, being reused six times without significant loss in adsorption capacity.
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http://dx.doi.org/10.1016/j.jcis.2020.02.116DOI Listing
June 2020

Discovery of specific HDAC6 inhibitor with anti-metastatic effects in pancreatic cancer cells through virtual screening and biological evaluation.

Bioorg Chem 2020 04 20;97:103679. Epub 2020 Feb 20.

School of Life Sciences and Biopharmaceutical Science, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang 110016, PR China. Electronic address:

Histone deacetylase 6 (HDAC6) has been demonstrated to play a major role in cell motility and aggresome formation, and HDAC6 inhibition is therefore considered as a promising epigenetic strategy for cancer treatment. At present, only a minority of compounds have been reported as HDAC6 inhibitors, so specific HDAC6 inhibitors with safety profile need to be discovered urgently. In this paper, HDAC6 inhibitors with diverse structures were used to generate the pharmacophore model by ligand-based method, which contained two hydrogen bond acceptors and two hydrophobic groups. A combined virtual screening based on pharmacophore model and molecular docking was adopted to screen potential HDAC6 inhibitors. Subsequently, the HDAC6 inhibitory activity of the hit compounds were evaluated using an in vitro enzyme binding inhibition assay. The experimental results illustrated that cefoperazone sodium had the strongest inhibitory effect on HDAC6 among the six screened compounds, and its IC value was 8.59 ± 1.06 μM. Cefoperazone sodium significantly catalyzed the hyperacetylation of α-tubulin but not histone H3, proving that cefoperazone sodium was a selective inhibitor of HDAC6. Since the expression of HDAC6 plays an important role in cancer metastasis, the effects of cefoperazone sodium on migration and invasion of human pancreatic cancer PANC-1 cells were further investigated by wound healing and transwell chamber assays. It was found that cefoperazone sodium could evidently inhibit the migration and invasion of PANC-1 cells. Furthermore, the binding pattern of inhibitor at the active site of the crystal structure was revealed by molecular docking, providing a reference value for the structural design and optimization of HDAC6 inhibitors. This study provides a systematic virtual screening approach for discovering HDAC6 active inhibitors, and by which the specific effect of cefoperazone sodium against HDAC6 was found, suggesting its potential application on cancer therapy.
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http://dx.doi.org/10.1016/j.bioorg.2020.103679DOI Listing
April 2020

Electro-Fenton and photoelectro-Fenton degradation of sulfamethazine using an active gas diffusion electrode without aeration.

Chemosphere 2020 Jul 17;250:126177. Epub 2020 Feb 17.

Department of Chemical Sciences, University of Johannesburg, Doornfontein, South Africa; Centre for Nanomaterials Science Research, University of Johannesburg, South Africa.

A novel superhydrophobic gas diffusion electrode based on carbon black (CB)- polytetrafluoroethylene (PTFE) modified graphite felt cathode was prepared to increase oxygen mass transfer efficiency and produce hydrogen peroxide at the gas-liquid-solid three-phase interface without aeration. The gas diffusion electrode system was further tested for the degradation of sulfamethazine (SMT) by electro-Fenton (EF) and photoelectro-Fenton (PEF). In the EF process, SMT was removed effectively, but the mineralization degree was not high due to the generation of organic acids which were difficult to be further degraded. While in the PEF process, organic contaminant can be destroyed by the combined action of Fe/HO, UV/HO and UV radiation, and more efficient mineralization (>83.5%) at low current (50 mA) was attained, which might be attributed to the high HO utilization (70-90%), rapid regeneration of Fe and photolysis of intermediates. In addition, it was verified that the PEF system had a good adaptability to pH and pollutant concentration. Compared with aeration system, the use of this active gas diffusion cathode in electrochemical advanced oxidation processes significantly reduced energy consumption.
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http://dx.doi.org/10.1016/j.chemosphere.2020.126177DOI Listing
July 2020