Publications by authors named "Yanbin Wang"

197 Publications

Human Acellular Amniotic Matrix with Previously Seeded Umbilical Cord Mesenchymal Stem Cells Restores Endometrial Function in a Rat Model of Injury.

Mediators Inflamm 2021 3;2021:5573594. Epub 2021 Sep 3.

Peking University People's Hospital, Reproductive Medicine Center, Beijing 100044, China.

Background: Abnormal endometrial repair after injury results in the formation of intrauterine adhesions (IUA) and a thin endometrium, which are key causes for implantation failure and infertility. Stem cell transplantation offers a potential alternative for some cases of severe Asherman's syndrome that cannot be treated with surgery or hormonal therapy. Umbilical cord-derived mesenchymal stem cells (UCMSCs) have been reported to repair the damaged endometrium. However, there is no report on the effects of UCMSCs previously seeded on human acellular amniotic matrix (AAM) on endometrial injury.

Methods: Absolute ethanol was injected into rat uteri to damage the endometrium. UCMSCs previously seeded on AAM were surgically transplanted. Using a variety of methods, the treatment response was assessed by endometrial thickness, endometrial biomarker expression, endometrial receptivity, cell proliferation, and inflammatory factors.

Results: Endometrial thickness was markedly improved after UCMSC-AAM transplantation. The expression of endometrial biomarkers, namely, vimentin, cytokeratin, and integrin 3, in treated rats increased compared with untreated rats. In the UCMSC-AAM group, the VEGF expression decreased, whereas that of MMP9 increased compared with the injury group. Moreover, in the AAM group, the MMP9 expression increased. The expression of proinflammatory factors (IL-2, TNF, and IFN-) in the UCMSC-AAM group decreased compared with the untreated group, whereas the expression of anti-inflammatory factors (IL-4, IL-10) increased significantly.

Conclusions: UCMSC transplantation using AAM as the carrier can be applied to treat endometrial injury in rats. The successful preparation of lyophilized AAM provides the possibility of secondary infectious disease screening and amniotic matrix quality detection, followed by retrospective analysis. The UCMSC-AAM complex may promote the better application of UCMSCs on the treatment of injured endometrium.
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http://dx.doi.org/10.1155/2021/5573594DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438588PMC
September 2021

TMT-based quantitative proteomic analysis reveals the spleen regulatory network of dexamethasone-induced immune suppression in chicks.

J Proteomics 2021 Sep 18;248:104353. Epub 2021 Aug 18.

Henan Key laboratory for innovation and utilization of chicken germplasm resources, Zhengzhou 450046, China; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China. Electronic address:

Stress-induced immunosuppression is one of the most widespread problems in the poultry industry. Understanding the molecular regulatory mechanism of immunosuppression induced by stress in the chicken spleen would provide a scientific foundation for the prevention of stress reactions and antistress molecular breeding in poultry. To assess the protein expression profile of spleen tissue in a stress-included immunosuppression model, we performed a TMT-based proteomic analysis of chicken spleen tissue in a Dex-induced immunosuppression model (group C) and a control group (group A). We identified 590 differentially abundant proteins (DAPs) in chicken spleen tissue. These DAPs were significantly enriched in the following functional categories: ECM-receptor interaction, DNA replication, p53 signaling pathway, PI3K-Akt signaling pathway and NF-kappa B signaling pathway. Integrative analysis of the proteome and our previous transcriptome data revealed 62 DAPs showing correlations with the expression of their encoding mRNAs. Complementary proteome- and transcriptome-level analyses revealed a complex molecular network of stress-included immunosuppression. DPP4 and ALDH1A3 were the most significantly upregulated DAPs. GBP and OASL were identified as important nodes in the network related to stress-induced immunosuppression. The candidate genes identified in this study may be useful for the marker-based breeding of new chicken varieties with reduced stress levels. SIGNIFICANCE: This study provides a large amount of new information about the spleen proteome of the Dex-induced immunosuppression in chicks, as well as the correlation of transcriptome and proteome. Analysis of this resource has enabled us to examine mechanism of protein and transcript diversification, which expands the understanding of the complexity of the mechanism of stress-induced immunosuppression.
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http://dx.doi.org/10.1016/j.jprot.2021.104353DOI Listing
September 2021

Autophagy activation promotes the effect of iPSCs-derived NSCs on bladder function restoration after spinal cord injury.

Tissue Cell 2021 Oct 22;72:101596. Epub 2021 Jul 22.

Department of Orthopedics, Jiangnan Hospital Affiliated to Zhejiang Chinese Medical University, China.

The role of autophagy in the transplantation of induced pluripotent stem cells (iPSCs)-derived neural stem cells (NSCs) to treat spinal cord injury (SCI) and neurogenic bladder was investigated in this study. NSCs derived from human iPSCs were identified by and immunofluorescence assay. To clarify the role of autophagy, iPSCs were treated with either an autophagy inducer (rapamycin), or an autophagy inhibitor (chloroquine). Cell Counting kit-8 (CCK-8), western blot and flow cytometry were used to detect the effect of autophagy on the viability and differentiation of iPSCs. Sixty Wistar rats were selected to establish the SCI model and treated with iPSCs-derived NSCs transplantation. The effect of autophagy on the bladder function of rats with different treatments was evaluated by Basso, Beattie, and Bresnahan (BBB) score, bladder function score, bladder weight measurement, Hematoxylin & Eosin (H&E) staining, and Masson staining. The results of in vitro experiment showed that rapamycin enhanced the cell activity of iPSCs, increased the number of nestin positive cells, up-regulated Beclin-1 and LC3BI/II expressions, and down-regulated p62 expression. And the results of in vivo experiment showed that rapamycin improved exercise ability and bladder function, partially restored bladder weight, and significantly reduced bladder tissue damage in SCI rats. However, chloroquine showed the opposite results. The differentiation of iPSCs into NSCs could be promoted by induced autophagy, while neurogenic bladder of SCI was restored by autophagy activation.
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http://dx.doi.org/10.1016/j.tice.2021.101596DOI Listing
October 2021

The chicken pan-genome reveals gene content variation and a promoter region deletion in IGF2BP1 affecting body size.

Mol Biol Evol 2021 Jul 30. Epub 2021 Jul 30.

College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.

Domestication and breeding have reshaped the genomic architecture of chicken, but the retention and loss of genomic elements during these evolutionary processes remain unclear. We present the first chicken pan-genome constructed using 664 individuals, which identified an additional ∼66.5 Mb sequences that are absent from the reference genome (GRCg6a). The constructed pan-genome encoded 20,491 predicated protein-coding genes, of which higher expression level are observed in conserved genes relative to dispensable genes. Presence/absence variation (PAV) analyses demonstrated that gene PAV in chicken was shaped by selection, genetic drift, and hybridization. PAV-based GWAS identified numerous candidate mutations related to growth, carcass composition, meat quality, or physiological traits. Among them, a deletion in the promoter region of IGF2BP1 affecting chicken body size is reported, which is supported by functional studies and extra samples. This is the first time to report the causal variant of chicken body size QTL located at chromosome 27 which was repeatedly reported. Therefore, the chicken pan-genome is a useful resource for biological discovery and breeding. It improves our understanding of chicken genome diversity and provides materials to unveil the evolution history of chicken domestication.
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http://dx.doi.org/10.1093/molbev/msab231DOI Listing
July 2021

Core-shell shaped [email protected] microspheres from prussian blue analogues for high performance asymmetric supercapacitors.

Nanotechnology 2021 Aug 13;32(44). Epub 2021 Aug 13.

College of Chemical Engineering, Lanzhou University of Arts and Science, Lanzhou, 730000, People's Republic of China.

Recently, prussian blue analogues (PBAs), as the most classical class of metal-organic frameworks, have been widely studied by scientists. Nevertheless, the inferior conductivity of PBAs restricts the application in supercapacitors. In this work, nickel cobalt hexacyanoferrate (NiCoHCF) had been produced via a simple co-precipitation approach and coated with polypyrrole on its surface. The conductivity of PBAs was improved by the polypyrrole coating. The [email protected] microspheres were demonstrated to the outstanding specific capacity of 82 mAh gat 1 A g. After 3000 cycles, the [email protected] microspheres had a long cycle life and 86% specific capacity retention rate at 5 A g. Additionally, it was coupled with activated carbon to build high performance asymmetric supercapacitor ([email protected]//AC), which displayed a high energy density of 21.7 Wh kgat the power density of 888 W kgand good cycle stability after 5000 cycles (a capacity retention rate of 85.2%). What is more, the results reveal that the [email protected] microspheresare a prospective candidate for exceptional energy storage devices.
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http://dx.doi.org/10.1088/1361-6528/ac17c2DOI Listing
August 2021

Monitoring Antihypertensive Medication Adherence by LC-MS/MS: Method Establishment and Clinical Application.

J Cardiovasc Pharmacol 2021 Jul 9. Epub 2021 Jul 9.

Jiangsu Province Hospital, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China, 210029 Fu Foundation School of Engineering and Applied Science, Columbia University, NY, USA, 10027 Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, Shanxi, China, 030009 Department of Cardiology, East Hospital Affiliated Tongji University, Shanghai, China, 200120 Frontage Labs, Shanghai, 201203 Hangzhou Calibra Diagnostic Ltd., Hangzhou, China, 310030 Division of Cardiology, Department of Medicine, College of Physicians & Surgeons, Columbia University, NY, USA, 10032.

Abstract: Proper medication compliance is critical for the integrity of clinical practice, directly related to the success of clinical trials to evaluate both pharmacological and device-based therapies. Here, we established a liquid chromatography tandem mass spectrometry (LC-MS/MS) method to accurately detect 55 chemical entities in human urine sample, which accounting for the most commonly used 172 antihypertensive drugs in China. The established method had good accuracy, intra-day and inter-day precision for all analyses in both bench tests and validated in 21 hospitalized patients. We utilized this method to monitor and ensure drug compliance, and exclude the inferring impacts of medication compliance as a key confounder for our pivotal trial of a catheter-based, renal mapping and selective renal denervation to treat hypertension. It is found that in the urine samples from 92 consecutive subjects, 85 subjects (92.4%) were consistent with their prescriptions after 28 days run-in periods, 90 (97.8%) and 85 (95.5%) patients completely complied with their medications during the 3-month and 6-month follow-up period, respectively. Thus, using LC-MS/MS method with specificity, accuracy and precision, we ensured drug compliance of patients, excluded the key confounder of drug interferences and ensured the quality of our device-based clinical trial for treatment of hypertension.
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http://dx.doi.org/10.1097/FJC.0000000000001105DOI Listing
July 2021

Acute-on-chronic Liver Failure in a Patient with : A Case Report.

J Clin Transl Hepatol 2021 Jun 30;9(3):447-451. Epub 2021 Mar 30.

Center of Liver Diseases, Capital Medical University Affiliated to Beijing Ditan Hospital, Beijing, China.

Acute-on-chronic liver failure (ACLF) is a risk factor for fungal infection. Endogenous fungal endophthalmitis is a serious, sight-threatening disease. Common causes include immunocompromised state and intravenous drug use, permitting opportunistic pathogens to reach the eye through the blood stream. We report a case of in a 47 year-old woman who was admitted to our hospital with ACLF and poorly controlled diabetes. In addition, she was treated with glucocorticoids due to severe jaundice. After treatment for ACLF, the patient experienced fever with blurred vision in the left eye and was diagnosed with candidemia, endogenous Candida endophthalmitis in the left eye, and chorioretinitis in the right eye. Systemic and topical antifungal treatment was administered based on the positive test in intraocular fluid using second-generation sequencing. The patient underwent vitrectomy in the left eye and was confirmed in vitreous cultures. Follow-up visit, at 6 weeks after the operation, showed only light perception in the left eye and stable visual acuity in the right eye. Physicians should be aware of endogenous fungal endophthalmitis in patients with ACLF, especially those with infection, a history of glucocorticoid use, and diabetes. A dilated retinal examination should be performed by an ophthalmologist if ACLF patients develop fever and fungal infection.
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http://dx.doi.org/10.14218/JCTH.2020.00092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237134PMC
June 2021

Novel multi-stimuli-responsive supramolecular gel based on quinoline for the fluorescence ultrasensitive detection of Feand Cu.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Nov 11;261:120078. Epub 2021 Jun 11.

College of Chemical Engineering, Lanzhou University of Arts and Science, Lanzhou 730000, People's Republic of China. Electronic address:

A novel gelator molecular based on quinolone (MN) has been successfully designed and synthesized. The gelator MN could self-assemble to form a supramolecular gel (OMN), which showed obvious aggregation-induced emission (AIE) in iso-Propyl alcohol (i-PrOH). Furthermore, the supramolecular organogel OMN realized ultrasensitive detection of Fe and Cu in aqueous medium and fluorescent quenching at 427 nm. The sensing mechanism between supramolecular gel and metal ions was fully investigated via FE-SEM, FT-IR, XRD and XPS. Meanwhile, a thin film based on responsive supramolecular gel OMN was prepared, which could be used as multi-stimuli-responsive fluorescent display materials for the detection of Fe and Cu.
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http://dx.doi.org/10.1016/j.saa.2021.120078DOI Listing
November 2021

Effect of a "Dual Trigger" Using a GnRH Agonist and hCG on the Cumulative Live-Birth Rate for Normal Responders in GnRH-Antagonist Cycles.

Front Med (Lausanne) 2021 25;8:683210. Epub 2021 May 25.

Reproductive Center of Peking University Peoples' Hospital, Beijing, China.

"Dual triggering" for final oocyte maturation using a combination of a gonadotropin-releasing hormone agonist (GnRHa) and human chorionic gonadotropin (hCG) can improve clinical outcomes in high responders during fertilization-intracytoplasmic sperm injection (IVF-ICSI) GnRH-antagonist cycles. However, whether this dual trigger is also beneficial to normal responders is not known. We retrospectively analyzed the data generated from 469 normal responders from 1 January to 31 December 2017. The final oocyte maturation was undertaken with a dual trigger with a GnRHa combined with hCG ( = 270) or hCG alone ( = 199). Patients were followed up for 3 years. The cumulative live-birth rate was calculated as the first live birth achieved after all cycles having an embryo transfer (cycles using fresh embryos and frozen-thawed embryos) among both groups. Women in the dual-trigger group achieved a slightly higher number of oocytes retrieved (11.24 vs. 10.24), higher number of two-pronuclear (2PN) embryos (8.37 vs. 7.67) and a higher number of embryos available (4.45 vs. 4.03). However, the cumulative live-birth rate and the all-inclusive success rate for assisted reproductive technology was similar between the two groups (54.07 vs. 59.30%). We showed that a dual trigger was not superior to a hCG-alone trigger for normal responders in GnRH-antagonist cycles in terms of the cumulative live-birth rate.
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http://dx.doi.org/10.3389/fmed.2021.683210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185054PMC
May 2021

Global investigation of estrogen-responsive genes regulating lipid metabolism in the liver of laying hens.

BMC Genomics 2021 Jun 9;22(1):428. Epub 2021 Jun 9.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.

Background: Estrogen plays an essential role in female development and reproductive function. In chickens, estrogen is critical for lipid metabolism in the liver. The regulatory molecular network of estrogen in chicken liver is poorly understood. To identify estrogen-responsive genes and estrogen functional sites on a genome-wide scale, we determined expression profiles of mRNAs, lncRNAs, and miRNAs in estrogen-treated ((17β-estradiol)) and control chicken livers using RNA-Sequencing (RNA-Seq) and studied the estrogen receptor α binding sites by ChIP-Sequencing (ChIP-Seq).

Results: We identified a total of 990 estrogen-responsive genes, including 962 protein-coding genes, 11 miRNAs, and 17 lncRNAs. Functional enrichment analyses showed that the estrogen-responsive genes were highly enriched in lipid metabolism and biological processes. Integrated analysis of the data of RNA-Seq and ChIP-Seq, identified 191 genes directly targeted by estrogen, including 185 protein-coding genes, 4 miRNAs, and 2 lncRNAs. In vivo and in vitro experiments showed that estrogen decreased the mRNA expression of PPARGC1B, which had been reported to be linked with lipid metabolism, by directly increasing the expression of miR-144-3p.

Conclusions: These results increase our understanding of the functional network of estrogen in chicken liver and also reveal aspects of the molecular mechanism of estrogen-related lipid metabolism.
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http://dx.doi.org/10.1186/s12864-021-07679-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190866PMC
June 2021

Isolation, purification and identification of biological compounds from Beauveria sp. and their evaluation as insecticidal effectiveness against Bemisia tabaci.

Sci Rep 2021 Jun 8;11(1):12020. Epub 2021 Jun 8.

College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China.

Bemisia tabaci is one of the most notorious agricultural pests in the world. A vicious circle among insect resistance, dose increased, environment and human body impaired as the overuse of synthetic pesticides are becoming increasingly evident. Entomopathogenic Beauveria sp. is known as an effective natural enemy to control B. tabaci. Therefore, this study aimed to purify and identify the biological compounds from Beauveria sp. LY2 via extensive chromatographic techniques, NMR and MS and evaluated for their insecticidal activities against B. tabaci via contact and feeding assay. The outcome identified that one new cerebroside, cerebroside F (1), nine known compounds, cerebroside B (2), bassiatin (3), methyl 1,4-dihydro-4-oxo-2-quinolinecarboxylate (4), cerevisterol (5), 9-hydroxycerevisterol (6), 6-dehydrocerevisterol (7), (22E,24R)-ergosta-8(14),22-diene-3β,5α,6β,7α-tetrol (8), melithasterol B (9) and ergosterol peroxide (10) were isolated. Among the known compounds, methyl 1,4-dihydro-4-oxo- 2-quinolinecarboxylate (4) was isolated from natural origin for the first time. It is demonstrable from the results that compounds 3, 4 and 7 strongly featured insecticidal activities against B. tabaci, being the LC value as 10.59, 19.05, 26.59 μg/mL respectively in contact as well as 11.42, 5.66, 5.65 μg/mL respectively in feeding experiment. Moreover, no adverse effect on plant growth/height or phytotoxicity was observed on pepper, cucumber, tomato and cotton. The data from the current study has provided the foundation for the use of newly purified compounds against Bemisia tabaci as an alternative to synthetic chemical compounds.
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http://dx.doi.org/10.1038/s41598-021-91574-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187347PMC
June 2021

Transvesical versus extravesical approach to laparoscopic posthysterectomy vesicovaginal fistula repair: A retrospective study from two medical centers.

Neurourol Urodyn 2021 08 31;40(6):1593-1599. Epub 2021 May 31.

Department of Urology, Nanchang University, Nanchang, Jiangxi, China.

Background: The controversy on the best surgical approach for vesicovaginal fistula (VVF) repair remains due to the scarcity of high-level evidences. We aim to analyze the efficacy and safety of the laparoscopic transvesical (LT) and laparoscopic extravesical (LE) approaches to posthysterectomy VVF (PH-VVF).

Methods: Data of 64 patients with PH-VVFs who were laparoscopicly treated in the First Affiliated Hospital of Nanchang University and the Hangzhou Third Hospital from January 2011 to November 2019 were retrospectively collected. The operative time (OT), estimated bleeding volume (EBV), postoperative bladder function and complications, hospital stay length (HSL), surgery success rate, and recurrence were compared between the two groups.

Results: In all, there were no significant differences in baseline characteristics between those two arms. Both approaches were successfully performed without open conversion in either group. The LT group was significantly corrected with a shorter mean OT than the LE group (p < 0.001), regardless of the fistula's position. No significant differences existed in the mean EBV and HSL between the two interventions (p = 0.136 and p = 0.210, respectively). The tendency of postoperative complications and success rates of surgery were also comparable in both groups. The patients in the LT group had similar bladder functions to those in LE group. The recurrence occurred in one patient in each arm during the follow-up periods of 12-36 months.

Conclusions: The LT approach is significantly related to a shorter OT than the LE approach without compromising the safety and success rates in repairing PH-VVF.
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http://dx.doi.org/10.1002/nau.24724DOI Listing
August 2021

Plastic Deformation and Strengthening Mechanisms of Nanopolycrystalline Diamond.

ACS Nano 2021 May 30;15(5):8283-8294. Epub 2021 Apr 30.

Geodynamics Research Center, Ehime University, Matsuyama, Ehime 790-8577, Japan.

Bulk nanopolycrystalline diamond (NPD) samples were deformed plastically within the diamond stability field up to 14 GPa and above 1473 K. Macroscopic differential stress Δσ was determined on the basis of the distortion of the 111 Debye ring using synchrotron X-ray diffraction. Up to ∼5(2)% strain, Debye ring distortion can be satisfactorily described by lattice strain theories as an ellipse. Beyond ∼5(2)% strain, lattice spacing along the Δσ direction becomes saturated and remains constant with further deformation. Transmission electron microscopy on as-synthesized NPD shows well-bonded grain boundaries with no free dislocations within the grains. Deformed samples also contain very few free dislocations, while density of {111} twins increases with plastic strain. Individual grains display complex contrast, exhibiting increasing misorientation with deformation according electron diffraction. Thus, NPD does not deform by dislocation slip, which is the dominated mechanism in conventional polycrystalline diamond composites (PCDCs, grain size >1 μm). The nonelliptical Debye ring distortion is modeled by nucleating dislocations or their dissociated partials gliding in the {111} planes to produce deformation twinning. With increasing strain up to ∼5(2)%, strength increases rapidly to ∼20(1) GPa, where reaches saturation. Strength beyond the saturation shows a weak dependence on strain, reaching ∼22(1) GPa at >10% strain. Overall, the strength is ∼2-3 times that of conventional PCDCs. Combined with molecular dynamics simulations and lattice rotation theory, we conclude that the rapid rise of strength with strain is due to defect-source strengthening, whereas further deformation is dominated by nanotwinning and lattice rotation.
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http://dx.doi.org/10.1021/acsnano.0c08737DOI Listing
May 2021

Characteristics and expression profiles of circRNAs during abdominal adipose tissue development in Chinese Gushi chickens.

PLoS One 2021 15;16(4):e0249288. Epub 2021 Apr 15.

College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan Province, P.R. China.

Circular RNAs (circRNAs) play important roles in adipogenesis. However, studies on circRNA expression profiles associated with the development of abdominal adipose tissue are lacking in chickens. In this study, 12 cDNA libraries were constructed from the abdominal adipose tissue of Chinese domestic Gushi chickens at 6, 14, 22, and 30 weeks. A total of 1,766 circRNAs were identified by Illumina HiSeq 2500 sequencing. These circRNAs were primarily distributed on chr1 through chr10 and sex chromosomes, and 84.95% of the circRNAs were from gene exons. Bioinformatic analysis showed that each circRNA has 35 miRNA binding sites on average, and 62.71% have internal ribosome entry site (IRES) elements. Meanwhile, these circRNAs were primarily concentrated in TPM < 0.1 and TPM > 60, and their numbers accounted for 18.90% and 80.51%, respectively, exhibiting specific expression patterns in chicken abdominal adipose tissue. In addition, 275 differentially expressed (DE) circRNAs were identified by comparison analysis. Functional enrichment analysis showed that the parental genes of DE circRNAs were primarily involved in biological processes and pathways related to lipid metabolism, such as regulation of fat cell differentiation, fatty acid homeostasis, and triglyceride homeostasis, as well as fatty acid biosynthesis, fatty acid metabolism, and glycerolipid metabolism. Furthermore, ceRNA regulatory networks related to abdominal adipose development were constructed. The results of this study indicated that circRNAs can regulate lipid metabolism, adipocyte proliferation and differentiation, and cell junctions during abdominal adipose tissue development in chickens through complex ceRNA networks between circRNAs, miRNAs, genes, and pathways. The results of this study may help to expand the number of known circRNAs in abdominal adipose tissue and provide a valuable resource for further research on the function of circRNAs in chicken abdominal adipose tissue.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249288PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049301PMC
April 2021

Genetic polymorphisms of metabolic enzyme genes associated with leukocyte mitochondrial DNA copy number in PAHs exposure workers.

Cancer Rep (Hoboken) 2021 Aug 31;4(4):e1361. Epub 2021 Mar 31.

Department of Occupational Health and Occupational Diseases, College of Public Health, Zhengzhou University, Zhengzhou, China.

Background: Polycyclic aromatic hydrocarbons (PAHs) exposure had been reported to be a risk factor of mtDNAcn in our early study. However, the effect of metabolic enzymes' genetic polymorphisms on mtDNAcn in PAHs-Exposure workers has not been fully evaluated.

Aim: The aim of the study was to explore the effect of metabolic enzymes' genetic polymorphisms on mtDNAcn in PAHs-Exposure.

Methods And Results: We investigated the effects of metabolic enzymes' genetic polymorphisms on mtDNAcn among 544 coke oven workers and 238 office staffs. The mtDNAcn of peripheral blood leukocytes was measured using the Real-time quantitative polymerase chain reaction (PCR) method. PCR and restriction fragment length was used to detect five polymorphisms in GSTT1, GSTM1, GSTP1 rs1695, CYP2E1 rs6413432, and CYP2E1 rs3813867. The mtDNAcn in peripheral blood leukocytes was significantly lower in the exposure group than that in the control group (p < .001). The 1-OHPYR had an increasing trend with the genotypes AA→AG → GG of GSTP1 rs1695 in the control group. Generalized linear model indicated that the influencing factors of mtDNAcn were PAHs-exposure [β (95% CI) = -0.420 (-0.469, -0.372), p < .001], male [β (95% CI) = -0.058 (-0.103, -0.012), p = .013], and AA genotype for GSTP1 rs1695 [β (95% CI) = -0.051 (-0.095, -0.008), p = .020].

Conclusion: The individuals carrying the AA genotype of GSTP1 rs1695 may have a lower mtDNAcn due to their weaker detoxification of PAHs.
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http://dx.doi.org/10.1002/cnr2.1361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388165PMC
August 2021

The prevalence of antibodies to SARS-CoV-2 among blood donors in China.

Nat Commun 2021 03 2;12(1):1383. Epub 2021 Mar 2.

National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, P.R. China.

In this study, we investigate the seroprevalence of SARS-CoV-2 antibodies among blood donors in the cities of Wuhan, Shenzhen, and Shijiazhuang in China. From January to April 2020, 38,144 healthy blood donors in the three cities were tested for total antibody against SARS-CoV-2 followed by pseudotype SARS-CoV-2 neutralization tests, IgG, and IgM antibody testing. Finally, a total of 398 donors were confirmed positive. The age- and sex-standardized SARS-CoV-2 seroprevalence among 18-60 year-old adults (18-65 year-old in Shenzhen) was 2.66% (95% CI: 2.24%-3.07%) in Wuhan, 0.033% (95% CI: 0.0029%-0.267%) in Shenzhen, and 0.0028% (95% CI: 0.0001%-0.158%) in Shijiazhuang, respectively. Female sex and older-age were identified to be independent risk factors for SARS-CoV-2 seropositivity among blood donors in Wuhan. As most of the population of China remained uninfected during the early wave of the COVID-19 pandemic, effective public health measures are still certainly required to block viral spread before a vaccine is widely available.
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http://dx.doi.org/10.1038/s41467-021-21503-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925561PMC
March 2021

CRISPR/Cas9-Engineered Universal CD19/CD22 Dual-Targeted CAR-T Cell Therapy for Relapsed/Refractory B-cell Acute Lymphoblastic Leukemia.

Clin Cancer Res 2021 May 24;27(10):2764-2772. Epub 2021 Feb 24.

Bone Marrow Transplantation Center, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, P.R. China.

Purpose: Autologous chimeric antigen receptor T (CAR-T) cell therapy is an effective treatment for relapsed/refractory acute lymphoblastic leukemia (r/r ALL). However, certain characteristics of autologous CAR-T cells can delay treatment availability. Relapse caused by antigen escape after single-targeted CAR-T therapy is another issue. Therefore, we aim to develop CRISPR-edited universal off-the-shelf CD19/CD22 dual-targeted CAR-T cells as a novel therapy for r/r ALL.

Patients And Methods: In this open-label dose-escalation phase I study, universal CD19/CD22-targeting CAR-T cells (CTA101) with a CRISPR/Cas9-disrupted region and gene to avoid host immune-mediated rejection were infused in patients with r/r ALL. Safety, efficacy, and CTA101 cellular kinetics were evaluated.

Results: CRISPR/Cas9 technology mediated highly efficient, high-fidelity gene editing and production of universal CAR-T cells. No gene editing-associated genotoxicity or chromosomal translocation was observed. Six patients received CTA101 infusions at doses of 1 (3 patients) and 3 (3 patients) × 10 CAR T cells/kg body weight. Cytokine release syndrome occurred in all patients. No dose-limiting toxicity, GvHD, neurotoxicity, or genome editing-associated adverse events have occurred to date. The complete remission (CR) rate was 83.3% on day 28 after CTA101 infusion. With a median follow-up of 4.3 months, 3 of the 5 patients who achieved CR or CR with incomplete hematologic recovery (CR/CRi) remained minimal residual disease (MRD) negative.

Conclusions: CRISPR/Cas9-engineered universal CD19/CD22 CAR-T cells exhibited a manageable safety profile and prominent antileukemia activity. Universal dual-targeted CAR-T cell therapy may offer an alternative therapy for patients with r/r ALL.
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http://dx.doi.org/10.1158/1078-0432.CCR-20-3863DOI Listing
May 2021

HRV16 Infection Induces Changes in the Expression of Multiple piRNAs.

Virol Sin 2021 Aug 22;36(4):736-745. Epub 2021 Feb 22.

State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.

Human rhinovirus (HRV) is one of the most important cold-causing pathogens in humans. Piwi-interacting RNAs (piRNAs) are a recently discovered class of small non-coding RNAs whose best-understood function is to repress mobile element (ME) activity in animal germline. However, the profile of human/host piRNA during HRV infection is largely unknown. Here we performed high-throughput sequencing of piRNAs from H1-HeLa cells infected with HRV16 at 12 h, 24 h, and 36 h. The results showed that 22,151,664, 24,362,486 and 22,726,546 piRNAs displayed differential expression after HRV16 infection for three time points. A significant differential expression of 21 piRNAs was found in all time points and further verified by RT-qPCR, including 7 known piRNAs and 14 newly found piRNAs. In addition, piRNA prediction was performed on Piano using the SVM algorithm and transposon information. It found that novel_pir78110, novel_pir78107, novel_pir78097, novel_pir78094 and novel_pir76584 are associated with the DNA/hobo of Drosophila, Ac of maize and Tam3 of snapdragon (hAT)-Charlie transposon. The novel_pir97924, novel_pir105705 and novel_pir105700 recognize long interspersed nuclear elements 1 (LINE-1). The novel_pir33182 and novel_pir46604 are related to the long terminal repeat (LTR)/(Endogenous Retrovirus1) ERV1 repetitive element. The novel_pir73855 is related to the LTR/ERVK repetitive element. Both novel_pir70108 and novel_pir70106 are associated with the LTR/ERVL-MaLR repetitive element. The novel_pir15900 is associated with the DNA/hAT-Tip100 repetitive element. Overall, our results indicated that rhinovirus infection could reduce the expression of some piRNAs to facilitate upregulation of LINE-1 transcription or retrotransposons' expression, which is helpful to further explore the mechanism of rhinovirus infection.
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http://dx.doi.org/10.1007/s12250-021-00344-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379331PMC
August 2021

Evolution, Expression Profile, Regulatory Mechanism, and Functional Verification of EBP-Like Gene in Cholesterol Biosynthetic Process in Chickens (Gallus Gallus).

Front Genet 2020 14;11:587546. Epub 2021 Jan 14.

College of Animal Science, Henan Agricultural University, Zhengzhou, China.

The emopamil binding protein (EBP) is an important enzyme participating in the final steps of cholesterol biosynthesis in mammals. A predictive gene , which encodes the protein with a high identity to human EBP, was found in chicken genome. No regulatory mechanisms and biological functions of have been characterized in chickens. In the present study, the coding sequence of was cloned, the phylogenetic trees of EBP/EBP-like were constructed and the genomic synteny of was analyzed. The regulatory mechanism of were explored with and experiments. The biological functions of in liver cholesterol biosynthetic were examined by using gain- or loss-of-function strategies. The results showed that chicken gene was originated from a common ancestral with Japanese quail gene, and was relatively conservative with gene among different species. The gene was highly expressed in liver, its expression level was significantly increased in peak-laying stage, and was upregulated by estrogen. Inhibition of the mRNA expression could restrain the expressions of downstream genes (, , and ) in the cholesterol synthetic pathway, therefore downregulate the liver intracellular T-CHO level. In conclusion, as substitute of gene in chickens, plays a vital role in the process of chicken liver cholesterol synthesis. This research provides a basis for revealing the molecular regulatory mechanism of cholesterol synthesis in birds, contributes insights into the improvement of the growth and development, laying performance and egg quality in poultry.
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http://dx.doi.org/10.3389/fgene.2020.587546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841431PMC
January 2021

Estimations of benchmark dose for urinary metabolites of coke oven emissions among workers.

Environ Pollut 2021 Jan 5;273:116434. Epub 2021 Jan 5.

Department of Occupational Health and Occupational Diseases, College of Public Health, Zhengzhou University, Zhengzhou, 450001, China; The Key Laboratory of Nanomedicine and Health Inspection of Zhengzhou, Zhengzhou, 450001, China. Electronic address:

Coke oven emissions (COEs), usually composed of polycyclic aromatic hydrocarbons (PAHs) and so on, may alter the relative telomere length of exposed workers and have been linked with adverse health events. However, the relevant biological exposure limits of COEs exposure has not been evaluated from telomere damage. The purpose of this study is to estimate benchmark dose (BMD) of urinary PAHs metabolites from COEs exposure based on telomere damage with RTL as a biomarker. A total of 544 exposed workers and 238 controls were recruited for participation. High-performance liquid chromatography and qPCR were used to detect concentrations of urinary mono-hydroxylated PAHs and relative telomere length in peripheral blood leukocytes for all subjects. The benchmark dose approach was used to estimate benchmark dose (BMD) and its lower 95% confidence limit (BMDL) of urinary OH-PAHs of COEs exposure based on telomere damage. Our results showed that telomere length in the exposure group (0.75 (0.51, 1.08)) was shorter than that in the control group (1.05 (0.76,1.44))(P < 0.05), and a dose-response relationship was shown between telomere damage and both 1-hydroxypyrene and 3-hydroxyphenanthrene in urine. The BMDL of urinary 1-hydroxypyrene from the optimal model for telomere damage was 1.96, 0.40, and 1.01 (μmol/mol creatinine) for the total, males, and females group, respectively. For 3-hydroxyphenanthrene, the BMDL was 0.94, 0.33, and 0.49 (μmol/mol creatinine) for the total, males, and females. These results contribute to our understanding of telomere damage induced by COEs exposure and provide a reference for setting potential biological exposure limits.
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http://dx.doi.org/10.1016/j.envpol.2021.116434DOI Listing
January 2021

Riverside Greenway in Urban Environment: Residents' Perception and Use of Greenways along the Huangpu River in Shanghai, China.

Int J Environ Res Public Health 2021 01 27;18(3). Epub 2021 Jan 27.

Shanghai Business School, College of Business Administration, Shanghai 200235, China.

Urban greenways improve green coverage rates in urban environments and transform these environments in a people-oriented manner. This study adopted semantic differential (SD) methods and an importance-performance analysis (IPA) model to evaluate resident perceptions and preferences of riverside greenways. A survey of 588 residents was conducted on typical natural greenways, built greenways, and mixed greenways along the Huangpu River in Shanghai. The results showed that resident perceptions of style, space, and distance differed markedly, whereas their perceptions of environmental and psychological characteristics were relatively similar. There were strong correlations between residents' characteristics and their perceptions, especially for their perceptions of greenway style, sense of order, and distance from the river. By comparison, most residents preferred mixed greenways. Additionally, respondents from areas with natural and mixed greenways believed that they benefited, whereas those from areas with built greenways displayed a potential sense of deprivation. The results of IPA analysis provide further support for the above conclusions. As a whole, the relatively simple methods demonstrated here could be useful to quantitatively analyze the subjective perceptions of urban residents.
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http://dx.doi.org/10.3390/ijerph18031120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908429PMC
January 2021

The roles of PAD2- and PAD4-mediated protein citrullination catalysis in cancers.

Int J Cancer 2021 01 8;148(2):267-276. Epub 2020 Aug 8.

Hangzhou Medical College, Hangzhou, Zhejiang, China.

Peptidylarginine deiminases (PADs) catalyze the conversion of arginine residues to citrulline residues on target proteins in the presence of calcium ions. This elaborate type of posttranslational modification is termed citrullination. PADs may regulate gene transcriptional activity via histone citrullination. There has been an increasing appreciation for the roles of PADs in a wide variety of biological processes. In this review article, we summarize recent evidence indicating that PADs and citrullinated proteins are involved in several physiological and pathological processes related to cancer. Of particular interest is that PAD2 and PAD4 exhibit characteristic expression levels, activities and specific biological effects in diverse types of cancer. We also list several PAD inhibitors, propose the possible mechanisms underlying the biological actions of PAD-mediated protein citrullination in experimental models and discuss the potential therapeutic value of PADs and their inhibitors for disease diagnosis and treatment.
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http://dx.doi.org/10.1002/ijc.33205DOI Listing
January 2021

Structural characteristics and enhanced biological activities of partially degraded arabinogalactan from larch sawdust.

Int J Biol Macromol 2021 Feb 11;171:550-559. Epub 2021 Jan 11.

The Key Laboratory of Biochemical Utilization of Zhejiang Province, Key Laboratory of State Forest Food Resources Utilization and Quality Control, Zhejiang Academy of Forestry, Hangzhou 310023, China. Electronic address:

Larch arabinogalactan (AG), extracted from Larix gmelinii sawdust, was depolymerized by HO oxidation and purified by gel column to yield a novel degraded fraction (AGD2). The structural analysis indicated AGD2 had lower arabinose content and molecular weight compared with AG, in which the ratio of galactose and arabinose was changed from 7:3 to 16:1, the molecular weight was decreased from 50.2 kDa to 3.7 kDa, and the chain conformation spread from highly branched structure to flexible strand. It was one kind of β-D-(1 → 3)-galactan with fewer β-D-(1 → 6)-Galp side branches at O-6 position. Further, the results of the Gal-3 binding and immunomodulatory assay suggested that the unbinding force of AGD2 onto Gal-3 was as twice as AG to be 76 ± 11 pN at the loading rate of 0.15 μm/s. It could better promote the secretion of pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) than AG in a dose-dependent manner.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.01.039DOI Listing
February 2021

Lipid flip-flop and desorption from supported lipid bilayers is independent of curvature.

PLoS One 2020 30;15(12):e0244460. Epub 2020 Dec 30.

Department of Mechanical Engineering, University of Maryland, College Park, Maryland, United States of America.

Flip-flop of lipids of the lipid bilayer (LBL) constituting the plasma membrane (PM) plays a crucial role in a myriad of events ranging from cellular signaling and regulation of cell shapes to cell homeostasis, membrane asymmetry, phagocytosis, and cell apoptosis. While extensive research has been conducted to probe the lipid flip flop of planar lipid bilayers (LBLs), less is known regarding lipid flip-flop for highly curved, nanoscopic LBL systems despite the vast importance of membrane curvature in defining the morphology of cells and organelles and in maintaining a variety of cellular functions, enabling trafficking, and recruiting and localizing shape-responsive proteins. In this paper, we conduct molecular dynamics (MD) simulations to study the energetics, structure, and configuration of a lipid molecule undergoing flip-flop and desorption in a highly curved LBL, represented as a nanoparticle-supported lipid bilayer (NPSLBL) system. We compare our findings against those of a planar substrate supported lipid bilayer (PSSLBL). Our MD simulation results reveal that despite the vast differences in the curvature and other curvature-dictated properties (e.g., lipid packing fraction, difference in the number of lipids between inner and outer leaflets, etc.) between the NPSLBL and the PSSLBL, the energetics of lipid flip-flop and lipid desorption as well as the configuration of the lipid molecule undergoing lipid flip-flop are very similar for the NPSLBL and the PSSLBL. In other words, our results establish that the curvature of the LBL plays an insignificant role in lipid flip-flop and desorption.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0244460PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773258PMC
March 2021

Similar Repair Effects of Human Placenta, Bone Marrow Mesenchymal Stem Cells, and Their Exosomes for Damaged SVOG Ovarian Granulosa Cells.

Stem Cells Int 2020 3;2020:8861557. Epub 2020 Dec 3.

Reproductive Medical Center, Peking University People's Hospital, Peking University, Beijing 100044, China.

Background: This study is aimed at investigating the repairing effect of mesenchymal stem cells and their exosomes from different sources on ovarian granulosa cells damaged by chemotherapy drugs-phosphoramide mustard (PM).

Methods: In this study, we choose bone marrow mesenchymal stem cells (BMSCs) and human placental mesenchymal stem cells (HPMSCs) for research. Then, they were cocultured with human ovarian granulosa cells (SVOG) injured by phosphoramide mustard (PM), respectively. -Galactosidase staining, flow cytometry, and Western blot were used to detect the changes in the senescence and apoptosis of SVOG cells before and after their coculture with the above two types of MSCs. Subsequently, exosomes from these two types of MSCs were extracted and added to the culture medium of SVOG cells after PM injury to test whether these two types of exosomes played a role similar to that of MSCs in repairing damaged SVOG cells.

Results: PM treatment-induced apoptotic SVOG cells were significantly decreased after HPMSCs and BMSCs as compared with control group. After coculturing with these two types of MSCs, PM-treated SVOG cells showed significantly reduced senescence and apoptosis proportions as well as cleaved-Caspase 3 expression, and HPMSCs played a slightly stronger role than BMSCs in repairing SVOG cells in terms of the above three indicators. In addition, the ratios of senescent and apoptotic SVOG cells were also significantly reduced by the two types of exosomes, which played a role similar to that of MSCs in repairing cell damages.

Conclusions: The results indicated that BMSCs, HPMSCs, and their exosomes all exerted a certain repair effect on SVOG cells damaged by PM, and consistent repair effect was observed between exosomes and MSCs. The repair effect of exosomes secreted from BMSCs and HPMSCs on the SVOG cells was studied for the first time, and the results fully demonstrated that exosomes are the key carriers for MSCs to play their role.
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http://dx.doi.org/10.1155/2020/8861557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738794PMC
December 2020

Interaction between iron and dihydromyricetin extracted from vine tea.

Food Sci Nutr 2020 Nov 13;8(11):5926-5933. Epub 2020 Sep 13.

Zhejiang Academy of Forestry Hangzhou China.

In this research, the interaction between dihydromyricetin (DMY) obtained from vine tea and iron ions (Fe (II) and Fe (III)) was investigated at pH 3.0, 5.0, and 7.0 with UV absorption and fluorescence quenching spectroscopy. The effects of DMY on the stability and solubility of iron ion were also studied. The results showed the presence of iron ions changed the UV absorption spectra of DMY at the experimental pH values. And the fluorescence spectra showed that iron ion had enhanced fluorescence effect on DMY. In addition, DMY was capable of protecting Fe (II) from being oxidized and improving the solubility of Fe (III).
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http://dx.doi.org/10.1002/fsn3.1876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7684613PMC
November 2020

Structural Evolution of SiO_{2} Glass with Si Coordination Number Greater than 6.

Phys Rev Lett 2020 Nov;125(20):205701

High Pressure Collaborative Access Team, X-ray Science Division, Argonne National Laboratory, Argonne, Illinois 60439, USA.

Pair distribution function measurement of SiO_{2} glass up to 120 GPa reveals changes in the first-, second-, and third-neighbor distances associated with an increase in Si coordination number C_{Si} to >6 above 95 GPa. Packing fractions of Si and O determined from the first- and second-neighbor distances show marked changes accompanied with the structural evolution from C_{Si}=6 to >6. Structural constraints in terms of ionic radius ratio of Si and O, and ratio of nonbonded radius to bonded Si─O distance support the structural evolution of SiO_{2} glass with C_{Si}>6 at high pressures.
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http://dx.doi.org/10.1103/PhysRevLett.125.205701DOI Listing
November 2020

Micro- and Mesopores Occurring in Transitional Shales: An Examination Based on Pore Radius and Origin.

J Nanosci Nanotechnol 2021 Jan;21(1):296-309

China United Coalbed Methane Corporation Limited, Beijing, 100016, China.

The microscopic pores in shales can be characterized based on different features, including pore morphology, pore size distribution and pore origin. In this work, shale samples from the Lower Permian Shanxi Formation and Upper Carboniferous Taiyuan Formation in the Linxing Block of the Ordos Basin were examined via experiments including X-ray diffraction (XRD), total organic carbon (TOC) analysis, field emission scanning electron microscopy (FE-SEM), automatic acquisition of large-image technology (MAPS), and low-temperature gas (CO₂ and N₂) adsorption/desorption experiments. The results show that clay minerals (average value of 53.9 wt.%) and quartz (average value of 39.0 wt.%) dominate the sample composition, with some K-feldspar, plagioclase, siderite, etc. The TOC values are between 1.15% and 8.46%, and all the shales are of middle-high maturity (the vitrinite reflectance () ranges from 1.23% to 1.75%). Based on FE-SEM images, the observed pore types include interparticle (interP) pores, intraparticle (intraP) pores, and organicmatter (OM) pores, and the pores and fractures at different scales are examined by MAPS. The distributions of micro- and mesopores and the proportions of the different pore sizes were determined from the N₂ and CO₂ adsorption results. The pore size distribution results suggest that N₂ mainly penetratesmesopores (2-50 nm), while CO₂ mainly penetrates large micropores (0.7-2 nm). Micropores are the largest contributors to the total pores. The mesopores were further classified as small, medium and large mesopores, and the proportion of mesopores decreased with increasing pore size.
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http://dx.doi.org/10.1166/jnn.2021.18474DOI Listing
January 2021

Genome-wide association study reveals the genetic determinism of growth traits in a Gushi-Anka F chicken population.

Heredity (Edinb) 2021 Feb 28;126(2):293-307. Epub 2020 Sep 28.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China.

Chicken growth traits are economically important, but the relevant genetic mechanisms have not yet been elucidated. Herein, we performed a genome-wide association study to identify the variants associated with growth traits. In total, 860 chickens from a Gushi-Anka F resource population were phenotyped for 68 growth and carcass traits, and 768 samples were genotyped based on the genotyping-by-sequencing (GBS) method. Finally, 734 chickens and 321,314 SNPs remained after quality control and removal of the sex chromosomes, and these data were used to carry out a GWAS analysis. A total of 470 significant single-nucleotide polymorphisms (SNPs) for 43 of the 68 traits were detected and mapped on chromosomes (Chr) 1-6, -9, -10, -16, -18, -23, and -27. Of these, the significant SNPs in Chr1, -4, and -27 were found to be associated with more than 10 traits. Multiple traits shared significant SNPs, indicating that the same mutation in the region might have a large effect on multiple growth or carcass traits. Haplotype analysis revealed that SNPs within the candidate region of Chr1 presented a mosaic pattern. The significant SNPs and pathway enrichment analysis revealed that the MLNR, MED4, CAB39L, LDB2, and IGF2BP1 genes could be putative candidate genes for growth and carcass traits. The findings of this study improve our understanding of the genetic mechanisms regulating chicken growth and carcass traits and provide a theoretical basis for chicken breeding programs.
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http://dx.doi.org/10.1038/s41437-020-00365-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026619PMC
February 2021

Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia.

Int J Biol Macromol 2020 Dec 29;164:3305-3314. Epub 2020 Aug 29.

Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

A neutral polysaccharide (SSIPS1) was isolated and purified from cultured mycelia of Sanghuangporus sanghuang by DEAE Sepharose Fast Flow and Sephacryl S-100 columns. Basic monosaccharide composition indicated that SSIPS1 was mainly composed of d-glucose. The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6. The two branches were composed of 1,4-linked α-D-Glcp terminated with α-D-Glcp, 1,4-linked α-D-Glcp and 1,4-linked β-Galp terminated by α-D-Glcp. Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy. Further, SSIPS1 exhibited a potential inhibitory activity against α-amylase and α-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.08.202DOI Listing
December 2020
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