Publications by authors named "Yanan Zhang"

699 Publications

Indocyanine green fluorescence imaging localization-assisted thoracoscopy revision surgery after repair of esophageal atresia.

BMC Gastroenterol 2022 Aug 5;22(1):373. Epub 2022 Aug 5.

Department of Neonatal Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 100045, China.

Revision surgery for the complications after repair of esophageal atresia is often complex because of previous surgeries and chest infections and thus requires surgical expertise. This study describes surgical experiences with the use of indocyanine green (ICG) fluorescence imaging localization-assisted thoracoscopy during revision surgery, including recurrent tracheoesophageal fistula (rTEF) (8 cases, one of which was esophageal-pulmonary fistula) and delayed esophageal closure (1 case). We performed fistula repair and esophageal reconstruction according to the indications of ICG. The application of this method avoids the excessive trauma caused by freeing the trachea and esophagus. Contrast imaging taken one week and one month after surgery indicated no spillover of the contrast agent from the esophagus, except in 1 case. Indocyanine green fluorescence imaging localization-assisted thoracoscopy is worth promoting for revision surgery after esophageal atresia repair.
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http://dx.doi.org/10.1186/s12876-022-02444-1DOI Listing
August 2022

Astragaloside IV alleviates senescence of vascular smooth muscle cells through activating Parkin-mediated mitophagy.

Hum Cell 2022 Aug 4. Epub 2022 Aug 4.

Department of Cardiology, The First Affiliated Hospital of Guangdong Pharmaceutical University, No. 19, Nonglinxia Road, Yuexiu District, Guangzhou, 510080, Guangdong, China.

Astragaloside IV (AS-IV), as one of the main active components of Astragalus membranaceus, has been reported to have cardiovascular protective effects. However, the role and molecular mechanism of AS-IV in vascular senescence have not been clearly stated. The in vitro aging model was constructed using bleomycin (BLM) in vascular smooth muscle cells (VSMCs). Cell senescence were assessed through Western blotting analysis of aging markers, flow cytometry, and the β-galactosidase (SA-β-Gal) kit. Mitophagy was determined through transmission electron microscopy, TMRM staining, and Western blotting analysis of p62. A model of aging blood vessels was induced by D-gal. The vascular wall thickness of mice was also evaluated by H&E staining. Our data proved that AS-IV plays an anti-senescent role in vitro and in vivo. Results showed that AS-IV effectively improved mitochondrial injury, raised MMP, and mediated mitophagy in BLM-induced senescent VSMCs and D-gal induced aging mice. Parkin expression strengthened AS-IV's anti-senescent function. In conclusions, AS-IV attenuated BLM-induced VSMC senescence via Parkin to regulate mitophagy. Therefore, AS-IV-mediated Parkin might be a latent therapeutic agent and target for VSMC senescence.
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http://dx.doi.org/10.1007/s13577-022-00758-6DOI Listing
August 2022

Heterogeneous spectrum of CFTR gene mutations in Chinese patients with CAVD and the dilemma of genetic blocking strategy.

Reproduction 2022 Sep 14;164(3):R47-R56. Epub 2022 Jul 14.

1Department of Infertility and Sexual Medicine, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, China.

In Brief: The genetic heterogeneity of CFTR gene mutations in Chinese patients with congenital absence of the vas deferens (CAVD) differs from the hotspot mutation pattern in Caucasians. This paper reviews and suggests a more suitable screening strategy for the Chinese considering the dilemma of CFTR genetic blocking.

Abstract: Congenital absence of the vas deferens (CAVD) is a major cause of obstructive azoospermia and male infertility, with CFTR gene mutation as the main pathogenesis. Other genes such as ADGRG2, SLC9A3, and PANK2 have been discovered and proven to be associated with CAVD in recent studies. Multiple CFTR hotspot mutations have been found in Caucasians in several foreign countries, and relevant genetic counseling and preimplantation genetic diagnosis (PGD) have been conducted for decades. However, when we examined research on Chinese CAVD, we discovered that CFTR mutations show heterogeneity in the Chinese Han population, and there is currently no well-established screening strategy. Therefore, we have reviewed the literature, combining domestic and international research as well as our own, aiming to review research progress on the CFTR gene in China and discuss the appropriate scope for CFTR gene detection, the detection efficiency of other CAVD-related genes, and the screening strategy applicable to the Chinese Han population. This study provides more valuable information for genetic counseling and a theoretical basis for PGD and treatment for couples with CAVD when seeking reproductive assistance.
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http://dx.doi.org/10.1530/REP-21-0315DOI Listing
September 2022

Analysis of the Taxonomy, Synteny, and Virulence Factors for Soft Rot Pathogen in Using Comparative Genomics.

Front Microbiol 2022 13;13:868709. Epub 2022 Jul 13.

College of Life Sciences, Jiangxi Normal University, Nanchang, China.

Bacterial soft rot is a devastating disease for a wide range of crops, vegetables, and ornamental plants including konjac (). However, the pangenome and genomic plasticity of the konjac soft rot pathogens is little explored. In this study, we reported the complete genome sequences of 11 bacterial isolates that can cause typical soft rot symptoms in konjac by and pathogenicity tests. Based on DNA-DNA hybridization, average nucleotide identity and phylogenomic analysis, all 11 isolates were determined to be . In addition, synteny analysis of these genomes revealed considerable chromosomal inversions, one of which is triggered by homologous recombination of ribose operon. Pangenome analysis and COG enrichment analysis showed that the pangenome of . is open and that accessory genes are enriched in replication, recombination, and repair. Variations in type IV secretion system and type VI secretion system were found, while plant cell wall degrading enzymes were conserved. Furthermore, sequence analyses also provided evidence for the presence of a type V secretion system in . These findings advance our understanding of the pathogenicity determinants, genomic plasticity, and evolution of . .
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http://dx.doi.org/10.3389/fmicb.2022.868709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326479PMC
July 2022

Phenotypic Changes of Peripheral γδ T Cell and Its Subsets in Patients With Coronary Artery Disease.

Front Immunol 2022 8;13:900334. Epub 2022 Jul 8.

National Center for International Research of Bio-targeting Theranostics, Guangxi Key Laboratory of Bio-targeting Theranostics, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Talent Highland of Bio-targeting Theranostics, Guangxi Medical University, Nanning, China.

Coronary atherosclerotic heart disease (CAD) is a chronic inflammatory cardiovascular disease with high morbidity and mortality. Growing data indicate that many immune cells are involved in the development of atherosclerosis. However, the immunological roles of γδ T cells in the initiation and progression of CAD are not fully understood. Here, we used flow cytometry to determine phenotypical changes of γδ T cells and their subpopulations in peripheral blood samples collected from 37 CAD patients. The Pearson correlation coefficient was used to analyze the relationship between the clinical parameter (serum LDL-C level) and the changes of immunophenotypes of γδ T cells. Our results demonstrated that the frequencies and absolute numbers of total γδ T cells and Vδ2 T cells were significantly decreased in CAD patients when compared to healthy individuals. However, the proportion of Vδ1 T cells was much lower in CAD patients than that of healthy individuals. Most importantly, a significant alteration of the Vδ1/Vδ2 ratio was found in CAD patients. In addition, a series of surface markers that are associated with costimulatory signals (CD28, CD40L, CD80, CD86), activation levels (CD69, CD25, HLA-DR), activating NK cell receptors (NKp30, NKp46, NKG2D) and inhibitory receptors (PD-1, CTLA-4, PD-1, Tim-3) were determined and then analyzed in the total γδ T cells, Vδ2T cells and Vδ2T cells of CAD patients and healthy individuals. The data demonstrated that immunological activities of total γδ T cells, Vδ2T cells, and Vδ2T cells of CAD patients were much lower than those in healthy individuals. Moreover, we found that there were positive correlations between the serum LDL-C levels and frequencies of CD3γδ T cells, CD69Vδ2T cells, NKG2DVδ2T cells, and NKp46Vδ2T cells. By contrast, there was an inverse correlation between the levels of serum LDL-C and the frequencies of CD69Vδ2T cells and NKp46Vδ2T cells. Accordingly, these findings could help us to better understand the roles of γδ T cells in the CAD, and shed light on the development of novel diagnostic techniques and therapeutic strategies by targeting γδ T cells for CAD patients.
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http://dx.doi.org/10.3389/fimmu.2022.900334DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304556PMC
July 2022

Pre-Clinical Development of a Potent Neutralizing Antibody MW3321 With Extensive SARS-CoV-2 Variants Coverage.

Front Pharmacol 2022 8;13:926750. Epub 2022 Jul 8.

Mabwell (Shanghai) Bioscience Co., Ltd., Shanghai, China.

Since the outbreak of the coronavirus disease 2019 (COVID-19) pandemic, several variants of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have emerged and have consistently replaced the previous dominant variant. Therapeutics against variants of SARS-CoV-2 are urgently needed. Ideal SARS-CoV-2 therapeutic antibodies would have high potency in viral neutralization against several emerging variants. Neutralization antibodies targeting SARS-CoV-2 could provide immediate protection after SARS-CoV-2 infection, especially for the most vulnerable populations. In this work, we comprehensively characterize the breadth and efficacy of SARS-CoV-2 RBD-targeting fully human monoclonal antibody (mAb) MW3321. MW3321 retains full neutralization activity to all tested 12 variants that have arisen in the human population, which are assigned as VOC (Variants of Concern) and VOI (Variants of Interest) due to their impacts on public health. Escape mutation experiments using replicating SARS-CoV-2 pseudovirus show that escape mutants were not generated until passage 6 for MW3321, which is much more resistant to escape mutation compared with another clinical staged SARS-CoV-2 neutralizing mAb MW3311. MW3321 could effectively reduce viral burden in hACE2-transgenic mice challenged with either wild-type or Delta SARS-CoV-2 strains through viral neutralization and Fc-mediated effector functions. Moreover, MW3321 exhibits a typical hIgG1 pharmacokinetic and safety profile in cynomolgus monkeys. These data support the development of MW3321 as a monotherapy or cocktail against SARS-CoV-2-related diseases.
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http://dx.doi.org/10.3389/fphar.2022.926750DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304585PMC
July 2022

Growth Differentiation Factor 15 Regulates Oxidative Stress-Dependent Ferroptosis Post Spinal Cord Injury by Stabilizing the p62-Keap1-Nrf2 Signaling Pathway.

Front Aging Neurosci 2022 4;14:905115. Epub 2022 Jul 4.

Department of Orthopedics, Taizhou People's Hospital, Nanjing Medical University, Taizhou, China.

Background: Spinal cord injury (SCI) is a severe traumatic disorder of the central nervous system (CNS) that causes irreversible damage to the nervous tissue. The consequent hemorrhage contributed by trauma induces neuronal ferroptosis post SCI, which is an important death mode to mediate neuronal loss. Growth differentiation factor 15 (GDF15) is a cytokine that regulates cell proliferation, differentiation, and death. However, the specific role of GDF15 in neuronal ferroptosis post SCI remains unknown.

Materials And Methods: Neuronal ferroptosis was measured by detection of lipid peroxidation, glutathione, iron content, and reactive oxidative stress. , western blotting and immunofluorescence (IF) staining was utilized to measure ferroptosis post SCI. IF staining, TUNEL staining, hematoxylin-eosin staining, and Nissl staining were used to measure neurological damage. Finally, locomotor function recovery was analyzed using the Basso Mouse Scale and Louisville Swim Scale.

Results: GDF15 was significantly increased in neuronal ferroptosis and silencing GDF15 aggravated ferroptosis both and . Besides, GDF15-mediated inhibition of neuronal ferroptosis is through p62-dependent Keap1-Nrf2 pathway. In SCI mice, knockdown of GDF15 significantly exacerbated neuronal death, interfered with axon regeneration and remyelination, aggravated ferroptosis-mediated neuroinflammation, and restrained locomotor recovery.

Conclusion: GDF15 effectively alleviated neuronal ferroptosis post SCI the p62-Keap1-Nrf2 signaling pathway and promoted locomotor recovery of SCI mice, which is suggested as a potential target on SCI pathogenesis and treatment.
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http://dx.doi.org/10.3389/fnagi.2022.905115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289442PMC
July 2022

Chromosome-level assembly and analysis of the Thymus genome provide insights on glandular secretory trichome formation and monoterpenoid biosynthesis in thyme.

Plant Commun 2022 Jul 15:100413. Epub 2022 Jul 15.

Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China. Electronic address:

Thyme has medicinal and aromatic value because of its potent antimicrobial and antioxidant properties. However, the absence of a fully sequenced thyme genome limits functional genomic studies in Chinese native thymes. Thymus quinquecostatus Celak., which contains large amounts of bioactive monoterpenes, such as thymol and carvacrol, is an important wild medicinal and aromatic plant in China. Monoterpenoids are abundant in glandular secretory trichomes. Here, high-fidelity and chromatin conformation capture technologies were used to assemble and annotate the T. quinquecostatus genome at the chromosome level. The 13 chromosomes of T. quinquecostatus had a total length of 528.66 megabases (Mb), a contig N50 of 8.06 Mb, and BUSCO of 97.34%. We found that T. quinquecostatus experienced two whole-genome duplications, with the most recent event occurring ∼4.34 million years ago. Deep analyses of the genome, in conjunction with comparative genomic, phylogenetic, transcriptomic, and metabonomic studies, uncovered many regulatory factors and genes related to monoterpenoids and glandular secretory trichome development. Terpene synthase (TPS)-, cytochrome P450 monooxygenases (CYP)-, short-chain dehydrogenase/reductase (SDR)-, R2R3-MYB-, and homeodomain-leucine zipper (HD-ZIP) IV-encoding genes were among those present in the T. quinquecostatus genome. Notably, Tq02G002290.1 (TqTPS1) was shown to be the gene encoding the terpene synthase responsible for catalyzing geranyl diphosphate (GPP) to produce the main monoterpene product γ-terpinene. Our study provides significant insight into the mechanism of glandular secretory trichome formation and monoterpenoid biosynthesis in thyme. Furthermore, this work will facilitate the development of molecular breeding tools to enhance the productivity of bioactive secondary metabolites in Lamiaceae.
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http://dx.doi.org/10.1016/j.xplc.2022.100413DOI Listing
July 2022

Applications of polymerase chain reaction-based methods for the diagnosis of plague (Review).

Exp Ther Med 2022 Aug 14;24(2):511. Epub 2022 Jun 14.

Inner Mongolia Key Laboratory of Disease-Related Biomarkers, Baotou Medical College, Baotou, Inner Mongolia 014060, P.R. China.

Plague is an acute bacterial infection caused by . The three major clinical forms of plague are bubonic, pneumonic and septicemic, which have high case-mortality rates. Therefore, rapid and reliable diagnostic tools are crucial. Currently, bacteriological means and traditional serological assays are used for detecting infection with . However, such methods have their limitations. Polymerase chain reaction (PCR) is one of the most useful tools for rapid diagnosis of plague. The present review introduced the main PCR techniques and their applications for detecting and confirmation of . The advantages and disadvantages of the different PCR methods were also summarized.
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http://dx.doi.org/10.3892/etm.2022.11438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257939PMC
August 2022

Isolation and Genome Sequence of a Novel Phosphate-Solubilizing Rhizobacterium Bacillus altitudinis GQYP101 and Its Effects on Rhizosphere Microbial Community Structure and Functional Traits of Corn Seedling.

Curr Microbiol 2022 Jul 14;79(9):249. Epub 2022 Jul 14.

College of Life Sciences and Shandong Engineering Research Center of Plant, Microbia Restoration for Saline-Alkali Land and Shandong Key Laboratory of Agricultural Microbiology and National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, Taian, China.

Bacillus altitudinis is a widely distributed soil bacterium that has various functional activities, including remediation of contaminated soil, degradation of herbicides, and enhancement of plant growth. B. altitudinis GQYP101 was isolated from the rhizosphere soil of Lycium barbarum L. and demonstrated potential as a plant growth-promoting bacterium. In this work, strain GQYP101 could solubilize phosphorus, and increased the stem diameter, maximum leaf area, and fresh weight of corn in a pot experiment. Nitrogen and phosphorus contents of corn seedlings (aerial part) increased by 100% and 47.9%, respectively, after application of strain GQYP101. Concurrently, nitrogen and phosphorus contents of corn root also increased, by 55.40% and 20.3%, respectively. Furthermore, rhizosphere soil nutrients were altered and the content of available phosphorus increased by 73.2% after application of strain GQYP101. The mechanism by which strain GQYP101 improved plant growth was further investigated by whole genome sequence analysis. Strain GQYP101 comprises a circular chromosome and a linear plasmid. Some key genes of strain GQYP101 were identified that were related to phosphate solubilization, alkaline phosphatase, chemotaxis, and motility. The findings of this study may provide a theoretical basis for strain GQYP101 to enhance crop yield as microbial fertilizer.
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http://dx.doi.org/10.1007/s00284-022-02944-zDOI Listing
July 2022

DDQN with Prioritized Experience Replay-Based Optimized Geographical Routing Protocol of Considering Link Stability and Energy Prediction for UANET.

Sensors (Basel) 2022 Jul 3;22(13). Epub 2022 Jul 3.

School of Information and Communications, Guilin University of Electronic Technology, Guilin 541004, China.

Unmanned aerial vehicles (UAVs) are important equipment for efficiently executing search and rescue missions in disaster or air-crash scenarios. Each node can communicate with the others by a routing protocol in UAV ad hoc networks (UANETs). However, UAV routing protocols are faced with the challenges of high mobility and limited node energy, which hugely lead to unstable link and sparse network topology due to premature node death. Eventually, this severely affects network performance. In order to solve these problems, we proposed the deep-reinforcement-learning-based geographical routing protocol of considering link stability and energy prediction (DSEGR) for UANETs. First of all, we came up with the link stability evaluation indicator and utilized the autoregressive integrated moving average (ARIMA) model to predict the residual energy of neighbor nodes. Then, the packet forward process was modeled as a Markov Decision Process, and according to a deep double Q network with prioritized experience replay to learn the routing-decision process. Meanwhile, a reward function was designed to obtain a better convergence rate, and the analytic hierarchy process (AHP) was used to analyze the weights of the considered factors in the reward function. Finally, to verify the effectiveness of DSEGR, we conducted simulation experiments to analyze network performance. The simulation results demonstrate that our proposed routing protocol remarkably outperforms others in packet delivery ratio and has a faster convergence rate.
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http://dx.doi.org/10.3390/s22135020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269789PMC
July 2022

Recent Advances in Ionic Liquids and Ionic Liquid-Functionalized Graphene: Catalytic Application and Environmental Remediation.

Int J Environ Res Public Health 2022 Jun 21;19(13). Epub 2022 Jun 21.

Heng Sheng Water Environment Treatment Co., Ltd., Guilin 541100, China.

Applications of ionic liquids (ILs) for the modification physicochemical properties of porous materials have been extensively studied with respect to various applications based on the understanding and development of properties of ILs. In this review, IL-graphene composites are discussed and provided a perspective of composites of IL. IL has been used as a medium to improve the dispersibility of graphene, and the resulting composite material shows excellent performance in gas separation and catalysis during environmental treatment. The applications of ILs and IL-functionalized graphene are discussed in detail with the actual environmental issues, and the main challenges and opportunities for possible future applications are summarized.
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http://dx.doi.org/10.3390/ijerph19137584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325325PMC
June 2022

Establishment of a predictive model for purulent meningitis in preterm infants.

Transl Pediatr 2022 Jun;11(6):1018-1027

Department of Neonatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: Purulent meningitis (PM) is an important cause of mortality and morbidity in the newborn population throughout the world. The subtle of specific clinical signs and low success rates of lumbar puncture make diagnosis of PM more difficult in preterm than in older children. The objective of this study was to establish a predict model for preterm PM in hopes of helping clinicians develop new diagnostic and treatment strategies.

Methods: Premature infants who were admitted to The First Affiliated Hospital of Zhengzhou University from September 2017 to March 2020 were enrolled in this study. All the patients underwent lumbar puncture. We collected data encompassing maternal diseases and neonatal clinical features. Cerebrospinal fluid (CSF) culture is the gold standard for diagnosing meningitis. The PM was diagnosed according to the diagnostic criteria. All statistical analyses were performed using R 3.63 (https://www.r-project.org/). Logistic regression and least absolute shrinkage and selection operator (LASSO) regression analyses were used to establish a risk prediction model of PM. The Brier score, calibration slope, and concordance (C)-index were used to verify the accuracy of prediction model.

Results: A total of 168 preterm infants were enrolled in this study, 80 boys and 88 girls, the gestational age (GA) was 26.43-36.86 weeks (32.45±2.79 weeks), the birth weight (BW) was 700-3,400 g (1,814.05±568.84 g). There were 77 preterm infants with PM while 91 without. We identified seven variables as independent risk factors for PM in preterm infants by LASSO analysis [the optimal λ was 0.080960, and log(λ) = -2.5138], including procalcitonin (PCT) on the 1st day after birth, prenatal glucocorticoid use, albumin, the 1-minute Apgar score, the use of non-invasive biphasic positive airway pressure, hemoglobin, and sex. These were used to construct a risk prediction nomogram and verified its accuracy. The Brier score was 0.17, the calibration slope was 0.966, and the concordance index was 0.82018.

Conclusions: Our prediction model could predict the risk of PM in preterm infants. Using this prediction model, it may be able to provide reference to determine whether lumbar puncture is performed and whether antibiotics are applied as soon as possible.
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http://dx.doi.org/10.21037/tp-22-236DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253939PMC
June 2022

Comprehensive Analysis of Gut Microbiota and Fecal Bile Acid Profiles in Children With Biliary Atresia.

Front Cell Infect Microbiol 2022 17;12:914247. Epub 2022 Jun 17.

Department of Neonatal Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.

Background: Biliary atresia (BA) is the most common cholestatic liver disease in neonates. Herein, we aimed at characterizing the gut microbiota and fecal bile acid profiles of BA patients, defining the correlations between them, and evaluating the relationship between the clinical pathogenesis and changes in the gut microbiota and bile acid profiles.

Methods: A total of 84 fecal samples from BA patients (n = 46) and matched healthy controls (HCs, n = 38) were subjected to sequencing by 16S rRNA gene amplification, and fecal bile acid were analyzed by targeted metabolomics.

Findings: Compared with the controls, a structural separation of the intestinal flora of BA patients was uncovered, which was accompanied by changes in the composition of fecal bile acids. In the BA group, , , and were enriched in patients without cholangitis ( < 0.05). and were more abundant in patients without recurrent cholangitis episodes ( < 0.05), while and were enriched in patients with multiple recurrences of cholangitis ( < 0.05). Postoperative jaundice clearance was associated with and ( < 0.05), and tauroursodeoxycholic acid was associated with jaundice clearance ( < 0.001).

Conclusion: BA patients are characterized by different compositions of gut microbiota and bile acids, and their interaction is involved in the process of liver damage in BA, which may be closely related to the occurrence of postoperative cholangitis and jaundice clearance.
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http://dx.doi.org/10.3389/fcimb.2022.914247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247268PMC
July 2022

Willingness to Pay for Green Office: Evidence from Shanghai.

Environ Sci Pollut Res Int 2022 Jul 2. Epub 2022 Jul 2.

Antai College of Economics and Management, Shanghai Jiao Tong University, Shanghai 200030, China; Institute of Eco-Chongming, 20 Cuiniao Rd.Chenjia Zhen, Chongming, Shanghai, 202162, China.

The construction sector is a critical contributor to energy consumption and greenhouse gas emission. Promotion of green buildings may be a good way to tackle environmental problems. In this study, a choice experiment was undertaken to explore preferences and willingness to pay (WTP) for various attributes of green office buildings in Shanghai, with a sample of 309 respondents. Results of the mixed logit reveal that respondents value attributes relating to indoor comfortability and health, including Quiet Office Environment, Eco-Friendly Construction and Decoration Material, and Indoor Air Purification. Higher WTP is also observed for features of Power Saving and Water Saving, which provide both private and public benefits. According to heterogeneity analysis, people working in offices with a higher rent are more likely to pay for air purifier installation. In general, construction & manufacturing firms have higher WTP for Power Saving while lower WTP for Soundproof. On average, foreign firms are willing to pay more for eco-friendly materials. Finally, implications and suggestions for policymakers and building developers are discussed.
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http://dx.doi.org/10.1007/s11356-022-21555-6DOI Listing
July 2022

Factors Affecting the Postoperative Bowel Function and Recurrence of Surgery for Intestinal Deep Endometriosis.

Front Surg 2022 14;9:914661. Epub 2022 Jun 14.

Department of General Gynecology, Women's Hospital School of Medicine Zhejiang University, Hangzhou, China.

Objective: This study aims to evaluate the factors associated with complications and long-term results in the surgical treatment of intestinal deep endometriosis and to figure out the optimized treatment measures for bowel endometriosis.

Methods: A retrospective study was performed in a single center in China. Medical charts were reviewed from 61 women undergoing surgical treatment for bowel endometriosis between January 2013 and August 2019 in the Department of General Gynecology, Women's Hospital School of Medicine Zhejiang University. Multivariate regression analysis was utilized to investigate the impact of the stages of endometriosis and surgical steps (independent risk factors) on complications (and postoperative bowel dysfunction). The clinical characters, surgical procedures, postoperative treatment, complications, and recurrence rate were summarized and analyzed by using Lasso regression.

Results: Surgery type was the most important independent risk factor related to postoperative abnormal defecation in intestinal deep endometriosis patients ( < 0.05, OR = 34.133). Infection is the most important independent risk factor related to both postoperative complications (OR = 96.931) and recurrences after conservative surgery (OR = 4.667). Surgery type and age were significantly related to recurrences after conservative surgery.

Conclusions: We recommended conservative operation especially full-thickness disc excision to improve the quality of life of intestinal deep endometriosis patients. In addition, prevention of infection is very important to reduce the postoperative complications rate and the recurrence rate.
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http://dx.doi.org/10.3389/fsurg.2022.914661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239406PMC
June 2022

First report of Pseudomonas palleroniana causing potato soft rot in China.

Plant Dis 2022 Jun 30. Epub 2022 Jun 30.

Qujing Normal University, 105872, Qujing, Yunnan, China;

Slimy potato (Solanum tuberosum) tubers with a foul-smelling odor were obtained from a potato field during harvest in Huize county (25.94°N; 103.40°E), Yunnan province, China in August 2021. The incidence of symptomatic potato tubers was approximately 5% while no symptoms were observed on potato stems. To isolate the causal agent, potato tubers were surface-disinfected and infected tissues were mashed in a 15 ml sterile centrifuge tube with 2 ml sterile distilled water. After a series of dilutions, a volume of 200 μl bacterial suspension from each dilution was spread on nutrient agar (NA) medium and incubated at 28 °C for 48 h. Single colonies randomly selected from NA plates were then picked and separately subcultured in nutrient broth (NB) medium. Pure cultures were acquired by successive streaking on NA medium. The colonies with irregular shapes were white and opaque (Fig. S1A). Among all the isolates, strain Q1 exhibited pectinolytic activity on inoculated potato tubers using a pin prick method with an inoculum concentration of 108 CFU/ml while no symptoms appeared for the control group using sterile water (Fig. S1B). However, no obvious pits were observed for strain Q1 when tested on crystal violet pectin (CVP) medium. To further identify the strain Q1, total DNA was extracted using the TaKaRa MiniBEST Bacteria Genomic DNA Extraction Kit. PCR amplification of 16S rDNA of strain Q1 was performed using the universal PCR primer pair 27F/1492R and followed by Sanger sequencing. The BLASTn analysis of the sequence (NCBI accession number ON631256) based on NCBI rRNA/ITS databases revealed 100% query coverage and 99.78% identity to the 16S rDNA sequences of type strain Pseudomonas tolaasii ATCC 33618 and Pseudomonas palleroniana CFBP 4389, respectively. Whole-genome sequencing of strain Q1 was then performed using the Illumina and Nanopore sequencing platform. A single contig (NCBI accession number CP092411) with a length of approx. 6.2 MB was obtained by de novo assembly using Raven (v1.5.1) (Vaser and Šikić 2021) and Pilon (v1.24) (Walker et al. 2014). The completeness and redundancy of this assembly was evaluated by BUSCO (v5.2.2) (Manni et al. 2021), and a score of 100% completeness indicated a high quality of the assembly. The genome sequence of strain Q1 was uploaded to the Type Strain Genome Server (TYGS) for a whole genome-based taxonomic analysis (Meier-Kolthoff and Göker 2019). The distance-based phylogeny revealed that strain Q1 is well clustered together with Pseudomonas palleroniana (Fig. S1C). Furthermore, a digital DNA-DNA hybridization (dDDH) value (86.9%) between strain Q1 and P. palleroniana was above the species boundary (≥70%), indicating that strain Q1 should be classified as P. palleroniana. In addition, P. palleroniana was re-isolated from potato tubers inoculated using a pin prick method with an inoculum concentration of 108 CFU/ml and was identified by morphological similarities and 16s rDNA sequencing described above, thus fulfilling Koch's postulates. Furthermore, aerial stem rot symptoms were observed after 3-5 days post inoculation of strain Q1 with a concentration of 108 CFU/ml on stems of young potato plants using a pin prick method (Fig. S1D). Notably, a couple of Pseudomonas spp. have been reported to cause soft rot in carrot (Godfrey and Marshall 2002) and melon (Zhang et al. 2016). To our knowledge, this is the first report of P. palleroniana causing soft rot on potato tuber in China.
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http://dx.doi.org/10.1094/PDIS-04-22-0816-PDNDOI Listing
June 2022

Detection of Stable Elite Haplotypes and Potential Candidate Genes of Boll Weight Across Multiple Environments via GWAS in Upland Cotton.

Front Plant Sci 2022 13;13:929168. Epub 2022 Jun 13.

College of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, China.

Boll weight (BW) is a key determinant of yield component traits in cotton, and understanding the genetic mechanism of BW could contribute to the progress of cotton fiber yield. Although many yield-related quantitative trait loci (QTLs) responsible for BW have been determined, knowledge of the genes controlling cotton yield remains limited. Here, association mapping based on 25,169 single-nucleotide polymorphisms (SNPs) and 2,315 insertions/deletions (InDels) was conducted to identify high-quality QTLs responsible for BW in a global collection of 290 diverse accessions, and BW was measured in nine different environments. A total of 19 significant markers were detected, and 225 candidate genes within a 400 kb region (± 200 kb surrounding each locus) were predicted. Of them, two major QTLs with highly phenotypic variation explanation on chromosomes A08 and D13 were identified among multiple environments. Furthermore, we found that two novel candidate genes ( and ) were associated with BW and that was involved in artificial selection during cotton breeding by population genetic analysis. The transcription level analyses showed that these two genes were significantly differentially expressed between high-BW accession and low-BW accession during the ovule development stage. Thus, these results reveal valuable information for clarifying the genetic basics of the control of BW, which are useful for increasing yield by molecular marker-assisted selection (MAS) breeding in cotton.
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http://dx.doi.org/10.3389/fpls.2022.929168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234699PMC
June 2022

Dynamic Quantitative Trait Loci Mapping for Plant Height in Recombinant Inbred Line Population of Upland Cotton.

Front Plant Sci 2022 9;13:914140. Epub 2022 Jun 9.

State Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Taian, China.

Plant height (PH) is a key plant architecture trait for improving the biological productivity of cotton. Ideal PH of cotton is conducive to lodging resistance and mechanized harvesting. To detect quantitative trait loci (QTL) and candidate genes of PH in cotton, a genetic map was constructed with a recombinant inbred line (RIL) population of upland cotton. PH phenotype data under nine environments and three best linear unbiased predictions (BLUPs) were used for QTL analyses. Based on restriction-site-associated DNA sequence (RAD-seq), the genetic map contained 5,850 single-nucleotide polymorphism (SNP) markers, covering 2,747.12 cM with an average genetic distance of 0.47 cM. Thirty-seven unconditional QTL explaining 1.03-12.50% of phenotypic variance, including four major QTL and seven stable QTL, were identified. Twenty-eight conditional QTL explaining 3.27-28.87% of phenotypic variance, including 1 major QTL, were identified. Importantly, five QTL, including 4 stable QTL, were both unconditional and conditional QTL. Among the 60 PH QTL (including 39 newly identified), none of them were involved in the whole period of PH growth, indicating that QTL related to cotton PH development have dynamic expression characteristics. Based on the functional annotation of homologous genes and transcriptome data of upland cotton TM-1, 14 candidate genes were predicted within 10 QTL. Our research provides valuable information for understanding the genetic mechanism of PH development, which also increases the economic production of cotton.
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http://dx.doi.org/10.3389/fpls.2022.914140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235862PMC
June 2022

Overexpression of GhABF3 increases cotton(Gossypium hirsutum L.) tolerance to salt and drought.

BMC Plant Biol 2022 Jun 29;22(1):313. Epub 2022 Jun 29.

State Key Laboratory of Crop Biology/Agronomy College, Shandong Agricultural University, Taian, China.

Background: Plants suffer from various abiotic stresses during their lifetime, of which drought and salt stresses are two main factors limiting crop yield and quality. Previous studies have shown that abscisic acid (ABA) responsive element binding protein (AREB)/ ABRE binding factors (ABFs) in bZIP transcription factors are involved in plant stress response in an ABA-dependent manner. However, little is known about the properties and functions of AREB/ABFs, especially ABF3, in cotton.

Results: Here, we reported the cloning and characterization of GhABF3. Expression of GhABF3 was induced by drought,salt and ABA treatments. Silencing of GhABF3 sensitized cotton to drought and salt stress, which was manifested in decreased cellular antioxidant capacity and chlorophyll content. Overexpression of GhABF3 significantly improved the drought and salinity tolerance of Arabidopsis and cotton. Exogenous expression of GhABF3 resulted in longer root length and less leaf wilting under stress conditions in Arabidopsis thaliana. Overexpressing GhABF3 significantly improved salt tolerance of upland cotton by reducing the degree of cellular oxidation, and enhanced drought tolerance by decreasing leaf water loss rate. The increased expression of GhABF3 up-regulated the transcriptional abundance of downstream ABA-inducible genes under salt stress in Arabidopsis.

Conclusion: In conclusion, our results demonstrated that GhABF3 plays an important role in plant drought and salt tolerance. Manipulation of GhABF3 by biotechnology might be an important strategy to alter the stress resistance of cotton.
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http://dx.doi.org/10.1186/s12870-022-03705-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241229PMC
June 2022

Molecular Mechanism of β-Sitosterol and its Derivatives in Tumor Progression.

Front Oncol 2022 8;12:926975. Epub 2022 Jun 8.

Department of Pathology, Shandong University of Traditional Chinese Medicine, Jinan, China.

β-Sitosterol (SIT), a white powdery organic substance with a molecular formula of CHO, is one of the most abundant naturally occurring phytosterols in plants. With a chemical composition similar to that of cholesterol, SIT is applied in various fields such as medicine, agriculture, and chemical industries, owing to its unique biological and physicochemical properties. Modern pharmacological studies have elucidated good anti-tumor therapeutic effect activity of SIT, which mainly manifests as pro-apoptotic, anti-proliferative, anti-metastatic, anti-invasive, and chemosensitizing on tumor cells. In addition, SIT exerts an anti-tumor effect on multiple malignant tumors such as breast, gastric, lung, kidney, pancreatic, prostate, and other cancers. Further, SIT derivatives with structural modifications are promising anti-tumor drugs with significant anti-tumor effects. This review article focuses on recent studies relevant to the anti-tumor effects of SIT and summarizes its anti-tumor mechanism to provide a reference for the clinical treatment of malignant tumors and the development of novel anti-tumor drugs.
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http://dx.doi.org/10.3389/fonc.2022.926975DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213880PMC
June 2022

Probabilistic Statistics-Based Endurance Life Prediction of Bridge Structures.

Authors:
Yanan Zhang

Comput Intell Neurosci 2022 15;2022:8035028. Epub 2022 Jun 15.

School of Mathematics and Computer Science, Shaanxi University of Technology, Hanzhong 723001, China.

With the massive construction of bridge infrastructures, bridge health monitoring systems have gradually matured in application and research, but previous research has primarily focused on structural damage detection and bridge safety warnings based on valid data. The structural details of steel bridge panels and structural systems are determined by the coupling effects of many intrinsic and extrinsic uncertainties, such as material properties, structural characteristics, manufacturing processes, and random traffic loads. The evaluation of fatigue is a difficult task. This article first builds a big data platform, utilizing its high-efficiency parallel computing capability and highly fault-tolerant distributed file system to achieve second-level monitoring data processing; ensuring real-time data cleaning, data analysis, and safety warning; and building a big data analysis and processing platform with high reliability, high availability, high storage efficiency, and high scalability of bridge health monitoring. The big data platform chooses HDFS for offline data storage and Spark for data analysis and modelling after comparing and analysing the benefits and drawbacks of various big data technologies. Kafka is used for caching real-time data, and Spark-streaming is used for reading data and real-time processing. Finally, the platform's superiority and reliability in terms of offline computing performance, real-time online performance, scalability, and fault tolerance are confirmed through experimental analysis; the optimal data cleaning method is derived by comparing and analysing monitoring data noise, jump point, and drift phenomena. This part of the research is based on bridge temperature data with stable signals and bridge strain data with fluctuating signals, taking into account the influence of different data types; the corresponding data missing repair algorithms are proposed for different types of data to form a complete and general data patching method process. The probabilistic fracture mechanics theory, in comparison to the traditional deterministic fatigue assessment method, can better reflect the essential uncertainty of fatigue problems and is an effective way to assess the fatigue performance of orthotropic steel bridge decks. The goal of data patching is to ensure data recovery accuracy of over 90%, with no patching repair required for monitoring data with too much missing data. The endurance life of bridge structures is predicted using a big data probabilistic statistics approach based on a variety of factors such as material properties, construction characteristics, manufacturing processes, and random traffic loads.
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http://dx.doi.org/10.1155/2022/8035028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9217558PMC
June 2022

Lignin Biosynthesis Pathway and Redox Balance Act Synergistically in Conferring Resistance against Infection in 7-Demethoxytylophorine-Treated Navel Orange.

J Agric Food Chem 2022 Jul 22;70(26):8111-8123. Epub 2022 Jun 22.

Department of Horticulture, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China.

7-Demethoxytylophorine (DEM), a natural water-soluble phenanthroindolizidine alkaloid, has a great potential for suppression of growth. In the present study, we investigated the ability of DEM to confer resistance against in harvested "Newhall" navel orange and the underlying mechanism. Results from the experiment showed that DEM treatment delayed blue mold development. The water-soaked lesion diameter in 40 mg L DEM-treated fruit was 35.2% lower than that in the control after 96 h. Moreover, the decrease in peel firmness loss and increase in electrolyte leakage, superoxide anion (O) production, and malondialdehyde (MDA) content were significantly inhibited by DEM treatment. Hydrogen peroxide (HO) burst in DEM-treated fruit at the early stage of infection contributed to the conferred resistance by increasing the activities of lignin biosynthesis-related enzymes, along with the expressions of their encoding genes, resulting in lignin accumulation. The DEM-treated fruit maintained an elevated antioxidant capacity, as evidenced by high levels of ascorbic acid and glutathione content, and enhanced or upregulated the activities and gene expression levels of APX, GR, MDHAR, DHAR, GPX, and GST, thereby maintaining ROS homeostasis and reducing postharvest blue mold. Collectively, the results in the present study revealed a control mechanism in which DEM treatment conferred the resistance against infection in harvested "Newhall" navel orange fruit by activating lignin biosynthesis and maintaining the redox balance.
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http://dx.doi.org/10.1021/acs.jafc.2c02348DOI Listing
July 2022

Pinpointing novel risk loci for Lewy body dementia and the shared genetic etiology with Alzheimer's disease and Parkinson's disease: a large-scale multi-trait association analysis.

BMC Med 2022 06 22;20(1):214. Epub 2022 Jun 22.

Department of Biostatistics, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, 250012, Shandong, China.

Background: The current genome-wide association study (GWAS) of Lewy body dementia (LBD) suffers from low power due to a limited sample size. In addition, the genetic determinants underlying LBD and the shared genetic etiology with Alzheimer's disease (AD) and Parkinson's disease (PD) remain poorly understood.

Methods: Using the largest GWAS summary statistics of LBD to date (2591 cases and 4027 controls), late-onset AD (86,531 cases and 676,386 controls), and PD (33,674 cases and 449,056 controls), we comprehensively investigated the genetic basis of LBD and shared genetic etiology among LBD, AD, and PD. We first conducted genetic correlation analysis using linkage disequilibrium score regression (LDSC), followed by multi-trait analysis of GWAS (MTAG) and association analysis based on SubSETs (ASSET) to identify the trait-specific SNPs. We then performed SNP-level functional annotation to identify significant genomic risk loci paired with Bayesian fine-mapping and colocalization analysis to identify potential causal variants. Parallel gene-level analysis including GCTA-fastBAT and transcriptome-wide association analysis (TWAS) was implemented to explore novel LBD-associated genes, followed by pathway enrichment analysis to understand underlying biological mechanisms.

Results: Pairwise LDSC analysis found positive genome-wide genetic correlations between LBD and AD (rg = 0.6603, se = 0.2001; P = 0.0010), between LBD and PD (rg = 0.6352, se = 0.1880; P = 0.0007), and between AD and PD (rg = 0.2136, se = 0.0860; P = 0.0130). We identified 13 significant loci for LBD, including 5 previously reported loci (1q22, 2q14.3, 4p16.3, 4q22.1, and 19q13.32) and 8 novel biologically plausible genetic associations (5q12.1, 5q33.3, 6p21.1, 8p23.1, 8p21.1, 16p11.2, 17p12, and 17q21.31), among which APOC1 (19q13.32), SNCA (4q22.1), TMEM175 (4p16.3), CLU (8p21.1), MAPT (17q21.31), and FBXL19 (16p11.2) were also validated by gene-level analysis. Pathway enrichment analysis of 40 common genes identified by GCTA-fastBAT and TWAS implicated significant role of neurofibrillary tangle assembly (GO:1902988, adjusted P = 1.55 × 10).

Conclusions: Our findings provide novel insights into the genetic determinants of LBD and the shared genetic etiology and biological mechanisms of LBD, AD, and PD, which could benefit the understanding of the co-pathology as well as the potential treatment of these diseases simultaneously.
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http://dx.doi.org/10.1186/s12916-022-02404-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214990PMC
June 2022

Efficacy of a Self-Designed Questionnaire for Community Screening of COPD.

Int J Chron Obstruct Pulmon Dis 2022 14;17:1381-1391. Epub 2022 Jun 14.

Department of Respiratory and Critical Care Medicine, Shanghai Fifth People's Hospital, Fudan University, Shanghai, People's Republic of China.

Purpose: To evaluate the screening efficacy of a self-designed questionnaire for chronic obstructive pulmonary disease (COPD) and the potential gender disparity in its efficacy.

Patients And Methods: A screening questionnaire, the COPD Screening Questionnaire-Minhang (COPD-MH), was designed with reference to the self-scored COPD population screener (COPD-PS) and the COPD screening questionnaire (COPD-SQ), incorporating characteristics of the local population in Shanghai, China. The revised questionnaire included only five questions. Each question scored 0-4, with a highest total score of 20. The COPD-PS and COPD-SQ comprised 5 and 7 questions, respectively. Their scoring criteria were not consecutive integers and, thus, not easily counted. The COPD-MH focused on symptoms, and each item was set the same answers for convenience. Screening for COPD was conducted among residents over 40 years old in a community in Shanghai using the three aforementioned questionnaires. Each participant also received spirometry tests. A receiver operator characteristic (ROC) curve was drawn, and the area under the curve (AUC) was calculated to assess the validity of each questionnaire.

Results: A total of 1197 community residents in Minhang District completed the screening. A total of 1023 participants were finally included in analysis with a detected prevalence of 12.4% for COPD. The best cut-off values for the COPD-PS, COPD-SQ, and COPD-MH were 4, 16, and 7 points, respectively. The AUCs for these three questionnaires were >0.5, but the sensitivity of the COPD-MH was higher than those of the COPD-PS and COPD-SQ. The sensitivity of COPD-MH was 80.77% for males and 77.5% for females. The COPD-MH had higher diagnostic efficiency and higher sensitivity with gender-specific cut-off values.

Conclusion: The COPD-MH is comparable to and less time-consuming than the existing screening methods for COPD. Gender-related factors affect the optimal cut-off values of the COPD screening questionnaire, and rectifying this can improve the practical screening efficacy.
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http://dx.doi.org/10.2147/COPD.S359098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206516PMC
June 2022

The impact of son or daughter care on Chinese older adults' mental health.

Soc Sci Med 2022 Aug 1;306:115104. Epub 2022 Jun 1.

Oxford Institute of Population Ageing, University of Oxford, UK.

Rationale: The traditional cultural norm in China is that eldercare should be offered by a son and his family. However, several decades of family planning policies and rapid economic growth have led to low fertility rates, urbanisation, and cultural change, resulting in both reduced household size and the availability of sons. The traditional eldercare system is thus being challenged, especially in urban areas, with an increasing number of daughters supporting their parents, enabled by rising female empowerment, formal economic employment and improved financial capacities. Existing studies examine and compare the amount and types of support provided by sons and daughters, but very little is known about the differential health outcomes of parents experiencing eldercare offered by sons and by daughters.

Objective: This study investigates the difference in mental health outcomes of parents who receive eldercare from their sons and from their daughters.

Method: We analyse waves 2013, 2015, and 2018 of the China Health and Retirement Longitudinal Study, with mental health measured by a 10-item Center for Epidemiological Studies-Depression (CES-D-10) score. We compare the CES-D-10 score between parents with different sources of care - son's family, daughter's family or other - with multivariate statistical analysis.

Results: Receiving regular care from a son (or a daughter-in-law) is associated with a lower CES-D score than from a daughter (or a son-in-law). The difference mainly exists amongst those with a stronger son preference and those who are heavily deponent on their children, in particular rural parents, mothers, and those with less wealth.

Conclusion: Our study explores the evidence on mental health inequalities among parents with different sources of care, highlighting that lack of son care creates an apparent mental health disadvantage. Lowering older adults' dependency on their children may alleviate this inequality by improving their financial independence. We also suggest the development of a long-term care system and greater facilitation of older adults to take-up supplementary services for family care.
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http://dx.doi.org/10.1016/j.socscimed.2022.115104DOI Listing
August 2022

The Safety, Toleration, and Pharmacokinetics of Two Intravenous Voriconazole Formulations in Healthy Chinese Volunteers After Increasing Dose Administrations.

Clin Pharmacol Drug Dev 2022 Jun 16. Epub 2022 Jun 16.

Department of Clinical Pharmacology, Aerospace Center Hospital, Beijing, China.

Sulfobutyl ether-beta-cyclodextrin sodium salt contained in the marketed intravenous voriconazole injection as a solubilizer may cause harmful accumulations. This study aimed to evaluate the safety, tolerability, and pharmacokinetics (PKs) of two intravenous voriconazole formulations containing excipients from different manufacturers using increasing dose administrations in healthy Chinese volunteers. A randomized, double-blind, placebo-controlled trial was conducted in three cohorts with 42 healthy Chinese volunteers. Each cohort of 14 volunteers was allocated in proportion (8:4:2) to test the formulation, reference voriconazole, or placebo successively by single-dose then multiple-dose administrations of 3, 4, and 6 mg/kg. Forty-one volunteers completed all drug administrations. The pharmacokinetics of test formulations are characterized by high interindividual variability (coefficient of variance of C up to 68.0%, AUC up to 70.2%, and nonlinear PKs with a regression coefficient of C  = 1.31 and AUC  = 1.75 in a single dose). In the steady state, R of the test drug versus reference drug of the 3, 4, and 6 mg/kg dose group were 5.2 and 5.3, 5.6 and 6.3, and 5.8 and 5.5, respectively, and Rc were 2.5 and 2.7, 2.6 and 3.1, and 2.8 and 2.6, respectively. Eighty-three adverse events with 37 transient visual disturbances were mild. PKs with high interindividual variability, nonlinear characteristics, and significant dose-dependent accumulation were comparable between the two formulations. Overall, the safety of the test formulation was acceptable.
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http://dx.doi.org/10.1002/cpdd.1126DOI Listing
June 2022

Silk-based hydrogel incorporated with metal-organic framework nanozymes for enhanced osteochondral regeneration.

Bioact Mater 2023 Feb 31;20:221-242. Epub 2022 May 31.

Department of Orthopaedic Surgery, Institute of Digital Medicine, Nanjing First Hospital, Nanjing Medical University, 210006, Nanjing, China.

Osteochondral defects (OCD) cannot be efficiently repaired due to the unique physical architecture and the pathological microenvironment including enhanced oxidative stress and inflammation. Conventional strategies, such as the control of implant microstructure or the introduction of growth factors, have limited functions failing to manage these complex environments. Here we developed a multifunctional silk-based hydrogel incorporated with metal-organic framework nanozymes ([email protected]) to provide a suitable microenvironment for enhanced OCD regeneration. The incorporation of CuTA nanozymes endowed the SF hydrogel with a uniform microstructure and elevated hydrophilicity. In vitro cultivation of mesenchymal stem cells (MSCs) and chondrocytes showed that [email protected] hydrogel accelerated cell proliferation and enhanced cell viability, as well as had antioxidant and antibacterial properties. Under the inflammatory environment with the stimulation of IL-1β, [email protected] hydrogel still possessed the potential to promote MSC osteogenesis and deposition of cartilage-specific extracellular matrix (ECM). The proteomics analysis further confirmed that [email protected] hydrogel promoted cell proliferation and ECM synthesis. In the full-thickness OCD model of rabbit, [email protected] hydrogel displayed successfully in situ OCD regeneration, as evidenced by micro-CT, histology (HE, S/O, and toluidine blue staining) and immunohistochemistry (Col I and aggrecan immunostaining). Therefore, [email protected] hydrogel is a promising biomaterial targeted at the regeneration of OCD.
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http://dx.doi.org/10.1016/j.bioactmat.2022.05.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163388PMC
February 2023

Research progress on classical traditional Chinese medicine Taohong Siwu decoction in the treatment of coronary heart disease.

Biomed Pharmacother 2022 Aug 13;152:113249. Epub 2022 Jun 13.

College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shangdong Province 250355, China; Shandong Co-Innovation Center of Classic TCM Formula, Shandong University of Traditional Chinese Medicine, Jinan, Shangdong Province 250355, China. Electronic address:

The pathogenesis of coronary heart disease is closely related to blood stasis. Taohong Siwu decoction (THSW for short) is one of the most widely used prescriptions for activating blood and removing stasis. Clinical research has confirmed its curative effect on coronary heart disease. However, its underlying mechanism remains unclear. Therefore, this paper reviewed the clinical efficacy of THSW and determine its effective components based on a comprehensive literature review. Furthermore, the core components and targets of THSW in treating coronary heart disease using molecular docking were verified, and the interaction sites were predicted to construct a theoretical basis for the clinical application of THSW.
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http://dx.doi.org/10.1016/j.biopha.2022.113249DOI Listing
August 2022

Dynamics of Bacterial Composition and Association with Quality Formation and Biogenic Amines Accumulation during Fish Sauce Spontaneous Fermentation.

Appl Environ Microbiol 2022 07 13;88(13):e0069022. Epub 2022 Jun 13.

School of Food Science and Technology, Dalian Polytechnic Universitygrid.440692.d, Dalian, China.

High-throughput sequencing and high-pressure liquid chromatography (HPLC) methods were used to investigate the influences of microbial dynamics on the quality and biogenic amine (BA) content during fish sauce fermentation. The homogeneity of total viable bacteria and lactic acid bacteria in fish sauce becomes higher as fermentation progresses. was the key genus of fish sauce fermentation. (38.43%) and (41.01%) were the dominant genera in the samples fermented for 3 months and 18 months, respectively. These three bacterial genera were significantly related to the physicochemical characteristics and characteristic flavors of the sauces. was significantly positively correlated with nitrogen oxides, the main characteristic flavor components in fish sauce. The BA content in fish sauce fermentation increased from 106.88 to 376.03 mg/kg, and the content of histamine reached 115.30 mg/kg at the end of fermentation, indicating that fish sauce has health risks. About 66.67% of isolates were able to produce a large amount of BA, suggesting that was the key genus for BA accumulation in fish sauce fermentation. Research on reducing the content of BA in fish sauce by intervening with regard to the fermentation temperature showed that a safe fish sauce product could be obtained at the fermentation temperature of about 25°C. These results help us to understand the contribution of microbial community composition to fish sauce fermentation and provide a basis for improving the quality and safety of fermented fish sauce. Traditional fermentation of fish sauce is mainly carried out by complex microbial communities from raw anchovies and processing environments. However, it is still unclear how the environmental microbiota influences the quality and the safety of fish sauce products. Therefore, this study comprehensively explained the influence of microorganisms on the quality and safety of fish sauce during the fermentation process in terms of physicochemical characters, flavors, and BA. Additionally, the accumulation of BA in fish sauce fermentation was controlled by intervening in the fermentation temperature. This finding contributes to a deeper understanding of the role of environmental microbiota during fermentation and provides data support for improving the safety of fish sauce.
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http://dx.doi.org/10.1128/aem.00690-22DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275223PMC
July 2022
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