Publications by authors named "Yanan Yang"

181 Publications

Recognition of key factors on attached microalgae growth from the internal sight of biofilm.

Sci Total Environ 2021 Nov 4:151417. Epub 2021 Nov 4.

College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266590, China; Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science & Engineering, Shandong University, Qingdao 266237, China.

Microalgae cultivation with wastewater could realize the advanced water treatment and pollutant conversion to biomass. Attached microalgae cultivation mode, that can avoid the high-cost and energy-extensive consumption process of biomass recovery from water in suspended cultivation mode, is getting increasing attention. During the attached cultivation, light and nutrient concentration in the internal biofilm, play a direct and crucial role in regulating the growth of microalgae. Hence, the distribution of light and nutrients at different depths of biofilm were first explored in this study together with the change rules of its internal distribution under different external nutrient levels. It demonstrated that the gross growth rate was enhanced by increasing the external nutrient level. Seen from the internal sight of biofilm, the internal nutrient level had a positive response to the external nutrient change. Nutrients (especially nitrogen) distributed homogeneously through the biofilm, and no serious nutrient starvation occurred at the surface layer of biofilm. Photosynthesis rate linearly decreased along the depth of microalgae biofilm (10-120 μm). In conclusion, light, rather than nutrient, would be the key influencing factor on attached microalgae growth. How to optimize the internal light distribution would determine the wastewater purification efficiency based on attached microalgae cultivation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.151417DOI Listing
November 2021

The Expression Regulatory Network in the Lung Tissue of Tibetan Pigs Provides Insight Into Hypoxia-Sensitive Pathways in High-Altitude Hypoxia.

Front Genet 2021 7;12:691592. Epub 2021 Oct 7.

College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.

To adapt to a low-oxygen environment, Tibetan pigs have developed a series of unique characteristics and can transport oxygen more effectively; however, the regulation of the associated processes in high-altitude animals remains elusive. We performed mRNA-seq and miRNA-seq, and we constructed coexpression regulatory networks of the lung tissues of Tibetan and Landrace pigs. 2, and 1 were identified as major regulators of hypoxia-induced genes that regulate blood pressure and circulation, and they were enriched in pathways related to signal transduction and angiogenesis, such as -1, 3-, and . may promote the combination of hemoglobin and oxygen as well as angiogenesis for high-altitude adaptation in Tibetan pigs. The expression of 2 showed a similar tendency of alveolar septum thickness among the four groups. These results indicated that 2 activity may lead to widening of the alveolar wall and septum, alveolar structure damage, and collapse of alveolar space with remarkable fibrosis. These findings provide a perspective on hypoxia-adaptive genes in the lungs in addition to insights into potential candidate genes in Tibetan pigs for further research in the field of high-altitude adaptation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2021.691592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8529057PMC
October 2021

Mixed lineage kinase domain-like pseudokinase-mediated necroptosis aggravates periodontitis progression.

J Mol Med (Berl) 2021 Oct 13. Epub 2021 Oct 13.

Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Periodontics, School of Stomatology, Tongji University, Shanghai, China.

Necroptosis is a form of cell death that is reportedly involved in the pathogenesis of periodontitis. The role of Mlkl-involved necroptosis remains unclear. Herein, this project aimed to explore the role of MLKL-mediated necroptosis in periodontitis in vitro and in vivo. Expression of RIPK3, MLKL, and phosphorylated MLKL was observed in gingival tissues obtained from healthy subjects or patients with periodontitis. The cell viability of Porphyromonas gingivalis lipopolysaccharide (LPS-Pg)-treated cells was detected. In wild type or Mlkl deficiency mice with ligature-induced periodontitis, alveolar bone loss and osteoclast activation were assessed. mRNA levels of inflammatory cytokines in bone marrow-derived macrophages were tested by qRT-PCR. Increased expression of RIPK3, MLKL, and phosphorylated MLKL was observed in gingival tissues obtained from patients with periodontitis. Porphyromonas gingivalis lipopolysaccharide (LPS-Pg)-treated cells developed necroptosis after caspase inhibition and negatively regulated the NF-κB signaling pathway. In mice with ligature-induced periodontitis, Mlkl deficiency reduced alveolar bone loss and weakened osteoclast activation. Furthermore, genetic ablation of Mlkl in LPS-Pg-treated bone marrow-derived macrophages increased the mRNA levels of tumor necrosis factor-α, interleukin (Il)-1β, Il-6, cyclooxygenase 2, matrix metalloproteinase 9, and receptor activator of nuclear factor kappa-B ligand. Our data indicated that MLKL-mediated necroptosis aggravates the development of periodontitis in a Mlkl-deficient mouse. This will provide a new sight for the understanding of etiology and therapies of periodontitis. KEY MESSAGES: MLKL expression was up-regulated in inflamed human gingival tissue. Mlkl deficiency affected the progression of periodontitis. Necroptosis played a major role in mice periodontitis model. Knockout of Mlkl had a significant effect on inflammatory responses.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00109-021-02126-7DOI Listing
October 2021

Anticancer roles of let-7f-1-3p in non-small cell lung cancer via direct targeting of integrin β1.

Exp Ther Med 2021 Nov 16;22(5):1305. Epub 2021 Sep 16.

Key Laboratory of Tumor Molecular Biology, Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.

Lung cancer is one of the most common types of cancer, with the highest mortality rate worldwide. MicroRNAs play notable roles in the chemotherapeutic effects of anticancer drugs. The present study used reverse transcription-quantitative PCR, western blotting and cell migration and invasion assays to reveal the role of let-7f-1-3p in non-small cell lung cancer (NSCLC) and explore the effect of let-7f-1-3p on doxorubicin (DOX) treatment. It was demonstrated that the levels of let-7f-1-3p in carcinoma tissues were lower compared with those in paracarcinoma tissues. Thus, let-7f-1-3p may act as a suppressor gene. The present study also explored the role of let-7f-1-3p in A549 and NCI-H1975 cells. Results revealed that let-7f-1-3p could inhibit the viability, migration and invasion of NSCLC cells and induce their apoptosis. Integrin β1 acted as a target gene regulated by let-7f-1-3p. This suggested that let-7f-1-3p could enhance DOX-inhibited cell viability, migration and invasion . Overall, the present study demonstrated that let-7f-1-3p may act as a target for drug design and lung cancer therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/etm.2021.10740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461611PMC
November 2021

Vascular characteristics and expression of hypoxia genes in Tibetan pigs' hearts.

Vet Med Sci 2021 Sep 25. Epub 2021 Sep 25.

College of Animal Science & Technology, Gansu Agricultural University, Lanzhou, P.R. China.

Background: Tibetan pigs have exhibited unique characteristics from low-altitudes pigs and adapted well to the Qinghai-Tibet Plateau.

Objectives: The current study was undertaken to investigate the hypoxic adaptation of heart in Tibetan pigs.

Methods: The hearts of Tibetan pigs and Landrace pigs raised at high or low altitudes were compared using 3D casting technology, scanning electron microscopy and real-time quantitative PCR (qRT-PCR).

Results: We found that the ratio of the major axis to the minor axis and the density of the heart were significantly higher in Tibetan pigs than in Landrace pigs (p < 0.05). Tibetan pigs had larger diameters and higher densities of arterioles than Landrace pigs (p < 0.05), and these features have a similar variation with the expression of vascular endothelial growth factor (VEGF). The cardiac expression levels of hypoxia-inducible factor-1α (HIF-1α) and endothelial nitric oxide synthase (eNOS) were significantly higher in pigs reared at high altitudes than in those reared at low altitudes (p < 0.05). In contrast, Egl nine homolog 1 (EGLN1) had the opposite trend with respect to HIF-1α and eNOS and was related to red blood cell (RBC) counts. Notably, the expressions of erythropoietin (EPO) and endothelial PAS domain-containing protein 1 (EPAS1) were significantly higher in Landrace pigs kept at high altitudes than in the others (p < 0.05) and were associated with haemoglobin.

Conclusions: These findings show that the regulation of the heart function of Tibetan pigs in a hypoxic environment is manifested at various levels to ensure the circulation of blood under extreme environmental conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/vms3.639DOI Listing
September 2021

Real-world efficacy of osimertinib in previously EGFR-TKI treated NSCLC patients without identification of T790M mutation.

J Cancer Res Clin Oncol 2021 Aug 26. Epub 2021 Aug 26.

Division of Epidemiology, Department of Health Sciences Research, Mayo Clinic, 13400 E Shea Blvd, Scottsdale, AZ, USA.

Background: The efficacy of osimertinib in previously EGFR-TKI-treated NSCLC without identification of T790M mutational status remains unclear in real-world practice.

Patients And Methods: 417 patients had stage III-IV NSCLC harboring EGFR mutation and 154 out of 417 patients receiving osimertinib as ≥ second-line EGFR-TKI were identified. The time to treatment failure and risk of death were analyzed.

Results: Higher risk of death was found in EGFR-mutant patients with age ≥ 65 years, non-adenocarcinoma, no surgery or radiation, non-exon 19 deletion/exon 21 L858R, higher ECOG PS (2-4), PD-L1 expression ≥ 50%, and bone/liver/adrenal metastasis (all p < 0.05). Osimertinib as ≥ second-line TKI in patients with/without identification of T790M revealed lower risk of death compared to first-line first/second generation TKI without subsequent osimertinib (p = 0.0002; 0.0232, respectively). However, osimertinib-treated patients with T790M did not have superior survival than those without (p = 0.2803). A higher risk of treatment failure for osimertinib was found in males, patients with first-line TKI duration ≤ 12 months, BMI drop > 10%, and PD-L1 expression ≥ 50% (All p < 0.05). Nonetheless, osimertinib as ≥ second-line TKI in patients without identification of 790 M did not have higher risk of treatment failure than those with T790M (p = 0.1236).

Conclusions: This study demonstrates that osimertinib as second line or subsequent TKI in EGFR-TKI-treated patients without identification of T790M revealed lower risk of death compared to first-line first/second generation TKI without subsequent osimertinib, in real-world practice. Additionally, EGFR-mutant patients with PD-L1 expression ≥ 50% had a higher risk of treatment failure for osimertinib and worse overall survival than those with PD-L1 expression < 50%. These results suggest that osimertinib as second line or subsequent TKI may be a potential alternative option for the treatment of patients without identification of T790M and PD-L1 expression ≥ 50% is associated with a significantly poor outcome in patients receiving osimertinib.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00432-021-03766-5DOI Listing
August 2021

Low Cd-accumulating rice intercropping with Sesbania cannabina L. reduces grain Cd while promoting phytoremediation of Cd-contaminated soil.

Sci Total Environ 2021 Dec 10;800:149600. Epub 2021 Aug 10.

College of Natural Resources and Environment, South China Agricultural University / Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture of the People's Republic of China, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Eco-Circular Agriculture / Guangdong Engineering Research Center for Modern Eco-agriculture and Circular Agriculture, Guangzhou 510642, China. Electronic address:

Paddy field pollution with Cd has become a serious problem and poses threat to public health. Intercropping is new good agricultural practice for phytoremediation in Cd contaminated soil. Field and pot experiments were conducted to examine the effects of intercropping low Cd-accumulating rice with Sesbania cannabina on plant growth, uptake of Cd by the intercropping system, and rhizosphere microecology, and to evaluate the potential remediation of Cd contaminated soil and safety production of rice. The results of in the field experiment show that, in intercropping system, the concentration of Cd in the grain of rice (0.18 mg kg) was below the threshold level permitted by the National Food Safety Standard of China (GB 2762-2017, 0.20 mg kg). Furthermore, the yield per plant of rice in intercropping system significantly (P < 0.05) increased by 19.71%. At the same time, the bio-concentration amount (BCA) of Cd per plant of Sesbania cannabina in intercropping system significantly (P < 0.05) increased by 46.15%. The metal removal equivalent ratio (MRER) of Cd was 1.11, indicating that the intercropping system had advantage in Cd removal. In the pot experiment, the rice intercropped with Sesbania cannabina under no barrier (IN) treatment significantly (P < 0.05) decreased the content of rhizosphere organic acids (oxalic and malic acids), and significantly (P < 0.05) increased the rhizosphere pH value and total iron plaque concentration on the root surface compared to the intercropping with plastic barrier (IN+P) treatment, which could significantly (P < 0.05) decreased the available Cd content in rhizosphere soil and the accumulation of Cd in rice organs. With this study we demonstrated that lower rhizosphere organic acids and higher iron plaque can obstruct and decreased the Cd absorbed by rice in a rice-Sesbania cannabina intercropping system. We conclude that intercropping rice with Sesbania cannabina is a promising and cost-effective agricultural practice for safe crop production and for phytoremediation in Cd-contaminated paddy soil.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.149600DOI Listing
December 2021

Exendin-4 may improve type 2 diabetes by modulating the epigenetic modifications of pancreatic histone H3 in STZ-induced diabetic C57BL/6 J mice.

J Physiol Biochem 2021 Aug 19. Epub 2021 Aug 19.

Department of Microbiology and Bioengineering, College of Life Science, Anhui Medical University, Hefei, 230032, Anhui, China.

Type 2 diabetes (T2D) is a complicated systemic disease that might be improved by exendin-4, although the epigenetic role remains unclear. In the current study, C57BL/6 J mice were used to generate a T2D model, followed by treatment with exendin-4 (10 μg/kg). Histone H3K9 and H3K23 acetylation, H3K4 mono-methylation, and H3K9 di-methylation were explored by western blot analysis of pancreatic histone extracts. Real-time polymerase chain reaction (PCR) was used to examine the expression levels of pancreatic beta cell development-related genes, and chromatin immunoprecipitation (ChIP) was applied to analyze H3 and H3K9 acetylation, H3K4 mono-methylation, and H3K9 di-methylation in the promoter region of the pancreatic and duodenal homeobox 1 (Pdx1) gene. The results showed that total H3K9 di-methylation and H3K9 and H3K23 acetylation increased in pancreatic tissues of diabetic mice, whereas H3K4 mono-methylation was reduced. All of these changes could be abrogated by treatment with exendin-4. Our data indicated that T2D progression might be improved by exendin-4 treatment through the reversal of global pancreatic histone H3K9 and H3K23 acetylation, H3K4 mono-methylation, and H3K9 di-methylation. A better understanding of these epigenetic alterations may, therefore, lead to novel therapeutic strategies for T2D.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13105-021-00835-8DOI Listing
August 2021

Fully Soft Pressure Sensor Based on Bionic Spine-Pillar Structure for Robotics Motion Monitoring.

Soft Robot 2021 Aug 18. Epub 2021 Aug 18.

College of Electronics and Information Engineering, Shandong University of Science and Technology, Qingdao, China.

Soft and stretchable sensors are essential to the development of electronic skin, especially their potential applications in health care and intelligent robots, which have increasingly attracted attentions. Herein, inspired by the epidermal tissue hierarchy, we propose a high-sensitivity fully soft capacitive pressure sensor with bionic spine-pillar microstructure. Benefiting from the combination of the random microscale spines and the millimeter-sized pillar array prepared based on polydimethylsiloxane, the proposed sensor exhibits a well deformability, a high sensitivity up to 2.87 k/Pa at low-pressure range, and a broad linear pressure dynamic range from 5 Pa to 100 kPa. A simple equivalent circuit model was established to demonstrate the sensing mechanism and geometric effect. For practical application demonstrations, the sensor was utilized to monitor local subtle and large movements of the skin, such as finger bending, wrist bending, swallowing, and facial muscle movements. The sensor shows a conformality with human skin to follow the skin extension closely. Furthermore, the proposed sensing strategy can provide a distinguishable tactile feedback for controlling robot arm and soft claw in various tasks, illustrating its potential applications in robotics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/soro.2020.0147DOI Listing
August 2021

Characterization of Shallow Whole-Metagenome Shotgun Sequencing as a High-Accuracy and Low-Cost Method by Complicated Mock Microbiomes.

Front Microbiol 2021 30;12:678319. Epub 2021 Jul 30.

Pharmacology and Toxicology Research Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Characterization of the bacterial composition and functional repertoires of microbiome samples is the most common application of metagenomics. Although deep whole-metagenome shotgun sequencing (WMS) provides high taxonomic resolution, it is generally cost-prohibitive for large longitudinal investigations. Until now, 16S rRNA gene amplicon sequencing (16S) has been the most widely used approach and usually cooperates with WMS to achieve cost-efficiency. However, the accuracy of 16S results and its consistency with WMS data have not been fully elaborated, especially by complicated microbiomes with defined compositional information. Here, we constructed two complex artificial microbiomes, which comprised more than 60 human gut bacterial species with even or varied abundance. Utilizing real fecal samples and mock communities, we provided solid evidence demonstrating that 16S results were of poor consistency with WMS data, and its accuracy was not satisfactory. In contrast, shallow whole-metagenome shotgun sequencing (shallow WMS, S-WMS) with a sequencing depth of 1 Gb provided outputs that highly resembled WMS data at both genus and species levels and presented much higher accuracy taxonomic assignments and functional predictions than 16S, thereby representing a better and cost-efficient alternative to 16S for large-scale microbiome studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.678319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361837PMC
July 2021

Adipo-specific chemerin knockout alters the metabolomic profile of adipose tissue under normal and high-fat diet conditions: Application of an untargeted liquid chromatography-tandem mass spectrometry metabolomics method.

Biomed Chromatogr 2021 Dec 24;35(12):e5220. Epub 2021 Aug 24.

School of Kinesiology, Shanghai University of Sport, Shanghai, China.

To explore the metabolic effect of chemerin, adipose-specific chemerin knockout (adipo-chemerin ) male mice were established and fed with 5-week normal diet (ND) or high-fat diet (HFD), and then the glycolipid metabolism index was measured and epididymal adipose tissue metabolomics detected using untargeted LC-tandem mass spectrometry (LC-MS/MS). Under HFD, adipo-chemerin mice showed improved glycolipid metabolism (decreased total cholesterol, low-density lipoprotein-cholesterol, insulin and Homeostasis Model Assessment of Insulin Resistance) compared with flox (control) mice. Furthermore, orthogonal partial least squares-discriminant analysis score plots identified separation of metabolites between adipo-chemerin mice and flox mice fed ND and HFD. Under HFD, 28 metabolites were significantly enhanced in adipo-chemerin mice, and pathway enrichment analysis suggested strong relationship of the differential metabolites with arginine and proline metabolism, phenylalanine metabolism, and phenylalanine, tyrosine and tryptophan biosynthesis, which were directly or indirectly related to lipid metabolism, inflammation and oxidative stress. Under ND, taurine was increased in adipo-chemerin mice, resulting in taurine and hypotaurine metabolism and primary bile acid biosynthesis. In conclusion, the improved effect of chemerin knockdown on the glycolipid metabolism of HFD-feeding male mice might be associated with the increases in differential metabolites and metabolic pathways involved in lipid metabolism, inflammation and oxidative stress, which provided insights into the mechanism of chemerin from a metabolomics aspect.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.5220DOI Listing
December 2021

Characteristics of Tibetan pig lung tissue in response to a hypoxic environment on the Qinghai-Tibet Plateau.

Arch Anim Breed 2021 28;64(1):283-292. Epub 2021 Jun 28.

College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.

To adapt to the plateau environment, Tibetan pigs' lungs have developed a unique physiological mechanism during evolution. The vascular corrosion casting technique and scanning electron microscopy were used to understand arterial architecture. Blood physiological index and quantitative real-time PCR (qRT-PCR) were used for assessing whether the lung can regulate the body through anatomical, physiological and molecular mechanisms to adapt to hypoxic environments. Our study showed that the lungs of Tibetan pigs were heavier and wider and that the pulmonary arteries were thicker and branched and had a denser vascular network than those of Landrace pigs. The hemoglobin (HGB), mean corpuscular hemoglobin concentration (MCHC) values of high-altitude pigs were significantly higher than those of low-altitude pigs. The expression levels of - , , and , but not those of and , were significantly higher in the lungs of high-altitude pigs than in those from pigs at a lower altitude ( ). These findings and a comprehensive analysis help elucidate the pulmonary mechanism of hypoxic adaptation in pigs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5194/aab-64-283-2021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253108PMC
June 2021

Visible and infrared optical modulation of PSLC smart films doped with ATO nanoparticles.

Dalton Trans 2021 Jul;50(29):10033-10040

National Key Laboratory of Science and Technology on Advanced Composites in Special Environments, Harbin 150080, P. R. China. and Key Laboratory of Micro-systems and Micro-structures Manufacturing, Ministry of Education, Harbin 150080, P. R. China.

In recent years, smart windows have been gradually applied and developed in the fields of environmental protection, energy management, smart camouflage and display. With the continuous upgrading of market demand, smart windows with both visible and infrared band modulation functions are the future development trends. However, the optical modulation of smart windows is usually limited in the visible light band (380-780 nm). In this paper, we report a novel smart window by doping antimony doped tin oxide (ATO) nanoparticles (NPs) into polymer stabilized liquid crystal (PSLC) films. These films have the widest waveband modulation function among those reported so far, covering the visible and infrared regions (380-5500 nm). The transmittance of the as-prepared smart films can be changed reversibly from highly transparent (78.5%) state to a strong light scattering (10%) state in the visible region. In addition, due to the localized surface plasmon resonance of ATO NPs, up to 80.7% of the infrared invisible light can be effectively shielded. The significance of this research is to provide theoretical and technical support for the broadband optical modulation of novel smart windows.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1dt01575cDOI Listing
July 2021

The maintenance of microbial community in human fecal samples by a cost effective preservation buffer.

Sci Rep 2021 06 29;11(1):13453. Epub 2021 Jun 29.

Beijing QuantiHealth Technology Co., Ltd., Beijing, 100070, China.

In the burgeoning microbiome field, powerful sequencing approaches and accompanied bioanalytical methods have made tremendous contributions to the discoveries of breakthroughs, which favor to unravel the intimate interplay between gut microbiota and human health. The proper preservation of samples before being processed is essential to guarantee the authenticity and reliability of microbiome studies. Hence, the development of preservation methods is extremely important to hold samples eligible for the consequent analysis, especially population cohort-based investigations or those spanning species or geography, which frequently facing difficulties in suppling freezing conditions. Although there are several commercial products available, the exploration of cost-efficient and ready-to-use preservation methods are still in a large demand. Here, we performed shotgun metagenomic sequencing and demonstrated that microbial consortia in human fecal samples were substantially preserved within a temporary storage of 4 h, independent of the storage temperature. We also verified a previous reported self-made preservation buffer (PB buffer) could not only preserve fecal microbiota at room temperature up to 4 weeks but also enable samples to endure a high temperature condition which mimics temperature variations in summer logistics. Moreover, PB buffer exhibited suitability for human saliva as well. Collectively, PB buffer may be a valuable choice to stabilize samples if neither freezing facilities nor liquid nitrogen is available.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-92869-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242035PMC
June 2021

Identification of differentially abundant mRNA transcripts and autocrine/paracrine factors in oocytes and follicle cells of mud crabs.

Anim Reprod Sci 2021 Jul 2;230:106784. Epub 2021 Jun 2.

School of Marine Science, Ningbo University, Ningbo, China. Electronic address:

The late vitellogenic stage of the mud crab is characterized by large and obvious follicle cells as well as an enlarged oocyte nucleus and a prominent germinal vesicle (GV). The aim of this study was evaluation of functions of oocytes and follicle cells during meiosis as well as at identifying associated ovarian autocrine/paracrine factors using comparative transcriptomics. The results from the KEGG pathway analysis indicated DNA replication, nucleotide excision repair, spliceosome and the ribosome pathways were highly associated with oocyte maturation across both transcriptomes. In addition, there was a larger abundance of mRNA transcripts for cell cycle-related genes in the oocyte, as well as cyclin A, cyclin B and CKS1B in the GV than at the time of germinal vesicle breakdown (GVBD). These findings indicate these cell cycle-related genes might be involved in GVBD induction. Results when there was localization of ligands and the respective receptors of VEGF, TGFβ propeptide and BMP9/10 indicated these proteins might be autocrine/paracrine factors. Results from functional analysis of VEGF, TGFβ propeptide and BMP9/10 in oocyte maturation using RNA interference revealed that these proteins might be involved in oocyte maturation by regulating cyclin abundance. This is the first study on the functions of VEGF in oocyte maturation in invertebrates.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.anireprosci.2021.106784DOI Listing
July 2021

A non-integrated iPSC line (SDQLCHi042-A) from a boy suffering from familial combined hyperlipidemia with compound heterozygous mutations of lipoprotein lipase gene.

Stem Cell Res 2021 05 26;53:102313. Epub 2021 Mar 26.

Jinan Pediatric Research Institute, Qilu Children's Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China. Electronic address:

In this study, peripheral blood monouclear cells (PBMCs) were donated from a boy suffering from familial combined hyperlipidemia confirmed by clinical and genetic diagnosis, which carried compound heterozygous mutations of lipoprotein lipase (LPL) gene. The induced pluripotent stem cell (iPSC) was generated with non-integrated episomal vectors carrying OCT4, SOX2, KLF4, BCL-XL and C-MYC. The iPSCs presented the morphology of pluripotent cells, highly expressed mRNA and protein of pluripotent markers, excellent differentiation potency in vitro and normal karyotype, and bore LPL gene mutations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scr.2021.102313DOI Listing
May 2021

The Mitochondrial Response to DNA Damage.

Front Cell Dev Biol 2021 12;9:669379. Epub 2021 May 12.

Department of Immunology, School of Basic Medical Science, Anhui Medical University, Hefei, China.

Mitochondria are double membrane organelles in eukaryotic cells that provide energy by generating adenosine triphosphate (ATP) through oxidative phosphorylation. They are crucial to many aspects of cellular metabolism. Mitochondria contain their own DNA that encodes for essential proteins involved in the execution of normal mitochondrial functions. Compared with nuclear DNA, the mitochondrial DNA (mtDNA) is more prone to be affected by DNA damaging agents, and accumulated DNA damages may cause mitochondrial dysfunction and drive the pathogenesis of a variety of human diseases, including neurodegenerative disorders and cancer. Therefore, understanding better how mtDNA damages are repaired will facilitate developing therapeutic strategies. In this review, we focus on our current understanding of the mtDNA repair system. We also discuss other mitochondrial events promoted by excessive DNA damages and inefficient DNA repair, such as mitochondrial fusion, fission, and mitophagy, which serve as quality control events for clearing damaged mtDNA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2021.669379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149749PMC
May 2021

The Chinese mitten crab genome provides insights into adaptive plasticity and developmental regulation.

Nat Commun 2021 04 22;12(1):2395. Epub 2021 Apr 22.

Simon F. S. Li Marine Science Laboratory, School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China.

The infraorder Brachyura (true or short-tailed crabs) represents a successful group of marine invertebrates yet with limited genomic resources. Here we report a chromosome-anchored reference genome and transcriptomes of the Chinese mitten crab Eriocheir sinensis, a catadromous crab and invasive species with wide environmental tolerance, strong osmoregulatory capacity and high fertility. We show the expansion of specific gene families in the crab, including F-ATPase, which enhances our knowledge on the adaptive plasticity of this successful invasive species. Our analysis of spatio-temporal transcriptomes and the genome of E. sinensis and other decapods shows that brachyurization development is associated with down-regulation of Hox genes at the megalopa stage when tail shortening occurs. A better understanding of the molecular mechanism regulating sexual development is achieved by integrated analysis of multiple omics. These genomic resources significantly expand the gene repertoire of Brachyura, and provide insights into the biology of this group, and Crustacea in general.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-22604-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062507PMC
April 2021

Morphological and scanning electron microscopic study of the gonadal arterioles in the Tibetan sheep.

Anat Histol Embryol 2021 Jul 20;50(4):694-700. Epub 2021 Apr 20.

College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.

This research aimed at revealing the structural features of Tibetan sheep's testicular and ovarian vasculature and providing theoretical basis for Tibetan sheep's physiological adaptability in plateau and low-oxygen environments. Through scanning electron microscopy, corrosion casts of the feature of the gonad arterioles were observed and analysed. The results of this study found that centrifugal arteries, centrifugal and centripetal arterioles were distributed in a 'dendritic' fashion in the testis lobule, the ovarian arteries and arterioles, which had a spiral shape. Testicular Tibetan Sheep knot-like artery had a denser spiral structure, and there were more branches in the microarteries. On the testis lobule and arteriole of caput epididymidis, the arteriole surface shows the 'fusiform' indentation to be more shallower. The ovarian artery had a higher degree of helix; its surface was distributed like a 'bark-like' indentation; and the ring was narrower and denser. Nevertheless, the arteriole network had a high concentration and a wide number of branches, as that of the ovarian arteriole network which had more end branches, the surface of the arterioles of ovaries and testicles was scarred and denser. The studies indicate that the structural features of testicular lobule, epididymal arterioles and ovarian arterioles are more conducive to increase the oxygen supply to the gonads tissue and controlling blood flow velocity and metabolism to respond to high altitudes and low-oxygen environments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/ahe.12671DOI Listing
July 2021

Berberine, a potential prebiotic to indirectly promote Akkermansia growth through stimulating gut mucin secretion.

Biomed Pharmacother 2021 Jul 14;139:111595. Epub 2021 Apr 14.

Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. Electronic address:

Background: Akkermansia spp. plays important roles in maintenance of host health. Increasing evidence reveals that berberine (BBR) may exert its pharmacological effects via, at least partially, promotion of Akkermansia spp. However, how BBR stimulates Akkermansia remains largely unknown.

Purpose: In this study, we investigated the mechanism underlying the Akkermansia-promoting effect of BBR.

Materials And Methods: The effect of BBR on Akkermansia was assessed in BBR-gavaged mice and direct incubation. The influence of BBR on intestinal mucin production was determined by alcian-blue staining and real-time PCR. The feces were analysis by gas chromatography-time-of-flight mass spectrometry (GC-TOF/MS) metabolomics. The role of polyamines in BBR-elicited mucin secretion and Akkermansia growth was evaluated by administration of difluoromethylornithine (DFMO) in mice.

Results: Gavage of BBR dose-dependently and time-dependently increased the abundance of Akkermansia in mice. However, it did not stimulate Akkermansia growth in direct incubation, suggesting that BBR may promote Akkermansia in a host-dependent way. Oral administration of BBR significantly increased the transcription of mucin-producing genes and mucin secretion in colon. Untargeted metabolomics analysis showed that BBR increased polyamines production in feces which are known to stimulate goblet cell proliferation and differentiation, but treatment with eukaryotic polyamine synthase inhibitor DFMO did not abolish the stimulating effect of BBR on mucin secretion and Akkermansia growth, indicating that the gut bacteria-derived but not the host-derived polyamines may involve in the BBR-promoted Akkermansia growth.

Conclusions: Our results reveal that BBR is a promising prebiotic for Akkermansia, and it promotes Akkermansia growth via stimulating mucin secretion in colon.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2021.111595DOI Listing
July 2021

Research on microbial structures, functions and metabolic pathways in an advanced denitrification system coupled with aerobic methane oxidation based on metagenomics.

Bioresour Technol 2021 Jul 26;332:125047. Epub 2021 Mar 26.

College of Resources and Environmental Engineering, Key Laboratory of Karst Georesources and Environment, Ministry of Education, Guizhou University, Guiyang 500025, China; Guizhou Karst Environmental Ecosystems Observation and Research Station, Ministry of Education, Guiyang, Guizhou 550025, China.

Methanotrophs can oxidize methane as the sole carbon and energy, and the resulting intermediate products can be simultaneously utilized by coexistent denitrifying bacteria to remove the nitrogen, which named Aerobic Methane Oxidation Coupled to Denitrification (AME-D). In this paper, an AME-D system was built in an improved denitrification bio-filter, to analyze the nitrogen removal efficiency and mechanism. The maximum TN removal rate reached 95.05%. As shown in Raman spectroscopy, in the effluent wave crests generated by the symmetric expansion and contraction of NO disappeared, and the distortion of olefin CH and C-OH stretching of alcohols appeared. Metagenomics revealed Methylotenera and Methylobacter were the dominated methanotrophs. There was a completed methane and nitrogen metabolism pathway with the synergism of nxrAB, narGHI, nasAB, pmo-amoABC and mmo genes. Dissimilatory reduction pathway was the primary nitrate removal pathway. Moreover, Bradyrhizobium could participate in methane and nitrogen metabolism simultaneously.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2021.125047DOI Listing
July 2021

Efficacy and Safety of TangWang Prescription for Type 2 Non-Proliferative Diabetic Retinopathy: A Study Protocol for a Randomized Controlled Trial.

Front Pharmacol 2021 15;12:594308. Epub 2021 Mar 15.

Department of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

Diabetic retinopathy (DR) is one of the most common and severe microvascular complications of diabetes mellitus (DM), which results in blindness among adults worldwide. Presently, the efficacy of drug treatments for diabetic retinopathy (DR) is not satisfactory, thus urgently necessitating effective drug treatment measures. TangWang prescription (TWP) has been found to have retinal protection effects in previous clinical and basic research. However, there is a lack of rigorous, randomized, and controlled studies. This study aims to evaluate the efficacy and safety of TWP in delaying the development of DR. This study is a randomized, double-blind, placebo-controlled, parallel-group, multicenter clinical trial, consisting of 384 participants to be randomized in a 1:1 ratio in the treatment and control groups. Furthermore, the treatment and control groups will be administered the TangWang prescription and the placebo, respectively, each at a dose of one bag twice a day. The study period will last for 48 weeks. The primary outcome measure will be the changes in the degree of retinal microvascular lesions before and after treatment. The secondary outcome will be changes in the degree of hemangioma, microvascular bleeding, microvascular leakage, macular edema, and vision. All statistical tests will be two-sided, and a < 0.05 will be considered statistically significant. We hypothesize that the patients with DR will benefit from TangWang prescription, and in addition to the central random system and platform of dynamic information collection, the patients' conditions will be monitored, and the data collected for analysis. If successful, this study will provide evidence that the TWP formulation delays in the progression of DR. The design of this trial has been registered with the ClinicalTrials.gov (NCT03025399).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2021.594308DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005869PMC
March 2021

Traditional Chinese medicines differentially modulate the gut microbiota based on their nature (Yao-Xing).

Phytomedicine 2021 May 10;85:153496. Epub 2021 Feb 10.

Pharmacology and Toxicology Research Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China. Electronic address:

Background: Property theory is a unique principle guiding traditional Chinese medicine (TCM) that classifies various TCMs into four natures (hot, warm, cool, and cold) to reflect their medical actions on the human body. Despite successful application for thousands of years, characterizing the nature of medical TCMs by modern physiological indicators remains a challenge.

Purpose: In this study, we investigated the potential relationship between the nature of TCMs and their modulation of the gut microbiota.

Study Design: We selected twelve TCMs with hot, warm, cool, or cold natures that possess antidiarrheal effects. Their aqueous extracts were orally administered to C57BL/6 mice at a clinical dose for 4 weeks. The gut microbiota was measured by 16S rRNA-based metagenomics, and the correlation between microbial composition/function and TCM nature was analyzed.

Results: Antidiarrheal TCMs with different natures showed distinct impacts on the gut microbiota. Hot-natured TCMs had no influence on the gut microbiota, warm-natured TCMs had a moderate influence, cool-natured TCMs had a strong influence, and cold-natured TCMs substantially changed the structure of the gut microbial community. The abundance of Anaerotruncus, Tyzzerella and Ruminiclostridium steadily increased, while that of Ruminococcaceae_UCG-010, Parasutterella and Bifidobacterium continuously decreased as the herbal nature turned from cold to hot. Microbiome functional prediction for Cluster of Orthologous Groups (COG) of proteins and Kyoto Encyclopedia of Genes and Genomes (KEGG) categories showed that colder TCMs imposed a stronger influence on microbial functional repertoires. Specifically, the abundance of ABC transporters, key bacterial proteins involved in nutrient absorption and drug resistance, was gradually decreased by colder TCMs.

Conclusion: Our results demonstrated that the nature of TCMs could be reflected by their modulation of gut microbes. Cold TCMs may exert their antidiarrheal effects, at least partially, by modulating the gut microbiota, while hot TCMs may alleviate dysentery in other ways.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.phymed.2021.153496DOI Listing
May 2021

A two-dimensional MXene-supported metal-organic framework for highly selective ambient electrocatalytic nitrogen reduction.

Nanoscale 2021 Feb 1;13(5):2843-2848. Epub 2021 Feb 1.

State Key Laboratory of Fine Chemicals, School of Chemical Engineering, Dalian University of Technology, China.

The conversion of nitrogen into ammonia is crucial for human activities. The electrochemical synthesis of ammonia from nitrogen and water is a green process with great application prospects; to this end, much effort has been made to improve the catalytic activity and selectivity. Here, a Co-based metal-organic framework (MOF), that is, zeolitic imidazolate framework-67 (ZIF-67), supported on a TiC MXene (defined as [email protected]) was prepared via in situ growth. Due to the high porosity and large active surface area of the MOF and the superior conductivity of the TiC MXene, the composite could efficiently synthesize ammonia electrochemically. In particular, the prepared [email protected] catalyst exhibited an excellent NH yield (6.52 μmol h cm), significantly higher than those achieved by TiC and ZIF-67 (2.77 and 1.61 μmol h cm, respectively) alone, and good Faraday efficiency (20.2%) at -0.4 V (vs. the reversible hydrogen electrode). This study not only expands the application of the MXene family in the electrochemical nitrogen reduction reaction but also provides ideas for the development of high-performance electrocatalysts for NRR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0nr08744kDOI Listing
February 2021

Insight into the Dual Inhibition Mechanism of Corilagin against MRSA Serine/Threonine Phosphatase (Stp1) by Molecular Modeling.

ACS Omega 2020 Dec 15;5(51):32959-32968. Epub 2020 Dec 15.

College of Food Science and Engineering, Jilin University, Changchun 130062, China.

Serine/threonine phosphatase (Stp1) is known to be involved in the regulation of cysteine phosphorylation levels in many different pathways, such as virulence factor regulation in methicillin-resistant (MRSA). Therefore, Stp1 can be used as a potential target for inhibiting MRSA infection. In this study, using virtual screening, we found that corilagin, a natural compound, was screened as a potential Stp1 inhibitor. Then, the phosphatase assay exhibited high inhibitory activity against Stp1. On the basis of the enzyme kinetics experiment, we found that corilagin exhibited a dual inhibitory mechanism of competitive and allosteric inhibition. To further elucidate the mechanism of interaction between corilagin and Stp1, molecular dynamics (MD) simulations were performed on the Stp1-corilagin complex. Consistent with the mutagenesis assays and fluorescence quenching assays results, the competitive and allosteric binding sites of corilagin with Stp1 were identified. In the competitive binding site of Stp1, Asn162, Ile164, Tyr199, and Lys232 were found to play a key role in this complex. In the allosteric binding site, hydrophobic interaction was the main binding force. The Asn142, Val145, Leu146, Pro152, and Phe179 residues of Stp1 were found to play a critical role in the binding of corilagin with Stp1. In this study, we used MD simulation to reveal the ligand-protein interactions, providing a theoretical basis. This research work, thus, lays down the foundation for the development of new Stp1 inhibitors to be utilized in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.0c03955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774088PMC
December 2020

[Quantification of microbial DNA in laboratory environment during DNA extraction].

Sheng Wu Gong Cheng Xue Bao 2020 Dec;36(12):2541-2547

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.

Metagenomic sequencing provides a powerful tool for microbial research. However, traditional experimental DNA extraction process will inevitably mix with environmental microorganisms which float in the air. It is still unclear whether the mixed environmental microbial DNA will heavily affect the metagenomic results of samples with extremely low microbial content. In this study, we first collected environmental bacteria in the laboratory and quantified the mixed environmental microbial DNA content during DNA extraction based on a qPCR-based quantification assay. We then extracted DNA from pure water in order to determine the mixed microbial taxons during extraction under open environment. At last, we extracted total DNA from a skin sample in a Biosafety cabinet or under open laboratory environment, to assess the impact of the mixed environmental microorganisms on the metagenomic results. Our results showed that DNA extraction under open laboratory environment in Beijing region resulted in 28.9 pg contaminant, which may accout for 30% of total DNA amount from skin samples. Metagenomic analysis revealed that the main incorporated environmental taxons were Cutibacterium acnes and Escherichia coli. Tens of environmental bacteria were foisted in the skin DNA samples, which largely decreased the relative abundance of dominant species and thus deteriorated the result accuracy. Therefore, analyzing microbial composition of samples with extremely low DNA content should better performed under aseptic environment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13345/j.cjb.200526DOI Listing
December 2020

Molecular Identification, Transcriptome Sequencing and Functional Annotation of Pulex irritans.

Acta Parasitol 2021 Jun 3;66(2):605-614. Epub 2021 Jan 3.

Department of Pathogenic Biology and Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University, No.76 Yanta West Road, Xi'an, 710061, China.

Purpose: Pulex irritans are vectors of various zoonotic pathogens. However, molecular studies on P. irritans and flea-borne diseases are limited due to the lack of molecular data. This study aimed to conduct transcriptome sequencing, functional annotation, and pathogen analysis of P. irritans.

Methods: Fleas collected from a dog were identified morphologically and molecularly. RNA was extracted for transcriptome sequencing and functional annotation. Open reading frames (ORFs) of unigenes were confirmed by employing bioinformatics strategies, and maximum likelihood (ML) trees were reconstructed based on the highly expressed genes of ejaculation globulin-specific 3-like protein, salivary protein, and actin for phylogenetic relationship analysis.

Results: The obtained mitochondrial 16S rRNA gene sequences showed 99.71% of similarity with P. irritans obtained from GenBank database. Transcriptome sequencing generated 74,412 unigenes, of which 53,211 were functionally annotated. A total of 195 unigenes were assigned to fleas, of which 69 contained complete ORFs. Phylogenetic trees of both ejaculatory globulin and salivary protein genes demonstrated that P. irritans first clustered with Pulicidae sp., indicating the reliability of transcriptome data. It is noteworthy that 1070 unigenes were assigned to Hymenolepis microstoma and Dipylidium caninum, of which 62 contained complete ORFs. The phylogenetic tree of the actin gene showed that the unigenes had closer relationships with Echinococcus sp., suggesting the role of P. irritans as intermediate hosts of tapeworms.

Conclusion: The results of this study provide the possibility for functional exploration of important genes and lay foundations for the prevention and control of P. irritans and flea-borne diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11686-020-00296-xDOI Listing
June 2021

Porphyromonas gingivalis facilitated the foam cell formation via lysosomal integral membrane protein 2 (LIMP2).

J Periodontal Res 2021 Apr 29;56(2):265-274. Epub 2020 Dec 29.

Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Periodontics, School of Stomatology, Tongji University, Shanghai, China.

Objective: The involvement of lysosomal integral membrane protein 2 (LIMP2) in cholesterol transport and formation of foam cells under the infection of Porphyromonas gingivalis (P. gingivalis) is yet to be elucidated. The current study verified the role and explored the mechanism of LIMP2 in promoting foam cell formation by P. gingivalis.

Background: An association between periodontitis and atherosclerosis (AS) has been established. P. gingivalis is a key pathogen of periodontitis that promotes foam cell formation by regulating activities of CD36 scavenger receptors expressed on the macrophages. LIMP2, a member of CD36 superfamily, is involved in cholesterol efflux. However, whether LIMP2 is involved in the formation of foam cells promoted by P. gingivalis remains unclear.

Methods: The formation of foam cells was examined by Oil Red O staining. The knockdown of limp2 was identified by qRT-PCR. The accumulation of cholesterol was monitored by Cholesterol Assay Kit. The location of P. gingivalis was visualized by confocal microscopy. Cathepsin L activity was monitored with Magic Red Cathepsin L Assay Kit. The key genes and pathways in P. gingivalis-infected macrophages were explored by RNA sequencing. The protein level was investigated by Western blotting.

Results: Porphyromonas gingivalis increases foam cells formation and upregulates the expression of LIMP2 in foam cells. The knockdown of limp2 decreases the number of foam cells and increases cholesterol export, which is related to lysosomal functions. In addition, the interaction between LIMP2 and caveolin-1(CAV1) might contribute to this process, and NF-κB and JNK activity is required for increased expression of P. gingivalis-induced LIMP2.

Conclusions: This study suggested that LIMP2 is involved in the foam cells formation facilitated by P. gingivalis, which favors a close connection between periodontitis and atherosclerosis (AS).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jre.12812DOI Listing
April 2021

Porphyromonas gingivalis facilitated the foam cell formation via lysosomal integral membrane protein 2 (LIMP2).

J Periodontal Res 2021 Apr 29;56(2):265-274. Epub 2020 Dec 29.

Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Periodontics, School of Stomatology, Tongji University, Shanghai, China.

Objective: The involvement of lysosomal integral membrane protein 2 (LIMP2) in cholesterol transport and formation of foam cells under the infection of Porphyromonas gingivalis (P. gingivalis) is yet to be elucidated. The current study verified the role and explored the mechanism of LIMP2 in promoting foam cell formation by P. gingivalis.

Background: An association between periodontitis and atherosclerosis (AS) has been established. P. gingivalis is a key pathogen of periodontitis that promotes foam cell formation by regulating activities of CD36 scavenger receptors expressed on the macrophages. LIMP2, a member of CD36 superfamily, is involved in cholesterol efflux. However, whether LIMP2 is involved in the formation of foam cells promoted by P. gingivalis remains unclear.

Methods: The formation of foam cells was examined by Oil Red O staining. The knockdown of limp2 was identified by qRT-PCR. The accumulation of cholesterol was monitored by Cholesterol Assay Kit. The location of P. gingivalis was visualized by confocal microscopy. Cathepsin L activity was monitored with Magic Red Cathepsin L Assay Kit. The key genes and pathways in P. gingivalis-infected macrophages were explored by RNA sequencing. The protein level was investigated by Western blotting.

Results: Porphyromonas gingivalis increases foam cells formation and upregulates the expression of LIMP2 in foam cells. The knockdown of limp2 decreases the number of foam cells and increases cholesterol export, which is related to lysosomal functions. In addition, the interaction between LIMP2 and caveolin-1(CAV1) might contribute to this process, and NF-κB and JNK activity is required for increased expression of P. gingivalis-induced LIMP2.

Conclusions: This study suggested that LIMP2 is involved in the foam cells formation facilitated by P. gingivalis, which favors a close connection between periodontitis and atherosclerosis (AS).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jre.12812DOI Listing
April 2021

Electrocatalytic Synthesis of Ammonia Using a 2D Ti C MXene Loaded with Copper Nanoparticles.

Chempluschem 2021 Jan 20;86(1):166-170. Epub 2020 Nov 20.

Department of Materials Science and Engineering, China Jiliang University, Hangzhou, 310018, P. R. China.

As an energy-saving and environmentally friendly ammonia synthesis method, electrocatalytic nitrogen reduction reaction (NRR) has received a great deal of attention. There is thus an urgent need to find high-efficiency electrocatalysts for the NRR. In this work, a Cu/Ti C composite catalyst was prepared and demonstrated excellent selectivity under environmental conditions, which could efficiently convert N into NH electrochemically. In 0.1 M KOH, Cu/Ti C can achieve a high Faradaic efficiency of 7.31 % and a high NH production rate of 3.04 μmol h  cm at -0.5 V vs. RHE. Moreover, the material also exhibits superior electrochemical stability and durability. At the same time, density functional theory (DFT) shows that, compared with Ti C , Cu/Ti C exhibits a wider conduction and valence band and a larger Fermi level, thus indicating that Cu plays a vital role in the enhancement of the catalytic activity and conductivity of Ti C -based materials. This work provides a feasible strategy for designing high-efficiency MXene-based NRR electrocatalysts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cplu.202000702DOI Listing
January 2021
-->