Publications by authors named "Yan-Feng Lin"

6 Publications

  • Page 1 of 1

A new species of the Genus Pelodiscus Fitzinger, 1835 (Testudines: Trionychidae) from Huangshan, Anhui, China.

Zootaxa 2021 Oct 28;5060(1):137-145. Epub 2021 Oct 28.

Anhui Province Key Laboratory of the Conservation and Exploitation of Biological Resource, College of Life Sciences, Anhui Normal University, Wuhu, 241000, China.

A new species of the soft-shelled turtle genus Pelodiscus is described based on seven specimens from Huangshan, southern Anhui Province, China. The new species, Pelodiscus huangshanensis sp. nov., is distinguished from other species in the genus Pelodiscus by the following characteristics: (1) Small size (maximum carapace length of 101.16 mm and maximum body length of 190 mm); (2) keel high; (3) tiny yellowish-white spots on the throat; (4) no black pinstripes around the eyes; (5) white longitudinal bands on both sides of the neck in juveniles, absent in adults; (6) plastron yellowish-white, and only a dark patch on each side of the armpit; (7) many tubercles on the dorsal surface, but indistinct in the center; and (8) entoplastron ⌒ shaped. The phylogenetic relationships of the species in Pelodiscus were reconstructed using the sequences of cytochrome b (cyt b) and NADH dehydrogenase subunit 4 (ND4) genes. The new species formed a monophyletic clade with strong support. The uncorrected pairwise distances between the new species and other representatives of Pelodiscus ranged from 5.4% to 9.2% for cyt b and 4.1% to 7.6% for ND4. The new species brings the number of species of the genus Pelodiscus to six; five species are distributed in China, with three species endemic to China.
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October 2021

Forensic nanopore sequencing of STRs and SNPs using Verogen's ForenSeq DNA Signature Prep Kit and MinION.

Int J Legal Med 2021 Sep 5;135(5):1685-1693. Epub 2021 May 5.

School of Forensic Medicine, Shanxi Medical University, Taiyuan, 030001, People's Republic of China.

The MinION nanopore sequencing device (Oxford Nanopore Technologies, Oxford, UK) is the smallest commercially available sequencer and can be used outside of conventional laboratories. The use of the MinION for forensic applications, however, is hindered by the high error rate of nanopore sequencing. One approach to solving this problem is to identify forensic genetic markers that can consistently be typed correctly based on nanopore sequencing. In this pilot study, we explored the use of nanopore sequencing for single nucleotide polymorphism (SNP) and short tandem repeat (STR) profiling using Verogen's (San Diego, CA, USA) ForenSeq DNA Signature Prep Kit. Thirty single-contributor samples and DNA standard material 2800 M were genotyped using the Illumina (San Diego, CA, USA) MiSeq FGx and MinION (with R9.4.1 flow cells) devices. With an optimized cutoff for allelic imbalance, all 94 identity-informative SNP loci could be genotyped reliably using the MinION device, with an overall accuracy of 99.958% (1 error among 2926 genotypes). STR typing was notably error prone, and its accuracy was locus dependent. We developed a custom-made bioinformatics workflow, and finally selected 13 autosomal STRs, 14 Y-STRs, and 4 X-STRs showing high consistency between nanopore and Illumina sequencing among the tested samples. These SNP and STR loci could be candidates for panel design for forensic analysis based on nanopore sequencing.
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September 2021

The complete mitochondrial genome of (Testudines: Trionychidae).

Mitochondrial DNA B Resour 2019 Jul 10;4(2):2154-2155. Epub 2019 Jul 10.

College of Life and Environment Sciences, Huangshan University, Huangshan, China.

The complete mitochondrial genome sequence of was determined by shotgun sequencing. The total length of mitogenome is 16,593 bp, and contains 13 protein-coding genes, 22 tRNA genes, 2 ribosome RNA genes, and 1 control region. Most of the genes of . were distributed on the H-strand, except for the ND6 subunit gene and eight tRNA genes which were encoded on the L-strand. The phylogenetic tree of . and 11 other closely related species was reconstructed. The phylogenetic analyses based on these mitogenomes presented here will be useful for further insights on the evolutionary relationships of Trionychidae.
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July 2019

[Case analysis and control strategy of imported malaria in Qingcheng District, Qingyuan City from 2011 to 2016].

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi 2017 Oct;29(6):743-745

Qingcheng District Center for Disease Control and Prevention, Guangdong Province, Qingyuan 511500, China.

Objective: To understand the characteristics of imported malaria cases in Qingcheng District, Qingyuan City and explore the strategies and priorities in prevention and control, so as to provide the evidence for improving the diagnosis, treatment and management of imported malaria.

Methods: The data of imported malaria as well as the case epidemiological investigations were collected and retrospectively analyzed for the species composition, original countries, population distribution, regional distribution, onset situation, diagnosis, treatment, etc. in Qingcheng District from 2011 to 2016.

Results: The number of imported malaria cases was 13 from 2011 to 2016. All the patients were confirmed by laboratory, and of which, 9 patients infected with , 1 with , 1 with and 2 with mixed infections (). The yearly incidence of imported malaria presented an uptrend. The infection sources of all the patients were from African countries, and the exported labor workers and travelers for business from malaria endemic areas were the high risk population. The reported time was mainly January, February, November and December (11/13, 84.62%). All the patients were male, and the majority of them (12/13, 2.31%) were 21-60 years old. The median time from onset to seeing a doctor was 2.5 days and the median time from seeing a doctor to being diagnosed was 1.9 day. Six patients (46.15%) were diagnosed as other diseases at the first visit to a doctor, and one patient died of falciparum malaria because of delayed diagnosis.

Conclusions: The incidence of overseas imported malaria presents an uptrend in Qingcheng District. It is necessary to further strengthen the professional training in medical staff to improve the diagnosis and treatment of malaria cases. It is also necessary to strengthen the multisectoral cooperation, establish the surveillance in the high risk population, etc.
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October 2017

[The protective effect of low molecular weight heparin on nephropathy in rats with pregnancy induced hypertension].

Xin Yu Yan feng Lin

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2005 May;21(2):212-5

Department of Gynecology and Obstetrics, The Second Affiliated Hospital, China Medical University, Shengyang 110004, China.

Aim: To investigate the protective effect of low molecular weight heparin (LMWH) on nephropathy in rats with pregnancy induced hypertension and to study its possible mechanism.

Methods: The levels of the expression of renal ERK1/2 protein and mRNA were detected in PIH rats which were made by injection of L-NAME, normal pregnant rats and rats treated with LMWH by immunohistochemistry, Western blotting and reverse transcription-polymerase chain reaction(RT-PCR). The renal tissue was observed by using light microscopy.

Results: The expression level of renal ERK1/2 protein and mRNA in LMWH-treated rats were significantly lower than that in PIH rats, while the expression level of renal ERK1/2 protein and mRNA in PIH rats was significantly higher than that in normal pregnant rats (P < 0.01), the intensity of ERK1/2 expression had no obvious differences among 3 groups. The average arterial pressure and urine protein in LMWH group were decreased, but no decrease was observed in normal rats. Mesangial expansion and basal membrane thickening were obviously retarded in LMWH- treated group.

Conclusion: LMWH has renal protective effect on PIH rats, whose mechanism may be associated partly with a down-regulation of ERK expression.
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May 2005