Publications by authors named "Yan Zhu"

1,771 Publications

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Changes in the prevalence of hyperuricemia in clients of health examination in Eastern China, 2009 to 2019.

BMC Endocr Disord 2022 Aug 10;22(1):202. Epub 2022 Aug 10.

Clinical Medical College, Yangzhou University, Yangzhou, 225001, Jiangsu, China.

Background: With the continuous improvement of people's living standards, the incidence of hyperuricemia (HUA) is increasing globally. The prevalence of HUA ranged in terms of region, race, and age. This study aims to investigate the changes in the prevalence of HUA in clients of health examination in Eastern China between 2009 and 2019.

Methods: Chinese men and women aged 20-79 years (n = 4847 in the 2009 group and n = 12,188 in 2019 group) who had received health examinations were enrolled. Serum uric acid (UA) levels and biochemical parameters, including fasting blood-glucose (FBG), triglyceride (TG), total cholesterol (CHOL), high-density lipoprotein (HDL), low-density lipoprotein (LDL), creatinine (Cr) and blood urea nitrogen (BUN) were evaluated. The prevalence of HUA in different age groups were measured, and the correlation of biochemical parameters with HUA were analyzed.

Results: The prevalence of HUA was 18.7% in the 2019 group, which was significantly higher than that in 2009 (11.1%). In females, the prevalence of HUA was significantly higher in 2019 than 2009 for age groups of 20-29 and 30-39 years. In male population, 2019 participants had significantly higher age-specific prevalence for all age groups than 2009 participants. Young men aged 20-29 years became the main population of HUA in the 2019 participants, whereas middle-aged men aged 40-49 years had the highest prevalence of HUA in the 2009 participants. The prevalence rates of HUA in all BMI groups in 2019 participants were significantly higher than those in 2009 participants. Spearmen's correlation analysis and Logistic regression analysis indicated that BMI was positively correlated with HUA. The receiver-operating characteristic curve (ROC) analysis showed BMI > 24.48 kg/m2 and BMI > 23.84 kg/m2 displayed good capacities to discriminate the population with HUA from those without HUA in 2009 and 2019 participants, respectively.

Conclusions: In recent 10 years, the prevalence of HUA was increased rapidly in Chinese adults, especially in males. In 2019, the young male group (20-29 years old) replaced the middle-aged male group (40-49 years old) in 2009 as the leading age group for male HUA. BMI was positively correlated with HUA, and might be a potential risk factors to predict HUA.
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http://dx.doi.org/10.1186/s12902-022-01118-zDOI Listing
August 2022

Calcimycin Inhibits and by Targeting the Prp8 Intein Splicing.

ACS Infect Dis 2022 Aug 10. Epub 2022 Aug 10.

Department of Pharmacology and Toxicology, College of Pharmacy, The University of Arizona, Tucson Arizona 85721-0207, United States.

Drug resistance is a significant concern in the treatment of diseases, including cryptococcosis caused by () and (). Alternative drug targets are necessary to overcome drug resistance before it attains a critical stage. Splicing of inteins from pro-protein precursors is crucial for activities of essential proteins hosting intein elements in many organisms, including human pathogens such as and . Through a high-throughput screening, we identified calcimycin (CMN) as a potent Prp8 intein splicing inhibitor with a minimum inhibitory concentration (MIC) of 1.5 μg/mL against the wild-type H99 (-WT or ). In contrast, CMN inhibited the intein-less mutant strain (-Mut) with a 16-fold higher MIC. Interestingly, and a few species were resistant to CMN. Further studies indicated that CMN reduced virulence factors such as urease activity, melanin production, and biofilm formation in . CMN also inhibited intracellular infection in macrophages. In a target-specific split nanoluciferase assay, the IC of CMN was 4.6 μg/mL. Binding of CMN to recombinant Prp8 intein was demonstrated by thermal shift assay and microscale thermophoresis. Treating cells with CMN reduced intein splicing. CMN was fungistatic and showed a synergistic effect with the known antifungal drug amphotericin B. Finally, CMN treatment at 20 mg/kg body weight led to 60% reduction in lung fungal load in a cryptococcal pulmonary infection mouse model. Overall, CMN represents a potent antifungal with a novel mechanism of action to treat and possibly infections.
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http://dx.doi.org/10.1021/acsinfecdis.2c00137DOI Listing
August 2022

TRIM family proteins: roles in proteostasis and neurodegenerative diseases.

Open Biol 2022 Aug 10;12(8):220098. Epub 2022 Aug 10.

Shenzhen Laboratory of Tumor Cell Biology, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China.

Neurodegenerative diseases (NDs) are a diverse group of disorders characterized by the progressive degeneration of the structure and function of the central or peripheral nervous systems. One of the major features of NDs, such as Alzheimer's disease (AD), Parkinson's disease (PD) and Huntington's disease (HD), is the aggregation of specific misfolded proteins, which induces cellular dysfunction, neuronal death, loss of synaptic connections and eventually brain damage. By far, a great amount of evidence has suggested that TRIM family proteins play crucial roles in the turnover of normal regulatory and misfolded proteins. To maintain cellular protein quality control, cells rely on two major classes of proteostasis: molecular chaperones and the degradative systems, the latter includes the ubiquitin-proteasome system (UPS) and autophagy; and their dysfunction has been established to result in various physiological disorders including NDs. Emerging evidence has shown that TRIM proteins are key players in facilitating the clearance of misfolded protein aggregates associated with neurodegenerative disorders. Understanding the different pathways these TRIM proteins employ during episodes of neurodegenerative disorder represents a promising therapeutic target. In this review, we elucidated and summarized the diverse roles with underlying mechanisms of members of the TRIM family proteins in NDs.
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http://dx.doi.org/10.1098/rsob.220098DOI Listing
August 2022

Synthesis of silver nanoparticles using extract and their potential biological function in cosmetics.

Heliyon 2022 Aug 29;8(8):e10021. Epub 2022 Jul 29.

The Center for Ion Beam Bioengineering and Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China.

Silver nanoparticles (AgNPs) synthesized from plant extracts have recently emerged as a rapidly growing field with numerous applications in pharmaceutical and clinical contexts. The purpose of this research is to come up with a novel method for the biosynthesis of silver nanoparticles that use leaf extract as a reducing agent. The synthesis of AgNPs was confirmed using UV-vis spectroscopy, and the properties of AgNPs were characterized using Transmission Electron Microscope, Fourier Infrared Spectrometer, X-ray diffraction, Thermogravimetric Analysis, and Zeta potential. The results showed that the AgNPs exhibited a characteristic absorption peak at 430 nm, their diameter ranged from 4 nm to 52 nm, and C, O, and Cl elements, which might represent flavonoids and phenolic components absorbed on the surface of AgNPs. The zeta potential of AgNPs was found to be -30.5 mV, which indicates repulsion among AgNPs and they have good dispersion stability. AgNPs have been found to suppress the tyrosinase activity both in mushroom tyrosinase and A375 cells, as well as diminish ROS formation in HaCat cells. According to this study, AgNPs is a novel material that can enhance skin health by preventing melanin development.
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http://dx.doi.org/10.1016/j.heliyon.2022.e10021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356174PMC
August 2022

Retraction notice to "Protective role of long noncoding RNA CRNDE in myocardial tissues from injury caused by sepsis through the microRNA-29a/SIRT1 axis" [Life Sci. 255 (2020) 117849].

Life Sci 2022 08 5:120814. Epub 2022 Aug 5.

Department of Infectious Diseases, Zaozhuang Municipal Hospital, Zaozhuang 277102, Shandong, PR China.

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http://dx.doi.org/10.1016/j.lfs.2022.120814DOI Listing
August 2022

Research Progress on Small Molecules Inhibitors Targeting TRK Kinases.

Curr Med Chem 2022 Aug 1. Epub 2022 Aug 1.

College of Pharmacy of Liaoning University, Shenyang, Liaoning 110036, P. R. China.

Background: Trk gene fusions are an important driver in the development of cancers including secretory breast cancer and infantile congenital sarcoma. Since the first generation of small molecule Trk inhibitors (Larotrectinib and Entrectinib) came to market, research on small molecule TRK inhibitors, especially second-generation inhibitors that break through the resistance problem, has developed rapidly. Therefore, this article focuses on the research progress of first-generation drugs and second-generation drugs that breakthrough drug resistance.

Methods: We use the database to search for relevant and cutting-edge documents, and then filter and select it based on the content. The articles are analyzed and classified, and finally, write articles according to themes.

Result: The phenomenon of Trk protein fusion and its relation to tumors is first described, followed by an explanation of the composition and signaling pathways of Trk kinases. The representative Trk inhibitors and the development of novel Trk inhibitors are classified according to whether they overcome drug resistance problems.

Conclusion: This paper provides a theoretical reference for the development of novel inhibitors by introducing and summarizing the representative and novel Trk inhibitors that break through the drug resistance problem.
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http://dx.doi.org/10.2174/0929867329666220801145639DOI Listing
August 2022

Clinical and Microbiological Characteristics of Bloodstream Infection in a Chinese Hospital: Hypervirulent and Multiclonal.

Infect Drug Resist 2022 26;15:3981-3990. Epub 2022 Jul 26.

Department of Infectious Diseases, The First Hospital of Quanzhou Affiliated to Fujian Medical University, Quanzhou, People's Republic of China.

Purpose: Hypervirulent (hvKP) is emerging globally and can cause various infections. This study aimed to investigate the clinical and microbiological characteristics of bloodstream infection (BSI) caused by hvKP.

Patients And Methods: The clinical data of hospitalized patients with BSI were retrospectively analyzed. The strains were collected and re-identified, and antimicrobial susceptibility testing was performed using the broth microdilution method. Capsular serotypes and virulence genes were detected using polymerase chain reaction, and hvKP was defined as aerobactin positive. Molecular typing was done by multilocus sequence typing. The hvKP and classic (cKP) subgroups were compared.

Results: Of the 66 nonrepetitive BSI strains included, 29 (43.9%) were hvKP. In these BSI hvKP strains, salmochelin and yersiniabactin accounted for 86.2% and 72.4%, respectively. The prevalence of cluster, , and was 89.7%, 86.2%, 72.4%, 51.7%, and 41.4%, respectively, which were all significantly different between the hvKP and cKP subgroups. Serotypes K1 and K2 were strongly associated with hypervirulence ( < 0.05). Nineteen sequence types were scattered in the 29 hvKP strains, and the most common was ST23 (24.1%). None of the hvKP strains were carbapenem resistant. Compared with cKP, hvKP was more capable of developing a liver abscess. However, the 30-day mortality rate was lower (13.8% vs 21.6%) in the hvKP subgroup than in the cKP subgroup.

Conclusion: This study demonstrated a high proportion of hvKP in BSI , most of which were RmpA and siderophore producing, and of multiclonal origin.
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http://dx.doi.org/10.2147/IDR.S371477DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343176PMC
July 2022

Docetaxel-loaded M1 macrophage-derived exosomes for a safe and efficient chemoimmunotherapy of breast cancer.

J Nanobiotechnology 2022 Aug 2;20(1):359. Epub 2022 Aug 2.

NICM Health Research Institute, Western Sydney University, Westmead, NSW, 2145, Australia.

The conversion of tumor-promoting M2 macrophage phenotype to tumor-suppressing M1 macrophages is a promising therapeutic approach for cancer treatment. However, the tumor normally provides an abundance of M2 macrophage stimuli, which creates an M2 macrophage-dominant immunosuppressive microenvironment. In our study, docetaxel (DTX) as chemotherapeutic modularity was loaded into M1 macrophage-derived exosomes (M1-Exo) with M1 proinflammatory nature to establish DTX-M1-Exo drug delivery system. We found that DTX-M1-Exo induced naïve M0 macrophages to polarize to M1 phenotype, while failed to repolarize to M2 macrophages upon Interleukin 4 restimulation due to impaired mitochondrial function. This suggests that DTX-M1-Exo can achieve long-term robust M1 activation in immunosuppressive tumor microenvironment. The in vivo results further confirmed that DTX-M1-Exo has a beneficial effect on macrophage infiltration and activation in the tumor tissues. Thus, DTX-M1-Exo is a novel macrophage polarization strategy via combined chemotherapy and immunotherapy to achieve great antitumor therapeutic efficacy.
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http://dx.doi.org/10.1186/s12951-022-01526-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344780PMC
August 2022

Electrochemical Aerobic Oxygenation and Nitrogenation of Cyclic Alkenes via C═C Bond Cleavage or Oxygenation and Azidation of Open-Chain Alkenes.

J Org Chem 2022 Aug 2. Epub 2022 Aug 2.

School of Chemistry and Materials Science, Jiangsu Provincial Key Laboratory of Material Cycle Processes and Pollution Control, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Nanjing Normal University, Nanjing 210023, China.

An efficient strategy involving electrochemical C═C double-bond cleavage and functionalization of cyclic alkenes for the synthesis of ketonitriles is described. This transformation features environmentally friendly conditions and utilizes relatively safe TMSN as the nitrogenation reagent and molecular oxygen as the oxidant. For the open-chain alkenes, the reaction gave 1,2-difunctionalized products. A wide range of cyclic alkenes and open-chain alkenes were found to be compatible, providing the corresponding ketonitriles and α-azido aromatic ketones in moderate to good yields.
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http://dx.doi.org/10.1021/acs.joc.2c01293DOI Listing
August 2022

Low tidal volume ventilation alleviates ventilator-induced lung injury by regulating the NLRP3 inflammasome.

Exp Lung Res 2022 Aug 2:1-10. Epub 2022 Aug 2.

Department of Vascular and Thyroid Surgery, Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, People's Republic of China.

Purpose: Low tidal volume ventilation (LTVV) is a well-known ventilation mode which can improve ventilator-induced lung injury (VILI). However, the mechanism of LTVV ameliorating VILI has not yet been elucidated. In this study, we aimed to reveal LTVV protected against VILI by inhibiting the activation of the NLRP3 inflammasome in bronchoalveolar lavage fluid (BALF) from humans and lungs from mice.

Materials And Methods: Twenty-eight patients scheduled for video-assisted thoracoscopic esophagectomy were randomized to receive high-tidal-volume ventilation [ = 10 mL/kg without positive end-expiratory pressure (PEEP)] or LTVV ( = 5 mL/kg along with 5 cm of HO PEEP) during one-lung ventilation. BALF was collected before and at the end of surgery. Male C57BL/6 mice received high-tidal-volume ventilation, LTVV or MCC950 (an inhibitor of NLRP3). The activation of the formation of NLRP3 inflammasome in BALF from patients and in lungs from mice were analyzed.

Results: LTTV decreased the peak airway pressure (), plateau airway pressure () and driving pressure (Δ) during one-lung ventilation. Additionally, LTVV not only inhibited pulmonary infiltration and inflammation caused by mechanical ventilation, but also suppressed the NLRP3 inflammasome activation in BALF from humans. In mice, ventilator-induced inflammatory response and pulmonary edema were suppressed by LTVV with an efficacy comparable to that of MCC950 treatment. Furthermore, LTVV, similar to MCC950, clearly decreased ventilator-induced NLRP3 inflammasome activation.

Conclusion: Our study showed that LTVV played a protective role in ventilator-induced lung injury by suppressing the activation of the NLRP3 inflammasome.

Trial Registration: This study was registered in The Chinese Clinical Trial Registry, ChiCTR1900026190 on 25 September 2019.
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http://dx.doi.org/10.1080/01902148.2022.2104409DOI Listing
August 2022

The Influence of SiO + SiC + Al (HPO) Coating on Mechanical and Dielectric Properties for SiC/MWCNTS/AlPO Composites.

Materials (Basel) 2022 Jul 26;15(15). Epub 2022 Jul 26.

School of Materials Science and Engineering, Hunan University of Science and Technology, Xiangtan 411201, China.

SiC fiber-reinforcedAlPO matrix (SiC/MWCNTs/AlPO) composites were fabricated using a hot laminating process with multi-walled carbon nanotubes (MWCNTs) as the absorber. A coating prepared from SiO + SiC + Al (HPO) was applied to the surface of the SiC/MWCNTs/AlPO composites prior to an anti-oxidation test at 1273 K in air for 40 h. The anti-oxidation effect was verified by a three-point bending test, scanning electron microscopy, transmission electron microscopy, X-ray diffraction, and a dielectric property test. Anti-oxidation mechanism investigations revealed that the coating effectiveness could be attributed to three substances, i.e., SiO, SiPO and SiO +AlPO solid solution from the reactions of SiC + O→SiO + CO, SiO + PO→SiPO and SiO +AlPO→solid solution, respectively.
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http://dx.doi.org/10.3390/ma15155178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329869PMC
July 2022

Primary and Acquired Resistance against Immune Check Inhibitors in Non-Small Cell Lung Cancer.

Cancers (Basel) 2022 Jul 6;14(14). Epub 2022 Jul 6.

Department of Respiratory and Critical Care Medicine, Changhai Hospital, The First Affiliated Hospital of Naval Medical University (Second Military Medical University), Shanghai 200001, China.

Immune checkpoint inhibitors have emerged as the treatment landscape of advanced non-small cell lung cancer (NSCLC) in recent years. However, approximately 80% of NSCLC patients do not benefit from ICIs due to primary resistance (no initial response) or acquired resistance (tumor relapse after an initial response). In this review, we highlight the mechanisms of primary and secondary resistance. Furthermore, we provide a future direction of the potential predictive biomarkers and the tumor microenvironmental landscape and suggest treatment strategies to overcome these mechanisms.
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http://dx.doi.org/10.3390/cancers14143294DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316464PMC
July 2022

Characterization of Two Dehydrogenases from Involved in the Transformation of Patulin to Ascladiol.

Toxins (Basel) 2022 Jun 21;14(7). Epub 2022 Jun 21.

Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada.

Patulin is a mycotoxin that primarily contaminate apples and apple products. Whole cell or cell-free extracts of ATCC 621 were able to transform patulin to E-ascladiol. Proteins from cell-free extracts were separated by anion exchange chromatography and fractions with patulin transformation activity were subjected to peptide mass fingerprinting, enabling the identification of two NADPH dependent short chain dehydrogenases, GOX0525 and GOX1899, with the requisite activity. The genes encoding these enzymes were expressed in and purified. Kinetic parameters for patulin reduction, as well as pH profiles and thermostability were established to provide further insight on the potential application of these enzymes for patulin detoxification.
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http://dx.doi.org/10.3390/toxins14070423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323132PMC
June 2022

Direct Cyanation of Thiophenols or Thiols to Access Thiocyanates under Electrochemical Conditions.

J Org Chem 2022 Aug 22;87(15):10026-10033. Epub 2022 Jul 22.

School of Chemistry and Materials Science, Jiangsu Provincial Key Laboratory of Material Cycle Processes and Pollution Control, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Nanjing Normal University, Nanjing210023, China.

A novel electrochemical cross-coupling method for the synthesis of thiocyanates the direct cyanation of readily available thiophenols or thiols with trimethylsilyl cyanide (TMSCN) was developed. This approach was also suitable for selenols. External oxidant-free, transition-metal-free and mild operating conditions were the main advantages of this protocol. A series of thiocyanates and selenocyanates could be obtained in moderate to high yields.
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http://dx.doi.org/10.1021/acs.joc.2c00995DOI Listing
August 2022

Genomic Analysis of Mycobacterium abscessus Complex Isolates from Patients with Pulmonary Infection in China.

Microbiol Spectr 2022 Jul 12:e0011822. Epub 2022 Jul 12.

Department of Laboratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

Members of the Mycobacterium abscessus complex (MABC) are multidrug-resistant nontuberculous mycobacteria and increasingly cause opportunistic pulmonary infections. However, the genetic typing of MABC isolates remains largely unclear in China. Genomic analyses were conducted for 69 MABC clinical isolates obtained from patients with lower respiratory tract infections in Shanghai Pulmonary Hospital between 2014 and 2016. The draft genomes of the 69 clinical strains were assembled, with a total length of 4.5 to 5.6 Mb, a percent GC content (GC%) ranging from 63.9 to 68.1%, and 4,492 to 5,404 genes per genome. Susceptibility test shows that most isolates are resistant to many antimicrobials, including clarithromycin, but susceptible to tigecycline. Analyses revealed the presence of genes conferring resistance to antibiotics, including macrolides, aminoglycosides, rifampicin, and tetracyclines. Furthermore, 80 to 114 virulence genes were identified per genome, including those related to the invasion of macrophages, iron incorporation, and avoidance of immune clearance. Mobile genetic elements, including insertion sequences, transposons, and genomic islands, were discovered in the genomes. Phylogenetic analyses of all MABC isolates with another 41 complete MABC genomes identified three clades; 46 isolates were clustered in clade I, corresponding to M. abscessus subsp. , and 25 strains belonged to existing clonal complexes. Overall, this is the first comparative genomic analysis of MABC clinical isolates in China. These results show significant intraspecies variations in genetic determinants encoding antimicrobial resistance, virulence, and mobile elements and controversial subspecies classification using current marker gene combinations. This information will be useful in understanding the evolution, antimicrobial resistance, and pathogenesis of MABC strains and facilitating future vaccine development and drug design. Over the past decade, infections by Mycobacterium abscessus complex (MABC) isolates have been increasingly reported worldwide. MABC strains often show a high incidence in cystic fibrosis (CF) patients, whereas in Asia, these strains are frequently recovered from non-CF patients with significant genomic diversity. The present work involves analyses of the antimicrobial resistance, virulence, and phylogeny of 69 selected MABC isolates from non-CF pulmonary patients in Shanghai Pulmonary Hospital by whole-genome sequencing; it represents the first comprehensive investigation of MABC strains in China at the genomic level. These findings highlight the diversity of this group of nontuberculous mycobacteria and provide a mechanistic understanding of evolution and pathogenesis, which is valuable for the development of novel and effective antimicrobial therapies for deadly MABC infections in China.
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http://dx.doi.org/10.1128/spectrum.00118-22DOI Listing
July 2022

Emerging therapeutic potential of adeno-associated virus-mediated gene therapy in liver fibrosis.

Mol Ther Methods Clin Dev 2022 Sep 22;26:191-206. Epub 2022 Jun 22.

Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, Anhui Institute of Innovative Drugs, School of Pharmacy, Anhui Medical University, 81 Mei Shan Road, Hefei, Anhui Province 230032, China.

Liver fibrosis is a wound-healing response that results from various chronic damages. If the causes of damage are not removed or effective treatments are not given in a timely manner, it will progress to cirrhosis, even liver cancer. Currently, there are no specific medical therapies for liver fibrosis. Adeno-associated virus (AAV)-mediated gene therapy, one of the frontiers of modern medicine, has gained more attention in many fields due to its high safety profile, low immunogenicity, long-term efficacy in mediating gene expression, and increasingly known tropism. Notably, increasing evidence suggests a promising therapeutic potential for AAV-mediated gene therapy in different liver fibrosis models, which helps to correct abnormally changed target genes in the process of fibrosis and improve liver fibrosis at the molecular level. Moreover, the addition of cell-specific promoters to the genome of recombinant AAV helps to limit gene expression in specific cells, thereby producing better therapeutic efficacy in liver fibrosis. However, animal models are considered to be powerless predictive of tissue tropism, immunogenicity, and genotoxic risks in humans. Thus, AAV-mediated gene therapy will face many challenges. This review systemically summarizes the recent advances of AAV-mediated gene therapy in liver fibrosis, especially focusing on cellular and molecular mechanisms of transferred genes, and presents prospective challenges.
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http://dx.doi.org/10.1016/j.omtm.2022.06.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271983PMC
September 2022

Multiscale unsupervised domain adaptation for automatic pancreas segmentation in CT volumes using adversarial learning.

Med Phys 2022 Jul 14. Epub 2022 Jul 14.

Engineering Research Center of EMR and Intelligent Expert System, Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, China.

Purpose: Computer-aided automatic pancreas segmentation is essential for early diagnosis and treatment of pancreatic diseases. However, the annotation of pancreas images requires professional doctors and considerable expenditure. Due to imaging differences among various institution population, scanning devices, imaging protocols, and so on, significant degradation in the performance of model inference results is prone to occur when models trained with domain-specific (usually institution-specific) datasets are directly applied to new (other centers/institutions) domain data. In this paper, we propose a novel unsupervised domain adaptation method based on adversarial learning to address pancreas segmentation challenges with the lack of annotations and domain shift interference.

Methods: A 3D semantic segmentation model with attention module and residual module is designed as the backbone pancreas segmentation model. In both segmentation model and domain adaptation discriminator network, a multiscale progressively weighted structure is introduced to acquire different field of views. Features of labeled data and unlabeled data are fed in pairs into the proposed multiscale discriminator to learn domain-specific characteristics. Then the unlabeled data features with pseudodomain label are fed to the discriminator to acquire domain-ambiguous information. With this adversarial learning strategy, the performance of the segmentation network is enhanced to segment unseen unlabeled data.

Results: Experiments were conducted on two public annotated datasets as source datasets, respectively, and one private dataset as target dataset, where annotations were not used for the training process but only for evaluation. The 3D segmentation model achieves comparative performance with state-of-the-art pancreas segmentation methods on source domain. After implementing our domain adaptation architecture, the average dice similarity coefficient (DSC) of the segmentation model trained on the NIH-TCIA source dataset increases from 58.79% to 72.73% on the local hospital dataset, while the performance of the target domain segmentation model transferred from the medical segmentation decathlon (MSD) source dataset rises from 62.34% to 71.17%.

Conclusions: Correlations of features across data domains are utilized to train the pancreas segmentation model on unlabeled data domain, improving the generalization of the model. Our results demonstrate that the proposed method enables the segmentation model to make meaningful segmentation for unseen data of the training set. In the future, the proposed method has the potential to apply segmentation model trained on public dataset to clinical unannotated CT images from local hospital, effectively assisting radiologists in clinical practice.
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http://dx.doi.org/10.1002/mp.15827DOI Listing
July 2022

Effects of vacancy defects on the magnetic properties of vanadium diselenide monolayers: a first principle investigation.

Phys Chem Chem Phys 2022 Jul 27;24(29):17615-17622. Epub 2022 Jul 27.

Key Laboratory of Material Modification by Laser, Ion and Electron Beams, Dalian University of Technology, Ministry of Education, Dalian 116024, China.

Long-range ferromagnetic (FM) order in vanadium diselenide (VSe) monolayers (MLs) remains a controversial subject. In this theoretical study, we examined the effect of vacancy defects on the magnetic properties of octahedrally coordinated 1T-VSe MLs using spin-polarized density functional theory (DFT). In total, 45 different kinds of defects with various concentrations were introduced, including two single vacancies (S), 27 double vacancies (D), nine triple vacancies (T), and seven quadruple vacancies (Q). To understand the magnetic properties, S, D-(10), T-(10)(10), and Q-(21)(10)(10) were selected to be analyzed because they had low formation energies and large variations in magnetic moments (). Compared with the perfect VSe ML, the values of the of vanadium (V) decreased from 0.675 to 0.466, 0.183, 0.213, and 0.208 with increasing vacancy concentration. The same trend was also found for the energy differences between FM and antiferromagnetic (AFM) ordering. These results generally indicated weaker FM coupling and a lower Curie temperature in defective VSe. Vacancy-induced lattice distortion, d orbital shifting, electronic occupation, and spin density redistribution were discussed in order to explain the above observations. Our investigation demonstrated a strong dependence of and the magnetic interaction of VSe on the concentration and types of Se vacancies, which would explain the uncertainties encountered in magnetic experiments.
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http://dx.doi.org/10.1039/d2cp01537dDOI Listing
July 2022

The Practice and Application of AR Games to Assist Children's English Pronunciation Teaching.

Occup Ther Int 2022 30;2022:3966740. Epub 2022 Jun 30.

School of Foreign Languages, Hunan University of Technology and Business, Changsha, 410017 Hunan Province, China.

This paper conducts an in-depth study and analysis of the practice and application of teaching English pronunciation to children using AR (augmented reality) game assistance. The article analyzes the design of AR educational games based on the concept of learning behavior input, clarifies and determines the factors influencing students' learning behavior input in AR gamified teaching environment, and proposes the design principles of AR educational games based on learning behavior input. By analyzing the characteristics of experiential learning and AR, the model of AR-based English experiential learning activities for elementary school is established. Based on certain demand analysis, the AR software that can be used for teaching pronunciation in elementary school English classrooms was designed and developed using Unity3D and 3ds Max software. The experimental method was used to compare and apply the software in the target school, and the students of elementary school X were selected as the experimental subjects. The data were analyzed with the help of SPSS and NVivo software, and the questionnaire was used to measure the "ease of use," "usefulness," and "satisfaction with the software." The questionnaire was used to measure students' acceptance of AR software in three dimensions: "ease of use," "usefulness," and "satisfaction with the software."
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http://dx.doi.org/10.1155/2022/3966740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262547PMC
July 2022

LncRNA-Mediated Adipogenesis in Different Adipocytes.

Int J Mol Sci 2022 Jul 5;23(13). Epub 2022 Jul 5.

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

Long-chain noncoding RNAs (lncRNAs) are RNAs that do not code for proteins, widely present in eukaryotes. They regulate gene expression at multiple levels through different mechanisms at epigenetic, transcription, translation, and the maturation of mRNA transcripts or regulation of the chromatin structure, and compete with microRNAs for binding to endogenous RNA. Adipose tissue is a large and endocrine-rich functional tissue in mammals. Excessive accumulation of white adipose tissue in mammals can cause metabolic diseases. However, unlike white fat, brown and beige fats release energy as heat. In recent years, many lncRNAs associated with adipogenesis have been reported. The molecular mechanisms of how lncRNAs regulate adipogenesis are continually investigated. In this review, we discuss the classification of lncRNAs according to their transcriptional location. lncRNAs that participate in the adipogenesis of white or brown fats are also discussed. The function of lncRNAs as decoy molecules and RNA double-stranded complexes, among other functions, is also discussed.
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http://dx.doi.org/10.3390/ijms23137488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267348PMC
July 2022

3-keto-DON, but Not 3--DON, Retains the Toxicological Potential after the Enzymatic Biotransformation of Deoxynivalenol.

Int J Mol Sci 2022 Jun 29;23(13). Epub 2022 Jun 29.

Guelph Research and Development Centre, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, ON N1G 5C9, Canada.

Deoxynivalenol (DON) is a secondary fungal metabolite that is associated with many adverse toxicological effects in agriculture as well as human/animal nutrition. Bioremediation efforts in recent years have led to the discovery of numerous bacterial isolates that can transform DON to less toxic derivatives. Both 3-keto-DON and 3--DON were recently shown to exhibit reduced toxicity, compared to DON, when tested using different cell lines and mammalian models. In the current study, the toxicological assessment of 3-keto-DON and 3--DON using models surprisingly revealed that 3-keto-DON, but not 3--DON, retained its toxicity to a large extent in both duckweeds ( L.) and common wheat ( L.) model systems. RNA-Seq analysis revealed that the exposure of to 3-keto-DON and DON resulted in substantial transcriptomic changes and similar gene expression profiles, whereas 3--DON did not. These novel findings are pivotal for understanding the environmental burden of the above metabolites as well as informing the development of future transgenic plant applications. Collectively, they emphasize the fundamental need to assess both plant and animal models when evaluating metabolites/host interactions.
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http://dx.doi.org/10.3390/ijms23137230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266554PMC
June 2022

Gut Microbiota Composition and Diversity in Different Commercial Swine Breeds in Early and Finishing Growth Stages.

Animals (Basel) 2022 Jun 22;12(13). Epub 2022 Jun 22.

Department of Animal Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

The gut microbiota affects the metabolism, health and growth rate of pigs. Understanding the characteristics of gut microbiota of different pig breeds at each growth stage will enable the design of individualized feeding strategies. The present study aimed to compare the growth curves and development patterns of pigs of three different breeds (Duroc, Landrace and Yorkshire) using the mathematical models Gompertz, Logistic, Von Bertalanffy and Richards. For Duroc pigs, the Gompertz model showed the highest prediction accuracy (R = 0.9974). In contrast, the best models for Landrace and Yorkshire pigs were Richards (R = 0.9986) and Von Bertalanffy (R = 0.9977), respectively. Path analysis showed that body length (path coefficient  =  0.507) and chest circumference (path coefficient  =  0.532) contributed more significantly to the body weight of pigs at the early growth stage, while hip circumference (path coefficient  =  0.312) had a greater influence on pig body weight in the late growth stage. Moreover, the composition of the gut microbiota of pigs at two growth stages (60 kg of body weight in the early growth stage and 120 kg in the finishing stage) was studied using 16S rRNA sequencing technology. Variations in gut microbiota composition of pigs at different growth stages were observed. KEGG pathway enrichment analysis of annotated metagenomes revealed that protein synthesis and amino acid metabolism pathways were significantly enriched in pigs at the early growth stage, which may be related to nutritional requirements of pigs during this stage. This study confirmed longitudinal variation in the gut microbiota of pigs pertaining to age as well as lateral variation related to pig breed. The present findings expand the current understanding of the variations in swine gut microbiota during production stages.
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http://dx.doi.org/10.3390/ani12131607DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264831PMC
June 2022

Expression and role of ABIN1 in sepsis: and studies.

Open Med (Wars) 2022 11;17(1):1064. Epub 2022 Jun 11.

Department of ICU, Zaozhuang Municipal Hospital, Zaozhuang 277102, China.

[This retracts the article DOI: 10.1515/med-2021-0008.].
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http://dx.doi.org/10.1515/med-2022-0503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9191561PMC
June 2022

FDG PET/CT in a Case With Primary Poorly Differentiated Urothelial-Type Adenocarcinoma of the Prostate.

Clin Nucl Med 2022 Aug 4;47(8):736-738. Epub 2022 Apr 4.

From the Departments of Nuclear Medicine.

Abstract: Primary urothelial-type adenocarcinoma of the prostate is extremely rare. We describe FDG PET/CT findings in a case with primary poorly differentiated urothelial-type adenocarcinoma of the prostate with pelvic lymph node metastases. Both the prostate tumor and its metastatic lesions showed intense FDG uptake. The patient had normal serum prostate-specific antigen and elevated carcinoembryonic antigen levels, which may be helpful for the differential diagnosis.
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http://dx.doi.org/10.1097/RLU.0000000000004150DOI Listing
August 2022

[Clinicopathological Characteristics of Lymphadenitis Caused by Diagnosed by Core Needle Biopsy].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2022 Jun;44(3):440-445

Department of Pathology,,Quanzhou First Hospital Affiliated to Fujian Medical University,Quanzhou,Fujian 362000,China.

Objective To analyze the clinicopathological characteristics of lymphadenitis caused by (TM).Method s The clinical data,pathological features,pathogen examination,and treatment of 15 cases of TM-caused lymphadenitis were analyzed retrospectively.Results The 15 cases included 14 males and 1 females,who were aged 26-67 years,with an average age of (49.1±11.87) years.The 15 cases,including 13 cases of acquired immunodeficiency syndrome and 2 cases of diabetes mellitus,were accompanied by superficial lymph node enlargement in the neck and supraclavicular,axillary,and inguinal regions.The structure of cord-like lymph node tissue punctured by thick needle was completely or partially replaced by inflammatory lesions. Under microscope,8 cases showed mainly diffuse infiltration of phagocytes with pathogens;5 cases presented mainly extensive coagulation necrosis with a small amount of pathogens and nuclear debris;2 cases were characterized by small nodular hyperplasia of fibroblasts,formation of granulomatous structure,and scattered distribution of a few multinucleated giant cells.The pathogens were relatively consistent in size and shape,which were round,oval or sausage-shaped and clustered like mulberry.Diastase periodic acid-Schiff staining and hexamine silver staining highlighted the bacterial structure with transverse septum.TM growth was detected in the blood,alveolar lavage fluid,sputum or lymph node extract fungal culture of the 15 patients.Owing to the adequate antifungal treatment in time,these 15 patients were discharged after their conditions were improved.Conclusion Lymphadenitis is one of the major manifestations of the systemic invasion of TM at the late stage,which is tended to be misdiagnosed.Through core needle biopsy of lymph node,it can be diagnosed as soon as possible to avoid delayed treatment and improve the cure rate.
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http://dx.doi.org/10.3881/j.issn.1000-503X.14639DOI Listing
June 2022

LncRNA NDRG1 aggravates osteosarcoma progression and regulates the PI3K/AKT pathway by sponging miR-96-5p.

BMC Cancer 2022 Jul 4;22(1):728. Epub 2022 Jul 4.

Department of Orthopaedics, Jinling Hospital, Nanjing Medical University, Zhongshan Road 305, Nanjing, 210002, China.

Background: Osteosarcoma (OS) is the most common primary malignant bone tumors in children and adolescents. Large numbers of studies have focused on the long non-coding RNA (lncRNA) that plays essential roles in the progression of osteosarcoma. Nevertheless, the functions and underlying mechanisms of LncRNA NDRG1 in osteosarcoma remain unknown.

Methods: Differentially expressed lncRNAs between osteosarcoma and adjacent normal tissues were identified through RNA sequencing. The role of LncRNA NDRG1 in osteosarcoma proliferation and metastasis were investigated through in vitro and in vivo functional experiments. The interaction between LncRNA NDRG1 and miR-96-5p was verified through bioinformatic analysis and luciferase reporter assay. Regulation relationship between LncRNA NDRG1 and miR-96-5p was further evaluated by the rescue experiments. Additionally, the changes in the expression of epithelial-mesenchymal transition (EMT) and the PI3K/AKT pathway were verified by Western blot.

Results: LncRNA NDRG1 was up-regulated in osteosarcoma cell lines and tissues and the expression of LncRNA NDRG1 was correlated with the overall survival of osteosarcoma patients. Functional experiments exhibited that LncRNA NDRG1 aggravated osteosarcoma proliferation and migration in vitro; meanwhile, animals experiments showed that LncRNA NDRG1 promoted osteosarcoma growth and metastasis in vivo. Mechanistically, LncRNA NDRG1 was found to aggravate osteosarcoma progression and regulate the PI3K/AKT pathway by sponging miR-96-5p.

Conclusions: LncRNA NDRG1 aggravates osteosarcoma progression and regulates the PI3K/AKT pathway by sponging miR-96-5p. Therefore, LncRNA NDRG1 could act as a prognostic marker and a therapeutic target for osteosarcoma in the future.
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http://dx.doi.org/10.1186/s12885-022-09833-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252029PMC
July 2022

Exogenous metabolite feeding on altering antibiotic susceptibility in Gram-negative bacteria through metabolic modulation: a review.

Metabolomics 2022 07 4;18(7):47. Epub 2022 Jul 4.

Faculty of Pharmacy, University of Malaya, 50603, Kuala Lumpur, Malaysia.

Background: The rise of antimicrobial resistance at an alarming rate is outpacing the development of new antibiotics. The worrisome trends of multidrug-resistant Gram-negative bacteria have enormously diminished existing antibiotic activity. Antibiotic treatments may inhibit bacterial growth or lead to induce bacterial cell death through disruption of bacterial metabolism directly or indirectly. In light of this, it is imperative to have a thorough understanding of the relationship of bacterial metabolism with antimicrobial activity and leverage the underlying principle towards development of novel and effective antimicrobial therapies.

Objective: Herein, we explore studies on metabolic analyses of Gram-negative pathogens upon antibiotic treatment. Metabolomic studies revealed that antibiotic therapy caused changes of metabolites abundance and perturbed the bacterial metabolism. Following this line of thought, addition of exogenous metabolite has been employed in in vitro, in vivo and in silico studies to activate the bacterial metabolism and thus potentiate the antibiotic activity.

Key Scientific Concepts Of Review: Exogenous metabolites were discovered to cause metabolic modulation through activation of central carbon metabolism and cellular respiration, stimulation of proton motive force, increase of membrane potential, improvement of host immune protection, alteration of gut microbiome, and eventually facilitating antibiotic killing. The use of metabolites as antimicrobial adjuvants may be a promising approach in the fight against multidrug-resistant pathogens.
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http://dx.doi.org/10.1007/s11306-022-01903-wDOI Listing
July 2022

Qishen Yiqi Dropping Pill facilitates post-stroke recovery of motion and memory loss by modulating ICAM-1-mediated neuroinflammation.

Biomed Pharmacother 2022 Jun 27;153:113325. Epub 2022 Jun 27.

State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Beihua South Road, JingHai District, Tianjin 301617, China; Research and Development Center of TCM, Tianjin International Joint Academy of Biomedicine, 220 Dongting Road, TEDA, Tianjin 300457, China. Electronic address:

Background: Promotion of functional recovery in patients is the primary goal of stroke management. However, its achievement is low due to the lack of full understanding of the complex pathological process of stroke and therefore limited therapeutic strategies. Qishen Yiqi Dropping Pill (QSYQ) is a component-based Chinese medicine that has been widely used in clinical treatment of ischemic cardiovascular diseases. Our previous studies indicated that QSYQ were protective for acute ischemic stroke in animal models and this study aimed to investigate the effect of QSYQ on brain function during stroke recovery.

Methods: The therapeutic effects of QSYQ were evaluated by neurological deficit score, dark avoidance test, gait analysis, Morris water maze and brain tissue atrophy volume in a rat model of middle cerebral artery occlusion (MCAO) with ischemia for 60 min. The underlying mechanisms of QSYQ accelerating the functional recovery of MCAO rats was then revealed using proteomic sequencing and validated by immunohistochemistry, qRT-PCR and ELISA assays. The active components in QSYQ were elucidated by molecular docking and verified biochemically in vitro.

Results: QSYQ treatment for 28 days significantly improved the neurological function, gait, spontaneous activity, spatial memory, and reduced brain atrophy in MCAO rats. Proteomic analysis of ischemic brain region and the following bioinformatics studies showed that QSYQ intervention markedly modulated neuroinflammatory responses post stroke, in which ICAM-1 played a dominant role. In particular, QSYQ reversed high cerebral expression of ICAM-1 in MCAO rats and decreased the content of TNF-α, IL-6 and IL1β in brain tissue and serum. In vitro, it was found that the active component rosmarinic acid in QSYQ evidently inhibit the expression of ICAM-1 caused by oxygen glucose deprivation/reoxygenation injury via using immunofluorescence staining.

Conclusion: QSYQ effectively accelerates the recovery of motor impairment and memory loss in rats after stroke via downregulation of ICAM-1-mediated neuroinflammation, and rosmarinic acid is one of its main active components.
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http://dx.doi.org/10.1016/j.biopha.2022.113325DOI Listing
June 2022

A brief review of protein-ligand interaction prediction.

Comput Struct Biotechnol J 2022 3;20:2831-2838. Epub 2022 Jun 3.

College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, China.

The task of identifying protein-ligand interactions (PLIs) plays a prominent role in the field of drug discovery. However, it is infeasible to identify potential PLIs via costly and laborious experiments. There is a need to develop PLI computational prediction approaches to speed up the drug discovery process. In this review, we summarize a brief introduction to various computation-based PLIs. We discuss these approaches, in particular, machine learning-based methods, with illustrations of different emphases based on mainstream trends. Moreover, we analyzed three research dynamics that can be further explored in future studies.
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http://dx.doi.org/10.1016/j.csbj.2022.06.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189993PMC
June 2022

Improving Estimation of Winter Wheat Nitrogen Status Using Random Forest by Integrating Multi-Source Data Across Different Agro-Ecological Zones.

Front Plant Sci 2022 10;13:890892. Epub 2022 Jun 10.

MARA Key Laboratory for Crop System Analysis and Decision Making, Jiangsu Key Laboratory for Information Agriculture, National Engineering and Technology Center for Information Agriculture, MOE Engineering and Research Center for Smart Agriculture, Collaborative Innovation Center for Modern Crop Production Co-sponsored by Province and Ministry, Nanjing Agricultural University, Nanjing, China.

Timely and accurate estimation of plant nitrogen (N) status is crucial to the successful implementation of precision N management. It has been a great challenge to non-destructively estimate plant N status across different agro-ecological zones (AZs). The objective of this study was to use random forest regression (RFR) models together with multi-source data to improve the estimation of winter wheat ( L.) N status across two AZs. Fifteen site-year plot and farmers' field experiments involving different N rates and 19 cultivars were conducted in two AZs from 2015 to 2020. The results indicated that RFR models integrating climatic and management factors with vegetation index (R = 0.72-0.86) outperformed the models by only using the vegetation index (R = 0.36-0.68) and performed well across AZs. The Pearson correlation coefficient-based variables selection strategy worked well to select 6-7 key variables for developing RFR models that could achieve similar performance as models using full variables. The contributions of climatic and management factors to N status estimation varied with AZs and N status indicators. In higher-latitude areas, climatic factors were more important to N status estimation, especially water-related factors. The addition of climatic factors significantly improved the performance of the RFR models for N nutrition index estimation. Climatic factors were important for the estimation of the aboveground biomass, while management variables were more important to N status estimation in lower-latitude areas. It is concluded that integrating multi-source data using RFR models can significantly improve the estimation of winter wheat N status indicators across AZs compared to models only using one vegetation index. However, more studies are needed to develop unmanned aerial vehicles and satellite remote sensing-based machine learning models incorporating multi-source data for more efficient monitoring of crop N status under more diverse soil, climatic, and management conditions across large regions.
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http://dx.doi.org/10.3389/fpls.2022.890892DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226625PMC
June 2022
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